BLASTX nr result
ID: Akebia23_contig00056491
seq
BLASTX 2.2.25 [Feb-01-2011] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Akebia23_contig00056491 (325 letters) Database: ./nr 38,876,450 sequences; 13,856,398,315 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|ESZ92337.1| putative proline-specific permease put4 [Scleroti... 160 2e-37 ref|XP_001598775.1| hypothetical protein SS1G_00864 [Sclerotinia... 156 3e-36 gb|EPE29481.1| hypothetical protein GLAREA_00641 [Glarea lozoyen... 156 3e-36 gb|EHL03718.1| putative proline-specific permease put4 [Glarea l... 156 3e-36 emb|CCD44388.1| hypothetical protein BofuT4_P059680.1 [Botryotin... 154 1e-35 ref|XP_001552845.1| hypothetical protein BC1G_09027 [Botryotinia... 154 1e-35 ref|XP_003842189.1| hypothetical protein LEMA_P079490.1 [Leptosp... 154 1e-35 gb|ERT01438.1| hypothetical protein HMPREF1624_02685 [Sporothrix... 153 2e-35 gb|EME87689.1| hypothetical protein MYCFIDRAFT_70360 [Pseudocerc... 153 3e-35 gb|EAS30636.2| proline permease [Coccidioides immitis RS] 151 1e-34 gb|EOA90317.1| hypothetical protein SETTUDRAFT_167216 [Setosphae... 150 2e-34 gb|ETI19577.1| hypothetical protein G647_09411 [Cladophialophora... 150 2e-34 ref|XP_003173375.1| arginine permease [Arthroderma gypseum CBS 1... 150 2e-34 gb|EER36512.1| proline-specific permease [Ajellomyces capsulatus... 149 3e-34 gb|EEH06253.1| proline-specific permease [Ajellomyces capsulatus... 149 3e-34 ref|XP_001801023.1| hypothetical protein SNOG_10763 [Phaeosphaer... 149 3e-34 ref|XP_001536658.1| conserved hypothetical protein [Ajellomyces ... 149 3e-34 ref|XP_001940254.1| amino-acid permease inda1 [Pyrenophora triti... 149 5e-34 gb|EKG20418.1| Amino acid/polyamine transporter I [Macrophomina ... 148 7e-34 ref|XP_003295577.1| hypothetical protein PTT_01702 [Pyrenophora ... 148 7e-34 >gb|ESZ92337.1| putative proline-specific permease put4 [Sclerotinia borealis F-4157] Length = 587 Score = 160 bits (405), Expect = 2e-37 Identities = 81/108 (75%), Positives = 94/108 (87%), Gaps = 1/108 (0%) Frame = +2 Query: 2 SASSSANAFLFTGSRYLFALAQNGQAPKIFLKCSKSGVPYYAVLVTASISLLTYMSVSS- 178 SASSSANAFL+TGSRYLFALAQN QAP+ L+CSK+GVPY+AVL+TASIS LTY+SVSS Sbjct: 376 SASSSANAFLYTGSRYLFALAQNKQAPRFLLRCSKAGVPYWAVLITASISGLTYLSVSSG 435 Query: 179 GASTVFNWFQNLCSIANLITWINILIAYIQFRKALIAQGVDRNSLVMK 322 GAST F WFQNL +IA+L TW +I +AYIQF K+L AQGVDRN+LV K Sbjct: 436 