BLASTX nr result
ID: Astragalus23_contig00000566
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Astragalus23_contig00000566
(1929 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
149,584,005 sequences; 54,822,741,787 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_015952953.1| DNA topoisomerase 2-like [Arachis duranensis] 267 1e-79
ref|XP_016188028.1| DNA topoisomerase 2-like [Arachis ipaensis] 266 3e-79
gb|PNY03875.1| DNA topoisomerase 2-like protein [Trifolium prate... 265 1e-78
ref|XP_003599271.2| DNA topoisomerase IV subunit A [Medicago tru... 261 3e-77
gb|PNY06816.1| DNA topoisomerase 2-like protein [Trifolium prate... 261 6e-77
dbj|GAU12625.1| hypothetical protein TSUD_121160 [Trifolium subt... 259 1e-76
ref|XP_023527820.1| DNA topoisomerase 2 isoform X1 [Cucurbita pe... 166 3e-76
ref|XP_023527823.1| DNA topoisomerase 2 isoform X3 [Cucurbita pe... 166 3e-76
ref|XP_023527825.1| DNA topoisomerase 2 isoform X5 [Cucurbita pe... 166 3e-76
ref|XP_023527826.1| DNA topoisomerase 2 isoform X6 [Cucurbita pe... 166 3e-76
ref|XP_022935015.1| DNA topoisomerase 2 isoform X1 [Cucurbita mo... 166 4e-76
ref|XP_022982562.1| DNA topoisomerase 2 isoform X1 [Cucurbita ma... 166 4e-76
ref|XP_022935016.1| DNA topoisomerase 2 isoform X2 [Cucurbita mo... 166 4e-76
ref|XP_022982563.1| DNA topoisomerase 2 isoform X2 [Cucurbita ma... 166 4e-76
ref|XP_020212372.1| DNA topoisomerase 2 [Cajanus cajan] 251 3e-75
ref|XP_004499912.1| PREDICTED: DNA topoisomerase 2 [Cicer arieti... 253 1e-74
ref|XP_022152631.1| DNA topoisomerase 2 isoform X1 [Momordica ch... 164 9e-73
ref|XP_022152632.1| DNA topoisomerase 2 isoform X2 [Momordica ch... 164 9e-73
ref|XP_019454379.1| PREDICTED: DNA topoisomerase 2 [Lupinus angu... 241 2e-72
ref|XP_003601827.1| DNA topoisomerase IV subunit A [Medicago tru... 241 6e-69
>ref|XP_015952953.1| DNA topoisomerase 2-like [Arachis duranensis]
Length = 1483
Score = 267 bits (682), Expect(2) = 1e-79
Identities = 207/589 (35%), Positives = 290/589 (49%), Gaps = 32/589 (5%)
Frame = -3
Query: 1777 SFKRALSEEISLTALIGYVSEHADYHHSGETLGSIIIRMAQNYVSSNNINIFEPIGQFGT 1598
SFK+ L++EI + IGYVSE + YHH ++L S II MAQ++V SNNIN+ +P GQFGT
Sbjct: 724 SFKKKLTKEIKVAQFIGYVSEQSAYHHGEQSLASTIIGMAQDFVGSNNINLLKPNGQFGT 783
Query: 1597 GNLGGQDHAP--QLKTKLNPLSRLLFPRIDDEVLRYFNRGGRSKEPTWYIPIRKWCQRDG 1424
NLGG+DHA + T+LNP++R LFP DD ++ Y N G+S EP WYIP+ +G
Sbjct: 784 RNLGGKDHASARYIYTELNPITRRLFPEDDDNLVDYLNEDGKSIEPNWYIPVIPLVLVNG 843
Query: 1423 N-WI*FFYSKL**TFFYSKL**T----------LTMDPCYXXXXXXXXXXXXXGSYGVRG 1277
+ I +S + ++ + MDP Y G Y V G
Sbjct: 844 SEGIGVGWSSYIPNYNPREIIANVRRMMNGETMIPMDPWYRGYKGTIEKSAKEGGYIVNG 903
Query: 1276 KVEEINNETLRVTELPVGKWTEPYKKFLNSITAGSHADGTDHMLMARILSLRTSHNIKMI 1097
VEEIN +T R+TELP+ KWT+ YK+FL S+T DG+ + I R + + I
Sbjct: 904 MVEEINEQTFRITELPIRKWTQDYKQFLESLT-----DGSPTVKDPLIEDFRQNGDDATI 958
Query: 1096 KRQI*KSR*SNKKALIMGENLDRAYQKLNLITRISTSNMNLFDADGRMKTFVSLEQSKAL 917
++ K ++M E L +K L + ISTSNM+LFDA+G++K + + EQ
Sbjct: 959 DIEVRMK--LEKVPMVMQEGL---LKKFKLSSSISTSNMHLFDAEGKIKKYDNPEQILEE 1013
Query: 916 FCRVVLKNNNINSVFYVLIILYHFLCSSERVLCTTAALL*KTKGKYTAQPFPTYSDVGEH 737
F + L+ +YV Y L + ERVL T + G +
Sbjct: 1014 FFPLRLE-------YYVRRKKYK-LDNLERVLLTLDNKVRFILGVVNGE----------- 1054
Query: 736 S*VSRNGFGRRYYYLTWLNRSKFELISELKHQGFDPKPRKGKSAEPRVAGANDDNSEVQE 557
+ NR K +L+ ELK +GF P PRKGKSAEP+VAG NDDN E QE
Sbjct: 1055 --------------IIVSNRRKADLLIELKQKGFTPMPRKGKSAEPQVAGDNDDNPEEQE 1100
Query: 556 -----------DDYEYLTSIPLSFFSLNHLDKLLAEKMKKMCELNVLKEISCSEMWMMDL 410
DYEYL S+P+ +L + KLLAEK +K E +LK MWM DL
Sbjct: 1101 TESVNVEGARVGDYEYLLSMPIGTLTLESVQKLLAEKDEKEKEYEILKATPPKSMWMEDL 1160
Query: 409 VELEKKIDEINRXXXXXXXXXXXXXXENVVEVTKPKGSAGSKKTQKDSADAAIITSLKRC 230
+LEKK+DE+ E + + S +KKT S A + K+
Sbjct: 1161 DQLEKKLDELE---------------TKEAEEERKRSSQANKKT---STRAVTTKASKKP 1202
Query: 229 LDEYYQSSS-----SSEITGGDMETEVA---ATMKRRVVSLAEALNITD 107
+ + ++ + +I+ ME E A A K RV S E + + D
Sbjct: 1203 RQKNIKKANVIEEEAEDISNSSMERENAPEVAKPKGRVGSKKEPIKMVD 1251
Score = 60.8 bits (146), Expect(2) = 1e-79
Identities = 29/49 (59%), Positives = 37/49 (75%)
Frame = -1
Query: 1926 GNSRDDGNRRISYEEFIFQYTIEFSVADLRRSIPSMVDGLKPSQRKVLY 1780
G RD + ISY +F+ + I FS ADL+RSIPSM+DGLKPSQRK+L+
Sbjct: 674 GTCRDHRAKHISYRDFVDKELILFSRADLQRSIPSMIDGLKPSQRKILF 722
>ref|XP_016188028.1| DNA topoisomerase 2-like [Arachis ipaensis]
Length = 1486
Score = 266 bits (679), Expect(2) = 3e-79
Identities = 208/589 (35%), Positives = 292/589 (49%), Gaps = 32/589 (5%)
Frame = -3
Query: 1777 SFKRALSEEISLTALIGYVSEHADYHHSGETLGSIIIRMAQNYVSSNNINIFEPIGQFGT 1598
SFK+ L++EI + IGYVSE + YHH ++L S II MAQ++V SNNIN+ +P GQFGT
Sbjct: 724 SFKKKLTKEIKVAQFIGYVSEQSAYHHGEQSLASTIIGMAQDFVGSNNINLLKPNGQFGT 783
Query: 1597 GNLGGQDHAP--QLKTKLNPLSRLLFPRIDDEVLRYFNRGGRSKEPTWYIPIRKWCQRDG 1424
NLGG+DHA + T+LNP++R LFP DD ++ Y N G+S EP WYIP+ +G
Sbjct: 784 RNLGGKDHASARYIYTELNPITRRLFPEDDDNLVDYLNEDGKSIEPNWYIPVIPLVLVNG 843
Query: 1423 N------WI*FF--YSKL**TFFYSKL---**TLTMDPCYXXXXXXXXXXXXXGSYGVRG 1277
+ W + Y+ ++ + MDP Y G Y V G
Sbjct: 844 SEGIGVGWSSYIPNYNPRDIIANVRRMMNGETMIPMDPWYRGYKGTIEKSAKEGGYIVNG 903
Query: 1276 KVEEINNETLRVTELPVGKWTEPYKKFLNSITAGSHADGTDHMLMARILSLRTSHNIKMI 1097
VEEIN +T R+TELP+ KWT+ YK+FL S+T DG+ + I R + + I
Sbjct: 904 MVEEINEQTFRITELPIRKWTQDYKQFLESLT-----DGSPTVKDPLIEDFRQNGDDATI 958
Query: 1096 KRQI*KSR*SNKKALIMGENLDRAYQKLNLITRISTSNMNLFDADGRMKTFVSLEQSKAL 917
++ K ++M E L +K L + ISTSNM+LFDA+G++K + + EQ
Sbjct: 959 DIEVRMK--LEKVPMVMQEGL---LKKFKLSSSISTSNMHLFDAEGKIKKYDNPEQILEE 1013
Query: 916 FCRVVLKNNNINSVFYVLIILYHFLCSSERVLCTTAALL*KTKGKYTAQPFPTYSDVGEH 737
F + L+ +YV Y L + ERVL L+ K ++
Sbjct: 1014 FFPLRLE-------YYVRRKKYK-LDNLERVL-----LILDNKVRFI------------- 1047
Query: 736 S*VSRNGFGRRYYYLTWLNRSKFELISELKHQGFDPKPRKGKSAEPRVAGANDDNSEVQE 557
G + NR K +L+ ELK +GF P PRKGKSAEP+VAG NDDN E QE
Sbjct: 1048 -------LGVVNGEIIVSNRRKADLLIELKQKGFTPMPRKGKSAEPQVAGDNDDNPEEQE 1100
Query: 556 -----------DDYEYLTSIPLSFFSLNHLDKLLAEKMKKMCELNVLKEISCSEMWMMDL 410
DYEYL S+P+ +L + KLLAEK +K E +LK MWM DL
Sbjct: 1101 TESFNVEGARVGDYEYLLSMPIGSLTLESVQKLLAEKDEKEKEYEILKATPPKSMWMEDL 1160
Query: 409 VELEKKIDEINRXXXXXXXXXXXXXXENVVEVTKPKGSAGSKKTQKDSADAAIITSLKRC 230
+LEKK+DE+ E + + S +KKT S A + K+
Sbjct: 1161 DQLEKKLDELE---------------TKEAEEERKRSSQANKKT---STRAVTTKASKKP 1202
Query: 229 LDEYYQSSS-----SSEITGGDMETEVA---ATMKRRVVSLAEALNITD 107
