BLASTX nr result
ID: Angelica27_contig00028853
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Angelica27_contig00028853
(1042 letters)
Database: ./nr
115,041,592 sequences; 42,171,959,267 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
XP_017216574.1 PREDICTED: putative dual specificity protein phos... 481 e-169
XP_011089735.1 PREDICTED: putative dual specificity protein phos... 352 e-118
XP_006464441.1 PREDICTED: putative dual specificity protein phos... 348 e-116
XP_011031866.1 PREDICTED: putative dual specificity protein phos... 345 e-115
XP_015388085.1 PREDICTED: putative dual specificity protein phos... 342 e-114
XP_006375113.1 hypothetical protein POPTR_0014s04500g [Populus t... 341 e-113
XP_019188798.1 PREDICTED: putative dual specificity protein phos... 339 e-113
XP_011017326.1 PREDICTED: putative dual specificity protein phos... 335 e-111
XP_002301226.2 hypothetical protein POPTR_0002s13750g [Populus t... 333 e-110
XP_010275392.1 PREDICTED: putative dual specificity protein phos... 333 e-110
XP_012083664.1 PREDICTED: putative dual specificity protein phos... 332 e-109
CDP09450.1 unnamed protein product [Coffea canephora] 330 e-109
XP_017615966.1 PREDICTED: putative dual specificity protein phos... 330 e-109
XP_012489699.1 PREDICTED: putative dual specificity protein phos... 329 e-109
KZV45079.1 hypothetical protein F511_29400 [Dorcoceras hygrometr... 330 e-109
XP_016710240.1 PREDICTED: putative dual specificity protein phos... 329 e-109
XP_012843528.1 PREDICTED: putative dual specificity protein phos... 329 e-108
XP_010258798.1 PREDICTED: putative dual specificity protein phos... 328 e-108
XP_015055742.1 PREDICTED: putative dual specificity protein phos... 327 e-108
XP_004248525.1 PREDICTED: putative dual specificity protein phos... 327 e-108
>XP_017216574.1 PREDICTED: putative dual specificity protein phosphatase DSP8
[Daucus carota subsp. sativus] KZM87971.1 hypothetical
protein DCAR_025072 [Daucus carota subsp. sativus]
Length = 307
Score = 481 bits (1238), Expect = e-169
Identities = 236/274 (86%), Positives = 248/274 (90%)
Frame = +1
Query: 1 PTLLYNVVRNKIQSDFRWWDRVDEFILLGAVPFPTDVQRLKELGVHGVVTLNEPYETLVS 180
PTLLYNVVRNKIQ FRWWDRVDEFILLGAVPFP DVQRLKELGVHGVVTLNEPYETLVS
Sbjct: 35 PTLLYNVVRNKIQPQFRWWDRVDEFILLGAVPFPADVQRLKELGVHGVVTLNEPYETLVS 94
Query: 181 TSLYHAHDINHLVIPTRDYLFAPCYGDIQQAVDFIHNNASRGKTTYVHCKAGRGRSTTVV 360
TSLY AH INHLVIPTRDYLFAPCYGDI+QAVDFIHNNASRGKTTYVHCKAGRGRSTTVV
Sbjct: 95 TSLYDAHGINHLVIPTRDYLFAPCYGDIRQAVDFIHNNASRGKTTYVHCKAGRGRSTTVV 154
Query: 361 LCYLVEHKQMTPIVAYEYVRSIRPRVRLASSQWQAVQGYYQCLTERCICTESSPDTKLSC 540
LCYLVEHKQMTP VAYEYVRSIRPRVRLASSQWQAVQ YYQ ER ICT SS DT LSC
Sbjct: 155 LCYLVEHKQMTPTVAYEYVRSIRPRVRLASSQWQAVQDYYQRWKERYICT-SSTDTTLSC 213
Query: 541 SEETNFEAFDDNCMVLVSESDIEGYEMSLNRDVACSMLTELSVACRLQLTSQAAITRLLC 720
SE+TNFEAFDD+CMVLVS+SDIEGYE+SLN +V + LTELSVACR+QLTSQAAI RL C
Sbjct: 214 SEKTNFEAFDDSCMVLVSKSDIEGYELSLNSNVVYNKLTELSVACRVQLTSQAAIARLSC 273
Query: 721 MWPRVHGHKFWRKKLESFVKNDQPSRIRFDLQVC 822
W R+HGHKFWRKKL+S + NDQ S +RFDLQVC
Sbjct: 274 TWLRIHGHKFWRKKLQSSIGNDQQSSVRFDLQVC 307
>XP_011089735.1 PREDICTED: putative dual specificity protein phosphatase DSP8
[Sesamum indicum] XP_011089744.1 PREDICTED: putative
dual specificity protein phosphatase DSP8 [Sesamum
indicum] XP_011089753.1 PREDICTED: putative dual
