BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Astragalus23_contig00004021
         (1442 letters)

Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects 
           149,584,005 sequences; 54,822,741,787 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AFA89978.1| isoflavone-3'-hydroxylase [Astragalus membranaceu...   739   0.0  
ref|XP_004487887.1| PREDICTED: isoflavone 3'-hydroxylase-like [C...   620   0.0  
sp|Q6WNQ9.1|C81E9_MEDTR RecName: Full=Isoflavone 3'-hydroxylase;...   610   0.0  
ref|XP_013463333.1| cytochrome P450 family 81 protein [Medicago ...   610   0.0  
ref|XP_004487888.1| PREDICTED: isoflavone 3'-hydroxylase-like [C...   589   0.0  
ref|XP_015959793.1| isoflavone 3'-hydroxylase [Arachis duranensis]    572   0.0  
gb|AAO31712.1| cytochrome P450 [Pisum sativum]                        569   0.0  
ref|XP_016197998.1| isoflavone 3'-hydroxylase [Arachis ipaensis]      562   0.0  
ref|XP_020224833.1| LOW QUALITY PROTEIN: isoflavone 3'-hydroxyla...   554   0.0  
gb|KYP60203.1| Isoflavone 2'-hydroxylase [Cajanus cajan]              519   e-178
gb|ABD17289.1| isoflavone 3'-hydroxylase, partial [Astragalus mo...   508   e-178
ref|XP_007158858.1| hypothetical protein PHAVU_002G187800g [Phas...   508   e-174
gb|AFC88269.1| isoflavone 3'-hydroxylase 1, partial [Astragalus ...   496   e-173
ref|XP_020223955.1| isoflavone 3'-hydroxylase-like [Cajanus cajan]    506   e-173
ref|XP_017405622.1| PREDICTED: isoflavone 3'-hydroxylase-like [V...   503   e-172
ref|XP_014506448.1| isoflavone 3'-hydroxylase [Vigna radiata var...   503   e-172
ref|XP_003532671.1| PREDICTED: isoflavone 3'-hydroxylase-like [G...   493   e-168
ref|XP_003548043.3| PREDICTED: cytochrome P450 81E8 [Glycine max...   492   e-168
dbj|BAT21113.1| cytochrome P450 [synthetic construct]                 486   e-166
dbj|BAA74465.1| cytochrome P450 [Glycyrrhiza echinata]                486   e-165

>gb|AFA89978.1| isoflavone-3'-hydroxylase [Astragalus membranaceus]
 gb|ATY39977.1| Isoflavone 3'-hydroxylase [Astragalus membranaceus]
          Length = 505

 Score =  739 bits (1907), Expect = 0.0
 Identities = 377/425 (88%), Positives = 377/425 (88%)
 Frame = +2

Query: 167  MAPXXXXXXXXXAFILTVKIILQIQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLS 346
            MAP         AFILTVKIILQIQSRRLKNL           NLHHLKHPLHRTFTTLS
Sbjct: 1    MAPLLYYSLLSLAFILTVKIILQIQSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLS 60

Query: 347  QKYGDIISLWFGSRLVVVVASPSIAQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASY 526
            QKYGDIISLWFGSRLVVVVASPSIAQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASY
Sbjct: 61   QKYGDIISLWFGSRLVVVVASPSIAQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASY 120

Query: 527  GDHWRNLRRITTIDVLSNHRLNSFHGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRL 706
            GDHWRNLRRITTIDVLSNHRLNSFHGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRL
Sbjct: 121  GDHWRNLRRITTIDVLSNHRLNSFHGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRL 180

Query: 707  TEMTFNAMMRMISGKRYYGDDGDVTDVKEAKQFRDIISEILSLLGANNKGXXXXXXXXXX 886
            TEMTFNAMMRMISGKRYYGDDGDVTDVKEAKQFRDIISEILSLLGANNKG          
Sbjct: 181  TEMTFNAMMRMISGKRYYGDDGDVTDVKEAKQFRDIISEILSLLGANNKGDFLPLLRLFD 240

Query: 887  XXXXEKRCKRISKRADAFLQGLIEQHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIK 1066
                EKRCKRISKRADAFLQGLIEQHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIK
Sbjct: 241  LDHLEKRCKRISKRADAFLQGLIEQHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIK 300

Query: 1067 GLIQAMLLAGTDTSAVTIEWVMAELLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYL 1246
            GLIQAMLLAGTDTSAVTIEWVMAELLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYL
Sbjct: 301  GLIQAMLLAGTDTSAVTIEWVMAELLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYL 360

Query: 1247 QNVISEALRXXXXXXXXXXXXXXEDCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFK 1426
            QNVISEALR              EDCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFK
Sbjct: 361  QNVISEALRLHPPAPLLLPHSASEDCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFK 420

Query: 1427 PERFE 1441
            PERFE
Sbjct: 421  PERFE 425


>ref|XP_004487887.1| PREDICTED: isoflavone 3'-hydroxylase-like [Cicer arietinum]
          Length = 500

 Score =  620 bits (1598), Expect = 0.0
 Identities = 311/425 (73%), Positives = 347/425 (81%)
 Frame = +2

Query: 167  MAPXXXXXXXXXAFILTVKIILQIQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLS 346
            M P          FI+T+KIILQIQSR+LKNL           NLHHLKHP+HRTFTTLS
Sbjct: 1    MTPLLYYSLLSITFIITIKIILQIQSRKLKNLPPGPPTIPIIGNLHHLKHPIHRTFTTLS 60

Query: 347  QKYGDIISLWFGSRLVVVVASPSIAQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASY 526
            QKYGD++SLWFGSRLVVVV+SPS+  ECFTKND++LANRP+FLTGKYIF NYTTLGSASY
Sbjct: 61   QKYGDVLSLWFGSRLVVVVSSPSLVNECFTKNDIILANRPQFLTGKYIFNNYTTLGSASY 120

Query: 527  GDHWRNLRRITTIDVLSNHRLNSFHGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRL 706
            GDHWRNLRRITTIDVLSN+RLNSF GVR DET RLV+KL  + + EG   FT VELRHRL
Sbjct: 121  GDHWRNLRRITTIDVLSNNRLNSFLGVRTDETNRLVQKLLKNTISEG---FTKVELRHRL 177

Query: 707  TEMTFNAMMRMISGKRYYGDDGDVTDVKEAKQFRDIISEILSLLGANNKGXXXXXXXXXX 886
            TEMTFN MMRMISGKRYYGDDGDVTDV+EAKQFRDIISEI+SLLGANNKG          
Sbjct: 178  TEMTFNGMMRMISGKRYYGDDGDVTDVEEAKQFRDIISEIMSLLGANNKGDFLPLLRLFD 237

Query: 887  XXXXEKRCKRISKRADAFLQGLIEQHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIK 1066
                EKRCKRI+KRADAFLQGLIE+ Q+ N   NDGDTMIDHLLKLRE+QPEYYSDHMIK
Sbjct: 238  LDDLEKRCKRIAKRADAFLQGLIEEIQSGN--QNDGDTMIDHLLKLRESQPEYYSDHMIK 295

