BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Akebia25_contig00025914
         (1806 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_007050677.1| Debranching enzyme 1 [Theobroma cacao] gi|50...    91   3e-27
ref|XP_007201750.1| hypothetical protein PRUPE_ppa001199mg [Prun...    89   2e-26
ref|XP_006303226.1| hypothetical protein CARUB_v10012076mg [Caps...    84   2e-26
gb|AAM98123.1| putative isoamylase [Arabidopsis thaliana]              87   7e-26
ref|NP_171830.1| Isoamylase 2 [Arabidopsis thaliana] gi|42571321...    87   7e-26
ref|XP_002889452.1| ATISA2/BE2/DBE1/ISA2 [Arabidopsis lyrata sub...    82   7e-26
ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Viti...    86   2e-25
ref|XP_004164565.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2...    79   4e-25
ref|XP_004136188.1| PREDICTED: isoamylase 2, chloroplastic-like ...    79   4e-25
ref|XP_006846640.1| hypothetical protein AMTR_s00156p00067780 [A...    84   2e-24
ref|XP_006655289.1| PREDICTED: isoamylase 2, chloroplastic-like,...    67   3e-24
ref|XP_004292603.1| PREDICTED: isoamylase 2, chloroplastic-like ...    80   8e-24
ref|XP_006418247.1| hypothetical protein EUTSA_v10006762mg [Eutr...    77   1e-23
gb|EYU33286.1| hypothetical protein MIMGU_mgv1a001869mg [Mimulus...    81   3e-23
ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|2...    78   7e-23
ref|XP_002441051.1| hypothetical protein SORBIDRAFT_09g019480 [S...    66   1e-22
ref|XP_006386822.1| hypothetical protein POPTR_0002s22530g [Popu...    77   3e-22
ref|NP_001105666.1| isoamylase-type starch debranching enzyme IS...    66   4e-22
gb|ACG48178.1| isoamylase-type starch debranching enzyme ISO2 [Z...    66   4e-22
gb|EAY96723.1| hypothetical protein OsI_18641 [Oryza sativa Indi...    65   1e-21

>ref|XP_007050677.1| Debranching enzyme 1 [Theobroma cacao] gi|508702938|gb|EOX94834.1|
            Debranching enzyme 1 [Theobroma cacao]
          Length = 867

 Score = 90.9 bits (224), Expect(2) = 3e-27
 Identities = 71/206 (34%), Positives = 99/206 (48%), Gaps = 23/206 (11%)
 Frame = +3

Query: 969  LYAIDNLSYSVFDKGVE*EARSALNCNNHVAM*IAWDSLCHWMTYFQFEWLCLMNASLLL 1148
            L  +D+LSY   ++  + E R+ALNCN  V   +  DSL HW+T F  +  C +NAS LL
Sbjct: 467  LQGLDDLSYYYRNRVQDLERRNALNCNYPVVQRMILDSLRHWVTEFHIDGFCFINASCLL 526

Query: 1149 GGSNNED---------VPFD*VNLISDTKIFADCRDPCVMSPKEICFPHLKRW------- 1280
             G   E          + FD   L+S TKI ADC  P  M PKEI FPH K+W       
Sbjct: 527  RGFYGEHLSRPPLIEAIAFD--PLLSKTKIIADCWYPHDMMPKEIRFPHWKKWAEMNTKF 584

Query: 1281 LT*IHDLVRMKENLEGGVYSS*TKMACGNGYVFLGGWPHNFFYFYCKELWTTP*GLGLLS 1460
             T I + +R +     G  SS     CG+G +F  G    F + Y  + +  P    +  
Sbjct: 585  CTDIRNFLRGE-----GALSSLATRLCGSGDIFSDGRGPAFSFNYIAKNFGLPLVDLVSF 639

Query: 1461 *QCTLSNQV-------GPANKTGVLE 1517
             +  +++++       GP NKT VLE
Sbjct: 640  SKAEIASELSWNCGVEGPTNKTAVLE 665



 Score = 60.1 bits (144), Expect(2) = 3e-27
 Identities = 41/98 (41%), Positives = 51/98 (52%), Gaps = 14/98 (14%)
 Frame = +2

Query: 680 FTKDKSSQPPTDVVGTLFWFGN-FHHLKXXXXXXXXXXXFPFMKKKKRGPYFSYHF---- 844
           FT+DKSS+ P DVVGT         HLK             F   +++GPYF  HF    
Sbjct: 364 FTEDKSSKLPADVVGTFAGVTEKVQHLKDLGVNAVLLEPI-FTFDEQKGPYFPCHFFSPT 422

Query: 845 ---------LSAI*SMKEMIKDFHAYNVEVLLEVGFTH 931
                    +SAI S+KEM+K+ HA  +EVLLEV FTH
Sbjct: 423 NLYGPSNGSVSAINSIKEMVKNLHANGMEVLLEVVFTH 460


>ref|XP_007201750.1| hypothetical protein PRUPE_ppa001199mg [Prunus persica]
            gi|462397150|gb|EMJ02949.1| hypothetical protein
            PRUPE_ppa001199mg [Prunus persica]
          Length = 883

 Score = 88.6 bits (218), Expect(2) = 2e-26
 Identities = 72/201 (35%), Positives = 92/201 (45%), Gaps = 18/201 (8%)
 Frame = +3

Query: 969  LYAIDNLSYSVFDKGVE*EARSALNCNNHVAM*IAWDSLCHWMTYFQFEWLCLMNASLLL 1148
            L  ID  SY   ++  + EAR+ALNCN  +   +  DSL +W+T F  +    +NAS LL
Sbjct: 484  LQGIDISSYYHVNEVEDLEARNALNCNYPIVQQLVLDSLRYWVTEFHVDGFFFINASSLL 543

Query: 1149 GGSNN---------EDVPFD*VNLISDTKIFADCRDPCVMSPKEICFPHLKRWLT*IHDL 1301
             G N          E + FD   L+S TKI ADC DP  M+PKE  FPH +RW       
Sbjct: 544  RGFNGEYLSRPPLVEAIAFD--PLLSKTKIIADCWDPHGMAPKETHFPHWRRWAEVNTKF 601

Query: 1302 VRMKEN-LEG-GVYSS*TKMACGNGYVFLGGWPHNFFYFYCKELWTTP-------*GLGL 1454
                 N L G G+ S      CGNG +F  G    F + +       P        G+ L
Sbjct: 602  CNDVRNFLRGEGLLSDLATRLCGNGDIFSDGRGPAFAFNFISRNSGLPLVDLVSFSGVEL 661

Query: 1455 LS*QCTLSNQVGPANKTGVLE 1517
             S       + GP NKT VLE
Sbjct: 662  ASELSWNCGEEGPTNKTAVLE 682



 Score = 59.7 bits (143), Expect(2) = 2e-26
 Identities = 42/104 (40%), Positives = 54/104 (51%), Gaps = 15/104 (14%)
 Frame = +2

Query: 665 LIVECFTKDKSSQPPTDVVGTLFWFGN-FHHLKXXXXXXXXXXX-FPFMKKKKRGPYFSY 838
           L V  FT+ KSSQ PT++ G+         H K            FPF ++K  GPYF +
Sbjct: 376 LNVTRFTEHKSSQLPTNIGGSFSGLTEKLEHFKDLGVNAVLLEPIFPFDEQK--GPYFPH 433

