BLASTX nr result
ID: Akebia25_contig00027259
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Akebia25_contig00027259
(294 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002276157.2| PREDICTED: clathrin interactor EPSIN 1-like ... 153 2e-35
emb|CBI20607.3| unnamed protein product [Vitis vinifera] 153 2e-35
ref|XP_002276103.1| PREDICTED: clathrin interactor EPSIN 1-like ... 153 2e-35
ref|XP_007222298.1| hypothetical protein PRUPE_ppa003357mg [Prun... 145 8e-33
ref|XP_002516482.1| Clathrin interactor, putative [Ricinus commu... 144 2e-32
gb|EXB66907.1| hypothetical protein L484_019545 [Morus notabilis] 142 5e-32
ref|XP_006340249.1| PREDICTED: clathrin interactor EPSIN 1-like ... 139 3e-31
ref|XP_004251416.1| PREDICTED: clathrin interactor EPSIN 1-like ... 138 9e-31
ref|XP_006475977.1| PREDICTED: clathrin interactor EPSIN 1-like ... 135 6e-30
ref|XP_006450772.1| hypothetical protein CICLE_v10007888mg [Citr... 135 6e-30
ref|XP_006450769.1| hypothetical protein CICLE_v10007888mg [Citr... 135 6e-30
ref|XP_006450767.1| hypothetical protein CICLE_v10007888mg [Citr... 135 6e-30
gb|EYU20387.1| hypothetical protein MIMGU_mgv1a004114mg [Mimulus... 134 1e-29
ref|XP_006355310.1| PREDICTED: clathrin interactor EPSIN 1-like ... 134 1e-29
ref|XP_006355309.1| PREDICTED: clathrin interactor EPSIN 1-like ... 134 1e-29
ref|XP_003550292.1| PREDICTED: clathrin interactor EPSIN 1 isofo... 134 2e-29
ref|XP_003544513.1| PREDICTED: clathrin interactor EPSIN 1-like ... 134 2e-29
gb|EYU29308.1| hypothetical protein MIMGU_mgv1a004074mg [Mimulus... 132 4e-29
ref|XP_007012223.1| ENTH/VHS family protein [Theobroma cacao] gi... 132 4e-29
ref|XP_004245127.1| PREDICTED: clathrin interactor EPSIN 1-like ... 132 7e-29
>ref|XP_002276157.2| PREDICTED: clathrin interactor EPSIN 1-like isoform 2 [Vitis
vinifera]
Length = 552
Score = 153 bits (387), Expect = 2e-35
Identities = 72/98 (73%), Positives = 90/98 (91%)
Frame = +1
Query: 1 SLSGFEFVEPNGKDMGINVRKKAETIISLLNDKSKIQEVRNKAAANRDKYVGLSSTGITN 180
SLSGFE+VEPNGKD+GINVRKKAETI++LLN+K KIQEVRNKAAANRDK+ GLSS+G+T
Sbjct: 100 SLSGFEYVEPNGKDVGINVRKKAETIVALLNNKEKIQEVRNKAAANRDKFFGLSSSGVTY 159
Query: 181 KSTSASYGGSSFQHSDRYGGVSAKKDGEIFRDSYRDRD 294
KS+SA YG SSFQ +D++GG+S+K++ + FRDSY+DRD
Sbjct: 160 KSSSAPYGSSSFQSADQHGGMSSKRENDSFRDSYKDRD 197
>emb|CBI20607.3| unnamed protein product [Vitis vinifera]