Query: 1067 GLIQAMLLAGTDTSAVTIEWVMAELLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYL 1246
            GLIQAMLLAGTDTSAV IEWVM+ELLN+P+VL KAK+EIET IG+++L+EE DLS+LPYL
Sbjct: 296  GLIQAMLLAGTDTSAVAIEWVMSELLNHPDVLNKAKEEIETQIGRDKLIEEHDLSKLPYL 355

Query: 1247 QNVISEALRXXXXXXXXXXXXXXEDCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFK 1426
            QN+I+E LR              ED T+GGFNVPKDTIIL+N+WAIHRDP LW+DP SFK
Sbjct: 356  QNIINETLRLHPPAPLLLPHYSSEDFTLGGFNVPKDTIILSNVWAIHRDPNLWSDPLSFK 415

Query: 1427 PERFE 1441
            PERFE
Sbjct: 416  PERFE 420


>sp|Q6WNQ9.1|C81E9_MEDTR RecName: Full=Isoflavone 3'-hydroxylase; AltName: Full=Cytochrome
            P450 81E9
 gb|AAQ20041.1| isoflavone 3'-hydroxylase, partial [Medicago truncatula]
          Length = 501

 Score =  610 bits (1573), Expect = 0.0
 Identities = 307/414 (74%), Positives = 345/414 (83%), Gaps = 1/414 (0%)
 Frame = +2

Query: 203  AFILTVKIILQIQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLSQKYGDIISLWFG 382
            +FI+T+KI+L+I SRRLKNL           NLHHLKHPLHRTFTTLSQ YGDI SLWFG
Sbjct: 11   SFIITIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYGDIFSLWFG 70

Query: 383  SRLVVVVASPSIAQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNLRRITT 562
            SRLVVVV+SPS+A ECFTKND++LANRP+FLTGKYIFYNYTTLGSASYGDHWRNLRRITT
Sbjct: 71   SRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLRRITT 130

Query: 563  IDVLSNHRLNSFHGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRLTEMTFNAMMRMI 742
            IDVLSN+RLNSF GVR+DET RL++KL  DVV EG F FT VELR RLTEMTFNAMMRMI
Sbjct: 131  IDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEG-FGFTKVELRPRLTEMTFNAMMRMI 189

Query: 743  SGKRYYGDDGDVTDVKEAKQFRDIISEILSLLGANNKGXXXXXXXXXXXXXXEKRCKRIS 922
            SGKRYYGDDGDV+DV+EAKQFR+IISE++SLLGANNKG              EKRCKRI+
Sbjct: 190  SGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKRCKRIA 249

Query: 923  KRADAFLQGLIEQHQNAN-HSDNDGDTMIDHLLKLRETQPEYYSDHMIKGLIQAMLLAGT 1099
            KR++AFL+GLIE+H+  N HS  DG TMIDHLLKL E+QPEYYSDH+IKGLIQ MLLAGT
Sbjct: 250  KRSNAFLEGLIEEHRRGNIHS--DGGTMIDHLLKLSESQPEYYSDHLIKGLIQGMLLAGT 307

Query: 1100 DTSAVTIEWVMAELLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYLQNVISEALRXX 1279
            DTSAVTIEWVM+ELLN+PEVLKKAK+E++T IGK +LV+EQDLS+LPYLQN+ISE LR  
Sbjct: 308  DTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIISETLRLH 367

Query: 1280 XXXXXXXXXXXXEDCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFKPERFE 1441
                        EDCTIG FNVPKDTIILTN+W IHRDP+ W D  SFKPERFE
Sbjct: 368  PPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERFE 421


>ref|XP_013463333.1| cytochrome P450 family 81 protein [Medicago truncatula]
 gb|KEH37344.1| cytochrome P450 family 81 protein [Medicago truncatula]
          Length = 503

 Score =  610 bits (1573), Expect = 0.0
 Identities = 307/414 (74%), Positives = 345/414 (83%), Gaps = 1/414 (0%)
 Frame = +2

Query: 203  AFILTVKIILQIQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLSQKYGDIISLWFG 382
            +FI+T+KI+L+I SRRLKNL           NLHHLKHPLHRTFTTLSQ YGDI SLWFG
Sbjct: 13   SFIITIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYGDIFSLWFG 72

Query: 383  SRLVVVVASPSIAQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNLRRITT 562
            SRLVVVV+SPS+A ECFTKND++LANRP+FLTGKYIFYNYTTLGSASYGDHWRNLRRITT
Sbjct: 73   SRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLRRITT 132

Query: 563  IDVLSNHRLNSFHGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRLTEMTFNAMMRMI 742
            IDVLSN+RLNSF GVR+DET RL++KL  DVV EG F FT VELR RLTEMTFNAMMRMI
Sbjct: 133  IDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEG-FGFTKVELRPRLTEMTFNAMMRMI 191

Query: 743  SGKRYYGDDGDVTDVKEAKQFRDIISEILSLLGANNKGXXXXXXXXXXXXXXEKRCKRIS 922
            SGKRYYGDDGDV+DV+EAKQFR+IISE++SLLGANNKG              EKRCKRI+
Sbjct: 192  SGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRLFDLDNLEKRCKRIA 251

Query: 923  KRADAFLQGLIEQHQNAN-HSDNDGDTMIDHLLKLRETQPEYYSDHMIKGLIQAMLLAGT 1099
            KR++AFL+GLIE+H+  N HS  DG TMIDHLLKL E+QPEYYSDH+IKGLIQ MLLAGT
Sbjct: 252  KRSNAFLEGLIEEHRRGNIHS--DGGTMIDHLLKLSESQPEYYSDHLIKGLIQGMLLAGT 309

Query: 1100 DTSAVTIEWVMAELLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYLQNVISEALRXX 1279
            DTSAVTIEWVM+ELLN+PEVLKKAK+E++T IGK +LV+EQDLS+LPYLQN+ISE LR  
Sbjct: 310  DTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIISETLRLH 369

Query: 1280 XXXXXXXXXXXXEDCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFKPERFE 1441
                        EDCTIG FNVPKDTIILTN+W IHRDP+ W D  SFKPERFE
Sbjct: 370  PPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERFE 423


>ref|XP_004487888.1| PREDICTED: isoflavone 3'-hydroxylase-like [Cicer arietinum]
          Length = 500

 Score =  589 bits (1518), Expect = 0.0
 Identities = 299/425 (70%), Positives = 338/425 (79%)
 Frame = +2

Query: 167  MAPXXXXXXXXXAFILTVKIILQIQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLS 346
            M P          FI+T+KII+QIQSR+LKNL           NLH LKHP+H TFTTLS
Sbjct: 1    MTPLLYYSLLSITFIITIKIIIQIQSRKLKNLPPGPPTIPIIGNLHQLKHPMHHTFTTLS 60

Query: 347  QKYGDIISLWFGSRLVVVVASPSIAQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASY 526
            QKYGD++SLWFGSRLVVVV+SPS+ QECF KND+VLANRP+ L GKYIFYNYTTLGS+SY
Sbjct: 61   QKYGDVLSLWFGSRLVVVVSSPSLVQECFNKNDIVLANRPQLLAGKYIFYNYTTLGSSSY 120

Query: 527  GDHWRNLRRITTIDVLSNHRLNSFHGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRL 706
            GDHWRNLRRIT IDVLSN+RLNSF GVR DET RL++KL ND V +    FT VELRHRL
Sbjct: 121  GDHWRNLRRITAIDVLSNNRLNSFLGVRTDETNRLIQKLLNDTVSDQ--GFTKVELRHRL 178

