BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Akebia24_contig00021506
         (839 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Viti...   213   6e-53
ref|XP_007201750.1| hypothetical protein PRUPE_ppa001199mg [Prun...   201   4e-49
ref|XP_007050677.1| Debranching enzyme 1 [Theobroma cacao] gi|50...   174   3e-41
ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|2...   171   3e-40
ref|XP_006479763.1| PREDICTED: isoamylase 2, chloroplastic-like ...   161   3e-37
gb|EXB39337.1| Isoamylase 2 [Morus notabilis]                         158   3e-36
ref|XP_006386822.1| hypothetical protein POPTR_0002s22530g [Popu...   156   1e-35
ref|XP_006444132.1| hypothetical protein CICLE_v10023436mg [Citr...   154   4e-35
ref|XP_004292603.1| PREDICTED: isoamylase 2, chloroplastic-like ...   153   9e-35
ref|XP_006355876.1| PREDICTED: isoamylase 2, chloroplastic [Sola...   148   3e-33
ref|NP_001274804.1| isoamylase isoform 2 [Solanum tuberosum] gi|...   148   3e-33
ref|XP_004164565.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2...   146   1e-32
ref|XP_004136188.1| PREDICTED: isoamylase 2, chloroplastic-like ...   146   1e-32
ref|XP_003554240.1| PREDICTED: isoamylase 2, chloroplastic-like ...   145   2e-32
ref|XP_004247144.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2...   143   7e-32
ref|XP_007162398.1| hypothetical protein PHAVU_001G148700g [Phas...   141   3e-31
ref|XP_004493650.1| PREDICTED: isoamylase 2, chloroplastic-like ...   140   5e-31
ref|XP_003520557.1| PREDICTED: isoamylase 2, chloroplastic-like ...   139   1e-30
ref|XP_002889452.1| ATISA2/BE2/DBE1/ISA2 [Arabidopsis lyrata sub...   137   4e-30
gb|AAM98123.1| putative isoamylase [Arabidopsis thaliana]             136   9e-30

>ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Vitis vinifera]
          Length = 882

 Score =  213 bits (543), Expect = 6e-53
 Identities = 123/249 (49%), Positives = 157/249 (63%), Gaps = 11/249 (4%)
 Frame = +3

Query: 126 MATLPPSLTI----------SSVKLTKPTTSACFYDPNKEIHTQMDM-LGRKRVYKQCSQ 272
           M TL PSL I           S KL   T   C    NK  H    M L RK +    +Q
Sbjct: 1   MTTLIPSLVIRPCCVNCGARDSSKLVAATHYIC---GNKVAHGLEKMDLERKEILGVVAQ 57

Query: 273 GVTRNAHRDQVFKVFASTQRSAYQVGQELYTSTEVEELENASSYLFRTEIGGQVKVYIGR 452
             TRN  R+  +KV A T   A +  +  Y++TE EELE+  +YLF TEIGGQVKV +G+
Sbjct: 58  NATRNCFRNINWKVSA-TSHIAIEETENRYSTTESEELESRLNYLFLTEIGGQVKVIVGK 116

Query: 453 KNMKYVVYIEVSSLSQWSDTDELVLSWGMFRSDSSSLMPLDPQSSVPGTRSNTTQTQFMQ 632
           KN KY+V IEVSSL  ++  ++L+LSWG+FRS+SS  MP+D Q+ VP   SNTT+  FM+
Sbjct: 117 KNKKYIVSIEVSSLQLYNSDNKLILSWGVFRSNSSCFMPVDFQNLVPEVGSNTTEIPFME 176

Query: 633 KSLGKLALEMEFDLNQAPFYLSFLLHSVSDAATSNLEIRSHRHVDFCVPVGINSGYPAPL 812
           +S G  AL+++F+ N APFYLSFLL S  D   S+ +IRSHR  +FC+PVG   GYPAPL
Sbjct: 177 RSSGSFALKLDFEANHAPFYLSFLLKSTLDTDLSSSDIRSHRKTNFCIPVGFGRGYPAPL 236

Query: 813 GVSFSDDGS 839
           G+SFS DGS
Sbjct: 237 GLSFSSDGS 245


>ref|XP_007201750.1| hypothetical protein PRUPE_ppa001199mg [Prunus persica]
           gi|462397150|gb|EMJ02949.1| hypothetical protein
           PRUPE_ppa001199mg [Prunus persica]
          Length = 883

 Score =  201 bits (510), Expect = 4e-49
 Identities = 109/246 (44%), Positives = 151/246 (61%), Gaps = 8/246 (3%)
 Frame = +3

Query: 126 MATLPPSLTISS-------VKLTKPTTSACFYDPNKEIHTQMDM-LGRKRVYKQCSQGVT 281
           MATLP S+ I +        + +K T +  F   +K +H  + +   RK V+++  Q + 
Sbjct: 1   MATLPLSIAIQACCFNCGITESSKLTATNGFRHRDKAMHGFVKLDAERKLVFREVVQNIN 60

