BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Aconitum23_contig00036798
         (1192 letters)

Database: ./nr 
           77,306,371 sequences; 28,104,191,420 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcocla...   531   e-148
gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chi...   525   e-146
gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum...   510   e-141
gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase ...   472   e-130
sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-...   471   e-130
sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-...   471   e-130
gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [P...   468   e-129
sp|Q9SP06.1|C80B3_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-...   466   e-128
gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [...   464   e-128
sp|I3V6B1.1|C80BX_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-...   393   e-106
ref|XP_010253990.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   341   6e-91
ref|XP_012076022.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   328   4e-87
gb|KDP20161.1| hypothetical protein JCGZ_00026 [Jatropha curcas]...   328   4e-87
ref|XP_002283415.2| PREDICTED: probable (S)-N-methylcoclaurine 3...   326   3e-86
emb|CBI34715.3| unnamed protein product [Vitis vinifera]              325   4e-86
ref|XP_002510830.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym...   324   8e-86
ref|XP_002283461.2| PREDICTED: probable (S)-N-methylcoclaurine 3...   323   1e-85
emb|CBI34714.3| unnamed protein product [Vitis vinifera]              323   1e-85
ref|XP_011041604.1| PREDICTED: probable (S)-N-methylcoclaurine 3...   322   3e-85
gb|AJD20229.1| corytuberine synthase [Sinopodophyllum hexandrum]      322   5e-85

>sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
            2; AltName: Full=Cytochrome P450 80B2 [Coptis japonica]
            gi|9971208|dbj|BAB12433.1|
            (S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
          Length = 488

 Score =  531 bits (1367), Expect = e-148
 Identities = 269/360 (74%), Positives = 300/360 (83%), Gaps = 1/360 (0%)
 Frame = -1

Query: 1192 RTELFTPKMIESQAYIREDKALDMVKYLRXXXXXXXXXXXXVFGTLVNIFGNLIFSKDVF 1013
            RTELFTPKMIESQ+ IRE KA +MVK+LR            VFGTLVNIFGNLIFSKDVF
Sbjct: 129  RTELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVVFGTLVNIFGNLIFSKDVF 188

Query: 1012 ELGEGSRDGGSAEMKVLLWKLLEMGNSTNLADYFPVLGSLDLFGQRRAVAEVLKQIYDVW 833
            +L + +  GGS E+K  LWKLL+MGNSTN ADYFP++G LDLFGQRRAVAEVL+QIYDVW
Sbjct: 189  DLEDPT--GGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVLQQIYDVW 246

Query: 832  GAMLKERRGAKG-DCRNDFVNVLIDSGLDDQKINALLMELFGAGTETSASTIEWAIAELT 656
            G MLKERRG KG + +NDFV+VL+++GLDDQKINALLMELFGAGTETSASTIEWAI ELT
Sbjct: 247  GVMLKERRGTKGSESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSASTIEWAITELT 306

Query: 655  KNPRITSKLQSELQNIVGENTVKESDIPHLPYLQAFVKEXXXXXXXXXXXXXXXXLETCT 476
            K P + SK++ EL N+VG+NTVKESD+PHLPYLQAFVKE                LETCT
Sbjct: 307  KKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETLRLHPPTPLLLPRRALETCT 366

Query: 475  VMNYTIPKDCQIMVNAWAIGRDPKTWDEPLRFKPERFLSCGVDFKGNDFELIPFGGGRRI 296
            VMNYTIPK+CQIMVNAWAIGRDPKTWD+PL FKPERFLS  VD+KGNDFELIPFGGGRRI
Sbjct: 367  VMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPFGGGRRI 426

Query: 295  CPGLPLASQFISLIVATFVQNFEWRLPDGVSSDELSMEEKFGLTLQKDPPLLIVTRARGS 116
            CPGLPLASQF +LIVAT VQNFEW LP G+S+ ELSM+EKFGLTLQKDPPLLIV +AR S
Sbjct: 427  CPGLPLASQFSNLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIVLKARAS 486


>gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
          Length = 488

 Score =  525 bits (1351), Expect = e-146
 Identities = 267/360 (74%), Positives = 297/360 (82%), Gaps = 1/360 (0%)
 Frame = -1

Query: 1192 RTELFTPKMIESQAYIREDKALDMVKYLRXXXXXXXXXXXXVFGTLVNIFGNLIFSKDVF 1013
            RTELFTPKMIESQ+ IRE KA +MVK+LR            VFGTLVNIFGNLIFSKDVF
Sbjct: 129  RTELFTPKMIESQSEIREGKAREMVKFLRSKEGEVVKIVEVVFGTLVNIFGNLIFSKDVF 188

Query: 1012 ELGEGSRDGGSAEMKVLLWKLLEMGNSTNLADYFPVLGSLDLFGQRRAVAEVLKQIYDVW 833
            +L + +  GGS E+K  LWKLLEMGNSTN ADYFP++G  DLFGQRRAVA+VL+QIYDVW
Sbjct: 189  DLEDPT--GGSVELKEHLWKLLEMGNSTNPADYFPIMGKYDLFGQRRAVADVLQQIYDVW 246

Query: 832  GAMLKERRGAKG-DCRNDFVNVLIDSGLDDQKINALLMELFGAGTETSASTIEWAIAELT 656
            G  LKERRG KG +  NDFV+VL+++GL+DQKINALLMELFG GTETSASTIEWAI ELT
Sbjct: 247  GINLKERRGMKGSESNNDFVDVLLNAGLEDQKINALLMELFGTGTETSASTIEWAITELT 306

Query: 655  KNPRITSKLQSELQNIVGENTVKESDIPHLPYLQAFVKEXXXXXXXXXXXXXXXXLETCT 476
            K P + SK++ EL N+VG+NTVKESD+PHLPYLQAFVKE                LETCT
Sbjct: 307  KKPLVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETLRLHPPTPLLLPRRALETCT 366

Query: 475  VMNYTIPKDCQIMVNAWAIGRDPKTWDEPLRFKPERFLSCGVDFKGNDFELIPFGGGRRI 296
            VMNYTIPK+CQIMVNAWAIGRDPKTWD+PL FKPERFLS  VD+KGNDFELIPFGGGRRI
Sbjct: 367  VMNYTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPFGGGRRI 426