+ + ++ + +I+ ME E A A K RV S E + + D
Sbjct: 1203 RQKNIKKANVIEEETEDISNSSMERENAPEVAKPKGRVGSKKEPIKVVD 1251
Score = 60.8 bits (146), Expect(2) = 3e-79
Identities = 29/49 (59%), Positives = 37/49 (75%)
Frame = -1
Query: 1926 GNSRDDGNRRISYEEFIFQYTIEFSVADLRRSIPSMVDGLKPSQRKVLY 1780
G RD + ISY +F+ + I FS ADL+RSIPSM+DGLKPSQRK+L+
Sbjct: 674 GTCRDHRAKHISYRDFVDKELILFSRADLQRSIPSMIDGLKPSQRKILF 722
>gb|PNY03875.1| DNA topoisomerase 2-like protein [Trifolium pratense]
Length = 1492
Score = 265 bits (676), Expect(2) = 1e-78
Identities = 188/496 (37%), Positives = 260/496 (52%), Gaps = 29/496 (5%)
Frame = -3
Query: 1777 SFKRALSEEISLTALIGYVSEHADYHHSGETLGSIIIRMAQNYVSSNNINIFEPIGQFGT 1598
SFK+ L +EI + IGYVSEH+ YHH ++L S II MAQ++V SNNIN+ +P GQFGT
Sbjct: 708 SFKKKLVKEIKVGQFIGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLKPNGQFGT 767
Query: 1597 GNLGGQDHAPQ--LKTKLNPLSRLLFPRIDDEVLRYFNRGGRSKEPTWYIPIRKWCQRDG 1424
NLGG+DHA + T+LN ++R LF DD++L Y N GRS EPTWYIPI +G
Sbjct: 768 RNLGGKDHASSRYIYTELNTVTRSLFHEDDDKLLEYLNEDGRSIEPTWYIPIIPLVLVNG 827
Query: 1423 N------WI*FF--YSKL**TFFYSKL---**TLTMDPCYXXXXXXXXXXXXXGSYGVRG 1277
+ W + Y+ + +L + MDP Y G Y V G
Sbjct: 828 SEGIGTGWSSYIPNYNPREISANVRRLLNGEEMVPMDPWYRGFRGTIEKSAKEGGYIVNG 887
Query: 1276 KVEEINNETLRVTELPVGKWTEPYKKFLNSITAGSHADGTDHMLMARILSLRTSHNIKMI 1097
V EI+ +T R+TELP+ KWT+ YK+FL SIT DGT ++ I R + + +++
Sbjct: 888 SVVEIDEQTFRITELPIRKWTQDYKQFLESIT-----DGTPNVKDPLIEDFRQNGDDEIV 942
Query: 1096 KRQI*KSR*SNKKALIMGENLDRAYQKLNLITRISTSNMNLFDADGRMKTFVSLEQSKAL 917
++ ++K A IM E L ++K L ISTSNM+LFDA+G +K F + EQ
Sbjct: 943 DIEV--KMKADKIATIMQEGL---FKKFKLTGTISTSNMHLFDAEGNIKKFDNPEQILEE 997
Query: 916 FCRVVLKNNNINSVFYVLIILYHFLCSSERVLCTTAALL*KTKGKYTAQPFPTYSDVGEH 737
F + L+ Y + L + ER+L L+ K ++
Sbjct: 998 FFPLRLE--------YYEKRKKYILANLERLL-----LILDNKVRFI------------- 1031
Query: 736 S*VSRNGFGRRYYYLTWLNRSKFELISELKHQGFDPKPRKGKSAEPRVAGANDDNSEVQE 557
G + NR K EL+ ELK +GF P PRKGKS+EP+VAGANDDNSE +E
Sbjct: 1032 -------LGVVNGEIIVSNRKKAELLKELKQKGFTPMPRKGKSSEPQVAGANDDNSEERE 1084
Query: 556 D----------------DYEYLTSIPLSFFSLNHLDKLLAEKMKKMCELNVLKEISCSEM 425
D DYEYL S+ + +L + KLL EK +K E +L +
Sbjct: 1085 DGEQETGTVRVEEATWGDYEYLLSLSIGTLTLESVQKLLDEKAEKEKEFEILMGTPSKSL 1144
Query: 424 WMMDLVELEKKIDEIN 377
W+ DL E EKK+DE++
Sbjct: 1145 WLKDLDEFEKKLDELD 1160
Score = 59.7 bits (143), Expect(2) = 1e-78
Identities = 28/49 (57%), Positives = 38/49 (77%)
Frame = -1
Query: 1926 GNSRDDGNRRISYEEFIFQYTIEFSVADLRRSIPSMVDGLKPSQRKVLY 1780
G RD + I+Y++F+ + I FS+ADL+RSIPSMVDGLKP QRK+L+
Sbjct: 658 GTCRDHEAKLINYKDFVNKELILFSMADLQRSIPSMVDGLKPGQRKILF 706
>ref|XP_003599271.2| DNA topoisomerase IV subunit A [Medicago truncatula]
gb|AES69522.2| DNA topoisomerase IV subunit A [Medicago truncatula]
Length = 1504
Score = 261 bits (666), Expect(2) = 3e-77
Identities = 213/625 (34%), Positives = 291/625 (46%), Gaps = 79/625 (12%)
Frame = -3
Query: 1777 SFKRALSEEISLTALIGYVSEHADYHHSGETLGSIIIRMAQNYVSSNNINIFEPIGQFGT 1598
SFK+ LS+E+ + IGYVSEH+ YHH ++L S II MAQ++V SNNIN+ P GQFGT
Sbjct: 709 SFKKNLSKEVKVGQFIGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLTPNGQFGT 768
Query: 1597 GNLGGQDHAPQ--LKTKLNPLSRLLFPRIDDEVLRYFNRGGRSKEPTWYIPIRKWCQRDG 1424
NLGG+DHA + T+LN ++R LF DD++L Y N GRS EP WYIPI +G
Sbjct: 769 RNLGGKDHASSRYIYTQLNTVTRSLFHADDDKLLEYLNEDGRSIEPNWYIPIIPLVLVNG 828
Query: 1423 N------WI*FF--YSKL**TFFYSKL---**TLTMDPCYXXXXXXXXXXXXXGSYGVRG 1277
+ W F Y+ +L + MDP Y G Y V G
Sbjct: 829 SEGIGTGWSSFIPNYNPREIIANVRRLLNDEEMVPMDPWYKGFRGTIEKSAKEGGYIVNG 888
Query: 1276 KVEEINNETLRVTELPVGKWTEPYKKFLNSITAGSHADGTDHMLMARILSLRTSHNIKMI 1097
VEEI++ T R+TELP+ KWT+ YK FL T +G ++ I + + + ++
Sbjct: 889 SVEEIDDITFRITELPIRKWTQDYKTFLELTT-----EGAPNVKDPLIEDFKQNGDDAIV 943
Query: 1096 KRQI*KSR*SNKKALIMGENLDRAYQKLNLITRISTSNMNLFDADGRMKTFVSLEQSKAL 917
++ + K A IM E L ++K L + ISTSNM+LFDA+G++K F + EQ
Sbjct: 944 DIEVRMK--AEKVAAIMQEGL---FKKFKLTSTISTSNMHLFDAEGKIKKFDTPEQ---- 994
Query: 916 FCRVVLKNNNINSVFYVLIILY------HFLCSSERVLCTTAALL*KTKGKYTAQPFPTY 755
I FY L + Y + L + ER+L L+ K ++
Sbjct: 995 ----------ILEEFYPLRLEYYEKRKKYILANLERLL-----LILDNKVRFI------- 1032
Query: 754 SDVGEHS*VSRNGFGRRYYYLTWLNRSKFELISELKHQGFDPKPRKGKSAEPRVAGANDD 575
G + NR + EL+ ELK +GF P P+KGKSAEP+VAGAND+
Sbjct: 1033 -------------LGVVSGEIIVSNRKRTELLIELKQKGFTPIPKKGKSAEPQVAGANDE 1079
Query: 574 NSEVQED------------------DYEYLTSIPLSFFSLNHLDKLLAEKMKKMCELNVL 449
NSE QED DYEYL S+ + +L + KLL EK K E +L
Sbjct: 1080 NSEDQEDSERETASKSVNVEGATWGDYEYLLSLSIGTLTLESVQKLLDEKADKENEFEIL 1139
Query: 448 KEISCSEMWMMDLVELEKKIDEINR----------------------------------- 374
+W+ DL ELEKK+DE++R
Sbjct: 1140 MGTPSKSLWLKDLDELEKKLDELDRIEAEEGQKRSTMSNKKNRLATKPAKKPPQPRKNTK 1199
Query: 373 -XXXXXXXXXXXXXXENVVE------VTKPKGSAGSKKTQKDSADAAIITSLKRCLDEYY 215
EN VE V KPKG AGSKK + AD I+ SL+ L Y
Sbjct: 1200 KANVEPENDNSSMEIENAVEVVKPVEVAKPKGRAGSKKNAQTEADDEIL-SLQERLAVYN 1258
Query: 214 QSSSSSEITGGDMETEVAATMKRRV 140
SS + + E V K+++
Sbjct: 1259 LESSGEKSQAMEEEPPVPPAGKKQI 1283
Score = 59.3 bits (142), Expect(2) = 3e-77
Identities = 29/49 (59%), Positives = 37/49 (75%)
Frame = -1
Query: 1926 GNSRDDGNRRISYEEFIFQYTIEFSVADLRRSIPSMVDGLKPSQRKVLY 1780
G RD + ISY++F+ + I FS ADL+RSIPSMVDGLKP QRK+L+
Sbjct: 659 GTCRDHEAKLISYKDFVDKELILFSRADLQRSIPSMVDGLKPGQRKILF 707
>gb|PNY06816.1| DNA topoisomerase 2-like protein [Trifolium pratense]
Length = 1254
Score = 261 bits (668), Expect(2) = 6e-77
Identities = 214/612 (34%), Positives = 288/612 (47%), Gaps = 75/612 (12%)
Frame = -3
Query: 1777 SFKRALSEEISLTALIGYVSEHADYHHSGETLGSIIIRMAQNYVSSNNINIFEPIGQFGT 1598
SFK+ L +EI + IGYVSEH+ YHH ++L S II MAQ++V SNNIN+ +P GQFGT
Sbjct: 524 SFKKKLVKEIKVGQFIGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLKPNGQFGT 583
Query: 1597 GNLGGQDHAPQ--LKTKLNPLSRLLFPRIDDEVLRYFNRGGRSKEPTWYIPIRKWCQRDG 1424
NLGG+DHA + T+LN ++R LF DD++L Y N GRS EP WYIPI +G
Sbjct: 584 RNLGGKDHASSRYIYTELNTVTRCLFHEDDDKLLEYLNEEGRSIEPNWYIPIIPLVLVNG 643
Query: 1423 N------WI*FF--YSKL**TFFYSKL---**TLTMDPCYXXXXXXXXXXXXXGSYGVRG 1277
+ W + Y+ +L + MDP Y G Y V G
Sbjct: 644 SEGIGTGWSSYIPNYNPREIIANVRRLLNEEEMVPMDPWYRGFRGTIEKSAKEGGYIVSG 703
Query: 1276 KVEEINNETLRVTELPVGKWTEPYKKFLNSITAGSHADGTDHMLMARILSLRTSHNIKMI 1097
V EI+ +T R+TELPV KWT+ YK+FL SIT DGT ++ I R + + ++
Sbjct: 704 SVVEIDEQTFRITELPVRKWTQDYKQFLESIT-----DGTPNVKDPLIEDFRQNGDDAIV 758
