BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Akebia24_contig00007094
         (1987 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AFG26324.1| 4-coumarate-CoA ligase [Cinnamomum osmophloeum]        913   0.0  
gb|AHL44980.1| 4-coumarate:coenzyme A ligase 4 [Fraxinus mandshu...   886   0.0  
ref|XP_002520028.1| AMP dependent CoA ligase, putative [Ricinus ...   883   0.0  
ref|XP_007029575.1| 4-coumarate:CoA ligase 3 [Theobroma cacao] g...   880   0.0  
gb|AFC89538.1| 4-coumarate: coenzyme A ligase 2 [Populus tomentosa]   865   0.0  
gb|ACT32027.1| 4-coumarate:CoA ligase 1 [Gossypium hirsutum]          863   0.0  
ref|XP_002274994.1| PREDICTED: 4-coumarate--CoA ligase 2 [Vitis ...   863   0.0  
gb|AEX32786.1| coumaroyl-CoA ligase [Vitis vinifera]                  862   0.0  
gb|ACZ06243.1| 4-coumarate:CoA ligase 2 [Gossypium hirsutum]          860   0.0  
ref|XP_002325815.1| 4-coumarate-CoA ligase family protein [Popul...   860   0.0  
gb|ADU15550.1| 4CL [Gossypium hirsutum]                               859   0.0  
gb|AAC24504.1| 4-coumarate:CoA ligase [Populus tremuloides]           854   0.0  
ref|XP_006443197.1| hypothetical protein CICLE_v10019532mg [Citr...   853   0.0  
gb|AAK58909.1| 4-coumarate:CoA ligase 4 [Populus trichocarpa x P...   851   0.0  
ref|XP_004307864.1| PREDICTED: LOW QUALITY PROTEIN: 4-coumarate-...   850   0.0  
gb|AFG30056.1| 4-coumarate: CoA ligase [Malus hybrid cultivar]        848   0.0  
gb|ADE96997.1| p-coumarate:CoA-ligase 3 [Sorbus aucuparia]            847   0.0  
gb|EYU45376.1| hypothetical protein MIMGU_mgv1a003734mg [Mimulus...   847   0.0  
gb|AGW16013.1| 4-coumarate:coenzyme A ligase 1 [Pueraria montana...   843   0.0  
gb|ADZ54779.1| 4-coumarate-CoA ligase [Prunus avium]                  843   0.0  

>gb|AFG26324.1| 4-coumarate-CoA ligase [Cinnamomum osmophloeum]
          Length = 565

 Score =  913 bits (2359), Expect = 0.0
 Identities = 457/563 (81%), Positives = 501/563 (88%), Gaps = 1/563 (0%)
 Frame = -3

Query: 1856 MISIVSPETTPHEVSISEI-ENPSSETFIFRSKLPDIPISNHLPLHTYCFEKVSEFAEKP 1680
            M+S+ SPET P   S +   ENP  E FIFRSKLPDIPISNHLPLHTYCFEK++EF++ P
Sbjct: 1    MLSVASPETQPQIPSPTPTPENPPVENFIFRSKLPDIPISNHLPLHTYCFEKITEFSDAP 60

Query: 1679 CLISGSSGKIYTFAETHLICQKTAVGLSKLGIKKGDVIMVLLQNCPEFVFSFMGASMIGA 1500
            CLISGSSG+IYTFAETHLICQK+A GLSKLGIKKGDVIM+LLQN PEF FSFMGASMIGA
Sbjct: 61   CLISGSSGRIYTFAETHLICQKSAAGLSKLGIKKGDVIMILLQNSPEFAFSFMGASMIGA 120

Query: 1499 ITTTANPFYTSTEIFKQLSASNAKLIVTQSQYVDKLRDSTENSPKIGEDFNVVTIDDPPE 1320
            ITTTANPF TS EIFKQ S S AKL++TQSQYVDKLRD+ E  PKIGEDF V+T+DDPPE
Sbjct: 121  ITTTANPFCTSAEIFKQFSVSGAKLVITQSQYVDKLRDTGEKFPKIGEDFTVITVDDPPE 180

Query: 1319 NCHHFSVISNADEKEIGSVSIHPDDPVALPFSSGTTGLPKGVILTHKSLISSVAQQVDGE 1140
            +C HFSVIS ADE+EI SVSI PDDPV++PFSSGTTGLPKGV LTHKSLISSVAQQVDGE
Sbjct: 181  SCLHFSVISGADEREIPSVSIDPDDPVSMPFSSGTTGLPKGVFLTHKSLISSVAQQVDGE 240

Query: 1139 NPNLYLKSDDIVLCVLPLFHIFSLNSVLLCSLRTGSGVMLLQKFEIGAFLELIQKYRVSX 960
            NPNLYLK+DDIVLCVLPLFHI+SLNSVLLCSLR G+GV+L+ KFEI + LELIQ++RVS 
Sbjct: 241  NPNLYLKTDDIVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMHKFEIRSLLELIQRHRVSV 300

Query: 959  XXXXXXXXXXXAKNPMVESFDLSSIRIVLSGAAPLGKELEDALKSRVPQAIFGQGYGMTE 780
                       AKNPMV SFDLSSIRIVLSGAAPLGKELE+AL SR+PQAIFGQGYGMTE
Sbjct: 301  APVVPPLVLALAKNPMVASFDLSSIRIVLSGAAPLGKELEEALHSRLPQAIFGQGYGMTE 360

Query: 779  AGPVLSMCSAFAKQPFPTKSGSCGTVVRNAELKVVDPETGLSLRHNQPGEICIRGPQIMK 600
            AGPVLSMC  FA+Q FPTKSGSCGTVVRNAELKV+DPETG SL++NQPGEICIRGPQIMK
Sbjct: 361  AGPVLSMCLGFARQAFPTKSGSCGTVVRNAELKVIDPETGFSLQYNQPGEICIRGPQIMK 420

Query: 599  GYLNDPDASKSTIDVEGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELEALLV 420
            GYLND +A+ STIDV+GWLHTGD+GYVDDD+EVFIVDRVKELIKFKGFQVPPAELEALL+
Sbjct: 421  GYLNDAEATASTIDVDGWLHTGDVGYVDDDDEVFIVDRVKELIKFKGFQVPPAELEALLI 480

Query: 419  SHPSIADAAVVPQKDVMAGEVPVAFVVPSKDFEITEEAVKDFIAKQVVFYKRLHKVYFIH 240
            SHPSIADAAVVPQKD  AGEVPVAFVV S  FE+TEEAVK+FIAKQVVFYKRLHKVYF+H
Sbjct: 481  SHPSIADAAVVPQKDEAAGEVPVAFVVRSSGFELTEEAVKEFIAKQVVFYKRLHKVYFVH 540

Query: 239  AIPKSPAGKILRKDLRAKLASSS 171
            AIPKS +GKIL KDLRAKLA+ S
Sbjct: 541  AIPKSASGKILGKDLRAKLATGS 563


>gb|AHL44980.1| 4-coumarate:coenzyme A ligase 4 [Fraxinus mandshurica]
          Length = 573

 Score =  886 bits (2289), Expect = 0.0
 Identities = 440/567 (77%), Positives = 503/567 (88%), Gaps = 5/567 (0%)
 Frame = -3

Query: 1856 MISIVSPETTPHEVS-ISEIENP----SSETFIFRSKLPDIPISNHLPLHTYCFEKVSEF 1692
            M+S+ S ET   E+S ++  + P    S ET +F SKLP IPISNHLPLHTYCF  ++++
Sbjct: 1    MLSVASVETQNSELSSLASPQKPQTQSSEETHVFISKLPQIPISNHLPLHTYCFGNLAQY 60

Query: 1691 AEKPCLISGSSGKIYTFAETHLICQKTAVGLSKLGIKKGDVIMVLLQNCPEFVFSFMGAS 1512
             E+PCLI GS+GK Y+F+ETHL+C+KTA GLSKLG+KKGDVIMVLLQNCPEFVF+FMGAS
Sbjct: 61   PERPCLIVGSTGKTYSFSETHLLCRKTAAGLSKLGLKKGDVIMVLLQNCPEFVFTFMGAS 120

Query: 1511 MIGAITTTANPFYTSTEIFKQLSASNAKLIVTQSQYVDKLRDSTENSPKIGEDFNVVTID 1332
            MIGA+TTTANPFYTS+E+FKQ  AS AK+I+TQSQYVDKLRD  +N+PK+GEDF V+TID
Sbjct: 121  MIGAVTTTANPFYTSSEVFKQFGASEAKMIITQSQYVDKLRDPGQNNPKLGEDFTVITID 180

Query: 1331 DPPENCHHFSVISNADEKEIGSVSIHPDDPVALPFSSGTTGLPKGVILTHKSLISSVAQQ 1152
            DPPENC HFSV+S A+E +I SVSI P+DPVALPFSSGTTGLPKGVILTHKSLI+SVAQQ
Sbjct: 181  DPPENCLHFSVLSEANENDIPSVSIDPNDPVALPFSSGTTGLPKGVILTHKSLITSVAQQ 240

Query: 1151 VDGENPNLYLKSDDIVLCVLPLFHIFSLNSVLLCSLRTGSGVMLLQKFEIGAFLELIQKY 972
            VDGENPNLYLKSDD+VLCVLPLFHI+SLNSVLLCSLR G+GV+L+QKFEIG+FLELIQ++
Sbjct: 241  VDGENPNLYLKSDDVVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMQKFEIGSFLELIQRH 300

Query: 971  RVSXXXXXXXXXXXXAKNPMVESFDLSSIRIVLSGAAPLGKELEDALKSRVPQAIFGQGY 792
            RVS            AKNP+V+ FDLSSIR+VLSGAAPLGKELE AL++RVPQAIFGQGY
Sbjct: 301  RVSVAAVVPPLVLALAKNPLVDKFDLSSIRVVLSGAAPLGKELEAALRNRVPQAIFGQGY 360

Query: 791  GMTEAGPVLSMCSAFAKQPFPTKSGSCGTVVRNAELKVVDPETGLSLRHNQPGEICIRGP 612
            GMTEAGPVLSM +AFAKQP  TKSGSCG VVRNAELKVVDPE+G SL  NQPGEICIRGP
Sbjct: 361  GMTEAGPVLSMSTAFAKQPILTKSGSCGNVVRNAELKVVDPESGSSLPRNQPGEICIRGP 420

Query: 611  QIMKGYLNDPDASKSTIDVEGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELE 432
            QIMKGYLND +A+ +TIDV+GWLHTGDIGYVDDD+++FIVDRVKELIKFKGFQVPPAELE
Sbjct: 421  QIMKGYLNDDEATANTIDVDGWLHTGDIGYVDDDDDIFIVDRVKELIKFKGFQVPPAELE 480

Query: 431  ALLVSHPSIADAAVVPQKDVMAGEVPVAFVVPSKDFEITEEAVKDFIAKQVVFYKRLHKV 252
            ALLVSHP+IADAAVVPQKD  AGEVPVAFVV S  FE++EEAVK+FIAKQVVFYK+LHKV
Sbjct: 481  ALLVSHPNIADAAVVPQKDEAAGEVPVAFVVRSNGFELSEEAVKEFIAKQVVFYKKLHKV 540

Query: 251  YFIHAIPKSPAGKILRKDLRAKLASSS 171
            YFIHAIPKSPAGKILRKDLRAKL +++
Sbjct: 541  YFIHAIPKSPAGKILRKDLRAKLVTTT 567


>ref|XP_002520028.1| AMP dependent CoA ligase, putative [Ricinus communis]
            gi|223540792|gb|EEF42352.1| AMP dependent CoA ligase,
            putative [Ricinus communis]
          Length = 573