Query: 707  TEMTFNAMMRMISGKRYYGDDGDVTDVKEAKQFRDIISEILSLLGANNKGXXXXXXXXXX 886
            TEMTFN MM MISGKRYYGDD DVTDV+EAKQFRDIISEI+SLLGA NK           
Sbjct: 179  TEMTFNGMMMMISGKRYYGDDRDVTDVEEAKQFRDIISEIMSLLGA-NKSEFLPFLRLFD 237

Query: 887  XXXXEKRCKRISKRADAFLQGLIEQHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIK 1066
                 KR K+I+KRADAFLQGLIE+HQ+ N S  +GDTMIDHLL LR++QPEYYSDHMIK
Sbjct: 238  LDDLTKRYKKIAKRADAFLQGLIEEHQSGNQS--NGDTMIDHLLNLRKSQPEYYSDHMIK 295

Query: 1067 GLIQAMLLAGTDTSAVTIEWVMAELLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYL 1246
            GLIQAMLLAGTDTSAVTIEWVM+ELLN+P+VL KAK+EIET IGK++L+EE DLS+LPYL
Sbjct: 296  GLIQAMLLAGTDTSAVTIEWVMSELLNHPDVLNKAKEEIETQIGKDKLIEEHDLSKLPYL 355

Query: 1247 QNVISEALRXXXXXXXXXXXXXXEDCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFK 1426
            QN+I+E LR              ED T+GGFNVPKDTIILTN+WAIHRDP+ W+D SSFK
Sbjct: 356  QNIINETLRLHPPAPLSLPHYSSEDFTLGGFNVPKDTIILTNVWAIHRDPKYWSDASSFK 415

Query: 1427 PERFE 1441
            PERFE
Sbjct: 416  PERFE 420


>ref|XP_015959793.1| isoflavone 3'-hydroxylase [Arachis duranensis]
          Length = 504

 Score =  572 bits (1473), Expect = 0.0
 Identities = 287/413 (69%), Positives = 330/413 (79%)
 Frame = +2

Query: 203  AFILTVKIILQIQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLSQKYGDIISLWFG 382
            AFI+T+K+ L++QSRR KNL           NLH+LK PLHR F  L+  YGDI+SLWFG
Sbjct: 14   AFIITLKLFLRVQSRRFKNLPPGPPTLPFLGNLHYLKPPLHRIFAGLAASYGDIMSLWFG 73

Query: 383  SRLVVVVASPSIAQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNLRRITT 562
            +RLVVVV+SPS+A ECFTKNDVVLANRP+FLTGKYIFYNYTTLGSASYGDHWRNLRRITT
Sbjct: 74   NRLVVVVSSPSLAHECFTKNDVVLANRPRFLTGKYIFYNYTTLGSASYGDHWRNLRRITT 133

Query: 563  IDVLSNHRLNSFHGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRLTEMTFNAMMRMI 742
            +DVLS+HRLNSF  VRKDET RL+EKL  D     N  F  VELR RLTEMTFN MMRMI
Sbjct: 134  VDVLSSHRLNSFLDVRKDETKRLIEKLWKDT---RNGEFVKVELRSRLTEMTFNGMMRMI 190

Query: 743  SGKRYYGDDGDVTDVKEAKQFRDIISEILSLLGANNKGXXXXXXXXXXXXXXEKRCKRIS 922
            SGKRYYGDD +++DV+EAKQFR+II+EILSLLGANNKG              EKR K I+
Sbjct: 191  SGKRYYGDDVEMSDVEEAKQFREIITEILSLLGANNKGDFMPLAKFFDFDNLEKRLKNIA 250

Query: 923  KRADAFLQGLIEQHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIKGLIQAMLLAGTD 1102
            KRAD+FLQGLI++H+ A  S+ D DTMI HLLKL+ETQPEYYSD MIKGLIQAMLLAGTD
Sbjct: 251  KRADSFLQGLIQEHRVAAGSNADSDTMIFHLLKLQETQPEYYSDLMIKGLIQAMLLAGTD 310

Query: 1103 TSAVTIEWVMAELLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYLQNVISEALRXXX 1282
            TSAV +EWVMAELLN+PE++KK K E++T IGK+RLVEEQDLS+LPYLQNVISE+LR   
Sbjct: 311  TSAVAMEWVMAELLNHPEIMKKVKDELDTQIGKDRLVEEQDLSKLPYLQNVISESLRLHP 370

Query: 1283 XXXXXXXXXXXEDCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFKPERFE 1441
                       EDCTIGG+N+PKDTI+LTN+W IHRDP +W D  SF+PERFE
Sbjct: 371  PAPLLLPHSASEDCTIGGYNIPKDTIVLTNVWLIHRDPNIWADAESFRPERFE 423


>gb|AAO31712.1| cytochrome P450 [Pisum sativum]
          Length = 502

 Score =  569 bits (1466), Expect = 0.0
 Identities = 288/426 (67%), Positives = 332/426 (77%), Gaps = 1/426 (0%)
 Frame = +2

Query: 167  MAPXXXXXXXXXAFILTVKIILQIQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLS 346
            M+P          FI+T+KI+LQ QSRRLKNL           NLHHLKHPLHRTF TLS
Sbjct: 1    MSPLFYYSFLSLTFIITIKILLQTQSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFATLS 60

Query: 347  QKYGDIISLWFGSRLVVVVASPSIAQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASY 526
            QKYGDI+SLWFGSRLVVV++S S+ +ECF KNDVVLANRP+FLTGKYIFY+YTTLGS SY
Sbjct: 61   QKYGDIVSLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSY 120

Query: 527  GDHWRNLRRITTIDVLSNHRLNSFHGVRKDETLRLVEKLRNDVVKEG-NFSFTDVELRHR 703
            GDHWRNLRRITTIDVLSN+RL SF GVR DE  RL++    +++K G +  F  VELR  
Sbjct: 121  GDHWRNLRRITTIDVLSNNRLTSFLGVRTDEANRLIQ----NIIKHGASRDFIKVELRSI 176

Query: 704  LTEMTFNAMMRMISGKRYYGDDGDVTDVKEAKQFRDIISEILSLLGANNKGXXXXXXXXX 883
            LTEMTFN MMRMISGKRYYGDDGDVT+V+EAKQFR+IISEILSLLGANNKG         
Sbjct: 177  LTEMTFNGMMRMISGKRYYGDDGDVTEVEEAKQFREIISEILSLLGANNKGDFLPLLRWF 236

Query: 884  XXXXXEKRCKRISKRADAFLQGLIEQHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMI 1063
                 EKRCK+I+ RADAFL+GLI++H++ANH   +GDTMIDHLLKL E Q      H+I
Sbjct: 237  DLDDLEKRCKKIANRADAFLEGLIQEHRSANH--GNGDTMIDHLLKLSEVQLNIIPSHII 294

Query: 1064 KGLIQAMLLAGTDTSAVTIEWVMAELLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPY 1243
            KGLIQAMLLAGTDTSA+ IEWVM+ELLN+PEVL KAK+EIET +GK ++++EQDL +LPY
Sbjct: 295  KGLIQAMLLAGTDTSALAIEWVMSELLNHPEVLNKAKEEIETQVGKNKIIDEQDLPKLPY 354

