BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Anemarrhena21_contig00066574
         (350 letters)

Database: ./nr 
           69,698,275 sequences; 24,982,196,650 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|KIJ97544.1| hypothetical protein K443DRAFT_681432 [Laccaria a...   125   1e-26
gb|EPS97491.1| hypothetical protein FOMPIDRAFT_1024976 [Fomitops...   123   5e-26
gb|EMD40208.1| hypothetical protein CERSUDRAFT_81493 [Ceriporiop...   123   5e-26
ref|XP_001875617.1| actin depolymerizing factor [Laccaria bicolo...   122   1e-25
ref|XP_009546254.1| hypothetical protein HETIRDRAFT_383987 [Hete...   120   5e-25
gb|KJA20159.1| hypothetical protein HYPSUDRAFT_68743 [Hypholoma ...   118   2e-24
gb|KIY46739.1| cofilin [Fistulina hepatica ATCC 64428]                117   3e-24
gb|KIO25853.1| hypothetical protein M407DRAFT_243931 [Tulasnella...   116   5e-24
ref|XP_012178127.1| predicted protein [Fibroporia radiculosa] gi...   115   9e-24
ref|XP_007380427.1| hypothetical protein PUNSTDRAFT_111233 [Punc...   115   9e-24
ref|XP_007363778.1| actin depolymerizing factor [Dichomitus squa...   115   1e-23
ref|XP_007870386.1| actin depolymerizing factor, partial [Gloeop...   114   2e-23
gb|KIM41051.1| hypothetical protein M413DRAFT_445794 [Hebeloma c...   113   4e-23
ref|XP_007319810.1| hypothetical protein SERLADRAFT_392869 [Serp...   113   4e-23
ref|XP_002477528.1| predicted protein [Postia placenta Mad-698-R...   113   6e-23
gb|KIY73841.1| actin depolymerizing factor [Cylindrobasidium tor...   112   7e-23
gb|KDR83149.1| hypothetical protein GALMADRAFT_238995 [Galerina ...   112   7e-23
ref|XP_007348155.1| actin depolymerizing factor [Auricularia del...   112   9e-23
ref|XP_007845436.1| actin depolymerizing factor [Moniliophthora ...   112   1e-22
ref|XP_007304898.1| hypothetical protein STEHIDRAFT_122014 [Ster...   112   1e-22

>gb|KIJ97544.1| hypothetical protein K443DRAFT_681432 [Laccaria amethystina
           LaAM-08-1]
          Length = 138

 Score =  125 bits (314), Expect = 1e-26
 Identities = 62/100 (62%), Positives = 67/100 (67%)
 Frame = -2

Query: 301 MSSGVGVNPQCLEDXXXXXXXXXXXXXXXXXXKDNKEIVVYKTSTDTDYDSFLADLPEDE 122
           MSSGVGVNP+CLE                    DN E+VV KTS   DYD+F ADLPE  
Sbjct: 1   MSSGVGVNPECLEVFQSLKLNKKHKYIIYKLSSDNTEVVVDKTSDAQDYDTFTADLPETS 60

Query: 121 PRWAVYDFEFEKEGAGKRNKLTFYSWVPDGAKIKAKMLFA 2
           PRWAVYDF FEKEG GKRNK+TFYSW PD AKIK KML+A
Sbjct: 61  PRWAVYDFAFEKEGGGKRNKITFYSWSPDDAKIKEKMLYA 100


>gb|EPS97491.1| hypothetical protein FOMPIDRAFT_1024976 [Fomitopsis pinicola
           FP-58527 SS1]
          Length = 140

 Score =  123 bits (308), Expect = 5e-26
 Identities = 60/98 (61%), Positives = 67/98 (68%)
 Frame = -2

Query: 295 SGVGVNPQCLEDXXXXXXXXXXXXXXXXXXKDNKEIVVYKTSTDTDYDSFLADLPEDEPR 116
           SGVGVNP+CLE                    DN EI+V K S   +YD FLADLPE EPR
Sbjct: 4   SGVGVNPKCLEAYQQLKLGKKIKYIVFTLSPDNTEIIVAKQSESQNYDDFLADLPEAEPR 63

