BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Akebia23_contig00026995
         (907 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI16383.3| unnamed protein product [Vitis vinifera]              434   e-119
ref|XP_002282827.1| PREDICTED: tRNA-specific 2-thiouridylase mnm...   434   e-119
ref|XP_006470603.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   414   e-113
gb|EXB93298.1| tRNA-specific 2-thiouridylase mnmA [Morus notabilis]   411   e-112
ref|XP_007209892.1| hypothetical protein PRUPE_ppa004048mg [Prun...   405   e-111
ref|XP_004142116.1| PREDICTED: tRNA-specific 2-thiouridylase Mnm...   405   e-111
ref|XP_004507093.1| PREDICTED: tRNA-specific 2-thiouridylase Mnm...   394   e-107
ref|XP_002298404.2| hypothetical protein POPTR_0001s26620g [Popu...   392   e-106
ref|XP_006850208.1| hypothetical protein AMTR_s00022p00251260 [A...   388   e-105
ref|XP_006590965.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   387   e-105
ref|XP_003539065.2| PREDICTED: mitochondrial tRNA-specific 2-thi...   387   e-105
ref|XP_007132017.1| hypothetical protein PHAVU_011G059600g [Phas...   386   e-105
ref|XP_007132016.1| hypothetical protein PHAVU_011G059600g [Phas...   386   e-105
ref|XP_002513510.1| tRNA (5-methylaminomethyl-2-thiouridylate)-m...   386   e-105
ref|XP_004301461.1| PREDICTED: tRNA-specific 2-thiouridylase Mnm...   367   3e-99
ref|XP_006393065.1| hypothetical protein EUTSA_v10011432mg [Eutr...   364   2e-98
ref|XP_006358437.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   362   9e-98
ref|XP_002894309.1| tRNA (5-methylaminomethyl-2-thiouridylate)-m...   357   3e-96
ref|XP_006280360.1| hypothetical protein CARUB_v10026287mg [Caps...   357   5e-96
ref|XP_004247484.1| PREDICTED: tRNA-specific 2-thiouridylase Mnm...   356   6e-96

>emb|CBI16383.3| unnamed protein product [Vitis vinifera]
          Length = 544

 Score =  434 bits (1115), Expect = e-119
 Identities = 205/283 (72%), Positives = 241/283 (85%), Gaps = 1/283 (0%)
 Frame = +2

Query: 53   KDEVRRLATRMDLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGILLEAETGDFLGKHH 232
            KDEVR LAT++DLPN+DRKDSQGICFLGKIKFSEFVARHIGE EGI+LEAETGD LGKH 
Sbjct: 264  KDEVRSLATKLDLPNRDRKDSQGICFLGKIKFSEFVARHIGEKEGIILEAETGDLLGKHR 323

Query: 233  GFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKQRRRFSVGSMKWLSGSP 412
            GFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFS DK+RR F VGS+KW+S  P
Sbjct: 324  GFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSLDKRRRLFRVGSLKWISREP 383

Query: 413  PDQVDQLQCKVRHGPSFYDCSLTMEPGEDNQGDSFIVHISEDDQGLAAGQFTAFYQGKTC 592
            P Q++QLQCKVRHGP FY+CS TMEPGED   D  +V +SEDDQGLAAGQFTAFYQG++C
Sbjct: 384  PGQINQLQCKVRHGPGFYNCSFTMEPGEDGHEDVAVVQLSEDDQGLAAGQFTAFYQGRSC 443

Query: 593  LGSGVIVESKDDEGFPVCARAMEIARMEDKSKLGKPIKIKVKPDSSPKNSELKETVELHR 772
            +GSGVI+ES DD+GFPVCA+A+EIARMEDKSKLGKP+KIKV+P++ PK S+  + ++L R
Sbjct: 444  IGSGVILESWDDQGFPVCAKALEIARMEDKSKLGKPVKIKVRPETPPKGSDQIDGIKLDR 503

Query: 773  ESVSPQ-LINNDVIDSQTCASRGLFSSIPMNWLQKLREKWMQI 898
            E  + Q  + +++  S    +R  F   PMNWLQK+REKW+Q+
Sbjct: 504  ELANSQAAVTDEISRSSEGEARSRF---PMNWLQKIREKWLQM 543


>ref|XP_002282827.1| PREDICTED: tRNA-specific 2-thiouridylase mnmA-like [Vitis vinifera]
          Length = 540

 Score =  434 bits (1115), Expect = e-119
 Identities = 205/283 (72%), Positives = 241/283 (85%), Gaps = 1/283 (0%)
 Frame = +2

Query: 53   KDEVRRLATRMDLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGILLEAETGDFLGKHH 232
            KDEVR LAT++DLPN+DRKDSQGICFLGKIKFSEFVARHIGE EGI+LEAETGD LGKH 
Sbjct: 260  KDEVRSLATKLDLPNRDRKDSQGICFLGKIKFSEFVARHIGEKEGIILEAETGDLLGKHR 319

Query: 233  GFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKQRRRFSVGSMKWLSGSP 412
            GFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFS DK+RR F VGS+KW+S  P
Sbjct: 320  GFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSLDKRRRLFRVGSLKWISREP 379