Query: 1244 LQNVISEALRXXXXXXXXXXXXXXEDCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSF 1423
            LQN+ISEAL+              EDCTIGGFNVPKDTIILTN+WAIHRDP  W+D  SF
Sbjct: 355  LQNIISEALKLHPPAPLLLPHYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSF 414

Query: 1424 KPERFE 1441
            KPERFE
Sbjct: 415  KPERFE 420


>ref|XP_016197998.1| isoflavone 3'-hydroxylase [Arachis ipaensis]
          Length = 529

 Score =  562 bits (1449), Expect = 0.0
 Identities = 282/413 (68%), Positives = 325/413 (78%)
 Frame = +2

Query: 203  AFILTVKIILQIQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLSQKYGDIISLWFG 382
            +FI+T+K+  + QSRR KNL           NLH+LK P+HR F  L+  YGDI+SLWFG
Sbjct: 39   SFIITLKLFFRFQSRRFKNLPPGPPTLPFLGNLHYLKPPVHRIFAGLAASYGDIMSLWFG 98

Query: 383  SRLVVVVASPSIAQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNLRRITT 562
            +RLVVVV+SPS+A ECFTKNDVVLANRP+FLTGKYIFYNYTTLGSASYGDHWRNLRRITT
Sbjct: 99   NRLVVVVSSPSLAHECFTKNDVVLANRPRFLTGKYIFYNYTTLGSASYGDHWRNLRRITT 158

Query: 563  IDVLSNHRLNSFHGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRLTEMTFNAMMRMI 742
            +DVLS+HRLNSF  VRKDET RL+EKL  D     N  F  VELR RLTEMTFN MMRMI
Sbjct: 159  VDVLSSHRLNSFLDVRKDETKRLIEKLWKDT---RNGEFVKVELRSRLTEMTFNGMMRMI 215

Query: 743  SGKRYYGDDGDVTDVKEAKQFRDIISEILSLLGANNKGXXXXXXXXXXXXXXEKRCKRIS 922
            SGKRYYGDD ++ DV+EAKQFR+II+EILSLLGA+NKG              EKR K I+
Sbjct: 216  SGKRYYGDDVEMNDVEEAKQFREIITEILSLLGASNKGDFMPLAKFFDFDHLEKRLKNIA 275

Query: 923  KRADAFLQGLIEQHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIKGLIQAMLLAGTD 1102
            KRAD+FLQGLI++H+ A  S+ D DTMI HLLKL+ETQPEYYSD MIKGLIQAMLLAGTD
Sbjct: 276  KRADSFLQGLIQEHRVAAGSNADSDTMIFHLLKLQETQPEYYSDLMIKGLIQAMLLAGTD 335

Query: 1103 TSAVTIEWVMAELLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYLQNVISEALRXXX 1282
            TSAV +EW MAELLN+PE++KK K E++T IGK+RLVEEQDLS+LPYLQNVISE LR   
Sbjct: 336  TSAVAMEWAMAELLNHPEIMKKVKDELDTQIGKDRLVEEQDLSKLPYLQNVISETLRLHP 395

Query: 1283 XXXXXXXXXXXEDCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFKPERFE 1441
                       EDCTIGG+N+PKDTI+LTN+W IHRDP +W D  SF+PERFE
Sbjct: 396  PAPLLLPHSASEDCTIGGYNIPKDTIVLTNVWLIHRDPNIWADAESFRPERFE 448


>ref|XP_020224833.1| LOW QUALITY PROTEIN: isoflavone 3'-hydroxylase-like [Cajanus cajan]
          Length = 500

 Score =  554 bits (1427), Expect = 0.0
 Identities = 285/425 (67%), Positives = 322/425 (75%)
 Frame = +2

Query: 167  MAPXXXXXXXXXAFILTVKIILQIQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLS 346
            MAP         AF LT+KI+LQ QSRR KNL           NLH LK P+HR+ + L 
Sbjct: 1    MAPFLYYSLLFLAFTLTIKILLQAQSRRPKNLPPGPPPFPLIGNLHRLKPPIHRSISALC 60

Query: 347  QKYGDIISLWFGSRLVVVVASPSIAQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASY 526
            + YGDI+SLWFGSRLVVVV+SPS+ QECF +NDVVLANRP+FL GKYIFYN     SASY
Sbjct: 61   RTYGDIVSLWFGSRLVVVVSSPSLVQECFGRNDVVLANRPRFLAGKYIFYNXR---SASY 117

Query: 527  GDHWRNLRRITTIDVLSNHRLNSFHGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRL 706
            GDHWRNLRRITTIDVLS HRLNSF G+R+DE  RLV +L  D       +   V LR RL
Sbjct: 118  GDHWRNLRRITTIDVLSTHRLNSFVGIRRDEARRLVARLGQDATCREGEALKKVALRPRL 177

Query: 707  TEMTFNAMMRMISGKRYYGDDGDVTDVKEAKQFRDIISEILSLLGANNKGXXXXXXXXXX 886
            TEMTFNAMMRMISGKRYYGDD DVTD +EAKQFR+IISE+LSLLGANNKG          
Sbjct: 178  TEMTFNAMMRMISGKRYYGDDCDVTDAEEAKQFREIISEMLSLLGANNKGDFLPLLRLFD 237

Query: 887  XXXXEKRCKRISKRADAFLQGLIEQHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIK 1066
                EKR KRI+KRADAFLQ LIE+H++  H +   DTMIDHLLKL E+QPEYYSDHMIK
Sbjct: 238  FDDLEKRLKRIAKRADAFLQSLIEEHRSGKHGN--ADTMIDHLLKLGESQPEYYSDHMIK 295

Query: 1067 GLIQAMLLAGTDTSAVTIEWVMAELLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYL 1246
            GLIQAMLLAGTDTSAVTIEWVM+ELLNNPEVLKKAK EI+T IGK+RLVEEQDLS+LPYL
Sbjct: 296  GLIQAMLLAGTDTSAVTIEWVMSELLNNPEVLKKAKDEIDTQIGKDRLVEEQDLSKLPYL 355

Query: 1247 QNVISEALRXXXXXXXXXXXXXXEDCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFK 1426
            QN+ISE LR              EDCTIGG+++P++TI+LTN W IH DP+LW+DP SF 
Sbjct: 356  QNIISETLRLHPPAPLLLPHESSEDCTIGGYHIPRNTIVLTNAWLIHSDPKLWSDPMSFM 415

Query: 1427 PERFE 1441
            PERFE
Sbjct: 416  PERFE 420


>gb|KYP60203.1| Isoflavone 2'-hydroxylase [Cajanus cajan]
          Length = 506

 Score =  519 bits (1337), Expect = e-178
 Identities = 276/443 (62%), Positives = 313/443 (70%), Gaps = 18/443 (4%)
 Frame = +2

Query: 167  MAPXXXXXXXXXAFILTVKIILQIQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLS 346
            MAP         AF LT+KI+LQ QSRR KNL           NLH LK P+HR+ + L 
Sbjct: 1    MAPFLYYSLLFLAFTLTIKILLQAQSRRPKNLPPGPPPFPLIGNLHRLKPPIHRSISALC 60

