BLASTX nr result

ID: Akebia23_contig00022856 seq

BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Akebia23_contig00022856
         (1057 letters)

Database: ./nr 
           38,876,450 sequences; 13,856,398,315 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002284042.2| PREDICTED: isoamylase 3, chloroplastic-like ...   428   e-117
emb|CBI19205.3| unnamed protein product [Vitis vinifera]              428   e-117
ref|XP_007018697.1| Isoamylase 3 [Theobroma cacao] gi|508724025|...   424   e-116
ref|XP_006472546.1| PREDICTED: isoamylase 3, chloroplastic-like ...   424   e-116
ref|XP_006433917.1| hypothetical protein CICLE_v10000347mg [Citr...   422   e-116
ref|XP_006433916.1| hypothetical protein CICLE_v10000347mg [Citr...   422   e-116
ref|XP_003527894.1| PREDICTED: isoamylase 3, chloroplastic-like ...   422   e-115
ref|XP_003522781.1| PREDICTED: isoamylase 3, chloroplastic-like ...   419   e-114
ref|XP_006578313.1| PREDICTED: isoamylase 3, chloroplastic-like ...   418   e-114
ref|XP_007136280.1| hypothetical protein PHAVU_009G033200g [Phas...   417   e-114
ref|XP_002513977.1| isoamylase, putative [Ricinus communis] gi|2...   416   e-113
ref|XP_002301076.2| isoamylase family protein [Populus trichocar...   410   e-112
ref|XP_004501686.1| PREDICTED: isoamylase 3, chloroplastic-like ...   407   e-111
ref|XP_006578312.1| PREDICTED: isoamylase 3, chloroplastic-like ...   399   e-108
ref|XP_004299680.1| PREDICTED: isoamylase 3, chloroplastic-like ...   398   e-108
ref|XP_004501687.1| PREDICTED: isoamylase 3, chloroplastic-like ...   396   e-108
gb|AAZ81837.1| isoamylase isoform 3 [Pisum sativum]                   394   e-107
ref|XP_004501688.1| PREDICTED: isoamylase 3, chloroplastic-like ...   389   e-105
ref|XP_002874494.1| ATISA3/ISA3 [Arabidopsis lyrata subsp. lyrat...   384   e-104
ref|NP_001275220.1| isoamylase isoform 3 [Solanum tuberosum] gi|...   383   e-104

>ref|XP_002284042.2| PREDICTED: isoamylase 3, chloroplastic-like [Vitis vinifera]
          Length = 784

 Score =  428 bits (1101), Expect = e-117
 Identities = 216/334 (64%), Positives = 251/334 (75%)
 Frame = -2

Query: 1002 MDALLYSQHGTQMLHLNSHFCDSKTKLAPLSSRNPTRCASIEGFRLFTLRLRSSKKSVFG 823
            MD  L S    +M+H      ++K  L    S   +  +S    R+  + L  SKK V G
Sbjct: 1    MDVFLSSPKTPKMIHPLVGESNAKFPLFSALSNRDSGASSFGTSRIIGMGLLLSKK-VSG 59

Query: 822  SNEQTFISKVRESDRINRKFTSVYNRRAQESVIEEEAPEMSEISGPFKVSPGQAFPLGAS 643
            SN      K    D +  + T+ Y R AQ+ V+EEEAP+++E S   KV PGQAFPLG S
Sbjct: 60   SN-----MKRTTWDCLRPRTTNAYGRHAQDRVLEEEAPQIAETSPSLKVLPGQAFPLGVS 114

Query: 642  EVENGINFSLFSRHASAVTLCLTIPERGNHEELDSGMVELTLDPSINKTGDIWHICVEDL 463
            EVENGINF++FS+HA+A+ LCL +P+RG  + +D  MVELTLD  +N+TGDIWHICVEDL
Sbjct: 115  EVENGINFAIFSQHATAIVLCLFLPQRGKKDRMDDLMVELTLDADVNRTGDIWHICVEDL 174

Query: 462  PRDGVLYGYRIDGPRGWKQGHRFDSSIILLDPYAKLVEGRRIFGDVSHKMSKFLGTYDFT 283
            PR  VLYGY IDGPR W QGHRFD+S +L+DPYAKLVEGRR FGD S+K SKFLGTYDF 
Sbjct: 175  PRSNVLYGYCIDGPRDWHQGHRFDNSTVLIDPYAKLVEGRRFFGDASNKWSKFLGTYDFD 234

Query: 282  SLPFDWGNNYKQPNIPEKDLVIYEMNVRAFTADNSSGLDQNIRGSYLGVIEKIPHLLELG 103
            SLPFDWG+NYK P+IPEKDLVIYEMNVRAFTAD SSGLD N+RGSYLGVIEKIPHLL+LG
Sbjct: 235  SLPFDWGDNYKVPSIPEKDLVIYEMNVRAFTADKSSGLDPNVRGSYLGVIEKIPHLLDLG 294

Query: 102  INAVELLPVFEFDELEFQRYPNPRDHMINTWGYS 1
            INAVELLPVFEFDE EFQR PNPRDHMINTWGYS
Sbjct: 295  INAVELLPVFEFDEFEFQRRPNPRDHMINTWGYS 328


>emb|CBI19205.3| unnamed protein product [Vitis vinifera]
          Length = 775

 Score =  428 bits (1100), Expect = e-117
 Identities = 213/322 (66%), Positives = 249/322 (77%)
 Frame = -2

Query: 966 MLHLNSHFCDSKTKLAPLSSRNPTRCASIEGFRLFTLRLRSSKKSVFGSNEQTFISKVRE 787
           M+H      ++K  L    S   +  +S    R+  + L  SKK V GSN +  +SK   
Sbjct: 1   MIHPLVGESNAKFPLFSALSNRDSGASSFGTSRIIGMGLLLSKK-VSGSNMKRTVSKTW- 58

Query: 786 SDRINRKFTSVYNRRAQESVIEEEAPEMSEISGPFKVSPGQAFPLGASEVENGINFSLFS 607
            D +  + T+ Y R AQ+ V+EEEAP+++E S   KV PGQAFPLG SEVENGINF++FS
Sbjct: 59  -DCLRPRTTNAYGRHAQDRVLEEEAPQIAETSPSLKVLPGQAFPLGVSEVENGINFAIFS 117

Query: 606 RHASAVTLCLTIPERGNHEELDSGMVELTLDPSINKTGDIWHICVEDLPRDGVLYGYRID 427
           +HA+A+ LCL +P+RG  + +D  MVELTLD  +N+TGDIWHICVEDLPR  VLYGY ID
Sbjct: 118 QHATAIVLCLFLPQRGKKDRMDDLMVELTLDADVNRTGDIWHICVEDLPRSNVLYGYCID 177