Query: 115 WAVYDFEFEKEGAGKRNKLTFYSWVPDGAKIKAKMLFA 2
           WAVYDFE+EKEGAGKRNK+TF+SW PD +KIK KMLFA
Sbjct: 64  WAVYDFEYEKEGAGKRNKITFFSWSPDDSKIKQKMLFA 101


>gb|EMD40208.1| hypothetical protein CERSUDRAFT_81493 [Ceriporiopsis subvermispora
           B]
          Length = 139

 Score =  123 bits (308), Expect = 5e-26
 Identities = 62/101 (61%), Positives = 69/101 (68%), Gaps = 1/101 (0%)
 Frame = -2

Query: 301 MSSGVGVNPQCLEDXXXXXXXXXXXXXXXXXXKDNKEIVVYKTSTDTDYDSFLADLPEDE 122
           M+SGVGVNPQCL                     DN E++V K+S+  DYD FLADLPE E
Sbjct: 1   MASGVGVNPQCLSAYQELKLGKKTKYIVFGLSPDNTEVIVLKSSSSQDYDEFLADLPETE 60

Query: 121 PRWAVYDFEFEKE-GAGKRNKLTFYSWVPDGAKIKAKMLFA 2
            RWAVYDFEFEKE GAGKRNKLTF+SW PD +KIK KMLFA
Sbjct: 61  CRWAVYDFEFEKEGGAGKRNKLTFFSWAPDDSKIKQKMLFA 101


>ref|XP_001875617.1| actin depolymerizing factor [Laccaria bicolor S238N-H82]
           gi|164648877|gb|EDR13119.1| actin depolymerizing factor
           [Laccaria bicolor S238N-H82]
          Length = 138

 Score =  122 bits (305), Expect = 1e-25
 Identities = 61/100 (61%), Positives = 67/100 (67%)
 Frame = -2

Query: 301 MSSGVGVNPQCLEDXXXXXXXXXXXXXXXXXXKDNKEIVVYKTSTDTDYDSFLADLPEDE 122
           MSSGVGVNP+C+E                     N EIVV KTS   DYD+F ADLPE  
Sbjct: 1   MSSGVGVNPECIEVFQALKLNKKHKYIIYKLNATNTEIVVDKTSDAQDYDTFTADLPETS 60

Query: 121 PRWAVYDFEFEKEGAGKRNKLTFYSWVPDGAKIKAKMLFA 2
           PRWAVYDF FEKEGAGKR+K+TFYSW PD AKIK KML+A
Sbjct: 61  PRWAVYDFAFEKEGAGKRHKITFYSWSPDDAKIKEKMLYA 100


>ref|XP_009546254.1| hypothetical protein HETIRDRAFT_383987 [Heterobasidion irregulare
           TC 32-1] gi|575066010|gb|ETW81624.1| hypothetical
           protein HETIRDRAFT_383987 [Heterobasidion irregulare TC
           32-1]
          Length = 138

 Score =  120 bits (300), Expect = 5e-25
 Identities = 59/100 (59%), Positives = 68/100 (68%)
 Frame = -2

Query: 301 MSSGVGVNPQCLEDXXXXXXXXXXXXXXXXXXKDNKEIVVYKTSTDTDYDSFLADLPEDE 122
           M+SGV VNP+CL                    KDN EI+V K+ST + YD F++DLPE E
Sbjct: 1   MASGVAVNPECLSAFQELKLGKKTKYIVYTVSKDNTEIIVEKSSTSSSYDEFISDLPEFE 60

Query: 121 PRWAVYDFEFEKEGAGKRNKLTFYSWVPDGAKIKAKMLFA 2
            RWAVYDFEFEKEGAGKRNK+ FYSW PD AK+K KMLFA
Sbjct: 61  CRWAVYDFEFEKEGAGKRNKICFYSWSPDDAKVKQKMLFA 100


>gb|KJA20159.1| hypothetical protein HYPSUDRAFT_68743 [Hypholoma sublateritium
           FD-334 SS-4]
          Length = 138

 Score =  118 bits (295), Expect = 2e-24
 Identities = 60/100 (60%), Positives = 67/100 (67%)
 Frame = -2