Query: 839 HF-------------LSAI*SMKEMIKDFHAYNVEVLLEVGFTH 931
           HF             +SA+ SMKEM++ FHA  +EVLLEV FTH
Sbjct: 434 HFFSPMDCFGPSRGPVSAVNSMKEMVRKFHANGIEVLLEVVFTH 477


>ref|XP_006303226.1| hypothetical protein CARUB_v10012076mg [Capsella rubella]
            gi|482571937|gb|EOA36124.1| hypothetical protein
            CARUB_v10012076mg [Capsella rubella]
          Length = 882

 Score = 84.3 bits (207), Expect(3) = 2e-26
 Identities = 57/165 (34%), Positives = 81/165 (49%), Gaps = 16/165 (9%)
 Frame = +3

Query: 969  LYAIDNLSYSVFDKGVE*EARSALNCNNHVAM*IAWDSLCHWMTYFQFEWLCLMNASLLL 1148
            L  ID+ SY    +  + +++S LNCN  V   +  +SL +W+T F  +  C +NAS LL
Sbjct: 476  LRGIDDSSYYYKGRANDLDSKSYLNCNYPVVQQLILESLRYWVTEFHIDGFCFINASSLL 535

Query: 1149 GGSNNED---------VPFD*VNLISDTKIFADCRDPCVMSPKEICFPHLKRW------- 1280
             G + E          + FD   L+++TK+ ADC DP  M PKE+ FPH KRW       
Sbjct: 536  RGVHGEQLSRPPLVEAIAFD--PLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRY 593

Query: 1281 LT*IHDLVRMKENLEGGVYSS*TKMACGNGYVFLGGWPHNFFYFY 1415
               I + +R K     GV S      CG+G +F  G    F + Y
Sbjct: 594  CQNIRNFMRGK-----GVLSDLATRICGSGDIFTDGRGPAFSFNY 633



 Score = 57.4 bits (137), Expect(3) = 2e-26
 Identities = 39/103 (37%), Positives = 52/103 (50%), Gaps = 14/103 (13%)
 Frame = +2

Query: 665 LIVECFTKDKSSQPPTDVVGTLFWFGN-FHHLKXXXXXXXXXXXFPFMKKKKRGPYFSYH 841
           L V+ FT+ KSS+ P++V GT        +HLK             F   +++GPYF  H
Sbjct: 368 LNVKGFTQHKSSKLPSNVTGTFSGVAEKVNHLKTLGINAVLLEPI-FSFSEQKGPYFPVH 426

Query: 842 FLS-------------AI*SMKEMIKDFHAYNVEVLLEVGFTH 931
           F S             A+ SMKEM+K  H+  +EVLLEV FTH
Sbjct: 427 FFSPMDMYGPSNGFESAVKSMKEMVKKLHSQGIEVLLEVVFTH 469



 Score = 26.6 bits (57), Expect(3) = 2e-26
 Identities = 14/29 (48%), Positives = 17/29 (58%)
 Frame = +1

Query: 1390 GLTISFIFIARNFGLLLEDLVCFRNNALS 1476
            G   SF +I+RN GL L DLV F    L+
Sbjct: 626  GPAFSFNYISRNSGLSLVDLVSFSGPELA 654


>gb|AAM98123.1| putative isoamylase [Arabidopsis thaliana]
          Length = 882

 Score = 86.7 bits (213), Expect(3) = 7e-26
 Identities = 59/160 (36%), Positives = 80/160 (50%), Gaps = 11/160 (6%)
 Frame = +3

Query: 969  LYAIDNLSYSVFDKGVE*EARSALNCNNHVAM*IAWDSLCHWMTYFQFEWLCLMNASLLL 1148
            L  ID+ SY    +  + +++S LNCN  V   +  +SL +W+T F  +  C +NAS LL
Sbjct: 477  LRGIDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLL 536

Query: 1149 GGSNNED---------VPFD*VNLISDTKIFADCRDPCVMSPKEICFPHLKRWLT*IHDL 1301
             G + E          + FD   L+++TK+ ADC DP  M PKE+ FPH KRW       
Sbjct: 537  RGVHGEQLSRPPLVEAIAFD--PLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRY 594

Query: 1302 VRMKEN-LEG-GVYSS*TKMACGNGYVFLGGWPHNFFYFY 1415
             R   N L G GV S      CG+G VF  G    F + Y
Sbjct: 595  CRNVRNFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNY 634



 Score = 53.9 bits (128), Expect(3) = 7e-26
 Identities = 37/103 (35%), Positives = 51/103 (49%), Gaps = 14/103 (13%)
 Frame = +2

Query: 665 LIVECFTKDKSSQPPTDVVGTLFWFGN-FHHLKXXXXXXXXXXXFPFMKKKKRGPYFSYH 841
           L V+ FT+ +SS+ P++V GT         HLK             F   +++GPYF +H
Sbjct: 369 LNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPI-FSFSEQKGPYFPFH 427

Query: 842 FLS-------------AI*SMKEMIKDFHAYNVEVLLEVGFTH 931
           F S             A+ SMK M+K  H+  +EVLLEV FTH
Sbjct: 428 FFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTH 470



 Score = 25.8 bits (55), Expect(3) = 7e-26
 Identities = 13/29 (44%), Positives = 17/29 (58%)
 Frame = +1

Query: 1390 GLTISFIFIARNFGLLLEDLVCFRNNALS 1476
            G   SF +I+RN GL L D+V F    L+
Sbjct: 627  GPAFSFNYISRNSGLSLVDIVSFSGPELA 655


>ref|NP_171830.1| Isoamylase 2 [Arabidopsis thaliana] gi|42571321|ref|NP_973751.1|
            Isoamylase 2 [Arabidopsis thaliana]
            gi|251764780|sp|Q8L735.2|ISOA2_ARATH RecName:
            Full=Isoamylase 2, chloroplastic; Short=AtISA2; AltName:
            Full=Protein DEBRANCHING ENZYME 1; Flags: Precursor
            gi|3850573|gb|AAC72113.1| Similar to gi|1652733 glycogen
            operon protein GlgX from Synechocystis sp. genome
            gb|D90908. ESTs gb|H36690, gb|AA712462, gb|AA651230 and
            gb|N95932 come from this gene [Arabidopsis thaliana]
            gi|332189436|gb|AEE27557.1| Isoamylase 2 [Arabidopsis
            thaliana] gi|332189437|gb|AEE27558.1| Isoamylase 2
            [Arabidopsis thaliana]
          Length = 882

 Score = 86.7 bits (213), Expect(3) = 7e-26
 Identities = 59/160 (36%), Positives = 80/160 (50%), Gaps = 11/160 (6%)
 Frame = +3

Query: 969  LYAIDNLSYSVFDKGVE*EARSALNCNNHVAM*IAWDSLCHWMTYFQFEWLCLMNASLLL 1148
            L  ID+ SY    +  + +++S LNCN  V   +  +SL +W+T F  +  C +NAS LL
Sbjct: 477  LRGIDDSSYYYKGRANDLDSKSYLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLL 536

