BLASTX nr result
ID: Astragalus24_contig00008539
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Astragalus24_contig00008539
(405 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
149,584,005 sequences; 54,822,741,787 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AHG97656.1| isoflavone-2'-hydroxylase [Astragalus mongholicus] 146 6e-39
gb|KHN28917.1| Isoflavone 2'-hydroxylase, partial [Glycine soja] 139 2e-37
gb|PNX57318.1| isoflavone 2'-hydroxylase-like protein, partial [... 130 4e-37
ref|XP_014506446.2| isoflavone 2'-hydroxylase [Vigna radiata var... 140 1e-36
ref|XP_017405614.1| PREDICTED: isoflavone 2'-hydroxylase-like [V... 139 3e-36
gb|KOM25514.1| hypothetical protein LR48_Vigan107s003300 [Vigna ... 139 3e-36
ref|XP_003532672.1| PREDICTED: isoflavone 2'-hydroxylase-like [G... 139 4e-36
dbj|GAU13746.1| hypothetical protein TSUD_82550 [Trifolium subte... 135 1e-35
gb|AFK44067.1| unknown [Medicago truncatula] 128 2e-34
ref|XP_007158857.1| hypothetical protein PHAVU_002G187700g [Phas... 134 3e-34
dbj|GAU13742.1| hypothetical protein TSUD_82510 [Trifolium subte... 130 1e-33
gb|PNX82814.1| isoflavone 2'-hydroxylase-like protein, partial [... 125 1e-33
dbj|BAT21113.1| cytochrome P450 [synthetic construct] 130 3e-33
sp|P93147.2|C81E1_GLYEC RecName: Full=Isoflavone 2'-hydroxylase;... 130 4e-33
dbj|BAA74465.1| cytochrome P450 [Glycyrrhiza echinata] 130 4e-33
ref|XP_013457402.1| cytochrome P450 family 81 protein [Medicago ... 128 3e-32
dbj|BAT21114.1| cytochrome P450 [synthetic construct] 125 2e-31
dbj|BAA93634.1| cytochrome P450 [Lotus japonicus] 125 3e-31
ref|NP_001296581.1| isoflavone 2'-hydroxylase [Cicer arietinum] ... 124 7e-31
ref|XP_016191270.1| isoflavone 2'-hydroxylase [Arachis ipaensis] 124 7e-31
>gb|AHG97656.1| isoflavone-2'-hydroxylase [Astragalus mongholicus]
Length = 498
Score = 146 bits (368), Expect = 6e-39
Identities = 71/83 (85%), Positives = 75/83 (90%)
Frame = -3
Query: 403 RFEKEGEIDKLIAFGLGRRACPGGALAMRAISLTLGLLVQCFDWKLVDDKEIDMREESGF 224
RF+KEGE++KLIAFGLGRRACPGGALAMRAISLTLGLLVQCFDWKLVDDKEIDMREESGF
Sbjct: 416 RFDKEGELEKLIAFGLGRRACPGGALAMRAISLTLGLLVQCFDWKLVDDKEIDMREESGF 475
Query: 223 TLTMLVPLKAMCKSRPDINNLFK 155
TL+ L PL AMCK RP I L K
Sbjct: 476 TLSKLTPLTAMCKLRPAIKKLVK 498
>gb|KHN28917.1| Isoflavone 2'-hydroxylase, partial [Glycine soja]
Length = 329
Score = 139 bits (349), Expect = 2e-37
Identities = 66/83 (79%), Positives = 74/83 (89%)
Frame = -3
Query: 403 RFEKEGEIDKLIAFGLGRRACPGGALAMRAISLTLGLLVQCFDWKLVDDKEIDMREESGF 224