 Score =  883 bits (2282), Expect = 0.0
 Identities = 447/570 (78%), Positives = 498/570 (87%), Gaps = 8/570 (1%)
 Frame = -3

Query: 1856 MISIVSPETTPHEVSIS---EIENPSS---ETFIFRSKLPDIPISNHLPLHTYCFEKVSE 1695
            MISI S E    E+S S   + +NPSS   ET IFRSKLPDIPISNHLPLHTYCFE + +
Sbjct: 1    MISIASLEPPKQELSSSSSSQKQNPSSSSSETHIFRSKLPDIPISNHLPLHTYCFENLFK 60

Query: 1694 FAEKPCLISGSSGKIYTFAETHLICQKTAVGLSKLGIKKGDVIMVLLQNCPEFVFSFMGA 1515
             ++KPCLISGS+GK Y+FAETHLI QKTA GLS LGIKKGDVIM+LLQNCPEFVFSFMGA
Sbjct: 61   LSDKPCLISGSTGKTYSFAETHLISQKTAAGLSSLGIKKGDVIMILLQNCPEFVFSFMGA 120

Query: 1514 SMIGAITTTANPFYTSTEIFKQLSASNAKLIVTQSQYVDKLRDSTEN-SPKIGEDFNVVT 1338
            SMIGA+ TTANPFYTS EIFKQL++S  KLI+TQS YVDKLRD      PK+GEDF V+T
Sbjct: 121  SMIGAVATTANPFYTSNEIFKQLNSSRPKLIITQSLYVDKLRDPDHKIQPKLGEDFTVIT 180

Query: 1337 IDDPPENCHHFSVISNADEKEIGSV-SIHPDDPVALPFSSGTTGLPKGVILTHKSLISSV 1161
            ID+PPENC HF+V+S A E EI  V + +PDDPVALPFSSGTTGLPKGVILTHKSLI+SV
Sbjct: 181  IDEPPENCLHFTVLSEASETEIPEVINANPDDPVALPFSSGTTGLPKGVILTHKSLITSV 240

Query: 1160 AQQVDGENPNLYLKSDDIVLCVLPLFHIFSLNSVLLCSLRTGSGVMLLQKFEIGAFLELI 981
            AQQVDGENPNLYLK DD+VLCVLPLFHI+SLNSVLLCSLR G+ V+L+QKF+IGA LELI
Sbjct: 241  AQQVDGENPNLYLKEDDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLLMQKFQIGALLELI 300

Query: 980  QKYRVSXXXXXXXXXXXXAKNPMVESFDLSSIRIVLSGAAPLGKELEDALKSRVPQAIFG 801
            QK++VS            AKNPMV  FDLSSIR+VLSGAAPLGKELEDAL+SRVPQAI G
Sbjct: 301  QKHKVSVAAVVPPLVLALAKNPMVAEFDLSSIRVVLSGAAPLGKELEDALRSRVPQAILG 360

Query: 800  QGYGMTEAGPVLSMCSAFAKQPFPTKSGSCGTVVRNAELKVVDPETGLSLRHNQPGEICI 621
            QGYGMTEAGPVLSMC  FAKQPFPTKSGSCGTVVRNAELKV+DPETG SL +NQPGEICI
Sbjct: 361  QGYGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICI 420

Query: 620  RGPQIMKGYLNDPDASKSTIDVEGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPA 441
            RGPQIMKGYLNDP+A+ +TIDVEGWLHTGDIGYVDDD+E+FIVDRVKE+IKFKGFQVPPA
Sbjct: 421  RGPQIMKGYLNDPEATANTIDVEGWLHTGDIGYVDDDDEIFIVDRVKEIIKFKGFQVPPA 480

Query: 440  ELEALLVSHPSIADAAVVPQKDVMAGEVPVAFVVPSKDFEITEEAVKDFIAKQVVFYKRL 261
            ELEALL++HPSIADAAVVPQKD +AGEVPVAFVV S  FE++EE VK++IAKQVVFYK+L
Sbjct: 481  ELEALLLNHPSIADAAVVPQKDEVAGEVPVAFVVRSNGFELSEEDVKEYIAKQVVFYKKL 540

Query: 260  HKVYFIHAIPKSPAGKILRKDLRAKLASSS 171
            HKVYFIHAIPKSP+GKILRKDL+AKLA+ S
Sbjct: 541  HKVYFIHAIPKSPSGKILRKDLKAKLATPS 570


>ref|XP_007029575.1| 4-coumarate:CoA ligase 3 [Theobroma cacao]
            gi|508718180|gb|EOY10077.1| 4-coumarate:CoA ligase 3
            [Theobroma cacao]
          Length = 575

 Score =  880 bits (2273), Expect = 0.0
 Identities = 445/571 (77%), Positives = 497/571 (87%), Gaps = 9/571 (1%)
 Frame = -3

Query: 1856 MISIVSP-ETTPHEVSIS---EIENPSSET---FIFRSKLPDIPISNHLPLHTYCFEKVS 1698
            MI+I  P E    E+S S       PSSET    IFRSKLPDIPISNHLPLHTYCFE +S
Sbjct: 1    MITIADPVEPQKPELSPSLSPPATKPSSETTGDHIFRSKLPDIPISNHLPLHTYCFENLS 60

Query: 1697 EFAEKPCLISGSSGKIYTFAETHLICQKTAVGLSKLGIKKGDVIMVLLQNCPEFVFSFMG 1518
             F +KPCLISGSSGK Y+F+ETHLI QKTA GLS LGI+KGDVIM+LLQNC EFVFSFMG
Sbjct: 61   SFPDKPCLISGSSGKTYSFSETHLIAQKTAAGLSNLGIRKGDVIMILLQNCAEFVFSFMG 120

Query: 1517 ASMIGAITTTANPFYTSTEIFKQLSASNAKLIVTQSQYVDKLRDST--ENSPKIGEDFNV 1344
            ASMIGA++TTANPFYTSTEIFKQ   + AKLIVTQSQYVDKL+D++  EN PK+GEDF V
Sbjct: 121  ASMIGAVSTTANPFYTSTEIFKQYKVAQAKLIVTQSQYVDKLKDTSNNENFPKMGEDFKV 180

Query: 1343 VTIDDPPENCHHFSVISNADEKEIGSVSIHPDDPVALPFSSGTTGLPKGVILTHKSLISS 1164
            +TIDDPPENC HF+V+S A+E EI  VSI PDDPVALPFSSGTTGLPKGV+LTHKSLI+S
Sbjct: 181  ITIDDPPENCLHFTVLSEANENEIPQVSIDPDDPVALPFSSGTTGLPKGVVLTHKSLITS 240

Query: 1163 VAQQVDGENPNLYLKSDDIVLCVLPLFHIFSLNSVLLCSLRTGSGVMLLQKFEIGAFLEL 984
            VAQQVDGENPNLYLK DD+VLCVLPLFHI+SLNSVLLC+LR G+ V+L+QKFEIGA LEL
Sbjct: 241  VAQQVDGENPNLYLKHDDVVLCVLPLFHIYSLNSVLLCALRAGAAVLLMQKFEIGALLEL 300

Query: 983  IQKYRVSXXXXXXXXXXXXAKNPMVESFDLSSIRIVLSGAAPLGKELEDALKSRVPQAIF 804
            IQK++VS            AKNPMV  +DLSSIR+VLSGAAPLGKELE+AL+ RVPQA+ 
Sbjct: 301  IQKHKVSVAAVVPPLVLALAKNPMVAQYDLSSIRVVLSGAAPLGKELEEALRGRVPQAVL 360

Query: 803  GQGYGMTEAGPVLSMCSAFAKQPFPTKSGSCGTVVRNAELKVVDPETGLSLRHNQPGEIC 624
            GQGYGMTEAGPVLSMC  FAKQPFPTKSGSCGTVVRNAELKV+DPETG SL +NQPGEIC
Sbjct: 361  GQGYGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEIC 420

Query: 623  IRGPQIMKGYLNDPDASKSTIDVEGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPP 444
            IRG QIMKGYLND  A+ +TIDVEGWLHTGDIGYVDD +E+FIVDRVKE+IKFKGFQVPP
Sbjct: 421  IRGSQIMKGYLNDTSATATTIDVEGWLHTGDIGYVDDGDEIFIVDRVKEIIKFKGFQVPP 480

Query: 443  AELEALLVSHPSIADAAVVPQKDVMAGEVPVAFVVPSKDFEITEEAVKDFIAKQVVFYKR 264
            AELE+LLVSHPSIADAAVVPQKD +AGEVPVAFVV S  FE+TEEAVK+FIAKQVVFYK+
Sbjct: 481  AELESLLVSHPSIADAAVVPQKDEVAGEVPVAFVVRSNGFELTEEAVKEFIAKQVVFYKK 540

Query: 263  LHKVYFIHAIPKSPAGKILRKDLRAKLASSS 171
            LHKV+F+HAIPKSP+GKILRKDLRAKLA+++
Sbjct: 541  LHKVHFVHAIPKSPSGKILRKDLRAKLAAAT 571


>gb|AFC89538.1| 4-coumarate: coenzyme A ligase 2 [Populus tomentosa]
          Length = 569

 Score =  865 bits (2234), Expect = 0.0
 Identities = 437/566 (77%), Positives = 495/566 (87%), Gaps = 5/566 (0%)
 Frame = -3

Query: 1853 ISIVSPETTPHEVSISEIEN-PSSET--FIFRSKLPDIPISNHLPLHTYCFEKVSEFAEK 1683
            +S+ + E    E+S  + +N PSS     IFRSKLPDI ISNHLPLH YCFE +S+F+++
Sbjct: 1    MSVATVEPPKPELSPPQNQNAPSSHETDHIFRSKLPDITISNHLPLHAYCFENLSDFSDR 60

Query: 1682 PCLISGSSGKIYTFAETHLICQKTAVGLSKLGIKKGDVIMVLLQNCPEFVFSFMGASMIG 1503
            PCLISGS+GK Y+FAETHLI +K A GLS LGIKKGDVIM LLQNCPEFVFSFMGASMIG
Sbjct: 61   PCLISGSTGKTYSFAETHLISRKVAAGLSNLGIKKGDVIMTLLQNCPEFVFSFMGASMIG 120

Query: 1502 AITTTANPFYTSTEIFKQLSASNAKLIVTQSQYVDKLRDST--ENSPKIGEDFNVVTIDD 1329
            A+TTTANPFYT +EIFKQ SAS AKLI+TQSQYV+KL DS   EN+ K GEDF V+TIDD
Sbjct: 121  AVTTTANPFYTQSEIFKQFSASRAKLIITQSQYVNKLGDSDCHENNQKPGEDFIVITIDD 180

Query: 1328 PPENCHHFSVISNADEKEIGSVSIHPDDPVALPFSSGTTGLPKGVILTHKSLISSVAQQV 1149
            PPENC HF+V+  A+E E+ ++SI PDDPVALPFSSGTTGLPKGVILTHKSLI+SVAQQV
Sbjct: 181  PPENCRHFNVLVEANESEMPTISILPDDPVALPFSSGTTGLPKGVILTHKSLITSVAQQV 240

Query: 1148 DGENPNLYLKSDDIVLCVLPLFHIFSLNSVLLCSLRTGSGVMLLQKFEIGAFLELIQKYR 969
            DGE PNLYLK DD+VLCVLPLFHIFSLNSVLLCSLR GS V+L+QKFEIG+ LELIQK+ 
Sbjct: 241  DGEIPNLYLKQDDVVLCVLPLFHIFSLNSVLLCSLRAGSAVLLMQKFEIGSLLELIQKHN 300

