BLASTX nr result
ID: Aconitum23_contig00039480
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Aconitum23_contig00039480
(427 letters)
Database: ./nr
77,306,371 sequences; 28,104,191,420 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CCH50976.1| T4.15 [Malus x robusta] 55 4e-09
ref|WP_051127975.1| hypothetical protein [Bacillus isronensis] 52 7e-09
gb|EKB43273.1| hypothetical protein B857_03969 [Bacillus isronen... 52 8e-09
gb|AAT40504.2| Polyprotein, putative [Solanum demissum] 45 2e-08
gb|KHN04075.1| hypothetical protein glysoja_036672 [Glycine soja] 50 3e-08
ref|XP_010684010.1| PREDICTED: uncharacterized protein LOC104898... 48 3e-08
ref|WP_009187176.1| hypothetical protein [Cecembia lonarensis] g... 50 3e-08
gb|AEJ72569.1| putative reverse transcriptase family member [Mal... 53 1e-07
ref|XP_008354068.1| PREDICTED: uncharacterized protein LOC103417... 53 1e-07
gb|AAT38756.2| Putative methyltransferase family protein [Solanu... 42 2e-07
ref|XP_010694888.1| PREDICTED: uncharacterized protein LOC104907... 46 2e-07
ref|XP_010681189.1| PREDICTED: uncharacterized protein LOC104896... 48 2e-07
ref|XP_009605667.1| PREDICTED: uncharacterized protein LOC104100... 46 2e-07
gb|ABB00038.1| reverse transcriptase family member [Glycine max] 47 3e-07
ref|XP_010236203.1| PREDICTED: uncharacterized protein LOC104583... 47 7e-07
ref|XP_009783549.1| PREDICTED: uncharacterized protein LOC104232... 46 7e-07
emb|CDJ93072.1| RNA-directed DNA polymerase (reverse transcripta... 44 2e-06
gb|ADI61822.1| endonuclease-reverse transcriptase [Bombyx mori] 39 3e-06
gb|EMT05664.1| hypothetical protein F775_16727 [Aegilops tauschii] 44 3e-06
ref|XP_006361364.1| PREDICTED: uncharacterized protein LOC102596... 43 3e-06
>emb|CCH50976.1| T4.15 [Malus x robusta]
Length = 986
Score = 54.7 bits (130), Expect(2) = 4e-09
Identities = 29/55 (52%), Positives = 34/55 (61%)
Frame = +2
Query: 47 VRWMSGKIRKD*KKNGHI*GIVKVAPI*DKMRENHLSWFQHVTPRPSCAPV*KSD 211
+RWM G RKD +N I G V VA I KMREN L WF HV RP+ AP+ + D
Sbjct: 879 LRWMCGHTRKDKIRNEDIRGKVGVAEIQGKMRENQLRWFGHVQRRPTDAPIRRCD 933
Score = 32.7 bits (73), Expect(2) = 4e-09
Identities = 15/44 (34%), Positives = 26/44 (59%)
Frame = +1
Query: 235 RGRE*PKLAWKEIVRNDIVLC*VIEDNILNQAEWRNTIHEQNPT 366
RGR P+ +E +R D+ + +D ++A+WR+ IH +PT
Sbjct: 942 RGRGRPRKTLEETLRKDLEYLDLTKDMTQDRAQWRSKIHIADPT 985
>ref|WP_051127975.1| hypothetical protein [Bacillus isronensis]
Length = 116
Score = 52.4 bits (124), Expect(2) = 7e-09