RFEKEGE+DKLIAFGLGRRACPG LAMRA+ L+LGLL+QCF+WK V DKEIDMREESGF
Sbjct: 247 RFEKEGELDKLIAFGLGRRACPGEGLAMRALCLSLGLLIQCFEWKRVGDKEIDMREESGF 306
Query: 223 TLTMLVPLKAMCKSRPDINNLFK 155
TL+ L+PLKAMCK+RP IN L K
Sbjct: 307 TLSRLIPLKAMCKARPVINRLGK 329
>gb|PNX57318.1| isoflavone 2'-hydroxylase-like protein, partial [Trifolium
pratense]
Length = 90
Score = 130 bits (328), Expect = 4e-37
Identities = 63/83 (75%), Positives = 70/83 (84%)
Frame = -3
Query: 403 RFEKEGEIDKLIAFGLGRRACPGGALAMRAISLTLGLLVQCFDWKLVDDKEIDMREESGF 224
RF+K+GE +KLIAFG+GRRACPG ALAMRAIS+TL LLVQCFDWKL D +IDM E GF
Sbjct: 7 RFDKKGESEKLIAFGMGRRACPGEALAMRAISMTLALLVQCFDWKLTGDGKIDMSERDGF 66
Query: 223 TLTMLVPLKAMCKSRPDINNLFK 155
TLT LVPLKAMCK+RP IN L K
Sbjct: 67 TLTKLVPLKAMCKTRPVINKLLK 89
>ref|XP_014506446.2| isoflavone 2'-hydroxylase [Vigna radiata var. radiata]
Length = 504
Score = 140 bits (352), Expect = 1e-36
Identities = 65/81 (80%), Positives = 73/81 (90%)
Frame = -3
Query: 403 RFEKEGEIDKLIAFGLGRRACPGGALAMRAISLTLGLLVQCFDWKLVDDKEIDMREESGF 224
R+E EGE++KLIAFGLGRRACPGGALAMRA+ LTLGLL+QCFDWKLV DKE+DMREE GF
Sbjct: 422 RYESEGELEKLIAFGLGRRACPGGALAMRALCLTLGLLIQCFDWKLVGDKELDMREEDGF 481
Query: 223 TLTMLVPLKAMCKSRPDINNL 161
TL+ L+PLKAMCK RP IN L
Sbjct: 482 TLSRLIPLKAMCKPRPLINKL 502
>ref|XP_017405614.1| PREDICTED: isoflavone 2'-hydroxylase-like [Vigna angularis]
Length = 500
Score = 139 bits (350), Expect = 3e-36
Identities = 64/81 (79%), Positives = 74/81 (91%)
Frame = -3
Query: 403 RFEKEGEIDKLIAFGLGRRACPGGALAMRAISLTLGLLVQCFDWKLVDDKEIDMREESGF 224
RFE EGE++KLIAFG+GRRACPGGALAMRA+ LTLGLL+QCFDWKL DKE+DMREE+GF
Sbjct: 418 RFESEGELEKLIAFGMGRRACPGGALAMRALCLTLGLLIQCFDWKLFGDKELDMREENGF 477
Query: 223 TLTMLVPLKAMCKSRPDINNL 161
TL+ L+PLKAMCK+RP IN L
Sbjct: 478 TLSRLIPLKAMCKARPVINML 498
>gb|KOM25514.1| hypothetical protein LR48_Vigan107s003300 [Vigna angularis]
dbj|BAT74198.1| hypothetical protein VIGAN_01181700 [Vigna angularis var.
angularis]
Length = 504
Score = 139 bits (350), Expect = 3e-36
Identities = 64/81 (79%), Positives = 74/81 (91%)
Frame = -3
Query: 403 RFEKEGEIDKLIAFGLGRRACPGGALAMRAISLTLGLLVQCFDWKLVDDKEIDMREESGF 224
RFE EGE++KLIAFG+GRRACPGGALAMRA+ LTLGLL+QCFDWKL DKE+DMREE+GF
Sbjct: 422 RFESEGELEKLIAFGMGRRACPGGALAMRALCLTLGLLIQCFDWKLFGDKELDMREENGF 481
Query: 223 TLTMLVPLKAMCKSRPDINNL 161
TL+ L+PLKAMCK+RP IN L
Sbjct: 482 TLSRLIPLKAMCKARPVINML 502
>ref|XP_003532672.1| PREDICTED: isoflavone 2'-hydroxylase-like [Glycine max]
gb|KRH42431.1| hypothetical protein GLYMA_08G089500 [Glycine max]