Query: 968  VSXXXXXXXXXXXXAKNPMVESFDLSSIRIVLSGAAPLGKELEDALKSRVPQAIFGQGYG 789
            VS            AKNP+V +FDLSSIR+VLSGAAPLGKELEDAL+SRVPQAI GQGYG
Sbjct: 301  VSVAAVVPPLVLALAKNPLVANFDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGYG 360

Query: 788  MTEAGPVLSMCSAFAKQPFPTKSGSCGTVVRNAELKVVDPETGLSLRHNQPGEICIRGPQ 609
            MTEAGPVLSMC AF+KQPFPTKSGSCGTVVRNAELKV+DPETG SL +NQPGEICIRG Q
Sbjct: 361  MTEAGPVLSMCLAFSKQPFPTKSGSCGTVVRNAELKVIDPETGGSLGYNQPGEICIRGSQ 420

Query: 608  IMKGYLNDPDASKSTIDVEGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELEA 429
            IMKGYLND +A+ +TIDVEGWLHTGDIGYVDDD+E+FIVDRVKE+IKFKGFQVPPAELEA
Sbjct: 421  IMKGYLNDAEATANTIDVEGWLHTGDIGYVDDDDEIFIVDRVKEIIKFKGFQVPPAELEA 480

Query: 428  LLVSHPSIADAAVVPQKDVMAGEVPVAFVVPSKDFEITEEAVKDFIAKQVVFYKRLHKVY 249
            LLV+HPSIADAAVVPQKD +AGEVPVAFVV S D +++EEAVKD+IAKQVVFYK+LHKV+
Sbjct: 481  LLVNHPSIADAAVVPQKDEVAGEVPVAFVVRSDDLDLSEEAVKDYIAKQVVFYKKLHKVF 540

Query: 248  FIHAIPKSPAGKILRKDLRAKLASSS 171
            F+H+IPKS +GKILRKDLRAKLA+S+
Sbjct: 541  FVHSIPKSASGKILRKDLRAKLATST 566


>gb|ACT32027.1| 4-coumarate:CoA ligase 1 [Gossypium hirsutum]
          Length = 573

 Score =  863 bits (2231), Expect = 0.0
 Identities = 431/567 (76%), Positives = 489/567 (86%), Gaps = 8/567 (1%)
 Frame = -3

Query: 1847 IVSPETTPHEVSISEIENPSSET---FIFRSKLPDIPISNHLPLHTYCFEKVSEFAEKPC 1677
            ++S    P E+   + +  S ET    IFRSKLPDIPISNHLPLHTYCFE +   ++KPC
Sbjct: 1    MISIADHPVELESPKPDQLSPETPADHIFRSKLPDIPISNHLPLHTYCFENLPSLSDKPC 60

Query: 1676 LISGSSGKIYTFAETHLICQKTAVGLSKLGIKKGDVIMVLLQNCPEFVFSFMGASMIGAI 1497
            LISGSSGKIY+F+ETHLI QKTA GLS LGI+KGD IM+LL NC EFVF+FMGASMIGA+
Sbjct: 61   LISGSSGKIYSFSETHLIAQKTAAGLSNLGIQKGDAIMILLHNCAEFVFAFMGASMIGAV 120

Query: 1496 TTTANPFYTSTEIFKQLSASNAKLIVTQSQYVDKLRDST-----ENSPKIGEDFNVVTID 1332
            +TTANPFYTS EIFKQ  A+ AKLI+TQSQYVDKL+D+      EN PKIGEDF V+TID
Sbjct: 121  STTANPFYTSKEIFKQFKAARAKLIITQSQYVDKLKDTNNNDGDENFPKIGEDFKVITID 180

Query: 1331 DPPENCHHFSVISNADEKEIGSVSIHPDDPVALPFSSGTTGLPKGVILTHKSLISSVAQQ 1152
            DPPENC  FS++S A+E EI  V+IHPDDPVALPFSSGTTGLPKGV+LTHKSLI+SVAQQ
Sbjct: 181  DPPENCLDFSMLSEANENEIPQVTIHPDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQ 240

Query: 1151 VDGENPNLYLKSDDIVLCVLPLFHIFSLNSVLLCSLRTGSGVMLLQKFEIGAFLELIQKY 972
            VDGENPNLYL  DD+VLCVLPLFHI+SLNSVLLCSLR G+ V+L+QKFEIG  LELIQ++
Sbjct: 241  VDGENPNLYLTHDDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLLMQKFEIGTLLELIQRH 300

Query: 971  RVSXXXXXXXXXXXXAKNPMVESFDLSSIRIVLSGAAPLGKELEDALKSRVPQAIFGQGY 792
            +VS            AKNP+V  FDLSSIR+VLSGAAPLGKELEDAL+ RVPQA+ GQGY
Sbjct: 301  KVSVAAVVPPLVVLLAKNPVVAQFDLSSIRVVLSGAAPLGKELEDALRGRVPQAVLGQGY 360

Query: 791  GMTEAGPVLSMCSAFAKQPFPTKSGSCGTVVRNAELKVVDPETGLSLRHNQPGEICIRGP 612
            GMTEAGPVLSMC  FAKQPFPTKSGSCGTVVRNAELKV+DPETG SL +NQPGEICIRG 
Sbjct: 361  GMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIRGS 420

Query: 611  QIMKGYLNDPDASKSTIDVEGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELE 432
            QIMKGYLND  A+ +TIDVEGWLHTGDIGYVD+D+E+FIVDRVKE+IKFKGFQVPPAELE
Sbjct: 421  QIMKGYLNDGVATAATIDVEGWLHTGDIGYVDEDDEIFIVDRVKEIIKFKGFQVPPAELE 480

Query: 431  ALLVSHPSIADAAVVPQKDVMAGEVPVAFVVPSKDFEITEEAVKDFIAKQVVFYKRLHKV 252
            +LL+SHPSIADAAVVPQKD +AGEVPVAFVV S  FE+TEEAVK+FIAKQVV+YKRLHKV
Sbjct: 481  SLLISHPSIADAAVVPQKDEVAGEVPVAFVVRSNGFELTEEAVKEFIAKQVVYYKRLHKV 540

Query: 251  YFIHAIPKSPAGKILRKDLRAKLASSS 171
            +F+HAIPKSP+GKILRKDLRAKLA+++
Sbjct: 541  HFVHAIPKSPSGKILRKDLRAKLAAAA 567


>ref|XP_002274994.1| PREDICTED: 4-coumarate--CoA ligase 2 [Vitis vinifera]
          Length = 570

 Score =  863 bits (2231), Expect = 0.0
 Identities = 427/536 (79%), Positives = 475/536 (88%), Gaps = 1/536 (0%)
 Frame = -3

Query: 1775 IFRSKLPDIPISNHLPLHTYCFEKVSEFAEKPCLISGSSGKIYTFAETHLICQKTAVGLS 1596
            +FRSKLP+IPISNHLPLHTYCFE  S+FA++PCLI GS+ K Y+FAETHLI +K   G +
Sbjct: 31   VFRSKLPEIPISNHLPLHTYCFENYSQFADRPCLIVGSTNKTYSFAETHLISRKVGAGFA 90

Query: 1595 KLGIKKGDVIMVLLQNCPEFVFSFMGASMIGAITTTANPFYTSTEIFKQLSASNAKLIVT 1416
             LG+K+GDV+M+LLQNC EF FSF+GASM+GA+TTTANPFYTS EIFKQL+AS AK++VT
Sbjct: 91   HLGLKQGDVVMILLQNCAEFAFSFLGASMVGAVTTTANPFYTSAEIFKQLNASKAKIVVT 150

Query: 1415 QSQYVDKLRDSTENS-PKIGEDFNVVTIDDPPENCHHFSVISNADEKEIGSVSIHPDDPV 1239
            Q+QYVDKLRD  +    KIGE F V+TIDDPPENC HFSV+S A+E E+  VSI+ DDPV
Sbjct: 151  QAQYVDKLRDYPDGQVAKIGEGFTVITIDDPPENCMHFSVVSEANESELPEVSINSDDPV 210

Query: 1238 ALPFSSGTTGLPKGVILTHKSLISSVAQQVDGENPNLYLKSDDIVLCVLPLFHIFSLNSV 1059
            ALPFSSGTTGLPKGV+LTHKSLI+SVAQQVDGENPNL+L  DD+VLCVLPLFHI+SLNSV
Sbjct: 211  ALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLHLTPDDVVLCVLPLFHIYSLNSV 270

Query: 1058 LLCSLRTGSGVMLLQKFEIGAFLELIQKYRVSXXXXXXXXXXXXAKNPMVESFDLSSIRI 879
            LLCSLR G+ V+L+QKFEIG  LELIQ+YRVS            AKNPMVESFDLSSIR+
Sbjct: 271  LLCSLRAGAAVLLMQKFEIGTLLELIQRYRVSVAAVVPPLVLALAKNPMVESFDLSSIRV 330

Query: 878  VLSGAAPLGKELEDALKSRVPQAIFGQGYGMTEAGPVLSMCSAFAKQPFPTKSGSCGTVV 699
            VLSGAAPLGKELE AL+SRVPQA+ GQGYGMTEAGPVLSMC AFAKQPFPTKSGSCGTVV
Sbjct: 331  VLSGAAPLGKELEAALRSRVPQAVLGQGYGMTEAGPVLSMCLAFAKQPFPTKSGSCGTVV 390

Query: 698  RNAELKVVDPETGLSLRHNQPGEICIRGPQIMKGYLNDPDASKSTIDVEGWLHTGDIGYV 519
            RNAELKVVDPETG SL  NQPGEICIRG QIMKGYLNDP+A+ STIDV+GWLHTGDIGYV
Sbjct: 391  RNAELKVVDPETGCSLGRNQPGEICIRGQQIMKGYLNDPEATASTIDVDGWLHTGDIGYV 450

Query: 518  DDDEEVFIVDRVKELIKFKGFQVPPAELEALLVSHPSIADAAVVPQKDVMAGEVPVAFVV 339
            DDDEEVFIVDRVKELIKFKGFQVPPAELEALLVSHPSIADAAVVPQKD +AGEVPVAFVV
Sbjct: 451  DDDEEVFIVDRVKELIKFKGFQVPPAELEALLVSHPSIADAAVVPQKDDVAGEVPVAFVV 510

Query: 338  PSKDFEITEEAVKDFIAKQVVFYKRLHKVYFIHAIPKSPAGKILRKDLRAKLASSS 171
             S  FE+TEEAVK+FI+KQVVFYKRLHKVYF+HAIPKSP+GKILRKDLRAKLA  +
Sbjct: 511  RSNGFELTEEAVKEFISKQVVFYKRLHKVYFVHAIPKSPSGKILRKDLRAKLAEKT 566


>gb|AEX32786.1| coumaroyl-CoA ligase [Vitis vinifera]
          Length = 570

 Score =  862 bits (2227), Expect = 0.0
 Identities = 426/536 (79%), Positives = 474/536 (88%), Gaps = 1/536 (0%)
 Frame = -3

Query: 1775 IFRSKLPDIPISNHLPLHTYCFEKVSEFAEKPCLISGSSGKIYTFAETHLICQKTAVGLS 1596
            +FRSKLP+IPISNHLPLHTYCFE  S+FA++PCLI GS+ K Y+FAETHLI +K   G +
Sbjct: 31   VFRSKLPEIPISNHLPLHTYCFENYSQFADRPCLIVGSTNKTYSFAETHLISRKVGAGFA 90