Length = 608
Score = 153 bits (387), Expect = 2e-35
Identities = 72/98 (73%), Positives = 90/98 (91%)
Frame = +1
Query: 1 SLSGFEFVEPNGKDMGINVRKKAETIISLLNDKSKIQEVRNKAAANRDKYVGLSSTGITN 180
SLSGFE+VEPNGKD+GINVRKKAETI++LLN+K KIQEVRNKAAANRDK+ GLSS+G+T
Sbjct: 156 SLSGFEYVEPNGKDVGINVRKKAETIVALLNNKEKIQEVRNKAAANRDKFFGLSSSGVTY 215
Query: 181 KSTSASYGGSSFQHSDRYGGVSAKKDGEIFRDSYRDRD 294
KS+SA YG SSFQ +D++GG+S+K++ + FRDSY+DRD
Sbjct: 216 KSSSAPYGSSSFQSADQHGGMSSKRENDSFRDSYKDRD 253
>ref|XP_002276103.1| PREDICTED: clathrin interactor EPSIN 1-like isoform 1 [Vitis
vinifera]
Length = 565
Score = 153 bits (387), Expect = 2e-35
Identities = 72/98 (73%), Positives = 90/98 (91%)
Frame = +1
Query: 1 SLSGFEFVEPNGKDMGINVRKKAETIISLLNDKSKIQEVRNKAAANRDKYVGLSSTGITN 180
SLSGFE+VEPNGKD+GINVRKKAETI++LLN+K KIQEVRNKAAANRDK+ GLSS+G+T
Sbjct: 113 SLSGFEYVEPNGKDVGINVRKKAETIVALLNNKEKIQEVRNKAAANRDKFFGLSSSGVTY 172
Query: 181 KSTSASYGGSSFQHSDRYGGVSAKKDGEIFRDSYRDRD 294
KS+SA YG SSFQ +D++GG+S+K++ + FRDSY+DRD
Sbjct: 173 KSSSAPYGSSSFQSADQHGGMSSKRENDSFRDSYKDRD 210
>ref|XP_007222298.1| hypothetical protein PRUPE_ppa003357mg [Prunus persica]
gi|462419234|gb|EMJ23497.1| hypothetical protein
PRUPE_ppa003357mg [Prunus persica]
Length = 582
Score = 145 bits (365), Expect = 8e-33
Identities = 73/101 (72%), Positives = 86/101 (85%), Gaps = 3/101 (2%)
Frame = +1
Query: 1 SLSGFEFVEPNGKDMGINVRKKAETIISLLNDKSKIQEVRNKAAANRDKYVGLSSTGITN 180
+L+ FE+VEPNGKD+GINVRKKAETI++LLN+K KIQEVRNKAAANRDKY GLSSTGIT
Sbjct: 113 ALTSFEYVEPNGKDLGINVRKKAETIVALLNNKDKIQEVRNKAAANRDKYFGLSSTGITY 172
Query: 181 KS---TSASYGGSSFQHSDRYGGVSAKKDGEIFRDSYRDRD 294
KS TSAS+ SS+Q S+RYGG+S G+ F+DSYRDRD
Sbjct: 173 KSGSATSASFSSSSYQSSERYGGLSGTGAGDRFKDSYRDRD 213
>ref|XP_002516482.1| Clathrin interactor, putative [Ricinus communis]
gi|223544302|gb|EEF45823.1| Clathrin interactor,
putative [Ricinus communis]
Length = 562
Score = 144 bits (362), Expect = 2e-32
Identities = 69/98 (70%), Positives = 83/98 (84%)
Frame = +1
Query: 1 SLSGFEFVEPNGKDMGINVRKKAETIISLLNDKSKIQEVRNKAAANRDKYVGLSSTGITN 180
SL+ FE+VEP+GKD+G+NVRKKAE I++LLN+K KIQE RNKAAANRDKYVG+SS+GIT
Sbjct: 100 SLTSFEYVEPSGKDVGLNVRKKAENIVALLNNKDKIQETRNKAAANRDKYVGVSSSGITY 159
Query: 181 KSTSASYGGSSFQHSDRYGGVSAKKDGEIFRDSYRDRD 294
KS SASY G SFQ S RYGG S +D + FRDSY+D+D
Sbjct: 160 KSGSASYSGGSFQSSSRYGGFSGTRDTDNFRDSYKDKD 197
>gb|EXB66907.1| hypothetical protein L484_019545 [Morus notabilis]