specificity protein phosphatase DSP8 [Sesamum indicum]
XP_011089760.1 PREDICTED: putative dual specificity
protein phosphatase DSP8 [Sesamum indicum]
XP_011089768.1 PREDICTED: putative dual specificity
protein phosphatase DSP8 [Sesamum indicum]
Length = 324
Score = 352 bits (904), Expect = e-118
Identities = 185/277 (66%), Positives = 213/277 (76%), Gaps = 4/277 (1%)
Frame = +1
Query: 1 PTLLYNVVRNKIQSDFRWWDRVDEFILLGAVPFPTDVQRLKELGVHGVVTLNEPYETLVS 180
PTLLYNVVRNKIQS+FRWWDRVD+++LLGAVPFPTDV RLK LGV GVVTLNEPYETLV
Sbjct: 47 PTLLYNVVRNKIQSEFRWWDRVDQYVLLGAVPFPTDVPRLKALGVGGVVTLNEPYETLVP 106
Query: 181 TSLYHAHDINHLVIPTRDYLFAPCYGDIQQAVDFIHNNASRGKTTYVHCKAGRGRSTTVV 360
TSLYH H I HLVIPTRDYLFAP +GDI QAVDFIH NA GKTTYVHCKAGRGRSTT+V
Sbjct: 107 TSLYHDHSIEHLVIPTRDYLFAPSHGDICQAVDFIHGNALCGKTTYVHCKAGRGRSTTIV 166
Query: 361 LCYLVEHKQMTPIVAYEYVRSIRPRVRLASSQWQAVQGYYQCL--TERCICTESSPDTKL 534
LCYLV+HKQMTP AYEYVRSIRPRV LASSQ QAVQ YY L E C ++ P L
Sbjct: 167 LCYLVKHKQMTPEAAYEYVRSIRPRVLLASSQRQAVQDYYHGLEKPEAETCMDNLPRKAL 226
Query: 535 SCSEETNFEAFDDNCMVLVSESDIEGYEMSLNRDVA-CSMLTELSVACRLQLTSQAAITR 711
S + + E FDDN +VL++ESD++GYE S + A +MLTEL++ACR+ SQAA +R
Sbjct: 227 SFPVQADPEDFDDNSLVLITESDLDGYEESPDSGSAGQNMLTELNLACRVHFASQAAFSR 286
Query: 712 LLCMWPRVH-GHKFWRKKLESFVKNDQPSRIRFDLQV 819
L C+W R K RKKL+S V+N++ I D+ V
Sbjct: 287 LSCLWLRSQTDQKVSRKKLQSSVRNNRLGNIGVDIHV 323
>XP_006464441.1 PREDICTED: putative dual specificity protein phosphatase DSP8
isoform X2 [Citrus sinensis] KDO85554.1 hypothetical
protein CISIN_1g020371mg [Citrus sinensis]
Length = 323
Score = 348 bits (893), Expect = e-116
Identities = 182/285 (63%), Positives = 212/285 (74%), Gaps = 12/285 (4%)
Frame = +1
Query: 1 PTLLYNVVRNKIQSDFRWWDRVDEFILLGAVPFPTDVQRLKELGVHGVVTLNEPYETLVS 180
PTLLYNVVRNKIQS+FRWWDRVD+FI+LGAVPFP DV RLKELGV GVVTLNE YETLV
Sbjct: 47 PTLLYNVVRNKIQSEFRWWDRVDQFIILGAVPFPADVLRLKELGVSGVVTLNESYETLVP 106
Query: 181 TSLYHAHDINHLVIPTRDYLFAPCYGDIQQAVDFIHNNASRGKTTYVHCKAGRGRSTTVV 360
TSLYH H+I+HLVIPTRDYLFAP + DI QAVDFIH NAS GKTTYVHCKAGRGRSTT+V
Sbjct: 107 TSLYHDHNIDHLVIPTRDYLFAPSFADICQAVDFIHENASLGKTTYVHCKAGRGRSTTIV 166
Query: 361 LCYLVEHKQMTPIVAYEYVRSIRPRVRLASSQWQAVQGYY----------QCLTERCICT 510
LCYLVEH+QM P AYEYVRSIRPRV LASSQWQAVQ YY C+T R
Sbjct: 167 LCYLVEHRQMAPEAAYEYVRSIRPRVLLASSQWQAVQDYYLQKVKKIGNSDCITLR---- 222
Query: 511 ESSPDTKLSCSEETNFEAFDDNCMVLVSESDIEGYEMSLNRDVACS-MLTELSVACRLQL 687
T L + + E+FDD +V+V+E+D++GY+ S + VA + L ELS+AC++Q
Sbjct: 223 -----TSLPFPVDQDSESFDDGSVVVVTETDLDGYDASYDSGVAGNHRLAELSLACKVQF 277
Query: 688 TSQAAITRLLCMWPR-VHGHKFWRKKLESFVKNDQPSRIRFDLQV 819
SQ+AI RL C+WPR HK R+KL + V NDQ + D+ V
Sbjct: 278 ASQSAIARLSCLWPRWQEDHKTSRQKLRNSVGNDQLGSLSVDIWV 322
>XP_011031866.1 PREDICTED: putative dual specificity protein phosphatase DSP8
[Populus euphratica]
Length = 319
Score = 345 bits (884), Expect = e-115
Identities = 180/281 (64%), Positives = 210/281 (74%), Gaps = 7/281 (2%)
Frame = +1
Query: 1 PTLLYNVVRNKIQSDFRWWDRVDEFILLGAVPFPTDVQRLKELGVHGVVTLNEPYETLVS 180
PTLLYNV+RNKIQS+FRWWDRVD+FILLGAVPFPTDV RLK LGV GVVTLNE YETLV
Sbjct: 39 PTLLYNVLRNKIQSEFRWWDRVDQFILLGAVPFPTDVPRLKTLGVSGVVTLNESYETLVP 98
Query: 181 TSLYHAHDINHLVIPTRDYLFAPCYGDIQQAVDFIHNNASRGKTTYVHCKAGRGRSTTVV 360
TSLYHAHDI+HLVIPTRDYLFAP + DI QAVDFIH NAS GKTTYVHCKAGRGRSTT+V
Sbjct: 99 TSLYHAHDIDHLVIPTRDYLFAPLFSDICQAVDFIHENASLGKTTYVHCKAGRGRSTTIV 158