Query: 413  PDQVDQLQCKVRHGPSFYDCSLTMEPGEDNQGDSFIVHISEDDQGLAAGQFTAFYQGKTC 592
            P Q++QLQCKVRHGP FY+CS TMEPGED   D  +V +SEDDQGLAAGQFTAFYQG++C
Sbjct: 380  PGQINQLQCKVRHGPGFYNCSFTMEPGEDGHEDVAVVQLSEDDQGLAAGQFTAFYQGRSC 439

Query: 593  LGSGVIVESKDDEGFPVCARAMEIARMEDKSKLGKPIKIKVKPDSSPKNSELKETVELHR 772
            +GSGVI+ES DD+GFPVCA+A+EIARMEDKSKLGKP+KIKV+P++ PK S+  + ++L R
Sbjct: 440  IGSGVILESWDDQGFPVCAKALEIARMEDKSKLGKPVKIKVRPETPPKGSDQIDGIKLDR 499

Query: 773  ESVSPQ-LINNDVIDSQTCASRGLFSSIPMNWLQKLREKWMQI 898
            E  + Q  + +++  S    +R  F   PMNWLQK+REKW+Q+
Sbjct: 500  ELANSQAAVTDEISRSSEGEARSRF---PMNWLQKIREKWLQM 539


>ref|XP_006470603.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like [Citrus
            sinensis]
          Length = 525

 Score =  414 bits (1064), Expect = e-113
 Identities = 201/283 (71%), Positives = 233/283 (82%)
 Frame = +2

Query: 50   SKDEVRRLATRMDLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGILLEAETGDFLGKH 229
            SK+EVR+ AT+ DLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEG++LEAETGDFLGKH
Sbjct: 245  SKEEVRKFATQFDLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGVILEAETGDFLGKH 304

Query: 230  HGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKQRRRFSVGSMKWLSGS 409
             GFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNY+SFDK+RR F VGS+KWLSG 
Sbjct: 305  WGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYYSFDKKRRLFRVGSLKWLSGL 364

Query: 410  PPDQVDQLQCKVRHGPSFYDCSLTMEPGEDNQGDSFIVHISEDDQGLAAGQFTAFYQGKT 589
            PP+++ +LQCKVRHGP FY+C+L +E  ED   +  +V +SEDDQGLAAGQF AFYQG  
Sbjct: 365  PPEEISRLQCKVRHGPGFYNCNLMIEHVEDGGENIAVVQLSEDDQGLAAGQFAAFYQGTR 424

Query: 590  CLGSGVIVESKDDEGFPVCARAMEIARMEDKSKLGKPIKIKVKPDSSPKNSELKETVELH 769
            C+GSGVI+ES DD+GFPVC +A+EIARMEDKSKLGKPIKIKVKP+ +P+     + V+  
Sbjct: 425  CIGSGVILESWDDQGFPVCEKALEIARMEDKSKLGKPIKIKVKPE-TPEEFNSNKGVQSS 483

Query: 770  RESVSPQLINNDVIDSQTCASRGLFSSIPMNWLQKLREKWMQI 898
            R  V+ Q  N+    S T +     S  PMNWLQKLREKW +I
Sbjct: 484  RNLVNAQ--NSIAEKSNTTSQEEAISRFPMNWLQKLREKWPRI 524


>gb|EXB93298.1| tRNA-specific 2-thiouridylase mnmA [Morus notabilis]
          Length = 580

 Score =  411 bits (1056), Expect = e-112
 Identities = 201/285 (70%), Positives = 228/285 (80%), Gaps = 1/285 (0%)
 Frame = +2

Query: 50   SKDEVRRLATRMDLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGILLEAETGDFLGKH 229
            SK+EVR+LA + DLPNKDRKDSQGICFLGKIKFSEFVARHIGE EGI+LEAETGDFLG H
Sbjct: 305  SKEEVRKLAKKFDLPNKDRKDSQGICFLGKIKFSEFVARHIGEKEGIILEAETGDFLGMH 364

Query: 230  HGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKQRRRFSVGSMKWLSGS 409
             GFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVF SRNYFSFDK+RR F VGS+KWL+G 
Sbjct: 365  RGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFASRNYFSFDKRRRIFRVGSLKWLNGL 424

Query: 410  PPDQVDQLQCKVRHGPSFYDCSLTMEPGEDNQGDSFIVHISEDDQGLAAGQFTAFYQGKT 589
            PPDQ  QLQCKVRHGP FY+CS TME GE++  D  +V +SEDDQGLA GQF AFYQGK+
Sbjct: 425  PPDQTCQLQCKVRHGPGFYNCSFTMESGENSGEDVAVVQLSEDDQGLAVGQFAAFYQGKS 484

Query: 590  CLGSGVIVESKDDEGFPVCARAMEIARMEDKSKLGKPIKIKVKPDSSPKNSELKETVELH 769
            CLGSG+I+ES DD+GFPVC +A+EIA+MEDKS LGKP+KIKVKP+S       K + EL 
Sbjct: 485  CLGSGIILESWDDQGFPVCEKALEIAKMEDKSLLGKPVKIKVKPESPTIEPGQKSSTELD 544

