BLASTX nr result
ID: Akebia24_contig00048605
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Akebia24_contig00048605
(342 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_006472234.1| PREDICTED: pentatricopeptide repeat-containi... 156 2e-36
ref|XP_006433563.1| hypothetical protein CICLE_v10000386mg [Citr... 156 2e-36
ref|XP_007207552.1| hypothetical protein PRUPE_ppa017672mg [Prun... 151 8e-35
ref|XP_006382364.1| hypothetical protein POPTR_0005s01450g, part... 150 1e-34
emb|CBI39619.3| unnamed protein product [Vitis vinifera] 148 9e-34
ref|XP_002277347.1| PREDICTED: pentatricopeptide repeat-containi... 148 9e-34
ref|XP_004304908.1| PREDICTED: pentatricopeptide repeat-containi... 143 3e-32
ref|XP_004500093.1| PREDICTED: pentatricopeptide repeat-containi... 142 5e-32
ref|XP_007137380.1| hypothetical protein PHAVU_009G122500g [Phas... 142 6e-32
ref|XP_006344853.1| PREDICTED: pentatricopeptide repeat-containi... 141 8e-32
gb|EXC31178.1| hypothetical protein L484_004944 [Morus notabilis] 140 1e-31
gb|EYU44038.1| hypothetical protein MIMGU_mgv1a001643mg [Mimulus... 140 2e-31
ref|XP_006602194.1| PREDICTED: pentatricopeptide repeat-containi... 139 4e-31
ref|XP_007029178.1| Pentatricopeptide repeat (PPR) superfamily p... 130 2e-28
ref|XP_002322762.2| hypothetical protein POPTR_0016s06560g [Popu... 128 7e-28
ref|XP_006483347.1| PREDICTED: pentatricopeptide repeat-containi... 128 9e-28
ref|XP_006483346.1| PREDICTED: pentatricopeptide repeat-containi... 128 9e-28
ref|XP_006450458.1| hypothetical protein CICLE_v10010438mg, part... 128 9e-28
ref|XP_003530680.1| PREDICTED: putative pentatricopeptide repeat... 127 1e-27
gb|EMT31594.1| hypothetical protein F775_06879 [Aegilops tauschii] 127 2e-27
>ref|XP_006472234.1| PREDICTED: pentatricopeptide repeat-containing protein At3g22690-like
[Citrus sinensis]
Length = 798
Score = 156 bits (395), Expect = 2e-36
Identities = 74/109 (67%), Positives = 83/109 (76%)
Frame = -3
Query: 340 EIERMLDEMAKRLKMDGYAPDTNDVAFDIDDEEKETSLHRHSEKXXXXXXXXXXXXXXXX 161
EI+ MLDEMAK+LK++GYAPDT +VAFDID EEKET+L RHSEK
Sbjct: 690 EIDNMLDEMAKKLKLEGYAPDTLEVAFDIDQEEKETTLFRHSEKLAIAFGLITISPPNPI 749
Query: 160 XILKNLRICGDCHNMAKAISRIFEREIVVRDRHRFHHFKHGSCSCMDYW 14
I+KNLRIC DCH AK ISR F+REIVVRDRHRFHHFKHGSCSCMD+W
Sbjct: 750 RIMKNLRICNDCHTAAKFISRAFDREIVVRDRHRFHHFKHGSCSCMDFW 798
>ref|XP_006433563.1| hypothetical protein CICLE_v10000386mg [Citrus clementina]
