BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Akebia25_contig00052239
         (311 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003632628.1| PREDICTED: LOW QUALITY PROTEIN: DNA replicat...   184   1e-44
emb|CBI32613.3| unnamed protein product [Vitis vinifera]              184   1e-44
ref|XP_004156637.1| PREDICTED: DNA helicase MCM8-like [Cucumis s...   182   4e-44
ref|XP_004137962.1| PREDICTED: DNA helicase MCM8-like [Cucumis s...   182   4e-44
ref|XP_007027246.1| Minichromosome maintenance 8 [Theobroma caca...   182   5e-44
ref|XP_002525183.1| DNA replication licensing factor MCM8, putat...   174   1e-41
gb|EYU41631.1| hypothetical protein MIMGU_mgv1a026728mg, partial...   173   2e-41
ref|XP_006361775.1| PREDICTED: DNA helicase MCM8-like [Solanum t...   173   2e-41
ref|XP_004246695.1| PREDICTED: DNA helicase MCM8-like [Solanum l...   172   4e-41
ref|XP_004493943.1| PREDICTED: DNA helicase MCM8-like [Cicer ari...   171   7e-41
ref|XP_006843219.1| hypothetical protein AMTR_s00080p00032690 [A...   171   1e-40
ref|XP_006604552.1| PREDICTED: DNA helicase MCM8-like isoform X2...   170   2e-40
ref|XP_006604551.1| PREDICTED: DNA helicase MCM8-like isoform X1...   170   2e-40
gb|EXC16277.1| DNA replication licensing factor MCM8 [Morus nota...   169   4e-40
ref|XP_007162721.1| hypothetical protein PHAVU_001G174700g [Phas...   169   5e-40
ref|XP_006381495.1| minichromosome maintenance family protein [P...   168   8e-40
ref|XP_006480688.1| PREDICTED: DNA helicase MCM8-like [Citrus si...   166   2e-39
ref|XP_006428877.1| hypothetical protein CICLE_v10013573mg [Citr...   165   7e-39
ref|XP_007208753.1| hypothetical protein PRUPE_ppa016452mg, part...   164   9e-39
ref|XP_004306229.1| PREDICTED: DNA helicase MCM8-like [Fragaria ...   163   3e-38

>ref|XP_003632628.1| PREDICTED: LOW QUALITY PROTEIN: DNA replication licensing factor
           MCM8-like [Vitis vinifera]
          Length = 754

 Score =  184 bits (467), Expect = 1e-44
 Identities = 89/103 (86%), Positives = 98/103 (95%)
 Frame = -2

Query: 310 VPRTVECELTEDLVDACIPGDVVTVTGIIKVINNYIDVGGGKSKNKNQGLYYLYLEAVSI 131
           VPRTVECELTEDLVDACIPGDVVTVTGII+ INNY+D+GGGKSKNKNQGL+YLYLEAVSI
Sbjct: 234 VPRTVECELTEDLVDACIPGDVVTVTGIIRQINNYMDIGGGKSKNKNQGLFYLYLEAVSI 293

Query: 130 KNTKSQSLPEDLQDTSADVRATELFDLYSFSPRDLEFIVKFSE 2
           KN+KSQ + EDLQD++AD RATEL DL+SFSPRDLEFIVKFSE
Sbjct: 294 KNSKSQYISEDLQDSNADARATELLDLFSFSPRDLEFIVKFSE 336


>emb|CBI32613.3| unnamed protein product [Vitis vinifera]
          Length = 553

 Score =  184 bits (467), Expect = 1e-44
 Identities = 89/103 (86%), Positives = 98/103 (95%)
 Frame = -2

Query: 310 VPRTVECELTEDLVDACIPGDVVTVTGIIKVINNYIDVGGGKSKNKNQGLYYLYLEAVSI 131
           VPRTVECELTEDLVDACIPGDVVTVTGII+ INNY+D+GGGKSKNKNQGL+YLYLEAVSI
Sbjct: 11  VPRTVECELTEDLVDACIPGDVVTVTGIIRQINNYMDIGGGKSKNKNQGLFYLYLEAVSI 70