GASTAFGWFQNLTTIASLFTWCSICVAYIQFHKSLAAQGVDRNTLVFK 483 >ref|XP_001598775.1| hypothetical protein SS1G_00864 [Sclerotinia sclerotiorum 1980] gi|154691723|gb|EDN91461.1| hypothetical protein SS1G_00864 [Sclerotinia sclerotiorum 1980 UF-70] Length = 573 Score = 156 bits (395), Expect = 3e-36 Identities = 80/108 (74%), Positives = 92/108 (85%), Gaps = 1/108 (0%) Frame = +2 Query: 2 SASSSANAFLFTGSRYLFALAQNGQAPKIFLKCSKSGVPYYAVLVTASISLLTYMSV-SS 178 SA+SSANAFL+TGSRYLFALAQN QAPK L+CSK+GVPY+AVL TASISL+TY+SV S Sbjct: 362 SATSSANAFLYTGSRYLFALAQNKQAPKFLLRCSKAGVPYWAVLCTASISLITYLSVGSG 421 Query: 179 GASTVFNWFQNLCSIANLITWINILIAYIQFRKALIAQGVDRNSLVMK 322 GAS F WFQNL +IA+L TW +I +AYIQF KAL AQGVDRN+LV K Sbjct: 422 GASAAFAWFQNLTTIASLFTWCSICVAYIQFYKALEAQGVDRNTLVFK 469 >gb|EPE29481.1| hypothetical protein GLAREA_00641 [Glarea lozoyensis ATCC 20868] Length = 563 Score = 156 bits (394), Expect = 3e-36 Identities = 80/108 (74%), Positives = 91/108 (84%), Gaps = 1/108 (0%) Frame = +2 Query: 2 SASSSANAFLFTGSRYLFALAQNGQAPKIFLKCSKSGVPYYAVLVTASISLLTYMSVSSG 181 SASSSANAFL+TGSRYLFALAQN QAP+IFL CSK+GVPYYAV VTASIS LTY+SV G Sbjct: 352 SASSSANAFLYTGSRYLFALAQNRQAPRIFLTCSKAGVPYYAVAVTASISGLTYLSVGKG 411 Query: 182 AST-VFNWFQNLCSIANLITWINILIAYIQFRKALIAQGVDRNSLVMK 322 S F WFQN+ +IA+L TW++I IAYIQF KAL AQG+DRN+LV K Sbjct: 412 GSAKAFAWFQNIVTIASLWTWVSICIAYIQFHKALKAQGIDRNTLVFK 459 >gb|EHL03718.1| putative proline-specific permease put4 [Glarea lozoyensis 74030] Length = 638 Score = 156 bits (394), Expect = 3e-36 Identities = 80/108 (74%), Positives = 91/108 (84%), Gaps = 1/108 (0%) Frame = +2 Query: 2 SASSSANAFLFTGSRYLFALAQNGQAPKIFLKCSKSGVPYYAVLVTASISLLTYMSVSSG 181 SASSSANAFL+TGSRYLFALAQN QAP+IFL CSK+GVPYYAV VTASIS LTY+SV G Sbjct: 427 SASSSANAFLYTGSRYLFALAQNRQAPRIFLTCSKAGVPYYAVAVTASISGLTYLSVGKG 486 Query: 182 AST-VFNWFQNLCSIANLITWINILIAYIQFRKALIAQGVDRNSLVMK 322 S F WFQN+ +IA+L TW++I IAYIQF KAL AQG+DRN+LV K Sbjct: 487 GSAKAFAWFQNIVTIASLWTWVSICIAYIQFHKALKAQGIDRNTLVFK 534 >emb|CCD44388.1| hypothetical protein BofuT4_P059680.1 [Botryotinia fuckeliana T4] Length = 217 Score = 154 bits (390), Expect = 1e-35 Identities = 77/108 (71%), Positives = 92/108 (85%), Gaps = 1/108 (0%) Frame = +2 Query: 2 SASSSANAFLFTGSRYLFALAQNGQAPKIFLKCSKSGVPYYAVLVTASISLLTYMSV-SS 178 SA+SSANAFL+TGSRYLFALAQN QAPK L+CSK+GVPY+AVL TASISL+T++SV S Sbjct: 6 SATSSANAFLYTGSRYLFALAQNKQAPKFLLRCSKTGVPYWAVLCTASISLITFLSVGSG 65 Query: 179 