Query: 426 GPRGWKQGHRFDSSIILLDPYAKLVEGRRIFGDVSHKMSKFLGTYDFTSLPFDWGNNYKQ 247
           GPR W QGHRFD+S +L+DPYAKLVEGRR FGD S+K SKFLGTYDF SLPFDWG+NYK 
Sbjct: 178 GPRDWHQGHRFDNSTVLIDPYAKLVEGRRFFGDASNKWSKFLGTYDFDSLPFDWGDNYKV 237

Query: 246 PNIPEKDLVIYEMNVRAFTADNSSGLDQNIRGSYLGVIEKIPHLLELGINAVELLPVFEF 67
           P+IPEKDLVIYEMNVRAFTAD SSGLD N+RGSYLGVIEKIPHLL+LGINAVELLPVFEF
Sbjct: 238 PSIPEKDLVIYEMNVRAFTADKSSGLDPNVRGSYLGVIEKIPHLLDLGINAVELLPVFEF 297

Query: 66  DELEFQRYPNPRDHMINTWGYS 1
           DE EFQR PNPRDHMINTWGYS
Sbjct: 298 DEFEFQRRPNPRDHMINTWGYS 319


>ref|XP_007018697.1| Isoamylase 3 [Theobroma cacao] gi|508724025|gb|EOY15922.1|
           Isoamylase 3 [Theobroma cacao]
          Length = 778

 Score =  424 bits (1091), Expect = e-116
 Identities = 210/324 (64%), Positives = 248/324 (76%), Gaps = 2/324 (0%)
 Frame = -2

Query: 966 MLHLNSHFCDSKTKLAPLSSRNPTRCA--SIEGFRLFTLRLRSSKKSVFGSNEQTFISKV 793
           MLH +     S TK A  +S   T  +  S     L  + L+ S+ +   S  Q   SK 
Sbjct: 1   MLHSSFLSESSSTKFALFTSVVSTSASPSSTGNSSLLQMGLKWSRNASSSSGGQGRSSKA 60

Query: 792 RESDRINRKFTSVYNRRAQESVIEEEAPEMSEISGPFKVSPGQAFPLGASEVENGINFSL 613
           R   R+ R+ T  Y R+A+E V+EEEAPEMSE    FK+ PGQAFPLG SEV+NGINF++
Sbjct: 61  R--GRVKRRTTKAYGRQARERVLEEEAPEMSETIPSFKIFPGQAFPLGVSEVDNGINFAI 118

Query: 612 FSRHASAVTLCLTIPERGNHEELDSGMVELTLDPSINKTGDIWHICVEDLPRDGVLYGYR 433
           FS+HA+AVTLCL++P+RG  + L   M+E +LDP +NKTGDIWHIC+EDLPR  VLYGYR
Sbjct: 119 FSQHANAVTLCLSLPQRGELDRLAGSMIEFSLDPRVNKTGDIWHICIEDLPRSNVLYGYR 178

Query: 432 IDGPRGWKQGHRFDSSIILLDPYAKLVEGRRIFGDVSHKMSKFLGTYDFTSLPFDWGNNY 253
           +DGP+ W +GHRFDSS +L+DPYAKLVEGRR FGD  HK+SKFLGTYDF SLPFDWG+NY
Sbjct: 179 MDGPKDWDKGHRFDSSNVLIDPYAKLVEGRRHFGDPKHKLSKFLGTYDFDSLPFDWGDNY 238

Query: 252 KQPNIPEKDLVIYEMNVRAFTADNSSGLDQNIRGSYLGVIEKIPHLLELGINAVELLPVF 73
             PNIPEKDLV++EMNVRAFTAD SS LD N+RG+Y G+IEKIPHLLELGINAVELLPVF
Sbjct: 239 TLPNIPEKDLVMFEMNVRAFTADESSRLDPNLRGTYAGLIEKIPHLLELGINAVELLPVF 298

Query: 72  EFDELEFQRYPNPRDHMINTWGYS 1
           EFDE EFQR PNPRDHMINTWGYS
Sbjct: 299 EFDEFEFQRRPNPRDHMINTWGYS 322


>ref|XP_006472546.1| PREDICTED: isoamylase 3, chloroplastic-like [Citrus sinensis]
          Length = 782

 Score =  424 bits (1090), Expect = e-116
 Identities = 212/328 (64%), Positives = 248/328 (75%), Gaps = 3/328 (0%)
 Frame = -2

Query: 975 GTQMLHLNSHFCDSKTKL---APLSSRNPTRCASIEGFRLFTLRLRSSKKSVFGSNEQTF 805
           GT M+H       + TKL   AP  S    R  S        + LR SKK+  G+  + F
Sbjct: 6   GTMMIHQPLPALSNSTKLLLFAPTFSHKLCRSFSAAS-PAHEMGLRLSKKASSGNTPRHF 64

Query: 804 ISKVRESDRINRKFTSVYNRRAQESVIEEEAPEMSEISGPFKVSPGQAFPLGASEVENGI 625
               + S  +     + YN+R QE V+EEE+P +SE + P K S GQAFP+G SEVENGI
Sbjct: 65  N---KTSSSVKHTTPNAYNQRTQERVLEEESPRVSETTLPLKTSQGQAFPMGVSEVENGI 121

Query: 624 NFSLFSRHASAVTLCLTIPERGNHEELDSGMVELTLDPSINKTGDIWHICVEDLPRDGVL 445
           NF++FS+HA+AVTLCL++P+R   +  D GM+EL LDP +NKTGDIWHIC+EDLPR  VL
Sbjct: 122 NFAIFSQHATAVTLCLSLPKREKLDWQDGGMIELPLDPRVNKTGDIWHICIEDLPRSEVL 181

Query: 444 YGYRIDGPRGWKQGHRFDSSIILLDPYAKLVEGRRIFGDVSHKMSKFLGTYDFTSLPFDW 265
           YGYR+DGPR W QGHRFDSSI+L+DPYAKLVEGRR FGD S K+SKFLGTY+F SLPFDW
Sbjct: 182 YGYRVDGPRDWHQGHRFDSSIVLIDPYAKLVEGRRHFGDASAKLSKFLGTYEFESLPFDW 241

Query: 264 GNNYKQPNIPEKDLVIYEMNVRAFTADNSSGLDQNIRGSYLGVIEKIPHLLELGINAVEL 85
           G+NYK PNIPEKDLVIYEMNVRAFT D SSGLD  IRGSYLG+I+KIPHLLELGINAVEL
Sbjct: 242 GDNYKLPNIPEKDLVIYEMNVRAFTVDESSGLDPEIRGSYLGLIQKIPHLLELGINAVEL 301

Query: 84  LPVFEFDELEFQRYPNPRDHMINTWGYS 1
           LPVFEFDE+EFQR  NPRDHM+NTWGYS
Sbjct: 302 LPVFEFDEMEFQRRRNPRDHMVNTWGYS 329