Query: 130 KNTKSQSLPEDLQDTSADVRATELFDLYSFSPRDLEFIVKFSE 2
           KN+KSQ + EDLQD++AD RATEL DL+SFSPRDLEFIVKFSE
Sbjct: 71  KNSKSQYISEDLQDSNADARATELLDLFSFSPRDLEFIVKFSE 113


>ref|XP_004156637.1| PREDICTED: DNA helicase MCM8-like [Cucumis sativus]
          Length = 786

 Score =  182 bits (462), Expect = 4e-44
 Identities = 87/102 (85%), Positives = 98/102 (96%)
 Frame = -2

Query: 310 VPRTVECELTEDLVDACIPGDVVTVTGIIKVINNYIDVGGGKSKNKNQGLYYLYLEAVSI 131
           VPRTVECELTEDLVDACIPGDVVTVTGII+VINNY+D+GGGKSK+KNQG YYLYLEAVSI
Sbjct: 221 VPRTVECELTEDLVDACIPGDVVTVTGIIRVINNYMDIGGGKSKSKNQGFYYLYLEAVSI 280

Query: 130 KNTKSQSLPEDLQDTSADVRATELFDLYSFSPRDLEFIVKFS 5
           KN+KSQS PE+LQD++++ RATEL DL+SFSPRDLEFIVKFS
Sbjct: 281 KNSKSQSTPEELQDSNSNARATELLDLFSFSPRDLEFIVKFS 322


>ref|XP_004137962.1| PREDICTED: DNA helicase MCM8-like [Cucumis sativus]
          Length = 793

 Score =  182 bits (462), Expect = 4e-44
 Identities = 87/102 (85%), Positives = 98/102 (96%)
 Frame = -2

Query: 310 VPRTVECELTEDLVDACIPGDVVTVTGIIKVINNYIDVGGGKSKNKNQGLYYLYLEAVSI 131
           VPRTVECELTEDLVDACIPGDVVTVTGII+VINNY+D+GGGKSK+KNQG YYLYLEAVSI
Sbjct: 228 VPRTVECELTEDLVDACIPGDVVTVTGIIRVINNYMDIGGGKSKSKNQGFYYLYLEAVSI 287

Query: 130 KNTKSQSLPEDLQDTSADVRATELFDLYSFSPRDLEFIVKFS 5
           KN+KSQS PE+LQD++++ RATEL DL+SFSPRDLEFIVKFS
Sbjct: 288 KNSKSQSTPEELQDSNSNARATELLDLFSFSPRDLEFIVKFS 329


>ref|XP_007027246.1| Minichromosome maintenance 8 [Theobroma cacao]
           gi|508715851|gb|EOY07748.1| Minichromosome maintenance 8
           [Theobroma cacao]
          Length = 855

 Score =  182 bits (461), Expect = 5e-44
 Identities = 86/103 (83%), Positives = 98/103 (95%)
 Frame = -2

Query: 310 VPRTVECELTEDLVDACIPGDVVTVTGIIKVINNYIDVGGGKSKNKNQGLYYLYLEAVSI 131
           VPRTVECELTEDLVD CIPGDVVTVTGII+VINNY+D+GGGKSK++NQG YYLY+EAVSI
Sbjct: 299 VPRTVECELTEDLVDLCIPGDVVTVTGIIRVINNYMDIGGGKSKSRNQGFYYLYIEAVSI 358

Query: 130 KNTKSQSLPEDLQDTSADVRATELFDLYSFSPRDLEFIVKFSE 2
           KN+KSQS+PEDLQD+ ++ RATEL DL+SFSPRDLEFIVKFSE
Sbjct: 359 KNSKSQSVPEDLQDSDSNARATELVDLFSFSPRDLEFIVKFSE 401


>ref|XP_002525183.1| DNA replication licensing factor MCM8, putative [Ricinus communis]
           gi|223535480|gb|EEF37149.1| DNA replication licensing
           factor MCM8, putative [Ricinus communis]
          Length = 757

 Score =  174 bits (440), Expect = 1e-41
 Identities = 82/103 (79%), Positives = 92/103 (89%)
 Frame = -2