Query: 295  CPGLPLASQFISLIVATFVQNFEWRLPDGVSSDELSMEEKFGLTLQKDPPLLIVTRARGS 116
            CPGLPLASQFISLIVAT VQNFEW LP G+S+ ELSM+EKFGLTLQKDPPLLIV +AR S
Sbjct: 427  CPGLPLASQFISLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIVLKARPS 486


>gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum flavum subsp.
            glaucum]
          Length = 491

 Score =  510 bits (1313), Expect = e-141
 Identities = 257/360 (71%), Positives = 291/360 (80%)
 Frame = -1

Query: 1192 RTELFTPKMIESQAYIREDKALDMVKYLRXXXXXXXXXXXXVFGTLVNIFGNLIFSKDVF 1013
            RTELF+PKMIESQAYIRE KALDMV++LR            VF TLVNIFGNLIFSKDVF
Sbjct: 130  RTELFSPKMIESQAYIREAKALDMVRFLRKKENQEVKIVEVVFNTLVNIFGNLIFSKDVF 189

Query: 1012 ELGEGSRDGGSAEMKVLLWKLLEMGNSTNLADYFPVLGSLDLFGQRRAVAEVLKQIYDVW 833
            +L + +  GGSAE+K  LWKLL+MGNSTN ADYFP++G  DLFGQRR VA+VLKQIYDVW
Sbjct: 190  DLEDPT--GGSAELKEHLWKLLDMGNSTNPADYFPIMGKFDLFGQRREVAKVLKQIYDVW 247

Query: 832  GAMLKERRGAKGDCRNDFVNVLIDSGLDDQKINALLMELFGAGTETSASTIEWAIAELTK 653
            G MLKERR   G   NDF NVL+++GLDDQKINALLMELFGAGTET+ASTIEWAI ELT 
Sbjct: 248  GVMLKERRSITGHRENDFANVLLNAGLDDQKINALLMELFGAGTETTASTIEWAITELTN 307

Query: 652  NPRITSKLQSELQNIVGENTVKESDIPHLPYLQAFVKEXXXXXXXXXXXXXXXXLETCTV 473
            N R+ SKL++EL N+VG  TVKES+IPHLPYLQA VKE                LETC V
Sbjct: 308  NLRVISKLRAELINVVGHKTVKESNIPHLPYLQAIVKETLRLHPPTPLLLPRRALETCKV 367

Query: 472  MNYTIPKDCQIMVNAWAIGRDPKTWDEPLRFKPERFLSCGVDFKGNDFELIPFGGGRRIC 293
            MNYTIPK+CQIMVNAWAIGRDPKTWD+PL FKPERF++  VD+KGNDFELIPFGGGRRIC
Sbjct: 368  MNYTIPKECQIMVNAWAIGRDPKTWDDPLTFKPERFMNSTVDYKGNDFELIPFGGGRRIC 427

Query: 292  PGLPLASQFISLIVATFVQNFEWRLPDGVSSDELSMEEKFGLTLQKDPPLLIVTRARGSI 113
            PGLPLASQF+SLIVAT VQNF+W  P G++ +E+ M+EKFGL LQKDPPLLIV +AR S+
Sbjct: 428  PGLPLASQFLSLIVATLVQNFDWSFPQGMTPNEVPMDEKFGLPLQKDPPLLIVLKARTSM 487


>gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase from Eschscholzia
            californica [Expression vector pWCD2353]
          Length = 490

 Score =  472 bits (1214), Expect = e-130
 Identities = 236/361 (65%), Positives = 284/361 (78%), Gaps = 4/361 (1%)
 Frame = -1

Query: 1192 RTELFTPKMIESQAYIREDKALDMVKYLRXXXXXXXXXXXXVFGTLVNIFGNLIFSKDVF 1013
            RTELFT KMIESQA++RE K  +MV+YL             +FGTLVNIFGNLIFS+++F
Sbjct: 129  RTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQNIF 188

Query: 1012 ELGEGSRDGGSAEMKVLLWKLLEMGNSTNLADYFPVLGSLDLFGQRRAVAEVLKQIYDVW 833
            ELG+ +   GS+E K  LW++LE+GNSTN ADYFP+LG  DLFGQR+ VAE LK IY +W
Sbjct: 189  ELGDPN--SGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAIW 246

Query: 832  GAMLKERRGAKG----DCRNDFVNVLIDSGLDDQKINALLMELFGAGTETSASTIEWAIA 665
            GAML+ER+ AK       +NDFV+V +DSGL+D +INALLMELFGAGTETSASTIEWA+ 
Sbjct: 247  GAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAMT 306

Query: 664  ELTKNPRITSKLQSELQNIVGENTVKESDIPHLPYLQAFVKEXXXXXXXXXXXXXXXXLE 485
            ELTKNP+IT+KL+SELQ +VGE +VKESD P+LPYL+A VKE                LE
Sbjct: 307  ELTKNPKITAKLRSELQTVVGERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPRRALE 366

Query: 484  TCTVMNYTIPKDCQIMVNAWAIGRDPKTWDEPLRFKPERFLSCGVDFKGNDFELIPFGGG 305
            TCT++NYTIPKDCQIMVNAW IGRDPKTW +PL F PERFL+  VDF+GNDF LIPFG G
Sbjct: 367  TCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFLNSSVDFRGNDFSLIPFGAG 426

Query: 304  RRICPGLPLASQFISLIVATFVQNFEWRLPDGVSSDELSMEEKFGLTLQKDPPLLIVTRA 125
            RRICPGLP+A+QFI+L+VATFVQN +W LP+G+S D L +EEKFGLTLQK+PPL IV ++
Sbjct: 427  RRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFIVPKS 486

Query: 124  R 122
            R
Sbjct: 487  R 487


>sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
            AltName: Full=Cytochrome P450 80B1
            gi|3127027|gb|AAC39452.1| (S)-N-methylcoclaurine
            3'-hydroxylase [Eschscholzia californica]
          Length = 487

 Score =  471 bits (1211), Expect = e-130
 Identities = 234/361 (64%), Positives = 284/361 (78%), Gaps = 4/361 (1%)
 Frame = -1

Query: 1192 RTELFTPKMIESQAYIREDKALDMVKYLRXXXXXXXXXXXXVFGTLVNIFGNLIFSKDVF 1013
            RTELFT KMIESQA++RE K  +MV+YL             +FGTLVNIFGNLIFS+++F
Sbjct: 128  RTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQNIF 187