>ref|XP_006433917.1| hypothetical protein CICLE_v10000347mg [Citrus clementina]
           gi|557536039|gb|ESR47157.1| hypothetical protein
           CICLE_v10000347mg [Citrus clementina]
          Length = 782

 Score =  422 bits (1086), Expect = e-116
 Identities = 213/328 (64%), Positives = 245/328 (74%), Gaps = 3/328 (0%)
 Frame = -2

Query: 975 GTQMLHLNSHFCDSKTKL---APLSSRNPTRCASIEGFRLFTLRLRSSKKSVFGSNEQTF 805
           GT M+H       + TKL   AP  S    R  S        + LR SKK   G+    F
Sbjct: 6   GTMMIHQPLPALSNSTKLLLFAPTLSHKLYRSFSAAS-PAHEMGLRLSKKVSRGNTPSNF 64

Query: 804 ISKVRESDRINRKFTSVYNRRAQESVIEEEAPEMSEISGPFKVSPGQAFPLGASEVENGI 625
               + S  +     S YN+  QE V+EEE+P +SE + P K S GQAFP+G SEVENGI
Sbjct: 65  N---KTSSSVKHTTPSAYNQPTQERVLEEESPRVSETTLPLKTSQGQAFPIGVSEVENGI 121

Query: 624 NFSLFSRHASAVTLCLTIPERGNHEELDSGMVELTLDPSINKTGDIWHICVEDLPRDGVL 445
           NF++FS+HA+AVTLCL++P+R   +  D GM+EL LDP +NKTGDIWHIC+EDLPR  VL
Sbjct: 122 NFAIFSQHATAVTLCLSLPKREKLDWQDGGMIELPLDPCVNKTGDIWHICIEDLPRSEVL 181

Query: 444 YGYRIDGPRGWKQGHRFDSSIILLDPYAKLVEGRRIFGDVSHKMSKFLGTYDFTSLPFDW 265
           YGYR+DGPR W QGHRFDSSI+L+DPYAKLVEGRR FGD S K+SKFLGTYDF SLPFDW
Sbjct: 182 YGYRVDGPRDWHQGHRFDSSIVLIDPYAKLVEGRRHFGDASAKLSKFLGTYDFESLPFDW 241

Query: 264 GNNYKQPNIPEKDLVIYEMNVRAFTADNSSGLDQNIRGSYLGVIEKIPHLLELGINAVEL 85
           G+NYK PNIPEKDLVIYEMNVRAFT D SSGLD  IRGSYLG+I+KIPHLLELGINAVEL
Sbjct: 242 GDNYKLPNIPEKDLVIYEMNVRAFTGDESSGLDPEIRGSYLGLIQKIPHLLELGINAVEL 301

Query: 84  LPVFEFDELEFQRYPNPRDHMINTWGYS 1
           LPVFEFDE+EFQR  NPRDHM+NTWGYS
Sbjct: 302 LPVFEFDEMEFQRRRNPRDHMVNTWGYS 329


>ref|XP_006433916.1| hypothetical protein CICLE_v10000347mg [Citrus clementina]
           gi|557536038|gb|ESR47156.1| hypothetical protein
           CICLE_v10000347mg [Citrus clementina]
          Length = 704

 Score =  422 bits (1086), Expect = e-116
 Identities = 213/328 (64%), Positives = 245/328 (74%), Gaps = 3/328 (0%)
 Frame = -2

Query: 975 GTQMLHLNSHFCDSKTKL---APLSSRNPTRCASIEGFRLFTLRLRSSKKSVFGSNEQTF 805
           GT M+H       + TKL   AP  S    R  S        + LR SKK   G+    F
Sbjct: 6   GTMMIHQPLPALSNSTKLLLFAPTLSHKLYRSFSAAS-PAHEMGLRLSKKVSRGNTPSNF 64

Query: 804 ISKVRESDRINRKFTSVYNRRAQESVIEEEAPEMSEISGPFKVSPGQAFPLGASEVENGI 625
               + S  +     S YN+  QE V+EEE+P +SE + P K S GQAFP+G SEVENGI
Sbjct: 65  N---KTSSSVKHTTPSAYNQPTQERVLEEESPRVSETTLPLKTSQGQAFPIGVSEVENGI 121

Query: 624 NFSLFSRHASAVTLCLTIPERGNHEELDSGMVELTLDPSINKTGDIWHICVEDLPRDGVL 445
           NF++FS+HA+AVTLCL++P+R   +  D GM+EL LDP +NKTGDIWHIC+EDLPR  VL
Sbjct: 122 NFAIFSQHATAVTLCLSLPKREKLDWQDGGMIELPLDPCVNKTGDIWHICIEDLPRSEVL 181

Query: 444 YGYRIDGPRGWKQGHRFDSSIILLDPYAKLVEGRRIFGDVSHKMSKFLGTYDFTSLPFDW 265
           YGYR+DGPR W QGHRFDSSI+L+DPYAKLVEGRR FGD S K+SKFLGTYDF SLPFDW
Sbjct: 182 YGYRVDGPRDWHQGHRFDSSIVLIDPYAKLVEGRRHFGDASAKLSKFLGTYDFESLPFDW 241

Query: 264 GNNYKQPNIPEKDLVIYEMNVRAFTADNSSGLDQNIRGSYLGVIEKIPHLLELGINAVEL 85
           G+NYK PNIPEKDLVIYEMNVRAFT D SSGLD  IRGSYLG+I+KIPHLLELGINAVEL
Sbjct: 242 GDNYKLPNIPEKDLVIYEMNVRAFTGDESSGLDPEIRGSYLGLIQKIPHLLELGINAVEL 301

Query: 84  LPVFEFDELEFQRYPNPRDHMINTWGYS 1
           LPVFEFDE+EFQR  NPRDHM+NTWGYS
Sbjct: 302 LPVFEFDEMEFQRRRNPRDHMVNTWGYS 329


>ref|XP_003527894.1| PREDICTED: isoamylase 3, chloroplastic-like [Glycine max]
          Length = 772

 Score =  422 bits (1085), Expect = e-115
 Identities = 210/317 (66%), Positives = 245/317 (77%), Gaps = 7/317 (2%)
 Frame = -2

Query: 930 TKLAPLSS--RNPTRCA-----SIEGFRLFTLRLRSSKKSVFGSNEQTFISKVRESDRIN 772
           T  AP+ S   N  RC+     S+   R  +LR          +NE    S+    DR  
Sbjct: 6   TLFAPIFSGGNNAGRCSFAGNSSVRDVR--SLRFMKQASGRQSNNEHGVFSETM--DRAK 61

Query: 771 RKFTSVYNRRAQESVIEEEAPEMSEISGPFKVSPGQAFPLGASEVENGINFSLFSRHASA 592
            + T+ Y  RAQE V+EEE+ ++ E    +K  PGQ+FPLG SEV++GINF++FS++A+A
Sbjct: 62  HRATTAYGGRAQEGVLEEESSKVIESRPSWKAFPGQSFPLGVSEVDSGINFAIFSQNATA 121