Query: 282 RNAHRDQVFKVFASTQRSAYQVGQELYTSTEVEELENASSYLFRTEIGGQVKVYIGRKNM 461
            N  RD   KV+A +Q S   + Q   T TE EE++  S+YLFRTEIG  V V++ ++N 
Sbjct: 61  ENLPRDHNLKVYAKSQVSVEPMEQRFSTGTETEEIDKVSTYLFRTEIGDVVNVFVRKRNA 120

Query: 462 KYVVYIEVSSLSQWSDTDELVLSWGMFRSDSSSLMPLDPQSSVPGTRSNTTQTQFMQKSL 641
           KY V IEV S    S+   LVL WGM+R+DSS  +PLD +SS P   + T +T  +Q S 
Sbjct: 121 KYTVNIEVPSSHLSSNDRRLVLRWGMYRADSSCFVPLDFKSSTPNDTTTTLETPLIQTSS 180

Query: 642 GKLALEMEFDLNQAPFYLSFLLHSVSDAATSNLEIRSHRHVDFCVPVGINSGYPAPLGVS 821
           G+  LE+EF+  Q PFY SF+L S +DA  S++EIRSHR  +FCVPVG   GYP PLG++
Sbjct: 181 GRFTLELEFEAKQIPFYFSFILTSPADANVSDMEIRSHRKTNFCVPVGFGRGYPGPLGLT 240

Query: 822 FSDDGS 839
           FS+DGS
Sbjct: 241 FSNDGS 246


>ref|XP_007050677.1| Debranching enzyme 1 [Theobroma cacao] gi|508702938|gb|EOX94834.1|
           Debranching enzyme 1 [Theobroma cacao]
          Length = 867

 Score =  174 bits (442), Expect = 3e-41
 Identities = 106/248 (42%), Positives = 149/248 (60%), Gaps = 10/248 (4%)
 Frame = +3

Query: 126 MATLPPSLTISS----------VKLTKPTTSACFYDPNKEIHTQMDMLGRKRVYKQCSQG 275
           MATLPPSL I             KL+  T   C     + +  Q   +GRK    + +Q 
Sbjct: 1   MATLPPSLAIGPHCLNSGATELSKLSFVTRYLCKSKMGQSL--QRIDVGRKLFSGEVAQN 58

Query: 276 VTRNAHRDQVFKVFASTQRSAYQVGQELYTSTEVEELENASSYLFRTEIGGQVKVYIGRK 455
           V ++ + +   + FA+++ S  Q  ++++TST+V+EL+  S+YLFRTEIGGQVKV++ ++
Sbjct: 59  VMQSRYWNLDLRCFAASRVSVEQT-EQIFTSTQVDELKKLSTYLFRTEIGGQVKVFVRKR 117

Query: 456 NMKYVVYIEVSSLSQWSDTDELVLSWGMFRSDSSSLMPLDPQSSVPGTRSNTTQTQFMQK 635
           ++ +VV IEVSSL    D  +LVLS G+ RSD                ++   +T F+ K
Sbjct: 118 SVNHVVDIEVSSLQLSGDESQLVLSGGIHRSDHD-------------IKNRIIETPFIAK 164

Query: 636 SLGKLALEMEFDLNQAPFYLSFLLHSVSDAATSNLEIRSHRHVDFCVPVGINSGYPAPLG 815
           S  +LALE+EF+  +APFY SFLL + S A  S  EIR+HR  +FCVPVG N GYPAPLG
Sbjct: 165 SSSELALELEFEAKEAPFYFSFLLKAPSGANLSGSEIRTHRKTNFCVPVGFNQGYPAPLG 224

Query: 816 VSFSDDGS 839
           +SFS DGS
Sbjct: 225 LSFSTDGS 232


>ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|223527143|gb|EEF29318.1|
           isoamylase, putative [Ricinus communis]
          Length = 872

 Score =  171 bits (433), Expect = 3e-40
 Identities = 102/243 (41%), Positives = 138/243 (56%), Gaps = 5/243 (2%)
 Frame = +3

Query: 126 MATLPPSLTISSVKLTKPTTSAC-FYDPNKEIHTQMDMLGR----KRVYKQCSQGVTRNA 290
           MAT+ PS  I      +P    C   + +K   T     GR    +R++ Q      ++ 
Sbjct: 1   MATIIPSFAI------RPCYCVCGATESSKLAVTGNKDFGRLVLDQRLFNQRLHFWKQSP 54

Query: 291 HRDQVFKVFASTQRSAYQVGQELYTSTEVEELENASSYLFRTEIGGQVKVYIGRKNMKYV 470
           H     +V ++ +    Q      TS  ++EL   S+YLFRT+ GG VKV + +KN KY 
Sbjct: 55  HWSLDSRVCSAARVPVQQTELRFSTSAPLDELNKVSTYLFRTQFGGHVKVLVRKKNAKYA 114

Query: 471 VYIEVSSLSQWSDTDELVLSWGMFRSDSSSLMPLDPQSSVPGTRSNTTQTQFMQKSLGKL 650
           VYIEVSSL   +    L+L WG++RSDSS  MPLD Q+  P  R     T  +Q S G  
Sbjct: 115 VYIEVSSLELGTTDYRLMLIWGIYRSDSSCFMPLDSQNFAPNARK--MDTALVQNSFGTF 172