Query: 347  QKYGDIISLWFGSRLVVVVASPSIAQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASY 526
            + YGDI+SLWFGSRLVVVV+SPS+ QECF +NDVVLANRP+               SASY
Sbjct: 61   RTYGDIVSLWFGSRLVVVVSSPSLVQECFGRNDVVLANRPR---------------SASY 105

Query: 527  GDHWRNLRRITTIDVLSNHRLNSFHGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRL 706
            GDHWRNLRRITTIDVLS HRLNSF G+R+DE  RLV +L  D       +   V LR RL
Sbjct: 106  GDHWRNLRRITTIDVLSTHRLNSFVGIRRDEARRLVARLGQDATCREGEALKKVALRPRL 165

Query: 707  TEMTFNAMMRMISGKRYYGDDGDVTDVKEAKQFRDIISEILSLLGANNKGXXXXXXXXXX 886
            TEMTFNAMMRMISGKRYYGDD DVTD +EAKQFR+IISE+LSLLGANNKG          
Sbjct: 166  TEMTFNAMMRMISGKRYYGDDCDVTDAEEAKQFREIISEMLSLLGANNKGDFLPLLRLFD 225

Query: 887  XXXXEKRCKRISKRADAFLQGLIEQHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIK 1066
                EKR KRI+KRADAFLQ LIE+H++  H   + DTMIDHLLKL E+QPEYYSDHMIK
Sbjct: 226  FDDLEKRLKRIAKRADAFLQSLIEEHRSGKH--GNADTMIDHLLKLGESQPEYYSDHMIK 283

Query: 1067 GLI------------------QAMLLAGTDTSAVTIEWVMAELLNNPEVLKKAKKEIETT 1192
            GLI                  QAMLLAGTDTSAVTIEWVM+ELLNNPEVLKKAK EI+T 
Sbjct: 284  GLIQSVFPELSFGLHYECAFRQAMLLAGTDTSAVTIEWVMSELLNNPEVLKKAKDEIDTQ 343

Query: 1193 IGKERLVEEQDLSQLPYLQNVISEALRXXXXXXXXXXXXXXEDCTIGGFNVPKDTIILTN 1372
            IGK+RLVEEQDLS+LPYLQN+ISE LR              EDCTIGG+++P++TI+LTN
Sbjct: 344  IGKDRLVEEQDLSKLPYLQNIISETLRLHPPAPLLLPHESSEDCTIGGYHIPRNTIVLTN 403

Query: 1373 IWAIHRDPELWTDPSSFKPERFE 1441
             W IH DP+LW+DP SF PERFE
Sbjct: 404  AWLIHSDPKLWSDPMSFMPERFE 426


>gb|ABD17289.1| isoflavone 3'-hydroxylase, partial [Astragalus mongholicus]
          Length = 268

 Score =  508 bits (1308), Expect = e-178
 Identities = 253/267 (94%), Positives = 253/267 (94%)
 Frame = +2

Query: 296  NLHHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSIAQECFTKNDVVLANRPKFL 475
            NLHHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSIAQECFTKNDVVLANRPKFL
Sbjct: 2    NLHHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSIAQECFTKNDVVLANRPKFL 61

Query: 476  TGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNSFHGVRKDETLRLVEKLRNDV 655
            TGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNSFHGVRKDETLRLVEKLRNDV
Sbjct: 62   TGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNSFHGVRKDETLRLVEKLRNDV 121

Query: 656  VKEGNFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGDVTDVKEAKQFRDIISEILSL 835
            VKEGNFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGDVTDVKEAKQFRDIISEILSL
Sbjct: 122  VKEGNFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGDVTDVKEAKQFRDIISEILSL 181

Query: 836  LGANNKGXXXXXXXXXXXXXXEKRCKRISKRADAFLQGLIEQHQNANHSDNDGDTMIDHL 1015
            LGANNKG              EKRCKRISKRADAFLQGLIEQHQNANHSDNDGDTMIDHL
Sbjct: 182  LGANNKGDFLPLLRLFDLDHLEKRCKRISKRADAFLQGLIEQHQNANHSDNDGDTMIDHL 241

Query: 1016 LKLRETQPEYYSDHMIKGLIQAMLLAG 1096
            LKLRETQPEYYSDHMIKGLIQAMLLAG
Sbjct: 242  LKLRETQPEYYSDHMIKGLIQAMLLAG 268


>ref|XP_007158858.1| hypothetical protein PHAVU_002G187800g [Phaseolus vulgaris]
 gb|ESW30852.1| hypothetical protein PHAVU_002G187800g [Phaseolus vulgaris]
          Length = 500

 Score =  508 bits (1307), Expect = e-174
 Identities = 255/425 (60%), Positives = 308/425 (72%)
 Frame = +2

Query: 167  MAPXXXXXXXXXAFILTVKIILQIQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLS 346
            M P         A I T+K +LQ   RRLKNL           NLH +K PLHR+F +LS
Sbjct: 1    MVPLVYYSLIFLASIFTLKFLLQ--RRRLKNLPPHPPTLPIIGNLHLIKPPLHRSFLSLS 58

Query: 347  QKYGDIISLWFGSRLVVVVASPSIAQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASY 526
            QKYG I SLWFGSR VVV+ SP++ QECFTK D VLANRP+ LTGKY+FYNYT++GS+ Y
Sbjct: 59   QKYGPIFSLWFGSRYVVVITSPTLVQECFTKYDTVLANRPRLLTGKYLFYNYTSMGSSPY 118

Query: 527  GDHWRNLRRITTIDVLSNHRLNSFHGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRL 706
            G+HWRNLRRI TIDVLS  RLNSF  +R++ET+R+++KL  +  K     FT V++R RL
Sbjct: 119  GEHWRNLRRIITIDVLSTQRLNSFFEIRREETMRVIQKLVRETCK----GFTHVQIRPRL 174

Query: 707  TEMTFNAMMRMISGKRYYGDDGDVTDVKEAKQFRDIISEILSLLGANNKGXXXXXXXXXX 886
            TEMTFN MMRMISGKRYYGDDGDVTD +EAKQFRDIISE++SLLGANNKG          
Sbjct: 175  TEMTFNNMMRMISGKRYYGDDGDVTDAEEAKQFRDIISEVMSLLGANNKGDFLPLLRWFD 234

Query: 887  XXXXEKRCKRISKRADAFLQGLIEQHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIK 1066
                EKR K IS+RADAFLQGL+E+H++  H  N   TMI+HLL ++E+QPEYYSDH+IK
Sbjct: 235  FDGLEKRLKSISRRADAFLQGLLEEHRSGKHKAN---TMIEHLLTMQESQPEYYSDHIIK 291

Query: 1067 GLIQAMLLAGTDTSAVTIEWVMAELLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYL 1246
            GLIQ MLLAGTDT+AVT+EW +A LLN PE+LKKA+ EI+  +G++RLVEE D+  LPYL
Sbjct: 292  GLIQGMLLAGTDTTAVTMEWAVASLLNEPEILKKAQDEIKKCVGEDRLVEESDIPNLPYL 351

Query: 1247 QNVISEALRXXXXXXXXXXXXXXEDCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFK 1426
            QN+I E LR              + CTIGGF +P  TI+L N WAI RDPE W+D + FK
Sbjct: 352  QNIIYETLRLFSPAPLLLPHYSSDHCTIGGFTIPPHTIVLINAWAIQRDPETWSDATCFK 411