gi|557535685|gb|ESR46803.1| hypothetical protein
CICLE_v10000386mg [Citrus clementina]
Length = 746
Score = 156 bits (395), Expect = 2e-36
Identities = 74/109 (67%), Positives = 83/109 (76%)
Frame = -3
Query: 340 EIERMLDEMAKRLKMDGYAPDTNDVAFDIDDEEKETSLHRHSEKXXXXXXXXXXXXXXXX 161
EI+ MLDEMAK+LK++GYAPDT +VAFDID EEKET+L RHSEK
Sbjct: 638 EIDNMLDEMAKKLKLEGYAPDTLEVAFDIDQEEKETTLFRHSEKLAIAFGLITISPPNPI 697
Query: 160 XILKNLRICGDCHNMAKAISRIFEREIVVRDRHRFHHFKHGSCSCMDYW 14
I+KNLRIC DCH AK ISR F+REIVVRDRHRFHHFKHGSCSCMD+W
Sbjct: 698 RIMKNLRICNDCHTAAKFISRAFDREIVVRDRHRFHHFKHGSCSCMDFW 746
>ref|XP_007207552.1| hypothetical protein PRUPE_ppa017672mg [Prunus persica]
gi|462403194|gb|EMJ08751.1| hypothetical protein
PRUPE_ppa017672mg [Prunus persica]
Length = 745
Score = 151 bits (382), Expect = 8e-35
Identities = 71/109 (65%), Positives = 82/109 (75%)
Frame = -3
Query: 340 EIERMLDEMAKRLKMDGYAPDTNDVAFDIDDEEKETSLHRHSEKXXXXXXXXXXXXXXXX 161
EIE+ LDEMAK+LKM+GYAPDTN+V+FDID+EEKET+L RHSEK
Sbjct: 637 EIEKKLDEMAKKLKMEGYAPDTNEVSFDIDEEEKETALFRHSEKLAIAFGLICTSPPTPI 696
Query: 160 XILKNLRICGDCHNMAKAISRIFEREIVVRDRHRFHHFKHGSCSCMDYW 14
I+KNLRIC DCH AK IS+ F R+IV+RDRHRFHHFK GSCSC DYW
Sbjct: 697 RIIKNLRICNDCHMAAKFISKAFNRDIVLRDRHRFHHFKQGSCSCKDYW 745
>ref|XP_006382364.1| hypothetical protein POPTR_0005s01450g, partial [Populus trichocarpa]
gi|550337723|gb|ERP60161.1| hypothetical protein
POPTR_0005s01450g, partial [Populus trichocarpa]
Length = 788
Score = 150 bits (380), Expect = 1e-34
Identities = 71/108 (65%), Positives = 80/108 (74%)
Frame = -3
Query: 337 IERMLDEMAKRLKMDGYAPDTNDVAFDIDDEEKETSLHRHSEKXXXXXXXXXXXXXXXXX 158
IE MLDEMAK+LK++GYAPDT +V+ DID+EEKET+L RHSEK
Sbjct: 681 IEHMLDEMAKKLKLEGYAPDTREVSLDIDEEEKETTLFRHSEKLAIAFGLIAIDPPTPIR 740
Query: 157 ILKNLRICGDCHNMAKAISRIFEREIVVRDRHRFHHFKHGSCSCMDYW 14
I+KNLRIC DCH AK IS+ F REIVVRDRHRFHHFK GSCSCMDYW
Sbjct: 741 IVKNLRICNDCHTAAKLISKAFNREIVVRDRHRFHHFKQGSCSCMDYW 788
>emb|CBI39619.3| unnamed protein product [Vitis vinifera]
Length = 640
Score = 148 bits (373), Expect = 9e-34
Identities = 69/109 (63%), Positives = 82/109 (75%)
Frame = -3
Query: 340 EIERMLDEMAKRLKMDGYAPDTNDVAFDIDDEEKETSLHRHSEKXXXXXXXXXXXXXXXX 161
++E ML+EMAKRLKM+GYAPDTN+V+ DID+EEKET+L RHSEK
Sbjct: 532 KVEGMLNEMAKRLKMEGYAPDTNEVSLDIDEEEKETTLFRHSEKLAIAFGLLTISPPTPI 591
Query: 160 XILKNLRICGDCHNMAKAISRIFEREIVVRDRHRFHHFKHGSCSCMDYW 14