Query: 770  RESVSPQLINNDVIDSQTCASRGLFS-SIPMNWLQKLREKWMQIF 901
            R   S  +           A +G  + S PM WLQ+LREKW +IF
Sbjct: 545  RGQRSSHI---------AAAKQGRQALSFPMKWLQRLREKWFRIF 580


>ref|XP_007209892.1| hypothetical protein PRUPE_ppa004048mg [Prunus persica]
            gi|462405627|gb|EMJ11091.1| hypothetical protein
            PRUPE_ppa004048mg [Prunus persica]
          Length = 533

 Score =  405 bits (1042), Expect = e-111
 Identities = 198/285 (69%), Positives = 229/285 (80%), Gaps = 1/285 (0%)
 Frame = +2

Query: 50   SKDEVRRLATRMDLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGILLEAETGDFLGKH 229
            SKDEVR+LAT+ DL NK RKDSQGICFLGK+KFSEF+ARHIGE EGI+LEAETGDFLG H
Sbjct: 252  SKDEVRKLATKFDLLNKQRKDSQGICFLGKLKFSEFIARHIGEKEGIILEAETGDFLGTH 311

Query: 230  HGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKQRRRFSVGSMKWLSGS 409
             GFWFYTIGQRQGLRLPGGPWYVVEKD+KNNVVFVSRNYFS DK+RR F VGS+KWL+G 
Sbjct: 312  RGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSVDKRRRLFRVGSLKWLNGF 371

Query: 410  PPDQVDQLQCKVRHGPSFYDCSLTMEPGEDNQGDSFIVHISEDDQGLAAGQFTAFYQGKT 589
            P +Q+ QLQCKVRHGP F +CSL MEPG     D  +VH+SEDDQGLAAGQF AFYQG+T
Sbjct: 372  PQNQISQLQCKVRHGPGFRNCSLIMEPGAYGHEDVAVVHLSEDDQGLAAGQFAAFYQGRT 431

Query: 590  CLGSGVIVESKDDEGFPVCARAMEIARMEDKSKLGKPIKIKVKPDSSPKNSELKETVELH 769
            CLGSGVI+ES DD+GFPVCARA+EIARMEDKS LGKP+KIKVKP++    S+  + +EL 
Sbjct: 432  CLGSGVILESWDDQGFPVCARALEIARMEDKSLLGKPVKIKVKPENPITESDQVDGMELC 491

Query: 770  RESVSPQLINNDVIDSQTCASRG-LFSSIPMNWLQKLREKWMQIF 901
            R      +        +   S+G   +  P+NWLQK REKW++IF
Sbjct: 492  RGLKHSGI---SAAKQRMQVSQGEAITRFPVNWLQKFREKWLRIF 533


>ref|XP_004142116.1| PREDICTED: tRNA-specific 2-thiouridylase MnmA-like [Cucumis sativus]
            gi|449524848|ref|XP_004169433.1| PREDICTED: tRNA-specific
            2-thiouridylase MnmA-like [Cucumis sativus]
          Length = 538

 Score =  405 bits (1042), Expect = e-111
 Identities = 195/283 (68%), Positives = 232/283 (81%)
 Frame = +2

Query: 53   KDEVRRLATRMDLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGILLEAETGDFLGKHH 232
            KDEVR+LA + +LPNKDRKDSQGICFLGKIKFSEFVARHIGE EG++LEAE+GDFLGKH 
Sbjct: 258  KDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHR 317

Query: 233  GFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKQRRRFSVGSMKWLSGSP 412
            GFWFYTIGQRQGLRLPGGPWYVVEKD+KNNVVFVSRNYFSFDK+RR F VGS+KWLSG P
Sbjct: 318  GFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGFP 377

Query: 413  PDQVDQLQCKVRHGPSFYDCSLTMEPGEDNQGDSFIVHISEDDQGLAAGQFTAFYQGKTC 592
            PDQ  +LQCKVRHGPSFY C+LTME  ED   D  +VH+SEDDQGLAAGQF AFY+G++C
Sbjct: 378  PDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSC 437

Query: 593  LGSGVIVESKDDEGFPVCARAMEIARMEDKSKLGKPIKIKVKPDSSPKNSELKETVELHR 772
            +GSGVI+ES DD+GFPVC +A+E A+MEDKSKLGKPIKIKVKP+S  K S+ K+  E+H 
Sbjct: 438  IGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKPESGMKESKAKDDTEIHD 497

Query: 773  ESVSPQLINNDVIDSQTCASRGLFSSIPMNWLQKLREKWMQIF 901
            +  + +    + I  +  +     ++  M WL  L++K +QIF
Sbjct: 498  KLRNEEATVVEQI--REVSPDEAMATPSMKWLGSLKKKLLQIF 538


>ref|XP_004507093.1| PREDICTED: tRNA-specific 2-thiouridylase MnmA-like [Cicer
           arietinum]
          Length = 484

 Score =  394 bits (1012), Expect = e-107
 Identities = 186/225 (82%), Positives = 203/225 (90%)
 Frame = +2

Query: 53  KDEVRRLATRMDLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGILLEAETGDFLGKHH 232
           KDEVRRLAT+ DLPNKDRKDSQGICFLGKIKFSEFVARHIGE EGI+LEAETGDFLGKH 
Sbjct: 245 KDEVRRLATKFDLPNKDRKDSQGICFLGKIKFSEFVARHIGEKEGIILEAETGDFLGKHR 304