Length = 502
Score = 139 bits (349), Expect = 4e-36
Identities = 66/83 (79%), Positives = 74/83 (89%)
Frame = -3
Query: 403 RFEKEGEIDKLIAFGLGRRACPGGALAMRAISLTLGLLVQCFDWKLVDDKEIDMREESGF 224
RFEKEGE+DKLIAFGLGRRACPG LAMRA+ L+LGLL+QCF+WK V DKEIDMREESGF
Sbjct: 420 RFEKEGELDKLIAFGLGRRACPGEGLAMRALCLSLGLLIQCFEWKRVGDKEIDMREESGF 479
Query: 223 TLTMLVPLKAMCKSRPDINNLFK 155
TL+ L+PLKAMCK+RP IN L K
Sbjct: 480 TLSRLIPLKAMCKARPVINRLGK 502
>dbj|GAU13746.1| hypothetical protein TSUD_82550 [Trifolium subterraneum]
Length = 393
Score = 135 bits (340), Expect = 1e-35
Identities = 66/81 (81%), Positives = 70/81 (86%)
Frame = -3
Query: 403 RFEKEGEIDKLIAFGLGRRACPGGALAMRAISLTLGLLVQCFDWKLVDDKEIDMREESGF 224
RFEKEGE+ KLIAFGLGRRACPGG LAMRAI LTLGLL+QCF+WK VDDKEIDM EESGF
Sbjct: 311 RFEKEGELAKLIAFGLGRRACPGGGLAMRAICLTLGLLIQCFEWKKVDDKEIDMSEESGF 370
Query: 223 TLTMLVPLKAMCKSRPDINNL 161
TL+ VPLKAMCK RP I L
Sbjct: 371 TLSRSVPLKAMCKVRPVIKKL 391
>gb|AFK44067.1| unknown [Medicago truncatula]
Length = 227
Score = 128 bits (322), Expect = 2e-34
Identities = 56/83 (67%), Positives = 73/83 (87%)
Frame = -3
Query: 403 RFEKEGEIDKLIAFGLGRRACPGGALAMRAISLTLGLLVQCFDWKLVDDKEIDMREESGF 224
RF+K+GE++K+IAFG+GRRACPG LA+RAIS+TL LLVQCFDWK ++D++IDM E GF
Sbjct: 145 RFDKKGELEKMIAFGMGRRACPGEGLALRAISMTLALLVQCFDWKRINDEKIDMSERDGF 204
Query: 223 TLTMLVPLKAMCKSRPDINNLFK 155
T+T L+PLKAMCK+RP +N +FK
Sbjct: 205 TMTKLLPLKAMCKTRPVVNKVFK 227
>ref|XP_007158857.1| hypothetical protein PHAVU_002G187700g [Phaseolus vulgaris]
gb|ESW30851.1| hypothetical protein PHAVU_002G187700g [Phaseolus vulgaris]
Length = 500
Score = 134 bits (336), Expect = 3e-34
Identities = 63/81 (77%), Positives = 72/81 (88%)
Frame = -3
Query: 403 RFEKEGEIDKLIAFGLGRRACPGGALAMRAISLTLGLLVQCFDWKLVDDKEIDMREESGF 224
RFE EGE++KLIAFGLGRRACPGGALAMRA+ LTLGLL+QCFDWK+V DKE+DMREE+GF
Sbjct: 418 RFENEGELEKLIAFGLGRRACPGGALAMRALCLTLGLLIQCFDWKVVGDKELDMREENGF 477
Query: 223 TLTMLVPLKAMCKSRPDINNL 161
TL+ L+PLK M K RP IN L
Sbjct: 478 TLSRLIPLKLMFKVRPLINKL 498
>dbj|GAU13742.1| hypothetical protein TSUD_82510 [Trifolium subterraneum]
Length = 389
Score = 130 bits (327), Expect = 1e-33
Identities = 61/81 (75%), Positives = 70/81 (86%)
Frame = -3
Query: 403 RFEKEGEIDKLIAFGLGRRACPGGALAMRAISLTLGLLVQCFDWKLVDDKEIDMREESGF 224
RFEK+GE++K+IAFG+GRRACPG ALAMRAIS+TL LLVQCFDWKL D +IDM E GF
Sbjct: 306 RFEKKGELEKMIAFGMGRRACPGEALAMRAISMTLALLVQCFDWKLTGDGKIDMTERDGF 365
Query: 223 TLTMLVPLKAMCKSRPDINNL 161
TLT LVPLKAMCK+RP +N L