Query: 361 LCYLVEHKQMTPIVAYEYVRSIRPRVRLASSQWQAVQGYYQCLTER-----CICTESSPD 525
LCYLVEH+ MTP AYE+VRSIRPRV LASSQWQAVQ YY ++ C+ + + D
Sbjct: 159 LCYLVEHRHMTPKSAYEHVRSIRPRVLLASSQWQAVQDYYLLKVKKISIPGCMIIQKALD 218
Query: 526 TKLSCSEETNFEAFDDNCMVLVSESDIEGYEMSLNRDVACS-MLTELSVACRLQLTSQAA 702
+ + AFDD VLV+ESD++GY+ + V + ML E S+AC++Q SQAA
Sbjct: 219 LPTEEDRKQDTAAFDDGSAVLVTESDLDGYDATCAFGVVGNDMLREPSLACKVQFASQAA 278
Query: 703 ITRLLCMWPRVH-GHKFWRKKLESFVKNDQPSRIRFDLQVC 822
I+RL C+W K KKLES ++ Q S I D++VC
Sbjct: 279 ISRLSCLWLGYQPDQKSSTKKLESSIRASQLSSITVDIRVC 319
>XP_015388085.1 PREDICTED: putative dual specificity protein phosphatase DSP8
isoform X1 [Citrus sinensis] KDO85555.1 hypothetical
protein CISIN_1g020371mg [Citrus sinensis]
Length = 327
Score = 342 bits (878), Expect = e-114
Identities = 182/289 (62%), Positives = 212/289 (73%), Gaps = 16/289 (5%)
Frame = +1
Query: 1 PTLLYNVVRNKIQSDFRWWDRVDEFILLGAVPFPTDVQRLKELGVHGVVTLNEPYETLVS 180
PTLLYNVVRNKIQS+FRWWDRVD+FI+LGAVPFP DV RLKELGV GVVTLNE YETLV
Sbjct: 47 PTLLYNVVRNKIQSEFRWWDRVDQFIILGAVPFPADVLRLKELGVSGVVTLNESYETLVP 106
Query: 181 TSLYHAHDINHLVIPTRDYLFAPCYGDIQQAVDFIHNNASRGKTTYVHCKAGRGRSTTVV 360
TSLYH H+I+HLVIPTRDYLFAP + DI QAVDFIH NAS GKTTYVHCKAGRGRSTT+V
Sbjct: 107 TSLYHDHNIDHLVIPTRDYLFAPSFADICQAVDFIHENASLGKTTYVHCKAGRGRSTTIV 166
Query: 361 LCYL----VEHKQMTPIVAYEYVRSIRPRVRLASSQWQAVQGYY----------QCLTER 498
LCYL VEH+QM P AYEYVRSIRPRV LASSQWQAVQ YY C+T R
Sbjct: 167 LCYLFSLQVEHRQMAPEAAYEYVRSIRPRVLLASSQWQAVQDYYLQKVKKIGNSDCITLR 226
Query: 499 CICTESSPDTKLSCSEETNFEAFDDNCMVLVSESDIEGYEMSLNRDVACS-MLTELSVAC 675
T L + + E+FDD +V+V+E+D++GY+ S + VA + L ELS+AC
Sbjct: 227 ---------TSLPFPVDQDSESFDDGSVVVVTETDLDGYDASYDSGVAGNHRLAELSLAC 277
Query: 676 RLQLTSQAAITRLLCMWPR-VHGHKFWRKKLESFVKNDQPSRIRFDLQV 819
++Q SQ+AI RL C+WPR HK R+KL + V NDQ + D+ V
Sbjct: 278 KVQFASQSAIARLSCLWPRWQEDHKTSRQKLRNSVGNDQLGSLSVDIWV 326
>XP_006375113.1 hypothetical protein POPTR_0014s04500g [Populus trichocarpa]
ERP52910.1 hypothetical protein POPTR_0014s04500g
[Populus trichocarpa]
Length = 319
Score = 341 bits (874), Expect = e-113
Identities = 178/281 (63%), Positives = 209/281 (74%), Gaps = 7/281 (2%)
Frame = +1
Query: 1 PTLLYNVVRNKIQSDFRWWDRVDEFILLGAVPFPTDVQRLKELGVHGVVTLNEPYETLVS 180
PTLLYNV+RNKIQS+FRWWDRVD+FILLGAVPFPTDV RL+ LGV GVVTLNE YETLV
Sbjct: 39 PTLLYNVLRNKIQSEFRWWDRVDQFILLGAVPFPTDVPRLQALGVSGVVTLNESYETLVP 98
Query: 181 TSLYHAHDINHLVIPTRDYLFAPCYGDIQQAVDFIHNNASRGKTTYVHCKAGRGRSTTVV 360
TSLYHAHDI+HLVIPTRDYLFAP + DI QAVDFIH NAS GKTTYVHCKAGRGRSTT+V
Sbjct: 99 TSLYHAHDIDHLVIPTRDYLFAPLFSDICQAVDFIHENASLGKTTYVHCKAGRGRSTTIV 158
Query: 361 LCYLVEHKQMTPIVAYEYVRSIRPRVRLASSQWQAVQGYYQCLTER-----CICTESSPD 525
LCYLVEH+ MTP AYE+VRSIRPRV LASSQWQAVQ YY ++ C+ + + D
Sbjct: 159 LCYLVEHRHMTPKSAYEHVRSIRPRVLLASSQWQAVQDYYLLKVKKITIPGCMIIQKALD 218
Query: 526 TKLSCSEETNFEAFDDNCMVLVSESDIEGYEMSLNRDVACS-MLTELSVACRLQLTSQAA 702
+ + AFDD VLV+ESD++GY+ + V + ML E S+AC++Q SQAA
Sbjct: 219 LPTKEDGKQDTAAFDDGSAVLVTESDLDGYDATCALGVVGNDMLREPSLACKVQFASQAA 278
Query: 703 ITRLLCMWPRVH-GHKFWRKKLESFVKNDQPSRIRFDLQVC 822
I+RL C+W K KKL S ++ Q S I D++VC
Sbjct: 279 ISRLSCLWLGYQPDQKSSTKKLRSSIRASQLSSITVDIRVC 319
>XP_019188798.1 PREDICTED: putative dual specificity protein phosphatase DSP8
[Ipomoea nil]
Length = 307
Score = 339 bits (870), Expect = e-113