Query: 233 GFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKQRRRFSVGSMKWLSGSP 412
           GFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFS DK+RR F VGS+KWLSG P
Sbjct: 305 GFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSIDKRRRVFRVGSLKWLSGLP 364

Query: 413 PDQVDQLQCKVRHGPSFYDCSLTMEPGEDNQGDSFIVHISEDDQGLAAGQFTAFYQGKTC 592
           P Q  QL+CKVRHGP FYDCSL ME   +N  DS +V ISEDDQGLAAGQFTAFY+G+TC
Sbjct: 365 PSQTSQLRCKVRHGPGFYDCSLQMELEGENPIDSAVVRISEDDQGLAAGQFTAFYEGRTC 424

Query: 593 LGSGVIVESKDDEGFPVCARAMEIARMEDKSKLGKPIKIKVKPDS 727
           +GSGVI+ES DD+GFPVC +A+EIARMEDKSK+G P+KIKVKPD+
Sbjct: 425 IGSGVILESWDDQGFPVCTKALEIARMEDKSKIGNPVKIKVKPDN 469


>ref|XP_002298404.2| hypothetical protein POPTR_0001s26620g [Populus trichocarpa]
            gi|550348245|gb|EEE83209.2| hypothetical protein
            POPTR_0001s26620g [Populus trichocarpa]
          Length = 512

 Score =  392 bits (1007), Expect = e-106
 Identities = 183/247 (74%), Positives = 213/247 (86%)
 Frame = +2

Query: 53   KDEVRRLATRMDLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGILLEAETGDFLGKHH 232
            K+EVR LA++ DLPNKDRKDSQGICFLGKIKFSEFV RHIGE EG++LEAETGDFLG H 
Sbjct: 262  KEEVRNLASKFDLPNKDRKDSQGICFLGKIKFSEFVGRHIGESEGVILEAETGDFLGIHR 321

Query: 233  GFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKQRRRFSVGSMKWLSGSP 412
            GFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFS DK+RR F VGS+KWLSGSP
Sbjct: 322  GFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSLDKRRRSFRVGSLKWLSGSP 381

Query: 413  PDQVDQLQCKVRHGPSFYDCSLTMEPGEDNQGDSFIVHISEDDQGLAAGQFTAFYQGKTC 592
             D   QLQCKVRHGP+FYDCS+ +E GED+  +   VH+ EDDQGLAAGQFTAFYQG+TC
Sbjct: 382  TDMTTQLQCKVRHGPTFYDCSVKIELGEDSPEEVVAVHLCEDDQGLAAGQFTAFYQGRTC 441

Query: 593  LGSGVIVESKDDEGFPVCARAMEIARMEDKSKLGKPIKIKVKPDSSPKNSELKETVELHR 772
            +GSGVI+ES DD+GFPVC +A+E+AR+EDKSKLGKP+KIKVKP++S + S  K+ +E  +
Sbjct: 442  IGSGVILESWDDKGFPVCQKALELARLEDKSKLGKPVKIKVKPETSDQESVQKDGIEPDK 501

Query: 773  ESVSPQL 793
                P++
Sbjct: 502  GLAEPRV 508


>ref|XP_006850208.1| hypothetical protein AMTR_s00022p00251260 [Amborella trichopoda]
            gi|548853806|gb|ERN11789.1| hypothetical protein
            AMTR_s00022p00251260 [Amborella trichopoda]
          Length = 565

 Score =  388 bits (996), Expect = e-105
 Identities = 182/284 (64%), Positives = 222/284 (78%)
 Frame = +2

Query: 50   SKDEVRRLATRMDLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGILLEAETGDFLGKH 229
            SKDEVRRLA +M LPNKDRKDSQGICFLGK+KFS+FV RHIGEMEG+LLEAETGDFLGKH
Sbjct: 299  SKDEVRRLAAKMGLPNKDRKDSQGICFLGKLKFSDFVTRHIGEMEGVLLEAETGDFLGKH 358

Query: 230  HGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKQRRRFSVGSMKWLSGS 409
             G+WFYTIGQRQGLRLPGGPWYVV KD++NNV+FVSRNYFSFDK+RR F VGS+KWLSG+
Sbjct: 359  QGYWFYTIGQRQGLRLPGGPWYVVNKDIENNVIFVSRNYFSFDKKRRSFRVGSLKWLSGA 418

Query: 410  PPDQVDQLQCKVRHGPSFYDCSLTMEPGEDNQGDSFIVHISEDDQGLAAGQFTAFYQGKT 589
            PP+ +DQLQCKVRHGP FYDC+  +E   D   DS ++HISEDDQGLAAGQF AFY+   
Sbjct: 419  PPEHLDQLQCKVRHGPCFYDCTFKIESRCDEHEDSALIHISEDDQGLAAGQFAAFYRKNV 478

Query: 590  CLGSGVIVESKDDEGFPVCARAMEIARMEDKSKLGKPIKIKVKPDSSPKNSELKETVELH 769
            C+GSG+I+ES DD+ F VC++A+E A+MEDKSKLG PIKIKVK + +PK  + K+   L 
Sbjct: 479  CIGSGIILESWDDQNFSVCSKALERAKMEDKSKLGLPIKIKVKSEPNPKGFDCKDNELLL 538