Identities = 28/55 (50%), Positives = 33/55 (60%)
Frame = +2
Query: 47 VRWMSGKIRKD*KKNGHI*GIVKVAPI*DKMRENHLSWFQHVTPRPSCAPV*KSD 211
+RWM G RKD +N I G V VA I KMREN L WF HV P+ AP+ + D
Sbjct: 9 LRWMCGHTRKDKIRNEDIRGKVGVAEIEGKMRENRLRWFGHVQRMPTDAPIRRCD 63
Score = 34.3 bits (77), Expect(2) = 7e-09
Identities = 16/44 (36%), Positives = 26/44 (59%)
Frame = +1
Query: 235 RGRE*PKLAWKEIVRNDIVLC*VIEDNILNQAEWRNTIHEQNPT 366
RGR P+ +E +R D+ + ED ++A+WR+ IH +PT
Sbjct: 72 RGRGRPRKTLEETLRKDLEYLDLTEDMTQDRAQWRSKIHIADPT 115
>gb|EKB43273.1| hypothetical protein B857_03969 [Bacillus isronensis B3W22]
Length = 109
Score = 52.4 bits (124), Expect(2) = 8e-09
Identities = 28/55 (50%), Positives = 33/55 (60%)
Frame = +2
Query: 47 VRWMSGKIRKD*KKNGHI*GIVKVAPI*DKMRENHLSWFQHVTPRPSCAPV*KSD 211
+RWM G RKD +N I G V VA I KMREN L WF HV P+ AP+ + D
Sbjct: 2 LRWMCGHTRKDKIRNEDIRGKVGVAEIEGKMRENRLRWFGHVQRMPTDAPIRRCD 56
Score = 34.3 bits (77), Expect(2) = 8e-09
Identities = 16/44 (36%), Positives = 26/44 (59%)
Frame = +1
Query: 235 RGRE*PKLAWKEIVRNDIVLC*VIEDNILNQAEWRNTIHEQNPT 366
RGR P+ +E +R D+ + ED ++A+WR+ IH +PT
Sbjct: 65 RGRGRPRKTLEETLRKDLEYLDLTEDMTQDRAQWRSKIHIADPT 108
>gb|AAT40504.2| Polyprotein, putative [Solanum demissum]
Length = 868
Score = 45.4 bits (106), Expect(2) = 2e-08
Identities = 24/60 (40%), Positives = 36/60 (60%)
Frame = +2
Query: 47 VRWMSGKIRKD*KKNGHI*GIVKVAPI*DKMRENHLSWFQHVTPRPSCAPV*KSDLIILK 226
+RWM G R D +N I V VA + DK+RE L WF HV R + APV + ++++++
Sbjct: 587 LRWMCGHTRSDKIRNEVIREKVGVASVVDKLREARLRWFGHVKRRSADAPVRRCEVMVVE 646
Score = 39.7 bits (91), Expect(2) = 2e-08
Identities = 16/38 (42%), Positives = 25/38 (65%)
Frame = +1
Query: 235 RGRE*PKLAWKEIVRNDIVLC*VIEDNILNQAEWRNTI 348
RGR PK W+E++R D+ + + ED L++ EWR+ I
Sbjct: 650 RGRGRPKKYWEEVIRQDLAMLHITEDMTLDRKEWRSRI 687
>gb|KHN04075.1| hypothetical protein glysoja_036672 [Glycine soja]
Length = 110
Score = 50.4 bits (119), Expect(2) = 3e-08
Identities = 26/46 (56%), Positives = 30/46 (65%)
Frame = +2
Query: 47 VRWMSGKIRKD*KKNGHI*GIVKVAPI*DKMRENHLSWFQHVTPRP 184
+RWM GK R+D +NG I V VAPI +KM EN L WF HV RP
Sbjct: 4 LRWMCGKTRQDKIRNGAIRERVGVAPIVEKMVENRLRWFGHVERRP 49
Score = 34.3 bits (77), Expect(2) = 3e-08
Identities = 15/45 (33%), Positives = 26/45 (57%)
Frame = +1
Query: 232 VRGRE*PKLAWKEIVRNDIVLC*VIEDNILNQAEWRNTIHEQNPT 366
+RGR PK +E+++ D+ L + +L++ WR IH +PT
Sbjct: 66 IRGRGRPKKTIREVIKKDLELNDLDRSMVLDRTLWRKLIHVADPT 110