Identities = 180/269 (66%), Positives = 205/269 (76%), Gaps = 6/269 (2%)
Frame = +1
Query: 1 PTLLYNVVRNKIQSDFRWWDRVDEFILLGAVPFPTDVQRLKELGVHGVVTLNEPYETLVS 180
PTLLYNV+RNK+Q++FRWWDRVDEFILLGAVPFP DV LK LGV GVVTLNEPYETLV
Sbjct: 37 PTLLYNVLRNKVQAEFRWWDRVDEFILLGAVPFPADVPHLKALGVFGVVTLNEPYETLVP 96
Query: 181 TSLYHAHDINHLVIPTRDYLFAPCYGDIQQAVDFIHNNASRGKTTYVHCKAGRGRSTTVV 360
TSLY H INHLVIPTRDYLFAP + DI QAVDFI NAS GKTTYVHCKAGRGRSTTVV
Sbjct: 97 TSLYRDHGINHLVIPTRDYLFAPSFLDISQAVDFIQGNASCGKTTYVHCKAGRGRSTTVV 156
Query: 361 LCYLVEHKQMTPIVAYEYVRSIRPRVRLASSQWQAVQGYYQCLTERCICTESSPDTKLSC 540
LCYLV+HKQMTP AY+YVRSIRPRV LASSQWQAVQ YY R TESS DT L
Sbjct: 157 LCYLVKHKQMTPEAAYDYVRSIRPRVSLASSQWQAVQEYYY----RVRKTESSADTLLEK 212
Query: 541 S----EETNFEAFDDNCMVLVSESDIEGYEMSLNRDV-ACSMLTELSVACRLQLTSQAAI 705
+ + AFDD+ +VLV+ESD++GY+ +L D+ ++L EL++ACR+Q QAAI
Sbjct: 213 ALIRPSNGDLAAFDDDSLVLVTESDLDGYDENLECDLGGNNVLAELNLACRVQFAGQAAI 272
Query: 706 TRLLCMWPRVH-GHKFWRKKLESFVKNDQ 789
RL C+W R + G K KKLE V +Q
Sbjct: 273 ARLSCLWLRCNPGQKTSGKKLEGSVTQNQ 301
>XP_011017326.1 PREDICTED: putative dual specificity protein phosphatase DSP8
isoform X1 [Populus euphratica]
Length = 319
Score = 335 bits (859), Expect = e-111
Identities = 173/280 (61%), Positives = 208/280 (74%), Gaps = 7/280 (2%)
Frame = +1
Query: 1 PTLLYNVVRNKIQSDFRWWDRVDEFILLGAVPFPTDVQRLKELGVHGVVTLNEPYETLVS 180
PTLLYNV+RNKI+S+F WWDRVD+FILLGAVPFPTDV+RLK+LGV GVVTLNE YETLV
Sbjct: 39 PTLLYNVLRNKIESEFHWWDRVDQFILLGAVPFPTDVRRLKKLGVSGVVTLNESYETLVP 98
Query: 181 TSLYHAHDINHLVIPTRDYLFAPCYGDIQQAVDFIHNNASRGKTTYVHCKAGRGRSTTVV 360
TSLYHAHDI+HLVIPTRDYLFAP + DI QAVDFIH NAS G+TTYVHCKAGRGRSTT+V
Sbjct: 99 TSLYHAHDIDHLVIPTRDYLFAPSFTDICQAVDFIHKNASLGRTTYVHCKAGRGRSTTIV 158
Query: 361 LCYLVEHKQMTPIVAYEYVRSIRPRVRLASSQWQAVQGYYQCLTER-----CICTESSPD 525
LCYLVEH+ M P AYE+VRSIRPRV L SSQWQAVQ YY ++ C T+ + D
Sbjct: 159 LCYLVEHRHMLPKAAYEHVRSIRPRVLLVSSQWQAVQDYYLLKVKKTSVPGCTVTQKALD 218
Query: 526 TKLSCSEETNFEAFDDNCMVLVSESDIEGYEMSLNRD-VACSMLTELSVACRLQLTSQAA 702
+ E+ + +FDD VLV+ES+++GY+ S + V ML E +AC++Q S+AA
Sbjct: 219 LPIRGDEKQDTASFDDGSAVLVTESELDGYDASCDSGLVGNDMLAEHGLACKVQFASRAA 278
Query: 703 ITRLLCMWPRVH-GHKFWRKKLESFVKNDQPSRIRFDLQV 819
I+RL C+W H K KK S + +Q S I D+QV
Sbjct: 279 ISRLSCLWLGCHPDQKSSMKKPGSSIGGNQLSSIAADIQV 318
>XP_002301226.2 hypothetical protein POPTR_0002s13750g [Populus trichocarpa]
EEE80499.2 hypothetical protein POPTR_0002s13750g
[Populus trichocarpa]
Length = 319
Score = 333 bits (855), Expect = e-110
Identities = 173/279 (62%), Positives = 206/279 (73%), Gaps = 7/279 (2%)
Frame = +1
Query: 1 PTLLYNVVRNKIQSDFRWWDRVDEFILLGAVPFPTDVQRLKELGVHGVVTLNEPYETLVS 180
PTLLYNV+RNKI+S+F WWDRVD+FILLGAVPFPTDV+RLK+LGV GVVTLNE YETLV
Sbjct: 39 PTLLYNVLRNKIESEFHWWDRVDQFILLGAVPFPTDVRRLKKLGVSGVVTLNESYETLVP 98
Query: 181 TSLYHAHDINHLVIPTRDYLFAPCYGDIQQAVDFIHNNASRGKTTYVHCKAGRGRSTTVV 360
TSLYHAHDI+HLVIPTRDYLFAP + DI QAVDFIH NAS GKTTYVHCKAGRGRSTT+V
Sbjct: 99 TSLYHAHDIDHLVIPTRDYLFAPSFTDICQAVDFIHENASLGKTTYVHCKAGRGRSTTIV 158
Query: 361 LCYLVEHKQMTPIVAYEYVRSIRPRVRLASSQWQAVQGYYQCLTER-----CICTESSPD 525
LCYLVEH+ M P AYE+VRSIRPRV L SSQWQAVQ YY ++ C T+ + D
Sbjct: 159 LCYLVEHRHMLPKAAYEHVRSIRPRVLLVSSQWQAVQDYYLLKVKKTSVPGCTVTQKALD 218
Query: 526 TKLSCSEETNFEAFDDNCMVLVSESDIEGYEMSLNRD-VACSMLTELSVACRLQLTSQAA 702
E+ + +FDD VLV+ES+++GY+ S + V ML E +AC++Q S+AA
Sbjct: 219 LPTRGDEKQDTASFDDGSAVLVTESELDGYDASCDSGLVGNDMLAEHGLACKVQFASRAA 278