Query: 1012 ELGEGSRDGGSAEMKVLLWKLLEMGNSTNLADYFPVLGSLDLFGQRRAVAEVLKQIYDVW 833
            ELG+ +   GS+E K  LW++LE+GNSTN ADYFP+LG  DLFGQR+ VAE LK IY +W
Sbjct: 188  ELGDPN--SGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAIW 245

Query: 832  GAMLKERRGAKG----DCRNDFVNVLIDSGLDDQKINALLMELFGAGTETSASTIEWAIA 665
            GAML+ER+ AK       +NDFV+V +DSGL+D +INALLMELFGAGTETSASTIEWA+ 
Sbjct: 246  GAMLQERKLAKKVDGYKSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAMT 305

Query: 664  ELTKNPRITSKLQSELQNIVGENTVKESDIPHLPYLQAFVKEXXXXXXXXXXXXXXXXLE 485
            ELTKNP+IT+K++SE+Q +VGE +VKESD P+LPYL+A VKE                LE
Sbjct: 306  ELTKNPKITAKIRSEIQTVVGERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPRRALE 365

Query: 484  TCTVMNYTIPKDCQIMVNAWAIGRDPKTWDEPLRFKPERFLSCGVDFKGNDFELIPFGGG 305
            TCT++NYTIPKDCQIMVNAW IGRDPKTW +PL F PERFL+  VDF+GNDF LIPFG G
Sbjct: 366  TCTILNYTIPKDCQIMVNAWGIGRDPKTWTDPLTFSPERFLNSSVDFRGNDFSLIPFGAG 425

Query: 304  RRICPGLPLASQFISLIVATFVQNFEWRLPDGVSSDELSMEEKFGLTLQKDPPLLIVTRA 125
            RRICPGLP+A+QFI+L+VATFVQN +W LP+G+S D L +EEKFGLTLQK+PPL IV ++
Sbjct: 426  RRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFIVPKS 485

Query: 124  R 122
            R
Sbjct: 486  R 486


>sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2;
            AltName: Full=Cytochrome P450 80B2
            gi|3127029|gb|AAC39453.1| (S)-N-methylcoclaurine
            3'-hydroxylase [Eschscholzia californica]
          Length = 488

 Score =  471 bits (1211), Expect = e-130
 Identities = 236/361 (65%), Positives = 283/361 (78%), Gaps = 4/361 (1%)
 Frame = -1

Query: 1192 RTELFTPKMIESQAYIREDKALDMVKYLRXXXXXXXXXXXXVFGTLVNIFGNLIFSKDVF 1013
            RTELFT KMIESQA++RE K  +MV+YL             +FGTLVNIFGNLIFS+++F
Sbjct: 129  RTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQNIF 188

Query: 1012 ELGEGSRDGGSAEMKVLLWKLLEMGNSTNLADYFPVLGSLDLFGQRRAVAEVLKQIYDVW 833
            ELG  +   GS+E K  LW++LE+GNSTN ADYFP+LG  DLFGQR+ VAE LK IY +W
Sbjct: 189  ELGXPN--SGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAIW 246

Query: 832  GAMLKERRGAKG----DCRNDFVNVLIDSGLDDQKINALLMELFGAGTETSASTIEWAIA 665
            GAML+ER+ AK       +NDFV+V +DSGL+D +INALLMELFGAGTETSASTIEWA+ 
Sbjct: 247  GAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAMT 306

Query: 664  ELTKNPRITSKLQSELQNIVGENTVKESDIPHLPYLQAFVKEXXXXXXXXXXXXXXXXLE 485
            ELTKNP+IT+KL+SELQ +VGE +VKESD P+LPYL+A VKE                LE
Sbjct: 307  ELTKNPKITAKLRSELQTVVGERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPRRALE 366

Query: 484  TCTVMNYTIPKDCQIMVNAWAIGRDPKTWDEPLRFKPERFLSCGVDFKGNDFELIPFGGG 305
            TCT++NYTIPKDCQIMVNAW IGRDPKTW +PL F PERFL+  VDF+GNDF LIPFG G
Sbjct: 367  TCTILNYTIPKDCQIMVNAWGIGRDPKTWIDPLTFSPERFLNSSVDFRGNDFSLIPFGAG 426

Query: 304  RRICPGLPLASQFISLIVATFVQNFEWRLPDGVSSDELSMEEKFGLTLQKDPPLLIVTRA 125
            RRICPGLP+A+QFI+L+VATFVQN +W LP+G+S D L +EEKFGLTLQK+PPL IV ++
Sbjct: 427  RRICPGLPIANQFIALLVATFVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFIVPKS 486

Query: 124  R 122
            R
Sbjct: 487  R 487


>gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [Papaver bracteatum]
          Length = 486

 Score =  468 bits (1205), Expect = e-129
 Identities = 235/360 (65%), Positives = 281/360 (78%), Gaps = 3/360 (0%)
 Frame = -1

Query: 1192 RTELFTPKMIESQAYIREDKALDMVKYLRXXXXXXXXXXXXVFGTLVNIFGNLIFSKDVF 1013
            R ELFT KMIESQA IRE KA++MV++L+            VFGTLVNIFGNLIFS+++F
Sbjct: 128  RAELFTQKMIESQAEIRESKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFSQNIF 187

Query: 1012 ELGEGSRDGGSAEMKVLLWKLLEMGNSTNLADYFPVLGSLDLFGQRRAVAEVLKQIYDVW 833
            +LG+ S   GS EMK  LW++LE+GNSTN ADYFP LG  DLFGQR+ VA+ L+ IY VW
Sbjct: 188  KLGDES--SGSVEMKEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGIYSVW 245

Query: 832  GAMLKERRGAK---GDCRNDFVNVLIDSGLDDQKINALLMELFGAGTETSASTIEWAIAE 662
            GAMLKER+ AK      +NDFV +L+DSGLDDQ+INALLME+FGAGTETSASTIEWA++E
Sbjct: 246  GAMLKERKIAKLHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSE 305

Query: 661  LTKNPRITSKLQSELQNIVGENTVKESDIPHLPYLQAFVKEXXXXXXXXXXXXXXXXLET 482
            LTKNP +T+ ++SEL ++VG+  VKESDIP++PYLQAFVKE                LET
Sbjct: 306  LTKNPEVTANMRSELLSVVGKRPVKESDIPNMPYLQAFVKETLRLHPATPLLLPRRALET 365