>ref|XP_010684010.1| PREDICTED: uncharacterized protein LOC104898613 [Beta vulgaris
subsp. vulgaris]
Length = 357
Score = 48.1 bits (113), Expect(2) = 3e-08
Identities = 27/51 (52%), Positives = 30/51 (58%)
Frame = +2
Query: 47 VRWMSGKIRKD*KKNGHI*GIVKVAPI*DKMRENHLSWFQHVTPRPSCAPV 199
+RWM G RKD +N I V VA I +KMREN L WF HV R APV
Sbjct: 251 LRWMCGHTRKDRLRNEVIREKVGVASIEEKMRENRLRWFGHVKRRSGDAPV 301
Score = 36.2 bits (82), Expect(2) = 3e-08
Identities = 15/44 (34%), Positives = 27/44 (61%)
Frame = +1
Query: 220 SQDIVRGRE*PKLAWKEIVRNDIVLC*VIEDNILNQAEWRNTIH 351
S IV+GR PK+ W ++ +D+ L + E L+++ WR +I+
Sbjct: 309 SNQIVKGRGRPKMTWLRVIESDMRLLGIEESMTLDRSGWRGSIY 352
>ref|WP_009187176.1| hypothetical protein [Cecembia lonarensis]
gi|405551390|gb|EKB47241.1| hypothetical protein
B879_04163 [Cecembia lonarensis LW9]
Length = 133
Score = 50.1 bits (118), Expect(2) = 3e-08
Identities = 29/55 (52%), Positives = 33/55 (60%)
Frame = +2
Query: 47 VRWMSGKIRKD*KKNGHI*GIVKVAPI*DKMRENHLSWFQHVTPRPSCAPV*KSD 211
+R M G RKD +N I G V VA I KMREN L WF HV RP+ APV + D
Sbjct: 26 LRGMCGHTRKDKIRNEDIQGKVGVAEIEGKMRENRLRWFGHVQRRPTDAPVRRCD 80
Score = 34.3 bits (77), Expect(2) = 3e-08
Identities = 16/44 (36%), Positives = 26/44 (59%)
Frame = +1
Query: 235 RGRE*PKLAWKEIVRNDIVLC*VIEDNILNQAEWRNTIHEQNPT 366
RGR P+ +E +R D+ + ED ++A+WR+ IH +PT
Sbjct: 89 RGRGRPRKTLEETLRKDLEYLDLTEDMTQDRAQWRSRIHIADPT 132
>gb|AEJ72569.1| putative reverse transcriptase family member [Malus domestica]
Length = 212
Score = 53.1 bits (126), Expect(2) = 1e-07
Identities = 28/55 (50%), Positives = 33/55 (60%)
Frame = +2
Query: 47 VRWMSGKIRKD*KKNGHI*GIVKVAPI*DKMRENHLSWFQHVTPRPSCAPV*KSD 211
+RWM G RKD +N I G V VA I MREN L WF HV RP+ AP+ + D
Sbjct: 105 LRWMCGHTRKDKIRNEDIRGKVGVAEIEGNMRENRLRWFGHVQRRPTYAPIRRCD 159
Score = 29.3 bits (64), Expect(2) = 1e-07
Identities = 14/44 (31%), Positives = 25/44 (56%)
Frame = +1
Query: 235 RGRE*PKLAWKEIVRNDIVLC*VIEDNILNQAEWRNTIHEQNPT 366
RGR P+ +E +R D+ + +D ++A+W + IH +PT
Sbjct: 168 RGRGRPRKTLEETLRKDLGYLDLTKDMTQDRAQWCSKIHIADPT 211
>ref|XP_008354068.1| PREDICTED: uncharacterized protein LOC103417687 [Malus domestica]
Length = 907
Score = 52.8 bits (125), Expect(2) = 1e-07
Identities = 28/55 (50%), Positives = 33/55 (60%)
Frame = +2
Query: 47 VRWMSGKIRKD*KKNGHI*GIVKVAPI*DKMRENHLSWFQHVTPRPSCAPV*KSD 211
+RWM G RKD +N I G V VA I KMREN L WF HV RP+ P+ + D
Sbjct: 800 LRWMCGHTRKDKIRNEDIRGKVGVAEIEGKMRENRLRWFGHVQRRPTDXPIRRCD 854
Score = 29.3 bits (64), Expect(2) = 1e-07