I+KNLRIC DCH AK IS+ + REIVVRDRHRFH+FK G+CSCMDYW
Sbjct: 592 RIMKNLRICNDCHTAAKLISKAYAREIVVRDRHRFHYFKEGACSCMDYW 640
>ref|XP_002277347.1| PREDICTED: pentatricopeptide repeat-containing protein At1g08070-like
[Vitis vinifera]
Length = 808
Score = 148 bits (373), Expect = 9e-34
Identities = 69/109 (63%), Positives = 82/109 (75%)
Frame = -3
Query: 340 EIERMLDEMAKRLKMDGYAPDTNDVAFDIDDEEKETSLHRHSEKXXXXXXXXXXXXXXXX 161
++E ML+EMAKRLKM+GYAPDTN+V+ DID+EEKET+L RHSEK
Sbjct: 700 KVEGMLNEMAKRLKMEGYAPDTNEVSLDIDEEEKETTLFRHSEKLAIAFGLLTISPPTPI 759
Query: 160 XILKNLRICGDCHNMAKAISRIFEREIVVRDRHRFHHFKHGSCSCMDYW 14
I+KNLRIC DCH AK IS+ + REIVVRDRHRFH+FK G+CSCMDYW
Sbjct: 760 RIMKNLRICNDCHTAAKLISKAYAREIVVRDRHRFHYFKEGACSCMDYW 808
>ref|XP_004304908.1| PREDICTED: pentatricopeptide repeat-containing protein
At1g08070-like [Fragaria vesca subsp. vesca]
Length = 777
Score = 143 bits (360), Expect = 3e-32
Identities = 68/109 (62%), Positives = 80/109 (73%)
Frame = -3
Query: 340 EIERMLDEMAKRLKMDGYAPDTNDVAFDIDDEEKETSLHRHSEKXXXXXXXXXXXXXXXX 161
EIE ML EMA++LK +GYAPDTN+V+FDID+EEKET+L RHSEK
Sbjct: 669 EIEVMLYEMAQKLKREGYAPDTNEVSFDIDEEEKETALFRHSEKLAIAFGLIAISPPSPI 728
Query: 160 XILKNLRICGDCHNMAKAISRIFEREIVVRDRHRFHHFKHGSCSCMDYW 14
I+KNLRIC DCH AK +S+ F R+IVVRD+HRFHHFK GSCSC DYW
Sbjct: 729 RIIKNLRICNDCHMAAKFVSKAFGRDIVVRDKHRFHHFKQGSCSCNDYW 777
>ref|XP_004500093.1| PREDICTED: pentatricopeptide repeat-containing protein At2g29760,
chloroplastic-like isoform X1 [Cicer arietinum]
gi|502128825|ref|XP_004500094.1| PREDICTED:
pentatricopeptide repeat-containing protein At2g29760,
chloroplastic-like isoform X2 [Cicer arietinum]
Length = 785
Score = 142 bits (358), Expect = 5e-32
Identities = 65/109 (59%), Positives = 80/109 (73%)
Frame = -3
Query: 340 EIERMLDEMAKRLKMDGYAPDTNDVAFDIDDEEKETSLHRHSEKXXXXXXXXXXXXXXXX 161
+IE MLD MA +LK++GYAP T++V+ DID+EEKET+L HSEK
Sbjct: 677 DIEHMLDVMAAKLKIEGYAPITSEVSLDIDEEEKETALFSHSEKLAVAFGLITIAPPAPI 736
Query: 160 XILKNLRICGDCHNMAKAISRIFEREIVVRDRHRFHHFKHGSCSCMDYW 14
I+KNLRIC DCH + K IS+ F+REIV+RDRHRFHHFKHGSCSCMD+W
Sbjct: 737 RIMKNLRICNDCHTVVKLISKAFDREIVIRDRHRFHHFKHGSCSCMDFW 785
>ref|XP_007137380.1| hypothetical protein PHAVU_009G122500g [Phaseolus vulgaris]
gi|561010467|gb|ESW09374.1| hypothetical protein
PHAVU_009G122500g [Phaseolus vulgaris]
Length = 774
Score = 142 bits (357), Expect = 6e-32
Identities = 63/109 (57%), Positives = 80/109 (73%)
Frame = -3