Query: 301 MSSGVGVNPQCLEDXXXXXXXXXXXXXXXXXXKDNKEIVVYKTSTDTDYDSFLADLPEDE 122
           M+SGVGVNP CL                    K   EIVV KTS+D DYD+FL+DLPE E
Sbjct: 1   MASGVGVNPDCLSAFQALKLKKEHKYIIFNLNKGFSEIVVEKTSSDGDYDTFLSDLPETE 60

Query: 121 PRWAVYDFEFEKEGAGKRNKLTFYSWVPDGAKIKAKMLFA 2
            RWA+YDFEFEKEGAGKRNK+ F SW PD AKIK KM+FA
Sbjct: 61  CRWAIYDFEFEKEGAGKRNKIIFISWAPDDAKIKQKMVFA 100


>gb|KIY46739.1| cofilin [Fistulina hepatica ATCC 64428]
          Length = 138

 Score =  117 bits (293), Expect = 3e-24
 Identities = 60/100 (60%), Positives = 65/100 (65%)
 Frame = -2

Query: 301 MSSGVGVNPQCLEDXXXXXXXXXXXXXXXXXXKDNKEIVVYKTSTDTDYDSFLADLPEDE 122
           MSSGVGVN  CL                     DN EIVV KTST TDYD FLADLPE +
Sbjct: 1   MSSGVGVNHDCLAKFQELKLGKKTKYIIFNLNDDNTEIVVEKTSTSTDYDEFLADLPEAD 60

Query: 121 PRWAVYDFEFEKEGAGKRNKLTFYSWVPDGAKIKAKMLFA 2
            RWAVYDFE+EKE AGKRNK+ F +W PD AKIK KM+FA
Sbjct: 61  CRWAVYDFEYEKEDAGKRNKICFIAWSPDDAKIKKKMVFA 100


>gb|KIO25853.1| hypothetical protein M407DRAFT_243931 [Tulasnella calospora MUT
           4182]
          Length = 139

 Score =  116 bits (291), Expect = 5e-24
 Identities = 58/99 (58%), Positives = 65/99 (65%)
 Frame = -2

Query: 298 SSGVGVNPQCLEDXXXXXXXXXXXXXXXXXXKDNKEIVVYKTSTDTDYDSFLADLPEDEP 119
           +SGVGVN +CL                        EIVV KTS + DY++FL DLPED P
Sbjct: 3   TSGVGVNQECLSTYQELKLKKAHKYIIFKLNDTLTEIVVDKTSKEADYEAFLGDLPEDAP 62

Query: 118 RWAVYDFEFEKEGAGKRNKLTFYSWVPDGAKIKAKMLFA 2
           RWAVYDFEFEK GAGKRNKLTFYSW PD AKIK KM++A
Sbjct: 63  RWAVYDFEFEKAGAGKRNKLTFYSWNPDNAKIKQKMVYA 101


>ref|XP_012178127.1| predicted protein [Fibroporia radiculosa]
           gi|403412144|emb|CCL98844.1| predicted protein
           [Fibroporia radiculosa]
          Length = 166

 Score =  115 bits (289), Expect = 9e-24
 Identities = 56/98 (57%), Positives = 64/98 (65%)
 Frame = -2

Query: 295 SGVGVNPQCLEDXXXXXXXXXXXXXXXXXXKDNKEIVVYKTSTDTDYDSFLADLPEDEPR 116
           SGVGV+P CL                      N EI+V K S   DYD FL++LPE EPR
Sbjct: 31  SGVGVSPDCLSAYQNLKLGKKIKYIVFTLNSTNTEIIVEKESQSNDYDEFLSNLPETEPR 90

Query: 115 WAVYDFEFEKEGAGKRNKLTFYSWVPDGAKIKAKMLFA 2
           WAVYDFE+EKEGAGKRNK+TF+SW PD +KIK KMLFA
Sbjct: 91  WAVYDFEYEKEGAGKRNKITFFSWSPDDSKIKQKMLFA 128


>ref|XP_007380427.1| hypothetical protein PUNSTDRAFT_111233 [Punctularia strigosozonata
           HHB-11173 SS5] gi|390603468|gb|EIN12860.1| hypothetical
           protein PUNSTDRAFT_111233 [Punctularia strigosozonata
           HHB-11173 SS5]
          Length = 138

