BLASTX nr result
ID: Akebia24_contig00043171
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Akebia24_contig00043171
(333 letters)
Database: ./nr
38,876,450 sequences; 13,856,398,315 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CAN75186.1| hypothetical protein VITISV_001913 [Vitis vinifera] 160 2e-37
ref|XP_004149623.1| PREDICTED: uncharacterized protein LOC101211... 145 6e-33
ref|XP_004149403.1| PREDICTED: uncharacterized protein LOC101215... 141 1e-31
gb|AAB61111.1| Strong similarity to Zea mays retrotransposon Hop... 137 1e-30
dbj|BAB10503.1| retroelement pol polyprotein-like [Arabidopsis t... 134 1e-29
gb|AAC33963.1| contains similarity to reverse transcriptases (Pf... 132 7e-29
emb|CAN64225.1| hypothetical protein VITISV_016222 [Vitis vinifera] 128 9e-28
emb|CAN65540.1| hypothetical protein VITISV_029946 [Vitis vinifera] 125 5e-27
emb|CAA72989.1| unnamed protein product [Brassica oleracea var. ... 122 4e-26
gb|AAD25646.1| putative retroelement pol polyprotein [Arabidopsi... 121 9e-26
gb|AAG10817.1|AC011808_5 Putative retroelement polyprotein [Arab... 121 1e-25
emb|CAB10225.1| retrovirus-related like polyprotein [Arabidopsis... 120 3e-25
gb|AAD26943.1| putative retroelement pol polyprotein [Arabidopsi... 120 3e-25
ref|XP_004245399.1| PREDICTED: uncharacterized protein LOC101252... 119 3e-25
gb|AAF79879.1|AC000348_32 T7N9.5 [Arabidopsis thaliana] 116 4e-24
gb|AAF69161.1|AC007915_13 F27F5.19 [Arabidopsis thaliana] 115 5e-24
emb|CAA19696.1| putative LTR retrotransposon (fragment) [Arabido... 115 6e-24
gb|ABW74573.1| putative pol polyprotein [Boechera divaricarpa] 113 2e-23
emb|CAN76546.1| hypothetical protein VITISV_010420 [Vitis vinifera] 113 3e-23
emb|CAN83990.1| hypothetical protein VITISV_018454 [Vitis vinifera] 112 4e-23
>emb|CAN75186.1| hypothetical protein VITISV_001913 [Vitis vinifera]
Length = 746
Score = 160 bits (404), Expect = 2e-37
Identities = 77/109 (70%), Positives = 87/109 (79%)
Frame = -2
Query: 329 NKTDHCTICPLAKQRRLSFVSHNNLSKSRFDLVHCDT*GPYQVPTHAGYKYFLTLVDDCT 150
NK C ICPLAKQRR SF SHN++S+ FDLVHCD GPY V +HA ++YFLTL DDC+
Sbjct: 99 NKALPCYICPLAKQRRFSFDSHNHMSQFPFDLVHCDIWGPYSVSSHARHRYFLTLGDDCS 158
Query: 149 CFTWIFLMKQKSDVSYIIPKFFSMIETQFNIIIKQFRSDNAPELHFTDF 3
FTWIFL+KQKSDV IIPKFF+M+ TQFN IK FRSDNAPEL FTDF