Query: 1595 KLGIKKGDVIMVLLQNCPEFVFSFMGASMIGAITTTANPFYTSTEIFKQLSASNAKLIVT 1416
             LG+K+GDV+M+LLQNC EF FSF+GASM+GA+TTTANPFYTS EIFKQL+AS AK++VT
Sbjct: 91   HLGLKQGDVVMILLQNCAEFAFSFLGASMVGAVTTTANPFYTSAEIFKQLNASKAKIVVT 150

Query: 1415 QSQYVDKLRDSTENS-PKIGEDFNVVTIDDPPENCHHFSVISNADEKEIGSVSIHPDDPV 1239
            Q+QYVDKLRD  +    KIGE F V+TIDDPPENC HFSV+S A+E E+  VSI+ DDPV
Sbjct: 151  QAQYVDKLRDYPDGQVAKIGEGFTVITIDDPPENCMHFSVVSEANESELPEVSINSDDPV 210

Query: 1238 ALPFSSGTTGLPKGVILTHKSLISSVAQQVDGENPNLYLKSDDIVLCVLPLFHIFSLNSV 1059
            ALPFSSGTTGLPKGV+LTHKSLI+SVAQQVDGENPNL+L  DD+VLCVLPLFHI+SLNSV
Sbjct: 211  ALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNLHLTPDDVVLCVLPLFHIYSLNSV 270

Query: 1058 LLCSLRTGSGVMLLQKFEIGAFLELIQKYRVSXXXXXXXXXXXXAKNPMVESFDLSSIRI 879
            LLCSLR G+ V+L+QKFEIG  LELIQ+YRVS            AKNPMVESFDLSSIR+
Sbjct: 271  LLCSLRAGAAVLLMQKFEIGTLLELIQRYRVSVAAVVPPLVLALAKNPMVESFDLSSIRV 330

Query: 878  VLSGAAPLGKELEDALKSRVPQAIFGQGYGMTEAGPVLSMCSAFAKQPFPTKSGSCGTVV 699
            VLSGAAPLGKELE AL+SRVPQA+ GQGYGMTEAGPVLSMC  FAKQPFPTKSGSCGTVV
Sbjct: 331  VLSGAAPLGKELEAALRSRVPQAVLGQGYGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVV 390

Query: 698  RNAELKVVDPETGLSLRHNQPGEICIRGPQIMKGYLNDPDASKSTIDVEGWLHTGDIGYV 519
            RNAELKVVDPETG SL  NQPGEICIRG QIMKGYLNDP+A+ STIDV+GWLHTGDIGYV
Sbjct: 391  RNAELKVVDPETGCSLGRNQPGEICIRGQQIMKGYLNDPEATASTIDVDGWLHTGDIGYV 450

Query: 518  DDDEEVFIVDRVKELIKFKGFQVPPAELEALLVSHPSIADAAVVPQKDVMAGEVPVAFVV 339
            DDDEEVFIVDRVKELIKFKGFQVPPAELEALLVSHPSIADAAVVPQKD +AGEVPVAFVV
Sbjct: 451  DDDEEVFIVDRVKELIKFKGFQVPPAELEALLVSHPSIADAAVVPQKDDVAGEVPVAFVV 510

Query: 338  PSKDFEITEEAVKDFIAKQVVFYKRLHKVYFIHAIPKSPAGKILRKDLRAKLASSS 171
             S  FE+TEEAVK+FI+KQVVFYKRLHKVYF+HAIPKSP+GKILRKDLRAKLA  +
Sbjct: 511  RSNGFELTEEAVKEFISKQVVFYKRLHKVYFVHAIPKSPSGKILRKDLRAKLAEKT 566


>gb|ACZ06243.1| 4-coumarate:CoA ligase 2 [Gossypium hirsutum]
          Length = 574

 Score =  860 bits (2223), Expect = 0.0
 Identities = 429/568 (75%), Positives = 489/568 (86%), Gaps = 9/568 (1%)
 Frame = -3

Query: 1847 IVSPETTPHEVSISEIENPSSET---FIFRSKLPDIPISNHLPLHTYCFEKVSEFAEKPC 1677
            ++S    P E+   + +  S ET    IFRSKLPDIPISNHLPLHTYCFE +   ++KPC
Sbjct: 1    MISIADHPVELESPKPDQLSPETPADHIFRSKLPDIPISNHLPLHTYCFENLPSLSDKPC 60

Query: 1676 LISGSSGKIYTFAETHLICQKTAVGLSKLGIKKGDVIMVLLQNCPEFVFSFMGASMIGAI 1497
            LISGSSGKIY+F+ETHLI QKTA GLSKLGI+KGD IM+LL NC EFVF+FMGASMIGA+
Sbjct: 61   LISGSSGKIYSFSETHLIAQKTAAGLSKLGIQKGDAIMILLHNCAEFVFAFMGASMIGAV 120

Query: 1496 TTTANPFYTSTEIFKQLSASNAKLIVTQSQYVDKLRDST------ENSPKIGEDFNVVTI 1335
            +TTANPFYTS EIFKQ  A+ AKLI+TQSQYVDKL+D+       EN PKIGEDF V+TI
Sbjct: 121  STTANPFYTSKEIFKQFKAARAKLIITQSQYVDKLKDTNNNNDGVENFPKIGEDFKVITI 180

Query: 1334 DDPPENCHHFSVISNADEKEIGSVSIHPDDPVALPFSSGTTGLPKGVILTHKSLISSVAQ 1155
            DDPPENC  FSV+S A+E EI  V+IHPDDPVALPFSSGTTGLPKGV+LTHKSLI+SVAQ
Sbjct: 181  DDPPENCLDFSVLSEANENEIPQVTIHPDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQ 240

Query: 1154 QVDGENPNLYLKSDDIVLCVLPLFHIFSLNSVLLCSLRTGSGVMLLQKFEIGAFLELIQK 975
            QVDGENPNLYL  +D+VLCVLPLFHI+SLNSVLLCSLR G+ V+L+QKFEIG  LELIQ+
Sbjct: 241  QVDGENPNLYLTHEDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLLMQKFEIGTLLELIQR 300

Query: 974  YRVSXXXXXXXXXXXXAKNPMVESFDLSSIRIVLSGAAPLGKELEDALKSRVPQAIFGQG 795
            ++VS            AKNP+V  FDLSSIR+VLSGAAPLGKELE+AL+ RVPQA+ GQG
Sbjct: 301  HKVSVAAVVPPLVLLLAKNPVVAQFDLSSIRVVLSGAAPLGKELEEALRGRVPQAVLGQG 360

Query: 794  YGMTEAGPVLSMCSAFAKQPFPTKSGSCGTVVRNAELKVVDPETGLSLRHNQPGEICIRG 615
            YGMTEAGPVLSMC  FAKQPFPTKSGSCGTVVRNAELKV+DPETG SL +NQPGEICIRG
Sbjct: 361  YGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIRG 420

Query: 614  PQIMKGYLNDPDASKSTIDVEGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAEL 435
             QIMKGYLND  A+ +T DVEGWLHTGDIGYVD+D+E+FIVDRVKE+IKFKGFQVPPAEL
Sbjct: 421  SQIMKGYLNDSVATAATTDVEGWLHTGDIGYVDEDDEIFIVDRVKEIIKFKGFQVPPAEL 480

Query: 434  EALLVSHPSIADAAVVPQKDVMAGEVPVAFVVPSKDFEITEEAVKDFIAKQVVFYKRLHK 255
            E+LL+SHPSIADAAVVPQKD +AGEVPVAFVV S  FE+TEEAVK+FIA+QVV+YKRLHK
Sbjct: 481  ESLLISHPSIADAAVVPQKDEVAGEVPVAFVVRSNGFELTEEAVKEFIARQVVYYKRLHK 540

Query: 254  VYFIHAIPKSPAGKILRKDLRAKLASSS 171
            V+F+HAIPKSP+GKILRKDLRAKLA+++
Sbjct: 541  VHFVHAIPKSPSGKILRKDLRAKLAAAA 568


>ref|XP_002325815.1| 4-coumarate-CoA ligase family protein [Populus trichocarpa]
            gi|118487236|gb|ABK95446.1| unknown [Populus trichocarpa]
            gi|222862690|gb|EEF00197.1| 4-coumarate-CoA ligase family
            protein [Populus trichocarpa]
          Length = 570

 Score =  860 bits (2221), Expect = 0.0
 Identities = 436/567 (76%), Positives = 491/567 (86%), Gaps = 5/567 (0%)
 Frame = -3

Query: 1856 MISIVSPETTPHEVSISEIEN-PSSET--FIFRSKLPDIPISNHLPLHTYCFEKVSEFAE 1686
            M+S+ + E    E+S  + +N PSS     IFRSKLPDI ISNHLPLH YCFE +S+F++
Sbjct: 1    MMSVATVEPPKPELSPPQNQNAPSSHETDHIFRSKLPDITISNHLPLHAYCFENLSDFSD 60

Query: 1685 KPCLISGSSGKIYTFAETHLICQKTAVGLSKLGIKKGDVIMVLLQNCPEFVFSFMGASMI 1506
            +PCLISGS+GK Y+FAETHLI +K A GLS LGIKKGDVIM LLQNCPEFVFSFMGASMI
Sbjct: 61   RPCLISGSTGKTYSFAETHLISRKVAAGLSNLGIKKGDVIMTLLQNCPEFVFSFMGASMI 120

Query: 1505 GAITTTANPFYTSTEIFKQLSASNAKLIVTQSQYVDKLRDST--ENSPKIGEDFNVVTID 1332
            GA+TTT NPFYT  EIFKQ SAS AKLI+TQSQ+V+KLRDS   EN+ K  EDF V+TID
Sbjct: 121  GAVTTTVNPFYTPGEIFKQFSASRAKLIITQSQHVNKLRDSDCHENNQKPEEDFIVITID 180

Query: 1331 DPPENCHHFSVISNADEKEIGSVSIHPDDPVALPFSSGTTGLPKGVILTHKSLISSVAQQ 1152
            DPPENC HF+V+  A+E E+ +VSIHPDDPVALPFSSGTTGLPKGVILTHKSLI+SVAQQ
Sbjct: 181  DPPENCLHFNVLVEANESEMPTVSIHPDDPVALPFSSGTTGLPKGVILTHKSLITSVAQQ 240

Query: 1151 VDGENPNLYLKSDDIVLCVLPLFHIFSLNSVLLCSLRTGSGVMLLQKFEIGAFLELIQKY 972
            VDGE PNLYLK DD+VLCVLPLFHIFSLNSVLLCSLR GS V+L+QKFEIG+ LELIQK+
Sbjct: 241  VDGEIPNLYLKQDDVVLCVLPLFHIFSLNSVLLCSLRAGSAVLLMQKFEIGSLLELIQKH 300

Query: 971  RVSXXXXXXXXXXXXAKNPMVESFDLSSIRIVLSGAAPLGKELEDALKSRVPQAIFGQGY 792
             VS            AKNPMV +FDLSSIR+VLSGAAPLGKELE+AL+SRVPQAI GQGY
Sbjct: 301  NVSVAAVVPPLVLALAKNPMVANFDLSSIRVVLSGAAPLGKELEEALRSRVPQAILGQGY 360

Query: 791  GMTEAGPVLSMCSAFAKQPFPTKSGSCGTVVRNAELKVVDPETGLSLRHNQPGEICIRGP 612
            GMTEAGPVLSMC AF+KQP PTKSGSCGTVVRNAELKV+DPETG SL  NQPGEICIRG 
Sbjct: 361  GMTEAGPVLSMCLAFSKQPLPTKSGSCGTVVRNAELKVIDPETGSSLGRNQPGEICIRGS 420