GASTVFNWFQNLCSIANLITWINILIAYIQFRKALIAQGVDRNSLVMK 322 GA+T F WFQNL +IA+L TW +I +AYI+F AL AQGVDRN+LV K Sbjct: 66 GAATAFTWFQNLTTIASLFTWCSICVAYIKFHSALAAQGVDRNTLVFK 113 >ref|XP_001552845.1| hypothetical protein BC1G_09027 [Botryotinia fuckeliana B05.10] gi|472238084|gb|EMR82960.1| putative amino acid transporter protein [Botryotinia fuckeliana BcDW1] Length = 575 Score = 154 bits (390), Expect = 1e-35 Identities = 77/108 (71%), Positives = 92/108 (85%), Gaps = 1/108 (0%) Frame = +2 Query: 2 SASSSANAFLFTGSRYLFALAQNGQAPKIFLKCSKSGVPYYAVLVTASISLLTYMSV-SS 178 SA+SSANAFL+TGSRYLFALAQN QAPK L+CSK+GVPY+AVL TASISL+T++SV S Sbjct: 364 SATSSANAFLYTGSRYLFALAQNKQAPKFLLRCSKTGVPYWAVLCTASISLITFLSVGSG 423 Query: 179 GASTVFNWFQNLCSIANLITWINILIAYIQFRKALIAQGVDRNSLVMK 322 GA+T F WFQNL +IA+L TW +I +AYI+F AL AQGVDRN+LV K Sbjct: 424 GAATAFTWFQNLTTIASLFTWCSICVAYIKFHSALAAQGVDRNTLVFK 471 >ref|XP_003842189.1| hypothetical protein LEMA_P079490.1 [Leptosphaeria maculans JN3] gi|312218765|emb|CBX98710.1| hypothetical protein LEMA_P079490.1 [Leptosphaeria maculans JN3] Length = 722 Score = 154 bits (389), Expect = 1e-35 Identities = 74/107 (69%), Positives = 88/107 (82%) Frame = +2 Query: 2 SASSSANAFLFTGSRYLFALAQNGQAPKIFLKCSKSGVPYYAVLVTASISLLTYMSVSSG 181 SA+SS NAFL+TGSRYLF +AQNGQAPK LKC++ GVP Y V++TASI L+TYMSVS+G Sbjct: 514 SATSSGNAFLYTGSRYLFGVAQNGQAPKFLLKCTRGGVPIYCVVITASIGLITYMSVSTG 573 Query: 182 ASTVFNWFQNLCSIANLITWINILIAYIQFRKALIAQGVDRNSLVMK 322 A+ VF WFQNL +IA L TW +I YI FRKAL+AQGVDRN+LV K Sbjct: 574 ANKVFLWFQNLTTIAQLFTWCSICYTYIGFRKALLAQGVDRNTLVFK 620 >gb|ERT01438.1| hypothetical protein HMPREF1624_02685 [Sporothrix schenckii ATCC 58251] Length = 561 Score = 153 bits (387), Expect = 2e-35 Identities = 76/108 (70%), Positives = 90/108 (83%), Gaps = 1/108 (0%) Frame = +2 Query: 2 SASSSANAFLFTGSRYLFALAQNGQAPKIFLKCSKSGVPYYAVLVTASISLLTYMSV-SS 178 SASSSANAFL+TGSRYL+ALAQN QAP IFL CS+ GVPYYAV TA++ LLTY+S S Sbjct: 353 SASSSANAFLYTGSRYLYALAQNRQAPAIFLTCSRRGVPYYAVAATAAVGLLTYLSAGSG 412 Query: 179 GASTVFNWFQNLCSIANLITWINILIAYIQFRKALIAQGVDRNSLVMK 322 GA+T FNWFQNL +IA+L TW +I + YI+F KAL AQG+DRN+LVMK Sbjct: 413 GAATAFNWFQNLTTIASLFTWGSICVCYIRFHKALKAQGIDRNTLVMK 460 >gb|EME87689.1| hypothetical protein MYCFIDRAFT_70360 [Pseudocercospora fijiensis CIRAD86] Length = 562 Score = 153 bits (386), Expect = 3e-35 Identities = 73/107 (68%), Positives = 90/107 (84%) Frame = +2 Query: 2 SASSSANAFLFTGSRYLFALAQNGQAPKIFLKCSKSGVPYYAVLVTASISLLTYMSVSSG 