Sbjct: 366 TLTKLVPLKAMCKTRPVVNKL 386
>gb|PNX82814.1| isoflavone 2'-hydroxylase-like protein, partial [Trifolium
pratense]
Length = 201
Score = 125 bits (314), Expect = 1e-33
Identities = 59/83 (71%), Positives = 69/83 (83%)
Frame = -3
Query: 403 RFEKEGEIDKLIAFGLGRRACPGGALAMRAISLTLGLLVQCFDWKLVDDKEIDMREESGF 224
RF+K+GE++KLIAFG+GRRACPG LAMRAIS+TL LLVQCFDW+L D +IDM E+ GF
Sbjct: 119 RFDKKGELEKLIAFGIGRRACPGETLAMRAISMTLALLVQCFDWELTSDGKIDMVEQDGF 178
Query: 223 TLTMLVPLKAMCKSRPDINNLFK 155
L VPLKAMCK+RP IN LFK
Sbjct: 179 ALKKSVPLKAMCKTRPVINKLFK 201
>dbj|BAT21113.1| cytochrome P450 [synthetic construct]
Length = 477
Score = 130 bits (328), Expect = 3e-33
Identities = 60/83 (72%), Positives = 72/83 (86%)
Frame = -3
Query: 403 RFEKEGEIDKLIAFGLGRRACPGGALAMRAISLTLGLLVQCFDWKLVDDKEIDMREESGF 224
RFEK+GE++KLIAFG+GRRACPG LA+RAIS+TL LL+QCFDWKL++ +ID+ E GF
Sbjct: 394 RFEKKGELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDGF 453
Query: 223 TLTMLVPLKAMCKSRPDINNLFK 155
TLT LVPLKAMCKSRP IN +FK
Sbjct: 454 TLTKLVPLKAMCKSRPVINKVFK 476
>sp|P93147.2|C81E1_GLYEC RecName: Full=Isoflavone 2'-hydroxylase; AltName: Full=CYP GE-3;
AltName: Full=Cytochrome P450 81E1; AltName:
Full=Cytochrome P450 91A4
dbj|BAA22422.1| cytochrome P450 [Glycyrrhiza echinata]
Length = 499
Score = 130 bits (328), Expect = 4e-33
Identities = 60/83 (72%), Positives = 72/83 (86%)
Frame = -3
Query: 403 RFEKEGEIDKLIAFGLGRRACPGGALAMRAISLTLGLLVQCFDWKLVDDKEIDMREESGF 224
RFEK+GE++KLIAFG+GRRACPG LA+RAIS+TL LL+QCFDWKL++ +ID+ E GF
Sbjct: 416 RFEKKGELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDGF 475
Query: 223 TLTMLVPLKAMCKSRPDINNLFK 155
TLT LVPLKAMCKSRP IN +FK
Sbjct: 476 TLTKLVPLKAMCKSRPVINKVFK 498
>dbj|BAA74465.1| cytochrome P450 [Glycyrrhiza echinata]
Length = 499
Score = 130 bits (328), Expect = 4e-33
Identities = 61/83 (73%), Positives = 71/83 (85%)
Frame = -3
Query: 403 RFEKEGEIDKLIAFGLGRRACPGGALAMRAISLTLGLLVQCFDWKLVDDKEIDMREESGF 224
RFEK+GE++KLIAFG+GRRACPG LA+RAIS+TL LL+QCFDWKL + +IDM E GF
Sbjct: 416 RFEKKGELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLTNGDKIDMAERDGF 475
Query: 223 TLTMLVPLKAMCKSRPDINNLFK 155
TLT LVPLKAMCKSRP IN +FK
Sbjct: 476 TLTKLVPLKAMCKSRPVINKVFK 498
>ref|XP_013457402.1| cytochrome P450 family 81 protein [Medicago truncatula]
sp|Q6WNR0.1|C81E7_MEDTR RecName: Full=Isoflavone 2'-hydroxylase; AltName:
Full=4'-methoxyisoflavone 2'-hydroxylase; AltName:
Full=Cytochrome P450 81E7
gb|AAQ20040.1| isoflavone 2'-hydroxylase [Medicago truncatula]