Query: 1096 KRQI*KSR*SNKKALIMGENLDRAYQKLNLITRISTSNMNLFDADGRMKTFVSLEQSKAL 917
++ K A IM E L ++K L ISTSNM+LFDA+G++K F + EQ
Sbjct: 759 DIEV--KMKPEKIASIMQEGL---FKKFKLTNTISTSNMHLFDAEGKIKKFDTPEQ---- 809
Query: 916 FCRVVLKNNNINSVFYVLIILY------HFLCSSERVLCTTAALL*KTKGKYTAQPFPTY 755
I FY L + Y + L + ER+L L+ K ++
Sbjct: 810 ----------ILEEFYPLRMEYYERRKKYILANLERLL-----LILDNKVRFI------- 847
Query: 754 SDVGEHS*VSRNGFGRRYYYLTWLNRSKFELISELKHQGFDPKPRKGKSAEPRVAGANDD 575
G + NR K EL+ ELK +GF P RKGKSAEP+VAGANDD
Sbjct: 848 -------------LGVVNGEIIVSNRKKAELLIELKQKGFTPM-RKGKSAEPQVAGANDD 893
Query: 574 NSEVQED-----------------DYEYLTSIPLSFFSLNHLDKLLAEKMKKMCELNVLK 446
NSE QED DYEYL S+ + +L + KLL EK +K E +L
Sbjct: 894 NSEGQEDEQETVSRPVSVEGATWGDYEYLLSLSIGTLTLESVQKLLDEKAEKEKEYEILM 953
Query: 445 EISCSEMWMMDLVELEKKIDEIN---------------------------------RXXX 365
+W+ DL + EKK+DE++ R
Sbjct: 954 GTPTKSLWLKDLDDFEKKLDELDSKEAEDDRKRSSQASKKTANGFAAKPAKKPPQPRKNT 1013
Query: 364 XXXXXXXXXXXENVVEV------TKPKGSAGSKKTQKDSADAAIITSLKRCLDEYYQSSS 203
+ +EV KPKG GSK TQK + D + I SL+ L Y SS
Sbjct: 1014 KKNKDVEPENDNSSMEVENAVEVVKPKGRVGSKNTQKKAGDESDIPSLQERLAAYNFESS 1073
Query: 202 SSEITGGDMETE 167
+ G ME++
Sbjct: 1074 GEK--SGAMESD 1083
Score = 57.4 bits (137), Expect(2) = 6e-77
Identities = 28/49 (57%), Positives = 36/49 (73%)
Frame = -1
Query: 1926 GNSRDDGNRRISYEEFIFQYTIEFSVADLRRSIPSMVDGLKPSQRKVLY 1780
G RD + I+Y+ F+ + I FS ADL+RSIPSMVDGLKP QRK+L+
Sbjct: 474 GTCRDHEAKLINYKNFVNKELILFSRADLQRSIPSMVDGLKPGQRKILF 522
>dbj|GAU12625.1| hypothetical protein TSUD_121160 [Trifolium subterraneum]
Length = 1315
Score = 259 bits (662), Expect(2) = 1e-76
Identities = 187/504 (37%), Positives = 260/504 (51%), Gaps = 37/504 (7%)
Frame = -3
Query: 1777 SFKRALSEEISLTALIGYVSEHADYHHSGETLGSIIIRMAQNYVSSNNINIFEPIGQFGT 1598
SFK+ L +EI + IGYVSEH+ YHH ++L S II MAQ++V SNNIN+ +P GQFGT
Sbjct: 523 SFKKKLVKEIKVGQFIGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLKPNGQFGT 582
Query: 1597 GNLGGQDHAPQ--LKTKLNPLSRLLFPRIDDEVLRYFNRGGRSKEPTWYIPIRKWCQRDG 1424
NLGG+DHA + T+LN ++R LF DD++L Y N GRS EPTWYIPI +G
Sbjct: 583 RNLGGKDHASSRYIYTELNTVTRCLFHENDDKLLEYLNEEGRSIEPTWYIPIIPLVLVNG 642
Query: 1423 N------WI*FF--YSKL**TFFYSKL---**TLTMDPCYXXXXXXXXXXXXXGSYGVRG 1277
+ W + Y+ +L + MDP Y G Y V G
Sbjct: 643 SEGIGTGWSSYIPNYNPREICANVRRLLNDEEMVPMDPWYRGFRGTIEKSAKEGGYIVNG 702
Query: 1276 KVEEINNETLRVTELPVGKWTEPYKKFLNSITAGSHADGTDHMLMARILSLRTSHNIKMI 1097
V EI+ +T R+TELP+ KWT+ YK+FL SIT DGT ++ I R + + +++
Sbjct: 703 SVVEIDEQTFRITELPIRKWTQDYKQFLESIT-----DGTPNVKDPLIEDFRQNGDDEIV 757
Query: 1096 KRQI*KSR*SNKKALIMGENLDRAYQKLNLITRISTSNMNLFDADGRMKTFVSLEQSKAL 917
++ ++K IM E L ++K L ISTSNM+LFDA+G++K F + EQ
Sbjct: 758 DIEV--KMKADKIDTIMQEGL---FKKFKLTGTISTSNMHLFDAEGKIKKFDNPEQ---- 808
Query: 916 FCRVVLKNNNINSVFYVLIILY------HFLCSSERVLCTTAALL*KTKGKYTAQPFPTY 755
I FY L + Y + L + ER+L L+ K ++
Sbjct: 809 ----------ILEEFYPLRLEYYEKRKKYILANLERLL-----LILDNKVRFI------- 846
Query: 754 SDVGEHS*VSRNGFGRRYYYLTWLNRSKFELISELKHQGFDPKPRKGKSAEPRVAGANDD 575
G + NR K EL+ ELK +GF P PRKGKS+EP+VAGAND+
Sbjct: 847 -------------LGVVNGEIIVSNRKKAELLKELKQKGFTPMPRKGKSSEPQVAGANDE 893
Query: 574 NSEVQED------------------DYEYLTSIPLSFFSLNHLDKLLAEKMKKMCELNVL 449
+SE +ED DYEYL S+ + +L + KLL EK +K E +L
Sbjct: 894 SSEEREDIEQETGSQPVRVEEATWGDYEYLLSLSIGTLTLESVQKLLNEKAEKEKEFEIL 953
Query: 448 KEISCSEMWMMDLVELEKKIDEIN 377
+W+ DL E EKK+DE++
Sbjct: 954 MGTPSKALWLKDLDEFEKKLDELD 977
Score = 58.5 bits (140), Expect(2) = 1e-76
Identities = 28/49 (57%), Positives = 37/49 (75%)
Frame = -1
Query: 1926 GNSRDDGNRRISYEEFIFQYTIEFSVADLRRSIPSMVDGLKPSQRKVLY 1780
G RD + I+Y++F+ + I FS ADL+RSIPSMVDGLKP QRK+L+
Sbjct: 473 GTCRDHDAKLINYKDFVNKELILFSRADLQRSIPSMVDGLKPGQRKILF 521
>ref|XP_023527820.1| DNA topoisomerase 2 isoform X1 [Cucurbita pepo subsp. pepo]
ref|XP_023527822.1| DNA topoisomerase 2 isoform X2 [Cucurbita pepo subsp. pepo]
Length = 1491
Score = 166 bits (419), Expect(4) = 3e-76
Identities = 115/300 (38%), Positives = 159/300 (53%), Gaps = 17/300 (5%)
Frame = -3
Query: 1777 SFKRALSEEISLTALIGYVSEHADYHHSGETLGSIIIRMAQNYVSSNNINIFEPIGQFGT 1598
SFKR +E ++ GYVSEH+ YHH ++L S II MAQ++V SNNIN+ P GQFGT
Sbjct: 719 SFKRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGT 778
Query: 1597 GNLGGQDHAPQ--LKTKLNPLSRLLFPRIDDEVLRYFNRGGRSKEPTWYIPIRKWCQRDG 1424
N GG+DHA + TKL+ +R LFP+ DD++L Y N G+S EPTWY+PI +G
Sbjct: 779 RNHGGKDHASSRYIYTKLSNATRFLFPKDDDKLLNYLNEDGQSIEPTWYMPIIPTVLVNG 838
Query: 1423 N------WI*FF--YSKL**TFFYSKL---**TLTMDPCY---XXXXXXXXXXXXXGSYG 1286
+ W + Y+ ++ L MDP Y SY
Sbjct: 839 SEGIGTGWSSYIPNYNPRDIIRNVRRMLNGEAMLPMDPWYRGFSGTIEKTATKEGGTSYT 898
Query: 1285 VRGKVEEINNETLRVTELPVGKWTEPYKKFLNSITAGSHADGTDHMLMARILSL-RTSHN 1109
V G +EE+++ TLR+TELPV +WT YK FL+S+T S + D A+ + + H+
Sbjct: 899 VSGIIEEVSDTTLRITELPVRRWTNDYKDFLDSVTRVSTKNDKDKEKAAKDPFIEQADHH 958
Query: 1108 IKMIKRQI*KSR*SNKKALIMGENLDRAYQKLNLITRISTSNMNLFDADGRMKTFVSLEQ 929
I L M E L + K L T +STSNM+LFDA+G +K + + EQ
Sbjct: 959 SDDKTVSIDVHLTEENMILAMQEGLKK---KFKLTTTVSTSNMHLFDANGVIKKYDTPEQ 1015
Score = 82.8 bits (203), Expect(4) = 3e-76
Identities = 50/174 (28%), Positives = 89/174 (51%), Gaps = 10/174 (5%)
Frame = -3
Query: 682 NRSKFELISELKHQGFDPKPRKGKSAEPRVAGANDDNSEVQE----------DDYEYLTS 533
NR + +L EL+ +GF P P+K KS EP VAGA+++ E +E DYEYL +
Sbjct: 1065 NRRRVDLFHELQEKGFTPFPKKTKSVEPEVAGASNETDEAEESPEVVKGERVSDYEYLLA 1124
Query: 532 IPLSFFSLNHLDKLLAEKMKKMCELNVLKEISCSEMWMMDLVELEKKIDEINRXXXXXXX 353
+ + ++ + +L E+ K E+ L++ + +W+ DL LEK++DE++R
Sbjct: 1125 LAIGTLTIEKVQELCNERDKFKGEVEELRKATPKSLWLKDLDYLEKELDEMDRNDAQADE 1184
Query: 352 XXXXXXXENVVEVTKPKGSAGSKKTQKDSADAAIITSLKRCLDEYYQSSSSSEI 191
+N KPK S + K ++S + ++ +D SS+++E+
Sbjct: 1185 VRKQQRIKN--NDVKPKISRNAPKAPRNSKKTSNSETITESMDITTSSSTTTEV 1236
Score = 58.2 bits (139), Expect(4) = 3e-76
Identities = 27/49 (55%), Positives = 35/49 (71%)
Frame = -1
Query: 1926 GNSRDDGNRRISYEEFIFQYTIEFSVADLRRSIPSMVDGLKPSQRKVLY 1780
G D + I Y +F+ + I FS+ADL+RSIPSMVDGLKP QRK+L+
Sbjct: 669 GTHLDQSEKLIKYSDFVNKELILFSMADLQRSIPSMVDGLKPGQRKILF 717
Score = 52.0 bits (123), Expect(4) = 3e-76
Identities = 23/47 (48%), Positives = 34/47 (72%)
Frame = -1
Query: 837 VLKEFYALRLRYYEKRKANILLNLSQHILMLENTARYLEMVLEGDII 697
+L+EF+ LRL ++EKRK N+L NL +L LEN R++ V+ G+II
Sbjct: 1016 ILEEFFPLRLEFFEKRKRNLLENLETELLKLENKVRFILAVVNGEII 1062
>ref|XP_023527823.1| DNA topoisomerase 2 isoform X3 [Cucurbita pepo subsp. pepo]