Query: 1149 GGSNNED---------VPFD*VNLISDTKIFADCRDPCVMSPKEICFPHLKRWLT*IHDL 1301
             G + E          + FD   L+++TK+ ADC DP  M PKE+ FPH KRW       
Sbjct: 537  RGVHGEQLSRPPLVEAIAFD--PLLAETKLIADCWDPLEMMPKEVRFPHWKRWAELNTRY 594

Query: 1302 VRMKEN-LEG-GVYSS*TKMACGNGYVFLGGWPHNFFYFY 1415
             R   N L G GV S      CG+G VF  G    F + Y
Sbjct: 595  CRNVRNFLRGRGVLSDLATRICGSGDVFTDGRGPAFSFNY 634



 Score = 53.9 bits (128), Expect(3) = 7e-26
 Identities = 37/103 (35%), Positives = 51/103 (49%), Gaps = 14/103 (13%)
 Frame = +2

Query: 665 LIVECFTKDKSSQPPTDVVGTLFWFGN-FHHLKXXXXXXXXXXXFPFMKKKKRGPYFSYH 841
           L V+ FT+ +SS+ P++V GT         HLK             F   +++GPYF +H
Sbjct: 369 LNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAVLLEPI-FSFSEQKGPYFPFH 427

Query: 842 FLS-------------AI*SMKEMIKDFHAYNVEVLLEVGFTH 931
           F S             A+ SMK M+K  H+  +EVLLEV FTH
Sbjct: 428 FFSPMDIYGPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTH 470



 Score = 25.8 bits (55), Expect(3) = 7e-26
 Identities = 13/29 (44%), Positives = 17/29 (58%)
 Frame = +1

Query: 1390 GLTISFIFIARNFGLLLEDLVCFRNNALS 1476
            G   SF +I+RN GL L D+V F    L+
Sbjct: 627  GPAFSFNYISRNSGLSLVDIVSFSGPELA 655


>ref|XP_002889452.1| ATISA2/BE2/DBE1/ISA2 [Arabidopsis lyrata subsp. lyrata]
            gi|297335294|gb|EFH65711.1| ATISA2/BE2/DBE1/ISA2
            [Arabidopsis lyrata subsp. lyrata]
          Length = 872

 Score = 81.6 bits (200), Expect(3) = 7e-26
 Identities = 55/160 (34%), Positives = 79/160 (49%), Gaps = 11/160 (6%)
 Frame = +3

Query: 969  LYAIDNLSYSVFDKGVE*EARSALNCNNHVAM*IAWDSLCHWMTYFQFEWLCLMNASLLL 1148
            L+ ID+  Y    +  + + +S LNCN  V   +  +SL +W+T F  +  C +NAS LL
Sbjct: 466  LHGIDDSCYYYKGRANDLDYKSYLNCNYPVVQQLILESLRYWVTEFHVDGFCFINASSLL 525

Query: 1149 GGSNNED---------VPFD*VNLISDTKIFADCRDPCVMSPKEICFPHLKRWLT*IHDL 1301
             G + E          + FD   L+++TK+ +DC DP  M PKE+ FPH K+W       
Sbjct: 526  RGVHGEQLSRPPLVEAITFD--PLLAETKLISDCWDPLEMMPKEVRFPHWKQWAELNTRY 583

Query: 1302 VRMKEN-LEG-GVYSS*TKMACGNGYVFLGGWPHNFFYFY 1415
             R   N L G GV S      CG+G +F  G    F + Y
Sbjct: 584  CRNVRNFLRGRGVLSDLATRICGSGDIFTDGRGPAFSFNY 623



 Score = 58.2 bits (139), Expect(3) = 7e-26
 Identities = 39/103 (37%), Positives = 52/103 (50%), Gaps = 14/103 (13%)
 Frame = +2

Query: 665 LIVECFTKDKSSQPPTDVVGTLFWFGN-FHHLKXXXXXXXXXXXFPFMKKKKRGPYFSYH 841
           L V+ FT+ KSS+ P++V GT         HLK             F   +++GPYF +H
Sbjct: 358 LNVKGFTQHKSSKLPSNVAGTFSGVAEKVSHLKTLGVNGVLLEPI-FSFSEQKGPYFPFH 416

Query: 842 FLS-------------AI*SMKEMIKDFHAYNVEVLLEVGFTH 931
           F S             A+ SMKEM+K  H+  +EVLLEV FTH
Sbjct: 417 FFSPMDIYGPSNSLESAVNSMKEMVKKLHSQGIEVLLEVVFTH 459



 Score = 26.6 bits (57), Expect(3) = 7e-26
 Identities = 14/29 (48%), Positives = 17/29 (58%)
 Frame = +1

Query: 1390 GLTISFIFIARNFGLLLEDLVCFRNNALS 1476
            G   SF +I+RN GL L DLV F    L+
Sbjct: 616  GPAFSFNYISRNSGLSLVDLVSFSGPELA 644


>ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Vitis vinifera]
          Length = 882

 Score = 86.3 bits (212), Expect(2) = 2e-25
 Identities = 72/205 (35%), Positives = 92/205 (44%), Gaps = 22/205 (10%)
 Frame = +3

Query: 969  LYAIDNLSYSVFDKGVE*EARSALNCNNHVAM*IAWDSLCHWMTYFQFEWLCLMNASLLL 1148
            L  ID+  Y   +   +   R+ALNCN  +   +  DSL +W+T F  +  C +NAS LL
Sbjct: 483  LQGIDDSCYYYVNGDADLGIRNALNCNYSIVQQMIVDSLRYWVTEFHVDGFCFINASSLL 542

Query: 1149 GGSNNE---------DVPFD*VNLISDTKIFADCRDPCVMSPKEICFPHLKRWLT*IHDL 1301
             G + E          + FD   L+S TKI ADC DP  M PKEI FPH KRW       
Sbjct: 543  RGFHGEYLSRPPLVETIAFD--PLLSKTKIIADCWDPRNMLPKEIRFPHWKRWAEVNTRF 600

Query: 1302 VRMKEN-LEGGVYSS*TKMACGNGYVFLGG-WPHNFFYFYCKELWTTP*GLGLLS*QCTL 1475
                 N L G   S      CG+G +F+ G  P   F F  K       GL L+      
Sbjct: 601  CNDVRNFLRGEGLSDFATRLCGSGDIFMDGRGPAFSFNFTTKNF-----GLPLVDLVSFS 655

Query: 1476 SNQV-----------GPANKTGVLE 1517
            S+++           GP NKT VLE
Sbjct: 656  SSELASELSWNCGDEGPTNKTTVLE 680



 Score = 58.9 bits (141), Expect(2) = 2e-25
 Identities = 38/98 (38%), Positives = 49/98 (50%), Gaps = 14/98 (14%)
 Frame = +2

Query: 680 FTKDKSSQPPTDVVGTLFWF-GNFHHLKXXXXXXXXXXXFPFMKKKKRGPYFSYHF---- 844
           FTKD+SSQ  +D+ GT        HH K             F   +++GPYF +HF    
Sbjct: 380 FTKDESSQVASDLAGTFSGLMEKLHHFKDLGVNAVLLEPI-FSFDEQKGPYFPFHFFSPM 438