Query: 770  RESVSPQLINNDVIDSQTCASRGLFSSIPMNWLQKLREKWMQIF 901
             E  + + +                 S+ M+W Q+L+ +W+ +F
Sbjct: 539  AEPKTDEKL-----------------SVAMDWFQRLKTQWLHLF 565


>ref|XP_006590965.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like
           isoform X2 [Glycine max]
          Length = 392

 Score =  387 bits (994), Expect = e-105
 Identities = 182/225 (80%), Positives = 199/225 (88%)
 Frame = +2

Query: 53  KDEVRRLATRMDLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGILLEAETGDFLGKHH 232
           KDEVR+LAT+ DLPNKDRKDSQGICFLGKI+FSEFVARHIGE EG++LEAETGDFLGKH 
Sbjct: 142 KDEVRKLATKFDLPNKDRKDSQGICFLGKIRFSEFVARHIGEREGVILEAETGDFLGKHR 201

Query: 233 GFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKQRRRFSVGSMKWLSGSP 412
           GFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDK+RR F VGS KWLSG P
Sbjct: 202 GFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKRRRVFRVGSFKWLSGLP 261

Query: 413 PDQVDQLQCKVRHGPSFYDCSLTMEPGEDNQGDSFIVHISEDDQGLAAGQFTAFYQGKTC 592
           P Q  QLQCKVRHGP FYDCSL ME   D Q  S +V ISEDDQGLAAGQF AFY+G+TC
Sbjct: 262 PGQTTQLQCKVRHGPGFYDCSLQMEVEADGQCHSAVVRISEDDQGLAAGQFAAFYEGRTC 321

Query: 593 LGSGVIVESKDDEGFPVCARAMEIARMEDKSKLGKPIKIKVKPDS 727
           +GSGVI+E  DD+ FPVC +A+EIARMEDKSK+G P+KIKVKPD+
Sbjct: 322 IGSGVILEFWDDQSFPVCTKALEIARMEDKSKIGNPVKIKVKPDN 366


>ref|XP_003539065.2| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like
           isoform X1 [Glycine max]
          Length = 502

 Score =  387 bits (994), Expect = e-105
 Identities = 182/225 (80%), Positives = 199/225 (88%)
 Frame = +2

Query: 53  KDEVRRLATRMDLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGILLEAETGDFLGKHH 232
           KDEVR+LAT+ DLPNKDRKDSQGICFLGKI+FSEFVARHIGE EG++LEAETGDFLGKH 
Sbjct: 252 KDEVRKLATKFDLPNKDRKDSQGICFLGKIRFSEFVARHIGEREGVILEAETGDFLGKHR 311

Query: 233 GFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKQRRRFSVGSMKWLSGSP 412
           GFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDK+RR F VGS KWLSG P
Sbjct: 312 GFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKRRRVFRVGSFKWLSGLP 371

Query: 413 PDQVDQLQCKVRHGPSFYDCSLTMEPGEDNQGDSFIVHISEDDQGLAAGQFTAFYQGKTC 592
           P Q  QLQCKVRHGP FYDCSL ME   D Q  S +V ISEDDQGLAAGQF AFY+G+TC
Sbjct: 372 PGQTTQLQCKVRHGPGFYDCSLQMEVEADGQCHSAVVRISEDDQGLAAGQFAAFYEGRTC 431

Query: 593 LGSGVIVESKDDEGFPVCARAMEIARMEDKSKLGKPIKIKVKPDS 727
           +GSGVI+E  DD+ FPVC +A+EIARMEDKSK+G P+KIKVKPD+
Sbjct: 432 IGSGVILEFWDDQSFPVCTKALEIARMEDKSKIGNPVKIKVKPDN 476


>ref|XP_007132017.1| hypothetical protein PHAVU_011G059600g [Phaseolus vulgaris]
           gi|561005017|gb|ESW04011.1| hypothetical protein
           PHAVU_011G059600g [Phaseolus vulgaris]
          Length = 488

 Score =  386 bits (992), Expect = e-105
 Identities = 185/225 (82%), Positives = 197/225 (87%)
 Frame = +2

Query: 53  KDEVRRLATRMDLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGILLEAETGDFLGKHH 232
           KDEVRRLAT  DLPNKDRKDSQGICFLGKI+FSEFVARHIGE EGI+LEAETGDFLGKH 
Sbjct: 250 KDEVRRLATEFDLPNKDRKDSQGICFLGKIRFSEFVARHIGEREGIILEAETGDFLGKHR 309

Query: 233 GFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKQRRRFSVGSMKWLSGSP 412
           GFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDK+RR F VGS KWLS  P
Sbjct: 310 GFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKRRRVFRVGSFKWLSELP 369

Query: 413 PDQVDQLQCKVRHGPSFYDCSLTMEPGEDNQGDSFIVHISEDDQGLAAGQFTAFYQGKTC 592
           P Q  QLQCKVRHGP FYDCSL ME   D QG S +V ISEDDQGLAAGQF AFY+G+TC
Sbjct: 370 PGQTSQLQCKVRHGPGFYDCSLQMEVEGDGQGHSAVVRISEDDQGLAAGQFAAFYEGRTC 429