ref|XP_023527824.1| DNA topoisomerase 2 isoform X4 [Cucurbita pepo subsp. pepo]
Length = 1490
Score = 166 bits (419), Expect(4) = 3e-76
Identities = 115/300 (38%), Positives = 159/300 (53%), Gaps = 17/300 (5%)
Frame = -3
Query: 1777 SFKRALSEEISLTALIGYVSEHADYHHSGETLGSIIIRMAQNYVSSNNINIFEPIGQFGT 1598
SFKR +E ++ GYVSEH+ YHH ++L S II MAQ++V SNNIN+ P GQFGT
Sbjct: 719 SFKRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGT 778
Query: 1597 GNLGGQDHAPQ--LKTKLNPLSRLLFPRIDDEVLRYFNRGGRSKEPTWYIPIRKWCQRDG 1424
N GG+DHA + TKL+ +R LFP+ DD++L Y N G+S EPTWY+PI +G
Sbjct: 779 RNHGGKDHASSRYIYTKLSNATRFLFPKDDDKLLNYLNEDGQSIEPTWYMPIIPTVLVNG 838
Query: 1423 N------WI*FF--YSKL**TFFYSKL---**TLTMDPCY---XXXXXXXXXXXXXGSYG 1286
+ W + Y+ ++ L MDP Y SY
Sbjct: 839 SEGIGTGWSSYIPNYNPRDIIRNVRRMLNGEAMLPMDPWYRGFSGTIEKTATKEGGTSYT 898
Query: 1285 VRGKVEEINNETLRVTELPVGKWTEPYKKFLNSITAGSHADGTDHMLMARILSL-RTSHN 1109
V G +EE+++ TLR+TELPV +WT YK FL+S+T S + D A+ + + H+
Sbjct: 899 VSGIIEEVSDTTLRITELPVRRWTNDYKDFLDSVTRVSTKNDKDKEKAAKDPFIEQADHH 958
Query: 1108 IKMIKRQI*KSR*SNKKALIMGENLDRAYQKLNLITRISTSNMNLFDADGRMKTFVSLEQ 929
I L M E L + K L T +STSNM+LFDA+G +K + + EQ
Sbjct: 959 SDDKTVSIDVHLTEENMILAMQEGLKK---KFKLTTTVSTSNMHLFDANGVIKKYDTPEQ 1015
Score = 82.8 bits (203), Expect(4) = 3e-76
Identities = 50/174 (28%), Positives = 89/174 (51%), Gaps = 10/174 (5%)
Frame = -3
Query: 682 NRSKFELISELKHQGFDPKPRKGKSAEPRVAGANDDNSEVQE----------DDYEYLTS 533
NR + +L EL+ +GF P P+K KS EP VAGA+++ E +E DYEYL +
Sbjct: 1065 NRRRVDLFHELQEKGFTPFPKKTKSVEPEVAGASNETDEAEESPEVVKGERVSDYEYLLA 1124
Query: 532 IPLSFFSLNHLDKLLAEKMKKMCELNVLKEISCSEMWMMDLVELEKKIDEINRXXXXXXX 353
+ + ++ + +L E+ K E+ L++ + +W+ DL LEK++DE++R
Sbjct: 1125 LAIGTLTIEKVQELCNERDKFKGEVEELRKATPKSLWLKDLDYLEKELDEMDRNDAQADE 1184
Query: 352 XXXXXXXENVVEVTKPKGSAGSKKTQKDSADAAIITSLKRCLDEYYQSSSSSEI 191
+N KPK S + K ++S + ++ +D SS+++E+
Sbjct: 1185 VRKQQRIKN--NDVKPKISRNAPKAPRNSKKTSNSETITESMDITTSSSTTTEV 1236
Score = 58.2 bits (139), Expect(4) = 3e-76
Identities = 27/49 (55%), Positives = 35/49 (71%)
Frame = -1
Query: 1926 GNSRDDGNRRISYEEFIFQYTIEFSVADLRRSIPSMVDGLKPSQRKVLY 1780
G D + I Y +F+ + I FS+ADL+RSIPSMVDGLKP QRK+L+
Sbjct: 669 GTHLDQSEKLIKYSDFVNKELILFSMADLQRSIPSMVDGLKPGQRKILF 717
Score = 52.0 bits (123), Expect(4) = 3e-76
Identities = 23/47 (48%), Positives = 34/47 (72%)
Frame = -1
Query: 837 VLKEFYALRLRYYEKRKANILLNLSQHILMLENTARYLEMVLEGDII 697
+L+EF+ LRL ++EKRK N+L NL +L LEN R++ V+ G+II
Sbjct: 1016 ILEEFFPLRLEFFEKRKRNLLENLETELLKLENKVRFILAVVNGEII 1062
>ref|XP_023527825.1| DNA topoisomerase 2 isoform X5 [Cucurbita pepo subsp. pepo]
Length = 1269
Score = 166 bits (419), Expect(4) = 3e-76
Identities = 115/300 (38%), Positives = 159/300 (53%), Gaps = 17/300 (5%)
Frame = -3
Query: 1777 SFKRALSEEISLTALIGYVSEHADYHHSGETLGSIIIRMAQNYVSSNNINIFEPIGQFGT 1598
SFKR +E ++ GYVSEH+ YHH ++L S II MAQ++V SNNIN+ P GQFGT
Sbjct: 719 SFKRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGT 778
Query: 1597 GNLGGQDHAPQ--LKTKLNPLSRLLFPRIDDEVLRYFNRGGRSKEPTWYIPIRKWCQRDG 1424
N GG+DHA + TKL+ +R LFP+ DD++L Y N G+S EPTWY+PI +G
Sbjct: 779 RNHGGKDHASSRYIYTKLSNATRFLFPKDDDKLLNYLNEDGQSIEPTWYMPIIPTVLVNG 838
Query: 1423 N------WI*FF--YSKL**TFFYSKL---**TLTMDPCY---XXXXXXXXXXXXXGSYG 1286
+ W + Y+ ++ L MDP Y SY
Sbjct: 839 SEGIGTGWSSYIPNYNPRDIIRNVRRMLNGEAMLPMDPWYRGFSGTIEKTATKEGGTSYT 898
Query: 1285 VRGKVEEINNETLRVTELPVGKWTEPYKKFLNSITAGSHADGTDHMLMARILSL-RTSHN 1109
V G +EE+++ TLR+TELPV +WT YK FL+S+T S + D A+ + + H+
Sbjct: 899 VSGIIEEVSDTTLRITELPVRRWTNDYKDFLDSVTRVSTKNDKDKEKAAKDPFIEQADHH 958
Query: 1108 IKMIKRQI*KSR*SNKKALIMGENLDRAYQKLNLITRISTSNMNLFDADGRMKTFVSLEQ 929
I L M E L + K L T +STSNM+LFDA+G +K + + EQ
Sbjct: 959 SDDKTVSIDVHLTEENMILAMQEGLKK---KFKLTTTVSTSNMHLFDANGVIKKYDTPEQ 1015
Score = 82.8 bits (203), Expect(4) = 3e-76
Identities = 50/174 (28%), Positives = 89/174 (51%), Gaps = 10/174 (5%)
Frame = -3
Query: 682 NRSKFELISELKHQGFDPKPRKGKSAEPRVAGANDDNSEVQE----------DDYEYLTS 533
NR + +L EL+ +GF P P+K KS EP VAGA+++ E +E DYEYL +
Sbjct: 1065 NRRRVDLFHELQEKGFTPFPKKTKSVEPEVAGASNETDEAEESPEVVKGERVSDYEYLLA 1124
Query: 532 IPLSFFSLNHLDKLLAEKMKKMCELNVLKEISCSEMWMMDLVELEKKIDEINRXXXXXXX 353
+ + ++ + +L E+ K E+ L++ + +W+ DL LEK++DE++R
Sbjct: 1125 LAIGTLTIEKVQELCNERDKFKGEVEELRKATPKSLWLKDLDYLEKELDEMDRNDAQADE 1184
Query: 352 XXXXXXXENVVEVTKPKGSAGSKKTQKDSADAAIITSLKRCLDEYYQSSSSSEI 191
+N KPK S + K ++S + ++ +D SS+++E+
Sbjct: 1185 VRKQQRIKN--NDVKPKISRNAPKAPRNSKKTSNSETITESMDITTSSSTTTEV 1236
Score = 58.2 bits (139), Expect(4) = 3e-76
Identities = 27/49 (55%), Positives = 35/49 (71%)
Frame = -1
Query: 1926 GNSRDDGNRRISYEEFIFQYTIEFSVADLRRSIPSMVDGLKPSQRKVLY 1780
G D + I Y +F+ + I FS+ADL+RSIPSMVDGLKP QRK+L+
Sbjct: 669 GTHLDQSEKLIKYSDFVNKELILFSMADLQRSIPSMVDGLKPGQRKILF 717
Score = 52.0 bits (123), Expect(4) = 3e-76
Identities = 23/47 (48%), Positives = 34/47 (72%)
Frame = -1
Query: 837 VLKEFYALRLRYYEKRKANILLNLSQHILMLENTARYLEMVLEGDII 697
+L+EF+ LRL ++EKRK N+L NL +L LEN R++ V+ G+II
Sbjct: 1016 ILEEFFPLRLEFFEKRKRNLLENLETELLKLENKVRFILAVVNGEII 1062
>ref|XP_023527826.1| DNA topoisomerase 2 isoform X6 [Cucurbita pepo subsp. pepo]
Length = 1268
Score = 166 bits (419), Expect(4) = 3e-76
Identities = 115/300 (38%), Positives = 159/300 (53%), Gaps = 17/300 (5%)
Frame = -3
Query: 1777 SFKRALSEEISLTALIGYVSEHADYHHSGETLGSIIIRMAQNYVSSNNINIFEPIGQFGT 1598
SFKR +E ++ GYVSEH+ YHH ++L S II MAQ++V SNNIN+ P GQFGT
Sbjct: 719 SFKRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGT 778
Query: 1597 GNLGGQDHAPQ--LKTKLNPLSRLLFPRIDDEVLRYFNRGGRSKEPTWYIPIRKWCQRDG 1424
N GG+DHA + TKL+ +R LFP+ DD++L Y N G+S EPTWY+PI +G
Sbjct: 779 RNHGGKDHASSRYIYTKLSNATRFLFPKDDDKLLNYLNEDGQSIEPTWYMPIIPTVLVNG 838
Query: 1423 N------WI*FF--YSKL**TFFYSKL---**TLTMDPCY---XXXXXXXXXXXXXGSYG 1286
+ W + Y+ ++ L MDP Y SY
Sbjct: 839 SEGIGTGWSSYIPNYNPRDIIRNVRRMLNGEAMLPMDPWYRGFSGTIEKTATKEGGTSYT 898
Query: 1285 VRGKVEEINNETLRVTELPVGKWTEPYKKFLNSITAGSHADGTDHMLMARILSL-RTSHN 1109
V G +EE+++ TLR+TELPV +WT YK FL+S+T S + D A+ + + H+
Sbjct: 899 VSGIIEEVSDTTLRITELPVRRWTNDYKDFLDSVTRVSTKNDKDKEKAAKDPFIEQADHH 958
Query: 1108 IKMIKRQI*KSR*SNKKALIMGENLDRAYQKLNLITRISTSNMNLFDADGRMKTFVSLEQ 929
I L M E L + K L T +STSNM+LFDA+G +K + + EQ
Sbjct: 959 SDDKTVSIDVHLTEENMILAMQEGLKK---KFKLTTTVSTSNMHLFDANGVIKKYDTPEQ 1015
Score = 82.8 bits (203), Expect(4) = 3e-76
Identities = 50/174 (28%), Positives = 89/174 (51%), Gaps = 10/174 (5%)