 Score =  115 bits (289), Expect = 9e-24
 Identities = 57/100 (57%), Positives = 66/100 (66%)
 Frame = -2

Query: 301 MSSGVGVNPQCLEDXXXXXXXXXXXXXXXXXXKDNKEIVVYKTSTDTDYDSFLADLPEDE 122
           M+SGVGVNP CL                     DN EI+V KTS   DYD FLADLPE E
Sbjct: 1   MASGVGVNPDCLSAFQQLKLGKKIKYLIFSLSPDNTEIIVSKTSDSKDYDDFLADLPETE 60

Query: 121 PRWAVYDFEFEKEGAGKRNKLTFYSWVPDGAKIKAKMLFA 2
            R+AVYDFE+EKEGAGKRNK+ F++W PD +KIK KML+A
Sbjct: 61  CRYAVYDFEYEKEGAGKRNKICFFTWSPDDSKIKQKMLYA 100


>ref|XP_007363778.1| actin depolymerizing factor [Dichomitus squalens LYAD-421 SS1]
           gi|395330844|gb|EJF63226.1| actin depolymerizing factor
           [Dichomitus squalens LYAD-421 SS1]
          Length = 139

 Score =  115 bits (288), Expect = 1e-23
 Identities = 59/101 (58%), Positives = 66/101 (65%), Gaps = 1/101 (0%)
 Frame = -2

Query: 301 MSSGVGVNPQCLEDXXXXXXXXXXXXXXXXXXKDNKEIVVYKTS-TDTDYDSFLADLPED 125
           MSSGVGVNP+CL                    KDN EIVV KT  T   YD F+ DLPE+
Sbjct: 1   MSSGVGVNPECLSAYQELKLGKKSKYIIFTLSKDNTEIVVEKTGPTSATYDDFVGDLPEN 60

Query: 124 EPRWAVYDFEFEKEGAGKRNKLTFYSWVPDGAKIKAKMLFA 2
           EPRWAVYDF++EKE  GKR K+TF+SW PD AKIK KMLFA
Sbjct: 61  EPRWAVYDFDYEKEDGGKRTKITFFSWSPDDAKIKQKMLFA 101


>ref|XP_007870386.1| actin depolymerizing factor, partial [Gloeophyllum trabeum ATCC
           11539] gi|521720628|gb|EPQ51060.1| actin depolymerizing
           factor, partial [Gloeophyllum trabeum ATCC 11539]
          Length = 130

 Score =  114 bits (286), Expect = 2e-23
 Identities = 57/99 (57%), Positives = 67/99 (67%)
 Frame = -2

Query: 298 SSGVGVNPQCLEDXXXXXXXXXXXXXXXXXXKDNKEIVVYKTSTDTDYDSFLADLPEDEP 119
           +SGVGVNP+CLE                   KDN EI+V K S  ++Y+ FLADLPE+E 
Sbjct: 2   ASGVGVNPECLEAYQELKLRKTMKYIIFNLNKDNTEIIVEKKSDSSNYEEFLADLPENEC 61

Query: 118 RWAVYDFEFEKEGAGKRNKLTFYSWVPDGAKIKAKMLFA 2
           RWAVYDFE+EKEG GKRNKL F +W PD +KIK KMLFA
Sbjct: 62  RWAVYDFEYEKEGGGKRNKLCFIAWSPDDSKIKQKMLFA 100


>gb|KIM41051.1| hypothetical protein M413DRAFT_445794 [Hebeloma cylindrosporum h7]
          Length = 138

 Score =  113 bits (283), Expect = 4e-23
 Identities = 59/100 (59%), Positives = 66/100 (66%)
 Frame = -2

Query: 301 MSSGVGVNPQCLEDXXXXXXXXXXXXXXXXXXKDNKEIVVYKTSTDTDYDSFLADLPEDE 122
           M+SGVGVNPQC++                   KD  EIVV KTS   DYD F+A LPE E
Sbjct: 1   MASGVGVNPQCVDAFQDLKLKKKYKYIIFTLNKDFTEIVVDKTSDVQDYDEFVAALPETE 60