Query: 591 VTLCLTIPERGNHEELDSGMVELTLDPSINKTGDIWHICVEDLPRDGVLYGYRIDGPRGW 412
           VTLCL +PERG+ + LD GM+E+ LDP +NKTGDIWHIC+EDLPR  VLYGYRIDGPR W
Sbjct: 122 VTLCLVLPERGSIDALDGGMIEMVLDPDLNKTGDIWHICIEDLPRSNVLYGYRIDGPRDW 181

Query: 411 KQGHRFDSSIILLDPYAKLVEGRRIFGDVSHKMSKFLGTYDFTSLPFDWGNNYKQPNIPE 232
            +GHRFDSSI+L+DPYAKLVEGRR FGD+S K+SKFLGTYDF SLPFDWG NYK PNI E
Sbjct: 182 GKGHRFDSSIVLVDPYAKLVEGRRYFGDISMKLSKFLGTYDFDSLPFDWGENYKLPNISE 241

Query: 231 KDLVIYEMNVRAFTADNSSGLDQNIRGSYLGVIEKIPHLLELGINAVELLPVFEFDELEF 52
           KDLVIYEMNVRAFT+D SSGLD NIRGSYLG+IEKIPHLLELGINAVELLPVFEFDELEF
Sbjct: 242 KDLVIYEMNVRAFTSDESSGLDSNIRGSYLGMIEKIPHLLELGINAVELLPVFEFDELEF 301

Query: 51  QRYPNPRDHMINTWGYS 1
           QR PNPRDHMINTWGYS
Sbjct: 302 QRRPNPRDHMINTWGYS 318


>ref|XP_003522781.1| PREDICTED: isoamylase 3, chloroplastic-like isoform X1 [Glycine
           max]
          Length = 783

 Score =  419 bits (1077), Expect = e-114
 Identities = 202/280 (72%), Positives = 230/280 (82%), Gaps = 1/280 (0%)
 Frame = -2

Query: 837 KSVFGSNEQTFISKVRES-DRINRKFTSVYNRRAQESVIEEEAPEMSEISGPFKVSPGQA 661
           K   GS     +    E+ DR   + T+VY  RAQE V+EEE  ++ E    +KV PGQA
Sbjct: 50  KQASGSKSNNELGVFSETMDRARHRTTTVYGGRAQEGVLEEETSKVIESRPSWKVFPGQA 109

Query: 660 FPLGASEVENGINFSLFSRHASAVTLCLTIPERGNHEELDSGMVELTLDPSINKTGDIWH 481
           FPLG SEV+N +NF++FS+HA+AVTLCL +PERG+ + LD GM+E+ LDP +NKTGDIWH
Sbjct: 110 FPLGVSEVDNCMNFAIFSQHATAVTLCLVLPERGSIDALDGGMIEMVLDPDLNKTGDIWH 169

Query: 480 ICVEDLPRDGVLYGYRIDGPRGWKQGHRFDSSIILLDPYAKLVEGRRIFGDVSHKMSKFL 301
           IC+EDLPR  VLYGYRIDGP    +GHRFDSSI+L+DPYAKLVEGRR FGD+S K+SKFL
Sbjct: 170 ICIEDLPRSNVLYGYRIDGPGDRGKGHRFDSSIVLVDPYAKLVEGRRYFGDISLKLSKFL 229

Query: 300 GTYDFTSLPFDWGNNYKQPNIPEKDLVIYEMNVRAFTADNSSGLDQNIRGSYLGVIEKIP 121
           GTYDF SLPFDWG NYK PNI EKDLVIYEMNVRAFT+D SSGLD NIRGSYLGVIEKIP
Sbjct: 230 GTYDFDSLPFDWGENYKLPNISEKDLVIYEMNVRAFTSDESSGLDSNIRGSYLGVIEKIP 289

Query: 120 HLLELGINAVELLPVFEFDELEFQRYPNPRDHMINTWGYS 1
           HLLELGINAVELLPVFEFDELEFQR+PNPRDHMINTWGYS
Sbjct: 290 HLLELGINAVELLPVFEFDELEFQRFPNPRDHMINTWGYS 329


>ref|XP_006578313.1| PREDICTED: isoamylase 3, chloroplastic-like isoform X3 [Glycine
           max]
          Length = 716

 Score =  418 bits (1075), Expect = e-114
 Identities = 198/261 (75%), Positives = 224/261 (85%)
 Frame = -2

Query: 783 DRINRKFTSVYNRRAQESVIEEEAPEMSEISGPFKVSPGQAFPLGASEVENGINFSLFSR 604
           DR   + T+VY  RAQE V+EEE  ++ E    +KV PGQAFPLG SEV+N +NF++FS+
Sbjct: 2   DRARHRTTTVYGGRAQEGVLEEETSKVIESRPSWKVFPGQAFPLGVSEVDNCMNFAIFSQ 61

Query: 603 HASAVTLCLTIPERGNHEELDSGMVELTLDPSINKTGDIWHICVEDLPRDGVLYGYRIDG 424
           HA+AVTLCL +PERG+ + LD GM+E+ LDP +NKTGDIWHIC+EDLPR  VLYGYRIDG
Sbjct: 62  HATAVTLCLVLPERGSIDALDGGMIEMVLDPDLNKTGDIWHICIEDLPRSNVLYGYRIDG 121

Query: 423 PRGWKQGHRFDSSIILLDPYAKLVEGRRIFGDVSHKMSKFLGTYDFTSLPFDWGNNYKQP 244
           P    +GHRFDSSI+L+DPYAKLVEGRR FGD+S K+SKFLGTYDF SLPFDWG NYK P
Sbjct: 122 PGDRGKGHRFDSSIVLVDPYAKLVEGRRYFGDISLKLSKFLGTYDFDSLPFDWGENYKLP 181

Query: 243 NIPEKDLVIYEMNVRAFTADNSSGLDQNIRGSYLGVIEKIPHLLELGINAVELLPVFEFD 64
           NI EKDLVIYEMNVRAFT+D SSGLD NIRGSYLGVIEKIPHLLELGINAVELLPVFEFD
Sbjct: 182 NISEKDLVIYEMNVRAFTSDESSGLDSNIRGSYLGVIEKIPHLLELGINAVELLPVFEFD 241

Query: 63  ELEFQRYPNPRDHMINTWGYS 1
           ELEFQR+PNPRDHMINTWGYS
Sbjct: 242 ELEFQRFPNPRDHMINTWGYS 262


>ref|XP_007136280.1| hypothetical protein PHAVU_009G033200g [Phaseolus vulgaris]
           gi|139867062|dbj|BAF52943.1| isoamylase-type
           starch-debranching enzyme 3 [Phaseolus vulgaris]
           gi|561009367|gb|ESW08274.1| hypothetical protein
           PHAVU_009G033200g [Phaseolus vulgaris]
          Length = 783