Frame = -3
Query: 682 NRSKFELISELKHQGFDPKPRKGKSAEPRVAGANDDNSEVQE----------DDYEYLTS 533
NR + +L EL+ +GF P P+K KS EP VAGA+++ E +E DYEYL +
Sbjct: 1065 NRRRVDLFHELQEKGFTPFPKKTKSVEPEVAGASNETDEAEESPEVVKGERVSDYEYLLA 1124
Query: 532 IPLSFFSLNHLDKLLAEKMKKMCELNVLKEISCSEMWMMDLVELEKKIDEINRXXXXXXX 353
+ + ++ + +L E+ K E+ L++ + +W+ DL LEK++DE++R
Sbjct: 1125 LAIGTLTIEKVQELCNERDKFKGEVEELRKATPKSLWLKDLDYLEKELDEMDRNDAQADE 1184
Query: 352 XXXXXXXENVVEVTKPKGSAGSKKTQKDSADAAIITSLKRCLDEYYQSSSSSEI 191
+N KPK S + K ++S + ++ +D SS+++E+
Sbjct: 1185 VRKQQRIKN--NDVKPKISRNAPKAPRNSKKTSNSETITESMDITTSSSTTTEV 1236
Score = 58.2 bits (139), Expect(4) = 3e-76
Identities = 27/49 (55%), Positives = 35/49 (71%)
Frame = -1
Query: 1926 GNSRDDGNRRISYEEFIFQYTIEFSVADLRRSIPSMVDGLKPSQRKVLY 1780
G D + I Y +F+ + I FS+ADL+RSIPSMVDGLKP QRK+L+
Sbjct: 669 GTHLDQSEKLIKYSDFVNKELILFSMADLQRSIPSMVDGLKPGQRKILF 717
Score = 52.0 bits (123), Expect(4) = 3e-76
Identities = 23/47 (48%), Positives = 34/47 (72%)
Frame = -1
Query: 837 VLKEFYALRLRYYEKRKANILLNLSQHILMLENTARYLEMVLEGDII 697
+L+EF+ LRL ++EKRK N+L NL +L LEN R++ V+ G+II
Sbjct: 1016 ILEEFFPLRLEFFEKRKRNLLENLETELLKLENKVRFILAVVNGEII 1062
>ref|XP_022935015.1| DNA topoisomerase 2 isoform X1 [Cucurbita moschata]
Length = 1492
Score = 166 bits (419), Expect(4) = 4e-76
Identities = 115/300 (38%), Positives = 159/300 (53%), Gaps = 17/300 (5%)
Frame = -3
Query: 1777 SFKRALSEEISLTALIGYVSEHADYHHSGETLGSIIIRMAQNYVSSNNINIFEPIGQFGT 1598
SFKR +E ++ GYVSEH+ YHH ++L S II MAQ++V SNNIN+ P GQFGT
Sbjct: 719 SFKRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGT 778
Query: 1597 GNLGGQDHAPQ--LKTKLNPLSRLLFPRIDDEVLRYFNRGGRSKEPTWYIPIRKWCQRDG 1424
N GG+DHA + TKL+ +R LFP+ DD++L Y N G+S EPTWY+PI +G
Sbjct: 779 RNHGGKDHASSRYIYTKLSNATRFLFPKDDDKLLNYLNEDGQSIEPTWYMPIIPTVLVNG 838
Query: 1423 N------WI*FF--YSKL**TFFYSKL---**TLTMDPCY---XXXXXXXXXXXXXGSYG 1286
+ W + Y+ ++ L MDP Y SY
Sbjct: 839 SEGIGTGWSSYIPNYNPRDIIRNVRRMLNGEAMLPMDPWYRGFSGTIEKTATKEGGTSYT 898
Query: 1285 VRGKVEEINNETLRVTELPVGKWTEPYKKFLNSITAGSHADGTDHMLMARILSL-RTSHN 1109
V G +EE+++ TLR+TELPV +WT YK FL+S+T S + D A+ + + H+
Sbjct: 899 VSGIIEEVSDTTLRITELPVRRWTNDYKDFLDSVTRVSTKNDKDKEKAAKDPFIEQADHH 958
Query: 1108 IKMIKRQI*KSR*SNKKALIMGENLDRAYQKLNLITRISTSNMNLFDADGRMKTFVSLEQ 929
I L M E L + K L T +STSNM+LFDA+G +K + + EQ
Sbjct: 959 SDDKTVSIDVHLTEENMILAMQEGLKK---KFKLTTTVSTSNMHLFDANGVIKKYDTPEQ 1015
Score = 82.4 bits (202), Expect(4) = 4e-76
Identities = 50/174 (28%), Positives = 89/174 (51%), Gaps = 10/174 (5%)
Frame = -3
Query: 682 NRSKFELISELKHQGFDPKPRKGKSAEPRVAGANDDNSEVQE----------DDYEYLTS 533
NR + +L EL+ +GF P P+K KS EP VAGA+++ E +E DYEYL +
Sbjct: 1065 NRRRVDLFHELQEKGFTPFPKKTKSVEPEVAGASNETDEAEESPEVVKGERVSDYEYLLA 1124
Query: 532 IPLSFFSLNHLDKLLAEKMKKMCELNVLKEISCSEMWMMDLVELEKKIDEINRXXXXXXX 353
+ + ++ + +L E+ K E+ L++ + +W+ DL LEK++DE++R
Sbjct: 1125 LAIGTLTIEKVQELCNERDKFKGEVEELRKATPKSLWLKDLDYLEKELDEMDRNDAQADE 1184
Query: 352 XXXXXXXENVVEVTKPKGSAGSKKTQKDSADAAIITSLKRCLDEYYQSSSSSEI 191
+N KPK S + K ++S + ++ +D SS+++E+
Sbjct: 1185 VRKQQRIKN--NDIKPKISRNAPKAPRNSKKTSNSETITESMDITTSSSTTTEV 1236
Score = 58.2 bits (139), Expect(4) = 4e-76
Identities = 27/49 (55%), Positives = 35/49 (71%)
Frame = -1
Query: 1926 GNSRDDGNRRISYEEFIFQYTIEFSVADLRRSIPSMVDGLKPSQRKVLY 1780
G D + I Y +F+ + I FS+ADL+RSIPSMVDGLKP QRK+L+
Sbjct: 669 GTHLDQSEKLIKYSDFVNKELILFSMADLQRSIPSMVDGLKPGQRKILF 717
Score = 52.0 bits (123), Expect(4) = 4e-76
Identities = 23/47 (48%), Positives = 34/47 (72%)
Frame = -1
Query: 837 VLKEFYALRLRYYEKRKANILLNLSQHILMLENTARYLEMVLEGDII 697
+L+EF+ LRL ++EKRK N+L NL +L LEN R++ V+ G+II
Sbjct: 1016 ILEEFFPLRLEFFEKRKRNLLENLETELLKLENKVRFILAVVNGEII 1062
>ref|XP_022982562.1| DNA topoisomerase 2 isoform X1 [Cucurbita maxima]
Length = 1491
Score = 166 bits (419), Expect(4) = 4e-76
Identities = 115/300 (38%), Positives = 159/300 (53%), Gaps = 17/300 (5%)
Frame = -3
Query: 1777 SFKRALSEEISLTALIGYVSEHADYHHSGETLGSIIIRMAQNYVSSNNINIFEPIGQFGT 1598
SFKR +E ++ GYVSEH+ YHH ++L S II MAQ++V SNNIN+ P GQFGT
Sbjct: 718 SFKRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGT 777
Query: 1597 GNLGGQDHAPQ--LKTKLNPLSRLLFPRIDDEVLRYFNRGGRSKEPTWYIPIRKWCQRDG 1424
N GG+DHA + TKL+ +R LFP+ DD++L Y N G+S EPTWY+PI +G
Sbjct: 778 RNHGGKDHASSRYIYTKLSNATRFLFPKDDDKLLNYLNEDGQSIEPTWYMPIIPTVLVNG 837
Query: 1423 N------WI*FF--YSKL**TFFYSKL---**TLTMDPCY---XXXXXXXXXXXXXGSYG 1286
+ W + Y+ ++ L MDP Y SY
Sbjct: 838 SEGIGTGWSSYIPNYNPRDIIRNVRRMLDGEAMLPMDPWYRGFSGTIEKTATKEGGTSYT 897
Query: 1285 VRGKVEEINNETLRVTELPVGKWTEPYKKFLNSITAGSHADGTDHMLMARILSL-RTSHN 1109
V G +EE+++ TLR+TELPV +WT YK FL+S+T S + D A+ + + H+
Sbjct: 898 VSGIIEEVSDTTLRITELPVRRWTNDYKDFLDSVTRVSTKNDKDKEKAAKDPFIEQADHH 957
Query: 1108 IKMIKRQI*KSR*SNKKALIMGENLDRAYQKLNLITRISTSNMNLFDADGRMKTFVSLEQ 929
I L M E L + K L T +STSNM+LFDA+G +K + + EQ
Sbjct: 958 SDDKTVSINVHLTEENMILAMQEGLKK---KFKLTTTVSTSNMHLFDANGVIKKYDTPEQ 1014
Score = 82.4 bits (202), Expect(4) = 4e-76
Identities = 50/174 (28%), Positives = 89/174 (51%), Gaps = 10/174 (5%)
Frame = -3
Query: 682 NRSKFELISELKHQGFDPKPRKGKSAEPRVAGANDDNSEVQE----------DDYEYLTS 533
NR + +L EL+ +GF P P+K KS EP VAGA+++ E +E DYEYL +
Sbjct: 1064 NRRRVDLFHELQEKGFTPFPKKTKSVEPEVAGASNETDEAEESPEVVKGERVSDYEYLLA 1123
Query: 532 IPLSFFSLNHLDKLLAEKMKKMCELNVLKEISCSEMWMMDLVELEKKIDEINRXXXXXXX 353
+ + ++ + +L E+ K E+ L++ + +W+ DL LEK++DE++R
Sbjct: 1124 LAIGTLTIEKVQELCNERDKFKGEVEELRKATPKSLWLKDLDYLEKELDEMDRNDAQADE 1183
Query: 352 XXXXXXXENVVEVTKPKGSAGSKKTQKDSADAAIITSLKRCLDEYYQSSSSSEI 191
+N KPK S + K ++S + ++ +D SS+++E+
Sbjct: 1184 VRKQQRIKN--NDIKPKISRNAPKAPRNSKKTSNSETITESMDIATSSSTTTEV 1235
Score = 58.2 bits (139), Expect(4) = 4e-76
Identities = 27/49 (55%), Positives = 35/49 (71%)
Frame = -1
Query: 1926 GNSRDDGNRRISYEEFIFQYTIEFSVADLRRSIPSMVDGLKPSQRKVLY 1780
G D + I Y +F+ + I FS+ADL+RSIPSMVDGLKP QRK+L+
Sbjct: 668 GTHLDQSEKLIKYSDFVNKELILFSMADLQRSIPSMVDGLKPGQRKILF 716
Score = 52.0 bits (123), Expect(4) = 4e-76
Identities = 23/47 (48%), Positives = 34/47 (72%)
Frame = -1
Query: 837 VLKEFYALRLRYYEKRKANILLNLSQHILMLENTARYLEMVLEGDII 697
+L+EF+ LRL ++EKRK N+L NL +L LEN R++ V+ G+II
Sbjct: 1015 ILEEFFPLRLEFFEKRKRNLLENLETELLKLENKVRFILAVVNGEII 1061
>ref|XP_022935016.1| DNA topoisomerase 2 isoform X2 [Cucurbita moschata]
Length = 1491
Score = 166 bits (419), Expect(4) = 4e-76
Identities = 115/300 (38%), Positives = 159/300 (53%), Gaps = 17/300 (5%)