Query: 310 VPRTVECELTEDLVDACIPGDVVTVTGIIKVINNYIDVGGGKSKNKNQGLYYLYLEAVSI 131
           VPRTVECELTEDLVDACIPGDVVTVTGII+ INNY+D+GGGKSK KNQG YYLYLE +SI
Sbjct: 241 VPRTVECELTEDLVDACIPGDVVTVTGIIRTINNYVDIGGGKSKGKNQGFYYLYLEVISI 300

Query: 130 KNTKSQSLPEDLQDTSADVRATELFDLYSFSPRDLEFIVKFSE 2
           KN+KSQS   D+QD  ++ RATEL DL+SFSP+DLEFIVKFSE
Sbjct: 301 KNSKSQSTSSDMQDARSNARATELSDLFSFSPKDLEFIVKFSE 343


>gb|EYU41631.1| hypothetical protein MIMGU_mgv1a026728mg, partial [Mimulus
           guttatus]
          Length = 717

 Score =  173 bits (439), Expect = 2e-41
 Identities = 83/103 (80%), Positives = 93/103 (90%)
 Frame = -2

Query: 310 VPRTVECELTEDLVDACIPGDVVTVTGIIKVINNYIDVGGGKSKNKNQGLYYLYLEAVSI 131
           VPRTV+CE+TEDLVDACIPGDVVTVTGII+VINNY+D+GGGKSK KNQ LYYLYL+ VSI
Sbjct: 193 VPRTVDCEMTEDLVDACIPGDVVTVTGIIRVINNYMDIGGGKSKGKNQSLYYLYLDVVSI 252

Query: 130 KNTKSQSLPEDLQDTSADVRATELFDLYSFSPRDLEFIVKFSE 2
            N+KSQS PED+Q T ++ RATE  DLYSFSPRDLEFIVKFSE
Sbjct: 253 INSKSQSAPEDMQHTDSNARATEHLDLYSFSPRDLEFIVKFSE 295


>ref|XP_006361775.1| PREDICTED: DNA helicase MCM8-like [Solanum tuberosum]
          Length = 791

 Score =  173 bits (439), Expect = 2e-41
 Identities = 85/103 (82%), Positives = 94/103 (91%)
 Frame = -2

Query: 310 VPRTVECELTEDLVDACIPGDVVTVTGIIKVINNYIDVGGGKSKNKNQGLYYLYLEAVSI 131
           VPRTVECELTEDLVDACIPGD+VTVTGII+VINNY+D+GGGKSK KNQGLYYLYLE VSI
Sbjct: 238 VPRTVECELTEDLVDACIPGDIVTVTGIIRVINNYMDIGGGKSKGKNQGLYYLYLEVVSI 297

Query: 130 KNTKSQSLPEDLQDTSADVRATELFDLYSFSPRDLEFIVKFSE 2
            N+KSQS PE  QD + + +ATELFDLYSFSP+DLEFIVKFSE
Sbjct: 298 TNSKSQSTPEGSQD-NPNAKATELFDLYSFSPKDLEFIVKFSE 339


>ref|XP_004246695.1| PREDICTED: DNA helicase MCM8-like [Solanum lycopersicum]
          Length = 758

 Score =  172 bits (436), Expect = 4e-41
 Identities = 84/103 (81%), Positives = 94/103 (91%)
 Frame = -2

Query: 310 VPRTVECELTEDLVDACIPGDVVTVTGIIKVINNYIDVGGGKSKNKNQGLYYLYLEAVSI 131
           VPRTVECELTEDLVDACIPGD+VTVTGII+VINNY+D+GGGKSK KNQGLYYLYLE VSI
Sbjct: 238 VPRTVECELTEDLVDACIPGDIVTVTGIIRVINNYMDIGGGKSKGKNQGLYYLYLEVVSI 297

Query: 130 KNTKSQSLPEDLQDTSADVRATELFDLYSFSPRDLEFIVKFSE 2
            N+KSQS+PE  QD + + RAT+L DLYSFSP+DLEFIVKFSE
Sbjct: 298 TNSKSQSMPEGSQD-NPNARATDLLDLYSFSPKDLEFIVKFSE 339


>ref|XP_004493943.1| PREDICTED: DNA helicase MCM8-like [Cicer arietinum]
          Length = 803

 Score =  171 bits (434), Expect = 7e-41
 Identities = 81/103 (78%), Positives = 95/103 (92%)
 Frame = -2