181 SASSS NAFL+TGSRYLFALAQN QAP+ L C+K GVP + VL+TASI LLTYM+VS+G Sbjct: 352 SASSSGNAFLYTGSRYLFALAQNKQAPRFLLYCTKKGVPIWCVLITASIGLLTYMTVSAG 411 Query: 182 ASTVFNWFQNLCSIANLITWINILIAYIQFRKALIAQGVDRNSLVMK 322 AS+VF+WFQ L +I +L TW +IL+AYI+FR+ALIAQGV RN L+ + Sbjct: 412 ASSVFDWFQTLTTITSLFTWFSILVAYIKFRRALIAQGVSRNDLIFR 458 >gb|EAS30636.2| proline permease [Coccidioides immitis RS] Length = 554 Score = 151 bits (381), Expect = 1e-34 Identities = 72/107 (67%), Positives = 90/107 (84%) Frame = +2 Query: 2 SASSSANAFLFTGSRYLFALAQNGQAPKIFLKCSKSGVPYYAVLVTASISLLTYMSVSSG 181 SASSSANAFL+TGSRYL+ALAQNG AP+ LKCSKSGVP Y VL+TA ISLLTY+S ++G Sbjct: 351 SASSSANAFLYTGSRYLYALAQNGHAPRFLLKCSKSGVPIYCVLITALISLLTYLSTTAG 410 Query: 182 ASTVFNWFQNLCSIANLITWINILIAYIQFRKALIAQGVDRNSLVMK 322 ++ VF WFQNL +IA+L TW +I +AYI+F AL AQGVDR++L+ + Sbjct: 411 SNVVFVWFQNLTTIASLFTWCSICVAYIKFHNALKAQGVDRDTLIFR 457 >gb|EOA90317.1| hypothetical protein SETTUDRAFT_167216 [Setosphaeria turcica Et28A] Length = 563 Score = 150 bits (379), Expect = 2e-34 Identities = 72/107 (67%), Positives = 88/107 (82%) Frame = +2 Query: 2 SASSSANAFLFTGSRYLFALAQNGQAPKIFLKCSKSGVPYYAVLVTASISLLTYMSVSSG 181 SA+SSANAFL+TGSRYLF++AQN QAP+ LKC+KSGVP Y V +TASISLLTYMSVS+G Sbjct: 355 SATSSANAFLYTGSRYLFSIAQNKQAPQFLLKCNKSGVPVYCVAITASISLLTYMSVSAG 414 Query: 182 ASTVFNWFQNLCSIANLITWINILIAYIQFRKALIAQGVDRNSLVMK 322 A+ VF WFQNL +IA+L TW I Y+ FRKA IAQ VDR+++V + Sbjct: 415 ANKVFTWFQNLTTIASLFTWCTICYTYVHFRKACIAQNVDRSTMVFR 461 >gb|ETI19577.1| hypothetical protein G647_09411 [Cladophialophora carrionii CBS 160.54] Length = 566 Score = 150 bits (378), Expect = 2e-34 Identities = 73/107 (68%), Positives = 89/107 (83%) Frame = +2 Query: 2 SASSSANAFLFTGSRYLFALAQNGQAPKIFLKCSKSGVPYYAVLVTASISLLTYMSVSSG 181 SASSSANAFL+TGSRYL+A+AQN QAP+ LKCSK+GVP + VL+T SISLLTYMS SSG Sbjct: 362 SASSSANAFLYTGSRYLYAIAQNRQAPRFLLKCSKTGVPIWCVLITWSISLLTYMSCSSG 421 Query: 182 ASTVFNWFQNLCSIANLITWINILIAYIQFRKALIAQGVDRNSLVMK 322 ++TVF WFQNL +I+ L TW++I IAYI+F A AQG+DRN+L K Sbjct: 422 SNTVFTWFQNLTTISTLFTWVSITIAYIRFYHACKAQGIDRNTLPFK 468 >ref|XP_003173375.1| arginine permease [Arthroderma gypseum CBS 118893] gi|311341342|gb|EFR00545.1| arginine permease [Arthroderma gypseum CBS 118893] Length = 558 Score = 150 bits (378), Expect = 2e-34 Identities = 69/107 (64%), Positives = 93/107 (86%) Frame = +2 Query: 2 SASSSANAFLFTGSRYLFALAQNGQAPKIFLKCSKSGVPYYAVLVTASISLLTYMSVSSG 181 SA+SS