gb|KEH31433.1| cytochrome P450 family 81 protein [Medicago truncatula]
Length = 498
Score = 128 bits (322), Expect = 3e-32
Identities = 56/83 (67%), Positives = 73/83 (87%)
Frame = -3
Query: 403 RFEKEGEIDKLIAFGLGRRACPGGALAMRAISLTLGLLVQCFDWKLVDDKEIDMREESGF 224
RF+K+GE++K+IAFG+GRRACPG LA+RAIS+TL LLVQCFDWK ++D++IDM E GF
Sbjct: 416 RFDKKGELEKMIAFGMGRRACPGEGLALRAISMTLALLVQCFDWKRINDEKIDMSERDGF 475
Query: 223 TLTMLVPLKAMCKSRPDINNLFK 155
T+T L+PLKAMCK+RP +N +FK
Sbjct: 476 TMTKLLPLKAMCKTRPVVNKVFK 498
>dbj|BAT21114.1| cytochrome P450 [synthetic construct]
Length = 477
Score = 125 bits (315), Expect = 2e-31
Identities = 58/83 (69%), Positives = 69/83 (83%)
Frame = -3
Query: 403 RFEKEGEIDKLIAFGLGRRACPGGALAMRAISLTLGLLVQCFDWKLVDDKEIDMREESGF 224
RF+K+GE++KLI FGLGRRACPG LA+RAIS+TL LL+QCFDWK V D+EIDM E GF
Sbjct: 395 RFDKKGELEKLIPFGLGRRACPGELLAIRAISMTLALLIQCFDWKRVSDEEIDMGERDGF 454
Query: 223 TLTMLVPLKAMCKSRPDINNLFK 155
L +P+KAMCKSRP INN+FK
Sbjct: 455 VLMKSIPVKAMCKSRPVINNVFK 477
>dbj|BAA93634.1| cytochrome P450 [Lotus japonicus]
Length = 499
Score = 125 bits (315), Expect = 3e-31
Identities = 58/83 (69%), Positives = 69/83 (83%)
Frame = -3
Query: 403 RFEKEGEIDKLIAFGLGRRACPGGALAMRAISLTLGLLVQCFDWKLVDDKEIDMREESGF 224
RF+K+GE++KLI FGLGRRACPG LA+RAIS+TL LL+QCFDWK V D+EIDM E GF
Sbjct: 417 RFDKKGELEKLIPFGLGRRACPGELLAIRAISMTLALLIQCFDWKRVSDEEIDMGERDGF 476
Query: 223 TLTMLVPLKAMCKSRPDINNLFK 155
L +P+KAMCKSRP INN+FK
Sbjct: 477 VLMKSIPVKAMCKSRPVINNVFK 499
>ref|NP_001296581.1| isoflavone 2'-hydroxylase [Cicer arietinum]
emb|CAA10067.1| cytochrome P450 [Cicer arietinum]
Length = 498
Score = 124 bits (312), Expect = 7e-31
Identities = 56/83 (67%), Positives = 68/83 (81%)
Frame = -3
Query: 403 RFEKEGEIDKLIAFGLGRRACPGGALAMRAISLTLGLLVQCFDWKLVDDKEIDMREESGF 224
RF+K+GEI+K+IAFG+GRR CPG ALA+R IS+TL LLVQCFDWK D +IDM E GF
Sbjct: 416 RFDKKGEIEKVIAFGMGRRVCPGEALALRTISMTLALLVQCFDWKRTSDDKIDMAERDGF 475
Query: 223 TLTMLVPLKAMCKSRPDINNLFK 155
LT L+PLKAMCK+RP +N +FK
Sbjct: 476 VLTKLIPLKAMCKTRPVVNKVFK 498
>ref|XP_016191270.1| isoflavone 2'-hydroxylase [Arachis ipaensis]
Length = 499
Score = 124 bits (312), Expect = 7e-31
Identities = 59/83 (71%), Positives = 69/83 (83%)
Frame = -3
Query: 403 RFEKEGEIDKLIAFGLGRRACPGGALAMRAISLTLGLLVQCFDWKLVDDKEIDMREESGF 224
RFE++GE++KLIAFGLGRRACPG LA+RAISLT+ LLVQCFDWK V D++IDM E GF
Sbjct: 416 RFERKGELEKLIAFGLGRRACPGEGLALRAISLTIALLVQCFDWKCVGDEKIDMTETDGF 475
Query: 223 TLTMLVPLKAMCKSRPDINNLFK 155
TLT VPL A+CK+RP IN L K
Sbjct: 476 TLTKKVPLVALCKTRPVINQLIK 498