Query: 481  CTVMNYTIPKDCQIMVNAWAIGRDPKTWDEPLRFKPERFLSCGVDFKGNDFELIPFGGGR 302
            C V+NYTIPK+CQIMVNAW IGRDPK W +PL+F PERFL+  +DFKGNDFELIPFG GR
Sbjct: 366  CKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFAPERFLNSSIDFKGNDFELIPFGAGR 425

Query: 301  RICPGLPLASQFISLIVATFVQNFEWRLPDGVSSDELSMEEKFGLTLQKDPPLLIVTRAR 122
            RICPG+PLA+QFISLIV T VQNF+W LP G+   +L MEEKFGLTLQK+PPL IV + R
Sbjct: 426  RICPGVPLATQFISLIVPTLVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPLYIVPKNR 485


>sp|Q9SP06.1|C80B3_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
            AltName: Full=Cytochrome P450 80B1; AltName:
            Full=Cytochrome P450 80B3
            gi|6176562|gb|AAF05621.1|AF191772_1
            (S)-N-methylcoclaurine 3'-hydroxylase [Papaver
            somniferum]
          Length = 481

 Score =  466 bits (1200), Expect = e-128
 Identities = 232/360 (64%), Positives = 281/360 (78%), Gaps = 3/360 (0%)
 Frame = -1

Query: 1192 RTELFTPKMIESQAYIREDKALDMVKYLRXXXXXXXXXXXXVFGTLVNIFGNLIFSKDVF 1013
            RTELFT KMIESQA +RE KA++MV+YL+            VFGTLVNIFGNLIFS+++F
Sbjct: 123  RTELFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIF 182

Query: 1012 ELGEGSRDGGSAEMKVLLWKLLEMGNSTNLADYFPVLGSLDLFGQRRAVAEVLKQIYDVW 833
            +LG+ S   GS EMK  LW++LE+GNSTN ADYFP LG  DLFGQ + VA+ L+ IY VW
Sbjct: 183  KLGDES--SGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVW 240

Query: 832  GAMLKERRGAK---GDCRNDFVNVLIDSGLDDQKINALLMELFGAGTETSASTIEWAIAE 662
            GAMLKER+ AK      +NDFV +L+DSGLDDQ+INALLME+FGAGTETSASTIEWA++E
Sbjct: 241  GAMLKERKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSE 300

Query: 661  LTKNPRITSKLQSELQNIVGENTVKESDIPHLPYLQAFVKEXXXXXXXXXXXXXXXXLET 482
            LTKNP++T+ ++ EL ++VG+  VKESDIP++PYLQAFVKE                LET
Sbjct: 301  LTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKETLRLHPATPLLLPRRALET 360

Query: 481  CTVMNYTIPKDCQIMVNAWAIGRDPKTWDEPLRFKPERFLSCGVDFKGNDFELIPFGGGR 302
            C V+NYTIPK+CQIMVNAW IGRDPK W +PL+F PERFL+  +DFKGNDFELIPFG GR
Sbjct: 361  CKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPFGAGR 420

Query: 301  RICPGLPLASQFISLIVATFVQNFEWRLPDGVSSDELSMEEKFGLTLQKDPPLLIVTRAR 122
            RICPG+PLA+QFISLIV++ VQNF+W  P G+   +L MEEKFGLTLQK+PPL IV + R
Sbjct: 421  RICPGVPLATQFISLIVSSLVQNFDWGFPKGMDPSQLIMEEKFGLTLQKEPPLYIVPKTR 480


>gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
          Length = 481

 Score =  464 bits (1195), Expect = e-128
 Identities = 231/360 (64%), Positives = 281/360 (78%), Gaps = 3/360 (0%)
 Frame = -1

Query: 1192 RTELFTPKMIESQAYIREDKALDMVKYLRXXXXXXXXXXXXVFGTLVNIFGNLIFSKDVF 1013
            RT+LFT KMIESQA +RE KA++MV+YL+            VFGTLVNIFGNLIFS+++F
Sbjct: 123  RTDLFTQKMIESQAEVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIF 182

Query: 1012 ELGEGSRDGGSAEMKVLLWKLLEMGNSTNLADYFPVLGSLDLFGQRRAVAEVLKQIYDVW 833
            +LG+ S   GS EMK  LW++LE+GNSTN ADYFP LG  DLFGQ + VA+ L+ IY VW
Sbjct: 183  KLGDES--SGSVEMKEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVW 240

Query: 832  GAMLKERRGAK---GDCRNDFVNVLIDSGLDDQKINALLMELFGAGTETSASTIEWAIAE 662
            GAMLKE + AK      +NDFV +L+DSGLDDQ+INALLME+FGAGTETSASTIEWA++E
Sbjct: 241  GAMLKESKIAKQHNNSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSE 300

Query: 661  LTKNPRITSKLQSELQNIVGENTVKESDIPHLPYLQAFVKEXXXXXXXXXXXXXXXXLET 482
            LTKNP++T+ ++ EL ++VG+  VKESDIP++PYLQAFVKE                LET
Sbjct: 301  LTKNPQVTANMRLELLSVVGKRPVKESDIPNMPYLQAFVKETLRLHPATPLLLPRRALET 360

Query: 481  CTVMNYTIPKDCQIMVNAWAIGRDPKTWDEPLRFKPERFLSCGVDFKGNDFELIPFGGGR 302
            C V+NYTIPK+CQIMVNAW IGRDPK W +PL+F PERFL+  +DFKGNDFELIPFG GR
Sbjct: 361  CKVLNYTIPKECQIMVNAWGIGRDPKRWTDPLKFSPERFLNSSIDFKGNDFELIPFGAGR 420

Query: 301  RICPGLPLASQFISLIVATFVQNFEWRLPDGVSSDELSMEEKFGLTLQKDPPLLIVTRAR 122
            RICPG+PLA+QFISLIV++ VQNF+W LP G+   +L MEEKFGLTLQK+PPL IV + R
Sbjct: 421  RICPGVPLATQFISLIVSSLVQNFDWGLPKGMDPSQLIMEEKFGLTLQKEPPLYIVPKTR 480


>sp|I3V6B1.1|C80BX_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase-like protein;
            AltName: Full=Cytochrome P450 80BX
            gi|388571234|gb|AFK73713.1| cytochrome P450 [Papaver
            somniferum]
          Length = 440

 Score =  393 bits (1010), Expect = e-106
 Identities = 193/308 (62%), Positives = 241/308 (78%), Gaps = 4/308 (1%)
 Frame = -1