Query: 340 EIERMLDEMAKRLKMDGYAPDTNDVAFDIDDEEKETSLHRHSEKXXXXXXXXXXXXXXXX 161
+IE MLD +A +LK++GY P T++V+ DIDDEEKET+L RHSEK
Sbjct: 666 DIEHMLDVVAAKLKIEGYVPTTSEVSLDIDDEEKETALFRHSEKLAVAFGLITIAPPTPI 725
Query: 160 XILKNLRICGDCHNMAKAISRIFEREIVVRDRHRFHHFKHGSCSCMDYW 14
++KNLRIC DCH + K IS+ F+REIVVRDRHRFHHF+HG+CSCMD+W
Sbjct: 726 RVMKNLRICNDCHTVVKLISKAFDREIVVRDRHRFHHFRHGACSCMDFW 774
>ref|XP_006344853.1| PREDICTED: pentatricopeptide repeat-containing protein
At1g08070-like [Solanum tuberosum]
Length = 745
Score = 141 bits (356), Expect = 8e-32
Identities = 68/109 (62%), Positives = 79/109 (72%)
Frame = -3
Query: 340 EIERMLDEMAKRLKMDGYAPDTNDVAFDIDDEEKETSLHRHSEKXXXXXXXXXXXXXXXX 161
EIE ML EM KRLK+ GYAPDT++V DID+EEKE++L RHSEK
Sbjct: 637 EIEEMLAEMEKRLKIMGYAPDTDEVLLDIDEEEKESTLFRHSEKLAIAYGLIAIAPPTPI 696
Query: 160 XILKNLRICGDCHNMAKAISRIFEREIVVRDRHRFHHFKHGSCSCMDYW 14
I+KNLRIC DCH AK IS+ F+REIVVRDRHRFHHFK GSCSCM++W
Sbjct: 697 RIIKNLRICSDCHAAAKLISKAFDREIVVRDRHRFHHFKDGSCSCMEFW 745
>gb|EXC31178.1| hypothetical protein L484_004944 [Morus notabilis]
Length = 745
Score = 140 bits (354), Expect = 1e-31
Identities = 67/109 (61%), Positives = 78/109 (71%)
Frame = -3
Query: 340 EIERMLDEMAKRLKMDGYAPDTNDVAFDIDDEEKETSLHRHSEKXXXXXXXXXXXXXXXX 161
EIE LDE+A +LKM+GYAPDTN+V DID EEKET+L +HSEK
Sbjct: 637 EIEAKLDEIAIKLKMEGYAPDTNEVPLDIDKEEKETALLKHSEKLAIAFGLISTSPPTPI 696
Query: 160 XILKNLRICGDCHNMAKAISRIFEREIVVRDRHRFHHFKHGSCSCMDYW 14
I+KNLRIC DCHN AK IS+ ++R IVVRDRHRFHHF GSCSC+DYW
Sbjct: 697 RIMKNLRICNDCHNAAKFISKAYDRVIVVRDRHRFHHFDQGSCSCLDYW 745
>gb|EYU44038.1| hypothetical protein MIMGU_mgv1a001643mg [Mimulus guttatus]
Length = 780
Score = 140 bits (352), Expect = 2e-31
Identities = 66/109 (60%), Positives = 75/109 (68%)
Frame = -3
Query: 340 EIERMLDEMAKRLKMDGYAPDTNDVAFDIDDEEKETSLHRHSEKXXXXXXXXXXXXXXXX 161
EI+ ML EM +RLK GY P +V DID+EEKET+L RHSEK
Sbjct: 672 EIDGMLAEMGRRLKRLGYTPGVGEVLLDIDEEEKETNLSRHSEKLAIAFGLFATSGPAPI 731
Query: 160 XILKNLRICGDCHNMAKAISRIFEREIVVRDRHRFHHFKHGSCSCMDYW 14
I+KNLRICGDCH AK +S F+REIV+RDRHRFHHFKHGSCSCMDYW
Sbjct: 732 RIIKNLRICGDCHAAAKLVSEAFDREIVIRDRHRFHHFKHGSCSCMDYW 780
>ref|XP_006602194.1| PREDICTED: pentatricopeptide repeat-containing protein
At1g08070-like isoform X1 [Glycine max]
Length = 775
Score = 139 bits (350), Expect = 4e-31
Identities = 62/109 (56%), Positives = 79/109 (72%)
Frame = -3
Query: 340 EIERMLDEMAKRLKMDGYAPDTNDVAFDIDDEEKETSLHRHSEKXXXXXXXXXXXXXXXX 161