 Score =  417 bits (1071), Expect = e-114
 Identities = 199/286 (69%), Positives = 232/286 (81%)
 Frame = -2

Query: 858 LRLRSSKKSVFGSNEQTFISKVRESDRINRKFTSVYNRRAQESVIEEEAPEMSEISGPFK 679
           +RL         +NE+   S++   DR   K    Y RRAQE V++EE+ ++ E     K
Sbjct: 46  VRLMKQASGSITNNERGVFSEIM--DRHKLKTPDAYGRRAQEGVLQEESSKVMESRPSGK 103

Query: 678 VSPGQAFPLGASEVENGINFSLFSRHASAVTLCLTIPERGNHEELDSGMVELTLDPSINK 499
           + PGQAFPLG SEV++GINFS+FS+HA+AVTLCL +PERG+ + ++  M+E+ LDP +NK
Sbjct: 104 IFPGQAFPLGVSEVDSGINFSIFSQHATAVTLCLVLPERGSIDTMNGDMIEVVLDPDLNK 163

Query: 498 TGDIWHICVEDLPRDGVLYGYRIDGPRGWKQGHRFDSSIILLDPYAKLVEGRRIFGDVSH 319
           TGDIWHIC+EDLPR  VLYGYRIDGP+ W +GHRFD+SI+L+DPYAKLVEGRR FGD+S 
Sbjct: 164 TGDIWHICIEDLPRSNVLYGYRIDGPQDWGKGHRFDNSIVLVDPYAKLVEGRRYFGDIST 223

Query: 318 KMSKFLGTYDFTSLPFDWGNNYKQPNIPEKDLVIYEMNVRAFTADNSSGLDQNIRGSYLG 139
           K+S+FLGTYDF SLPFDWG NYK PNI EKDLVIYEMNVRAFT D SSGLD NIRGSYLG
Sbjct: 224 KLSRFLGTYDFDSLPFDWGENYKLPNISEKDLVIYEMNVRAFTFDESSGLDSNIRGSYLG 283

Query: 138 VIEKIPHLLELGINAVELLPVFEFDELEFQRYPNPRDHMINTWGYS 1
           VIEKIPHLLELGINAVELLPVFEFDELEFQR PNPRDHMINTWGYS
Sbjct: 284 VIEKIPHLLELGINAVELLPVFEFDELEFQRRPNPRDHMINTWGYS 329


>ref|XP_002513977.1| isoamylase, putative [Ricinus communis] gi|223547063|gb|EEF48560.1|
           isoamylase, putative [Ricinus communis]
          Length = 783

 Score =  416 bits (1068), Expect = e-113
 Identities = 194/261 (74%), Positives = 220/261 (84%), Gaps = 1/261 (0%)
 Frame = -2

Query: 780 RINRKFTSVYNRRAQESVIEEE-APEMSEISGPFKVSPGQAFPLGASEVENGINFSLFSR 604
           R   +  +VY R AQE V++EE   +MSE++  F + PGQAFPLG SEV+NGINF+LFS+
Sbjct: 66  RAKHRTPNVYARGAQERVLQEEKVSQMSEMTPSFNLYPGQAFPLGVSEVDNGINFALFSQ 125

Query: 603 HASAVTLCLTIPERGNHEELDSGMVELTLDPSINKTGDIWHICVEDLPRDGVLYGYRIDG 424
           HA++VTLCL +P+RG  +  D GM+EL LDP +NKTGDIWHICVEDLPR  VLYGYR+DG
Sbjct: 126 HATSVTLCLLLPQRGGSDSTDGGMIELDLDPRMNKTGDIWHICVEDLPRSSVLYGYRVDG 185

Query: 423 PRGWKQGHRFDSSIILLDPYAKLVEGRRIFGDVSHKMSKFLGTYDFTSLPFDWGNNYKQP 244
           P  W QGHRFD SI+LLDPYAKL+EGRR FGD +HK+SKFLGTYDF SLPFDWG NYK P
Sbjct: 186 PHNWDQGHRFDRSIVLLDPYAKLIEGRRYFGDATHKLSKFLGTYDFDSLPFDWGENYKLP 245

Query: 243 NIPEKDLVIYEMNVRAFTADNSSGLDQNIRGSYLGVIEKIPHLLELGINAVELLPVFEFD 64
           NIPEKDLVIYEMN+RAFTAD SSGL+  IRGSYLGVIEKIPHLLELG+NAVELLPVFEFD
Sbjct: 246 NIPEKDLVIYEMNIRAFTADKSSGLEPKIRGSYLGVIEKIPHLLELGVNAVELLPVFEFD 305

Query: 63  ELEFQRYPNPRDHMINTWGYS 1
           E E QR PNPRDHMINTWGYS
Sbjct: 306 EFELQRRPNPRDHMINTWGYS 326


>ref|XP_002301076.2| isoamylase family protein [Populus trichocarpa]
            gi|550344682|gb|EEE80349.2| isoamylase family protein
            [Populus trichocarpa]
          Length = 819

 Score =  410 bits (1053), Expect = e-112
 Identities = 216/340 (63%), Positives = 245/340 (72%), Gaps = 26/340 (7%)
 Frame = -2

Query: 942  CDSKTKLAPLSS----RNPTRCASIEGFRLFTLRLRSSKKSVFG-SNEQTFISKVRESDR 778
            CDSK   + L S     N     SI   R+  + L+ SK+S  G S+ + F SK      
Sbjct: 10   CDSKIADSILLSVSHFSNNNNNNSIGSSRVLDVGLKFSKQSSGGGSSGRGFSSKAGAKTT 69

Query: 777  INRKFTSVYNRRAQESVIE-EEAPEMSEISGPFKVSPGQAFPLGASEVENGINFSLFSRH 601
                  +VY RRAQESV+E EEAP+       FK  PGQAFP G S+VENGINF++FS+H
Sbjct: 70   TT---PNVYGRRAQESVLEQEEAPQKLG----FKTFPGQAFPFGVSQVENGINFAIFSQH 122

Query: 600  ASAVTLCLTIPERGNHEELDSGMVELTLDPSINKTGDIWHICVEDLPRDGVLYGYRIDGP 421
            A+AVTLCL++P RG  E  D GM+E+ LDP +NKTGDIWHIC+EDLPRD VLYGYRIDGP
Sbjct: 123  ATAVTLCLSLPHRGKSERTDGGMIEVALDPKVNKTGDIWHICIEDLPRDDVLYGYRIDGP 182

Query: 420  RGWKQGHRFDSSIILLDPYAKLVEGRRIFGDVSHKMSKFLGTYDFTSLPFDWGNNYKQPN 241
            R W+QGHRFDSSI+L+DPYAKLVE RR FGD S K+SKF GTYDF SLPFDWG++YK PN
Sbjct: 183  RDWRQGHRFDSSIMLIDPYAKLVESRRFFGDASRKLSKFYGTYDFDSLPFDWGDDYKPPN 242