Query: 1427 PERFE 1441
            PERFE
Sbjct: 412  PERFE 416


>gb|AFC88269.1| isoflavone 3'-hydroxylase 1, partial [Astragalus membranaceus]
          Length = 262

 Score =  496 bits (1277), Expect = e-173
 Identities = 247/262 (94%), Positives = 247/262 (94%)
 Frame = +2

Query: 302  HHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSIAQECFTKNDVVLANRPKFLTG 481
            HHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSIAQECFTKNDVVLANRPKFLTG
Sbjct: 1    HHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPSIAQECFTKNDVVLANRPKFLTG 60

Query: 482  KYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNSFHGVRKDETLRLVEKLRNDVVK 661
            KYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNSFHGVRKDETLRLVEKLRNDVVK
Sbjct: 61   KYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNSFHGVRKDETLRLVEKLRNDVVK 120

Query: 662  EGNFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGDVTDVKEAKQFRDIISEILSLLG 841
            EGNFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGDVTDVKEAKQFR IISEILSLLG
Sbjct: 121  EGNFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGDVTDVKEAKQFRGIISEILSLLG 180

Query: 842  ANNKGXXXXXXXXXXXXXXEKRCKRISKRADAFLQGLIEQHQNANHSDNDGDTMIDHLLK 1021
            ANNKG              EKRCKRISKRADAFLQGLIEQHQNANHSDNDGDTMIDHLLK
Sbjct: 181  ANNKGDFLPLLRLFDLDHLEKRCKRISKRADAFLQGLIEQHQNANHSDNDGDTMIDHLLK 240

Query: 1022 LRETQPEYYSDHMIKGLIQAML 1087
            LRETQPEYYSDHMIKGLIQAML
Sbjct: 241  LRETQPEYYSDHMIKGLIQAML 262


>ref|XP_020223955.1| isoflavone 3'-hydroxylase-like [Cajanus cajan]
          Length = 537

 Score =  506 bits (1302), Expect = e-173
 Identities = 260/414 (62%), Positives = 302/414 (72%), Gaps = 1/414 (0%)
 Frame = +2

Query: 203  AFILTV-KIILQIQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLSQKYGDIISLWF 379
            AFI T+ KI+LQ QSRR +NL           NLHHLK P H  F  LSQ YGD+ISL F
Sbjct: 42   AFIFTLLKILLQTQSRRFRNLPPGPPTLPIIGNLHHLKPPNHHAFAVLSQTYGDVISLRF 101

Query: 380  GSRLVVVVASPSIAQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNLRRIT 559
            G RLVVV++S S+ Q+CF  NDVVLANRP+FL GKY+ Y+YTTLGS SYG HWRNLRRI 
Sbjct: 102  GRRLVVVLSSLSVVQQCFAANDVVLANRPRFLVGKYVSYDYTTLGSTSYGHHWRNLRRIA 161

Query: 560  TIDVLSNHRLNSFHGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRLTEMTFNAMMRM 739
            T+DVLS HRLNSF GVR+DE  RLV KL  D        F  VELR RLTEMTFN MMRM
Sbjct: 162  TVDVLSTHRLNSFVGVRRDEIRRLVMKLGQDATCREGEGFAKVELRWRLTEMTFNNMMRM 221

Query: 740  ISGKRYYGDDGDVTDVKEAKQFRDIISEILSLLGANNKGXXXXXXXXXXXXXXEKRCKRI 919
            I+G+R YG D DV + + A+QFR++I+E++SL GANNKG              EKR KRI
Sbjct: 222  ITGRRCYGQDCDVKEAETARQFREVITEMVSLSGANNKGDFLPLLRLFDFEDLEKRLKRI 281

Query: 920  SKRADAFLQGLIEQHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIKGLIQAMLLAGT 1099
            ++RADAFL  LIE+H++  H     D MIDHLLKL + QPEYYSDH+IKGLIQAMLLAGT
Sbjct: 282  AERADAFLHSLIEEHRSGKH--GSVDNMIDHLLKLCQLQPEYYSDHIIKGLIQAMLLAGT 339

Query: 1100 DTSAVTIEWVMAELLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYLQNVISEALRXX 1279
            DT A+TIEWVM+ELLNNPEVLKKAK EI T IGK+RLVEEQDL +L YLQN+ISE  R  
Sbjct: 340  DTPALTIEWVMSELLNNPEVLKKAKDEINTHIGKDRLVEEQDLPKLSYLQNIISETFRLH 399

Query: 1280 XXXXXXXXXXXXEDCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFKPERFE 1441
                        EDCTIGG+++P+ TI+LTN W IHRDP LW+DP+SFKPERFE
Sbjct: 400  PLAPLLLPHESSEDCTIGGYHIPRGTIVLTNAWLIHRDPILWSDPTSFKPERFE 453


>ref|XP_017405622.1| PREDICTED: isoflavone 3'-hydroxylase-like [Vigna angularis]
 gb|KOM25512.1| hypothetical protein LR48_Vigan107s003100 [Vigna angularis]
 dbj|BAT74197.1| hypothetical protein VIGAN_01181600 [Vigna angularis var. angularis]
          Length = 500

 Score =  503 bits (1295), Expect = e-172
 Identities = 253/424 (59%), Positives = 309/424 (72%)
 Frame = +2

Query: 167  MAPXXXXXXXXXAFILTVKIILQIQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLS 346
            M P         A I T+K +L+   RRL+NL           NLH +K PLHR+  +LS
Sbjct: 1    MVPLVYYSLLFLASIFTLKFLLR--RRRLENLPPHPPTLPIIGNLHLIKPPLHRSLLSLS 58

Query: 347  QKYGDIISLWFGSRLVVVVASPSIAQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASY 526
            Q+YG I SLWFGSR VVV+ SP++ QECFTK D VLANRP+FLTGKY+FYNYT++GS+ Y
Sbjct: 59   QQYGPIFSLWFGSRFVVVITSPTLVQECFTKYDTVLANRPRFLTGKYLFYNYTSMGSSPY 118

Query: 527  GDHWRNLRRITTIDVLSNHRLNSFHGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRL 706
            GDHWRNLRRI TIDVLS  RLNSF  VR++ET+R+V+KL    V+E +  F  V++R RL
Sbjct: 119  GDHWRNLRRIITIDVLSTQRLNSFFEVRREETMRVVQKL----VRETSKGFALVQIRPRL 174

Query: 707  TEMTFNAMMRMISGKRYYGDDGDVTDVKEAKQFRDIISEILSLLGANNKGXXXXXXXXXX 886
            TEMTFN MMRMISGKRYYGDDGDVTD +EAKQFR II+E++SLLGANNKG          
Sbjct: 175  TEMTFNNMMRMISGKRYYGDDGDVTDAEEAKQFRAIITEVMSLLGANNKGDFLPLLRWFD 234

Query: 887  XXXXEKRCKRISKRADAFLQGLIEQHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIK 1066
                EKR K IS RADAFLQGL+E+H++  H  N   TMI+HLL ++ETQPEYYSDH+IK
Sbjct: 235  FDGLEKRLKSISTRADAFLQGLLEEHRSGKHKTN---TMIEHLLTMQETQPEYYSDHIIK 291