Query: 1192 RTELFTPKMIESQAYIREDKALDMVKYLRXXXXXXXXXXXXVFGTLVNIFGNLIFSKDVF 1013
            RT+LFT KMIE+QA +RE K ++MVKYL+            +FGTLVN+FGNLIFS+D+F
Sbjct: 129  RTQLFTQKMIENQAEVREIKTMEMVKYLKKNEGIEVKIVEVIFGTLVNMFGNLIFSQDIF 188

Query: 1012 ELGEGSRDGGSAEMKVLLWKLLEMGNSTNLADYFPVLGSLDLFGQRRAVAEVLKQIYDVW 833
            +LG+ S   GS EMK  +W++LE+GNS N  DY P+LGSLDLFGQR+ VA+ L+ +Y VW
Sbjct: 189  KLGDES--SGSLEMKQHIWRMLELGNSANPGDYVPLLGSLDLFGQRKDVADCLQGVYGVW 246

Query: 832  GAMLKERRGAK----GDCRNDFVNVLIDSGLDDQKINALLMELFGAGTETSASTIEWAIA 665
            GAMLKERR AK    GD +NDFV+VL+DSGLDDQ+IN+LL+++F AGTET+ASTIEWA+ 
Sbjct: 247  GAMLKERRIAKRQINGDTKNDFVDVLLDSGLDDQQINSLLLDMFSAGTETTASTIEWALT 306

Query: 664  ELTKNPRITSKLQSELQNIVGENTVKESDIPHLPYLQAFVKEXXXXXXXXXXXXXXXXLE 485
            ELTKNP++T+ ++SEL ++VG+  VKESDI +LPYLQAFVKE                LE
Sbjct: 307  ELTKNPQVTADIRSELLSVVGKRAVKESDILNLPYLQAFVKETLRLHPPTPLLIPRRALE 366

Query: 484  TCTVMNYTIPKDCQIMVNAWAIGRDPKTWDEPLRFKPERFLSCGVDFKGNDFELIPFGGG 305
            TC V+NYTIPK+CQIMVNAW IGRDPKTW +PL+F P+RFL+  +DFKGNDFELIPFG G
Sbjct: 367  TCQVLNYTIPKECQIMVNAWGIGRDPKTWTDPLKFSPDRFLNSSIDFKGNDFELIPFGAG 426

Query: 304  RRICPGLP 281
            RRICPG+P
Sbjct: 427  RRICPGVP 434


>ref|XP_010253990.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
            [Nelumbo nucifera]
          Length = 485

 Score =  341 bits (875), Expect = 6e-91
 Identities = 169/358 (47%), Positives = 242/358 (67%), Gaps = 1/358 (0%)
 Frame = -1

Query: 1192 RTELFTPKMIESQAYIREDKALDMVKYLRXXXXXXXXXXXXVFGTLVNIFGNLIFSKDVF 1013
            RTELF+ KM++ QA+ RE+K  +++K+L             +FG+++NI G LIFSKDV+
Sbjct: 129  RTELFSTKMLDIQAHAREEKVSELMKFLIRKEGEKVNFADVIFGSILNILGALIFSKDVY 188

Query: 1012 ELGEGSRDGGSAEMKVLLWKLLEMGNSTNLADYFPVLGSLDLFGQRRAVAEVLKQIYDVW 833
            +  +  R   +  MK ++ +L+ +    NLAD +P+LG  D  G R+A A  +K++ + W
Sbjct: 189  DFED--RTDNNLGMKGMIRQLMILAAIPNLADLYPILGGSDFQGLRKASAACVKRMNESW 246

Query: 832  GAMLKERRGAKGDCRNDFVNVLIDSGLDDQKINALLMELFGAGTETSASTIEWAIAELTK 653
             A++K+RR      +NDF+ VL+DSG  D +I+A+L+E FG G++TS STIEWA+AEL +
Sbjct: 247  AAIVKQRRKNDDHSKNDFLQVLLDSGFSDPQIDAMLLETFGPGSDTSTSTIEWAMAELLR 306

Query: 652  NPRITSKLQSELQNIVGE-NTVKESDIPHLPYLQAFVKEXXXXXXXXXXXXXXXXLETCT 476
            NP    K++ EL  ++   N VKESD+P+LPYL A VKE                +ETC 
Sbjct: 307  NPEKLVKVREELDRVIRRSNNVKESDLPNLPYLHACVKETLRLHPPVTFLLPHRAMETCQ 366

Query: 475  VMNYTIPKDCQIMVNAWAIGRDPKTWDEPLRFKPERFLSCGVDFKGNDFELIPFGGGRRI 296
            +MNYTIPK CQ+MVN +AIGRD KTW++PL F PERFL+  +D++GNDF+ IPFG GRRI
Sbjct: 367  MMNYTIPKGCQLMVNTYAIGRDSKTWEKPLSFLPERFLNSELDYQGNDFQYIPFGAGRRI 426

Query: 295  CPGLPLASQFISLIVATFVQNFEWRLPDGVSSDELSMEEKFGLTLQKDPPLLIVTRAR 122
            CPGL LA++ + LI+A+ +  F+W LPDG+  DEL M +KFGL LQKD PL+++ + R
Sbjct: 427  CPGLSLATRVVRLILASLLHTFDWSLPDGMHPDELDMNDKFGLALQKDIPLVVIPKLR 484


>ref|XP_012076022.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
            [Jatropha curcas] gi|802796904|ref|XP_012092884.1|
            PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase
            isozyme 2 [Jatropha curcas]
          Length = 502

 Score =  328 bits (842), Expect = 4e-87
 Identities = 161/360 (44%), Positives = 231/360 (64%), Gaps = 3/360 (0%)
 Frame = -1

Query: 1192 RTELFTPKMIESQAYIREDKALDMVKYLRXXXXXXXXXXXXVFGTLVNIFGNLIFSKDVF 1013
            RTELF+ K IES+A +RE K  +M+++L             VF T+ N   NL+FSKD  
Sbjct: 146  RTELFSAKAIESKAALREQKVSEMIEFLGIKQGQVVNVGEVVFATVFNSISNLLFSKDFI 205

Query: 1012 ELGEGSRDGGSAEMKVLLWKLLEMGNSTNLADYFPVLGSLDLFGQRRAVAEVLKQIYDVW 833
               +     G   +K L+WKL+E+G++ NLAD++P+   LDL G R+ +++ LK+++D+W
Sbjct: 206  SFEDEESVSG---LKGLMWKLMELGSAPNLADFYPIFSGLDLQGLRKKMSKCLKEMFDIW 262