Query: 240  IPEKDLVIYEMNVRAFTADNSSGLDQNIRGSYLGVIE--------------------KIP 121
            IPEKDLVIYEMNVRAFT D SSGLD +IRGSYLGVIE                    KIP
Sbjct: 243  IPEKDLVIYEMNVRAFTVDKSSGLDPSIRGSYLGVIEKWYLIACTYLVLLTISFHSIKIP 302

Query: 120  HLLELGINAVELLPVFEFDELEFQRYPNPRDHMINTWGYS 1
            HLLELG+NAVELLPVFEFDE EFQR PNPRDHMINTWGYS
Sbjct: 303  HLLELGVNAVELLPVFEFDEFEFQRRPNPRDHMINTWGYS 342


>ref|XP_004501686.1| PREDICTED: isoamylase 3, chloroplastic-like isoform X1 [Cicer
           arietinum]
          Length = 780

 Score =  407 bits (1046), Expect = e-111
 Identities = 198/308 (64%), Positives = 236/308 (76%), Gaps = 1/308 (0%)
 Frame = -2

Query: 921 APLSSRNPTRCASIEGFRLFTLRLRSSKKSVFGSNE-QTFISKVRESDRINRKFTSVYNR 745
           A  ++  PT  A     R   L+  +       +N  +  +S+ R+  ++    T+ Y R
Sbjct: 20  AAANTPKPTSLAGNLSTRHIWLKFTNQATGSTSNNSCRGIVSETRDGVKLRTTNTNAYGR 79

Query: 744 RAQESVIEEEAPEMSEISGPFKVSPGQAFPLGASEVENGINFSLFSRHASAVTLCLTIPE 565
            AQE VI++EA +  E    +++SPGQ FPLG S+V+NG+NF++FS+HA+AVTLCL  PE
Sbjct: 80  -AQEEVIKDEAVKGMENKPSWEISPGQTFPLGVSQVDNGVNFAIFSQHATAVTLCLVFPE 138

Query: 564 RGNHEELDSGMVELTLDPSINKTGDIWHICVEDLPRDGVLYGYRIDGPRGWKQGHRFDSS 385
           RG    LD G++EL LDP +NKTGD+WHIC+EDLPR  VLYGYRIDGP+ W +GHRFD S
Sbjct: 139 RGPIGTLDGGIIELALDPHLNKTGDVWHICIEDLPRSNVLYGYRIDGPQDWGKGHRFDRS 198

Query: 384 IILLDPYAKLVEGRRIFGDVSHKMSKFLGTYDFTSLPFDWGNNYKQPNIPEKDLVIYEMN 205
           I+L+DPYAKL+EGRR FGD+S K+SKFLGTYDF SLPFDWG NYK PNI EKDLVIYEMN
Sbjct: 199 IVLVDPYAKLIEGRRHFGDISMKLSKFLGTYDFDSLPFDWGENYKLPNIAEKDLVIYEMN 258

Query: 204 VRAFTADNSSGLDQNIRGSYLGVIEKIPHLLELGINAVELLPVFEFDELEFQRYPNPRDH 25
           VRAFT D SSGLD NIRGSYLGVIEKIPHLLELGINAVELLP+FEFDELE QR PNPRDH
Sbjct: 259 VRAFTNDESSGLDNNIRGSYLGVIEKIPHLLELGINAVELLPIFEFDELELQRRPNPRDH 318

Query: 24  MINTWGYS 1
           MINTWGYS
Sbjct: 319 MINTWGYS 326


>ref|XP_006578312.1| PREDICTED: isoamylase 3, chloroplastic-like isoform X2 [Glycine
           max]
          Length = 760

 Score =  399 bits (1025), Expect = e-108
 Identities = 188/241 (78%), Positives = 211/241 (87%)
 Frame = -2

Query: 723 EEEAPEMSEISGPFKVSPGQAFPLGASEVENGINFSLFSRHASAVTLCLTIPERGNHEEL 544
           EEE  ++ E    +KV PGQAFPLG SEV+N +NF++FS+HA+AVTLCL +PERG+ + L
Sbjct: 66  EEETSKVIESRPSWKVFPGQAFPLGVSEVDNCMNFAIFSQHATAVTLCLVLPERGSIDAL 125

Query: 543 DSGMVELTLDPSINKTGDIWHICVEDLPRDGVLYGYRIDGPRGWKQGHRFDSSIILLDPY 364
           D GM+E+ LDP +NKTGDIWHIC+EDLPR  VLYGYRIDGP    +GHRFDSSI+L+DPY
Sbjct: 126 DGGMIEMVLDPDLNKTGDIWHICIEDLPRSNVLYGYRIDGPGDRGKGHRFDSSIVLVDPY 185

Query: 363 AKLVEGRRIFGDVSHKMSKFLGTYDFTSLPFDWGNNYKQPNIPEKDLVIYEMNVRAFTAD 184
           AKLVEGRR FGD+S K+SKFLGTYDF SLPFDWG NYK PNI EKDLVIYEMNVRAFT+D
Sbjct: 186 AKLVEGRRYFGDISLKLSKFLGTYDFDSLPFDWGENYKLPNISEKDLVIYEMNVRAFTSD 245

Query: 183 NSSGLDQNIRGSYLGVIEKIPHLLELGINAVELLPVFEFDELEFQRYPNPRDHMINTWGY 4
            SSGLD NIRGSYLGVIEKIPHLLELGINAVELLPVFEFDELEFQR+PNPRDHMINTWGY
Sbjct: 246 ESSGLDSNIRGSYLGVIEKIPHLLELGINAVELLPVFEFDELEFQRFPNPRDHMINTWGY 305

Query: 3   S 1
           S
Sbjct: 306 S 306


>ref|XP_004299680.1| PREDICTED: isoamylase 3, chloroplastic-like [Fragaria vesca subsp.
            vesca]
          Length = 797

 Score =  398 bits (1023), Expect = e-108
 Identities = 200/333 (60%), Positives = 239/333 (71%), Gaps = 6/333 (1%)
 Frame = -2

Query: 981  QHGTQMLHLNSHFCDSKTKLAPL-SSRNPTRCAS-----IEGFRLFTLRLRSSKKSVFGS 820
            +    +L    H  DS     PL  S +P    +     +    +    L+ SK++  GS
Sbjct: 14   ERSKMLLRTTPHLYDSNVSRFPLFGSASPISTQAGVSSCVVASPVLDTGLKLSKQAT-GS 72