Query: 593 LGSGVIVESKDDEGFPVCARAMEIARMEDKSKLGKPIKIKVKPDS 727
           LGSGVI+E  D++ FPVC +A+EIARMEDKS LG P+KIKVKPDS
Sbjct: 430 LGSGVILEFWDEQSFPVCTKALEIARMEDKSILGNPVKIKVKPDS 474


>ref|XP_007132016.1| hypothetical protein PHAVU_011G059600g [Phaseolus vulgaris]
           gi|561005016|gb|ESW04010.1| hypothetical protein
           PHAVU_011G059600g [Phaseolus vulgaris]
          Length = 380

 Score =  386 bits (992), Expect = e-105
 Identities = 185/225 (82%), Positives = 197/225 (87%)
 Frame = +2

Query: 53  KDEVRRLATRMDLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGILLEAETGDFLGKHH 232
           KDEVRRLAT  DLPNKDRKDSQGICFLGKI+FSEFVARHIGE EGI+LEAETGDFLGKH 
Sbjct: 142 KDEVRRLATEFDLPNKDRKDSQGICFLGKIRFSEFVARHIGEREGIILEAETGDFLGKHR 201

Query: 233 GFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKQRRRFSVGSMKWLSGSP 412
           GFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDK+RR F VGS KWLS  P
Sbjct: 202 GFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKRRRVFRVGSFKWLSELP 261

Query: 413 PDQVDQLQCKVRHGPSFYDCSLTMEPGEDNQGDSFIVHISEDDQGLAAGQFTAFYQGKTC 592
           P Q  QLQCKVRHGP FYDCSL ME   D QG S +V ISEDDQGLAAGQF AFY+G+TC
Sbjct: 262 PGQTSQLQCKVRHGPGFYDCSLQMEVEGDGQGHSAVVRISEDDQGLAAGQFAAFYEGRTC 321

Query: 593 LGSGVIVESKDDEGFPVCARAMEIARMEDKSKLGKPIKIKVKPDS 727
           LGSGVI+E  D++ FPVC +A+EIARMEDKS LG P+KIKVKPDS
Sbjct: 322 LGSGVILEFWDEQSFPVCTKALEIARMEDKSILGNPVKIKVKPDS 366


>ref|XP_002513510.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase,
           putative [Ricinus communis] gi|223547418|gb|EEF48913.1|
           tRNA
           (5-methylaminomethyl-2-thiouridylate)-methyltransferase,
           putative [Ricinus communis]
          Length = 497

 Score =  386 bits (992), Expect = e-105
 Identities = 180/243 (74%), Positives = 211/243 (86%)
 Frame = +2

Query: 44  GDSKDEVRRLATRMDLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGILLEAETGDFLG 223
           G SKDEVR+LAT  +LPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGI+LEAETGDFLG
Sbjct: 244 GISKDEVRKLATLFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGIMLEAETGDFLG 303

Query: 224 KHHGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKQRRRFSVGSMKWLS 403
            H GFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDK+RR   VGS++W +
Sbjct: 304 NHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKRRRLLRVGSLRWFN 363

Query: 404 GSPPDQVDQLQCKVRHGPSFYDCSLTMEPGEDNQGDSFIVHISEDDQGLAAGQFTAFYQG 583
           GSPP +  +LQCKVRHGP FY+CS+ +EPGE +  D  IV + EDDQGLAAGQF AFY+G
Sbjct: 364 GSPPKETSELQCKVRHGPGFYNCSVVIEPGEVDDQDVAIVQLCEDDQGLAAGQFAAFYKG 423

Query: 584 KTCLGSGVIVESKDDEGFPVCARAMEIARMEDKSKLGKPIKIKVKPDSSPKNSELKETVE 763
           +TC+GSGVI+ES +D+GFPVC RA+E+AR+EDKS+LGKP+KIKVKP+   +  + K+ +E
Sbjct: 424 RTCIGSGVILESWNDQGFPVCERALELARIEDKSELGKPVKIKVKPEIPVREFDGKDGIE 483

Query: 764 LHR 772
           + R
Sbjct: 484 VER 486


>ref|XP_004301461.1| PREDICTED: tRNA-specific 2-thiouridylase MnmA-like [Fragaria vesca
           subsp. vesca]
          Length = 475

 Score =  367 bits (942), Expect = 3e-99
 Identities = 168/226 (74%), Positives = 198/226 (87%)
 Frame = +2

Query: 50  SKDEVRRLATRMDLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGILLEAETGDFLGKH 229
           SKD+VR+LAT+ DLPNK+RKDSQGICFLGK++FSEF+ +HIGE EG++LEAETGD LGKH
Sbjct: 249 SKDKVRKLATKFDLPNKERKDSQGICFLGKVRFSEFIGKHIGEKEGVILEAETGDLLGKH 308

Query: 230 HGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKQRRRFSVGSMKWLSGS 409
            GFWFYTIGQRQGLRLPGGPWYVVEKD+K+NVVFVSRNY+S DK+RR F VGS+KWL GS
Sbjct: 309 RGFWFYTIGQRQGLRLPGGPWYVVEKDIKHNVVFVSRNYYSVDKRRRLFRVGSLKWLHGS 368