Frame = -3
Query: 1777 SFKRALSEEISLTALIGYVSEHADYHHSGETLGSIIIRMAQNYVSSNNINIFEPIGQFGT 1598
SFKR +E ++ GYVSEH+ YHH ++L S II MAQ++V SNNIN+ P GQFGT
Sbjct: 719 SFKRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGT 778
Query: 1597 GNLGGQDHAPQ--LKTKLNPLSRLLFPRIDDEVLRYFNRGGRSKEPTWYIPIRKWCQRDG 1424
N GG+DHA + TKL+ +R LFP+ DD++L Y N G+S EPTWY+PI +G
Sbjct: 779 RNHGGKDHASSRYIYTKLSNATRFLFPKDDDKLLNYLNEDGQSIEPTWYMPIIPTVLVNG 838
Query: 1423 N------WI*FF--YSKL**TFFYSKL---**TLTMDPCY---XXXXXXXXXXXXXGSYG 1286
+ W + Y+ ++ L MDP Y SY
Sbjct: 839 SEGIGTGWSSYIPNYNPRDIIRNVRRMLNGEAMLPMDPWYRGFSGTIEKTATKEGGTSYT 898
Query: 1285 VRGKVEEINNETLRVTELPVGKWTEPYKKFLNSITAGSHADGTDHMLMARILSL-RTSHN 1109
V G +EE+++ TLR+TELPV +WT YK FL+S+T S + D A+ + + H+
Sbjct: 899 VSGIIEEVSDTTLRITELPVRRWTNDYKDFLDSVTRVSTKNDKDKEKAAKDPFIEQADHH 958
Query: 1108 IKMIKRQI*KSR*SNKKALIMGENLDRAYQKLNLITRISTSNMNLFDADGRMKTFVSLEQ 929
I L M E L + K L T +STSNM+LFDA+G +K + + EQ
Sbjct: 959 SDDKTVSIDVHLTEENMILAMQEGLKK---KFKLTTTVSTSNMHLFDANGVIKKYDTPEQ 1015
Score = 82.4 bits (202), Expect(4) = 4e-76
Identities = 50/174 (28%), Positives = 89/174 (51%), Gaps = 10/174 (5%)
Frame = -3
Query: 682 NRSKFELISELKHQGFDPKPRKGKSAEPRVAGANDDNSEVQE----------DDYEYLTS 533
NR + +L EL+ +GF P P+K KS EP VAGA+++ E +E DYEYL +
Sbjct: 1065 NRRRVDLFHELQEKGFTPFPKKTKSVEPEVAGASNETDEAEESPEVVKGERVSDYEYLLA 1124
Query: 532 IPLSFFSLNHLDKLLAEKMKKMCELNVLKEISCSEMWMMDLVELEKKIDEINRXXXXXXX 353
+ + ++ + +L E+ K E+ L++ + +W+ DL LEK++DE++R
Sbjct: 1125 LAIGTLTIEKVQELCNERDKFKGEVEELRKATPKSLWLKDLDYLEKELDEMDRNDAQADE 1184
Query: 352 XXXXXXXENVVEVTKPKGSAGSKKTQKDSADAAIITSLKRCLDEYYQSSSSSEI 191
+N KPK S + K ++S + ++ +D SS+++E+
Sbjct: 1185 VRKQQRIKN--NDIKPKISRNAPKAPRNSKKTSNSETITESMDITTSSSTTTEV 1236
Score = 58.2 bits (139), Expect(4) = 4e-76
Identities = 27/49 (55%), Positives = 35/49 (71%)
Frame = -1
Query: 1926 GNSRDDGNRRISYEEFIFQYTIEFSVADLRRSIPSMVDGLKPSQRKVLY 1780
G D + I Y +F+ + I FS+ADL+RSIPSMVDGLKP QRK+L+
Sbjct: 669 GTHLDQSEKLIKYSDFVNKELILFSMADLQRSIPSMVDGLKPGQRKILF 717
Score = 52.0 bits (123), Expect(4) = 4e-76
Identities = 23/47 (48%), Positives = 34/47 (72%)
Frame = -1
Query: 837 VLKEFYALRLRYYEKRKANILLNLSQHILMLENTARYLEMVLEGDII 697
+L+EF+ LRL ++EKRK N+L NL +L LEN R++ V+ G+II
Sbjct: 1016 ILEEFFPLRLEFFEKRKRNLLENLETELLKLENKVRFILAVVNGEII 1062
>ref|XP_022982563.1| DNA topoisomerase 2 isoform X2 [Cucurbita maxima]
Length = 1490
Score = 166 bits (419), Expect(4) = 4e-76
Identities = 115/300 (38%), Positives = 159/300 (53%), Gaps = 17/300 (5%)
Frame = -3
Query: 1777 SFKRALSEEISLTALIGYVSEHADYHHSGETLGSIIIRMAQNYVSSNNINIFEPIGQFGT 1598
SFKR +E ++ GYVSEH+ YHH ++L S II MAQ++V SNNIN+ P GQFGT
Sbjct: 718 SFKRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGT 777
Query: 1597 GNLGGQDHAPQ--LKTKLNPLSRLLFPRIDDEVLRYFNRGGRSKEPTWYIPIRKWCQRDG 1424
N GG+DHA + TKL+ +R LFP+ DD++L Y N G+S EPTWY+PI +G
Sbjct: 778 RNHGGKDHASSRYIYTKLSNATRFLFPKDDDKLLNYLNEDGQSIEPTWYMPIIPTVLVNG 837
Query: 1423 N------WI*FF--YSKL**TFFYSKL---**TLTMDPCY---XXXXXXXXXXXXXGSYG 1286
+ W + Y+ ++ L MDP Y SY
Sbjct: 838 SEGIGTGWSSYIPNYNPRDIIRNVRRMLDGEAMLPMDPWYRGFSGTIEKTATKEGGTSYT 897
Query: 1285 VRGKVEEINNETLRVTELPVGKWTEPYKKFLNSITAGSHADGTDHMLMARILSL-RTSHN 1109
V G +EE+++ TLR+TELPV +WT YK FL+S+T S + D A+ + + H+
Sbjct: 898 VSGIIEEVSDTTLRITELPVRRWTNDYKDFLDSVTRVSTKNDKDKEKAAKDPFIEQADHH 957
Query: 1108 IKMIKRQI*KSR*SNKKALIMGENLDRAYQKLNLITRISTSNMNLFDADGRMKTFVSLEQ 929
I L M E L + K L T +STSNM+LFDA+G +K + + EQ
Sbjct: 958 SDDKTVSINVHLTEENMILAMQEGLKK---KFKLTTTVSTSNMHLFDANGVIKKYDTPEQ 1014
Score = 82.4 bits (202), Expect(4) = 4e-76
Identities = 50/174 (28%), Positives = 89/174 (51%), Gaps = 10/174 (5%)
Frame = -3
Query: 682 NRSKFELISELKHQGFDPKPRKGKSAEPRVAGANDDNSEVQE----------DDYEYLTS 533
NR + +L EL+ +GF P P+K KS EP VAGA+++ E +E DYEYL +
Sbjct: 1064 NRRRVDLFHELQEKGFTPFPKKTKSVEPEVAGASNETDEAEESPEVVKGERVSDYEYLLA 1123
Query: 532 IPLSFFSLNHLDKLLAEKMKKMCELNVLKEISCSEMWMMDLVELEKKIDEINRXXXXXXX 353
+ + ++ + +L E+ K E+ L++ + +W+ DL LEK++DE++R
Sbjct: 1124 LAIGTLTIEKVQELCNERDKFKGEVEELRKATPKSLWLKDLDYLEKELDEMDRNDAQADE 1183
Query: 352 XXXXXXXENVVEVTKPKGSAGSKKTQKDSADAAIITSLKRCLDEYYQSSSSSEI 191
+N KPK S + K ++S + ++ +D SS+++E+
Sbjct: 1184 VRKQQRIKN--NDIKPKISRNAPKAPRNSKKTSNSETITESMDIATSSSTTTEV 1235
Score = 58.2 bits (139), Expect(4) = 4e-76
Identities = 27/49 (55%), Positives = 35/49 (71%)
Frame = -1
Query: 1926 GNSRDDGNRRISYEEFIFQYTIEFSVADLRRSIPSMVDGLKPSQRKVLY 1780
G D + I Y +F+ + I FS+ADL+RSIPSMVDGLKP QRK+L+
Sbjct: 668 GTHLDQSEKLIKYSDFVNKELILFSMADLQRSIPSMVDGLKPGQRKILF 716
Score = 52.0 bits (123), Expect(4) = 4e-76
Identities = 23/47 (48%), Positives = 34/47 (72%)
Frame = -1
Query: 837 VLKEFYALRLRYYEKRKANILLNLSQHILMLENTARYLEMVLEGDII 697
+L+EF+ LRL ++EKRK N+L NL +L LEN R++ V+ G+II
Sbjct: 1015 ILEEFFPLRLEFFEKRKRNLLENLETELLKLENKVRFILAVVNGEII 1061
>ref|XP_020212372.1| DNA topoisomerase 2 [Cajanus cajan]
Length = 1480
Score = 251 bits (642), Expect(2) = 3e-75
Identities = 213/609 (34%), Positives = 293/609 (48%), Gaps = 72/609 (11%)
Frame = -3
Query: 1777 SFKRALSEEISLTALIGYVSEHADYHHSGETLGSIIIRMAQNYVSSNNINIFEPIGQFGT 1598
SFK+ L +EI + IGYVSEH+ YHH ++L S II MAQ++V SNNIN+ +P GQFGT
Sbjct: 712 SFKKKLFKEIKVGQFIGYVSEHSAYHHGEQSLASTIINMAQDFVGSNNINLLKPNGQFGT 771
Query: 1597 GNLGGQDHAP--QLKTKLNPLSRLLFPRIDDEVLRYFNRGGRSKEPTWYIPIRKWCQRDG 1424
NLGG+DHA + T+LN +R +F DD++L Y N G+S EP WYIPI +G
Sbjct: 772 RNLGGKDHASARYIYTELNTFTRCIFHEDDDKLLEYLNEDGKSIEPNWYIPIIPLVLVNG 831
Query: 1423 N------WI*FF--YSKL**TFFYSKL**TLT---MDPCYXXXXXXXXXXXXXGSYGVRG 1277
+ W + Y+ +L T M P Y G Y V G
Sbjct: 832 SEGIGTGWSSYIPNYNPRDIIANVRRLLSGETMVPMHPWYKGFKGIIERSPKEGGYVVNG 891
Query: 1276 KVEEINNETLRVTELPVGKWTEPYKKFLNSITAGSHADGTDHMLMARILSLRTSHNIKMI 1097
VEE++ +T R+TELP+ KWT+ YK+FL SIT DG+ ++ I R + + ++
Sbjct: 892 LVEEMDEQTFRITELPIRKWTQDYKQFLESIT-----DGSPNVKDPLIEDFRQNGDDAIV 946
Query: 1096 KRQI*KSR*SNKKALIMGENLDRAYQKLNLITRISTSNMNLFDADGRMKTFVSLEQSKAL 917
++ ++K A IM E L +K L + +STSNM+LFDA+G++K + + EQ
Sbjct: 947 DIEVRMK--ADKIAGIMQEGL---LKKFKLTSTLSTSNMHLFDAEGKIKKYENPEQILEE 1001
Query: 916 FCRVVLKNNNINSVFYVLIILYHFLCSSERVLCTTAALL*KTKGKYTAQPFPTYSDVGEH 737
F + L+ Y H L + ER+L L+ K ++
Sbjct: 1002 FFPLRLE--------YYERRKKHILENLERLL-----LVLDNKVRFI------------- 1035
Query: 736 S*VSRNGFGRRYYYLTWLNRSKFELISELKHQGFDPKPRKGKSAEPRVAGANDD-----N 572
G + NR K EL+ EL+ +GF P PRKGKSAEP+VAGA+D+ N