Query: 832  GAMLKERR---GAKGDCRNDFVNVLIDSGLDDQKINALLMELFGAGTETSASTIEWAIAE 662
               +KERR      G  + DF++V I +G DD++IN L+ ELF AGT+T+ +T+EWA+AE
Sbjct: 263  EIFIKERRERHARNGVVKEDFLDVFISNGFDDEQINWLIHELFSAGTDTTTTTVEWAMAE 322

Query: 661  LTKNPRITSKLQSELQNIVGENTVKESDIPHLPYLQAFVKEXXXXXXXXXXXXXXXXLET 482
            L KN    +K+  EL+  + +N++ ES +   PYL A +KE                 ET
Sbjct: 323  LLKNKEAMNKVCQELEREINKNSINESQVSQFPYLYACIKETFRLHPPAPFLGHQPL-ET 381

Query: 481  CTVMNYTIPKDCQIMVNAWAIGRDPKTWDEPLRFKPERFLSCGVDFKGNDFELIPFGGGR 302
            C VMNYTIPKD QI+VN WA+GRDP  W++PL FKPERFL   +D KG+DFEL+PF  GR
Sbjct: 382  CEVMNYTIPKDSQIIVNIWAVGRDPLAWEDPLLFKPERFLESSLDLKGHDFELLPFSSGR 441

Query: 301  RICPGLPLASQFISLIVATFVQNFEWRLPDGVSSDELSMEEKFGLTLQKDPPLLIVTRAR 122
            R+CPGLP+A +F+ LI+A+ +  F+W LP G     L M +K+G+TLQK+ PLL+V   +
Sbjct: 442  RMCPGLPMAIRFLPLILASLIHYFDWSLPGGEDPAMLDMNDKYGITLQKEKPLLLVPNTK 501


>gb|KDP20161.1| hypothetical protein JCGZ_00026 [Jatropha curcas]
            gi|643725464|gb|KDP34503.1| hypothetical protein
            JCGZ_11053 [Jatropha curcas]
          Length = 436

 Score =  328 bits (842), Expect = 4e-87
 Identities = 161/360 (44%), Positives = 231/360 (64%), Gaps = 3/360 (0%)
 Frame = -1

Query: 1192 RTELFTPKMIESQAYIREDKALDMVKYLRXXXXXXXXXXXXVFGTLVNIFGNLIFSKDVF 1013
            RTELF+ K IES+A +RE K  +M+++L             VF T+ N   NL+FSKD  
Sbjct: 80   RTELFSAKAIESKAALREQKVSEMIEFLGIKQGQVVNVGEVVFATVFNSISNLLFSKDFI 139

Query: 1012 ELGEGSRDGGSAEMKVLLWKLLEMGNSTNLADYFPVLGSLDLFGQRRAVAEVLKQIYDVW 833
               +     G   +K L+WKL+E+G++ NLAD++P+   LDL G R+ +++ LK+++D+W
Sbjct: 140  SFEDEESVSG---LKGLMWKLMELGSAPNLADFYPIFSGLDLQGLRKKMSKCLKEMFDIW 196

Query: 832  GAMLKERR---GAKGDCRNDFVNVLIDSGLDDQKINALLMELFGAGTETSASTIEWAIAE 662
               +KERR      G  + DF++V I +G DD++IN L+ ELF AGT+T+ +T+EWA+AE
Sbjct: 197  EIFIKERRERHARNGVVKEDFLDVFISNGFDDEQINWLIHELFSAGTDTTTTTVEWAMAE 256

Query: 661  LTKNPRITSKLQSELQNIVGENTVKESDIPHLPYLQAFVKEXXXXXXXXXXXXXXXXLET 482
            L KN    +K+  EL+  + +N++ ES +   PYL A +KE                 ET
Sbjct: 257  LLKNKEAMNKVCQELEREINKNSINESQVSQFPYLYACIKETFRLHPPAPFLGHQPL-ET 315

Query: 481  CTVMNYTIPKDCQIMVNAWAIGRDPKTWDEPLRFKPERFLSCGVDFKGNDFELIPFGGGR 302
            C VMNYTIPKD QI+VN WA+GRDP  W++PL FKPERFL   +D KG+DFEL+PF  GR
Sbjct: 316  CEVMNYTIPKDSQIIVNIWAVGRDPLAWEDPLLFKPERFLESSLDLKGHDFELLPFSSGR 375

Query: 301  RICPGLPLASQFISLIVATFVQNFEWRLPDGVSSDELSMEEKFGLTLQKDPPLLIVTRAR 122
            R+CPGLP+A +F+ LI+A+ +  F+W LP G     L M +K+G+TLQK+ PLL+V   +
Sbjct: 376  RMCPGLPMAIRFLPLILASLIHYFDWSLPGGEDPAMLDMNDKYGITLQKEKPLLLVPNTK 435


>ref|XP_002283415.2| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
            [Vitis vinifera]
          Length = 540

 Score =  326 bits (835), Expect = 3e-86
 Identities = 165/359 (45%), Positives = 231/359 (64%), Gaps = 2/359 (0%)
 Frame = -1

Query: 1192 RTELFTPKMIESQAYIREDKALDMVKYLRXXXXXXXXXXXXVFGTLVNIFGNLIFSKDVF 1013
            R ELF+ K++ESQA++ E K ++MV+++             VF T++N    ++ S+D  
Sbjct: 184  RAELFSTKVMESQAWVGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFI 243

Query: 1012 ELGEGSRDGGSAEMKVLLWKLLEMGNSTNLADYFPVLGSLDLFGQRRAVAEVLKQIYDVW 833
               + ++DGG   MK L+ K++    + NL D++ +   LDL G  +   E++ +I  +W
Sbjct: 244  SFEDDNKDGG---MKGLVRKMVMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARICSMW 300

Query: 832  GAMLKERR-GAKGD-CRNDFVNVLIDSGLDDQKINALLMELFGAGTETSASTIEWAIAEL 659
             ++++ERR GA  D  + DF+N+LI SG  D +IN L MEL  AG +TS+ST+EWA+AEL
Sbjct: 301  ESVIRERREGASDDPSKQDFLNILIRSGYSDDQINQLFMELLTAGADTSSSTLEWAMAEL 360