Query: 819  NEQTFISKVRESDRINRKFTSVYNRRAQESVIEEEAPEMSEISGPFKVSPGQAFPLGASE 640
            ++Q  + K R  D +     + Y + A  SV+EEEA ++ E S P++ SPGQA PLG SE
Sbjct: 73   SQQGLVRKTR--DHVKHSTANAYGQGAPGSVLEEEA-QLPETSQPWETSPGQASPLGVSE 129

Query: 639  VENGINFSLFSRHASAVTLCLTIPERGNHEELDSGMVELTLDPSINKTGDIWHICVEDLP 460
            V+ G+NF++FS+HA+AVTLC+ + +RG     D GM EL LDP+ NKTGDIWHIC++D P
Sbjct: 130  VDRGVNFAIFSQHATAVTLCIKVADRGQFGGPDGGMTELALDPNENKTGDIWHICIKDFP 189

Query: 459  RDGVLYGYRIDGPRGWKQGHRFDSSIILLDPYAKLVEGRRIFGDVSHKMSKFLGTYDFTS 280
            R  VLYGYRIDGP GW QGHRFDSSI+L+DPYAKLVEGRR FGD   K+S FLGTYDF S
Sbjct: 190  RSNVLYGYRIDGPHGWDQGHRFDSSIVLIDPYAKLVEGRRCFGDSREKLSGFLGTYDFDS 249

Query: 279  LPFDWGNNYKQPNIPEKDLVIYEMNVRAFTADNSSGLDQNIRGSYLGVIEKIPHLLELGI 100
             PFDWG+NYK PNIPE DLVIYEMNVRAFTAD SSGLD N+RGSY G+I+KIPHLLELGI
Sbjct: 250  SPFDWGDNYKLPNIPENDLVIYEMNVRAFTADESSGLDPNVRGSYRGLIDKIPHLLELGI 309

Query: 99   NAVELLPVFEFDELEFQRYPNPRDHMINTWGYS 1
            NAVELLPVFEFDE EFQR  NPRDHMINTWGYS
Sbjct: 310  NAVELLPVFEFDEFEFQRRSNPRDHMINTWGYS 342


>ref|XP_004501687.1| PREDICTED: isoamylase 3, chloroplastic-like isoform X2 [Cicer
           arietinum]
          Length = 756

 Score =  396 bits (1017), Expect = e-108
 Identities = 186/258 (72%), Positives = 213/258 (82%)
 Frame = -2

Query: 774 NRKFTSVYNRRAQESVIEEEAPEMSEISGPFKVSPGQAFPLGASEVENGINFSLFSRHAS 595
           N+   S  N   +  V E+EA +  E    +++SPGQ FPLG S+V+NG+NF++FS+HA+
Sbjct: 45  NQATGSTSNNSCRGIVSEDEAVKGMENKPSWEISPGQTFPLGVSQVDNGVNFAIFSQHAT 104

Query: 594 AVTLCLTIPERGNHEELDSGMVELTLDPSINKTGDIWHICVEDLPRDGVLYGYRIDGPRG 415
           AVTLCL  PERG    LD G++EL LDP +NKTGD+WHIC+EDLPR  VLYGYRIDGP+ 
Sbjct: 105 AVTLCLVFPERGPIGTLDGGIIELALDPHLNKTGDVWHICIEDLPRSNVLYGYRIDGPQD 164

Query: 414 WKQGHRFDSSIILLDPYAKLVEGRRIFGDVSHKMSKFLGTYDFTSLPFDWGNNYKQPNIP 235
           W +GHRFD SI+L+DPYAKL+EGRR FGD+S K+SKFLGTYDF SLPFDWG NYK PNI 
Sbjct: 165 WGKGHRFDRSIVLVDPYAKLIEGRRHFGDISMKLSKFLGTYDFDSLPFDWGENYKLPNIA 224

Query: 234 EKDLVIYEMNVRAFTADNSSGLDQNIRGSYLGVIEKIPHLLELGINAVELLPVFEFDELE 55
           EKDLVIYEMNVRAFT D SSGLD NIRGSYLGVIEKIPHLLELGINAVELLP+FEFDELE
Sbjct: 225 EKDLVIYEMNVRAFTNDESSGLDNNIRGSYLGVIEKIPHLLELGINAVELLPIFEFDELE 284

Query: 54  FQRYPNPRDHMINTWGYS 1
            QR PNPRDHMINTWGYS
Sbjct: 285 LQRRPNPRDHMINTWGYS 302


>gb|AAZ81837.1| isoamylase isoform 3 [Pisum sativum]
          Length = 736

 Score =  394 bits (1011), Expect = e-107
 Identities = 189/267 (70%), Positives = 219/267 (82%)
 Frame = -2

Query: 801 SKVRESDRINRKFTSVYNRRAQESVIEEEAPEMSEISGPFKVSPGQAFPLGASEVENGIN 622
           S+ RE  ++     + Y R AQE V ++EA ++ E    ++VSPGQAFPLG S+V+NGIN
Sbjct: 17  SETREGLKVRTTNANAYGR-AQEEVNKDEAFKVMETRPSWEVSPGQAFPLGVSQVDNGIN 75

Query: 621 FSLFSRHASAVTLCLTIPERGNHEELDSGMVELTLDPSINKTGDIWHICVEDLPRDGVLY 442
           F++FS+HA+AVTLCL +P+R + + LD G +EL LDP +NKTGDIWHI +EDL R  VLY
Sbjct: 76  FAIFSQHATAVTLCLVLPKRESIDTLDGGTIELALDPHLNKTGDIWHIRIEDLARSNVLY 135

Query: 441 GYRIDGPRGWKQGHRFDSSIILLDPYAKLVEGRRIFGDVSHKMSKFLGTYDFTSLPFDWG 262
           GYRIDG + W +GHRFD SI+L+DPYAKLVEGRR FGD+S K SKFLGTYDF SLPFDWG
Sbjct: 136 GYRIDGSQDWGKGHRFDRSIVLVDPYAKLVEGRRYFGDISKKFSKFLGTYDFDSLPFDWG 195

Query: 261 NNYKQPNIPEKDLVIYEMNVRAFTADNSSGLDQNIRGSYLGVIEKIPHLLELGINAVELL 82
            NYK PN+ EKDLVIYEMNVRAFT D SSGLD NIRGSYLGVIEKIPHLLELGINAVELL
Sbjct: 196 ENYKLPNVAEKDLVIYEMNVRAFTMDESSGLDNNIRGSYLGVIEKIPHLLELGINAVELL 255

Query: 81  PVFEFDELEFQRYPNPRDHMINTWGYS 1
           P+FEFDELE QR PNPRDHMINTWGYS
Sbjct: 256 PIFEFDELELQRRPNPRDHMINTWGYS 282


>ref|XP_004501688.1| PREDICTED: isoamylase 3, chloroplastic-like isoform X3 [Cicer
           arietinum]
          Length = 688

 Score =  389 bits (999), Expect = e-105
 Identities = 178/228 (78%), Positives = 201/228 (88%)
 Frame = -2