Query: 703 ITRLLCMWPRVH-GHKFWRKKLESFVKNDQPSRIRFDLQ 816
I+RL C+W H K KK S + +Q S I D+Q
Sbjct: 279 ISRLSCLWLGCHPDQKSSMKKPGSSIGGNQLSSIAADIQ 317
>XP_010275392.1 PREDICTED: putative dual specificity protein phosphatase DSP8
[Nelumbo nucifera]
Length = 327
Score = 333 bits (853), Expect = e-110
Identities = 177/283 (62%), Positives = 207/283 (73%), Gaps = 10/283 (3%)
Frame = +1
Query: 1 PTLLYNVVRNKIQSDFRWWDRVDEFILLGAVPFPTDVQRLKELGVHGVVTLNEPYETLVS 180
PTLLYNVVRNKIQS+FRWWDRVDEF+LLGAVPFPTDV LK+LGV GV+TLNEPYETLV
Sbjct: 46 PTLLYNVVRNKIQSEFRWWDRVDEFVLLGAVPFPTDVPHLKQLGVRGVITLNEPYETLVP 105
Query: 181 TSLYHAHDINHLVIPTRDYLFAPCYGDIQQAVDFIHNNASRGKTTYVHCKAGRGRSTTVV 360
TSLYHAH I HLVIPTRDYLFAP +GDI +AVDFIH NAS GKTTYVHCKAGRGRSTT+V
Sbjct: 106 TSLYHAHGIEHLVIPTRDYLFAPSFGDICKAVDFIHKNASSGKTTYVHCKAGRGRSTTIV 165
Query: 361 LCYLVEHKQMTPIVAYEYVRSIRPRVRLASSQWQAVQGYYQCLTERCICTE---SSPDTK 531
LCYLVEHKQM+P AYEYVRSIR RV LASSQWQAVQ YY +R + SP K
Sbjct: 166 LCYLVEHKQMSPDTAYEYVRSIRQRVLLASSQWQAVQEYYHQKVKRTAVSSFWLRSPIVK 225
Query: 532 LSCSEETNFEAFDDNCMVLVSESDIEGYEMSLNRDVACSML-TELSVACRLQLTSQAAIT 708
S + + AFDD +V+V+++D++GY+ S +V + + ELS+ R+Q SQAA+
Sbjct: 226 TS-THPRDIMAFDDGSVVVVTKADLDGYDESNGSNVVGNEIWAELSLVYRVQFASQAALA 284
Query: 709 RLLCMWPRVH------GHKFWRKKLESFVKNDQPSRIRFDLQV 819
RL C+W R H G K R+ S V DQ + D+ V
Sbjct: 285 RLSCLWLRCHARQKISGEKLGRESSYS-VGADQMGGLDVDIHV 326
>XP_012083664.1 PREDICTED: putative dual specificity protein phosphatase DSP8
[Jatropha curcas] KDP28828.1 hypothetical protein
JCGZ_14599 [Jatropha curcas]
Length = 331
Score = 332 bits (850), Expect = e-109
Identities = 172/280 (61%), Positives = 202/280 (72%), Gaps = 7/280 (2%)
Frame = +1
Query: 1 PTLLYNVVRNKIQSDFRWWDRVDEFILLGAVPFPTDVQRLKELGVHGVVTLNEPYETLVS 180
PTLLYNV+RNKIQS+FRWWDRVDEFILLGAVPFPTDV RLKELGV GVVTLNEPYETLV
Sbjct: 51 PTLLYNVLRNKIQSEFRWWDRVDEFILLGAVPFPTDVPRLKELGVSGVVTLNEPYETLVP 110
Query: 181 TSLYHAHDINHLVIPTRDYLFAPCYGDIQQAVDFIHNNASRGKTTYVHCKAGRGRSTTVV 360
TSLYHAHDI HLVIPTRDY+FAP + DI QAVDFIH+NAS GK TYVHCKAGRGRSTT+V
Sbjct: 111 TSLYHAHDIVHLVIPTRDYMFAPAFADICQAVDFIHDNASLGKKTYVHCKAGRGRSTTIV 170
Query: 361 LCYLVEHKQMTPIVAYEYVRSIRPRVRLASSQWQAVQGYY-----QCLTERCICTESSPD 525
LCYLV+H+ MTP AYEYVRS+RPRV LASSQW AVQ YY + R + + D
Sbjct: 171 LCYLVQHRDMTPEAAYEYVRSVRPRVLLASSQWHAVQDYYLLKVRETCNPRFMTARKALD 230
Query: 526 TKLSCSEETNFEAF-DDNCMVLVSESDIEGYEMSLNRDVACS-MLTELSVACRLQLTSQA 699
+ N E DD+ V+V ESD++GY+ + + ML ELS CR+ QA
Sbjct: 231 CLTKEHAKQNIEDHDDDDTFVMVMESDLDGYDFECELGIVDNKMLGELSPVCRVHFAGQA 290
Query: 700 AITRLLCMWPRVHGHKFWRKKLESFVKNDQPSRIRFDLQV 819
AI+RL C+W H ++ +K S V +D+ + D+QV
Sbjct: 291 AISRLSCLWLGCHANRKPSEKPGSSVGDDRLRSLTVDIQV 330
>CDP09450.1 unnamed protein product [Coffea canephora]
Length = 324
Score = 330 bits (847), Expect = e-109
Identities = 177/277 (63%), Positives = 206/277 (74%), Gaps = 4/277 (1%)
Frame = +1
Query: 1 PTLLYNVVRNKIQSDFRWWDRVDEFILLGAVPFPTDVQRLKELGVHGVVTLNEPYETLVS 180
PTLLYNVVRNKI+S+F WWD+VDEFILLGAVPFP DV RLKELGV GVVTLNEPYETLV
Sbjct: 47 PTLLYNVVRNKIESEFHWWDKVDEFILLGAVPFPADVPRLKELGVCGVVTLNEPYETLVP 106
Query: 181 TSLYHAHDINHLVIPTRDYLFAPCYGDIQQAVDFIHNNASRGKTTYVHCKAGRGRSTTVV 360
TSLYH HDI+HLVIPTRDYLFAP DI QAVDFIH NASRGKTTYVHCKAGRGRSTT+V
Sbjct: 107 TSLYHDHDIDHLVIPTRDYLFAPTNDDICQAVDFIHGNASRGKTTYVHCKAGRGRSTTIV 166
Query: 361 LCYLVEHKQMTPIVAYEYVRSIRPRVRLASSQWQAVQGYYQCL--TERCI-CTESSPDTK 531