Query: 658  TKNPRITSKLQSELQNIVGENTVKESDIPHLPYLQAFVKEXXXXXXXXXXXXXXXXLETC 479
             K+P    K+  EL   + +N  K SD+PHLPYLQA VKE                  +C
Sbjct: 361  IKSPESMKKVHEELAREISDNLPKASDLPHLPYLQACVKETLRLHPSAPLLLPRRASVSC 420

Query: 478  TVMNYTIPKDCQIMVNAWAIGRDPKTWDEPLRFKPERFLSCGVDFKGNDFELIPFGGGRR 299
             VMNYTIPKD QI VNAWAIGRDP  W++PL FKPERFL+  VDFKGN+ E IPFG GRR
Sbjct: 421  EVMNYTIPKDSQIWVNAWAIGRDPMNWEDPLVFKPERFLNSAVDFKGNNLEFIPFGAGRR 480

Query: 298  ICPGLPLASQFISLIVATFVQNFEWRLPDGVSSDELSMEEKFGLTLQKDPPLLIVTRAR 122
            ICPGLP+A++ + LI+A+    F+W LP+G + DEL M +KFG+TLQK+ PLLI+ + R
Sbjct: 481  ICPGLPMAARLLPLILASLTHFFDWSLPNGTTPDELDMNDKFGVTLQKEQPLLIIPKVR 539


>emb|CBI34715.3| unnamed protein product [Vitis vinifera]
          Length = 1076

 Score =  325 bits (834), Expect = 4e-86
 Identities = 165/359 (45%), Positives = 231/359 (64%), Gaps = 2/359 (0%)
 Frame = -1

Query: 1192 RTELFTPKMIESQAYIREDKALDMVKYLRXXXXXXXXXXXXVFGTLVNIFGNLIFSKDVF 1013
            R ELF+ K++ESQA++ E K ++MV+++             VF T++N    ++ S+D  
Sbjct: 711  RAELFSTKVMESQAWVGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFI 770

Query: 1012 ELGEGSRDGGSAEMKVLLWKLLEMGNSTNLADYFPVLGSLDLFGQRRAVAEVLKQIYDVW 833
               + ++DGG   MK L+ K++    + NL D++ +   LDL G  +   E++ +I  +W
Sbjct: 771  SFEDDNKDGG---MKGLVRKMVMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARICSMW 827

Query: 832  GAMLKERR-GAKGD-CRNDFVNVLIDSGLDDQKINALLMELFGAGTETSASTIEWAIAEL 659
             ++++ERR GA  D  + DF+N+LI SG  D +IN L MEL  AG +TS+ST+EWA+AEL
Sbjct: 828  ESVIRERREGASDDPSKQDFLNILIRSGYSDDQINQLFMELLTAGADTSSSTLEWAMAEL 887

Query: 658  TKNPRITSKLQSELQNIVGENTVKESDIPHLPYLQAFVKEXXXXXXXXXXXXXXXXLETC 479
             K+P    K+  EL   + +N  K SD+PHLPYLQA VKE                  +C
Sbjct: 888  IKSPESMKKVHEELAREISDNLPKASDLPHLPYLQACVKETLRLHPSAPLLLPRRASVSC 947

Query: 478  TVMNYTIPKDCQIMVNAWAIGRDPKTWDEPLRFKPERFLSCGVDFKGNDFELIPFGGGRR 299
             VMNYTIPKD QI VNAWAIGRDP  W++PL FKPERFL+  VDFKGN+ E IPFG GRR
Sbjct: 948  EVMNYTIPKDSQIWVNAWAIGRDPMNWEDPLVFKPERFLNSAVDFKGNNLEFIPFGAGRR 1007

Query: 298  ICPGLPLASQFISLIVATFVQNFEWRLPDGVSSDELSMEEKFGLTLQKDPPLLIVTRAR 122
            ICPGLP+A++ + LI+A+    F+W LP+G + DEL M +KFG+TLQK+ PLLI+ + R
Sbjct: 1008 ICPGLPMAARLLPLILASLTHFFDWSLPNGTTPDELDMNDKFGVTLQKEQPLLIIPKKR 1066


>ref|XP_002510830.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
            communis] gi|223549945|gb|EEF51432.1|
            (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
            [Ricinus communis]
          Length = 496

 Score =  324 bits (831), Expect = 8e-86
 Identities = 161/361 (44%), Positives = 233/361 (64%), Gaps = 4/361 (1%)
 Frame = -1

Query: 1192 RTELFTPKMIESQAYIREDKALDMVKYLRXXXXXXXXXXXXVFGTLVNIFGNLIFSKDVF 1013
            RTELF+PK IESQA +RE K  +M+++L             VFGT+ N   +L+FS D+ 
Sbjct: 139  RTELFSPKAIESQAILREKKLAEMLEFLITKQGQAVNVAEVVFGTIFNTISHLLFSTDL- 197

Query: 1012 ELGEGSRDGGSAEMKVLLWKLLEMGNSTNLADYFPVLGSLDLFGQRRAVAEVLKQIYDVW 833
             +G  ++ GG   +K LLW ++EM  S N+A+++P+L  LD  G +R + + LK+++ VW
Sbjct: 198  -IGFENQIGG---VKSLLWSMMEMATSPNIAEFYPILAPLDPQGLKRKMTKCLKEMFGVW 253

Query: 832  GAMLKERRGAKGDC----RNDFVNVLIDSGLDDQKINALLMELFGAGTETSASTIEWAIA 665
               +KERR          + DF+++ + +G DD +IN L+MEL  AGT+T+++++EWA+A
Sbjct: 254  EIYIKERRRTHDHDHAAPKTDFLDIFLSNGFDDDQINWLVMELLSAGTDTTSTSVEWAMA 313

Query: 664  ELTKNPRITSKLQSELQNIVGENTVKESDIPHLPYLQAFVKEXXXXXXXXXXXXXXXXLE 485
            EL KN     K++ EL   + +N +KES +  LPYL A VKE                 E
Sbjct: 314  ELLKNKEAMKKVREELDREINKNPIKESHVSQLPYLNACVKETLRLHPPAPFLIPRRATE 373

Query: 484  TCTVMNYTIPKDCQIMVNAWAIGRDPKTWDEPLRFKPERFLSCGVDFKGNDFELIPFGGG 305
             C VMNYTIPKD Q++VN WAIGRDP  W++P  FKPERFL   +D KG+DFELIPFG G
Sbjct: 374  NCEVMNYTIPKDSQVLVNVWAIGRDPSVWEDPSSFKPERFLGSSLDVKGHDFELIPFGSG 433