Sbjct: 159 RFTWIFLLKQKSDVGTIIPKFFNMVVTQFNAKIKVFRSDNAPELAFTDF 207
>ref|XP_004149623.1| PREDICTED: uncharacterized protein LOC101211618 [Cucumis sativus]
Length = 2085
Score = 145 bits (366), Expect = 6e-33
Identities = 64/108 (59%), Positives = 86/108 (79%)
Frame = -2
Query: 329 NKTDHCTICPLAKQRRLSFVSHNNLSKSRFDLVHCDT*GPYQVPTHAGYKYFLTLVDDCT 150
N + C ICPLAKQRRLSF NN++++ FDL+HCD GP++ PTHAG+ YF T+VDD +
Sbjct: 1823 NCKEVCHICPLAKQRRLSFPILNNIAENIFDLIHCDIWGPFKTPTHAGHSYFATIVDDKS 1882
Query: 149 CFTWIFLMKQKSDVSYIIPKFFSMIETQFNIIIKQFRSDNAPELHFTD 6
+TW++L+K KSD+ +IP+FF +IETQF+ +IK FRSDNAPEL+F D
Sbjct: 1883 RYTWVYLLKHKSDILQVIPRFFKLIETQFSKVIKVFRSDNAPELNFRD 1930
Score = 137 bits (346), Expect = 1e-30
Identities = 60/101 (59%), Positives = 82/101 (81%)
Frame = -2
Query: 308 ICPLAKQRRLSFVSHNNLSKSRFDLVHCDT*GPYQVPTHAGYKYFLTLVDDCTCFTWIFL 129
ICPLAKQRRLSF NN++++ F+L+HCD GP++ PTHAG+ Y T+VDD + +TW++L
Sbjct: 405 ICPLAKQRRLSFPILNNIAENVFELIHCDIWGPFKTPTHAGHSYSATIVDDKSRYTWVYL 464
Query: 128 MKQKSDVSYIIPKFFSMIETQFNIIIKQFRSDNAPELHFTD 6
+K KSD+ +IP+FF +IETQF+ +IK FRSDNAPEL+F D
Sbjct: 465 LKHKSDILQVIPRFFKLIETQFSKVIKVFRSDNAPELNFRD 505
>ref|XP_004149403.1| PREDICTED: uncharacterized protein LOC101215551 [Cucumis sativus]
Length = 528
Score = 141 bits (355), Expect = 1e-31
Identities = 68/108 (62%), Positives = 80/108 (74%), Gaps = 1/108 (0%)
Frame = -2
Query: 323 TDH-CTICPLAKQRRLSFVSHNNLSKSRFDLVHCDT*GPYQVPTHAGYKYFLTLVDDCTC 147
T H CT C LAKQRRLSF S+N++S + FDL+H D GP THAG+ YFLT+VDD T
Sbjct: 361 TSHTCTTCSLAKQRRLSFSSNNHVSPNSFDLIHVDIWGPLSTATHAGHTYFLTIVDDATR 420
Query: 146 FTWIFLMKQKSDVSYIIPKFFSMIETQFNIIIKQFRSDNAPELHFTDF 3
FTW+F++KQKSDV IIP FF +IETQ IK RSDNAPEL FT+F
Sbjct: 421 FTWVFMLKQKSDVKQIIPNFFKLIETQHGKTIKHMRSDNAPELMFTEF 468
>gb|AAB61111.1| Strong similarity to Zea mays retrotransposon Hopscotch polyprotein
(gb|U12626) [Arabidopsis thaliana]
Length = 1315
Score = 137 bits (346), Expect = 1e-30
Identities = 63/111 (56%), Positives = 81/111 (72%), Gaps = 1/111 (0%)
Frame = -2
Query: 332 ENKTD-HCTICPLAKQRRLSFVSHNNLSKSRFDLVHCDT*GPYQVPTHAGYKYFLTLVDD 156
+N TD HC +C ++KQ+ L FVSHNN S FDL+H DT GP+ V TH GY+YFLT+VDD
Sbjct: 432 KNNTDFHCRVCHISKQKHLPFVSHNNKSSRPFDLIHIDTWGPFSVQTHDGYRYFLTIVDD 491
Query: 155 CTCFTWIFLMKQKSDVSYIIPKFFSMIETQFNIIIKQFRSDNAPELHFTDF 3
+ TW++L++ KSDV +IP F +M+E QF IK RSDNAPEL+FT F