Query: 1067 GLIQAMLLAGTDTSAVTIEWVMAELLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYL 1246
            GLIQ MLLAGTDT+AVT+EW MA LLN+PE+LKKA+ EI+  +G++RLV+E D+  LPY+
Sbjct: 292  GLIQGMLLAGTDTTAVTMEWAMASLLNHPEILKKAQDEIDKCVGQDRLVDESDIPNLPYI 351

Query: 1247 QNVISEALRXXXXXXXXXXXXXXEDCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFK 1426
            QN+I E LR              E CT+GGF VP+DTI+L N WAI RDP+ W+D + FK
Sbjct: 352  QNIIYETLRLFSPAPMLLPHYSSEQCTLGGFTVPRDTIVLINAWAIQRDPQTWSDATCFK 411

Query: 1427 PERF 1438
            PERF
Sbjct: 412  PERF 415


>ref|XP_014506448.1| isoflavone 3'-hydroxylase [Vigna radiata var. radiata]
          Length = 500

 Score =  503 bits (1294), Expect = e-172
 Identities = 253/424 (59%), Positives = 308/424 (72%)
 Frame = +2

Query: 167  MAPXXXXXXXXXAFILTVKIILQIQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLS 346
            M P         AFI T K +L+   RRL+NL           NLH +K PLHR+  +LS
Sbjct: 1    MVPLIYYSLLFLAFIFTFKFLLR--RRRLENLPPHPPTLPIIGNLHLIKPPLHRSLLSLS 58

Query: 347  QKYGDIISLWFGSRLVVVVASPSIAQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASY 526
            Q+YG I SLWFGSR VVV+ SP++ QECFTK D+VLANRP+FLTGKY+FYNYT++GS+SY
Sbjct: 59   QQYGPIFSLWFGSRFVVVITSPTLVQECFTKYDIVLANRPRFLTGKYLFYNYTSMGSSSY 118

Query: 527  GDHWRNLRRITTIDVLSNHRLNSFHGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRL 706
            GDHWRNLRRI TIDVLS  RLNSF  VR++ET+R+++KL  +  K     F  V++R RL
Sbjct: 119  GDHWRNLRRIITIDVLSTQRLNSFFEVRREETMRVMQKLVRETCK----GFALVQIRPRL 174

Query: 707  TEMTFNAMMRMISGKRYYGDDGDVTDVKEAKQFRDIISEILSLLGANNKGXXXXXXXXXX 886
            TEMTFN MMRMISGKRYYGDDGDVTD +EAKQFR II+E++SLLGANNKG          
Sbjct: 175  TEMTFNNMMRMISGKRYYGDDGDVTDAEEAKQFRAIITEVMSLLGANNKGDFLPLLRWFD 234

Query: 887  XXXXEKRCKRISKRADAFLQGLIEQHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIK 1066
                EKR K IS RADAFLQGL+E+H++  H  N   TMI+HLL ++ETQPEYYSDH+IK
Sbjct: 235  FDGLEKRLKSISTRADAFLQGLLEEHRSGKHKTN---TMIEHLLTMQETQPEYYSDHIIK 291

Query: 1067 GLIQAMLLAGTDTSAVTIEWVMAELLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYL 1246
            GLIQ MLLAGTDT+AVT+EW MA LLN+PE+LKKA+ EI+  +G++RLV+E D+  LPYL
Sbjct: 292  GLIQGMLLAGTDTTAVTMEWAMASLLNHPEILKKAQDEIDNCVGEDRLVDESDIPNLPYL 351

Query: 1247 QNVISEALRXXXXXXXXXXXXXXEDCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFK 1426
            QN+I E LR              E  T+GGF VP+DTI+L N WAI RDP+ W+D + FK
Sbjct: 352  QNIIYETLRLFSPAPMLLPHYSSEQFTLGGFTVPRDTIVLINAWAIQRDPQTWSDATCFK 411

Query: 1427 PERF 1438
            PERF
Sbjct: 412  PERF 415


>ref|XP_003532671.1| PREDICTED: isoflavone 3'-hydroxylase-like [Glycine max]
 gb|KHN28918.1| Isoflavone 2'-hydroxylase [Glycine soja]
 gb|KRH42430.1| hypothetical protein GLYMA_08G089400 [Glycine max]
          Length = 500

 Score =  493 bits (1270), Expect = e-168
 Identities = 240/413 (58%), Positives = 306/413 (74%)
 Frame = +2

Query: 203  AFILTVKIILQIQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLSQKYGDIISLWFG 382
            + ++ V I+  +Q RR+KNL           NLH++K PLHR+  +LS+KYG I SLWFG
Sbjct: 8    SLLVLVLILRFVQRRRVKNLPPCPPSLPLIGNLHYIKSPLHRSLLSLSEKYGPIFSLWFG 67

Query: 383  SRLVVVVASPSIAQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNLRRITT 562
            SR VVV++SP++ QECFTK+D+VLANRP+FLTGKY+FYNY+++GS+ YGDHWRNLRRI T
Sbjct: 68   SRFVVVISSPTLLQECFTKHDIVLANRPRFLTGKYLFYNYSSMGSSPYGDHWRNLRRIIT 127

Query: 563  IDVLSNHRLNSFHGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRLTEMTFNAMMRMI 742
            IDVLS  RLNSF  +R++ET+R+++KL  +        F  V LR RLTEMTFN MMRMI
Sbjct: 128  IDVLSTSRLNSFFEIRREETMRVIQKLARETCN----GFALVHLRPRLTEMTFNNMMRMI 183

Query: 743  SGKRYYGDDGDVTDVKEAKQFRDIISEILSLLGANNKGXXXXXXXXXXXXXXEKRCKRIS 922
            SGKRYYGDD +  D +EAKQFRDI++E++SLLGANNKG              EKR K IS
Sbjct: 184  SGKRYYGDDIEAADAEEAKQFRDIMTEVMSLLGANNKGDFLPFLRWFDFDGLEKRLKVIS 243

Query: 923  KRADAFLQGLIEQHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIKGLIQAMLLAGTD 1102
             RAD+FLQGL+E+H++  H  N   TMI+HLL ++E+QP YYSDH+IKGLIQ MLLAGTD
Sbjct: 244  TRADSFLQGLLEEHRSGKHKAN---TMIEHLLTMQESQPHYYSDHIIKGLIQGMLLAGTD 300

Query: 1103 TSAVTIEWVMAELLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYLQNVISEALRXXX 1282
            T+AV IEW ++ LLN+PE+LKKAK EI+  +G++RLV+E D+ +LPYLQN+I E LR   
Sbjct: 301  TTAVAIEWAVSSLLNHPEILKKAKDEIDNMVGQDRLVDESDIPKLPYLQNIIYETLRLFA 360

Query: 1283 XXXXXXXXXXXEDCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFKPERFE 1441
                       E+CTIGGF +P+DTI+L N WAI RDPE W+D + FKPERFE
Sbjct: 361  PAPLLLPHYSSEECTIGGFTIPRDTIVLINAWAIQRDPEHWSDATCFKPERFE 413


>ref|XP_003548043.3| PREDICTED: cytochrome P450 81E8 [Glycine max]
 gb|KHN13402.1| Isoflavone 2'-hydroxylase [Glycine soja]
 gb|KRH08433.1| hypothetical protein GLYMA_16G149300 [Glycine max]
          Length = 498

 Score =  492 bits (1266), Expect = e-168
 Identities = 247/413 (59%), Positives = 300/413 (72%)
 Frame = +2