LCY+V+HK MTP VAYE+VRSIRPRV LASSQ QAV+ +Y L TE I + S +
Sbjct: 167 LCYMVKHKDMTPKVAYEHVRSIRPRVLLASSQRQAVENFYIHLKRTENDISVVDRSLISI 226
Query: 532 LSCSEETNFEAFDDNCMVLVSESDIEGYEMSLNRD-VACSMLTELSVACRLQLTSQAAIT 708
L +F AFDD +VLV++SD++GY+ S V S++ E ++ACR Q SQAA+
Sbjct: 227 LGFPATLDFSAFDDGSVVLVTKSDLDGYDESHESGVVGKSIMAESNLACRFQFVSQAAVA 286
Query: 709 RLLCMWPRVHGHKFWRKKLESFVKNDQPSRIRFDLQV 819
RL C+W R + KKL S + + Q I D+ V
Sbjct: 287 RLSCLWVRSQTGEKVSKKLGSSMTSSQLQSIGVDIHV 323
>XP_017615966.1 PREDICTED: putative dual specificity protein phosphatase DSP8
[Gossypium arboreum]
Length = 314
Score = 330 bits (846), Expect = e-109
Identities = 173/262 (66%), Positives = 200/262 (76%), Gaps = 5/262 (1%)
Frame = +1
Query: 1 PTLLYNVVRNKIQSDFRWWDRVDEFILLGAVPFPTDVQRLKELGVHGVVTLNEPYETLVS 180
PTLLYN+VRNKIQS+FRWWDRVDEFILLGAVPFP DV RLK+LGV GVVTLNEPYETLV
Sbjct: 44 PTLLYNLVRNKIQSEFRWWDRVDEFILLGAVPFPADVPRLKDLGVSGVVTLNEPYETLVP 103
Query: 181 TSLYHAHDINHLVIPTRDYLFAPCYGDIQQAVDFIHNNASRGKTTYVHCKAGRGRSTTVV 360
TSLYHAH+I+HLVIPTRDYLFAP + DI QAVDFIH NAS GKTTYVHCKAGRGRSTT+V
Sbjct: 104 TSLYHAHNIHHLVIPTRDYLFAPSFADICQAVDFIHENASVGKTTYVHCKAGRGRSTTIV 163
Query: 361 LCYLVEHKQMTPIVAYEYVRSIRPRVRLASSQWQAVQGYY-QCLTERCICTESSP---DT 528
LCYLVEH+ MTP AYEYVRSIRPRV LA +Q QAVQ YY + IC +S
Sbjct: 164 LCYLVEHRHMTPDAAYEYVRSIRPRVLLAPAQQQAVQDYYLHKVNNNGICGDSCSMIIKK 223
Query: 529 KLSCSEETNFEAFDDNCMVLVSESDIEGYEMSLNRDVACS-MLTELSVACRLQLTSQAAI 705
L + + + FDD +V+V+ESD++GY+ S + DV + ML E S+A RLQ SQAA+
Sbjct: 224 TLVVAAKQDTAVFDDGSVVVVTESDLDGYDASFDTDVLSNKMLDEGSLAGRLQFASQAAM 283
Query: 706 TRLLCMWPRVHGHKFWRKKLES 771
TRL C+W R + +KL S
Sbjct: 284 TRLSCLWLRCRAEQKASRKLLS 305
>XP_012489699.1 PREDICTED: putative dual specificity protein phosphatase DSP8
[Gossypium raimondii] KJB40984.1 hypothetical protein
B456_007G086300 [Gossypium raimondii]
Length = 314
Score = 329 bits (844), Expect = e-109
Identities = 173/262 (66%), Positives = 199/262 (75%), Gaps = 5/262 (1%)
Frame = +1
Query: 1 PTLLYNVVRNKIQSDFRWWDRVDEFILLGAVPFPTDVQRLKELGVHGVVTLNEPYETLVS 180
PTLLYN+VRNKIQS+FRWWDRVDEFILLGAVPFP DV RLK+LGV GVVTLNEPYETLV
Sbjct: 44 PTLLYNLVRNKIQSEFRWWDRVDEFILLGAVPFPADVPRLKDLGVSGVVTLNEPYETLVP 103
Query: 181 TSLYHAHDINHLVIPTRDYLFAPCYGDIQQAVDFIHNNASRGKTTYVHCKAGRGRSTTVV 360
TSLYHAH+I+HLVIPTRDYLFAP + DI QAVDFIH NAS GKTTYVHCKAGRGRSTT+V
Sbjct: 104 TSLYHAHNIHHLVIPTRDYLFAPSFADICQAVDFIHENASVGKTTYVHCKAGRGRSTTIV 163
Query: 361 LCYLVEHKQMTPIVAYEYVRSIRPRVRLASSQWQAVQGYY-QCLTERCICTESSP---DT 528
LCYLVEH+ MTP AYEYVRSIRPRV LA +Q QAVQ YY + IC +S
Sbjct: 164 LCYLVEHRHMTPDAAYEYVRSIRPRVLLAPAQQQAVQDYYLHKVNNNGICGDSCSMIIKK 223
Query: 529 KLSCSEETNFEAFDDNCMVLVSESDIEGYEMSLNRDVACS-MLTELSVACRLQLTSQAAI 705
L + + FDD +V+V+ESD++GY+ S + DV + ML E S+A RLQ SQAA+
Sbjct: 224 TLVVPAKQDTAVFDDGSVVVVTESDLDGYDASFDTDVLSNEMLDEGSLAGRLQFASQAAM 283
Query: 706 TRLLCMWPRVHGHKFWRKKLES 771
TRL C+W R + +KL S
Sbjct: 284 TRLSCLWLRCRAEQKASRKLLS 305
>KZV45079.1 hypothetical protein F511_29400 [Dorcoceras hygrometricum]
Length = 325
Score = 330 bits (845), Expect = e-109
Identities = 171/280 (61%), Positives = 208/280 (74%), Gaps = 7/280 (2%)
Frame = +1
Query: 1 PTLLYNVVRNKIQSDFRWWDRVDEFILLGAVPFPTDVQRLKELGVHGVVTLNEPYETLVS 180
PTLLYNV+RNKIQS+FRWWDRVDEFILLGAVPFPTDV L+ LGV VVTLNEPYETLV
Sbjct: 47 PTLLYNVIRNKIQSEFRWWDRVDEFILLGAVPFPTDVPCLRALGVGAVVTLNEPYETLVP 106