Sbjct: 492 YSRATWVYLLRNKSDVLTVIPTFVTMVENQFETTIKGVRSDNAPELNFTQF 542
>dbj|BAB10503.1| retroelement pol polyprotein-like [Arabidopsis thaliana]
Length = 1475
Score = 134 bits (337), Expect = 1e-29
Identities = 62/108 (57%), Positives = 79/108 (73%), Gaps = 2/108 (1%)
Frame = -2
Query: 329 NKTD--HCTICPLAKQRRLSFVSHNNLSKSRFDLVHCDT*GPYQVPTHAGYKYFLTLVDD 156
NK D HC+IC AKQ+ LSF S NN+S+++FDL+H DT GP+ PT GYKYFLT+VDD
Sbjct: 611 NKEDLVHCSICQKAKQKHLSFPSKNNMSENKFDLIHIDTWGPFATPTTEGYKYFLTIVDD 670
Query: 155 CTCFTWIFLMKQKSDVSYIIPKFFSMIETQFNIIIKQFRSDNAPELHF 12
+ TW++LMK K+DV I P F M+ETQ+ ++K RSDNAPEL F
Sbjct: 671 YSRATWVYLMKAKNDVLQIFPDFLKMVETQYGTLVKAVRSDNAPELRF 718
>gb|AAC33963.1| contains similarity to reverse transcriptases (Pfam; rvt.hmm,
score: 11.19) [Arabidopsis thaliana]
Length = 1633
Score = 132 bits (331), Expect = 7e-29
Identities = 61/105 (58%), Positives = 77/105 (73%)
Frame = -2
Query: 317 HCTICPLAKQRRLSFVSHNNLSKSRFDLVHCDT*GPYQVPTHAGYKYFLTLVDDCTCFTW 138
HC I PLAKQ+RL++VSHNNL+ S FDL+H D GP+ + + G++YFLTLVDDCT TW
Sbjct: 531 HCRISPLAKQKRLAYVSHNNLASSPFDLIHLDIWGPFSIESVDGFRYFLTLVDDCTRTTW 590
Query: 137 IFLMKQKSDVSYIIPKFFSMIETQFNIIIKQFRSDNAPELHFTDF 3
+++MK KS+VS I P F +I TQ+N IK RSDN EL FT F
Sbjct: 591 VYMMKNKSEVSNIFPVFVKLIFTQYNAKIKAIRSDNVKELAFTKF 635
>emb|CAN64225.1| hypothetical protein VITISV_016222 [Vitis vinifera]
Length = 987
Score = 128 bits (321), Expect = 9e-28
Identities = 56/94 (59%), Positives = 73/94 (77%)
Frame = -2
Query: 287 RRLSFVSHNNLSKSRFDLVHCDT*GPYQVPTHAGYKYFLTLVDDCTCFTWIFLMKQKSDV 108
+RL F HNNLS S FDL+HCD GP+ +PTH G++YFLT+VDDCT TW+ L++ KSDV
Sbjct: 413 KRLPFDCHNNLSPSPFDLIHCDIWGPFHIPTHDGFRYFLTIVDDCTRNTWVHLLRAKSDV 472
Query: 107 SYIIPKFFSMIETQFNIIIKQFRSDNAPELHFTD 6
I P+FFSM++TQF + IK RSDNAPEL+ ++
Sbjct: 473 KTIFPQFFSMVKTQFGLTIKAVRSDNAPELNLSN 506
>emb|CAN65540.1| hypothetical protein VITISV_029946 [Vitis vinifera]
Length = 1240
Score = 125 bits (315), Expect = 5e-27
Identities = 55/94 (58%), Positives = 72/94 (76%)
Frame = -2
Query: 287 RRLSFVSHNNLSKSRFDLVHCDT*GPYQVPTHAGYKYFLTLVDDCTCFTWIFLMKQKSDV 108
+RL F HNNLS S FDL+HCD GP+ +PTH G++YFLT+VDDCT TW+ L++ KSDV
Sbjct: 413 KRLPFDCHNNLSPSPFDLIHCDIWGPFHIPTHDGFRYFLTIVDDCTRNTWVHLLRAKSDV 472
Query: 107 SYIIPKFFSMIETQFNIIIKQFRSDNAPELHFTD 6
I P+FFSM++TQF + IK RSDNAP L+ ++
Sbjct: 473 KTIFPQFFSMVKTQFGLTIKXVRSDNAPXLNLSN 506