Query: 684 FKVSPGQAFPLGASEVENGINFSLFSRHASAVTLCLTIPERGNHEELDSGMVELTLDPSI 505
           +++SPGQ FPLG S+V+NG+NF++FS+HA+AVTLCL  PERG    LD G++EL LDP +
Sbjct: 7   WEISPGQTFPLGVSQVDNGVNFAIFSQHATAVTLCLVFPERGPIGTLDGGIIELALDPHL 66

Query: 504 NKTGDIWHICVEDLPRDGVLYGYRIDGPRGWKQGHRFDSSIILLDPYAKLVEGRRIFGDV 325
           NKTGD+WHIC+EDLPR  VLYGYRIDGP+ W +GHRFD SI+L+DPYAKL+EGRR FGD+
Sbjct: 67  NKTGDVWHICIEDLPRSNVLYGYRIDGPQDWGKGHRFDRSIVLVDPYAKLIEGRRHFGDI 126

Query: 324 SHKMSKFLGTYDFTSLPFDWGNNYKQPNIPEKDLVIYEMNVRAFTADNSSGLDQNIRGSY 145
           S K+SKFLGTYDF SLPFDWG NYK PNI EKDLVIYEMNVRAFT D SSGLD NIRGSY
Sbjct: 127 SMKLSKFLGTYDFDSLPFDWGENYKLPNIAEKDLVIYEMNVRAFTNDESSGLDNNIRGSY 186

Query: 144 LGVIEKIPHLLELGINAVELLPVFEFDELEFQRYPNPRDHMINTWGYS 1
           LGVIEKIPHLLELGINAVELLP+FEFDELE QR PNPRDHMINTWGYS
Sbjct: 187 LGVIEKIPHLLELGINAVELLPIFEFDELELQRRPNPRDHMINTWGYS 234


>ref|XP_002874494.1| ATISA3/ISA3 [Arabidopsis lyrata subsp. lyrata]
           gi|297320331|gb|EFH50753.1| ATISA3/ISA3 [Arabidopsis
           lyrata subsp. lyrata]
          Length = 766

 Score =  384 bits (986), Expect = e-104
 Identities = 191/304 (62%), Positives = 231/304 (75%)
 Frame = -2

Query: 912 SSRNPTRCASIEGFRLFTLRLRSSKKSVFGSNEQTFISKVRESDRINRKFTSVYNRRAQE 733
           S+ +P    +  GF +  + L  S+K +     + F  KV++     R    V  RRAQE
Sbjct: 18  SNFSPVLTNAFSGFTI-PMGLNFSRKVI---GARVFSRKVKD-----RSTLKVSCRRAQE 68

Query: 732 SVIEEEAPEMSEISGPFKVSPGQAFPLGASEVENGINFSLFSRHASAVTLCLTIPERGNH 553
            V+EEEA  M+E    FKVS G+  PLG S+V+NGINF+LFS++A++VTLCL++P+ G  
Sbjct: 69  RVVEEEASTMTETKQLFKVSTGEVSPLGVSQVDNGINFALFSQNATSVTLCLSLPQSGKD 128

Query: 552 EELDSGMVELTLDPSINKTGDIWHICVEDLPRDGVLYGYRIDGPRGWKQGHRFDSSIILL 373
           +  D GM+EL LDPS+NKTGD WHICVEDLP + VLYGYR+DGP  W+ GHRFD SI+LL
Sbjct: 129 DLSDDGMIELVLDPSVNKTGDTWHICVEDLPLNNVLYGYRVDGPGEWQHGHRFDHSILLL 188

Query: 372 DPYAKLVEGRRIFGDVSHKMSKFLGTYDFTSLPFDWGNNYKQPNIPEKDLVIYEMNVRAF 193
           DPYAKLV+GR  FGD S K ++F GTYDF S PF+WG++YK PNIPEKDLVIYEMNVRAF
Sbjct: 189 DPYAKLVKGRSSFGDSSQKFAQFYGTYDFESSPFNWGDDYKFPNIPEKDLVIYEMNVRAF 248

Query: 192 TADNSSGLDQNIRGSYLGVIEKIPHLLELGINAVELLPVFEFDELEFQRYPNPRDHMINT 13
           TAD SSG+D  IRGSYLG+IEKIPHL +LGINAVELLPVFEFDELE QR PNPRDHM+NT
Sbjct: 249 TADESSGMDPAIRGSYLGLIEKIPHLQDLGINAVELLPVFEFDELELQRRPNPRDHMVNT 308

Query: 12  WGYS 1
           WGYS
Sbjct: 309 WGYS 312


>ref|NP_001275220.1| isoamylase isoform 3 [Solanum tuberosum] gi|27728149|gb|AAN15319.1|
           isoamylase isoform 3 [Solanum tuberosum]
          Length = 766

 Score =  383 bits (983), Expect = e-104
 Identities = 178/242 (73%), Positives = 206/242 (85%)
 Frame = -2

Query: 726 IEEEAPEMSEISGPFKVSPGQAFPLGASEVENGINFSLFSRHASAVTLCLTIPERGNHEE 547
           ++EEAP+M +    FKVSPG A PLG SE E+GINF++FS+HASAVTLC+ +P+      
Sbjct: 74  LQEEAPQMLDTFPSFKVSPGLAHPLGVSETESGINFAIFSQHASAVTLCIILPK-----S 128

Query: 546 LDSGMVELTLDPSINKTGDIWHICVEDLPRDGVLYGYRIDGPRGWKQGHRFDSSIILLDP 367
           +  GM+EL LDP  N+TGDIWHIC+++LP+ GVLYGYRIDGPR W +GHRFD SIIL+DP
Sbjct: 129 VHDGMIELALDPQKNRTGDIWHICIKELPQGGVLYGYRIDGPRNWHEGHRFDDSIILVDP 188

Query: 366 YAKLVEGRRIFGDVSHKMSKFLGTYDFTSLPFDWGNNYKQPNIPEKDLVIYEMNVRAFTA 187
           YAKL+EGRR+FGD S+KM +F GTYDF SLPFDWG NYK PNIPEKDLVIYEMNVRAFTA
Sbjct: 189 YAKLIEGRRVFGDESNKMCRFFGTYDFNSLPFDWGENYKLPNIPEKDLVIYEMNVRAFTA 248

Query: 186 DNSSGLDQNIRGSYLGVIEKIPHLLELGINAVELLPVFEFDELEFQRYPNPRDHMINTWG 7
           D +S LDQ+ RGSYLG+IEKIPHLLELG+NAVELLPVFEFDELE QR PNPRDHMINTWG
Sbjct: 249 DETSSLDQDQRGSYLGLIEKIPHLLELGVNAVELLPVFEFDELELQRRPNPRDHMINTWG 308

Query: 6   YS 1
           YS
Sbjct: 309 YS 310


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