Identities = 13/44 (29%), Positives = 23/44 (52%)
Frame = +1
Query: 235 RGRE*PKLAWKEIVRNDIVLC*VIEDNILNQAEWRNTIHEQNPT 366
RGR P +E +R D+ + + ++ +WR+ IH +PT
Sbjct: 863 RGRGRPXKTLEETLRKDLEYLDLTXBMTXDRXQWRSKIHXADPT 906
>gb|AAT38756.2| Putative methyltransferase family protein [Solanum demissum]
Length = 828
Score = 42.4 bits (98), Expect(2) = 2e-07
Identities = 23/60 (38%), Positives = 35/60 (58%)
Frame = +2
Query: 47 VRWMSGKIRKD*KKNGHI*GIVKVAPI*DKMRENHLSWFQHVTPRPSCAPV*KSDLIILK 226
+RWM G R D +N I V VA + DK+RE L WF HV R + A V + ++++++
Sbjct: 4 LRWMCGHTRSDKIRNEVIREKVGVASVVDKLREARLRWFGHVKRRSADAAVRRCEVMVVE 63
Score = 39.7 bits (91), Expect(2) = 2e-07
Identities = 16/38 (42%), Positives = 25/38 (65%)
Frame = +1
Query: 235 RGRE*PKLAWKEIVRNDIVLC*VIEDNILNQAEWRNTI 348
RGR PK W+E++R D+ + + ED L++ EWR+ I
Sbjct: 67 RGRGRPKKYWEEVIRQDLAMLHITEDMTLDRKEWRSRI 104
>ref|XP_010694888.1| PREDICTED: uncharacterized protein LOC104907627 [Beta vulgaris
subsp. vulgaris]
Length = 292
Score = 46.2 bits (108), Expect(2) = 2e-07
Identities = 27/51 (52%), Positives = 29/51 (56%)
Frame = +2
Query: 47 VRWMSGKIRKD*KKNGHI*GIVKVAPI*DKMRENHLSWFQHVTPRPSCAPV 199
+RWM G RKD +N I V VA I KMREN L WF HV R APV
Sbjct: 186 LRWMCGHTRKDRLRNEVIREKVGVAYIEGKMRENRLRWFGHVKRRSGDAPV 236
Score = 35.8 bits (81), Expect(2) = 2e-07
Identities = 15/44 (34%), Positives = 26/44 (59%)
Frame = +1
Query: 220 SQDIVRGRE*PKLAWKEIVRNDIVLC*VIEDNILNQAEWRNTIH 351
S IV+GR PK+ W ++ +D+ L + E L++ WR +I+
Sbjct: 244 SNQIVKGRGRPKMTWLRVIESDMRLLGIEESMTLDRTGWRGSIY 287
>ref|XP_010681189.1| PREDICTED: uncharacterized protein LOC104896168 [Beta vulgaris
subsp. vulgaris]
Length = 176
Score = 48.1 bits (113), Expect(2) = 2e-07
Identities = 27/51 (52%), Positives = 30/51 (58%)
Frame = +2
Query: 47 VRWMSGKIRKD*KKNGHI*GIVKVAPI*DKMRENHLSWFQHVTPRPSCAPV 199
+RWM G RKD +N I V VA I +KMREN L WF HV R APV
Sbjct: 70 LRWMCGHTRKDRLRNEVIREKVGVASIEEKMRENRLRWFGHVKRRSGDAPV 120
Score = 33.9 bits (76), Expect(2) = 2e-07
Identities = 13/44 (29%), Positives = 27/44 (61%)
Frame = +1
Query: 220 SQDIVRGRE*PKLAWKEIVRNDIVLC*VIEDNILNQAEWRNTIH 351
S IV+G+ PK+ W ++ +D+ L + + L+++ WR +I+
Sbjct: 128 SNQIVKGKGRPKMTWLRVIESDMRLLGIEDSMTLDRSGWRGSIY 171
>ref|XP_009605667.1| PREDICTED: uncharacterized protein LOC104100193 [Nicotiana
tomentosiformis]
Length = 161
Score = 46.2 bits (108), Expect(2) = 2e-07
Identities = 25/51 (49%), Positives = 30/51 (58%)
Frame = +2
Query: 47 VRWMSGKIRKD*KKNGHI*GIVKVAPI*DKMRENHLSWFQHVTPRPSCAPV 199