Query: 310 VPRTVECELTEDLVDACIPGDVVTVTGIIKVINNYIDVGGGKSKNKNQGLYYLYLEAVSI 131
           VPRTVECELT+DLVDACIPGDVVTVTGII+ INNY+D+GGGKSKN+NQG YYLYLEAVSI
Sbjct: 240 VPRTVECELTQDLVDACIPGDVVTVTGIIRGINNYMDIGGGKSKNRNQGFYYLYLEAVSI 299

Query: 130 KNTKSQSLPEDLQDTSADVRATELFDLYSFSPRDLEFIVKFSE 2
            N+KSQS+PED QD++A  R TELF+L+SFS +DLEF+VKF+E
Sbjct: 300 INSKSQSIPEDSQDSNAKARPTELFNLFSFSSKDLEFVVKFAE 342


>ref|XP_006843219.1| hypothetical protein AMTR_s00080p00032690 [Amborella trichopoda]
           gi|548845503|gb|ERN04894.1| hypothetical protein
           AMTR_s00080p00032690 [Amborella trichopoda]
          Length = 792

 Score =  171 bits (432), Expect = 1e-40
 Identities = 85/103 (82%), Positives = 93/103 (90%)
 Frame = -2

Query: 310 VPRTVECELTEDLVDACIPGDVVTVTGIIKVINNYIDVGGGKSKNKNQGLYYLYLEAVSI 131
           +PRTVECELTEDLVDACIPGDVVTV GIIKVIN Y+DVGGGKSK+KNQGLYYLYLEAVSI
Sbjct: 236 MPRTVECELTEDLVDACIPGDVVTVCGIIKVINTYMDVGGGKSKSKNQGLYYLYLEAVSI 295

Query: 130 KNTKSQSLPEDLQDTSADVRATELFDLYSFSPRDLEFIVKFSE 2
           +N+KSQS  EDLQ T+ D RA  L DLY+FSPRDLEFIV+FSE
Sbjct: 296 RNSKSQSAAEDLQVTNVDFRAGLLTDLYAFSPRDLEFIVRFSE 338


>ref|XP_006604552.1| PREDICTED: DNA helicase MCM8-like isoform X2 [Glycine max]
          Length = 665

 Score =  170 bits (431), Expect = 2e-40
 Identities = 80/103 (77%), Positives = 93/103 (90%)
 Frame = -2

Query: 310 VPRTVECELTEDLVDACIPGDVVTVTGIIKVINNYIDVGGGKSKNKNQGLYYLYLEAVSI 131
           VPRTVECELT+DLVDACIPGDVVTVTGII+ IN Y+D+GGGKSKNKNQG YYLYLEAVSI
Sbjct: 101 VPRTVECELTQDLVDACIPGDVVTVTGIIRGINTYMDIGGGKSKNKNQGFYYLYLEAVSI 160

Query: 130 KNTKSQSLPEDLQDTSADVRATELFDLYSFSPRDLEFIVKFSE 2
           KN+KSQS+PEDL D++   R TELFDL+SFS +DLEF+ KF++
Sbjct: 161 KNSKSQSVPEDLPDSNPKARPTELFDLFSFSSKDLEFVAKFAQ 203


>ref|XP_006604551.1| PREDICTED: DNA helicase MCM8-like isoform X1 [Glycine max]
          Length = 787

 Score =  170 bits (431), Expect = 2e-40
 Identities = 80/103 (77%), Positives = 93/103 (90%)
 Frame = -2

Query: 310 VPRTVECELTEDLVDACIPGDVVTVTGIIKVINNYIDVGGGKSKNKNQGLYYLYLEAVSI 131
           VPRTVECELT+DLVDACIPGDVVTVTGII+ IN Y+D+GGGKSKNKNQG YYLYLEAVSI
Sbjct: 223 VPRTVECELTQDLVDACIPGDVVTVTGIIRGINTYMDIGGGKSKNKNQGFYYLYLEAVSI 282

Query: 130 KNTKSQSLPEDLQDTSADVRATELFDLYSFSPRDLEFIVKFSE 2
           KN+KSQS+PEDL D++   R TELFDL+SFS +DLEF+ KF++
Sbjct: 283 KNSKSQSVPEDLPDSNPKARPTELFDLFSFSSKDLEFVAKFAQ 325