NAFL+TGSRYLF+LAQN QAP+ L+C+K+GVP YAVLVTAS+SLLT+M+ S+G Sbjct: 355 SATSSGNAFLYTGSRYLFSLAQNRQAPRFLLQCTKAGVPIYAVLVTASVSLLTFMAASTG 414 Query: 182 ASTVFNWFQNLCSIANLITWINILIAYIQFRKALIAQGVDRNSLVMK 322 ++ VF WFQNL ++A+L W+++L+AYI+F AL AQGVDRN+L++K Sbjct: 415 SAQVFIWFQNLTTVASLFNWVSVLVAYIRFHAALKAQGVDRNTLLLK 461 >gb|EER36512.1| proline-specific permease [Ajellomyces capsulatus H143] gi|325095696|gb|EGC49006.1| proline-specific permease [Ajellomyces capsulatus H88] Length = 557 Score = 149 bits (377), Expect = 3e-34 Identities = 71/107 (66%), Positives = 89/107 (83%) Frame = +2 Query: 2 SASSSANAFLFTGSRYLFALAQNGQAPKIFLKCSKSGVPYYAVLVTASISLLTYMSVSSG 181 SASSS NAFL+TGSRYL+ALAQNGQAP+ LKC+K GVP Y VL+T S+ LLT+MS S+G Sbjct: 353 SASSSGNAFLYTGSRYLYALAQNGQAPRFLLKCTKLGVPIYCVLITGSVGLLTFMSTSAG 412 Query: 182 ASTVFNWFQNLCSIANLITWINILIAYIQFRKALIAQGVDRNSLVMK 322 A+TVF WFQNL +IA+L TW++I IA I+F A+ AQGVDRN++V + Sbjct: 413 ANTVFIWFQNLTTIASLFTWVSICIACIKFHHAINAQGVDRNTMVFR 459 >gb|EEH06253.1| proline-specific permease [Ajellomyces capsulatus G186AR] Length = 557 Score = 149 bits (377), Expect = 3e-34 Identities = 71/107 (66%), Positives = 89/107 (83%) Frame = +2 Query: 2 SASSSANAFLFTGSRYLFALAQNGQAPKIFLKCSKSGVPYYAVLVTASISLLTYMSVSSG 181 SASSS NAFL+TGSRYL+ALAQNGQAP+ LKC+K GVP Y VL+T S+ LLT+MS S+G Sbjct: 353 SASSSGNAFLYTGSRYLYALAQNGQAPRFLLKCTKLGVPIYCVLITGSVGLLTFMSTSAG 412 Query: 182 ASTVFNWFQNLCSIANLITWINILIAYIQFRKALIAQGVDRNSLVMK 322 A+TVF WFQNL +IA+L TW++I IA I+F A+ AQGVDRN++V + Sbjct: 413 ANTVFIWFQNLTTIASLFTWVSICIACIKFHHAINAQGVDRNTMVFR 459 >ref|XP_001801023.1| hypothetical protein SNOG_10763 [Phaeosphaeria nodorum SN15] gi|160702909|gb|EAT82157.2| hypothetical protein SNOG_10763 [Phaeosphaeria nodorum SN15] Length = 575 Score = 149 bits (377), Expect = 3e-34 Identities = 71/105 (67%), Positives = 87/105 (82%) Frame = +2 Query: 2 SASSSANAFLFTGSRYLFALAQNGQAPKIFLKCSKSGVPYYAVLVTASISLLTYMSVSSG 181 SA+S+ NAFL+TGSRYLF +AQNG APK LKCSK+GVPYY V +T S+SLLTY+SVS+G Sbjct: 367 SATSAGNAFLYTGSRYLFGIAQNGHAPKFLLKCSKAGVPYYCVGITCSVSLLTYLSVSTG 426 Query: 182 ASTVFNWFQNLCSIANLITWINILIAYIQFRKALIAQGVDRNSLV 316 A+ VF WFQNL +IA L TW +I YI+FRKAL AQGVDR++L+ Sbjct: 427 ANKVFLWFQNLTTIAQLFTWSSICFTYIRFRKALAAQGVDRSTLL 471 >ref|XP_001536658.1| conserved hypothetical protein [Ajellomyces capsulatus NAm1] gi|150409328|gb|EDN04778.1| conserved hypothetical protein [Ajellomyces capsulatus NAm1] Length = 557 Score = 149 bits (377), Expect = 3e-34 Identities = 71/107 (66%), Positives = 89/107 (83%) Frame = +2 Query: 2 SASSSANAFLFTGSRYLFALAQNGQAPKIFLKCSKSGVPYYAVLVTASISLLTYMSVSSG 181 SASSS NAFL+TGSRYL+ALAQNGQAP+ LKC+K GVP Y VL+T S+ LLT+MS S+G Sbjct: 353 SASSSGNAFLYTGSRYLYALAQNGQAPRFLLKCTKLGVPIYCVLITGSVGLLTFMSTSAG 412 Query: 182 ASTVFNWFQNLCSIANLITWINILIAYIQFRKALIAQGVDRNSLVMK 322 A+TVF WFQNL +IA+L TW++I IA I+F A+ AQGVDRN++V + Sbjct: 413 ANTVFIWFQNLTTIASLFTWVSICIACIKFHHAINAQGVDRNTMVFR 459 >ref|XP_001940254.1| amino-acid permease inda1 [Pyrenophora tritici-repentis Pt-1C-BFP] gi|187976347|gb|EDU42973.1| amino-acid permease inda1 [Pyrenophora tritici-repentis Pt-1C-BFP] Length = 563 Score = 149 bits (375), Expect = 5e-34 Identities = 74/107 (69%), Positives = 85/107 (79%) Frame = +2 Query: 2 SASSSANAFLFTGSRYLFALAQNGQAPKIFLKCSKSGVPYYAVLVTASISLLTYMSVSSG 181 SA+SS NAFL+TGSRYLF +AQN QAP+ LKC+K GVP Y V VTASISLLTYMSVS+G Sbjct: 355 SATSSGNAFLYTGSRYLFGIAQNKQAPQFLLKCNKQGVPIYCVAVTASISLLTYMSVSTG 414 Query: 182 ASTVFNWFQNLCSIANLITWINILIAYIQFRKALIAQGVDRNSLVMK 322 AS VF WFQNL +IA+L TW +I Y+ FRKA IAQ VDRN LV + Sbjct: 415 ASKVFAWFQNLVTIASLFTWCSICYTYVCFRKAQIAQNVDRNELVFR 461 >gb|EKG20418.1| Amino acid/polyamine transporter I [Macrophomina phaseolina MS6] Length = 559 Score = 148 bits (374), Expect = 7e-34 Identities = 72/107 (67%), Positives = 86/107 (80%) Frame = +2 Query: 2 SASSSANAFLFTGSRYLFALAQNGQAPKIFLKCSKSGVPYYAVLVTASISLLTYMSVSSG 181 SASSSANAFLFTGSRYLF +AQN QAPK LKCS++GVPYY V VTA++SLLTYMSVSSG Sbjct: 354 SASSSANAFLFTGSRYLFGIAQNNQAPKFLLKCSRTGVPYYCVGVTAAVSLLTYMSVSSG 413 Query: 182 ASTVFNWFQNLCSIANLITWINILIAYIQFRKALIAQGVDRNSLVMK 322 A VF W N+ +IA L TW ++L+AYI+FR+A AQGV + L M+ Sbjct: 414 AGVVFEWMMNIVTIAQLFTWCSVLVAYIRFRQACHAQGVTDDQLPMR 460 >ref|XP_003295577.1| hypothetical protein PTT_01702 [Pyrenophora teres f. teres 0-1] gi|311333030|gb|EFQ96327.1| hypothetical protein PTT_01702 [Pyrenophora teres f. teres 0-1] Length = 563 Score = 148 bits (374), Expect = 7e-34 Identities = 73/107 (68%), Positives = 85/107 (79%) Frame = +2 Query: 2 SASSSANAFLFTGSRYLFALAQNGQAPKIFLKCSKSGVPYYAVLVTASISLLTYMSVSSG 181 SA+SS NAFL+TGSRYLF +AQN QAP+ LKC+K GVP Y V VTAS+SLLTYMSVS+G Sbjct: 355 SATSSGNAFLYTGSRYLFGIAQNRQAPQFLLKCNKQGVPIYCVAVTASVSLLTYMSVSTG 414 Query: 182 ASTVFNWFQNLCSIANLITWINILIAYIQFRKALIAQGVDRNSLVMK 322 AS VF WFQNL +IA+L TW +I Y+ FRKA IAQ VDRN LV + Sbjct: 415 ASKVFAWFQNLVTIASLFTWCSICYTYVCFRKAQIAQNVDRNELVFR 461