Query: 651 ALEMEFDLNQAPFYLSFLLHSVSDAATSNLEIRSHRHVDFCVPVGINSGYPAPLGVSFSD 830
           ALE+EF+  Q PFYLSFLL S  +   S LEI++H++ +FCVP+G NSG P+PLG+SFS 
Sbjct: 173 ALELEFEPKQTPFYLSFLLKSKLNTDASGLEIKNHKNANFCVPIGFNSGDPSPLGLSFST 232

Query: 831 DGS 839
           DGS
Sbjct: 233 DGS 235


>ref|XP_006479763.1| PREDICTED: isoamylase 2, chloroplastic-like isoform X1 [Citrus
           sinensis] gi|568852193|ref|XP_006479764.1| PREDICTED:
           isoamylase 2, chloroplastic-like isoform X2 [Citrus
           sinensis] gi|568852195|ref|XP_006479765.1| PREDICTED:
           isoamylase 2, chloroplastic-like isoform X3 [Citrus
           sinensis]
          Length = 889

 Score =  161 bits (407), Expect = 3e-37
 Identities = 90/201 (44%), Positives = 123/201 (61%), Gaps = 2/201 (0%)
 Frame = +3

Query: 243 RKRVYKQCSQGVTRNAHRDQV-FKVFASTQRSAYQVGQELYT-STEVEELENASSYLFRT 416
           R  V K  S  V +NA   +  F+  AS+ R +    Q ++T +TEV+EL+  S+YLFRT
Sbjct: 50  RLEVEKTLSAQVCQNAVTTRPDFRACASSSRVSVDETQPVFTPTTEVDELQKVSTYLFRT 109

Query: 417 EIGGQVKVYIGRKNMKYVVYIEVSSLSQWSDTDELVLSWGMFRSDSSSLMPLDPQSSVPG 596
           ++GG V V +G KN+ Y V I+VSSL       +LVL WG++ SDSSS M L  Q   P 
Sbjct: 110 DLGGLVSVSVGEKNVNYAVTIDVSSLQTTRGGHKLVLIWGVYTSDSSSYMHLGSQCFTPD 169

Query: 597 TRSNTTQTQFMQKSLGKLALEMEFDLNQAPFYLSFLLHSVSDAATSNLEIRSHRHVDFCV 776
            ++ + +T F   S    ALE+ F+  Q PFYLSFLL S     + + EIR+H    FCV
Sbjct: 170 AKTGSIETPFTPTSFDTFALELGFEAKQTPFYLSFLLKSPLRDGSGDFEIRNHLSAKFCV 229

Query: 777 PVGINSGYPAPLGVSFSDDGS 839
           PVG+N+G P+P+G+SFS DGS
Sbjct: 230 PVGLNAGVPSPMGLSFSIDGS 250


>gb|EXB39337.1| Isoamylase 2 [Morus notabilis]
          Length = 825

 Score =  158 bits (399), Expect = 3e-36
 Identities = 84/182 (46%), Positives = 117/182 (64%)
 Frame = +3

Query: 294 RDQVFKVFASTQRSAYQVGQELYTSTEVEELENASSYLFRTEIGGQVKVYIGRKNMKYVV 473
           R+   +V A+++ S   V + L  +T+VEE E  S+YLFRTE+GG V V++ +K +   V
Sbjct: 17  RNLSVEVNATSRVSIEPVQKRLSRNTKVEEAEKVSTYLFRTEVGGLVNVFVRKKKVNCTV 76

Query: 474 YIEVSSLSQWSDTDELVLSWGMFRSDSSSLMPLDPQSSVPGTRSNTTQTQFMQKSLGKLA 653
           Y+EVSSL        L LSWG++RSDSSS +P   ++S P       +T F + S G+  
Sbjct: 77  YVEVSSLELPRSDGTLGLSWGIYRSDSSSFLPSHFETSTP------VETPFTKNSFGRYT 130

Query: 654 LEMEFDLNQAPFYLSFLLHSVSDAATSNLEIRSHRHVDFCVPVGINSGYPAPLGVSFSDD 833
           +E EF+  + P YLSFLL S  D  +S L+IRSHR  +FCVP+G +SG+P PLG+SFS D
Sbjct: 131 VEFEFEAKEIPCYLSFLLKSPRDNDSSGLDIRSHRKTNFCVPLGFSSGHPTPLGLSFSRD 190

Query: 834 GS 839
           GS
Sbjct: 191 GS 192


>ref|XP_006386822.1| hypothetical protein POPTR_0002s22530g [Populus trichocarpa]
           gi|550345605|gb|ERP64619.1| hypothetical protein
           POPTR_0002s22530g [Populus trichocarpa]
          Length = 857

 Score =  156 bits (394), Expect = 1e-35
 Identities = 104/250 (41%), Positives = 136/250 (54%), Gaps = 12/250 (4%)
 Frame = +3