Query: 410 PPDQVDQLQCKVRHGPSFYDCSLTMEPGEDNQGDSFIVHISEDDQGLAAGQFTAFYQGKT 589
           PP Q+ QLQCKVRHGP F +C++ +E GED   D  +V +SEDDQGLAAGQF AFYQG+T
Sbjct: 369 PPAQISQLQCKVRHGPRFCNCTVKIEHGEDGNEDVAVVSLSEDDQGLAAGQFAAFYQGRT 428

Query: 590 CLGSGVIVESKDDEGFPVCARAMEIARMEDKSKLGKPIKIKVKPDS 727
           CLGSGVI+ES DD+GFPVC +A+E ARMEDKS LGKP+KIK  P++
Sbjct: 429 CLGSGVILESWDDQGFPVCTKALENARMEDKSLLGKPVKIKFIPEA 474


>ref|XP_006393065.1| hypothetical protein EUTSA_v10011432mg [Eutrema salsugineum]
           gi|557089643|gb|ESQ30351.1| hypothetical protein
           EUTSA_v10011432mg [Eutrema salsugineum]
          Length = 488

 Score =  364 bits (935), Expect = 2e-98
 Identities = 172/235 (73%), Positives = 196/235 (83%)
 Frame = +2

Query: 53  KDEVRRLATRMDLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGILLEAETGDFLGKHH 232
           K+EVR+LAT+ DLPNKDRKDSQGICFLGKIKFS+FV RHIGEMEGI+LEAETGDFLG H 
Sbjct: 249 KEEVRKLATKFDLPNKDRKDSQGICFLGKIKFSDFVGRHIGEMEGIILEAETGDFLGNHR 308

Query: 233 GFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKQRRRFSVGSMKWLSGSP 412
           GFWFYTIGQRQGLRLPGGPWYVVEKD KNNVVFVSRNY+S DK+RR F VGS++WLSG P
Sbjct: 309 GFWFYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYYSIDKRRRIFRVGSLRWLSGKP 368

Query: 413 PDQVDQLQCKVRHGPSFYDCSLTMEPGEDNQGDSFIVHISEDDQGLAAGQFTAFYQGKTC 592
              V QL+CKVRHGP FY CS  ME     QGD  +VH+ EDDQGLAAGQF AFY+G TC
Sbjct: 369 SGNVSQLRCKVRHGPGFYSCSFEMEA----QGDVAVVHLDEDDQGLAAGQFAAFYEGTTC 424

Query: 593 LGSGVIVESKDDEGFPVCARAMEIARMEDKSKLGKPIKIKVKPDSSPKNSELKET 757
           +GSGVI+ES DD+ FPVCA+A+E+A MEDK+KLGKP+KI   P +    +E +ET
Sbjct: 425 IGSGVILESWDDQCFPVCAKALELAAMEDKTKLGKPVKIMTMPVTPSVETEPRET 479


>ref|XP_006358437.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like
           [Solanum tuberosum]
          Length = 488

 Score =  362 bits (930), Expect = 9e-98
 Identities = 170/226 (75%), Positives = 196/226 (86%)
 Frame = +2

Query: 53  KDEVRRLATRMDLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGILLEAETGDFLGKHH 232
           KDEVR LA   +LPN+DRKDSQGICFLGKIKFSEFVARHIGE EGI+LEAE GD+LG H 
Sbjct: 260 KDEVRTLAKSFNLPNQDRKDSQGICFLGKIKFSEFVARHIGESEGIILEAENGDYLGNHR 319

Query: 233 GFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKQRRRFSVGSMKWLSGSP 412
           GFWFYTIGQRQGLRLPGGPWYVVEKD+KNNVV+VSRNYFS DK+RR F VGS+KWLSG  
Sbjct: 320 GFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVYVSRNYFSVDKKRRLFRVGSLKWLSGLF 379

Query: 413 PDQVDQLQCKVRHGPSFYDCSLTMEPGEDNQGDSFIVHISEDDQGLAAGQFTAFYQGKTC 592
           P Q+++LQCKVRHGPSFYDCSL ME  +  Q +  +V +S DDQGLAAGQF AFY G+TC
Sbjct: 380 PKQINELQCKVRHGPSFYDCSLVMEVDQHGQ-EVAVVRLSGDDQGLAAGQFAAFYNGRTC 438

Query: 593 LGSGVIVESKDDEGFPVCARAMEIARMEDKSKLGKPIKIKVKPDSS 730
           +GSG+I+ES DD+G+P+C +A+EIARMEDKSKLGKP+KI VKP+ S
Sbjct: 439 IGSGIILESWDDQGYPICEKALEIARMEDKSKLGKPVKIMVKPELS 484


>ref|XP_002894309.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase
           [Arabidopsis lyrata subsp. lyrata]
           gi|297340151|gb|EFH70568.1| tRNA
           (5-methylaminomethyl-2-thiouridylate)-methyltransferase
           [Arabidopsis lyrata subsp. lyrata]
          Length = 500

 Score =  357 bits (917), Expect = 3e-96
 Identities = 167/223 (74%), Positives = 190/223 (85%)
 Frame = +2