Query: 121 PRWAVYDFEFEKEGAGKRNKLTFYSWVPDGAKIKAKMLFA 2
            RWA+YDFEFEKEGAGKRNK+ F SW PD AKIK KM+FA
Sbjct: 61  CRWAIYDFEFEKEGAGKRNKIIFLSWSPDDAKIKQKMVFA 100


>ref|XP_007319810.1| hypothetical protein SERLADRAFT_392869 [Serpula lacrymans var.
           lacrymans S7.9] gi|336370128|gb|EGN98469.1| hypothetical
           protein SERLA73DRAFT_138937 [Serpula lacrymans var.
           lacrymans S7.3] gi|336382898|gb|EGO24048.1| hypothetical
           protein SERLADRAFT_392869 [Serpula lacrymans var.
           lacrymans S7.9]
          Length = 136

 Score =  113 bits (283), Expect = 4e-23
 Identities = 57/100 (57%), Positives = 65/100 (65%)
 Frame = -2

Query: 301 MSSGVGVNPQCLEDXXXXXXXXXXXXXXXXXXKDNKEIVVYKTSTDTDYDSFLADLPEDE 122
           M+SGVGVNP+CL                     DN EI+V+KTS   DYD FLA+LPE E
Sbjct: 1   MASGVGVNPECLSAFETLKLGKKLKFIVFTLNSDNTEIIVHKTSDSPDYDEFLAELPERE 60

Query: 121 PRWAVYDFEFEKEGAGKRNKLTFYSWVPDGAKIKAKMLFA 2
            RWAVYDFE+EK G GKRNK+ FYSW PD AK+K KML A
Sbjct: 61  CRWAVYDFEYEK-GEGKRNKICFYSWSPDDAKVKQKMLLA 99


>ref|XP_002477528.1| predicted protein [Postia placenta Mad-698-R]
           gi|220722798|gb|EED77274.1| predicted protein, partial
           [Postia placenta Mad-698-R]
          Length = 169

 Score =  113 bits (282), Expect = 6e-23
 Identities = 58/98 (59%), Positives = 64/98 (65%)
 Frame = -2

Query: 295 SGVGVNPQCLEDXXXXXXXXXXXXXXXXXXKDNKEIVVYKTSTDTDYDSFLADLPEDEPR 116
           SGV V+P CL                     D+ EI+V K S  T+YD FL DLPE EPR
Sbjct: 4   SGVAVSPACLT-------AYQNLKLGKKINPDHTEIIVEKESQSTNYDDFLGDLPEVEPR 56

Query: 115 WAVYDFEFEKEGAGKRNKLTFYSWVPDGAKIKAKMLFA 2
           WAVYDFEFEKEGAGKRNK+TFYSW PD +KIK KMLFA
Sbjct: 57  WAVYDFEFEKEGAGKRNKITFYSWSPDDSKIKQKMLFA 94


>gb|KIY73841.1| actin depolymerizing factor [Cylindrobasidium torrendii FP15055
           ss-10]
          Length = 138

 Score =  112 bits (281), Expect = 7e-23
 Identities = 55/100 (55%), Positives = 64/100 (64%)
 Frame = -2

Query: 301 MSSGVGVNPQCLEDXXXXXXXXXXXXXXXXXXKDNKEIVVYKTSTDTDYDSFLADLPEDE 122
           M+SG+ V+P+CLE+                   +  EIVV KTS    YD F+ADLPE E
Sbjct: 1   MASGIAVSPKCLEEFQTLKLGKKTKYIIYRINNEKTEIVVEKTSDSASYDEFIADLPETE 60

Query: 121 PRWAVYDFEFEKEGAGKRNKLTFYSWVPDGAKIKAKMLFA 2
            RWAVYDFEFEKEG GKRNK+ F+ W PD AKIK KMLFA
Sbjct: 61  CRWAVYDFEFEKEGEGKRNKICFFMWSPDDAKIKDKMLFA 100


>gb|KDR83149.1| hypothetical protein GALMADRAFT_238995 [Galerina marginata CBS
           339.88]
          Length = 138

 Score =  112 bits (281), Expect = 7e-23
 Identities = 55/100 (55%), Positives = 66/100 (66%)
 Frame = -2