Query: 845 ---------LSAI*SMKEMIKDFHAYNVEVLLEVGFTH 931
                    +S I S+KEM+K  HA  +EVLLEV FTH
Sbjct: 439 NVYGPSSGPVSTINSVKEMVKRLHANGIEVLLEVVFTH 476


>ref|XP_004164565.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2, chloroplastic-like
            [Cucumis sativus]
          Length = 885

 Score = 79.0 bits (193), Expect(2) = 4e-25
 Identities = 57/159 (35%), Positives = 76/159 (47%), Gaps = 16/159 (10%)
 Frame = +3

Query: 960  NIGLYAIDNLSYSVFDKGVE*EARSALNCNNHVAM*IAWDSLCHWMTYFQFEWLCLMNAS 1139
            N  L  ID+ SY   ++    E +SALNCN  +   +  DSL +W+T F  +  C +NAS
Sbjct: 484  NGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNAS 543

Query: 1140 LLLGGSNN---------EDVPFD*VNLISDTKIFADCRDPCVMSPKEICFPHLKRW---- 1280
             LL G +          E + FD   L+S TK+ AD  DP  +  KE  FPH KRW    
Sbjct: 544  FLLRGHHGELLSRPPFVEAIAFD--PLLSKTKLVADFWDPQELESKETRFPHWKRWAEVN 601

Query: 1281 ---LT*IHDLVRMKENLEGGVYSS*TKMACGNGYVFLGG 1388
                + I D  R +     G+ SS     CG+G VF  G
Sbjct: 602  SKFCSDIRDFFRGE-----GLISSLATRLCGSGDVFSDG 635



 Score = 64.7 bits (156), Expect(2) = 4e-25
 Identities = 43/104 (41%), Positives = 51/104 (49%), Gaps = 15/104 (14%)
 Frame = +2

Query: 665 LIVECFTKDKSSQPPTDVVGTLFWFGN--FHHLKXXXXXXXXXXXFPFMKKKKRGPYFSY 838
           L VE FT DKSSQ P D+ GT         H              F F   +K GPYF +
Sbjct: 379 LNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLLEPIFQF--DEKEGPYFPF 436

Query: 839 HF-------------LSAI*SMKEMIKDFHAYNVEVLLEVGFTH 931
           HF             +SAI SMKEM+K+ HA  VEV+LEV +TH
Sbjct: 437 HFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480


>ref|XP_004136188.1| PREDICTED: isoamylase 2, chloroplastic-like [Cucumis sativus]
          Length = 885

 Score = 79.0 bits (193), Expect(2) = 4e-25
 Identities = 57/159 (35%), Positives = 76/159 (47%), Gaps = 16/159 (10%)
 Frame = +3

Query: 960  NIGLYAIDNLSYSVFDKGVE*EARSALNCNNHVAM*IAWDSLCHWMTYFQFEWLCLMNAS 1139
            N  L  ID+ SY   ++    E +SALNCN  +   +  DSL +W+T F  +  C +NAS
Sbjct: 484  NGALQGIDDSSYYFTNRVANLEEKSALNCNYPIVQQLLLDSLRYWVTEFHVDGFCFVNAS 543

Query: 1140 LLLGGSNN---------EDVPFD*VNLISDTKIFADCRDPCVMSPKEICFPHLKRW---- 1280
             LL G +          E + FD   L+S TK+ AD  DP  +  KE  FPH KRW    
Sbjct: 544  FLLRGHHGELLSRPPFVEAIAFD--PLLSKTKLVADFWDPQELESKETRFPHWKRWAEVN 601

Query: 1281 ---LT*IHDLVRMKENLEGGVYSS*TKMACGNGYVFLGG 1388
                + I D  R +     G+ SS     CG+G VF  G
Sbjct: 602  SKFCSDIRDFFRGE-----GLISSLATRLCGSGDVFSDG 635



 Score = 64.7 bits (156), Expect(2) = 4e-25
 Identities = 43/104 (41%), Positives = 51/104 (49%), Gaps = 15/104 (14%)
 Frame = +2

Query: 665 LIVECFTKDKSSQPPTDVVGTLFWFGN--FHHLKXXXXXXXXXXXFPFMKKKKRGPYFSY 838
           L VE FT DKSSQ P D+ GT         H              F F   +K GPYF +
Sbjct: 379 LNVERFTMDKSSQLPADIAGTFSGLTKKLLHFKNLGVNAVLLEPIFQF--DEKEGPYFPF 436

Query: 839 HF-------------LSAI*SMKEMIKDFHAYNVEVLLEVGFTH 931
           HF             +SAI SMKEM+K+ HA  VEV+LEV +TH
Sbjct: 437 HFFSPTNNYGPSGASISAINSMKEMVKELHANGVEVILEVVYTH 480


>ref|XP_006846640.1| hypothetical protein AMTR_s00156p00067780 [Amborella trichopoda]
            gi|548849492|gb|ERN08315.1| hypothetical protein
            AMTR_s00156p00067780 [Amborella trichopoda]
          Length = 926

 Score = 84.3 bits (207), Expect(2) = 2e-24
 Identities = 56/171 (32%), Positives = 81/171 (47%), Gaps = 11/171 (6%)
 Frame = +3

Query: 936  DDGDCALSNIGLYAIDNLSYSVFDKGVE*EARSALNCNNHVAM*IAWDSLCHWMTYFQFE 1115
            + GD     I    IDN SY + D+ VE E  + LNCN+ +   +  D L HW+  +  +
Sbjct: 520  EGGDSLCQTISFRGIDNSSYYIVDRNVESEGGNVLNCNHPMVQTMILDCLRHWVHEYHVD 579

Query: 1116 WLCLMNASLLLGGSNN---------EDVPFD*VNLISDTKIFADCRDPCVMSPKEICFPH 1268
              C +N+S L  GS+          E + FD   ++S  KI ADC  P  M  KEI FPH
Sbjct: 580  GFCFINSSSLAKGSDGELLTLSPLIEAIAFD--PILSHAKIIADCWSPLDMQCKEIHFPH 637

Query: 1269 LKRWL-T*IHDLVRMKENLEG-GVYSS*TKMACGNGYVFLGGWPHNFFYFY 1415
             K+W          ++  L G G+ S+     CG+G +F  G   +F + Y
Sbjct: 638  WKKWAEMNARFCYDVRNFLRGEGLLSNLATRLCGSGDIFSDGRGPSFSFNY 688



 Score = 57.0 bits (136), Expect(2) = 2e-24
 Identities = 40/99 (40%), Positives = 49/99 (49%), Gaps = 15/99 (15%)
 Frame = +2

Query: 680 FTKDKSSQPPTDVVGTLFW-FGNFHHLKXXXXXXXXXXX-FPFMKKKKRGPYFSYHF--- 844
           FT+D SS  P D+ GT        HHLK            FPF + K  GPY+ Y+F   
Sbjct: 421 FTEDMSSLLPKDIAGTFLGVIQKLHHLKNLGVNALLLEPIFPFDELK--GPYYPYNFFAP 478

Query: 845 ----------LSAI*SMKEMIKDFHAYNVEVLLEVGFTH 931
                     +SA  SMKEM+K  HA  +EVLLE+ FTH
Sbjct: 479 MNKYGPLRDGISACTSMKEMVKALHANGLEVLLEMVFTH 517