Query: 203  AFILTVKIILQIQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLSQKYGDIISLWFG 382
            AFIL + I   IQ+RR KNL           NLH LK PLHRTF  LSQKYG I SLWFG
Sbjct: 10   AFILFLSINFLIQTRRFKNLPPGPFSFPIIGNLHQLKQPLHRTFHALSQKYGPIFSLWFG 69

Query: 383  SRLVVVVASPSIAQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNLRRITT 562
            SR VVVV+SP   QECFTKND+VLANRP FLTGKYI YN TT+  + YGDHWRNLRRI  
Sbjct: 70   SRFVVVVSSPLAVQECFTKNDIVLANRPHFLTGKYIGYNNTTVAVSPYGDHWRNLRRIMA 129

Query: 563  IDVLSNHRLNSFHGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRLTEMTFNAMMRMI 742
            ++VLS HR+NSF   R+DE +RLV+KL  D        FT VEL+ R +EMTFN +MRM+
Sbjct: 130  LEVLSTHRINSFLENRRDEIMRLVQKLARD----SRNGFTKVELKSRFSEMTFNTIMRMV 185

Query: 743  SGKRYYGDDGDVTDVKEAKQFRDIISEILSLLGANNKGXXXXXXXXXXXXXXEKRCKRIS 922
            SGKRYYG+D DV+DV+EA+QFR+II E+++L GANN G              EKR KRIS
Sbjct: 186  SGKRYYGEDCDVSDVQEARQFREIIKELVTLGGANNPGDFLALLRWFDFDGLEKRLKRIS 245

Query: 923  KRADAFLQGLIEQHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIKGLIQAMLLAGTD 1102
            KR DAFLQGLI+QH+N  H  N   TMIDHLL  +++QPEYY+D +IKGL   MLLAGTD
Sbjct: 246  KRTDAFLQGLIDQHRNGKHRAN---TMIDHLLAQQQSQPEYYTDQIIKGLALVMLLAGTD 302

Query: 1103 TSAVTIEWVMAELLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYLQNVISEALRXXX 1282
            TSAVT+EW M+ LLN+PE+LKKAK E++T IG++RLV+E D+ +LPYLQ+++ E LR   
Sbjct: 303  TSAVTLEWAMSNLLNHPEILKKAKNELDTHIGQDRLVDEPDIPKLPYLQSIVYETLRLHP 362

Query: 1283 XXXXXXXXXXXEDCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFKPERFE 1441
                       EDCTIG +N+P++TI+L N WAIHRDP+LW+DP+ FKPERFE
Sbjct: 363  AAPMLVPHLSSEDCTIGEYNIPQNTILLVNAWAIHRDPKLWSDPTHFKPERFE 415


>dbj|BAT21113.1| cytochrome P450 [synthetic construct]
          Length = 477

 Score =  486 bits (1250), Expect = e-166
 Identities = 240/402 (59%), Positives = 300/402 (74%)
 Frame = +2

Query: 236  IQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPS 415
            I++R+ KNL           NLHHLK PLHRTF  LS+KYG + SLWFGSRLVVVV+S S
Sbjct: 2    IRARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHVFSLWFGSRLVVVVSSAS 61

Query: 416  IAQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNS 595
              Q+CFTKNDVVLANRP+FL+GKYIFYNYTTLGS SYG+HWRNLRRIT +DVLSNHR+NS
Sbjct: 62   EFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITALDVLSNHRINS 121

Query: 596  FHGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGD 775
            F G+R+DET RL+ +L +D     + +F ++EL  RL +MTFN +MRMISGKRYYG+D D
Sbjct: 122  FSGIRRDETQRLITRLADD----SSTNFAEMELSSRLYDMTFNNIMRMISGKRYYGEDCD 177

Query: 776  VTDVKEAKQFRDIISEILSLLGANNKGXXXXXXXXXXXXXXEKRCKRISKRADAFLQGLI 955
             +D++EA QFRD++SE+L L GANNK               EKR K IS + DAFL+GLI
Sbjct: 178  TSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKDISGKTDAFLRGLI 237

Query: 956  EQHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIKGLIQAMLLAGTDTSAVTIEWVMA 1135
            E+H+      N   TMIDHLL L+++QPEYY+D +IKGL  AMLLAGTD+SAVT+EW M+
Sbjct: 238  EEHRTKKERAN---TMIDHLLNLQDSQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMS 294

Query: 1136 ELLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYLQNVISEALRXXXXXXXXXXXXXX 1315
             LLN+PEVLKK K E++T +G++RLV+E DL +L YL+NVI+E LR              
Sbjct: 295  NLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINETLRLYTPAPLLLPHSTS 354

Query: 1316 EDCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFKPERFE 1441
            ++C IGG+ VP+DTI+L N WAIHRDPELWT+ ++FKPERFE
Sbjct: 355  DECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFE 396


>dbj|BAA74465.1| cytochrome P450 [Glycyrrhiza echinata]
          Length = 499

 Score =  486 bits (1251), Expect = e-165
 Identities = 239/402 (59%), Positives = 302/402 (75%)
 Frame = +2

Query: 236  IQSRRLKNLXXXXXXXXXXXNLHHLKHPLHRTFTTLSQKYGDIISLWFGSRLVVVVASPS 415
            I++R+ KNL           NLHHLK PLHRTF  LS+KYG +ISLWFGSRLVVVV+S S
Sbjct: 24   IRARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHVISLWFGSRLVVVVSSAS 83

Query: 416  IAQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNLRRITTIDVLSNHRLNS 595
              Q+CFTKNDVVLANRP+FL+GKYIFYNYTTLGS SYG+HWRNLRRIT +DVLSNHR+NS
Sbjct: 84   EFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRRITALDVLSNHRINS 143

Query: 596  FHGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRLTEMTFNAMMRMISGKRYYGDDGD 775
            F G+R+DET RL+ +L +D     + +F ++EL +R  +MTFN +MRMISGKRYYG+D D
Sbjct: 144  FSGIRRDETQRLITRLADD----SSTNFAEIELSYRFYDMTFNNIMRMISGKRYYGEDCD 199

Query: 776  VTDVKEAKQFRDIISEILSLLGANNKGXXXXXXXXXXXXXXEKRCKRISKRADAFLQGLI 955
            ++D++EA QFRD++SE+L L GANNK               EKR K IS + DAFL+GLI
Sbjct: 200  MSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKDISGKTDAFLRGLI 259

Query: 956  EQHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIKGLIQAMLLAGTDTSAVTIEWVMA 1135
            ++H+      N   TMIDHLL L+++QPEYY+D +IKGL  AMLLAGTD+SAVT+EW M+
Sbjct: 260  QEHRAKKERAN---TMIDHLLNLQDSQPEYYTDQIIKGLALAMLLAGTDSSAVTLEWSMS 316

Query: 1136 ELLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYLQNVISEALRXXXXXXXXXXXXXX 1315
             LLN+PEVLKK K E++T +G++RLV+E DL +L YL+NVI+E LR              
Sbjct: 317  NLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLSYLKNVINETLRLYTPAPLLLPHSTS 376

Query: 1316 EDCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFKPERFE 1441
            ++C IGG+ VP+DTI+L N WAIHRDPELWT+ ++FKPERFE
Sbjct: 377  DECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFE 418