Query: 126 MATLPPSLTISSVKLTKPTTSACF-----YDPNKEIHTQMDMLGRKRVYKQCSQGVTRNA 290
           M TL P L I         T  CF      + +K IH          V K      TR  
Sbjct: 1   MTTLSPLLAI---------TPLCFCNCGSVESSKSIHY---------VCKTKKSRPTRRF 42

Query: 291 HRDQVFK----VFASTQRSAYQVGQELYTSTE--VEELENASSYLFRTEIGGQVKVYIGR 452
            R  V K    +FA ++ +A    Q ++TS+   V++L+  S+Y FRTEIGG VK+ +G+
Sbjct: 43  GRMDVGKDSRLLFARSRVAAQPTEQMMFTSSNPLVDQLKEVSTYRFRTEIGGHVKISVGK 102

Query: 453 KNMKYVVYIEVSSLSQW-SDTDELVLSWGMFRSDSSSLMPLDPQSSVPGTRSNTTQTQFM 629
            N KY VY+EVSSL    SD   L+L WG++ SDSS  MPLD  S      + T +T  +
Sbjct: 103 INGKYAVYVEVSSLELGASDNISLMLIWGIYTSDSSCFMPLDSSS-----HARTRETPLL 157

Query: 630 QKSLGKLALEMEFDLNQAPFYLSFLLHSVSDAATSNLEIRSHRHVDFCVPVGINSGYPAP 809
           Q S  + A E+EF+  Q PFYLSF L   S    S +EIR+H   +FCVP+G +SGYP P
Sbjct: 158 QNSCARFATELEFEAKQTPFYLSFFLKPTS----SVVEIRNHNKSNFCVPIGFDSGYPTP 213

Query: 810 LGVSFSDDGS 839
           LG+SFS DGS
Sbjct: 214 LGLSFSTDGS 223


>ref|XP_006444132.1| hypothetical protein CICLE_v10023436mg [Citrus clementina]
           gi|557546394|gb|ESR57372.1| hypothetical protein
           CICLE_v10023436mg [Citrus clementina]
          Length = 840

 Score =  154 bits (389), Expect = 4e-35
 Identities = 82/174 (47%), Positives = 108/174 (62%)
 Frame = +3

Query: 318 ASTQRSAYQVGQELYTSTEVEELENASSYLFRTEIGGQVKVYIGRKNMKYVVYIEVSSLS 497
           AS   S Y V      +TEV+EL+  S+YLFRT++GG V V +G KN+ Y V I+VSSL 
Sbjct: 31  ASASASHYPV---FTPTTEVDELQKVSTYLFRTDLGGLVSVSVGEKNVNYAVTIDVSSLQ 87

Query: 498 QWSDTDELVLSWGMFRSDSSSLMPLDPQSSVPGTRSNTTQTQFMQKSLGKLALEMEFDLN 677
                 +LVL WG++ SDSSS M L  Q   P  ++ + +T F   S    ALE+ F+  
Sbjct: 88  TTRGGHKLVLIWGVYTSDSSSYMHLGSQCFTPDAKTGSIETPFTPTSFDTFALELGFEAK 147

Query: 678 QAPFYLSFLLHSVSDAATSNLEIRSHRHVDFCVPVGINSGYPAPLGVSFSDDGS 839
           Q PFYLSFLL S     + + EIR+H    FCVPVG+N+G P+P+G+SFS DGS
Sbjct: 148 QTPFYLSFLLKSPLRDGSGDFEIRNHLSAKFCVPVGLNAGVPSPMGLSFSIDGS 201


>ref|XP_004292603.1| PREDICTED: isoamylase 2, chloroplastic-like [Fragaria vesca subsp.
           vesca]
          Length = 868

 Score =  153 bits (386), Expect = 9e-35
 Identities = 88/199 (44%), Positives = 116/199 (58%)
 Frame = +3

Query: 243 RKRVYKQCSQGVTRNAHRDQVFKVFASTQRSAYQVGQELYTSTEVEELENASSYLFRTEI 422
           RK V+    Q   +++ R    +V A++  S   + Q   T TE  E    S+YLFRTE 
Sbjct: 46  RKLVFGGFGQSFAQSSLRGCHSRVQAASGVSIEPMEQNFPTGTE--ETNKVSTYLFRTEN 103

Query: 423 GGQVKVYIGRKNMKYVVYIEVSSLSQWSDTDELVLSWGMFRSDSSSLMPLDPQSSVPGTR 602
           G  + V++    + Y VY+E+SSL   S  D LV+SWGM+R+DSSSL PLD         
Sbjct: 104 GDLINVFVRDNTVNYSVYVELSSLQLSSAGDRLVISWGMYRADSSSLKPLD--------- 154

Query: 603 SNTTQTQFMQKSLGKLALEMEFDLNQAPFYLSFLLHSVSDAATSNLEIRSHRHVDFCVPV 782
               +T F + S G   LE+EF+  Q PFYLSF + S++DA  S LEIRSHR  +FCVPV
Sbjct: 155 --FIETPFTKTSSGSFTLELEFEAKQTPFYLSFTVKSLADANLSGLEIRSHRKTNFCVPV 212