>ref|XP_006655289.1| PREDICTED: isoamylase 2, chloroplastic-like, partial [Oryza
            brachyantha]
          Length = 703

 Score = 67.0 bits (162), Expect(4) = 3e-24
 Identities = 47/125 (37%), Positives = 61/125 (48%), Gaps = 9/125 (7%)
 Frame = +3

Query: 933  TDDGDCALSNIGLYAIDNLSYSVFDKGVE*EARSALNCNNHVAM*IAWDSLCHWMTYFQF 1112
            T +G+     I +  IDN SY + + G+     S LNCN  V   +  DSL HW+  F  
Sbjct: 297  TAEGESECQTISMRGIDNPSYYIAN-GMAGCKASVLNCNQPVTQRLILDSLRHWVLDFHV 355

Query: 1113 EWLCLMNASLLL---GGSN------NEDVPFD*VNLISDTKIFADCRDPCVMSPKEICFP 1265
            +  C +NA  LL   GG         E + FD V  +SDTKI AD   P  +S  +  FP
Sbjct: 356  DGFCFINAPFLLRDPGGEYLSRPPLLEAIAFDPV--LSDTKIIADPWSPLDISNVQFLFP 413

Query: 1266 HLKRW 1280
            H KRW
Sbjct: 414  HWKRW 418



 Score = 60.1 bits (144), Expect(4) = 3e-24
 Identities = 42/102 (41%), Positives = 49/102 (48%), Gaps = 15/102 (14%)
 Frame = +2

Query: 671 VECFTKDKSSQPPTDVVGTLFWF-GNFHHLKXXXXXXXXXXX-FPFMKKKKRGPYFSYHF 844
           V  FTKDKSS  P D  GT   F     H +            FPF + K  GPYF YHF
Sbjct: 197 VALFTKDKSSGLPDDAAGTFSGFCAKIEHFRSLGVNAILLEPVFPFHQVK--GPYFPYHF 254

Query: 845 -------------LSAI*SMKEMIKDFHAYNVEVLLEVGFTH 931
                        +SAI SMK+++K  H   +EVLLEV FTH
Sbjct: 255 FSPMNLYSSEGSSVSAINSMKDIVKAMHKNGIEVLLEVVFTH 296



 Score = 31.2 bits (69), Expect(4) = 3e-24
 Identities = 17/40 (42%), Positives = 22/40 (55%)
 Frame = +1

Query: 1357 LVGMGMFSWVAGLTISFIFIARNFGLLLEDLVCFRNNALS 1476
            L G G      G   SF +++RN GL L DLV F N+ L+
Sbjct: 446  LCGSGDLFSTRGPAFSFNYVSRNSGLSLVDLVSFSNDDLA 485



 Score = 22.3 bits (46), Expect(4) = 3e-24
 Identities = 11/22 (50%), Positives = 13/22 (59%)
 Frame = +2

Query: 1256 LFSPLEKMVNMNT*FSEDERKF 1321
            LF   ++   MNT FS D RKF
Sbjct: 411  LFPHWKRWAEMNTRFSIDVRKF 432


>ref|XP_004292603.1| PREDICTED: isoamylase 2, chloroplastic-like [Fragaria vesca subsp.
            vesca]
          Length = 868

 Score = 80.5 bits (197), Expect(2) = 8e-24
 Identities = 69/201 (34%), Positives = 87/201 (43%), Gaps = 18/201 (8%)
 Frame = +3

Query: 969  LYAIDNLSYSVFDKGVE*EARSALNCNNHVAM*IAWDSLCHWMTYFQFEWLCLMNASLLL 1148
            L  ID  SY       + EA ++LNCN+ V   +  DSL +W+T F  +  C +NAS LL
Sbjct: 468  LQGIDISSYYHASTTGDLEAGNSLNCNHPVVQQMVLDSLRYWVTEFHIDGFCFINASSLL 527

Query: 1149 GGSNN---------EDVPFD*VNLISDTKIFADCRDPCVMSPKEICFPHLKRWLT*IHDL 1301
             G            E + FD   L++ TK  ADC DP  M PKE  FPH KRW       
Sbjct: 528  HGVKGEYQSRPPLVEAITFD--PLLAKTKFIADCWDPHEMVPKETRFPHWKRWAEVNTRF 585

Query: 1302 VRMKEN-LEG-GVYSS*TKMACGNGYVFLGGWPHNFFYFYCKELWTTP-------*GLGL 1454
                 N L G G+ S      CGNG +F  G    F + +       P        G  L
Sbjct: 586  CNDVRNFLRGEGLLSDLATRLCGNGDIFSDGRGPAFSFNFITRNSGLPLVDLVSFSGSEL 645

Query: 1455 LS*QCTLSNQVGPANKTGVLE 1517
             S       + GP +KT VLE
Sbjct: 646  ASELSWNCGEEGPTSKTAVLE 666



 Score = 58.9 bits (141), Expect(2) = 8e-24
 Identities = 40/99 (40%), Positives = 50/99 (50%), Gaps = 15/99 (15%)
 Frame = +2

Query: 680 FTKDKSSQPPTDVVGTLFWFGNF--HHLKXXXXXXXXXXXFPFMKKKKRGPYFSYHFLS- 850
           FT+ KSS+ PT+V GT         H              FPF ++K  GPYF  HF S 
Sbjct: 365 FTEHKSSKLPTNVAGTFSGLTQKLDHFQDLGVNAILLEPIFPFDEEK--GPYFPCHFFSP 422

Query: 851 ------------AI*SMKEMIKDFHAYNVEVLLEVGFTH 931
                       +I SMKEM+K+FHA  +EV+LEV FTH
Sbjct: 423 MNCFGPSGGPIASINSMKEMVKEFHANGIEVILEVIFTH 461


>ref|XP_006418247.1| hypothetical protein EUTSA_v10006762mg [Eutrema salsugineum]
            gi|557096018|gb|ESQ36600.1| hypothetical protein
            EUTSA_v10006762mg [Eutrema salsugineum]
          Length = 878

 Score = 76.6 bits (187), Expect(3) = 1e-23
 Identities = 54/162 (33%), Positives = 78/162 (48%), Gaps = 13/162 (8%)
 Frame = +3

Query: 969  LYAIDNLSYSVFDKGVE*EARSALNCNNHVAM*IAWDSLCHWMTYFQFEWLCLMNASLLL 1148
            L  ID+  Y    +  + +++S LNCN  V   +  +SL +W+T F  +  C +NAS LL
Sbjct: 471  LRGIDDSCYYYKGRANDLDSKSYLNCNYPVVQQLILESLRYWVTEFHVDGFCFINASSLL 530

Query: 1149 GGSNNED---------VPFD*VNLISDTKIFADCRDP--CVMSPKEICFPHLKRWLT*IH 1295
             G + E          + FD   L+++TK+ ADC DP   ++ PKE+ FPH KRW     
Sbjct: 531  RGVHGEHLSRPPLVEAIAFD--PLLAETKLIADCWDPHDSMVIPKEVRFPHWKRWAELNT 588

Query: 1296 DLVRMKENL--EGGVYSS*TKMACGNGYVFLGGWPHNFFYFY 1415
               R   N     GV S      CG+G +F  G    F + Y
Sbjct: 589  RYCRNVRNFVRGRGVLSDLATRICGSGDIFTDGRGPAFSFNY 630