Length = 578
Score = 142 bits (358), Expect = 5e-32
Identities = 69/96 (71%), Positives = 80/96 (83%)
Frame = +1
Query: 1 SLSGFEFVEPNGKDMGINVRKKAETIISLLNDKSKIQEVRNKAAANRDKYVGLSSTGITN 180
SLS FE+VEP+GKDMGINVRKK E I++LLN K KIQEVRNKAAANRDKYVGLSS+GIT
Sbjct: 113 SLSSFEYVEPSGKDMGINVRKKVENIVALLNSKDKIQEVRNKAAANRDKYVGLSSSGITF 172
Query: 181 KSTSASYGGSSFQHSDRYGGVSAKKDGEIFRDSYRD 288
KS SASY S+Q SDRYGG+S +DG+ +RD Y +
Sbjct: 173 KSGSASYSSGSYQSSDRYGGLSGTRDGDRYRDQYEE 208
>ref|XP_006340249.1| PREDICTED: clathrin interactor EPSIN 1-like [Solanum tuberosum]
Length = 546
Score = 139 bits (351), Expect = 3e-31
Identities = 68/99 (68%), Positives = 83/99 (83%), Gaps = 1/99 (1%)
Frame = +1
Query: 1 SLSGFEFVEPNGKDMGINVRKKAETIISLLNDKSKIQEVRNKAAANRDKYVGLSSTGITN 180
SL+ FE+VEPNGKDMGINVRKKAE I++LLN+K KIQEVR KA+ANRDKY GLSS+G+T
Sbjct: 113 SLASFEYVEPNGKDMGINVRKKAENIVALLNNKEKIQEVREKASANRDKYFGLSSSGVTV 172
Query: 181 KSTSASYGGS-SFQHSDRYGGVSAKKDGEIFRDSYRDRD 294
KS+S+S+ S +FQ SDRYGG K DG F+DSY+D+D
Sbjct: 173 KSSSSSFSSSGNFQSSDRYGGFGNKNDGNSFKDSYKDKD 211
>ref|XP_004251416.1| PREDICTED: clathrin interactor EPSIN 1-like [Solanum lycopersicum]
Length = 537
Score = 138 bits (347), Expect = 9e-31
Identities = 67/99 (67%), Positives = 83/99 (83%), Gaps = 1/99 (1%)
Frame = +1
Query: 1 SLSGFEFVEPNGKDMGINVRKKAETIISLLNDKSKIQEVRNKAAANRDKYVGLSSTGITN 180
SL+ FE+VEPNGKDMGINVRKKAE I++LLN+K KIQEVR KA+ANRDKY GLSS+G+T
Sbjct: 113 SLASFEYVEPNGKDMGINVRKKAENIVALLNNKEKIQEVREKASANRDKYFGLSSSGVTF 172
Query: 181 KSTSASYGGS-SFQHSDRYGGVSAKKDGEIFRDSYRDRD 294
KS+S+S+ S +FQ SDRYGG K DG F+DSY+++D
Sbjct: 173 KSSSSSFSSSGNFQSSDRYGGFGNKNDGNSFKDSYKEKD 211
>ref|XP_006475977.1| PREDICTED: clathrin interactor EPSIN 1-like isoform X1 [Citrus
sinensis] gi|568844191|ref|XP_006475978.1| PREDICTED:
clathrin interactor EPSIN 1-like isoform X2 [Citrus
sinensis]
Length = 561
Score = 135 bits (340), Expect = 6e-30
Identities = 68/98 (69%), Positives = 81/98 (82%)
Frame = +1
Query: 1 SLSGFEFVEPNGKDMGINVRKKAETIISLLNDKSKIQEVRNKAAANRDKYVGLSSTGITN 180
SLS FE+VEP+GKDMGINVRKKAE I++LLN+K KIQEVRNKA+ANR+KY GLSSTGIT
Sbjct: 113 SLSSFEYVEPSGKDMGINVRKKAENIVALLNNKDKIQEVRNKASANREKYFGLSSTGITY 172
Query: 181 KSTSASYGGSSFQHSDRYGGVSAKKDGEIFRDSYRDRD 294
KS SAS+G ++ SDRYGG G+ FR+SY+DRD
Sbjct: 173 KSGSASFGTGGYRSSDRYGG-----SGDNFRESYKDRD 205
>ref|XP_006450772.1| hypothetical protein CICLE_v10007888mg [Citrus clementina]