Sbjct: 1036 -------LGVVNGEIIVSNRKKAELLIELQQKGFTPMPRKGKSAEPQVAGADDEQDDDNN 1088
Query: 571 SEVQE---------DDYEYLTSIPLSFFSLNHLDKLLAEKMKKMCELNVLKEISCSEMWM 419
+ QE DYEYL S+ + +L + KLLAEK +K E +LK MW+
Sbjct: 1089 TREQETSSVEGAKRGDYEYLLSMSIGTLTLESVQKLLAEKDEKEKEFEILKATPSKSMWL 1148
Query: 418 MDLVELEKKIDE---------------INR-----------XXXXXXXXXXXXXXENVVE 317
DL ELEKK+DE NR +NVVE
Sbjct: 1149 KDLDELEKKLDESASKEAEEERKRSSQANRKTSYRGETKAAAKKPPQPRKYTKKAKNVVE 1208
Query: 316 ---------------VTKPKGSAGSK----KTQKDSADAAIITSLKRCLDEYYQSSSSSE 194
V KPKG AGSK TQ AD I++ +R Y +SS+
Sbjct: 1209 PENDNSPMEVENVAEVPKPKGRAGSKNSLSSTQTTVADDEILSLQERL--AAYNFGASSD 1266
Query: 193 ITGGDMETE 167
I+ G METE
Sbjct: 1267 ISAG-METE 1274
Score = 62.0 bits (149), Expect(2) = 3e-75
Identities = 30/49 (61%), Positives = 38/49 (77%)
Frame = -1
Query: 1926 GNSRDDGNRRISYEEFIFQYTIEFSVADLRRSIPSMVDGLKPSQRKVLY 1780
GN RD +R I+Y +F+ + I FS ADL+RSIPSMVDGLKP QRK+L+
Sbjct: 662 GNHRDHMDRYINYRDFVNKELILFSRADLQRSIPSMVDGLKPGQRKILF 710
>ref|XP_004499912.1| PREDICTED: DNA topoisomerase 2 [Cicer arietinum]
ref|XP_004517303.1| PREDICTED: DNA topoisomerase 2 [Cicer arietinum]
Length = 1472
Score = 253 bits (646), Expect(2) = 1e-74
Identities = 208/609 (34%), Positives = 288/609 (47%), Gaps = 69/609 (11%)
Frame = -3
Query: 1777 SFKRALSEEISLTALIGYVSEHADYHHSGETLGSIIIRMAQNYVSSNNINIFEPIGQFGT 1598
SFK+ L +EI + IGYVSEH+ YHH ++L S II MAQ++V SNNIN+ +P GQFGT
Sbjct: 709 SFKKKLFKEIKVGQFIGYVSEHSCYHHGEQSLASTIIGMAQDFVGSNNINLLKPNGQFGT 768
Query: 1597 GNLGGQDHAPQ--LKTKLNPLSRLLFPRIDDEVLRYFNRGGRSKEPTWYIPIRKWCQRDG 1424
NLGG+DHA + T+L+ ++R LF DD++L Y N G+S EP WYIPI +G
Sbjct: 769 RNLGGKDHASSRYIYTELSAVTRCLFHEDDDKLLDYLNEDGKSIEPNWYIPIIPLVLVNG 828
Query: 1423 N------WI*FF--YSKL**TFFYSKL---**TLTMDPCYXXXXXXXXXXXXXGSYGVRG 1277
+ W + Y+ +L + MDP Y G Y V G
Sbjct: 829 SEGIGTGWSSYIPNYNPREIIANVRRLLNGEAMVPMDPWYRGFRGTIEKSAKEGGYIVNG 888
Query: 1276 KVEEINNETLRVTELPVGKWTEPYKKFLNSITAGSHADGTDHMLMARILSLRTSHNIKMI 1097
V EI+ +T R+TELP+ KWT+ YK+FL S+T DG ++ I R + + ++
Sbjct: 889 SVMEIDEQTFRITELPIRKWTQDYKQFLESVT-----DGAPNVKDPLIEDFRQNGDDAIV 943
Query: 1096 KRQI*KSR*SNKKALIMGENLDRAYQKLNLITRISTSNMNLFDADGRMKTFVSLEQSKAL 917
++ +K A IM E L ++K L + ISTSNM+LFDA+G++K F + EQ
Sbjct: 944 DIEV--KMKLDKIATIMQEGL---FKKFKLSSTISTSNMHLFDAEGKIKKFDTPEQILEE 998
Query: 916 FCRVVLKNNNINSVFYVLIILYHFLCSSERVLCTTAALL*KTKGKYTAQPFPTYSDVGEH 737
F + L+ Y + L + ER+L L+ K ++
Sbjct: 999 FFPLRLE--------YYEKRKKYILANLERLL-----LILDNKVRFI------------- 1032
Query: 736 S*VSRNGFGRRYYYLTWLNRSKFELISELKHQGFDPKPRKGKSAEPRVAGANDDNSEVQE 557
G + NR K +L+ ELK +GF P PRKGKSAEP+VAGA DDNSE QE
Sbjct: 1033 -------LGVVSGEIIVSNRKKADLLIELKKKGFTPMPRKGKSAEPQVAGAIDDNSEEQE 1085
Query: 556 D-----------------DYEYLTSIPLSFFSLNHLDKLLAEKMKKMCELNVLKEISCSE 428
D DYEYL S+P+ +L + KL EK +K E +L +
Sbjct: 1086 DEQDTVSRPVSVEGATWGDYEYLLSLPIGTLTLESVQKLRDEKAEKEKEFEILMGTPTTS 1145
Query: 427 MWMMDLVELEKKIDEIN---------------------------------RXXXXXXXXX 347
+WM DL E E K+DE++ R
Sbjct: 1146 LWMKDLDEFETKLDELDNKDAEDERKRSSQASKKAPNGFASKAAKKPPQPRKNTKKAKTV 1205
Query: 346 XXXXXENVVEV------TKPKGSAGSKKTQKDSADAAIITSLKRCLDEYYQSSSSSEITG 185
+ +E+ KPKG A SK TQK++ D I SL+ L Y SS +
Sbjct: 1206 EPENDNSSMEIENPVEVAKPKGRAVSKNTQKETDDD--ILSLQERLAAYNFESSGEK--S 1261
Query: 184 GDMETEVAA 158
METE A
Sbjct: 1262 AAMETEEPA 1270
Score = 58.2 bits (139), Expect(2) = 1e-74
Identities = 28/49 (57%), Positives = 37/49 (75%)
Frame = -1
Query: 1926 GNSRDDGNRRISYEEFIFQYTIEFSVADLRRSIPSMVDGLKPSQRKVLY 1780
G RD + I+Y++F+ + I FS ADL+RSIPSMVDGLKP QRK+L+
Sbjct: 659 GTFRDHDAKLINYKDFVNKELILFSRADLQRSIPSMVDGLKPGQRKILF 707
>ref|XP_022152631.1| DNA topoisomerase 2 isoform X1 [Momordica charantia]
Length = 1492
Score = 164 bits (415), Expect(4) = 9e-73
Identities = 111/300 (37%), Positives = 160/300 (53%), Gaps = 17/300 (5%)
Frame = -3
Query: 1777 SFKRALSEEISLTALIGYVSEHADYHHSGETLGSIIIRMAQNYVSSNNINIFEPIGQFGT 1598
SFKR +E ++ GYVSEH+ YHH ++L S II MAQ++V SNNIN+ P GQFGT
Sbjct: 719 SFKRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGT 778
Query: 1597 GNLGGQDHAPQ--LKTKLNPLSRLLFPRIDDEVLRYFNRGGRSKEPTWYIPIRKWCQRDG 1424
N GG+DHA + TKL+ ++R LFPR DD +L Y N G+S EPTWY+PI +G
Sbjct: 779 RNHGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNG 838
Query: 1423 N------WI*FF--YSKL**TFFYSKL---**TLTMDPCY---XXXXXXXXXXXXXGSYG 1286
+ W + Y+ +L + MDP Y SY
Sbjct: 839 SEGIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGIIEKTATKEGGVSYT 898
Query: 1285 VRGKVEEINNETLRVTELPVGKWTEPYKKFLNSITAGSHADGTDHMLMARILSLRTSH-N 1109
V G +EE+++ TLR+TELPV +WT YK+FL+S+T + + + + H +
Sbjct: 899 VSGIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHSD 958
Query: 1108 IKMIKRQI*KSR*SNKKALIMGENLDRAYQKLNLITRISTSNMNLFDADGRMKTFVSLEQ 929
K + + + L + E L + K L T +STSNM+LFD++G +K + + EQ
Sbjct: 959 DKTVSIDVHLT--EENMILALQEGLKK---KFKLTTTVSTSNMHLFDSNGVIKKYDTPEQ 1013
Score = 71.2 bits (173), Expect(4) = 9e-73
Identities = 36/112 (32%), Positives = 65/112 (58%), Gaps = 9/112 (8%)
Frame = -3
Query: 682 NRSKFELISELKHQGFDPKPRKGKSAEPRVAGANDDNSEVQE---------DDYEYLTSI 530
NR + +L EL+ +GF P P+K KS EP VAGA+++ E +E DY YL ++
Sbjct: 1063 NRRRVDLFHELQAKGFTPFPKKTKSVEPEVAGASNETDEAEESPEAKGERVSDYGYLLAL 1122
Query: 529 PLSFFSLNHLDKLLAEKMKKMCELNVLKEISCSEMWMMDLVELEKKIDEINR 374
+ ++ + +L ++ + E+ L++ + +W+ DL LEK++DE++R
Sbjct: 1123 AIGTLTIEKVQELCNDRDELKGEVEDLRKATPKSLWLKDLDTLEKELDEMDR 1174
Score = 55.8 bits (133), Expect(4) = 9e-73
Identities = 26/49 (53%), Positives = 35/49 (71%)
Frame = -1
Query: 1926 GNSRDDGNRRISYEEFIFQYTIEFSVADLRRSIPSMVDGLKPSQRKVLY 1780
G D + I Y +F+ + I FS+ADL+RSIPS+VDGLKP QRK+L+
Sbjct: 669 GTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILF 717
Score = 55.8 bits (133), Expect(4) = 9e-73
Identities = 24/47 (51%), Positives = 35/47 (74%)
Frame = -1
Query: 837 VLKEFYALRLRYYEKRKANILLNLSQHILMLENTARYLEMVLEGDII 697
+L+EF+ LRL YYEKRK N+L NL + +L LEN R++ V+ G+I+
Sbjct: 1014 ILEEFFPLRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIV 1060
>ref|XP_022152632.1| DNA topoisomerase 2 isoform X2 [Momordica charantia]
Length = 1490
Score = 164 bits (415), Expect(4) = 9e-73
Identities = 111/300 (37%), Positives = 160/300 (53%), Gaps = 17/300 (5%)
Frame = -3
Query: 1777 SFKRALSEEISLTALIGYVSEHADYHHSGETLGSIIIRMAQNYVSSNNINIFEPIGQFGT 1598
SFKR +E ++ GYVSEH+ YHH ++L S II MAQ++V SNNIN+ P GQFGT
Sbjct: 719 SFKRNFIKEAKVSQFSGYVSEHSAYHHGEQSLASTIIGMAQDFVGSNNINLLLPNGQFGT 778