Query: 783 GINSGYPAPLGVSFSDDGS 839
           G   G P PLG+S+S DGS
Sbjct: 213 GFGRGCPTPLGLSYSSDGS 231


>ref|XP_006355876.1| PREDICTED: isoamylase 2, chloroplastic [Solanum tuberosum]
          Length = 878

 Score =  148 bits (373), Expect = 3e-33
 Identities = 75/155 (48%), Positives = 102/155 (65%)
 Frame = +3

Query: 375 VEELENASSYLFRTEIGGQVKVYIGRKNMKYVVYIEVSSLSQWSDTDELVLSWGMFRSDS 554
           V   +   +YLFRT+IGGQVKV + R N KY V +EV  L       ELV+ WG+FRSD+
Sbjct: 79  VPSAKRVPTYLFRTDIGGQVKVLVERTNGKYKVLVEVLPLELSYAHSELVMVWGLFRSDA 138

Query: 555 SSLMPLDPQSSVPGTRSNTTQTQFMQKSLGKLALEMEFDLNQAPFYLSFLLHSVSDAATS 734
           S  MPLD        +S+T +T F+Q   GK+ +E++F+ + APFY+SF + S   +   
Sbjct: 139 SCFMPLDLNRRGADGKSSTVETPFVQGPSGKVTVELDFEASLAPFYISFYMKSQLVSDME 198

Query: 735 NLEIRSHRHVDFCVPVGINSGYPAPLGVSFSDDGS 839
           N EIRSHR+ +F VPVG++SG+PAPLG+SF  DGS
Sbjct: 199 NSEIRSHRNTNFVVPVGLSSGHPAPLGISFQPDGS 233


>ref|NP_001274804.1| isoamylase isoform 2 [Solanum tuberosum] gi|27728147|gb|AAN15318.1|
           isoamylase isoform 2 [Solanum tuberosum]
          Length = 878

 Score =  148 bits (373), Expect = 3e-33
 Identities = 75/155 (48%), Positives = 102/155 (65%)
 Frame = +3

Query: 375 VEELENASSYLFRTEIGGQVKVYIGRKNMKYVVYIEVSSLSQWSDTDELVLSWGMFRSDS 554
           V   +   +YLFRT+IGGQVKV + R N KY V +EV  L       ELV+ WG+FRSD+
Sbjct: 79  VPSAKRVPTYLFRTDIGGQVKVLVERTNGKYKVLVEVLPLELSYAHSELVMVWGLFRSDA 138

Query: 555 SSLMPLDPQSSVPGTRSNTTQTQFMQKSLGKLALEMEFDLNQAPFYLSFLLHSVSDAATS 734
           S  MPLD        +S+T +T F+Q   GK+ +E++F+ + APFY+SF + S   +   
Sbjct: 139 SCFMPLDLNRRGADGKSSTVETPFVQGPSGKVTVELDFEASLAPFYISFYMKSQLVSDME 198

Query: 735 NLEIRSHRHVDFCVPVGINSGYPAPLGVSFSDDGS 839
           N EIRSHR+ +F VPVG++SG+PAPLG+SF  DGS
Sbjct: 199 NSEIRSHRNTNFVVPVGLSSGHPAPLGISFQPDGS 233


>ref|XP_004164565.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2, chloroplastic-like
           [Cucumis sativus]
          Length = 885

 Score =  146 bits (368), Expect = 1e-32
 Identities = 78/189 (41%), Positives = 116/189 (61%)
 Frame = +3

Query: 273 GVTRNAHRDQVFKVFASTQRSAYQVGQELYTSTEVEELENASSYLFRTEIGGQVKVYIGR 452
           G  +++HR+ + K +A +  S  + GQ L    + +E    ++YLFRTE G  V V++G+
Sbjct: 59  GAVKSSHRN-LSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGK 117

Query: 453 KNMKYVVYIEVSSLSQWSDTDELVLSWGMFRSDSSSLMPLDPQSSVPGTRSNTTQTQFMQ 632
           K   + V IEV S+   S  + L+LSWG++RSDS+ + P + +SS P   +  T+T F++
Sbjct: 118 KGSTFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTP-NFESSPPDETTGATETPFVK 176

Query: 633 KSLGKLALEMEFDLNQAPFYLSFLLHSVSDAATSNLEIRSHRHVDFCVPVGINSGYPAPL 812
            S GK ++E+EFD    PFYLSF+L       + + EIRSH+   F VPVG   GYP+PL
Sbjct: 177 TSEGKFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPL 236

Query: 813 GVSFSDDGS 839
           G+S S DGS
Sbjct: 237 GLSISGDGS 245


>ref|XP_004136188.1| PREDICTED: isoamylase 2, chloroplastic-like [Cucumis sativus]
          Length = 885

 Score =  146 bits (368), Expect = 1e-32
 Identities = 78/189 (41%), Positives = 116/189 (61%)
 Frame = +3