 Score = 55.5 bits (132), Expect(3) = 1e-23
 Identities = 37/103 (35%), Positives = 51/103 (49%), Gaps = 14/103 (13%)
 Frame = +2

Query: 665 LIVECFTKDKSSQPPTDVVGTLFWFGN-FHHLKXXXXXXXXXXXFPFMKKKKRGPYFSYH 841
           L V+ FT+ KS + P++V GT        +HLK             F   ++ GPYF +H
Sbjct: 363 LNVKGFTQHKSCKLPSNVAGTFSGVAEKVNHLKTLGTNAVLLEPI-FSFSEQEGPYFPFH 421

Query: 842 FLS-------------AI*SMKEMIKDFHAYNVEVLLEVGFTH 931
           F S             A+ SMKEM+K  H+  +EV+LEV FTH
Sbjct: 422 FFSPMDMYGPSNGHESAVNSMKEMVKKLHSQGIEVILEVVFTH 464



 Score = 26.6 bits (57), Expect(3) = 1e-23
 Identities = 14/29 (48%), Positives = 17/29 (58%)
 Frame = +1

Query: 1390 GLTISFIFIARNFGLLLEDLVCFRNNALS 1476
            G   SF +I+RN GL L DLV F    L+
Sbjct: 623  GPAFSFNYISRNSGLSLVDLVSFSGPELA 651


>gb|EYU33286.1| hypothetical protein MIMGU_mgv1a001869mg [Mimulus guttatus]
          Length = 747

 Score = 80.9 bits (198), Expect(2) = 3e-23
 Identities = 75/231 (32%), Positives = 109/231 (47%), Gaps = 17/231 (7%)
 Frame = +3

Query: 969  LYAIDNLSYSVFDKGVE*EARSALNCNNHVAM*IAWDSLCHWMTYFQFEWLCLMNASLLL 1148
            L  IDN SY   + G E ++R++LNCN  +   +  + L +W+T F  +  C +N+S L 
Sbjct: 365  LREIDNSSYHHVEGGEELKSRNSLNCNYPIVQQMILECLRYWVTEFHIDGFCFINSSSLT 424

Query: 1149 GGSNN---------EDVPFD*VNLISDTKIFADCRDPCVMSPKEICFPHLKRWLT*IHDL 1301
             G +          E + FD   L+S  KI AD  DP  M  KEI FPH KRW       
Sbjct: 425  RGFHGEFLSRPPLVEAIAFD--PLLSKVKIVADSWDPHEMEMKEIVFPHWKRWAEMNAKF 482

Query: 1302 VRMKENL---EGGVYSS*TKMACGNGYVFLGGWPHNF-FYFYCKELWTTP*GLGLLS*QC 1469
                 N    +G + S  T++ CG+G +FLGG    F F F  + +  T   + L+S   
Sbjct: 483  CSEMRNFFRGKGSISSLATRL-CGSGDLFLGGRSPAFSFNFVTRNVGPTL--VDLVSYNS 539

Query: 1470 TLSN----QVGPANKTGVLEGWLNTATKMEDLLSKYLVEMVANLVSNLLMG 1610
              S+    + GP NK  VLE      T+++ + +   V  V+  V  L MG
Sbjct: 540  IESSWNCGEEGPTNKILVLE------TRLKQIRNFIFVLFVSLGVPVLNMG 584



 Score = 56.6 bits (135), Expect(2) = 3e-23
 Identities = 40/104 (38%), Positives = 51/104 (49%), Gaps = 15/104 (14%)
 Frame = +2

Query: 665 LIVECFTKDKSSQPPTDVVGTLFWFGN-FHHLKXXXXXXXXXXX-FPFMKKKKRGPYFSY 838
           L V  FT DKSS+ P D+ G+     +  H+ K            FPF ++K  GPYF +
Sbjct: 257 LNVARFTNDKSSKLPRDIAGSFSGISDKLHYFKNLGVNAVLLEPIFPFDEQK--GPYFPW 314

Query: 839 HFLS-------------AI*SMKEMIKDFHAYNVEVLLEVGFTH 931
           HF S                S+KEM+K  HA  +EVLLEV FTH
Sbjct: 315 HFFSPGSLYGPSGDPSKVANSLKEMVKKLHANGMEVLLEVVFTH 358


>ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|223527143|gb|EEF29318.1|
            isoamylase, putative [Ricinus communis]
          Length = 872

 Score = 77.8 bits (190), Expect(2) = 7e-23
 Identities = 67/202 (33%), Positives = 90/202 (44%), Gaps = 19/202 (9%)
 Frame = +3

Query: 969  LYAIDNLSYSVFDKGVE*EARSALNCNNHVAM*IAWDSLCHWMTYFQFEWLCLMNASLLL 1148
            L  ID+ SY      ++  +R+ALNCN  +   +  DSL HW+T F  +  C +NAS LL
Sbjct: 474  LQGIDDFSYYYTKSSMD--SRNALNCNYPIVQRMILDSLQHWVTEFHIDGFCFINASALL 531

Query: 1149 GGSNNED---------VPFD*VNLISDTKIFADCRDPCVMSPKEICFPHLKRWLT*IHD- 1298
             G + E          + FD   ++S TKI AD   P    PKE CFPH KRW   I+  
Sbjct: 532  TGFHGEHLSRPPLVEAIAFD--PILSKTKIIADPWHPEHRIPKETCFPHWKRWAE-INPK 588

Query: 1299 -LVRMKENLEG-GVYSS*TKMACGNGYVFLGGWPHNFFYFYCKELWTTP-------*GLG 1451
              + ++  L G  +        CG+G +F  G    F + Y       P        G  
Sbjct: 589  FCIDVRNFLRGESLLGDLATRLCGSGDIFSNGRGPAFSFNYIARNSGLPLVDLVSFSGGE 648

Query: 1452 LLS*QCTLSNQVGPANKTGVLE 1517
            L S       + GP NKT VLE
Sbjct: 649  LGSELSWNCGEEGPTNKTAVLE 670



 Score = 58.5 bits (140), Expect(2) = 7e-23
 Identities = 41/104 (39%), Positives = 52/104 (50%), Gaps = 15/104 (14%)
 Frame = +2

Query: 665 LIVECFTKDKSSQPPTDVVGTLFWFGNF--HHLKXXXXXXXXXXXFPFMKKKKRGPYFSY 838
           L V+ FT+ KS +  +D+ GT         H              FPF ++K  GPYF Y
Sbjct: 366 LNVKRFTEHKSGKLYSDIAGTFAGLIEKMDHFRNLGVNAVLLEPIFPFDEQK--GPYFPY 423

Query: 839 HF-------------LSAI*SMKEMIKDFHAYNVEVLLEVGFTH 931
           HF             +SAI SMKEM+K+ HA  +EVLLEV FTH
Sbjct: 424 HFFSPSNIYGPSGGSISAITSMKEMVKELHANRIEVLLEVVFTH 467


>ref|XP_002441051.1| hypothetical protein SORBIDRAFT_09g019480 [Sorghum bicolor]
            gi|241946336|gb|EES19481.1| hypothetical protein
            SORBIDRAFT_09g019480 [Sorghum bicolor]
          Length = 800