Query: 1597 GNLGGQDHAPQ--LKTKLNPLSRLLFPRIDDEVLRYFNRGGRSKEPTWYIPIRKWCQRDG 1424
N GG+DHA + TKL+ ++R LFPR DD +L Y N G+S EPTWY+PI +G
Sbjct: 779 RNHGGKDHASSRYIYTKLSNVTRFLFPRDDDRLLNYLNEDGQSIEPTWYMPIIPTVLVNG 838
Query: 1423 N------WI*FF--YSKL**TFFYSKL---**TLTMDPCY---XXXXXXXXXXXXXGSYG 1286
+ W + Y+ +L + MDP Y SY
Sbjct: 839 SEGIGTGWSSYVPNYNPRDIITNVKRLLDGETMMPMDPWYKGFSGIIEKTATKEGGVSYT 898
Query: 1285 VRGKVEEINNETLRVTELPVGKWTEPYKKFLNSITAGSHADGTDHMLMARILSLRTSH-N 1109
V G +EE+++ TLR+TELPV +WT YK+FL+S+T + + + + H +
Sbjct: 899 VSGIIEEVSDTTLRITELPVRRWTNDYKEFLDSVTRVATKNDKEKAAKDPFIEQADHHSD 958
Query: 1108 IKMIKRQI*KSR*SNKKALIMGENLDRAYQKLNLITRISTSNMNLFDADGRMKTFVSLEQ 929
K + + + L + E L + K L T +STSNM+LFD++G +K + + EQ
Sbjct: 959 DKTVSIDVHLT--EENMILALQEGLKK---KFKLTTTVSTSNMHLFDSNGVIKKYDTPEQ 1013
Score = 71.2 bits (173), Expect(4) = 9e-73
Identities = 36/112 (32%), Positives = 65/112 (58%), Gaps = 9/112 (8%)
Frame = -3
Query: 682 NRSKFELISELKHQGFDPKPRKGKSAEPRVAGANDDNSEVQE---------DDYEYLTSI 530
NR + +L EL+ +GF P P+K KS EP VAGA+++ E +E DY YL ++
Sbjct: 1063 NRRRVDLFHELQAKGFTPFPKKTKSVEPEVAGASNETDEAEESPEAKGERVSDYGYLLAL 1122
Query: 529 PLSFFSLNHLDKLLAEKMKKMCELNVLKEISCSEMWMMDLVELEKKIDEINR 374
+ ++ + +L ++ + E+ L++ + +W+ DL LEK++DE++R
Sbjct: 1123 AIGTLTIEKVQELCNDRDELKGEVEDLRKATPKSLWLKDLDTLEKELDEMDR 1174
Score = 55.8 bits (133), Expect(4) = 9e-73
Identities = 26/49 (53%), Positives = 35/49 (71%)
Frame = -1
Query: 1926 GNSRDDGNRRISYEEFIFQYTIEFSVADLRRSIPSMVDGLKPSQRKVLY 1780
G D + I Y +F+ + I FS+ADL+RSIPS+VDGLKP QRK+L+
Sbjct: 669 GTHLDQTEKLIKYSDFVNKELILFSMADLQRSIPSVVDGLKPGQRKILF 717
Score = 55.8 bits (133), Expect(4) = 9e-73
Identities = 24/47 (51%), Positives = 35/47 (74%)
Frame = -1
Query: 837 VLKEFYALRLRYYEKRKANILLNLSQHILMLENTARYLEMVLEGDII 697
+L+EF+ LRL YYEKRK N+L NL + +L LEN R++ V+ G+I+
Sbjct: 1014 ILEEFFPLRLEYYEKRKMNLLENLERELLQLENKVRFILAVVNGEIV 1060
>ref|XP_019454379.1| PREDICTED: DNA topoisomerase 2 [Lupinus angustifolius]
gb|OIW05552.1| hypothetical protein TanjilG_23338 [Lupinus angustifolius]
Length = 1488
Score = 241 bits (614), Expect(2) = 2e-72
Identities = 177/494 (35%), Positives = 253/494 (51%), Gaps = 29/494 (5%)
Frame = -3
Query: 1777 SFKRALSEEISLTALIGYVSEHADYHHSGETLGSIIIRMAQNYVSSNNINIFEPIGQFGT 1598
SFK+ L +EI + IGYVSE + YHH ++L S II MAQN+V SNNIN+ +P GQFGT
Sbjct: 718 SFKKKLFKEIKVAQFIGYVSEQSAYHHGEQSLASTIIGMAQNFVGSNNINLLQPNGQFGT 777
Query: 1597 GNLGGQDHAP--QLKTKLNPLSRLLFPRIDDEVLRYFNRGGRSKEPTWYIPI-----RKW 1439
GG++HA + T+L P++R LFP DD +L Y N G+S EP WYIP+
Sbjct: 778 REEGGKNHASARYIFTELCPITRCLFPEDDDNLLEYLNEDGKSIEPNWYIPVIPTVLANG 837
Query: 1438 CQRDG-NWI*FF--YSKL**TFFYSKL---**TLTMDPCYXXXXXXXXXXXXXGSYGVRG 1277
C+ G W + Y+ +L + MDP + G Y V G
Sbjct: 838 CEGIGTGWSSYIPNYNPKDIIANVRRLMNGEAMIPMDPWFRGYNGIIEKSEKEGGYIVSG 897
Query: 1276 KVEEINNETLRVTELPVGKWTEPYKKFLNSITAGSHADGTDHMLMARILSLRTSHNIKMI 1097
+E+++ +T R+TELP+ KWT+ YK+FL+SIT +G+ ++ I R + + +M+
Sbjct: 898 TIEDVDEQTFRITELPIRKWTKDYKQFLDSIT-----EGSPNVKDPLIEDYRVNGDEEML 952
Query: 1096 KRQI*KSR*SNKKALIMGENLDRAYQKLNLITRISTSNMNLFDADGRMKTFVSLEQSKAL 917
I +K A+IM E L +K L + ISTSNM+LFDA+G++K + + EQ
Sbjct: 953 --DILVRMKEDKVAMIMKEGL---MKKFKLTSTISTSNMHLFDAEGKIKKYDNPEQ---- 1003
Query: 916 FCRVVLKNNNINSVFYVLIILYHFLCSSERVLCTTAALL*KTKGKYTAQPFPTYSDVGEH 737
I F+ L + Y + + L T LL K
Sbjct: 1004 ----------IIEEFFPLRLEY-YEHRKKYKLDTLQRLLLTLDNK--------------- 1037
Query: 736 S*VSRNGFGRRYYYLTWLNRSKFELISELKHQGFDPKPRKGKSAEPRVAGANDDNSEVQE 557
+R G + NR K +L+ ELK +GF P PR KS+EP+VAGA+D NSE E
Sbjct: 1038 ---ARFILGVVSGEIIVSNRKKADLLLELKQKGFTPMPRNAKSSEPQVAGADDGNSEELE 1094
Query: 556 D----------------DYEYLTSIPLSFFSLNHLDKLLAEKMKKMCELNVLKEISCSEM 425
+ DYEYL S+ + ++ + KLLAEK +K +L +LK S M
Sbjct: 1095 NDNSREQESGSEAAKIGDYEYLLSMSIGTLTMESVQKLLAEKDEKWKQLEILKATSPQSM 1154
Query: 424 WMMDLVELEKKIDE 383
W DL +LEKK+D+
Sbjct: 1155 WTKDLDDLEKKLDQ 1168
Score = 63.2 bits (152), Expect(2) = 2e-72
Identities = 31/48 (64%), Positives = 37/48 (77%)
Frame = -1
Query: 1923 NSRDDGNRRISYEEFIFQYTIEFSVADLRRSIPSMVDGLKPSQRKVLY 1780
N RD +RISY +F+ + I FS ADL+RSIPSMVDGLKP QRK+LY
Sbjct: 669 NFRDHKEKRISYTDFVNKELILFSRADLQRSIPSMVDGLKPGQRKILY 716
>ref|XP_003601827.1| DNA topoisomerase IV subunit A [Medicago truncatula]
gb|AES72078.1| DNA topoisomerase IV subunit A [Medicago truncatula]
Length = 1345
Score = 241 bits (615), Expect(2) = 6e-69
Identities = 173/487 (35%), Positives = 252/487 (51%), Gaps = 20/487 (4%)
Frame = -3
Query: 1777 SFKRALSEEISLTALIGYVSEHADYHHSGETLGSIIIRMAQNYVSSNNINIFEPIGQFGT 1598
SFK+ L +EI ++ IGYVS H+ YHH ++L + II MAQ++V NNIN+ P GQFGT
Sbjct: 657 SFKKKLYKEIKVSQFIGYVSLHSAYHHGEQSLANTIIGMAQDFVGGNNINLLNPNGQFGT 716
Query: 1597 GNLGGQDHA--PQLKTKLNPLSRLLFPRIDDEVLRYFNRGGRSKEPTWYIPIRKWCQRDG 1424
NLGG+DHA + T+LN ++R LF DD++L+Y N G+S EP WYIPI +G
Sbjct: 717 RNLGGKDHASPKYIYTELNIVTRFLFHEDDDKLLKYLNEEGKSIEPNWYIPIIPLVLVNG 776
Query: 1423 N------WI*FF--YSKL**TFFYSKL---**TLTMDPCYXXXXXXXXXXXXXGSYGVRG 1277
+ W + Y+ +L + MDP Y G Y V G
Sbjct: 777 SEEIGTGWSSYIPNYNPREIIANVRRLLNEEEMVPMDPWYRGFRGTIEKSMKQGGYRVNG 836
Query: 1276 KVEEINNETLRVTELPVGKWTEPYKKFLNSITAGSHADGTDHMLMARILSLRTSHNIKMI 1097
+ +I+ +T R+TELP+ KWTE YK+FL SIT +G ++ I R + + +I
Sbjct: 837 SMVKIDEQTFRITELPIRKWTEDYKQFLESIT-----EGAPNVNDPFIEDFRQNGDDAII 891
Query: 1096 KRQI*KSR*SNKKALIMGENLDRAYQKLNLITRISTSNMNLFDADGRMKTFVSLEQSKAL 917
++ K + +++ G ++K L + +STSNM+LFDA+G++K + + EQ
Sbjct: 892 DIEV-KMKPDKVASVMQG-----LFKKFKLTSIVSTSNMHLFDAEGKIKKYDTPEQ---- 941
Query: 916 FCRVVLKNNNINSVFYVLIILY------HFLCSSERVLCTTAALL*KTKGKYTAQPFPTY 755
I FY L + Y + L + ER+L L+ K ++
Sbjct: 942 ----------ILEEFYPLRMDYYQRRKKYMLANLERLL-----LILDNKVRFI------- 979
Query: 754 SDVGEHS*VSRNGFGRRYYYLTWLNRSKFELISELKHQGFDPKPRKGKSAEPRVAGANDD 575
G + +NR K EL+ ELK +GF P RKGKSAEP+V GAND+
Sbjct: 980 -------------LGVVNGEIIMINRKKAELLIELKQKGFSPMLRKGKSAEPQVVGANDE 1026
Query: 574 NSE-VQEDDYEYLTSIPLSFFSLNHLDKLLAEKMKKMCELNVLKEISCSEMWMMDLVELE 398
+SE DYEY S+P+ +L + KLL EK +K E VL +W+ DL E E
Sbjct: 1027 SSEGATWGDYEYFLSLPIGTLTLESVQKLLDEKAEKEKEHEVLMGTPTKSLWLKDLDEFE 1086
Query: 397 KKIDEIN 377
KK+DE++
Sbjct: 1087 KKLDELD 1093
Score = 51.2 bits (121), Expect(2) = 6e-69
Identities = 25/39 (64%), Positives = 31/39 (79%)
Frame = -1
Query: 1896 ISYEEFIFQYTIEFSVADLRRSIPSMVDGLKPSQRKVLY 1780
I Y+ FI + I FS A+L+RSIPSMVDGLKP QRK+L+
Sbjct: 617 IRYKSFIDRELILFSRAELQRSIPSMVDGLKPGQRKILF 655