gi|557553998|gb|ESR64012.1| hypothetical protein
CICLE_v10007888mg [Citrus clementina]
Length = 507
Score = 135 bits (340), Expect = 6e-30
Identities = 68/98 (69%), Positives = 81/98 (82%)
Frame = +1
Query: 1 SLSGFEFVEPNGKDMGINVRKKAETIISLLNDKSKIQEVRNKAAANRDKYVGLSSTGITN 180
SLS FE+VEP+GKDMGINVRKKAE I++LLN+K KIQEVRNKA+ANR+KY GLSSTGIT
Sbjct: 59 SLSSFEYVEPSGKDMGINVRKKAENIVALLNNKDKIQEVRNKASANREKYFGLSSTGITY 118
Query: 181 KSTSASYGGSSFQHSDRYGGVSAKKDGEIFRDSYRDRD 294
KS SAS+G ++ SDRYGG G+ FR+SY+DRD
Sbjct: 119 KSGSASFGTGGYRSSDRYGG-----SGDNFRESYKDRD 151
>ref|XP_006450769.1| hypothetical protein CICLE_v10007888mg [Citrus clementina]
gi|567917528|ref|XP_006450770.1| hypothetical protein
CICLE_v10007888mg [Citrus clementina]
gi|567917530|ref|XP_006450771.1| hypothetical protein
CICLE_v10007888mg [Citrus clementina]
gi|557553995|gb|ESR64009.1| hypothetical protein
CICLE_v10007888mg [Citrus clementina]
gi|557553996|gb|ESR64010.1| hypothetical protein
CICLE_v10007888mg [Citrus clementina]
gi|557553997|gb|ESR64011.1| hypothetical protein
CICLE_v10007888mg [Citrus clementina]
Length = 561
Score = 135 bits (340), Expect = 6e-30
Identities = 68/98 (69%), Positives = 81/98 (82%)
Frame = +1
Query: 1 SLSGFEFVEPNGKDMGINVRKKAETIISLLNDKSKIQEVRNKAAANRDKYVGLSSTGITN 180
SLS FE+VEP+GKDMGINVRKKAE I++LLN+K KIQEVRNKA+ANR+KY GLSSTGIT
Sbjct: 113 SLSSFEYVEPSGKDMGINVRKKAENIVALLNNKDKIQEVRNKASANREKYFGLSSTGITY 172
Query: 181 KSTSASYGGSSFQHSDRYGGVSAKKDGEIFRDSYRDRD 294
KS SAS+G ++ SDRYGG G+ FR+SY+DRD
Sbjct: 173 KSGSASFGTGGYRSSDRYGG-----SGDNFRESYKDRD 205
>ref|XP_006450767.1| hypothetical protein CICLE_v10007888mg [Citrus clementina]
gi|567917524|ref|XP_006450768.1| hypothetical protein
CICLE_v10007888mg [Citrus clementina]
gi|557553993|gb|ESR64007.1| hypothetical protein
CICLE_v10007888mg [Citrus clementina]
gi|557553994|gb|ESR64008.1| hypothetical protein
CICLE_v10007888mg [Citrus clementina]
Length = 397
Score = 135 bits (340), Expect = 6e-30
Identities = 68/98 (69%), Positives = 81/98 (82%)
Frame = +1
Query: 1 SLSGFEFVEPNGKDMGINVRKKAETIISLLNDKSKIQEVRNKAAANRDKYVGLSSTGITN 180
SLS FE+VEP+GKDMGINVRKKAE I++LLN+K KIQEVRNKA+ANR+KY GLSSTGIT
Sbjct: 113 SLSSFEYVEPSGKDMGINVRKKAENIVALLNNKDKIQEVRNKASANREKYFGLSSTGITY 172
Query: 181 KSTSASYGGSSFQHSDRYGGVSAKKDGEIFRDSYRDRD 294
KS SAS+G ++ SDRYGG G+ FR+SY+DRD