+IE MLD +A +LK++GY P T++V+ DID+EEKET+L RHSEK
Sbjct: 667 DIEHMLDVVAAKLKIEGYVPTTSEVSLDIDEEEKETALFRHSEKLAVAFGLITISPPTPI 726
Query: 160 XILKNLRICGDCHNMAKAISRIFEREIVVRDRHRFHHFKHGSCSCMDYW 14
+ KNLRIC DCH + K IS+ F+R+IVVRDRHRFHHFKHG+CSCMD+W
Sbjct: 727 RVTKNLRICNDCHTVVKLISKAFDRDIVVRDRHRFHHFKHGACSCMDFW 775
>ref|XP_007029178.1| Pentatricopeptide repeat (PPR) superfamily protein [Theobroma
cacao] gi|508717783|gb|EOY09680.1| Pentatricopeptide
repeat (PPR) superfamily protein [Theobroma cacao]
Length = 626
Score = 130 bits (326), Expect = 2e-28
Identities = 56/108 (51%), Positives = 78/108 (72%)
Frame = -3
Query: 337 IERMLDEMAKRLKMDGYAPDTNDVAFDIDDEEKETSLHRHSEKXXXXXXXXXXXXXXXXX 158
IER + + K++++ GY+ +T+D FDID+EEKE++L+RHSEK
Sbjct: 519 IERTWEAILKKIRLAGYSGNTSDALFDIDEEEKESALYRHSEKLAIAFGIMRTKASMPIR 578
Query: 157 ILKNLRICGDCHNMAKAISRIFEREIVVRDRHRFHHFKHGSCSCMDYW 14
I+KNLR+C DCH K IS++FERE++VRDR+RFHHF+HG+CSCMDYW
Sbjct: 579 IVKNLRVCEDCHTATKLISKVFERELIVRDRNRFHHFRHGTCSCMDYW 626
>ref|XP_002322762.2| hypothetical protein POPTR_0016s06560g [Populus trichocarpa]
gi|550320988|gb|EEF04523.2| hypothetical protein
POPTR_0016s06560g [Populus trichocarpa]
Length = 543
Score = 128 bits (322), Expect = 7e-28
Identities = 55/109 (50%), Positives = 78/109 (71%)
Frame = -3
Query: 340 EIERMLDEMAKRLKMDGYAPDTNDVAFDIDDEEKETSLHRHSEKXXXXXXXXXXXXXXXX 161
+IERM +E+ ++++ GY+ + +D FDID+EEKE+++HRHSEK
Sbjct: 435 QIERMWEEILGKIRLAGYSGNNDDALFDIDEEEKESNIHRHSEKLAIAYAIMRTKGHDPI 494
Query: 160 XILKNLRICGDCHNMAKAISRIFEREIVVRDRHRFHHFKHGSCSCMDYW 14
I+KNLR+C DCH K IS+++ERE++VRDR+RFHHFK G+CSCMDYW
Sbjct: 495 RIVKNLRVCEDCHTATKLISKVYERELIVRDRNRFHHFKGGACSCMDYW 543
>ref|XP_006483347.1| PREDICTED: pentatricopeptide repeat-containing protein At1g59720,
mitochondrial-like isoform X2 [Citrus sinensis]
Length = 566
Score = 128 bits (321), Expect = 9e-28
Identities = 58/109 (53%), Positives = 75/109 (68%)
Frame = -3
Query: 340 EIERMLDEMAKRLKMDGYAPDTNDVAFDIDDEEKETSLHRHSEKXXXXXXXXXXXXXXXX 161
EI MLDEM +++K GY P T++V DI++E+KE +L++HSEK
Sbjct: 458 EIYEMLDEMGRKMKKAGYVPTTSEVLLDINEEDKEDALNKHSEKLAIAFALLKTPPGTPI 517
Query: 160 XILKNLRICGDCHNMAKAISRIFEREIVVRDRHRFHHFKHGSCSCMDYW 14
I+KNLR+C DCH+ K IS+I+ REIVVRDRHRFHHFK GSCSC D+W
Sbjct: 518 RIVKNLRVCNDCHSATKFISKIYNREIVVRDRHRFHHFKDGSCSCRDFW 566
>ref|XP_006483346.1| PREDICTED: pentatricopeptide repeat-containing protein At1g59720,