Query: 611  QIMKGYLNDPDASKSTIDVEGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELE 432
            QIMKGYLND +A+ + IDVEGWLHTGDIGYVDDD+E+FIVDRVKE+IKFKGFQVPPAELE
Sbjct: 421  QIMKGYLNDAEATANIIDVEGWLHTGDIGYVDDDDEIFIVDRVKEIIKFKGFQVPPAELE 480

Query: 431  ALLVSHPSIADAAVVPQKDVMAGEVPVAFVVPSKDFEITEEAVKDFIAKQVVFYKRLHKV 252
            ALLV+HPSIADAAVVPQKD +AGEVPVAFVV S D ++ EEAVKD+IAKQVVFYK+LHKV
Sbjct: 481  ALLVNHPSIADAAVVPQKDEVAGEVPVAFVVRSNDLDLNEEAVKDYIAKQVVFYKKLHKV 540

Query: 251  YFIHAIPKSPAGKILRKDLRAKLASSS 171
            +F+H+IPKS AGKILRKDLRAKLA+++
Sbjct: 541  FFVHSIPKSAAGKILRKDLRAKLATAT 567


>gb|ADU15550.1| 4CL [Gossypium hirsutum]
          Length = 574

 Score =  859 bits (2220), Expect = 0.0
 Identities = 431/568 (75%), Positives = 489/568 (86%), Gaps = 9/568 (1%)
 Frame = -3

Query: 1847 IVSPETTPHEVSISEIENPSSET---FIFRSKLPDIPISNHLPLHTYCFEKVSEFAEKPC 1677
            ++S    P E+   + +  S ET    IFRSKLPDIPISNHLPLHTYCFE +   ++KPC
Sbjct: 1    MISIADHPVELEPPKPDQLSPETPADHIFRSKLPDIPISNHLPLHTYCFENLPSLSDKPC 60

Query: 1676 LISGSSGKIYTFAETHLICQKTAVGLSKLGIKKGDVIMVLLQNCPEFVFSFMGASMIGAI 1497
            LISGSSGKIY+F+ETHLI QKTA GLSKLGI+KGD IM+LL NC EFVF+FMGASMIGA+
Sbjct: 61   LISGSSGKIYSFSETHLIAQKTAAGLSKLGIQKGDAIMILLHNCAEFVFAFMGASMIGAV 120

Query: 1496 TTTANPFYTSTEIFKQLSASNAKLIVTQSQYVDKLRDST------ENSPKIGEDFNVVTI 1335
            +TTANPFYTS EIFKQ  A+ AKLI+TQSQYVDKL+D+       EN PKIGEDF V+TI
Sbjct: 121  STTANPFYTSKEIFKQSKAARAKLIITQSQYVDKLKDTNNNNDGVENFPKIGEDFKVITI 180

Query: 1334 DDPPENCHHFSVISNADEKEIGSVSIHPDDPVALPFSSGTTGLPKGVILTHKSLISSVAQ 1155
            DDPPENC  FSV+S A+E EI  V+IHPDDPVALPFSSGTTGLPKGV+LTHKSLI+SVAQ
Sbjct: 181  DDPPENCLDFSVLSEANENEIPQVTIHPDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQ 240

Query: 1154 QVDGENPNLYLKSDDIVLCVLPLFHIFSLNSVLLCSLRTGSGVMLLQKFEIGAFLELIQK 975
            QVDGENPNLYL  +D+VLCVLPLFHI+SLNSVLL SLR G+ V+L+QKFEIG  LELIQ+
Sbjct: 241  QVDGENPNLYLTHEDVVLCVLPLFHIYSLNSVLLWSLRAGAAVLLMQKFEIGTLLELIQR 300

Query: 974  YRVSXXXXXXXXXXXXAKNPMVESFDLSSIRIVLSGAAPLGKELEDALKSRVPQAIFGQG 795
            ++VS            AKNP+V  FDLSSIR+VLSGAAPLGKELEDAL+ RVPQA+ GQG
Sbjct: 301  HKVSVAAVVPPLVVLLAKNPVVAQFDLSSIRVVLSGAAPLGKELEDALRGRVPQAVLGQG 360

Query: 794  YGMTEAGPVLSMCSAFAKQPFPTKSGSCGTVVRNAELKVVDPETGLSLRHNQPGEICIRG 615
            YGMTEAGPVLSMC  FAKQPFPTKSGSCGTVVRNAELKV+DPETG SL +NQPGEICIRG
Sbjct: 361  YGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICIRG 420

Query: 614  PQIMKGYLNDPDASKSTIDVEGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAEL 435
             QIMKGYLND  A+ +TIDVEGWLHTGDIGYVD+D+E+FIVDRVKE+IKFKGFQVPPAEL
Sbjct: 421  SQIMKGYLNDGVATAATIDVEGWLHTGDIGYVDEDDEIFIVDRVKEIIKFKGFQVPPAEL 480

Query: 434  EALLVSHPSIADAAVVPQKDVMAGEVPVAFVVPSKDFEITEEAVKDFIAKQVVFYKRLHK 255
            E+LL+SHPSIADAAVVPQKD +AGEVPVAFVV S  FE+TEEAVK+FIAKQVV+YKRLHK
Sbjct: 481  ESLLISHPSIADAAVVPQKDEVAGEVPVAFVVRSNGFELTEEAVKEFIAKQVVYYKRLHK 540

Query: 254  VYFIHAIPKSPAGKILRKDLRAKLASSS 171
            V+F+HAIPKSP+GKILRKDLRAKLA+++
Sbjct: 541  VHFVHAIPKSPSGKILRKDLRAKLAAAA 568


>gb|AAC24504.1| 4-coumarate:CoA ligase [Populus tremuloides]
          Length = 570

 Score =  854 bits (2206), Expect = 0.0
 Identities = 433/567 (76%), Positives = 492/567 (86%), Gaps = 5/567 (0%)
 Frame = -3

Query: 1856 MISIVSPETTPHEVSISEIEN-PSSET--FIFRSKLPDIPISNHLPLHTYCFEKVSEFAE 1686
            M+S+ + E    E+S  + +N PSS     IFRSKLPDI ISN LPLH YCFE +S+F++
Sbjct: 1    MMSVATVEPPKPELSPPQNQNAPSSHETDHIFRSKLPDITISNDLPLHAYCFENLSDFSD 60

Query: 1685 KPCLISGSSGKIYTFAETHLICQKTAVGLSKLGIKKGDVIMVLLQNCPEFVFSFMGASMI 1506
            +PCLISGS+GK Y+FAETHLI +K A GLS LGIKKGDVIM LLQNCPEFVFSF+GASMI
Sbjct: 61   RPCLISGSTGKTYSFAETHLISRKVAAGLSNLGIKKGDVIMTLLQNCPEFVFSFIGASMI 120

Query: 1505 GAITTTANPFYTSTEIFKQLSASNAKLIVTQSQYVDKLRDST--ENSPKIGEDFNVVTID 1332
            GA+ TTANPFYT +EIFKQ SAS AKLI+TQSQYV+KL DS   EN+ K GEDF V+TID
Sbjct: 121  GAVITTANPFYTQSEIFKQFSASRAKLIITQSQYVNKLGDSDCHENNQKPGEDFIVITID 180

Query: 1331 DPPENCHHFSVISNADEKEIGSVSIHPDDPVALPFSSGTTGLPKGVILTHKSLISSVAQQ 1152
            DPPENC HF+V+  A E E+ +VSI PDDPVALPFSSGTTGLPKGVILTHKSLI+SVAQQ
Sbjct: 181  DPPENCLHFNVLVEASESEMPTVSILPDDPVALPFSSGTTGLPKGVILTHKSLITSVAQQ 240

Query: 1151 VDGENPNLYLKSDDIVLCVLPLFHIFSLNSVLLCSLRTGSGVMLLQKFEIGAFLELIQKY 972
            VDGE PNLYLK DD+VLCVLPLFHIFSLNSVLLCSLR GS V+L+QKFEIG+ LELIQK+
Sbjct: 241  VDGEIPNLYLKQDDVVLCVLPLFHIFSLNSVLLCSLRAGSAVLLMQKFEIGSLLELIQKH 300

Query: 971  RVSXXXXXXXXXXXXAKNPMVESFDLSSIRIVLSGAAPLGKELEDALKSRVPQAIFGQGY 792
             VS            AKNP+  +FDLSSIR+VLSGAAPLGKELEDAL+SRVPQAI GQGY
Sbjct: 301  NVSVAAVVPPLVLALAKNPLEANFDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGY 360

Query: 791  GMTEAGPVLSMCSAFAKQPFPTKSGSCGTVVRNAELKVVDPETGLSLRHNQPGEICIRGP 612
            GMTEAGPVLSMC AF+KQPFPTKSGSCGTVVRNAELKV+DPETG SL +NQPGEICIRG 
Sbjct: 361  GMTEAGPVLSMCLAFSKQPFPTKSGSCGTVVRNAELKVIDPETGRSLGYNQPGEICIRGS 420

Query: 611  QIMKGYLNDPDASKSTIDVEGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELE 432
            QIMKGYLND +A+ +TIDVEGWLHTGDIGYVDDD+E+FIVDRVKE+IKFKGFQVPPAELE
Sbjct: 421  QIMKGYLNDAEATANTIDVEGWLHTGDIGYVDDDDEIFIVDRVKEIIKFKGFQVPPAELE 480

Query: 431  ALLVSHPSIADAAVVPQKDVMAGEVPVAFVVPSKDFEITEEAVKDFIAKQVVFYKRLHKV 252
            ALLV+HPSIADAAVVPQKD +AGEVPVAFVV S D +++EEAVK++IAKQVVFYK+LHKV
Sbjct: 481  ALLVNHPSIADAAVVPQKDEVAGEVPVAFVVRSDDLDLSEEAVKEYIAKQVVFYKKLHKV 540

Query: 251  YFIHAIPKSPAGKILRKDLRAKLASSS 171
            +F+H+IPKS +GKILRKDLRAKLA+++
Sbjct: 541  FFVHSIPKSASGKILRKDLRAKLATAT 567


>ref|XP_006443197.1| hypothetical protein CICLE_v10019532mg [Citrus clementina]
            gi|568850424|ref|XP_006478914.1| PREDICTED:
            4-coumarate--CoA ligase 2-like [Citrus sinensis]
            gi|557545459|gb|ESR56437.1| hypothetical protein
            CICLE_v10019532mg [Citrus clementina]
          Length = 560

 Score =  853 bits (2203), Expect = 0.0
 Identities = 432/566 (76%), Positives = 484/566 (85%), Gaps = 5/566 (0%)
 Frame = -3

Query: 1856 MISIVS--PETTPHEVSISEIENPSSE---TFIFRSKLPDIPISNHLPLHTYCFEKVSEF 1692
            MISI +  PE +  ++S      PS+E   T IF+SKLPDIPISNHLPLHTYCF+     
Sbjct: 1    MISIATKKPELSL-DISSPAPPAPSNEKIATHIFKSKLPDIPISNHLPLHTYCFQ--DRL 57

Query: 1691 AEKPCLISGSSGKIYTFAETHLICQKTAVGLSKLGIKKGDVIMVLLQNCPEFVFSFMGAS 1512
            ++ PCLI G +GK Y++AETHLIC+KTA GLS LGIKKGDVIM+LLQNC EFVFSFMGAS
Sbjct: 58   SDDPCLIVGLTGKTYSYAETHLICRKTAAGLSNLGIKKGDVIMILLQNCAEFVFSFMGAS 117

Query: 1511 MIGAITTTANPFYTSTEIFKQLSASNAKLIVTQSQYVDKLRDSTENSPKIGEDFNVVTID 1332
            MIGA+TTTANPFYTS EI KQ   S AKLI+T SQYVD+L       PK  +DF V+TID
Sbjct: 118  MIGAVTTTANPFYTSAEILKQFRTSGAKLIITMSQYVDRL-------PKTDKDFTVITID 170