Sbjct: 173 KSGSASFGTGGYRSSDRYGG-----SGDNFRESYKDRD 205
>gb|EYU20387.1| hypothetical protein MIMGU_mgv1a004114mg [Mimulus guttatus]
Length = 543
Score = 134 bits (338), Expect = 1e-29
Identities = 64/98 (65%), Positives = 82/98 (83%)
Frame = +1
Query: 1 SLSGFEFVEPNGKDMGINVRKKAETIISLLNDKSKIQEVRNKAAANRDKYVGLSSTGITN 180
SL+ FE+VEP+GKD+GINVRKKAETI++LLN+K KIQEVR+KAA NR+KY+G+SS+GIT
Sbjct: 113 SLASFEYVEPSGKDLGINVRKKAETIVTLLNNKDKIQEVRSKAATNREKYIGVSSSGITY 172
Query: 181 KSTSASYGGSSFQHSDRYGGVSAKKDGEIFRDSYRDRD 294
KS +AS+ SS + SDRYGG ++D E F DSY D+D
Sbjct: 173 KSGAASFTSSSSKSSDRYGGFGNQRDSETFSDSYNDKD 210
>ref|XP_006355310.1| PREDICTED: clathrin interactor EPSIN 1-like isoform X2 [Solanum
tuberosum]
Length = 551
Score = 134 bits (337), Expect = 1e-29
Identities = 65/100 (65%), Positives = 83/100 (83%), Gaps = 2/100 (2%)
Frame = +1
Query: 1 SLSGFEFVEPNGKDMGINVRKKAETIISLLNDKSKIQEVRNKAAANRDKYVGLSSTGITN 180
SL+ FE+VEPNGKD+GINVRKKAE +++LLN+K KIQEVR+KA ANR+KYVGLSS+GIT
Sbjct: 113 SLTTFEYVEPNGKDVGINVRKKAENVVALLNNKEKIQEVRSKATANREKYVGLSSSGITY 172
Query: 181 KSTSASYGG--SSFQHSDRYGGVSAKKDGEIFRDSYRDRD 294
K++SAS+ S FQ S+RYGG K DG+ F+DSY ++D
Sbjct: 173 KASSASFSSSRSGFQSSERYGGFGNKSDGDSFKDSYNEKD 212
>ref|XP_006355309.1| PREDICTED: clathrin interactor EPSIN 1-like isoform X1 [Solanum
tuberosum]
Length = 552
Score = 134 bits (337), Expect = 1e-29
Identities = 65/100 (65%), Positives = 83/100 (83%), Gaps = 2/100 (2%)
Frame = +1
Query: 1 SLSGFEFVEPNGKDMGINVRKKAETIISLLNDKSKIQEVRNKAAANRDKYVGLSSTGITN 180
SL+ FE+VEPNGKD+GINVRKKAE +++LLN+K KIQEVR+KA ANR+KYVGLSS+GIT
Sbjct: 113 SLTTFEYVEPNGKDVGINVRKKAENVVALLNNKEKIQEVRSKATANREKYVGLSSSGITY 172
Query: 181 KSTSASYGG--SSFQHSDRYGGVSAKKDGEIFRDSYRDRD 294
K++SAS+ S FQ S+RYGG K DG+ F+DSY ++D
Sbjct: 173 KASSASFSSSRSGFQSSERYGGFGNKSDGDSFKDSYNEKD 212
>ref|XP_003550292.1| PREDICTED: clathrin interactor EPSIN 1 isoform 1 [Glycine max]
Length = 564
Score = 134 bits (336), Expect = 2e-29
Identities = 70/99 (70%), Positives = 84/99 (84%), Gaps = 2/99 (2%)
Frame = +1
Query: 1 SLSGFEFVEPNGKDMGINVRKKAETIISLLNDKSKIQEVRNKAAANRDKYVGLSSTGITN 180
+LS FE+VEP+GKD+G+NVRKKAE I+SLLNDK KI EVRNKAAANRDKY+G+SS+GIT
Sbjct: 113 ALSSFEYVEPSGKDVGLNVRKKAENIVSLLNDKDKIHEVRNKAAANRDKYIGVSSSGITY 172
Query: 181 KSTSA-SYG-GSSFQHSDRYGGVSAKKDGEIFRDSYRDR 291
KS SA SYG GSSFQ S +YGG + +DG+ F DSYRD+
Sbjct: 173 KSGSASSYGSGSSFQSSGKYGGFGS-RDGDRFNDSYRDK 210