+RWM G R D +N I V VAP+ DKMRE L WF H+ R + APV
Sbjct: 57 LRWMCGHTRMDKIRNEDIREKVGVAPMEDKMREVRLRWFGHIQRRSTDAPV 107
Score = 35.4 bits (80), Expect(2) = 2e-07
Identities = 15/38 (39%), Positives = 24/38 (63%)
Frame = +1
Query: 235 RGRE*PKLAWKEIVRNDIVLC*VIEDNILNQAEWRNTI 348
RGR PK W+E++R D+ + ED L++ WR++I
Sbjct: 120 RGRGRPKKYWEEVIRQDMARLRITEDMALDRELWRSSI 157
>gb|ABB00038.1| reverse transcriptase family member [Glycine max]
Length = 377
Score = 47.4 bits (111), Expect(2) = 3e-07
Identities = 25/46 (54%), Positives = 29/46 (63%)
Frame = +2
Query: 47 VRWMSGKIRKD*KKNGHI*GIVKVAPI*DKMRENHLSWFQHVTPRP 184
+RWM GK R+D +N I V VAPI +KM EN L WF HV RP
Sbjct: 271 LRWMCGKTRQDKIRNEAIRERVGVAPIVEKMVENRLRWFGHVERRP 316
Score = 33.5 bits (75), Expect(2) = 3e-07
Identities = 14/45 (31%), Positives = 26/45 (57%)
Frame = +1
Query: 232 VRGRE*PKLAWKEIVRNDIVLC*VIEDNILNQAEWRNTIHEQNPT 366
+RGR PK +E+++ D+ + + +L++ WR IH +PT
Sbjct: 333 IRGRGRPKKTIREVIKKDLEINGLDRSMVLDRTLWRKLIHVADPT 377
>ref|XP_010236203.1| PREDICTED: uncharacterized protein LOC104583892 [Brachypodium
distachyon]
Length = 429
Score = 46.6 bits (109), Expect(2) = 7e-07
Identities = 23/51 (45%), Positives = 31/51 (60%)
Frame = +2
Query: 47 VRWMSGKIRKD*KKNGHI*GIVKVAPI*DKMRENHLSWFQHVTPRPSCAPV 199
+RWM G R+D +N I V VAPI +K+ ++ L WF H+ RP APV
Sbjct: 324 LRWMCGHTRRDRVRNDDIRDRVGVAPIAEKLVQHRLRWFGHIQRRPPDAPV 374
Score = 33.1 bits (74), Expect(2) = 7e-07
Identities = 14/46 (30%), Positives = 26/46 (56%)
Frame = +1
Query: 226 DIVRGRE*PKLAWKEIVRNDIVLC*VIEDNILNQAEWRNTIHEQNP 363
++ RGR P L W+E V+ D+ + ++ +++A W+ IH P
Sbjct: 384 NVKRGRGRPNLTWEESVKRDLKGWNITKELAMDRAAWKLAIHVPEP 429
>ref|XP_009783549.1| PREDICTED: uncharacterized protein LOC104232135 [Nicotiana
sylvestris]
Length = 232
Score = 46.2 bits (108), Expect(2) = 7e-07
Identities = 26/59 (44%), Positives = 32/59 (54%)
Frame = +2
Query: 47 VRWMSGKIRKD*KKNGHI*GIVKVAPI*DKMRENHLSWFQHVTPRPSCAPV*KSDLIIL 223
+RWM G R D +N I V VAP+ DKMRE WF HV R APV + + + L
Sbjct: 128 LRWMCGHTRLDKIRNEDIRERVSVAPVDDKMREARFRWFGHVRRRSLDAPVRRCERLTL 186
Score = 33.5 bits (75), Expect(2) = 7e-07
Identities = 15/38 (39%), Positives = 22/38 (57%)
Frame = +1
Query: 235 RGRE*PKLAWKEIVRNDIVLC*VIEDNILNQAEWRNTI 348
RGR PK W E++R D+ + ED L++ WR+ I
Sbjct: 191 RGRGRPKKYWGEVIRQDMARLQISEDMALDRNMWRSNI 228
>emb|CDJ93072.1| RNA-directed DNA polymerase (reverse transcriptase) domain
containing protein [Haemonchus contortus]
Length = 289
Score = 43.9 bits (102), Expect(2) = 2e-06