>emb|CAA72989.1| unnamed protein product [Brassica oleracea var. viridis]
Length = 1131
Score = 122 bits (307), Expect = 4e-26
Identities = 59/110 (53%), Positives = 78/110 (70%), Gaps = 1/110 (0%)
Frame = -2
Query: 332 ENKTD-HCTICPLAKQRRLSFVSHNNLSKSRFDLVHCDT*GPYQVPTHAGYKYFLTLVDD 156
+NK D HC +C LAKQ++LS+ S N++ + F L+H D GP+ V T GYKYFLT+VDD
Sbjct: 543 KNKGDAHCHVCHLAKQKKLSYSSQNHICTASFQLLHVDVWGPFSVETLEGYKYFLTIVDD 602
Query: 155 CTCFTWIFLMKQKSDVSYIIPKFFSMIETQFNIIIKQFRSDNAPELHFTD 6
+ TWI+L++ KSDV +I P F + IETQ+N IK R DNAPEL FT+
Sbjct: 603 HSRATWIYLLQSKSDVLHIFPTFVNQIETQYNTKIKSVRRDNAPELSFTE 652
>gb|AAD25646.1| putative retroelement pol polyprotein [Arabidopsis thaliana]
Length = 1461
Score = 121 bits (304), Expect = 9e-26
Identities = 56/108 (51%), Positives = 76/108 (70%)
Frame = -2
Query: 326 KTDHCTICPLAKQRRLSFVSHNNLSKSRFDLVHCDT*GPYQVPTHAGYKYFLTLVDDCTC 147
K+ +C +C LAKQ++LSF S NN+ S F+L+H D GP+ V T GYKYFLT+VDD +
Sbjct: 597 KSAYCHVCHLAKQKKLSFPSANNICNSTFELLHIDVWGPFSVETVEGYKYFLTIVDDHSR 656
Query: 146 FTWIFLMKQKSDVSYIIPKFFSMIETQFNIIIKQFRSDNAPELHFTDF 3
TWI+L+K KSDV + P F ++E Q++ +K RSDNA EL FT+F
Sbjct: 657 ATWIYLLKSKSDVLTVFPAFIDLVENQYDTRVKSVRSDNAKELAFTEF 704
>gb|AAG10817.1|AC011808_5 Putative retroelement polyprotein [Arabidopsis thaliana]
Length = 1413
Score = 121 bits (303), Expect = 1e-25
Identities = 55/104 (52%), Positives = 73/104 (70%)
Frame = -2
Query: 317 HCTICPLAKQRRLSFVSHNNLSKSRFDLVHCDT*GPYQVPTHAGYKYFLTLVDDCTCFTW 138
HC IC AKQ++L++ S +N+ + FDL+H D GP+ PT GY YFLT+VDD T TW
Sbjct: 565 HCDICQRAKQKKLTYPSRHNICLAPFDLLHIDVWGPFSEPTQEGYHYFLTIVDDHTRVTW 624
Query: 137 IFLMKQKSDVSYIIPKFFSMIETQFNIIIKQFRSDNAPELHFTD 6
++LMK KSDV I P F +M+ETQ++ +K RSDNAPEL F +
Sbjct: 625 VYLMKYKSDVLTIFPDFITMVETQYDTKVKAVRSDNAPELKFEE 668
>emb|CAB10225.1| retrovirus-related like polyprotein [Arabidopsis thaliana]
gi|7268152|emb|CAB78488.1| retrovirus-related like
polyprotein [Arabidopsis thaliana]
Length = 1489
Score = 120 bits (300), Expect = 3e-25
Identities = 52/104 (50%), Positives = 75/104 (72%)
Frame = -2
Query: 317 HCTICPLAKQRRLSFVSHNNLSKSRFDLVHCDT*GPYQVPTHAGYKYFLTLVDDCTCFTW 138
H + L K +RL+++SHNNL+ + FDLVH D GP+ + + G++YFLT+VDDCT TW
Sbjct: 608 HPSSVVLQKLKRLAYISHNNLASNPFDLVHLDIWGPFSIESIEGFRYFLTVVDDCTRTTW 667
Query: 137 IFLMKQKSDVSYIIPKFFSMIETQFNIIIKQFRSDNAPELHFTD 6
+++++ K DVS + P+F ++ TQFN IK RSDNAPEL FT+