Query: 273 GVTRNAHRDQVFKVFASTQRSAYQVGQELYTSTEVEELENASSYLFRTEIGGQVKVYIGR 452
           G  +++HR+ + K +A +  S  + GQ L    + +E    ++YLFRTE G  V V++G+
Sbjct: 59  GAVKSSHRN-LSKTYAKSGISVGKSGQRLGIGGKSKEQRRVATYLFRTEFGDLVNVFVGK 117

Query: 453 KNMKYVVYIEVSSLSQWSDTDELVLSWGMFRSDSSSLMPLDPQSSVPGTRSNTTQTQFMQ 632
           K   + V IEV S+   S  + L+LSWG++RSDS+ + P + +SS P   +  T+T F++
Sbjct: 118 KGSTFTVNIEVPSMQLVSIDEALLLSWGVYRSDSALVTP-NFESSPPDETTGATETPFVK 176

Query: 633 KSLGKLALEMEFDLNQAPFYLSFLLHSVSDAATSNLEIRSHRHVDFCVPVGINSGYPAPL 812
            S GK ++E+EFD    PFYLSF+L       + + EIRSH+   F VPVG   GYP+PL
Sbjct: 177 TSEGKFSVELEFDAKHTPFYLSFVLKYPMGVDSGSSEIRSHKKTSFSVPVGFGRGYPSPL 236

Query: 813 GVSFSDDGS 839
           G+S S DGS
Sbjct: 237 GLSISGDGS 245


>ref|XP_003554240.1| PREDICTED: isoamylase 2, chloroplastic-like [Glycine max]
          Length = 865

 Score =  145 bits (365), Expect = 2e-32
 Identities = 83/179 (46%), Positives = 110/179 (61%), Gaps = 2/179 (1%)
 Frame = +3

Query: 309 KVFASTQRSAYQVGQELYTSTEVEELENASSYLFRTEIGGQ-VKVYIGRKNMKYVVYIEV 485
           K+ A+++ S  +  Q++ T T  E+L+ + +YLFRTE GG  VKV++ R+N +Y VYIE+
Sbjct: 56  KLCATSRLSIEETEQQIGTLTRPEDLKGSLAYLFRTETGGGLVKVHVTRRNDRYSVYIEI 115

Query: 486 SSLSQWSDTDE-LVLSWGMFRSDSSSLMPLDPQSSVPGTRSNTTQTQFMQKSLGKLALEM 662
           SSL       E L+L WG++RSDSS  + LD              +  +Q S GK A+E+
Sbjct: 116 SSLDISGGVGEALLLCWGVYRSDSSCFVDLDTIGLSENAAMGMNVSPLVQNSDGKFAIEL 175

Query: 663 EFDLNQAPFYLSFLLHSVSDAATSNLEIRSHRHVDFCVPVGINSGYPAPLGVSFSDDGS 839
           EFD    P YLSF L S  D   S LEIRSHR  +FC+PVG   GYP PLGVS+S DGS
Sbjct: 176 EFDAKHVPLYLSFFLMSSLD---SGLEIRSHRRTNFCMPVGSLPGYPCPLGVSYSPDGS 231


>ref|XP_004247144.1| PREDICTED: LOW QUALITY PROTEIN: isoamylase 2, chloroplastic-like
           [Solanum lycopersicum]
          Length = 878

 Score =  143 bits (361), Expect = 7e-32
 Identities = 71/154 (46%), Positives = 101/154 (65%)
 Frame = +3

Query: 375 VEELENASSYLFRTEIGGQVKVYIGRKNMKYVVYIEVSSLSQWSDTDELVLSWGMFRSDS 554
           V   +   +YLFRT+IG QVKV++ + N KY V +EV  L       ELV+ WG+FRSD+
Sbjct: 79  VPSAKRVPTYLFRTDIGCQVKVFVEKTNGKYKVLVEVLPLELSDAHSELVMVWGLFRSDA 138

Query: 555 SSLMPLDPQSSVPGTRSNTTQTQFMQKSLGKLALEMEFDLNQAPFYLSFLLHSVSDAATS 734
           S  MPLD        +S+T +T F+Q   GK+ +E++F+++  PFY+SF + S   +   
Sbjct: 139 SCFMPLDLNRRGADGKSSTVETPFVQGPSGKVTVELDFEVSLVPFYISFYMKSQLVSDME 198

Query: 735 NLEIRSHRHVDFCVPVGINSGYPAPLGVSFSDDG 836
           N EIRSHR+ +F VPVG++SG+PAPLG+SF  DG
Sbjct: 199 NSEIRSHRNTNFVVPVGLSSGHPAPLGISFQPDG 232


>ref|XP_007162398.1| hypothetical protein PHAVU_001G148700g [Phaseolus vulgaris]
           gi|139867055|dbj|BAF52942.1| isoamylase-type
           starch-debranching enzyme 2 [Phaseolus vulgaris]
           gi|561035862|gb|ESW34392.1| hypothetical protein
           PHAVU_001G148700g [Phaseolus vulgaris]
          Length = 865