Query: 181 TSLYHAHDINHLVIPTRDYLFAPCYGDIQQAVDFIHNNASRGKTTYVHCKAGRGRSTTVV 360
TSLYH +++ HLVIPTRDYLFAP YGDI QAV+FIH+N GKTTYVHCKAGRGRSTT+V
Sbjct: 107 TSLYHDYNMEHLVIPTRDYLFAPAYGDICQAVEFIHSNTLSGKTTYVHCKAGRGRSTTIV 166
Query: 361 LCYLVEHKQMTPIVAYEYVRSIRPRVRLASSQWQAVQGYYQCLTERCI--CTESSPDTKL 534
LCYLV++K+MTP A++YVRSIRPRV LASSQWQAVQ Y+ L + + CT+ L
Sbjct: 167 LCYLVKYKKMTPDAAFQYVRSIRPRVLLASSQWQAVQDYHIRLKKPAVKSCTDHLQSRSL 226
Query: 535 SCSEETNFEAFDDNCMVLVSESDIEGYEMSLNRDV---ACSMLTELSVACRLQLTSQAAI 705
S + + E FD +V+V ESD++GYE NRD +M+TE+++ACR+Q QAAI
Sbjct: 227 SFPLDVDLEDFDYGSIVVVDESDLDGYEE--NRDSNPGGRNMMTEVNLACRVQFVRQAAI 284
Query: 706 TRLLCMWPRVHGH--KFWRKKLESFVKNDQPSRIRFDLQV 819
RL C+W R H + RKK+ + V + Q + D+ V
Sbjct: 285 ARLSCLWLRTHSEQKEVTRKKMGNSVGSSQIGSLGVDVHV 324
>XP_016710240.1 PREDICTED: putative dual specificity protein phosphatase DSP8
[Gossypium hirsutum]
Length = 314
Score = 329 bits (843), Expect = e-109
Identities = 172/262 (65%), Positives = 199/262 (75%), Gaps = 5/262 (1%)
Frame = +1
Query: 1 PTLLYNVVRNKIQSDFRWWDRVDEFILLGAVPFPTDVQRLKELGVHGVVTLNEPYETLVS 180
PTLLYN+VRNKIQS+FRWWDRVDEFILLGAVPFP DV R K+LGV GVVTLNEPYETLV
Sbjct: 44 PTLLYNLVRNKIQSEFRWWDRVDEFILLGAVPFPADVPRWKDLGVSGVVTLNEPYETLVP 103
Query: 181 TSLYHAHDINHLVIPTRDYLFAPCYGDIQQAVDFIHNNASRGKTTYVHCKAGRGRSTTVV 360
TSLYHAH+I+HLV+PTRDYLFAP + DI QAVDFIH NAS GKTTYVHCKAGRGRSTT+V
Sbjct: 104 TSLYHAHNIHHLVMPTRDYLFAPSFADICQAVDFIHENASVGKTTYVHCKAGRGRSTTIV 163
Query: 361 LCYLVEHKQMTPIVAYEYVRSIRPRVRLASSQWQAVQGYY-QCLTERCICTESSP---DT 528
LCYLVEH+ MTP AYEYVRSIRPRV LA +Q QAVQ YY + IC +S
Sbjct: 164 LCYLVEHRHMTPDAAYEYVRSIRPRVLLAPAQQQAVQDYYLHKVNNNGICGDSCSMIIKK 223
Query: 529 KLSCSEETNFEAFDDNCMVLVSESDIEGYEMSLNRDVACS-MLTELSVACRLQLTSQAAI 705
L E+ + FDD +V+V+ESD++GY+ S + DV + ML E S+A RLQ SQAA+
Sbjct: 224 TLVVPEKQDIAVFDDGSVVVVTESDLDGYDASFDTDVLSNEMLDEGSLAGRLQFASQAAM 283
Query: 706 TRLLCMWPRVHGHKFWRKKLES 771
TRL C+W R + +KL S
Sbjct: 284 TRLSCLWLRCRAEQKASRKLLS 305
>XP_012843528.1 PREDICTED: putative dual specificity protein phosphatase DSP8
[Erythranthe guttata] EYU31907.1 hypothetical protein
MIMGU_mgv1a009822mg [Erythranthe guttata]
Length = 330
Score = 329 bits (843), Expect = e-108
Identities = 170/276 (61%), Positives = 200/276 (72%), Gaps = 10/276 (3%)
Frame = +1
Query: 1 PTLLYNVVRNKIQSDFRWWDRVDEFILLGAVPFPTDVQRLKELGVHGVVTLNEPYETLVS 180
PTLLYNVVRNKIQS+FRWWDRV EF+LLGAVPFPTDV RLK LGV GVVTLNEPYETLV
Sbjct: 46 PTLLYNVVRNKIQSEFRWWDRVHEFLLLGAVPFPTDVPRLKALGVGGVVTLNEPYETLVP 105
Query: 181 TSLYHAHDINHLVIPTRDYLFAPCYGDIQQAVDFIHNNASRGKTTYVHCKAGRGRSTTVV 360
TSLYH H I HLVI TRDYLFAP + DI QAV FIH+N GKTTYVHCKAGRGRSTT+V
Sbjct: 106 TSLYHDHCIEHLVIATRDYLFAPSHADICQAVAFIHDNEVCGKTTYVHCKAGRGRSTTIV 165
Query: 361 LCYLVEHKQMTPIVAYEYVRSIRPRVRLASSQWQAVQGYYQCLTERC----ICTESSPDT 528
LCYLV+HKQMTP AY YVR+IRPRV LA +QWQAVQ YY L ++ IC + P
Sbjct: 166 LCYLVKHKQMTPQAAYNYVRAIRPRVLLAPAQWQAVQDYYSGLMKKAEEGEICVANLPRR 225
Query: 529 KLSCSEETNFEAFDDNCMVLVSESDIEGYEMSLNRD-----VACSMLTELSVACRLQLTS 693
+ S++ E FD+N +VL++ESD++GYE + D V E+++AC++Q S
Sbjct: 226 IMGLSDKFEVEDFDENSLVLITESDLDGYEEENHPDWGKKVVVAGGGAEVNLACKVQFAS 285
Query: 694 QAAITRLLCMWPRVH-GHKFWRKKLESFVKNDQPSR 798
QAAI+R+ C W R GHK RKKL ++ R
Sbjct: 286 QAAISRISCFWLRCQTGHKVSRKKLSDTARSSSRMR 321
>XP_010258798.1 PREDICTED: putative dual specificity protein phosphatase DSP8
isoform X1 [Nelumbo nucifera]
Length = 331