Sbjct: 668 VYMLRNKKDVSSVFPEFIKLVSTQFNAKIKAIRSDNAPELGFTE 711
>gb|AAD26943.1| putative retroelement pol polyprotein [Arabidopsis thaliana]
Length = 1454
Score = 120 bits (300), Expect = 3e-25
Identities = 55/107 (51%), Positives = 73/107 (68%)
Frame = -2
Query: 323 TDHCTICPLAKQRRLSFVSHNNLSKSRFDLVHCDT*GPYQVPTHAGYKYFLTLVDDCTCF 144
+D C +C LAKQR+LSF + N + K FDL+H D GP+ V T GYKYFLT+VDD +
Sbjct: 587 SDFCHVCHLAKQRKLSFPTSNKVCKEIFDLLHIDVWGPFSVETVEGYKYFLTIVDDHSRA 646
Query: 143 TWIFLMKQKSDVSYIIPKFFSMIETQFNIIIKQFRSDNAPELHFTDF 3
TW++L+K KS+V + P F +E Q+ + +K RSDNAPEL FT F
Sbjct: 647 TWMYLLKTKSEVLTVFPAFIQQVENQYKVKVKAVRSDNAPELKFTSF 693
>ref|XP_004245399.1| PREDICTED: uncharacterized protein LOC101252010 [Solanum
lycopersicum]
Length = 2121
Score = 119 bits (299), Expect = 3e-25
Identities = 51/108 (47%), Positives = 76/108 (70%)
Frame = -2
Query: 329 NKTDHCTICPLAKQRRLSFVSHNNLSKSRFDLVHCDT*GPYQVPTHAGYKYFLTLVDDCT 150
N+ +CTICPLAKQ RL F + + +S S F L+H D GP+ PT+ G ++F+T+VDDCT
Sbjct: 497 NEVHNCTICPLAKQPRLPFSNSSTISSSPFQLLHIDVWGPFHTPTYDGNRFFVTIVDDCT 556
Query: 149 CFTWIFLMKQKSDVSYIIPKFFSMIETQFNIIIKQFRSDNAPELHFTD 6
W+FL+K KSDV ++ FF++++TQFN+++K R+DN E T+
Sbjct: 557 RMLWLFLIKLKSDVCVVLKNFFTLVKTQFNLLVKSIRTDNGSEFVNTE 604
>gb|AAF79879.1|AC000348_32 T7N9.5 [Arabidopsis thaliana]
Length = 1436
Score = 116 bits (290), Expect = 4e-24
Identities = 53/106 (50%), Positives = 73/106 (68%)
Frame = -2
Query: 323 TDHCTICPLAKQRRLSFVSHNNLSKSRFDLVHCDT*GPYQVPTHAGYKYFLTLVDDCTCF 144
+ HC +C L+KQ+ L F S N++ + F+LVH DT GP+ VPT Y+YFLT+VDD +
Sbjct: 588 SSHCHVCHLSKQKHLPFKSVNHIREKAFELVHIDTWGPFSVPTVDSYRYFLTIVDDFSRA 647
Query: 143 TWIFLMKQKSDVSYIIPKFFSMIETQFNIIIKQFRSDNAPELHFTD 6
TWI+L+KQKSDV + P F M+ETQ++ + RSDNA EL F +
Sbjct: 648 TWIYLLKQKSDVLTVFPSFLKMVETQYHTKVCSVRSDNAHELKFNE 693
>gb|AAF69161.1|AC007915_13 F27F5.19 [Arabidopsis thaliana]
Length = 1309
Score = 115 bits (289), Expect = 5e-24
Identities = 54/103 (52%), Positives = 71/103 (68%)
Frame = -2
Query: 314 CTICPLAKQRRLSFVSHNNLSKSRFDLVHCDT*GPYQVPTHAGYKYFLTLVDDCTCFTWI 135
C +C L+KQ+ LSF S N+ K+ FDLVH DT GP+ V T G++YFLT+VDD + TW
Sbjct: 566 CRVCHLSKQKHLSFQSRQNMCKAAFDLVHIDTWGPFYVLTKDGFRYFLTIVDDFSRATWN 625
Query: 134 FLMKQKSDVSYIIPKFFSMIETQFNIIIKQFRSDNAPELHFTD 6
+L+K KSDV + P F +M+ TQ+ +K RSDNA EL FTD
Sbjct: 626 YLLKNKSDVLHAFPTFINMVHTQYQTKLKSVRSDNAHELKFTD 668