 Score =  141 bits (356), Expect = 3e-31
 Identities = 80/185 (43%), Positives = 111/185 (60%), Gaps = 3/185 (1%)
 Frame = +3

Query: 294 RDQVFKVFASTQRSAYQVGQELYTSTEVEELENASSYLFRTEIGGQ-VKVYIGRKNMKYV 470
           R+   K+ A+++ S  +  Q++   T  E+L+ A +YLFRTE GG  VKVY+ +K  +Y 
Sbjct: 50  RNPSSKLCATSRLSIEETEQQVEPFTRPEDLKGALAYLFRTETGGGLVKVYVTKKKDRYF 109

Query: 471 VYIEVSSL--SQWSDTDELVLSWGMFRSDSSSLMPLDPQSSVPGTRSNTTQTQFMQKSLG 644
           VYIE+SSL  +   D++ LVL WG++R DSS  + +D              +  +Q S+ 
Sbjct: 110 VYIEISSLDVNHCGDSETLVLCWGVYRGDSSCFVDMDSTGLSGNAAKRMNVSPLVQTSVC 169

Query: 645 KLALEMEFDLNQAPFYLSFLLHSVSDAATSNLEIRSHRHVDFCVPVGINSGYPAPLGVSF 824
           K  +E+EFD    P YLSF L S  DA    LEI SHR  +FCVPVG+  GYP PLG+S+
Sbjct: 170 KFGVELEFDAKYVPLYLSFFLMSSLDA---GLEIISHRRTNFCVPVGLLPGYPGPLGLSY 226

Query: 825 SDDGS 839
           S DGS
Sbjct: 227 SPDGS 231


>ref|XP_004493650.1| PREDICTED: isoamylase 2, chloroplastic-like [Cicer arietinum]
          Length = 858

 Score =  140 bits (354), Expect = 5e-31
 Identities = 95/244 (38%), Positives = 133/244 (54%), Gaps = 6/244 (2%)
 Frame = +3

Query: 126 MATLPPSLTISSVKL----TKPTTSACFYDPNKEIHTQMDMLGRKRVYKQCSQGVTRNAH 293
           MA L PS + S + L       T  + F+ P  +I  Q  ++G     K     +     
Sbjct: 1   MANLLPSFSFSLIPLDHHHNHGTKCSFFHTPFIQIKHQHQLVG-----KNLQDLIISPFS 55

Query: 294 RDQVFKVFASTQRSAYQVGQELYTSTEVEELENASSYLFRTEIG-GQVKVYIGRKNMKYV 470
            +   K+ A+++ S  +  Q+  T T+ E+++ A SYLFRTEIG G VKVY+ +K + Y 
Sbjct: 56  HNLTSKLCATSRLSVEETEQKFSTFTQSEDIKTALSYLFRTEIGEGLVKVYVKKKKVTYF 115

Query: 471 VYIEVSSLSQW-SDTDELVLSWGMFRSDSSSLMPLDPQSSVPGTRSNTTQTQFMQKSLGK 647
           VYIEVSSL    ++ + LVL WG++R DS S+                  + F+Q SLGK
Sbjct: 116 VYIEVSSLELVRAEGETLVLCWGVYRDDSLSV----------NVGKGMNVSPFVQNSLGK 165

Query: 648 LALEMEFDLNQAPFYLSFLLHSVSDAATSNLEIRSHRHVDFCVPVGINSGYPAPLGVSFS 827
            ++E+EFD+ Q PFYLSFLL        S LEIR+H   +FCVPVG   G P+ LG+SFS
Sbjct: 166 FSVELEFDVEQVPFYLSFLLR------YSGLEIRTHMKRNFCVPVGFYKGCPSLLGLSFS 219

Query: 828 DDGS 839
            D S
Sbjct: 220 PDES 223


>ref|XP_003520557.1| PREDICTED: isoamylase 2, chloroplastic-like [Glycine max]
          Length = 866

 Score =  139 bits (351), Expect = 1e-30
 Identities = 93/243 (38%), Positives = 131/243 (53%), Gaps = 5/243 (2%)
 Frame = +3

Query: 126 MATLPPSLTISSVK---LTKPTTSACFYDPNKEIHTQMDMLGRKRVYKQCSQGVTRNAHR 296
           M +L P  + S +     +KP  S CF++P  +       LG +      S+  T +   
Sbjct: 1   MTSLAPRFSFSVIPGSHHSKPKFS-CFHEPFIQRKHLWPKLGLQNPISPFSRNPTSS--- 56

Query: 297 DQVFKVFASTQRSAYQVGQELYTSTEVEELENASSYLFRTEIGGQ-VKVYIGRKNMKYVV 473
               K+ A+++ S  +  Q+L T +  E L+ +S+YLFRTE GG  VKVY+ ++  +Y V
Sbjct: 57  ----KLCATSRLSIEETEQQLGTLSRPEYLKGSSAYLFRTETGGGLVKVYVTKRKDRYFV 112