Query: 1331 DPPENCHHFSVISNADEKEIGSVSIHPDDPVALPFSSGTTGLPKGVILTHKSLISSVAQQ 1152
             PPENC HF+V+S ADE +I  V+I PDDPVALPFSSGTTGLPKGV+LTHKSLI+SVAQQ
Sbjct: 171  APPENCLHFTVLSEADEDQIPEVAIEPDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQ 230

Query: 1151 VDGENPNLYLKSDDIVLCVLPLFHIFSLNSVLLCSLRTGSGVMLLQKFEIGAFLELIQKY 972
            VDGENPNLYL + D+VLCVLPLFHI+SLNSVLLCSLR G+GV+L+QKFEIGA LELIQ++
Sbjct: 231  VDGENPNLYLTNGDVVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMQKFEIGALLELIQRH 290

Query: 971  RVSXXXXXXXXXXXXAKNPMVESFDLSSIRIVLSGAAPLGKELEDALKSRVPQAIFGQGY 792
            RVS            AKNPMV  +DLSSIR+VLSGAAPLGKELEDAL+SRVPQAI GQGY
Sbjct: 291  RVSVAAVVPPLVLALAKNPMVADYDLSSIRVVLSGAAPLGKELEDALRSRVPQAILGQGY 350

Query: 791  GMTEAGPVLSMCSAFAKQPFPTKSGSCGTVVRNAELKVVDPETGLSLRHNQPGEICIRGP 612
            GMTEAGPVLSMC  FAKQPFPTKSGSCGTVVRNAELKV+DPE G SL HNQPGEICIRGP
Sbjct: 351  GMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPEIGASLPHNQPGEICIRGP 410

Query: 611  QIMKGYLNDPDASKSTIDVEGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELE 432
            QIMKGYLNDP+A+ +TIDVEGWLHTGDIGYVDDD+EVFIVDRVKE+IKFKGFQVPPAE+E
Sbjct: 411  QIMKGYLNDPEATAATIDVEGWLHTGDIGYVDDDDEVFIVDRVKEIIKFKGFQVPPAEIE 470

Query: 431  ALLVSHPSIADAAVVPQKDVMAGEVPVAFVVPSKDFEITEEAVKDFIAKQVVFYKRLHKV 252
            ALL+SHPSI DAAVVPQKD +AGEVPVAFVV S  FE+TEEA+K++IAKQVVFYKRLHK+
Sbjct: 471  ALLLSHPSIGDAAVVPQKDEVAGEVPVAFVVRSNGFELTEEAIKEYIAKQVVFYKRLHKI 530

Query: 251  YFIHAIPKSPAGKILRKDLRAKLASS 174
            YF+HAIPKSP+GKILRKDLRAKLASS
Sbjct: 531  YFVHAIPKSPSGKILRKDLRAKLASS 556


>gb|AAK58909.1| 4-coumarate:CoA ligase 4 [Populus trichocarpa x Populus deltoides]
          Length = 579

 Score =  851 bits (2198), Expect = 0.0
 Identities = 435/576 (75%), Positives = 491/576 (85%), Gaps = 14/576 (2%)
 Frame = -3

Query: 1856 MISIVSPETTPHEVSISEIEN-PSSET--FIFRSKLPDIPISNHLPLHTYCFEKVSEFAE 1686
            M+S+ + E    E+S  + +N PSS     IFRSKLPDI ISNHLPLH YCFE +S+F++
Sbjct: 1    MMSVATVEPPKPELSPPQNQNAPSSHETDHIFRSKLPDITISNHLPLHAYCFENLSDFSD 60

Query: 1685 KPCLISGSSGKIYTFAETHLICQKTAVGLSKLGIKKGDVIMVLLQNCPEFVFSFMGASMI 1506
            +PCLISGS+GK Y+FAETHLI +K A GLS LGIKKGDVIM LLQNCPEFVFSFMGASMI
Sbjct: 61   RPCLISGSTGKTYSFAETHLISRKVAAGLSNLGIKKGDVIMTLLQNCPEFVFSFMGASMI 120

Query: 1505 GAITTTANPFYTSTEIFKQLSASNAKLIVTQSQYVDKLRDST--ENSPKIGEDFNVVTID 1332
            GA+TTT NPFYT  EIFKQ SAS AKLI+TQSQ+V+KLRDS   EN+ K  EDF V+TID
Sbjct: 121  GAVTTTVNPFYTPGEIFKQFSASRAKLIITQSQHVNKLRDSDYHENNQKPEEDFIVITID 180

Query: 1331 DPPENCHHFSVISNADEKEIGSVSIHPDDPVALPFSSGTTGLPKGVILTHKSLISSVAQQ 1152
            DPPENC HF+V+  A+E E+ +VSIHPDDPVALPFSSGTTGLPKGVILTHKSLI+SVAQQ
Sbjct: 181  DPPENCLHFNVLVEANESEMPTVSIHPDDPVALPFSSGTTGLPKGVILTHKSLITSVAQQ 240

Query: 1151 VDGENPNLYLKSDDIVLCVLPLFHIFSLNSVLLCSLRTGSGVMLLQKFEIGAFLELIQKY 972
            VDGE PNLYLK DD+VLCVLPLFHIFSLNSVLLCSLR GS V+L+QKFEIG+ LELIQK+
Sbjct: 241  VDGEIPNLYLKQDDVVLCVLPLFHIFSLNSVLLCSLRAGSAVLLMQKFEIGSLLELIQKH 300

Query: 971  RVSXXXXXXXXXXXXAKNPMVESFDLSSIRIVLSGAAPLGKELEDALKSRVPQAIFGQGY 792
             VS            AKNPMV +FDLSSIR+VLSGAAPLGKELE+AL+SRVPQAI GQGY
Sbjct: 301  NVSVAAVVPPLVLALAKNPMVANFDLSSIRVVLSGAAPLGKELEEALRSRVPQAILGQGY 360

Query: 791  GMTEAGPVLSMCSAFAKQPFPTKSGSCGTVVRNAELKVVDPETGLSLRHNQPGEICIRGP 612
            GMTEAGPVLSMC AF+KQP PTKSGSCGTVVRNAELKV+DPETG SL  NQPGEICIRG 
Sbjct: 361  GMTEAGPVLSMCLAFSKQPLPTKSGSCGTVVRNAELKVIDPETGSSLGRNQPGEICIRGS 420

Query: 611  QIMKGYLNDPDASKSTIDVEGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELE 432
            QIMKGYLND +A+ + IDVEGWLHTGDIGYVDDD+E+FIVDRVKE+IKFKGFQVPPAELE
Sbjct: 421  QIMKGYLNDAEATANIIDVEGWLHTGDIGYVDDDDEIFIVDRVKEIIKFKGFQVPPAELE 480

Query: 431  ALLVSHPSIADAAVVP---------QKDVMAGEVPVAFVVPSKDFEITEEAVKDFIAKQV 279
            ALLV+HPSIADAAVVP         QKD +AGEVPVAFVV S D ++ EEAVKD+IAKQV
Sbjct: 481  ALLVNHPSIADAAVVPRDNLYGNNRQKDEVAGEVPVAFVVRSNDLDLNEEAVKDYIAKQV 540

Query: 278  VFYKRLHKVYFIHAIPKSPAGKILRKDLRAKLASSS 171
            VFYK+LHKV+F+H+IPKS +GKILRKDLRAKLA+++
Sbjct: 541  VFYKKLHKVFFVHSIPKSASGKILRKDLRAKLATAT 576


>ref|XP_004307864.1| PREDICTED: LOW QUALITY PROTEIN: 4-coumarate--CoA ligase 2-like
            [Fragaria vesca subsp. vesca]
          Length = 586

 Score =  850 bits (2196), Expect = 0.0
 Identities = 430/573 (75%), Positives = 490/573 (85%), Gaps = 10/573 (1%)
 Frame = -3

Query: 1859 TMISIVSPETTPHEVSIS-EIENPSSET-----FIFRSKLPDIP-ISNHLPLHTYCFEKV 1701
            T + + + + +P  +S S E ++P++ T      +F+SKLPD+P ISNHLPLHTYCFE V
Sbjct: 11   TSVVVETADISPTLISASPEKQHPATSTTLHDHHVFKSKLPDLPNISNHLPLHTYCFENV 70

Query: 1700 SEFAEKPCLISGSSGKIYTFAETHLICQKTAVGLSKLGIKKGDVIMVLLQNCPEFVFSFM 1521
            S+F+E+PCLI GS+GK Y+F+ETHLI QKT  GLSKLGI KGDVIM+LLQNC EFVF+FM
Sbjct: 71   SDFSERPCLIVGSTGKTYSFSETHLISQKTGAGLSKLGITKGDVIMILLQNCAEFVFAFM 130

Query: 1520 GASMIGAITTTANPFYTSTEIFKQLSASNAKLIVTQSQYVDKLRDSTENS---PKIGEDF 1350
            GASMIGA+TTTANPFYT++EIFKQ  ASNAKLI+TQS YVDKLR S  +S   PK+GE F
Sbjct: 131  GASMIGAVTTTANPFYTASEIFKQFKASNAKLIITQSIYVDKLRHSGPDSDQYPKLGEHF 190

Query: 1349 NVVTIDDPPENCHHFSVISNADEKEIGSVSIHPDDPVALPFSSGTTGLPKGVILTHKSLI 1170
             VVTIDDPPENC HFSV+S A+E E+  VSI P+DPVALPFSSGTTGLPKGVILTHKSLI
Sbjct: 191  QVVTIDDPPENCLHFSVLSGANESELPEVSIDPEDPVALPFSSGTTGLPKGVILTHKSLI 250

Query: 1169 SSVAQQVDGENPNLYLKSDDIVLCVLPLFHIFSLNSVLLCSLRTGSGVMLLQKFEIGAFL 990
            +SVAQQVDGENPNLYLK DD+VLCVLPLFHI+SLNSVLLCSLR G+ V+++ KFEIG  L
Sbjct: 251  TSVAQQVDGENPNLYLKEDDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLVMHKFEIGTLL 310

Query: 989  ELIQKYRVSXXXXXXXXXXXXAKNPMVESFDLSSIRIVLSGAAPLGKELEDALKSRVPQA 810
            ELIQ+YRV              +  MV  +DLSSIR+VLSGAAPLGKELE+AL+SRVPQA
Sbjct: 311  ELIQRYRVXCGSCGATAGDSAGEESMVADYDLSSIRVVLSGAAPLGKELEEALRSRVPQA 370

Query: 809  IFGQGYGMTEAGPVLSMCSAFAKQPFPTKSGSCGTVVRNAELKVVDPETGLSLRHNQPGE 630
            + GQGYGMTEAGPVLSMC AFAKQPFPTKSGSCGTVVRNAELKV++PETG SL +NQPGE
Sbjct: 371  VLGQGYGMTEAGPVLSMCLAFAKQPFPTKSGSCGTVVRNAELKVIEPETGRSLGYNQPGE 430

Query: 629  ICIRGPQIMKGYLNDPDASKSTIDVEGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQV 450
            ICIRG QIMKGYLND  A+ +T+DVEGWLHTGD+GYVDDD EVFIVDRVKELIKFKGFQV
Sbjct: 431  ICIRGSQIMKGYLNDDAATATTVDVEGWLHTGDVGYVDDDGEVFIVDRVKELIKFKGFQV 490