Query: 301 MSSGVGVNPQCLEDXXXXXXXXXXXXXXXXXXKDNKEIVVYKTSTDTDYDSFLADLPEDE 122
           M+SGVGVN  C+                     +  E++V KTS++ DYD+FLADLPE E
Sbjct: 1   MASGVGVNELCISTFQELKLKKKYKYIIFTLNSNFTEVIVEKTSSEQDYDAFLADLPETE 60

Query: 121 PRWAVYDFEFEKEGAGKRNKLTFYSWVPDGAKIKAKMLFA 2
            RWA+YDFEFEKEGAGKRNK+ F SW PD AKIK KM+FA
Sbjct: 61  CRWAIYDFEFEKEGAGKRNKIIFLSWSPDDAKIKQKMVFA 100


>ref|XP_007348155.1| actin depolymerizing factor [Auricularia delicata TFB-10046 SS5]
           gi|393236250|gb|EJD43800.1| actin depolymerizing factor
           [Auricularia delicata TFB-10046 SS5]
          Length = 138

 Score =  112 bits (280), Expect = 9e-23
 Identities = 58/100 (58%), Positives = 64/100 (64%)
 Frame = -2

Query: 301 MSSGVGVNPQCLEDXXXXXXXXXXXXXXXXXXKDNKEIVVYKTSTDTDYDSFLADLPEDE 122
           MSSGV VNP+CLE                   K N EIVV KTS    YD F+  LPE E
Sbjct: 1   MSSGVAVNPECLERFQELKLGKKLKYIIFSLNKTNTEIVVEKTSEGGSYDDFIGQLPEAE 60

Query: 121 PRWAVYDFEFEKEGAGKRNKLTFYSWVPDGAKIKAKMLFA 2
            RWAVYDFE+EKEGAGKRNKL F+SW PD +KIK KM+FA
Sbjct: 61  CRWAVYDFEYEKEGAGKRNKLCFFSWSPDDSKIKQKMVFA 100


>ref|XP_007845436.1| actin depolymerizing factor [Moniliophthora roreri MCA 2997]
           gi|554914059|gb|ESK95225.1| actin depolymerizing factor
           [Moniliophthora roreri MCA 2997]
          Length = 138

 Score =  112 bits (279), Expect = 1e-22
 Identities = 55/100 (55%), Positives = 65/100 (65%)
 Frame = -2

Query: 301 MSSGVGVNPQCLEDXXXXXXXXXXXXXXXXXXKDNKEIVVYKTSTDTDYDSFLADLPEDE 122
           M+SGV VNP CL++                      EI+V K S  ++YD FLADLPE +
Sbjct: 1   MASGVQVNPACLQEYQTLKLGKKIKYIIFNLNDAKTEIIVEKKSESSNYDDFLADLPEGD 60

Query: 121 PRWAVYDFEFEKEGAGKRNKLTFYSWVPDGAKIKAKMLFA 2
            RWAVYDFEF+KEGAGKRNKL F+SW PD AK+K KMLFA
Sbjct: 61  CRWAVYDFEFDKEGAGKRNKLCFFSWSPDDAKVKQKMLFA 100


>ref|XP_007304898.1| hypothetical protein STEHIDRAFT_122014 [Stereum hirsutum FP-91666
           SS1] gi|389744823|gb|EIM86005.1| hypothetical protein
           STEHIDRAFT_122014 [Stereum hirsutum FP-91666 SS1]
          Length = 138

 Score =  112 bits (279), Expect = 1e-22
 Identities = 58/100 (58%), Positives = 64/100 (64%)
 Frame = -2

Query: 301 MSSGVGVNPQCLEDXXXXXXXXXXXXXXXXXXKDNKEIVVYKTSTDTDYDSFLADLPEDE 122
           M+SGV VNP CLE                   KD  EIVV K ST T YD F+ADLPE E
Sbjct: 1   MASGVAVNPACLEAFQELKLGKKTKYLIFAISKDLTEIVVEKKSTSTSYDEFVADLPEAE 60

Query: 121 PRWAVYDFEFEKEGAGKRNKLTFYSWVPDGAKIKAKMLFA 2
            RWA+YDFEFEKEGAG RNK+ F SW PD +K+K KMLFA
Sbjct: 61  CRWAIYDFEFEKEGAGIRNKICFISWSPDDSKVKQKMLFA 100