>ref|XP_003544513.1| PREDICTED: clathrin interactor EPSIN 1-like isoform 1 [Glycine max]
Length = 564
Score = 134 bits (336), Expect = 2e-29
Identities = 70/99 (70%), Positives = 84/99 (84%), Gaps = 2/99 (2%)
Frame = +1
Query: 1 SLSGFEFVEPNGKDMGINVRKKAETIISLLNDKSKIQEVRNKAAANRDKYVGLSSTGITN 180
+LS FE+VEP+GKD+G+NVRKKAE I+SLLNDK KI EVRNKAAANRDKY+G+SS+GIT
Sbjct: 113 ALSSFEYVEPSGKDVGLNVRKKAENIVSLLNDKDKIHEVRNKAAANRDKYIGVSSSGITY 172
Query: 181 KSTSA-SYG-GSSFQHSDRYGGVSAKKDGEIFRDSYRDR 291
KS SA SYG GSSFQ S +YGG + +DG+ F DSYRD+
Sbjct: 173 KSGSASSYGSGSSFQSSGKYGGFGS-RDGDRFNDSYRDK 210
>gb|EYU29308.1| hypothetical protein MIMGU_mgv1a004074mg [Mimulus guttatus]
Length = 545
Score = 132 bits (333), Expect = 4e-29
Identities = 65/101 (64%), Positives = 82/101 (81%), Gaps = 3/101 (2%)
Frame = +1
Query: 1 SLSGFEFVEPNGKDMGINVRKKAETIISLLNDKSKIQEVRNKAAANRDKYVGLSSTGITN 180
SLS FE+VEPNGKD+GINVRKKAETI++LLNDK K+QEVRNKAA NR+KY+GLSS+G+T
Sbjct: 113 SLSTFEYVEPNGKDLGINVRKKAETIVALLNDKDKMQEVRNKAATNREKYIGLSSSGMTY 172
Query: 181 KSTSASYGGSSFQHSDRYGGVSAKKDGEIFR---DSYRDRD 294
KS + S+ S+F+ +DRYGG + D E + DSYRD+D
Sbjct: 173 KSGAPSFSSSNFKSNDRYGGFGNRGDSETSKTSNDSYRDKD 213
>ref|XP_007012223.1| ENTH/VHS family protein [Theobroma cacao]
gi|508782586|gb|EOY29842.1| ENTH/VHS family protein
[Theobroma cacao]
Length = 561
Score = 132 bits (333), Expect = 4e-29
Identities = 65/99 (65%), Positives = 81/99 (81%), Gaps = 1/99 (1%)
Frame = +1
Query: 1 SLSGFEFVEPNGKDMGINVRKKAETIISLLNDKSKIQEVRNKAAANRDKYVGLSSTGITN 180
SL+ FE+VEP+GKD+G+NVRKKAETI+ LLN+K KIQE RNKAAANRDKYVGLSSTGIT
Sbjct: 113 SLTSFEYVEPSGKDVGLNVRKKAETIVGLLNNKEKIQEARNKAAANRDKYVGLSSTGITY 172
Query: 181 KSTSASY-GGSSFQHSDRYGGVSAKKDGEIFRDSYRDRD 294
KS +ASY G DRYGG+S ++ + +RDSY+++D
Sbjct: 173 KSGAASYTSGGYHGGGDRYGGLSTTRESDSYRDSYKEKD 211
>ref|XP_004245127.1| PREDICTED: clathrin interactor EPSIN 1-like [Solanum lycopersicum]
Length = 553
Score = 132 bits (331), Expect = 7e-29
Identities = 64/100 (64%), Positives = 82/100 (82%), Gaps = 2/100 (2%)
Frame = +1
Query: 1 SLSGFEFVEPNGKDMGINVRKKAETIISLLNDKSKIQEVRNKAAANRDKYVGLSSTGITN 180
SL+ FE+VEPNGKD+GINVRKKAE +++LLN+K KIQEVR+KA ANR+KYVGLSS+GIT
Sbjct: 115 SLTTFEYVEPNGKDVGINVRKKAENVVALLNNKEKIQEVRSKATANREKYVGLSSSGITY 174
Query: 181 KSTSASYGG--SSFQHSDRYGGVSAKKDGEIFRDSYRDRD 294
K++SAS+ S Q S+RYGG K DG+ F+DSY ++D
Sbjct: 175 KASSASFSSSRSGLQSSERYGGFGNKSDGDSFKDSYNEKD 214