Query: 53  KDEVRRLATRMDLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGILLEAETGDFLGKHH 232
           KDEVR+LAT+ DLPNKDRKDSQGICFLGKIKFS+FV  HIGEMEGI+LEAETGDFLG H 
Sbjct: 261 KDEVRKLATQFDLPNKDRKDSQGICFLGKIKFSDFVCAHIGEMEGIILEAETGDFLGNHR 320

Query: 233 GFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKQRRRFSVGSMKWLSGSP 412
           GFWFYTIGQRQGLRLPGGPWYVVEKD KNNVVFVSRNY+S DK+RR F VGS++WLSG P
Sbjct: 321 GFWFYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYYSIDKRRRIFRVGSLRWLSGKP 380

Query: 413 PDQVDQLQCKVRHGPSFYDCSLTMEPGEDNQGDSFIVHISEDDQGLAAGQFTAFYQGKTC 592
              V +L+CKVRHGPSFY CS  ME      GD  +VH+ EDDQGLAAGQF AFY+G TC
Sbjct: 381 SGNVRELRCKVRHGPSFYSCSFEMEA----DGDVAVVHLDEDDQGLAAGQFAAFYEGTTC 436

Query: 593 LGSGVIVESKDDEGFPVCARAMEIARMEDKSKLGKPIKIKVKP 721
           +GSGVI+ES DD+ FPVCA+A+++A MEDK+KLGKP+KI+  P
Sbjct: 437 IGSGVILESWDDQCFPVCAKALQLAAMEDKTKLGKPVKIRTMP 479


>ref|XP_006280360.1| hypothetical protein CARUB_v10026287mg [Capsella rubella]
           gi|482549064|gb|EOA13258.1| hypothetical protein
           CARUB_v10026287mg [Capsella rubella]
          Length = 494

 Score =  357 bits (915), Expect = 5e-96
 Identities = 167/234 (71%), Positives = 194/234 (82%)
 Frame = +2

Query: 53  KDEVRRLATRMDLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGILLEAETGDFLGKHH 232
           KDEVR+LAT+ DLPNKDRKDSQGICFLGKIKFS+FV  HIGEMEG++LEAETGDFLG H 
Sbjct: 255 KDEVRKLATQFDLPNKDRKDSQGICFLGKIKFSDFVGTHIGEMEGVILEAETGDFLGNHR 314

Query: 233 GFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKQRRRFSVGSMKWLSGSP 412
           GFWFYTIGQRQGLRLPGGPWYVVEKD KNNVVFVSRNY+S DK+RR F VGS++WL G P
Sbjct: 315 GFWFYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYYSIDKKRRIFRVGSLRWLCGKP 374

Query: 413 PDQVDQLQCKVRHGPSFYDCSLTMEPGEDNQGDSFIVHISEDDQGLAAGQFTAFYQGKTC 592
            D V +L+CKVRHGP FY CS  ME     +GD  +VH+ EDDQGLAAGQF AFY+G TC
Sbjct: 375 SDNVSKLRCKVRHGPGFYSCSFEMEA----KGDVAVVHLDEDDQGLAAGQFAAFYEGTTC 430

Query: 593 LGSGVIVESKDDEGFPVCARAMEIARMEDKSKLGKPIKIKVKPDSSPKNSELKE 754
           +GSGVI+ES DD+ FPVCA+A+++A MEDK+KLGKP+KI   P +S   ++  E
Sbjct: 431 IGSGVILESWDDQCFPVCAKALQLAAMEDKTKLGKPVKILTMPVTSSVEADSGE 484


>ref|XP_004247484.1| PREDICTED: tRNA-specific 2-thiouridylase MnmA-like [Solanum
           lycopersicum]
          Length = 489

 Score =  356 bits (914), Expect = 6e-96
 Identities = 167/228 (73%), Positives = 194/228 (85%)
 Frame = +2

Query: 53  KDEVRRLATRMDLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGILLEAETGDFLGKHH 232
           KDEVR LA   +LPN++RKDSQGICFLGKIKFSEFVARHIGE EGI+LEAE GD+LG H 
Sbjct: 256 KDEVRMLAKSFNLPNQERKDSQGICFLGKIKFSEFVARHIGESEGIILEAENGDYLGNHR 315

Query: 233 GFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKQRRRFSVGSMKWLSGSP 412
           GFWFYTIGQRQGLRLPGGPWYVVEKD+KNNVV+VSRNYFS DK+RR F VGS+KWLSG  
Sbjct: 316 GFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVYVSRNYFSVDKKRRLFRVGSLKWLSGLF 375

Query: 413 PDQVDQLQCKVRHGPSFYDCSLTMEPGEDNQGDSFIVHISEDDQGLAAGQFTAFYQGKTC 592
           P Q+D+LQCKVRHGP FY+CSL ME  +  Q +  +V +S DDQGLAAGQF AFY G+TC
Sbjct: 376 PKQIDELQCKVRHGPGFYNCSLVMEVDQHGQ-EVAVVRLSGDDQGLAAGQFAAFYDGRTC 434

Query: 593 LGSGVIVESKDDEGFPVCARAMEIARMEDKSKLGKPIKIKVKPDSSPK 736
           +GSG+I+ES DD+G+P+C RA+EIARMEDKSKLGKP+KI ++   S K
Sbjct: 435 IGSGIILESWDDQGYPICERALEIARMEDKSKLGKPVKIMLEEQQSDK 482