mitochondrial-like isoform X1 [Citrus sinensis]
Length = 600
Score = 128 bits (321), Expect = 9e-28
Identities = 58/109 (53%), Positives = 75/109 (68%)
Frame = -3
Query: 340 EIERMLDEMAKRLKMDGYAPDTNDVAFDIDDEEKETSLHRHSEKXXXXXXXXXXXXXXXX 161
EI MLDEM +++K GY P T++V DI++E+KE +L++HSEK
Sbjct: 492 EIYEMLDEMGRKMKKAGYVPTTSEVLLDINEEDKEDALNKHSEKLAIAFALLKTPPGTPI 551
Query: 160 XILKNLRICGDCHNMAKAISRIFEREIVVRDRHRFHHFKHGSCSCMDYW 14
I+KNLR+C DCH+ K IS+I+ REIVVRDRHRFHHFK GSCSC D+W
Sbjct: 552 RIVKNLRVCNDCHSATKFISKIYNREIVVRDRHRFHHFKDGSCSCRDFW 600
>ref|XP_006450458.1| hypothetical protein CICLE_v10010438mg, partial [Citrus clementina]
gi|557553684|gb|ESR63698.1| hypothetical protein
CICLE_v10010438mg, partial [Citrus clementina]
Length = 629
Score = 128 bits (321), Expect = 9e-28
Identities = 58/109 (53%), Positives = 75/109 (68%)
Frame = -3
Query: 340 EIERMLDEMAKRLKMDGYAPDTNDVAFDIDDEEKETSLHRHSEKXXXXXXXXXXXXXXXX 161
EI MLDEM +++K GY P T++V DI++E+KE +L++HSEK
Sbjct: 521 EIYEMLDEMGRKMKKAGYVPTTSEVLLDINEEDKEDALNKHSEKLAIAFALLKTPPGTPI 580
Query: 160 XILKNLRICGDCHNMAKAISRIFEREIVVRDRHRFHHFKHGSCSCMDYW 14
I+KNLR+C DCH+ K IS+I+ REIVVRDRHRFHHFK GSCSC D+W
Sbjct: 581 RIVKNLRVCNDCHSATKFISKIYNREIVVRDRHRFHHFKDGSCSCRDFW 629
>ref|XP_003530680.1| PREDICTED: putative pentatricopeptide repeat-containing protein
At5g40405-like isoform X1 [Glycine max]
Length = 616
Score = 127 bits (320), Expect = 1e-27
Identities = 59/109 (54%), Positives = 75/109 (68%)
Frame = -3
Query: 340 EIERMLDEMAKRLKMDGYAPDTNDVAFDIDDEEKETSLHRHSEKXXXXXXXXXXXXXXXX 161
EIE L+E++K L++ GY +TN V FDI++EEKE +L +HSEK
Sbjct: 508 EIEMKLEEISKCLRLSGYVANTNPVLFDIEEEEKEDALSKHSEKVAIAFGLISLKGVVPI 567
Query: 160 XILKNLRICGDCHNMAKAISRIFEREIVVRDRHRFHHFKHGSCSCMDYW 14
++ NLRIC DCHN+AK IS+IF REI+VRDR+RFHHFK G CSC DYW
Sbjct: 568 RVVMNLRICWDCHNVAKMISKIFNREIIVRDRNRFHHFKDGECSCKDYW 616
>gb|EMT31594.1| hypothetical protein F775_06879 [Aegilops tauschii]
Length = 461
Score = 127 bits (319), Expect = 2e-27
Identities = 57/109 (52%), Positives = 77/109 (70%)
Frame = -3
Query: 340 EIERMLDEMAKRLKMDGYAPDTNDVAFDIDDEEKETSLHRHSEKXXXXXXXXXXXXXXXX 161
+IE ML EM+ +L++ GYA +T +V FDI++EEKE ++ HSEK
Sbjct: 353 DIEAMLAEMSHKLRLQGYAANTKEVLFDIEEEEKEGAISLHSEKLALAFGLITLPEGTVI 412
Query: 160 XILKNLRICGDCHNMAKAISRIFEREIVVRDRHRFHHFKHGSCSCMDYW 14
I+KNLR+C DCH+ K IS++F+REIV+RDR+RFHHFKHG+CSC DYW
Sbjct: 413 RIVKNLRVCKDCHDYTKMISKVFDREIVMRDRNRFHHFKHGACSCRDYW 461