 Score = 65.9 bits (159), Expect(4) = 1e-22
 Identities = 45/125 (36%), Positives = 58/125 (46%), Gaps = 9/125 (7%)
 Frame = +3

Query: 933  TDDGDCALSNIGLYAIDNLSYSVFDKGVE*EARSALNCNNHVAM*IAWDSLCHWMTYFQF 1112
            T +G      I L  ID  SY + D G+     S LNCN+ V   +  DSL HW+  F  
Sbjct: 394  TAEGGAECQMISLRGIDGSSYYIAD-GIAGCKASVLNCNHPVTQKLILDSLRHWVLDFHV 452

Query: 1113 EWLCLMNASLLLGGSNNED---------VPFD*VNLISDTKIFADCRDPCVMSPKEICFP 1265
            +  C +NA  L+ G   E          + FD V  +S TKI AD   P  +S  +  FP
Sbjct: 453  DGFCFINAPFLVRGPGGEGLSRPPLLEAIAFDPV--LSKTKIIADPWSPLDISNVQFPFP 510

Query: 1266 HLKRW 1280
            H KRW
Sbjct: 511  HWKRW 515



 Score = 59.3 bits (142), Expect(4) = 1e-22
 Identities = 43/104 (41%), Positives = 50/104 (48%), Gaps = 17/104 (16%)
 Frame = +2

Query: 671 VECFTKDKSSQPPTDVVGTLFWFG----NFHHLKXXXXXXXXXXXFPFMKKKKRGPYFSY 838
           V  FTKDKSS    DV GT         +F HL            FP+ + K  GPYF Y
Sbjct: 294 VALFTKDKSSGLADDVAGTFSGMAAKVKHFRHL--GVNAVLLEPVFPYHQVK--GPYFPY 349

Query: 839 HF-------------LSAI*SMKEMIKDFHAYNVEVLLEVGFTH 931
           HF             +SAI SMK+M+K  H   +EVLLEV FTH
Sbjct: 350 HFFSPMSLYSSECSSVSAIKSMKDMVKTMHRNGIEVLLEVVFTH 393



 Score = 28.9 bits (63), Expect(4) = 1e-22
 Identities = 16/39 (41%), Positives = 21/39 (53%)
 Frame = +1

Query: 1357 LVGMGMFSWVAGLTISFIFIARNFGLLLEDLVCFRNNAL 1473
            L G G      G   SF +++RN GL L DLV F ++ L
Sbjct: 543  LCGSGDLFSSRGPAFSFNYVSRNSGLTLVDLVSFSSDEL 581



 Score = 21.2 bits (43), Expect(4) = 1e-22
 Identities = 10/21 (47%), Positives = 12/21 (57%)
 Frame = +2

Query: 1259 FSPLEKMVNMNT*FSEDERKF 1321
            F   ++   MNT FS D RKF
Sbjct: 509  FPHWKRWAEMNTRFSMDVRKF 529


>ref|XP_006386822.1| hypothetical protein POPTR_0002s22530g [Populus trichocarpa]
            gi|550345605|gb|ERP64619.1| hypothetical protein
            POPTR_0002s22530g [Populus trichocarpa]
          Length = 857

 Score = 77.0 bits (188), Expect(2) = 3e-22
 Identities = 65/201 (32%), Positives = 89/201 (44%), Gaps = 18/201 (8%)
 Frame = +3

Query: 969  LYAIDNLSYSVFDKGVE*EARSALNCNNHVAM*IAWDSLCHWMTYFQFEWLCLMNASLLL 1148
            L  ID+ SY         ++++ALNCN+ +   +  DSL HW+T F  +  C +NA  LL
Sbjct: 462  LQGIDDSSYYCTSITTGLDSQNALNCNHPIVQRVILDSLQHWVTEFHIDGFCFINALSLL 521

Query: 1149 ---GGSN------NEDVPFD*VNLISDTKIFADCRDPCVMSPKEICFPHLKRWL-T*IHD 1298
               GG         E + FD   L S TKI ADC DP  +  KE  FPH K+W       
Sbjct: 522  RSFGGEYLSRPPVVEAIAFD--PLFSKTKIIADCWDPKELEAKETAFPHWKKWAEMNTKF 579

Query: 1299 LVRMKENLEG-GVYSS*TKMACGNGYVFLGGWPHNFFYFYCKELWTTP-------*GLGL 1454
               ++  L G G+ S      CG+G +F  G    F + +    +  P        G  L
Sbjct: 580  CYDIRNFLRGEGLLSDLATRLCGSGDIFSSGRGPAFSFNFITRNFGLPLVDLVSFSGDEL 639

Query: 1455 LS*QCTLSNQVGPANKTGVLE 1517
             S       + GP NKT +LE
Sbjct: 640  ASELSWNCGEEGPTNKTTILE 660



 Score = 57.0 bits (136), Expect(2) = 3e-22
 Identities = 41/99 (41%), Positives = 46/99 (46%), Gaps = 15/99 (15%)
 Frame = +2

Query: 680 FTKDKSSQPPTDVVGTLFWFGN--FHHLKXXXXXXXXXXXFPFMKKKKRGPYFSYHFLS- 850
           FTKD SSQ  +D  GT         H              FPF ++K  GPYF  HF S 
Sbjct: 359 FTKDNSSQISSDAAGTFAGLIEKLSHFKNLGVNAVLLESIFPFDEQK--GPYFPCHFFSP 416

Query: 851 ------------AI*SMKEMIKDFHAYNVEVLLEVGFTH 931
                       AI SMKEM+K  HA  +EVLLEV FTH
Sbjct: 417 SNVYGPSNGSVAAISSMKEMVKRLHANGIEVLLEVVFTH 455


>ref|NP_001105666.1| isoamylase-type starch debranching enzyme ISO2 [Zea mays]
            gi|29126647|gb|AAO17048.3| isoamylase-type starch
            debranching enzyme ISO2 [Zea mays]
          Length = 799

 Score = 65.9 bits (159), Expect(4) = 4e-22
 Identities = 45/125 (36%), Positives = 58/125 (46%), Gaps = 9/125 (7%)
 Frame = +3

Query: 933  TDDGDCALSNIGLYAIDNLSYSVFDKGVE*EARSALNCNNHVAM*IAWDSLCHWMTYFQF 1112
            T +G      I L  ID  SY + D G+     S LNCN+ V   +  DSL HW+  F  
Sbjct: 393  TAEGGAECQMISLRGIDGSSYYIAD-GIAGCKASVLNCNHPVTQKLILDSLRHWVLDFHV 451

Query: 1113 EWLCLMNASLLLGGSNNED---------VPFD*VNLISDTKIFADCRDPCVMSPKEICFP 1265
            +  C +NA  L+ G   E          + FD V  +S TKI AD   P  +S  +  FP
Sbjct: 452  DGFCFINAPFLVRGPGGEGLSRPPLLEAIAFDPV--LSKTKIIADPWSPLDISNVQFPFP 509

Query: 1266 HLKRW 1280
            H KRW
Sbjct: 510  HWKRW 514



 Score = 57.0 bits (136), Expect(4) = 4e-22
 Identities = 41/104 (39%), Positives = 50/104 (48%), Gaps = 17/104 (16%)
 Frame = +2