Identities = 21/54 (38%), Positives = 31/54 (57%)
Frame = +2
Query: 47 VRWMSGKIRKD*KKNGHI*GIVKVAPI*DKMRENHLSWFQHVTPRPSCAPV*KS 208
+RW G R D +N + ++++AP+ KMRE L WF HV RP P+ K+
Sbjct: 180 LRWTCGWTRLDRIRNEAVRTVMQIAPVQLKMREQRLRWFGHVLRRPQSHPIRKA 233
Score = 34.3 bits (77), Expect(2) = 2e-06
Identities = 13/45 (28%), Positives = 25/45 (55%)
Frame = +1
Query: 235 RGRE*PKLAWKEIVRNDIVLC*VIEDNILNQAEWRNTIHEQNPTT 369
R R PK W+++++ D+ V ++ +N+ +WR I +P T
Sbjct: 242 RPRGAPKKRWRDVIKKDLAEAKVTAEDAVNRMKWRRLIRTADPAT 286
>gb|ADI61822.1| endonuclease-reverse transcriptase [Bombyx mori]
Length = 996
Score = 39.3 bits (90), Expect(2) = 3e-06
Identities = 19/44 (43%), Positives = 27/44 (61%)
Frame = +2
Query: 41 KDVRWMSGKIRKD*KKNGHI*GIVKVAPI*DKMRENHLSWFQHV 172
K +RWM G R D +N ++ G + V I DKM+E+ L W+ HV
Sbjct: 884 KMLRWMCGVTRLDKIRNEYVRGSLGVRDIADKMQESRLRWYGHV 927
Score = 38.5 bits (88), Expect(2) = 3e-06
Identities = 17/48 (35%), Positives = 32/48 (66%), Gaps = 1/48 (2%)
Frame = +1
Query: 235 RGRE*PKLAWKEIVRNDIVLC*VIEDNILNQAEWRNTIHEQNPTT-WE 375
RGR PK W+++V D C V ++++ ++A+WR+ + + +PTT W+
Sbjct: 950 RGR--PKTRWRDVVLKDKRECNVADEDVEDRAKWRSKVRKADPTTMWD 995
>gb|EMT05664.1| hypothetical protein F775_16727 [Aegilops tauschii]
Length = 650
Score = 44.3 bits (103), Expect(2) = 3e-06
Identities = 23/51 (45%), Positives = 31/51 (60%)
Frame = +2
Query: 47 VRWMSGKIRKD*KKNGHI*GIVKVAPI*DKMRENHLSWFQHVTPRPSCAPV 199
+RWM G +RKD +N I V API +K+ ++ L WF H+ RP APV
Sbjct: 545 LRWMCGHMRKDRVRNDDIRDRVGGAPIEEKLVQHCLRWFGHIQRRPPEAPV 595
Score = 33.5 bits (75), Expect(2) = 3e-06
Identities = 14/48 (29%), Positives = 27/48 (56%)
Frame = +1
Query: 220 SQDIVRGRE*PKLAWKEIVRNDIVLC*VIEDNILNQAEWRNTIHEQNP 363
++++ RGR P L W+E V+ D+ + ++ L++ W+ IH P
Sbjct: 603 AENVKRGRGRPNLTWEESVKRDLKDWSITKELALDRGAWKLAIHVPEP 650
>ref|XP_006361364.1| PREDICTED: uncharacterized protein LOC102596609 [Solanum tuberosum]
Length = 278
Score = 43.1 bits (100), Expect(2) = 3e-06
Identities = 24/60 (40%), Positives = 35/60 (58%)
Frame = +2
Query: 47 VRWMSGKIRKD*KKNGHI*GIVKVAPI*DKMRENHLSWFQHVTPRPSCAPV*KSDLIILK 226
+RWM G R D +N I V VA + DK+RE L WF HV R + APV + + ++++
Sbjct: 174 LRWMCGHTRSDKIRNEVIREKVGVASVVDKLREARLRWFGHVRRRCADAPVRRCEGLVVE 233
Score = 34.7 bits (78), Expect(2) = 3e-06
Identities = 15/38 (39%), Positives = 22/38 (57%)
Frame = +1
Query: 235 RGRE*PKLAWKEIVRNDIVLC*VIEDNILNQAEWRNTI 348
RGR PK W E++R D+ + ED L++ WR+ I
Sbjct: 237 RGRGRPKKYWGEVIRQDLAQLHITEDMTLDRKAWRSRI 274