>gb|EXC16277.1| DNA replication licensing factor MCM8 [Morus notabilis]
          Length = 818

 Score =  169 bits (428), Expect = 4e-40
 Identities = 81/103 (78%), Positives = 92/103 (89%)
 Frame = -2

Query: 310 VPRTVECELTEDLVDACIPGDVVTVTGIIKVINNYIDVGGGKSKNKNQGLYYLYLEAVSI 131
           VPRTVECEL+EDLVDACIPGDVVTVTGII+VINNY+D+GGGKSK+KNQG YYLYLEA SI
Sbjct: 238 VPRTVECELSEDLVDACIPGDVVTVTGIIRVINNYMDIGGGKSKSKNQGFYYLYLEAFSI 297

Query: 130 KNTKSQSLPEDLQDTSADVRATELFDLYSFSPRDLEFIVKFSE 2
           KN+KS S  ED +D++   R TELFDL+SFS RDLEF+VKFSE
Sbjct: 298 KNSKSNSSSEDSEDSNCKARPTELFDLFSFSSRDLEFVVKFSE 340


>ref|XP_007162721.1| hypothetical protein PHAVU_001G174700g [Phaseolus vulgaris]
           gi|561036185|gb|ESW34715.1| hypothetical protein
           PHAVU_001G174700g [Phaseolus vulgaris]
          Length = 805

 Score =  169 bits (427), Expect = 5e-40
 Identities = 79/103 (76%), Positives = 94/103 (91%)
 Frame = -2

Query: 310 VPRTVECELTEDLVDACIPGDVVTVTGIIKVINNYIDVGGGKSKNKNQGLYYLYLEAVSI 131
           VPRTVECELT+DLVDACIPGDVVTVTGII+ IN+Y+D+GGGKSKN+NQG YYLYLEAVSI
Sbjct: 240 VPRTVECELTQDLVDACIPGDVVTVTGIIRGINSYMDIGGGKSKNRNQGFYYLYLEAVSI 299

Query: 130 KNTKSQSLPEDLQDTSADVRATELFDLYSFSPRDLEFIVKFSE 2
           KN+KSQS+ EDLQD++   R  ELFDL+SFS +DLEF+VKF++
Sbjct: 300 KNSKSQSMSEDLQDSNPKTRPAELFDLFSFSSKDLEFVVKFAQ 342


>ref|XP_006381495.1| minichromosome maintenance family protein [Populus trichocarpa]
           gi|550336199|gb|ERP59292.1| minichromosome maintenance
           family protein [Populus trichocarpa]
          Length = 745

 Score =  168 bits (425), Expect = 8e-40
 Identities = 82/103 (79%), Positives = 90/103 (87%)
 Frame = -2

Query: 310 VPRTVECELTEDLVDACIPGDVVTVTGIIKVINNYIDVGGGKSKNKNQGLYYLYLEAVSI 131
           VPRTVECELTEDLVDACIPGDVVTVTGIIK  N+ +D GGGKSKNKNQG YYLYLE VSI
Sbjct: 234 VPRTVECELTEDLVDACIPGDVVTVTGIIKTFNSNLDTGGGKSKNKNQGFYYLYLEVVSI 293

Query: 130 KNTKSQSLPEDLQDTSADVRATELFDLYSFSPRDLEFIVKFSE 2
           KN+K QS  ++LQD+  + RATEL DL+SFSPRDLEFIVKFSE
Sbjct: 294 KNSKLQSTSDNLQDSKCNARATELSDLFSFSPRDLEFIVKFSE 336


>ref|XP_006480688.1| PREDICTED: DNA helicase MCM8-like [Citrus sinensis]
          Length = 754

 Score =  166 bits (421), Expect = 2e-39
 Identities = 80/103 (77%), Positives = 95/103 (92%)
 Frame = -2