Query: 449  PPAELEALLVSHPSIADAAVVPQKDVMAGEVPVAFVVPSKDFEITEEAVKDFIAKQVVFY 270
            PPAELE+LLVSH SIADAAVVPQKD  AGEVPVAFVV S   E+TEEAVK+FIAKQVVFY
Sbjct: 491  PPAELESLLVSHQSIADAAVVPQKDDAAGEVPVAFVVRSNGNELTEEAVKEFIAKQVVFY 550

Query: 269  KRLHKVYFIHAIPKSPAGKILRKDLRAKLASSS 171
            KRLHKVYF+HAIPKSP+GKILRKDLRAKLA+++
Sbjct: 551  KRLHKVYFVHAIPKSPSGKILRKDLRAKLAAAT 583


>gb|AFG30056.1| 4-coumarate: CoA ligase [Malus hybrid cultivar]
          Length = 605

 Score =  848 bits (2192), Expect = 0.0
 Identities = 422/543 (77%), Positives = 471/543 (86%), Gaps = 8/543 (1%)
 Frame = -3

Query: 1775 IFRSKLPDIPISNHLPLHTYCFEKVSEFAEKPCLISGSSGKIYTFAETHLICQKTAVGLS 1596
            +FRSKLPDIPI NHLPLHTYCF+ + EF++KPCLI GS+GK Y+F+ETHLI QKT  GLS
Sbjct: 59   VFRSKLPDIPIPNHLPLHTYCFQNLPEFSDKPCLIVGSTGKSYSFSETHLISQKTGAGLS 118

Query: 1595 KLGIKKGDVIMVLLQNCPEFVFSFMGASMIGAITTTANPFYTSTEIFKQLSASNAKLIVT 1416
             LGI+KGDVIM+LLQNC EFVF+FMGAS+IGA+TTTANPFYTS EIFKQ+ A+NAKLI+T
Sbjct: 119  NLGIQKGDVIMILLQNCAEFVFAFMGASLIGAVTTTANPFYTSAEIFKQVKAANAKLIIT 178

Query: 1415 QSQYVDKLRD--------STENSPKIGEDFNVVTIDDPPENCHHFSVISNADEKEIGSVS 1260
            QSQYVDKLR+           N PK+GEDF VVTIDDPPENC HFSV S A+EKE+  V 
Sbjct: 179  QSQYVDKLREHPSSADGADQNNYPKLGEDFKVVTIDDPPENCLHFSVFSEANEKELPDVV 238

Query: 1259 IHPDDPVALPFSSGTTGLPKGVILTHKSLISSVAQQVDGENPNLYLKSDDIVLCVLPLFH 1080
            I  +DPVALPFSSGTTGLPKGVILTHKSL++SVAQQVDGENPNLYLK DD+VLCVLPLFH
Sbjct: 239  IDAEDPVALPFSSGTTGLPKGVILTHKSLVTSVAQQVDGENPNLYLKEDDVVLCVLPLFH 298

Query: 1079 IFSLNSVLLCSLRTGSGVMLLQKFEIGAFLELIQKYRVSXXXXXXXXXXXXAKNPMVESF 900
            IFSLNSVLLCSLR G+GV+L+ KFEIG  LELIQ+YRVS            AKNPMV  F
Sbjct: 299  IFSLNSVLLCSLRAGAGVLLMHKFEIGTLLELIQRYRVSVAAVVPPLVIALAKNPMVAEF 358

Query: 899  DLSSIRIVLSGAAPLGKELEDALKSRVPQAIFGQGYGMTEAGPVLSMCSAFAKQPFPTKS 720
            DLSSIR+VLSGAAPLGKELE+ALKSRVPQA+ GQGYGMTEAGPVLSMC AFAK+P PTKS
Sbjct: 359  DLSSIRVVLSGAAPLGKELEEALKSRVPQALLGQGYGMTEAGPVLSMCMAFAKEPMPTKS 418

Query: 719  GSCGTVVRNAELKVVDPETGLSLRHNQPGEICIRGPQIMKGYLNDPDASKSTIDVEGWLH 540
            GSCGTVVRNAELKV+D ETGLSL +NQ GEICIRG QIMKGYLND  A+ +T+D EGWLH
Sbjct: 419  GSCGTVVRNAELKVLDLETGLSLGYNQSGEICIRGSQIMKGYLNDVAATATTVDTEGWLH 478

Query: 539  TGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELEALLVSHPSIADAAVVPQKDVMAGE 360
            TGD+GYVDDD E+FIVDR KELIKFKGFQVPPAELE+LL+SHPSIADAAVVPQKD  AGE
Sbjct: 479  TGDVGYVDDDNEIFIVDRAKELIKFKGFQVPPAELESLLISHPSIADAAVVPQKDDAAGE 538

Query: 359  VPVAFVVPSKDFEITEEAVKDFIAKQVVFYKRLHKVYFIHAIPKSPAGKILRKDLRAKLA 180
            VPVAFVV S   E+TEEAVK+FIAKQVVFYKRLHKV+F+HAIPKS +GKILRKDLRAKLA
Sbjct: 539  VPVAFVVRSNGLELTEEAVKEFIAKQVVFYKRLHKVHFVHAIPKSASGKILRKDLRAKLA 598

Query: 179  SSS 171
            +++
Sbjct: 599  TAT 601


>gb|ADE96997.1| p-coumarate:CoA-ligase 3 [Sorbus aucuparia]
          Length = 605

 Score =  847 bits (2189), Expect = 0.0
 Identities = 420/543 (77%), Positives = 474/543 (87%), Gaps = 8/543 (1%)
 Frame = -3

Query: 1775 IFRSKLPDIPISNHLPLHTYCFEKVSEFAEKPCLISGSSGKIYTFAETHLICQKTAVGLS 1596
            +FRSKLPDI I NHLPLHTYCF+ + EF+++PCLI GS+GK Y+F+ETHLI QKT  GLS
Sbjct: 59   VFRSKLPDIAIPNHLPLHTYCFQNLPEFSDRPCLIVGSTGKSYSFSETHLISQKTGAGLS 118

Query: 1595 KLGIKKGDVIMVLLQNCPEFVFSFMGASMIGAITTTANPFYTSTEIFKQLSASNAKLIVT 1416
             LGI+KGDVIM+LLQNC EFVF+FMGAS+IGA+TTTANPFYT+ EIFKQ+ A+NAKLI+T
Sbjct: 119  NLGIQKGDVIMILLQNCAEFVFAFMGASLIGAVTTTANPFYTTAEIFKQVKAANAKLIIT 178

Query: 1415 QSQYVDKLRD--------STENSPKIGEDFNVVTIDDPPENCHHFSVISNADEKEIGSVS 1260
            QSQYV+KLR+           N PK+GEDF VVTIDDPPENC HFSV+S A+EKE+  V 
Sbjct: 179  QSQYVNKLREHPSSADGADQNNYPKLGEDFKVVTIDDPPENCLHFSVLSEANEKELPDVV 238

Query: 1259 IHPDDPVALPFSSGTTGLPKGVILTHKSLISSVAQQVDGENPNLYLKSDDIVLCVLPLFH 1080
            I  +DPVALPFSSGTTGLPKGVILTHK+L++SVAQQVDGENPNLYLK DD+VLCVLPLFH
Sbjct: 239  IDAEDPVALPFSSGTTGLPKGVILTHKNLVTSVAQQVDGENPNLYLKEDDVVLCVLPLFH 298

Query: 1079 IFSLNSVLLCSLRTGSGVMLLQKFEIGAFLELIQKYRVSXXXXXXXXXXXXAKNPMVESF 900
            IFS NSVLLCSLR G+GV+L+ KFEIG  LELIQ+YRVS            AKNPMV  F
Sbjct: 299  IFSFNSVLLCSLRAGAGVLLMHKFEIGTLLELIQRYRVSVAAVVPPLVIALAKNPMVAEF 358

Query: 899  DLSSIRIVLSGAAPLGKELEDALKSRVPQAIFGQGYGMTEAGPVLSMCSAFAKQPFPTKS 720
            DLSSIR+VLSGAAPLGKELE+ALKSRVPQA+ GQGYGMTEAGPVLSMC AFAK+P PTKS
Sbjct: 359  DLSSIRVVLSGAAPLGKELEEALKSRVPQAVLGQGYGMTEAGPVLSMCMAFAKEPMPTKS 418

Query: 719  GSCGTVVRNAELKVVDPETGLSLRHNQPGEICIRGPQIMKGYLNDPDASKSTIDVEGWLH 540
            GSCGTVVRNAELKV+DPETGLSL +NQPGEICIRG QIMKGYLND  A+ +TID EGWLH
Sbjct: 419  GSCGTVVRNAELKVLDPETGLSLGYNQPGEICIRGFQIMKGYLNDAAATATTIDTEGWLH 478

Query: 539  TGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELEALLVSHPSIADAAVVPQKDVMAGE 360
            TGD+GYVDDD+EVFIVDRVKELIKFKGFQVPPAELE+LL+SHPSIADAAVVPQ+D  AGE
Sbjct: 479  TGDVGYVDDDDEVFIVDRVKELIKFKGFQVPPAELESLLISHPSIADAAVVPQRDDAAGE 538

Query: 359  VPVAFVVPSKDFEITEEAVKDFIAKQVVFYKRLHKVYFIHAIPKSPAGKILRKDLRAKLA 180
            VPVAFVV S   E+TEEAVK+FIAKQVVFYKRLHKV+F+HAIPKS +GKILRKDLRAKLA
Sbjct: 539  VPVAFVVRSDGLELTEEAVKEFIAKQVVFYKRLHKVHFVHAIPKSASGKILRKDLRAKLA 598

Query: 179  SSS 171
            +++
Sbjct: 599  TAT 601


>gb|EYU45376.1| hypothetical protein MIMGU_mgv1a003734mg [Mimulus guttatus]
          Length = 567

 Score =  847 bits (2188), Expect = 0.0
 Identities = 423/567 (74%), Positives = 488/567 (86%), Gaps = 5/567 (0%)
 Frame = -3

Query: 1856 MISIVSPETTPHEVS---ISEIENPSSETFIFRSKLPDIPISNHLPLHTYCFEKVSEFAE 1686
            M+S+ S ET   E+S   I++ ++   ++ +F+SKLP IPISNHLPLHTYCFE  S + +
Sbjct: 1    MLSVASAETQNPELSSPIITQKQSSPEKSHVFKSKLPTIPISNHLPLHTYCFENFSHYPD 60

Query: 1685 KPCLISGS-SGKIYTFAETHLICQKTAVGLSKLGIKKGDVIMVLLQNCPEFVFSFMGASM 1509
            + CL+ G+ +GK YT+AETHLIC+K A GLS LGIKKGDV++VLLQNC EFVF+FMGASM
Sbjct: 61   RKCLLVGNGTGKSYTYAETHLICRKVAAGLSNLGIKKGDVVLVLLQNCAEFVFTFMGASM 120

Query: 1508 IGAITTTANPFYTSTEIFKQLSASNAKLIVTQSQYVDKLRDSTENSPKIGEDFNVVTIDD 1329
            IGA+TT ANPF T+ EIFKQ  ASN+++I+TQ+QYVDKLR+  ++SPK GEDF+V+T+DD
Sbjct: 121  IGAVTTAANPFCTANEIFKQFRASNSRMIITQAQYVDKLREGGDDSPKFGEDFSVITVDD 180

Query: 1328 PPENCHHFSVISNADEKEI-GSVSIHPDDPVALPFSSGTTGLPKGVILTHKSLISSVAQQ 1152
            PPE C HFSV+S A+E +   +V I  DD V+LPFSSGTTGLPKGVILTHKSLI+SVAQQ
Sbjct: 181  PPEGCLHFSVLSEANENDAPAAVEIDSDDAVSLPFSSGTTGLPKGVILTHKSLITSVAQQ 240