Query: 671 VECFTKDKSSQPPTDVVGTLFWFG----NFHHLKXXXXXXXXXXXFPFMKKKKRGPYFSY 838
           V  FTKD+SS+   +  GT         +F HL            FPF + K  GPYF Y
Sbjct: 293 VALFTKDRSSRLADNAAGTFSGMSAKVEHFRHL--GVNAVLLEPVFPFHQVK--GPYFPY 348

Query: 839 HF-------------LSAI*SMKEMIKDFHAYNVEVLLEVGFTH 931
           HF             +SAI SMK+M+K  H   +EVLLEV FTH
Sbjct: 349 HFFSPMSLYSSECSSVSAIKSMKDMVKTMHRNGIEVLLEVVFTH 392



 Score = 29.3 bits (64), Expect(4) = 4e-22
 Identities = 16/40 (40%), Positives = 22/40 (55%)
 Frame = +1

Query: 1357 LVGMGMFSWVAGLTISFIFIARNFGLLLEDLVCFRNNALS 1476
            L G G      G   SF +++RN GL L DLV F ++ L+
Sbjct: 542  LCGSGDLFSSRGPAFSFNYVSRNSGLTLVDLVSFSSDELA 581



 Score = 21.2 bits (43), Expect(4) = 4e-22
 Identities = 10/21 (47%), Positives = 12/21 (57%)
 Frame = +2

Query: 1259 FSPLEKMVNMNT*FSEDERKF 1321
            F   ++   MNT FS D RKF
Sbjct: 508  FPHWKRWAEMNTRFSMDVRKF 528


>gb|ACG48178.1| isoamylase-type starch debranching enzyme ISO2 [Zea mays]
          Length = 799

 Score = 65.9 bits (159), Expect(4) = 4e-22
 Identities = 45/125 (36%), Positives = 58/125 (46%), Gaps = 9/125 (7%)
 Frame = +3

Query: 933  TDDGDCALSNIGLYAIDNLSYSVFDKGVE*EARSALNCNNHVAM*IAWDSLCHWMTYFQF 1112
            T +G      I L  ID  SY + D G+     S LNCN+ V   +  DSL HW+  F  
Sbjct: 393  TAEGGAECQMISLRGIDGSSYYIAD-GIAGCKASVLNCNHPVTQRLILDSLRHWVLDFHV 451

Query: 1113 EWLCLMNASLLLGGSNNED---------VPFD*VNLISDTKIFADCRDPCVMSPKEICFP 1265
            +  C +NA  L+ G   E          + FD V  +S TKI AD   P  +S  +  FP
Sbjct: 452  DGFCFINAPFLVRGPGGEGLSRPPLLEAIAFDPV--LSKTKIIADPWSPLDISNVQFPFP 509

Query: 1266 HLKRW 1280
            H KRW
Sbjct: 510  HWKRW 514



 Score = 57.0 bits (136), Expect(4) = 4e-22
 Identities = 41/104 (39%), Positives = 50/104 (48%), Gaps = 17/104 (16%)
 Frame = +2

Query: 671 VECFTKDKSSQPPTDVVGTLFWFG----NFHHLKXXXXXXXXXXXFPFMKKKKRGPYFSY 838
           V  FTKD+SS+   +  GT         +F HL            FPF + K  GPYF Y
Sbjct: 293 VALFTKDRSSRLADNAAGTFSGMSAKVEHFRHL--GVNAVLLEPVFPFHQVK--GPYFPY 348

Query: 839 HF-------------LSAI*SMKEMIKDFHAYNVEVLLEVGFTH 931
           HF             +SAI SMK+M+K  H   +EVLLEV FTH
Sbjct: 349 HFFSPMSLYSSECSSVSAIKSMKDMVKTMHRNGIEVLLEVVFTH 392



 Score = 29.3 bits (64), Expect(4) = 4e-22
 Identities = 16/40 (40%), Positives = 22/40 (55%)
 Frame = +1

Query: 1357 LVGMGMFSWVAGLTISFIFIARNFGLLLEDLVCFRNNALS 1476
            L G G      G   SF +++RN GL L DLV F ++ L+
Sbjct: 542  LCGSGDLFSSRGPAFSFNYVSRNSGLTLVDLVSFSSDELA 581



 Score = 21.2 bits (43), Expect(4) = 4e-22
 Identities = 10/21 (47%), Positives = 12/21 (57%)
 Frame = +2

Query: 1259 FSPLEKMVNMNT*FSEDERKF 1321
            F   ++   MNT FS D RKF
Sbjct: 508  FPHWKRWAEMNTRFSMDVRKF 528


>gb|EAY96723.1| hypothetical protein OsI_18641 [Oryza sativa Indica Group]
          Length = 840

 Score = 65.5 bits (158), Expect(3) = 1e-21
 Identities = 44/125 (35%), Positives = 60/125 (48%), Gaps = 9/125 (7%)
 Frame = +3

Query: 933  TDDGDCALSNIGLYAIDNLSYSVFDKGVE*EARSALNCNNHVAM*IAWDSLCHWMTYFQF 1112
            T +G+     I +  IDN SY + + G+     S LNCN+ V   +  DSL HW+  F  
Sbjct: 434  TAEGESECQTISMRGIDNSSYYIAN-GIAGCKASILNCNHPVTQKLILDSLRHWVLDFHV 492

Query: 1113 EWLCLMNASLLLGGSNN---------EDVPFD*VNLISDTKIFADCRDPCVMSPKEICFP 1265
            +  C +NA  L+ G            E + FD V  +S TKI AD   P  +S  +  FP
Sbjct: 493  DGFCFINAPFLVRGPGGEYLSRPPLLEAIAFDPV--LSMTKIIADPWSPLDISNVQFPFP 550

Query: 1266 HLKRW 1280
            H KRW
Sbjct: 551  HWKRW 555



 Score = 57.0 bits (136), Expect(3) = 1e-21
 Identities = 40/102 (39%), Positives = 47/102 (46%), Gaps = 15/102 (14%)
 Frame = +2

Query: 671 VECFTKDKSSQPPTDVVGTLFWFGNF--HHLKXXXXXXXXXXXFPFMKKKKRGPYFSYHF 844
           V  FTKD+SS  P D  GT         H              FPF + K  GPYF YHF
Sbjct: 334 VALFTKDRSSGLPDDAAGTFTGLSAKVEHFRSLGVNAILLEPVFPFHQVK--GPYFPYHF 391

Query: 845 -------------LSAI*SMKEMIKDFHAYNVEVLLEVGFTH 931
                        +SAI SMK+M++  H   +EVLLEV FTH
Sbjct: 392 FSPMNLYSSKGLSVSAIKSMKDMVRVMHRNGIEVLLEVVFTH 433



 Score = 29.3 bits (64), Expect(3) = 1e-21
 Identities = 17/39 (43%), Positives = 20/39 (51%)
 Frame = +1

Query: 1357 LVGMGMFSWVAGLTISFIFIARNFGLLLEDLVCFRNNAL 1473
            L G G      G   SF  ++RN GL L DLV F N+ L
Sbjct: 583  LCGSGDLFSTRGPAFSFNHVSRNSGLSLVDLVSFSNDDL 621