Query: 310 VPRTVECELTEDLVDACIPGDVVTVTGIIKVINNYIDVGGGKSKNKNQGLYYLYLEAVSI 131
           VPRTVECEL+EDLVDACIPGDVVTVTGII+VINNY+D+GGGKSK+K+QG YYL+LEAVS+
Sbjct: 234 VPRTVECELSEDLVDACIPGDVVTVTGIIRVINNYMDIGGGKSKSKSQGFYYLFLEAVSV 293

Query: 130 KNTKSQSLPEDLQDTSADVRATELFDLYSFSPRDLEFIVKFSE 2
           KN+KSQS  EDLQ ++ + RA+E  +L+SFSPRDLEFIVKFSE
Sbjct: 294 KNSKSQSDTEDLQGSNCNARASEQANLFSFSPRDLEFIVKFSE 336


>ref|XP_006428877.1| hypothetical protein CICLE_v10013573mg [Citrus clementina]
           gi|557530934|gb|ESR42117.1| hypothetical protein
           CICLE_v10013573mg [Citrus clementina]
          Length = 720

 Score =  165 bits (417), Expect = 7e-39
 Identities = 79/103 (76%), Positives = 94/103 (91%)
 Frame = -2

Query: 310 VPRTVECELTEDLVDACIPGDVVTVTGIIKVINNYIDVGGGKSKNKNQGLYYLYLEAVSI 131
           VPRTVECEL+EDLVD CIPGDVVTVTGII+VINNY+D+GGGKSK+K+QG YYL+LEAVS+
Sbjct: 234 VPRTVECELSEDLVDVCIPGDVVTVTGIIRVINNYMDIGGGKSKSKSQGFYYLFLEAVSV 293

Query: 130 KNTKSQSLPEDLQDTSADVRATELFDLYSFSPRDLEFIVKFSE 2
           KN+KSQS  EDLQ ++ + RA+E  +L+SFSPRDLEFIVKFSE
Sbjct: 294 KNSKSQSDTEDLQGSNCNARASEQANLFSFSPRDLEFIVKFSE 336


>ref|XP_007208753.1| hypothetical protein PRUPE_ppa016452mg, partial [Prunus persica]
           gi|462404395|gb|EMJ09952.1| hypothetical protein
           PRUPE_ppa016452mg, partial [Prunus persica]
          Length = 722

 Score =  164 bits (416), Expect = 9e-39
 Identities = 82/103 (79%), Positives = 93/103 (90%)
 Frame = -2

Query: 310 VPRTVECELTEDLVDACIPGDVVTVTGIIKVINNYIDVGGGKSKNKNQGLYYLYLEAVSI 131
           VPRTVECEL EDLVDACIPGDVVTVTGII+VINNY+D+GGGKSK K+QG+YYLYLEAVSI
Sbjct: 200 VPRTVECELLEDLVDACIPGDVVTVTGIIRVINNYMDIGGGKSKGKSQGIYYLYLEAVSI 259

Query: 130 KNTKSQSLPEDLQDTSADVRATELFDLYSFSPRDLEFIVKFSE 2
           KN+KSQS PED+QD++++  A  L D  SFSPRDLEFIVKFSE
Sbjct: 260 KNSKSQSTPEDVQDSNSNDMA--LVDFCSFSPRDLEFIVKFSE 300


>ref|XP_004306229.1| PREDICTED: DNA helicase MCM8-like [Fragaria vesca subsp. vesca]
          Length = 789

 Score =  163 bits (412), Expect = 3e-38
 Identities = 81/103 (78%), Positives = 92/103 (89%)
 Frame = -2

Query: 310 VPRTVECELTEDLVDACIPGDVVTVTGIIKVINNYIDVGGGKSKNKNQGLYYLYLEAVSI 131
           VPRTVECEL EDLVDACIPGDVVTVTGII+VINNY+D+GGGKSK K+QG+YYLYLEAVSI
Sbjct: 235 VPRTVECELLEDLVDACIPGDVVTVTGIIRVINNYMDIGGGKSKGKSQGIYYLYLEAVSI 294

Query: 130 KNTKSQSLPEDLQDTSADVRATELFDLYSFSPRDLEFIVKFSE 2
           KN+KSQS+PE  QD+S++  +  L D  SFSPRDLEFIVKFSE
Sbjct: 295 KNSKSQSMPEGAQDSSSNAGSLALVD--SFSPRDLEFIVKFSE 335