Query: 1151 VDGENPNLYLKSDDIVLCVLPLFHIFSLNSVLLCSLRTGSGVMLLQKFEIGAFLELIQKY 972
            VDGENPNLYLK DD+VLCVLPLFHI+SLNSV+LCSLR G+ V+L+QKFEIG+ LELIQ++
Sbjct: 241  VDGENPNLYLKPDDVVLCVLPLFHIYSLNSVMLCSLRAGAAVLLMQKFEIGSLLELIQRH 300

Query: 971  RVSXXXXXXXXXXXXAKNPMVESFDLSSIRIVLSGAAPLGKELEDALKSRVPQAIFGQGY 792
            RVS            AKNPMV+ FDLSSIR+VLSGAAPLGKELE AL SR+PQA+FGQGY
Sbjct: 301  RVSVAAVVPPLVLALAKNPMVDQFDLSSIRMVLSGAAPLGKELEAALLSRLPQAVFGQGY 360

Query: 791  GMTEAGPVLSMCSAFAKQPFPTKSGSCGTVVRNAELKVVDPETGLSLRHNQPGEICIRGP 612
            GMTEAGPVLSM  +FAKQP PTKSGSCG VVRNAELKVVDPETG S+  NQPGEICIRGP
Sbjct: 361  GMTEAGPVLSMSPSFAKQPLPTKSGSCGNVVRNAELKVVDPETGFSIPRNQPGEICIRGP 420

Query: 611  QIMKGYLNDPDASKSTIDVEGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELE 432
            QIMKGYLND +A+  TIDVEGWLHTGDIGYVDDD++VFIVDRVKELIKFKGFQVPPAELE
Sbjct: 421  QIMKGYLNDAEATARTIDVEGWLHTGDIGYVDDDDDVFIVDRVKELIKFKGFQVPPAELE 480

Query: 431  ALLVSHPSIADAAVVPQKDVMAGEVPVAFVVPSKDFEITEEAVKDFIAKQVVFYKRLHKV 252
            ALLVSHPSIADAAVVPQKD  AGEVPVAFVV S  FE+TEEAVKDFI+KQVVFYKRLHKV
Sbjct: 481  ALLVSHPSIADAAVVPQKDEAAGEVPVAFVVRSNGFELTEEAVKDFISKQVVFYKRLHKV 540

Query: 251  YFIHAIPKSPAGKILRKDLRAKLASSS 171
            YF+HAIPKSPAGKILRKDLRAKLA++S
Sbjct: 541  YFVHAIPKSPAGKILRKDLRAKLAAAS 567


>gb|AGW16013.1| 4-coumarate:coenzyme A ligase 1 [Pueraria montana var. lobata]
          Length = 567

 Score =  843 bits (2179), Expect = 0.0
 Identities = 419/562 (74%), Positives = 476/562 (84%), Gaps = 4/562 (0%)
 Frame = -3

Query: 1844 VSPETTPHEVSISEIENPS----SETFIFRSKLPDIPISNHLPLHTYCFEKVSEFAEKPC 1677
            V+P     EVS  + E         T +F+SKLPDIPISNH+PLH YCFEK+S+FA++PC
Sbjct: 4    VAPSVDAKEVSTPKTEQNQVCDPQTTHVFKSKLPDIPISNHIPLHAYCFEKLSDFADRPC 63

Query: 1676 LISGSSGKIYTFAETHLICQKTAVGLSKLGIKKGDVIMVLLQNCPEFVFSFMGASMIGAI 1497
            LI G++ K YT+AETHLI +K A GLS LGI+KGDV+M+LLQN  EFVFSF+ ASMIGA+
Sbjct: 64   LIVGAASKTYTYAETHLISRKIAAGLSNLGIRKGDVVMILLQNSAEFVFSFLAASMIGAV 123

Query: 1496 TTTANPFYTSTEIFKQLSASNAKLIVTQSQYVDKLRDSTENSPKIGEDFNVVTIDDPPEN 1317
             TTANPFYT+ EIFKQ + S AKLI+TQ+ YVDKLR    +   +GEDF VVT+DDPPEN
Sbjct: 124  GTTANPFYTAAEIFKQFTVSKAKLIITQAMYVDKLRH--HDGTNLGEDFKVVTVDDPPEN 181

Query: 1316 CHHFSVISNADEKEIGSVSIHPDDPVALPFSSGTTGLPKGVILTHKSLISSVAQQVDGEN 1137
            C HFSV+S A+E E+  V IHPDD VA+PFSSGTTGLPKGV+LTHKSL +SVAQQVDGEN
Sbjct: 182  CLHFSVLSEANESEVPEVEIHPDDAVAMPFSSGTTGLPKGVVLTHKSLTTSVAQQVDGEN 241

Query: 1136 PNLYLKSDDIVLCVLPLFHIFSLNSVLLCSLRTGSGVMLLQKFEIGAFLELIQKYRVSXX 957
            PNLYL ++D++LCVLPLFHIFSLNSVLLC+LR GSGV+L+QKFEIG  LELIQ++RVS  
Sbjct: 242  PNLYLTTEDVLLCVLPLFHIFSLNSVLLCALRAGSGVLLMQKFEIGTLLELIQRHRVSVA 301

Query: 956  XXXXXXXXXXAKNPMVESFDLSSIRIVLSGAAPLGKELEDALKSRVPQAIFGQGYGMTEA 777
                      AKNPMV  FDLSSIR+VLSGAAPLGKELE+AL++RVPQA+ GQGYGMTEA
Sbjct: 302  MVVPPLVLALAKNPMVADFDLSSIRLVLSGAAPLGKELEEALRNRVPQAVLGQGYGMTEA 361

Query: 776  GPVLSMCSAFAKQPFPTKSGSCGTVVRNAELKVVDPETGLSLRHNQPGEICIRGPQIMKG 597
            GPVLSMC  FAKQPFPTKSGSCGTVVRNAELKVVDPETG SL +NQPGEICIRG QIMKG
Sbjct: 362  GPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVVDPETGRSLGYNQPGEICIRGQQIMKG 421

Query: 596  YLNDPDASKSTIDVEGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELEALLVS 417
            YLND  A+  TID EGWLHTGD+GYVDDD+E+FIVDRVKELIKFKGFQVPPAELE LLVS
Sbjct: 422  YLNDEKATALTIDSEGWLHTGDVGYVDDDDEIFIVDRVKELIKFKGFQVPPAELEGLLVS 481

Query: 416  HPSIADAAVVPQKDVMAGEVPVAFVVPSKDFEITEEAVKDFIAKQVVFYKRLHKVYFIHA 237
            HPSIADAAVVPQKDV AGEVPVAFVV S  F++TEEAVK+FIAKQVVFYKRLHKVYF+HA
Sbjct: 482  HPSIADAAVVPQKDVAAGEVPVAFVVRSNGFDLTEEAVKEFIAKQVVFYKRLHKVYFVHA 541

Query: 236  IPKSPAGKILRKDLRAKLASSS 171
            IPKSP+GKILRKDLRAKL S++
Sbjct: 542  IPKSPSGKILRKDLRAKLESAA 563


>gb|ADZ54779.1| 4-coumarate-CoA ligase [Prunus avium]
          Length = 604

 Score =  843 bits (2177), Expect = 0.0
 Identities = 422/566 (74%), Positives = 480/566 (84%), Gaps = 8/566 (1%)
 Frame = -3

Query: 1850 SIVSPETTPHEVSISEIENPSSETFIFRSKLPDIPISNHLPLHTYCFEKVSEFAEKPCLI 1671
            +I SP TT      +     ++   +F+SKLPDIPI NHLPLHTYCF+K+SEF+++PCLI
Sbjct: 40   NIHSPPTT------AATTTTTTTNHVFKSKLPDIPIPNHLPLHTYCFQKLSEFSDRPCLI 93

Query: 1670 SGSSGKIYTFAETHLICQKTAVGLSKLGIKKGDVIMVLLQNCPEFVFSFMGASMIGAITT 1491
              S+GK Y+F++T+LI QKT  GLS LGIKKGDVIM+LLQNC EFVF+FMGASMIGAITT
Sbjct: 94   VSSTGKSYSFSDTYLISQKTGAGLSNLGIKKGDVIMILLQNCAEFVFAFMGASMIGAITT 153

Query: 1490 TANPFYTSTEIFKQLSASNAKLIVTQSQYVDKLRDST--------ENSPKIGEDFNVVTI 1335
            TANPFYT+TEIFKQ   SNAKLI+T SQ+VDKLR++          N  K+GE F VVT+
Sbjct: 154  TANPFYTATEIFKQFKNSNAKLIITHSQFVDKLREAPADGAAPDQNNYLKLGEHFKVVTV 213

Query: 1334 DDPPENCHHFSVISNADEKEIGSVSIHPDDPVALPFSSGTTGLPKGVILTHKSLISSVAQ 1155
            DDPPENC HFSVIS A+EKE+  V I P+DPVALPFSSGTTGLPKGVILTHKSLI+SVAQ
Sbjct: 214  DDPPENCLHFSVISEANEKELPEVVIDPEDPVALPFSSGTTGLPKGVILTHKSLITSVAQ 273

Query: 1154 QVDGENPNLYLKSDDIVLCVLPLFHIFSLNSVLLCSLRTGSGVMLLQKFEIGAFLELIQK 975
            QVDGENPNLYLK DD+VLCVLPLFHI+SLNSV+LCSLR G+ V+++ KFEIG  LELIQ+
Sbjct: 274  QVDGENPNLYLKEDDVVLCVLPLFHIYSLNSVMLCSLRAGAAVLVMHKFEIGTLLELIQR 333

Query: 974  YRVSXXXXXXXXXXXXAKNPMVESFDLSSIRIVLSGAAPLGKELEDALKSRVPQAIFGQG 795
            YRVS            AKNPMV  FDLSSIR+VLSGAAPLGKELE+AL+SRVP A+ GQG
Sbjct: 334  YRVSVAAVVPPLVIALAKNPMVAQFDLSSIRVVLSGAAPLGKELEEALRSRVPGAVLGQG 393

Query: 794  YGMTEAGPVLSMCSAFAKQPFPTKSGSCGTVVRNAELKVVDPETGLSLRHNQPGEICIRG 615
            YGMTEAGPVLSMC AFAK+P P+KSGSCGTV+RNAELKV+D ETG SL +NQPGEICIRG
Sbjct: 394  YGMTEAGPVLSMCLAFAKEPLPSKSGSCGTVIRNAELKVIDSETGCSLGYNQPGEICIRG 453

Query: 614  PQIMKGYLNDPDASKSTIDVEGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAEL 435
             QIMKGYLND +A+ +T+D EGWLHTGD+GYVDDDEEVFIVDRVKELIKFKGFQVPPAEL
Sbjct: 454  SQIMKGYLNDAEATATTVDKEGWLHTGDVGYVDDDEEVFIVDRVKELIKFKGFQVPPAEL 513

Query: 434  EALLVSHPSIADAAVVPQKDVMAGEVPVAFVVPSKDFEITEEAVKDFIAKQVVFYKRLHK 255
            E+LLVSHPSIADAAVVPQKD  AGEVPVAFVV S   ++TEEAVK+FIAKQVVFYKRLHK
Sbjct: 514  ESLLVSHPSIADAAVVPQKDEAAGEVPVAFVVRSNGLQLTEEAVKEFIAKQVVFYKRLHK 573

Query: 254  VYFIHAIPKSPAGKILRKDLRAKLAS 177
            VYF+HAIPKSP+GKILRKDLRAKLA+
Sbjct: 574  VYFVHAIPKSPSGKILRKDLRAKLAT 599