>emb|CAA19696.1| putative LTR retrotransposon (fragment) [Arabidopsis thaliana]
gi|7268775|emb|CAB78981.1| putative LTR retrotransposon
(fragment) [Arabidopsis thaliana]
Length = 281
Score = 115 bits (288), Expect = 6e-24
Identities = 52/99 (52%), Positives = 70/99 (70%)
Frame = -2
Query: 314 CTICPLAKQRRLSFVSHNNLSKSRFDLVHCDT*GPYQVPTHAGYKYFLTLVDDCTCFTWI 135
C +C L+KQ+ LSF N+ + FDLVH DT GP+ VPT+ G+KYFLT+VDD + TWI
Sbjct: 180 CHVCHLSKQKHLSFQYRQNMCSAAFDLVHIDTWGPFSVPTNDGFKYFLTIVDDFSRATWI 239
Query: 134 FLMKQKSDVSYIIPKFFSMIETQFNIIIKQFRSDNAPEL 18
+L+K KSDV ++ P F +M+ TQ+ +K RSDNA EL
Sbjct: 240 YLLKNKSDVLHVFPAFINMVHTQYQTKLKSVRSDNAHEL 278
>gb|ABW74573.1| putative pol polyprotein [Boechera divaricarpa]
Length = 937
Score = 113 bits (283), Expect = 2e-23
Identities = 52/108 (48%), Positives = 73/108 (67%)
Frame = -2
Query: 326 KTDHCTICPLAKQRRLSFVSHNNLSKSRFDLVHCDT*GPYQVPTHAGYKYFLTLVDDCTC 147
K+ +C I LAKQ +L +VS NN+ K F+L+H D GP+ V T G+KYFL +VDDC+
Sbjct: 506 KSAYCHIYHLAKQIKLFYVSPNNMCKIIFELLHIDVWGPFSVDTIEGFKYFLIIVDDCSR 565
Query: 146 FTWIFLMKQKSDVSYIIPKFFSMIETQFNIIIKQFRSDNAPELHFTDF 3
TW++L+K K++V + P F +E Q+ + +K RSDNAPEL FT F
Sbjct: 566 ATWVYLLKTKNEVLIVFPVFIQKVENQYGVKVKALRSDNAPELRFTKF 613
>emb|CAN76546.1| hypothetical protein VITISV_010420 [Vitis vinifera]
Length = 1288
Score = 113 bits (282), Expect = 3e-23
Identities = 51/104 (49%), Positives = 71/104 (68%)
Frame = -2
Query: 314 CTICPLAKQRRLSFVSHNNLSKSRFDLVHCDT*GPYQVPTHAGYKYFLTLVDDCTCFTWI 135
C +CPLAKQR L ++S N S FDL+H D GP+ V + GYK+FLT+VDD + TW+
Sbjct: 446 CNVCPLAKQRCLPYISLNKRCSSTFDLLHLDIWGPFSVGSVEGYKFFLTIVDDYSRVTWV 505
Query: 134 FLMKQKSDVSYIIPKFFSMIETQFNIIIKQFRSDNAPELHFTDF 3
+++K KS+V IP FF+ ++ QF +K RSDNAPEL ++F
Sbjct: 506 YMLKNKSEVQKYIPDFFAFVKKQFGKEVKAIRSDNAPELFLSNF 549
>emb|CAN83990.1| hypothetical protein VITISV_018454 [Vitis vinifera]
Length = 1243
Score = 112 bits (281), Expect = 4e-23
Identities = 51/104 (49%), Positives = 71/104 (68%)
Frame = -2
Query: 314 CTICPLAKQRRLSFVSHNNLSKSRFDLVHCDT*GPYQVPTHAGYKYFLTLVDDCTCFTWI 135
C +CPLAKQR L ++S N S FDL+H D GP+ V + GYK+FLT+VDD + TW+
Sbjct: 445 CXVCPLAKQRCLPYISLNKRCSSTFDLLHLDIWGPFSVGSVEGYKFFLTIVDDYSRVTWV 504
Query: 134 FLMKQKSDVSYIIPKFFSMIETQFNIIIKQFRSDNAPELHFTDF 3
+++K KS+V IP FF+ ++ QF +K RSDNAPEL ++F
Sbjct: 505 YMLKNKSEVQKYIPDFFAFVKKQFGKEVKAIRSDNAPELFLSNF 548