Score = 328 bits (841), Expect = e-108
Identities = 171/256 (66%), Positives = 195/256 (76%), Gaps = 7/256 (2%)
Frame = +1
Query: 1 PTLLYNVVRNKIQSDFRWWDRVDEFILLGAVPFPTDVQRLKELGVHGVVTLNEPYETLVS 180
PTLLYNVVRNKIQ++FRWWD VDEFILLGAVPFPTDV RLK+LGVHGV+TLNEPYETLV
Sbjct: 47 PTLLYNVVRNKIQAEFRWWDGVDEFILLGAVPFPTDVPRLKQLGVHGVITLNEPYETLVP 106
Query: 181 TSLYHAHDINHLVIPTRDYLFAPCYGDIQQAVDFIHNNASRGKTTYVHCKAGRGRSTTVV 360
TSLYH + I HLVIPTRDYLFAP +GDI QAVDFIH NAS GKTTYVHCKAGRGRSTT+V
Sbjct: 107 TSLYHDYGIEHLVIPTRDYLFAPSFGDICQAVDFIHKNASCGKTTYVHCKAGRGRSTTIV 166
Query: 361 LCYLVEHKQMTPIVAYEYVRSIRPRVRLASSQWQAVQGYYQCLTERCICT---ESSPDTK 531
LCYLVEHKQMTP AYEYVRSIR RV LASSQWQAVQ YY+ +R + P K
Sbjct: 167 LCYLVEHKQMTPDAAYEYVRSIRRRVLLASSQWQAVQEYYRRKVKRVGRSGFWVKVPIVK 226
Query: 532 LSCSEET-NFEAFDDNCMVLVSESDIEGYEMSLNRDV---ACSMLTELSVACRLQLTSQA 699
S T + FDD +V+++ESD+EGYE + +V + +LS+A R+Q SQA
Sbjct: 227 TPGSTNTGDVVTFDDGSVVMITESDVEGYEENRESNVMGNGNEIWAQLSLAYRVQFASQA 286
Query: 700 AITRLLCMWPRVHGHK 747
A TRL C+W R H +
Sbjct: 287 AWTRLSCLWLRCHARQ 302
>XP_015055742.1 PREDICTED: putative dual specificity protein phosphatase DSP8
isoform X2 [Solanum pennellii]
Length = 312
Score = 327 bits (839), Expect = e-108
Identities = 166/253 (65%), Positives = 196/253 (77%), Gaps = 4/253 (1%)
Frame = +1
Query: 1 PTLLYNVVRNKIQSDFRWWDRVDEFILLGAVPFPTDVQRLKELGVHGVVTLNEPYETLVS 180
PTLLYNVVRNKIQ++FRWWDRVDEFILLGAVPFP DV RLK LGV GVVTLNE YETLVS
Sbjct: 48 PTLLYNVVRNKIQTEFRWWDRVDEFILLGAVPFPADVPRLKALGVAGVVTLNESYETLVS 107
Query: 181 TSLYHAHDINHLVIPTRDYLFAPCYGDIQQAVDFIHNNASRGKTTYVHCKAGRGRSTTVV 360
TSLY HDINHLVIPTRDYLFAP GDI +A++FIH+NAS GKTTYVHCKAGRGRSTT+V
Sbjct: 108 TSLYLDHDINHLVIPTRDYLFAPSDGDIDRAIEFIHSNASCGKTTYVHCKAGRGRSTTIV 167
Query: 361 LCYLVEHKQMTPIVAYEYVRSIRPRVRLASSQWQAVQGYYQCL--TERCICTESSPDTKL 534
LCYLV+HK MTP AY+YVRSIRPRV LA SQWQAVQ YY + T+ +C + + L
Sbjct: 168 LCYLVKHKDMTPETAYDYVRSIRPRVLLAPSQWQAVQAYYTRVKNTDNDVCRDDNSSKML 227
Query: 535 SCSEETNFEAFDDNCMVLVSESDIEGYEMSLNRDVACS-MLTELSVACR-LQLTSQAAIT 708
+ FDD +VLVSE D++GY+ S+ D+ + +L +L++ACR +Q+ QAAI+
Sbjct: 228 DFPAQKEVAVFDDGSVVLVSECDLDGYDESVESDLTRNEVLVDLNLACRGVQVARQAAIS 287
Query: 709 RLLCMWPRVHGHK 747
RL +W R H K
Sbjct: 288 RLSYLWLRYHSTK 300
>XP_004248525.1 PREDICTED: putative dual specificity protein phosphatase DSP8
[Solanum lycopersicum]
Length = 312
Score = 327 bits (839), Expect = e-108
Identities = 166/253 (65%), Positives = 196/253 (77%), Gaps = 4/253 (1%)
Frame = +1
Query: 1 PTLLYNVVRNKIQSDFRWWDRVDEFILLGAVPFPTDVQRLKELGVHGVVTLNEPYETLVS 180
PTLLYNVVRNKIQ++FRWWDRVDEFILLGAVPFPTDV RLK LGV GVVTLNE YETLVS
Sbjct: 48 PTLLYNVVRNKIQTEFRWWDRVDEFILLGAVPFPTDVPRLKALGVAGVVTLNESYETLVS 107
Query: 181 TSLYHAHDINHLVIPTRDYLFAPCYGDIQQAVDFIHNNASRGKTTYVHCKAGRGRSTTVV 360
TSLY HDINHLVIPTRDY FAP GDI +A++FIH+NAS GKTTYVHCKAGRGRSTT+V
Sbjct: 108 TSLYLDHDINHLVIPTRDYFFAPSDGDIDRAIEFIHSNASCGKTTYVHCKAGRGRSTTIV 167
Query: 361 LCYLVEHKQMTPIVAYEYVRSIRPRVRLASSQWQAVQGYYQCL--TERCICTESSPDTKL 534
LCYLV+HK MTP AY+YVRSIRPRV LA SQWQAVQ YY + T+ +C + + L
Sbjct: 168 LCYLVKHKDMTPEAAYDYVRSIRPRVLLAPSQWQAVQEYYTRVKNTDNDVCRDDNSSKML 227
Query: 535 SCSEETNFEAFDDNCMVLVSESDIEGYEMSLNRDVACS-MLTELSVACR-LQLTSQAAIT 708
+ FDD +VLVSE D++GY+ S+ D+ + +L +L++ACR +Q+ QAAI+
Sbjct: 228 DVPAQKEVAVFDDGSVVLVSECDLDGYDESVESDLTRNEVLVDLNLACRGVQVARQAAIS 287
Query: 709 RLLCMWPRVHGHK 747
RL +W R H K
Sbjct: 288 RLSYLWLRYHSTK 300