Query: 474 YIEVSSLSQWSDTDE-LVLSWGMFRSDSSSLMPLDPQSSVPGTRSNTTQTQFMQKSLGKL 650
           Y E+SSL       E L+L WG++RSDSS  + LD         +    +  +Q S G  
Sbjct: 113 YTEISSLDISGSVGETLLLCWGVYRSDSSCFVDLDTIGLRENAATGMNVSPLVQNSDGNF 172

Query: 651 ALEMEFDLNQAPFYLSFLLHSVSDAATSNLEIRSHRHVDFCVPVGINSGYPAPLGVSFSD 830
           A+E+EFD    P YLSF L S  DA    +EIRSHR  +FCVPVG+  GYP PLG+  S 
Sbjct: 173 AVELEFDAKHVPLYLSFFLMSSLDA---GMEIRSHRGTNFCVPVGLLPGYPGPLGLFCSP 229

Query: 831 DGS 839
           DGS
Sbjct: 230 DGS 232


>ref|XP_002889452.1| ATISA2/BE2/DBE1/ISA2 [Arabidopsis lyrata subsp. lyrata]
           gi|297335294|gb|EFH65711.1| ATISA2/BE2/DBE1/ISA2
           [Arabidopsis lyrata subsp. lyrata]
          Length = 872

 Score =  137 bits (346), Expect = 4e-30
 Identities = 87/230 (37%), Positives = 131/230 (56%), Gaps = 4/230 (1%)
 Frame = +3

Query: 156 SSVKLTKPTTSACFYDPNKEIHTQMD--MLGRKRVYKQCSQGVTRNAHRDQVFKVFASTQ 329
           S V  T    SA F+    +I    +  M  RKR        VT      Q+ K+ AS  
Sbjct: 7   SGVTRTWKFPSARFFTGKNKIKLGSETLMFTRKRFMGDL---VTSALKSYQLSKICAS-- 61

Query: 330 RSAYQVGQELYTS-TEVEELENASSYLFRTEIGGQVKVYIGRKNMKYVVYIEVSSLS-QW 503
           +++ ++ + L T   +V++L+  +SY FRT+ G  VKV + +K  KY + + VSSL    
Sbjct: 62  KTSIELREALSTKRAQVDDLKKVTSYSFRTKSGALVKVKVEQKREKYSIMVYVSSLELTG 121

Query: 504 SDTDELVLSWGMFRSDSSSLMPLDPQSSVPGTRSNTTQTQFMQKSLGKLALEMEFDLNQA 683
            D   LV+ WG++RSDSS  +PLD ++S   ++++TT+T F++ SL +L L +EFD  ++
Sbjct: 122 DDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETPFVKSSLSELMLGLEFDGKES 181

Query: 684 PFYLSFLLHSVSDAATSNLEIRSHRHVDFCVPVGINSGYPAPLGVSFSDD 833
           PFYLSF L  VS       E+ +HR  DFC+PVG  +G+P PLG+S   D
Sbjct: 182 PFYLSFHLKLVSGRDPDGQEMLTHRDTDFCIPVGFTAGHPLPLGLSSGPD 231


>gb|AAM98123.1| putative isoamylase [Arabidopsis thaliana]
          Length = 882

 Score =  136 bits (343), Expect = 9e-30
 Identities = 90/247 (36%), Positives = 136/247 (55%), Gaps = 11/247 (4%)
 Frame = +3

Query: 126 MATLPPSLTISSVKLTKPTT------SACFYDPNK---EIHTQMDMLGRKRVYKQCSQGV 278
           MA   PS+ I S  L    T      SA  +   K   ++ ++  M  RKR        V
Sbjct: 1   MAAWSPSVGIGSCCLNNGITRTWKFPSARLFTGRKNKIKLGSETLMFTRKRFMGDL---V 57

Query: 279 TRNAHRDQVFKVFASTQRSAYQVGQELYTS-TEVEELENASSYLFRTEIGGQVKVYIGRK 455
           T      Q  K+ AS  +++ ++ + L +   E ++L+  +SY FRT+ G  VKV + +K
Sbjct: 58  TSALQSYQFSKICAS--KTSIELREALSSRRAEADDLKKVTSYSFRTKAGALVKVKVEKK 115

Query: 456 NMKYVVYIEVSSLS-QWSDTDELVLSWGMFRSDSSSLMPLDPQSSVPGTRSNTTQTQFMQ 632
             KY + + VSSL     D   LV+ WG++RSDSS  +PLD ++S   ++++TT+T F++
Sbjct: 116 REKYSILVYVSSLELSGDDKSRLVMVWGVYRSDSSCFLPLDFENSSQDSQTHTTETTFVK 175

Query: 633 KSLGKLALEMEFDLNQAPFYLSFLLHSVSDAATSNLEIRSHRHVDFCVPVGINSGYPAPL 812
            SL +L L +EFD  ++PFYLSF L  VS       E+ +HR  DFC+PVG  +G+P PL
Sbjct: 176 SSLSELMLGLEFDGKESPFYLSFHLKLVSGRDPDGREMLTHRDTDFCIPVGFTAGHPLPL 235

Query: 813 GVSFSDD 833
           G+S   D
Sbjct: 236 GLSSGPD 242