Query: 304  RRICPGLPLASQFISLIVATFVQNFEWRLPDGVSSDELSMEEKFGLTLQKDPPLLIVTRA 125
            RRICPGLP+A++ +SL++A+ +  F+W LP+G    ++ M EKFG+TLQ + PLLI+ + 
Sbjct: 434  RRICPGLPMATRQLSLVLASLIHCFDWSLPNGGDPAKVDMTEKFGITLQMEHPLLIIPKP 493

Query: 124  R 122
            +
Sbjct: 494  K 494


>ref|XP_002283461.2| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
            [Vitis vinifera]
          Length = 540

 Score =  323 bits (829), Expect = 1e-85
 Identities = 164/359 (45%), Positives = 229/359 (63%), Gaps = 2/359 (0%)
 Frame = -1

Query: 1192 RTELFTPKMIESQAYIREDKALDMVKYLRXXXXXXXXXXXXVFGTLVNIFGNLIFSKDVF 1013
            R ELF+ K++ESQA++ E K ++MV+++             VF T++N    ++ S+D  
Sbjct: 184  RAELFSTKVMESQAWVGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFI 243

Query: 1012 ELGEGSRDGGSAEMKVLLWKLLEMGNSTNLADYFPVLGSLDLFGQRRAVAEVLKQIYDVW 833
               +  +DGG   MK L+ K++    + NL D++ +   LDL G  +   E++ +I  +W
Sbjct: 244  SFEDDYKDGG---MKGLVRKMMMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARICSMW 300

Query: 832  GAMLKERR-GAKGD-CRNDFVNVLIDSGLDDQKINALLMELFGAGTETSASTIEWAIAEL 659
             ++++ERR GA  D  + DF+N+LI SG  D +IN L MEL  AG +TS+ST+EWA+AEL
Sbjct: 301  ESVIRERREGASDDPSKQDFLNILIRSGYSDDQINQLFMELLTAGADTSSSTLEWAMAEL 360

Query: 658  TKNPRITSKLQSELQNIVGENTVKESDIPHLPYLQAFVKEXXXXXXXXXXXXXXXXLETC 479
             K+P    K+  EL   + +N  K SD+PHLPYLQA VKE                  +C
Sbjct: 361  IKSPESMKKVHEELAREISDNLPKASDLPHLPYLQACVKETLRLHPSAPLLLPRRASVSC 420

Query: 478  TVMNYTIPKDCQIMVNAWAIGRDPKTWDEPLRFKPERFLSCGVDFKGNDFELIPFGGGRR 299
             VMNYTIPKD QI VNAWAIGRDP  W++PL FKPERFL+  VDFKGN+ E IPFG GRR
Sbjct: 421  EVMNYTIPKDSQIWVNAWAIGRDPMNWEDPLVFKPERFLNSAVDFKGNNLEFIPFGAGRR 480

Query: 298  ICPGLPLASQFISLIVATFVQNFEWRLPDGVSSDELSMEEKFGLTLQKDPPLLIVTRAR 122
            ICPGLP+A++ + LI+A+    F+W LP+G + DEL M +KFG+ LQK+ PLLI+ + R
Sbjct: 481  ICPGLPMAARLLPLILASLTHFFDWSLPNGTTPDELDMNDKFGIALQKEQPLLIIPKVR 539


>emb|CBI34714.3| unnamed protein product [Vitis vinifera]
          Length = 936

 Score =  323 bits (829), Expect = 1e-85
 Identities = 164/359 (45%), Positives = 229/359 (63%), Gaps = 2/359 (0%)
 Frame = -1

Query: 1192 RTELFTPKMIESQAYIREDKALDMVKYLRXXXXXXXXXXXXVFGTLVNIFGNLIFSKDVF 1013
            R ELF+ K++ESQA++ E K ++MV+++             VF T++N    ++ S+D  
Sbjct: 580  RAELFSTKVMESQAWVGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFI 639

Query: 1012 ELGEGSRDGGSAEMKVLLWKLLEMGNSTNLADYFPVLGSLDLFGQRRAVAEVLKQIYDVW 833
               +  +DGG   MK L+ K++    + NL D++ +   LDL G  +   E++ +I  +W
Sbjct: 640  SFEDDYKDGG---MKGLVRKMMMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARICSMW 696

Query: 832  GAMLKERR-GAKGD-CRNDFVNVLIDSGLDDQKINALLMELFGAGTETSASTIEWAIAEL 659
             ++++ERR GA  D  + DF+N+LI SG  D +IN L MEL  AG +TS+ST+EWA+AEL
Sbjct: 697  ESVIRERREGASDDPSKQDFLNILIRSGYSDDQINQLFMELLTAGADTSSSTLEWAMAEL 756

Query: 658  TKNPRITSKLQSELQNIVGENTVKESDIPHLPYLQAFVKEXXXXXXXXXXXXXXXXLETC 479
             K+P    K+  EL   + +N  K SD+PHLPYLQA VKE                  +C
Sbjct: 757  IKSPESMKKVHEELAREISDNLPKASDLPHLPYLQACVKETLRLHPSAPLLLPRRASVSC 816

Query: 478  TVMNYTIPKDCQIMVNAWAIGRDPKTWDEPLRFKPERFLSCGVDFKGNDFELIPFGGGRR 299
             VMNYTIPKD QI VNAWAIGRDP  W++PL FKPERFL+  VDFKGN+ E IPFG GRR
Sbjct: 817  EVMNYTIPKDSQIWVNAWAIGRDPMNWEDPLVFKPERFLNSAVDFKGNNLEFIPFGAGRR 876

Query: 298  ICPGLPLASQFISLIVATFVQNFEWRLPDGVSSDELSMEEKFGLTLQKDPPLLIVTRAR 122
            ICPGLP+A++ + LI+A+    F+W LP+G + DEL M +KFG+ LQK+ PLLI+ + R
Sbjct: 877  ICPGLPMAARLLPLILASLTHFFDWSLPNGTTPDELDMNDKFGIALQKEQPLLIIPKVR 935


>ref|XP_011041604.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
            [Populus euphratica]
          Length = 492

 Score =  322 bits (826), Expect = 3e-85
 Identities = 158/359 (44%), Positives = 231/359 (64%), Gaps = 2/359 (0%)
 Frame = -1

Query: 1192 RTELFTPKMIESQAYIREDKALDMVKYLRXXXXXXXXXXXXVFGTLVNIFGNLIFSKDVF 1013
            R ELF+ K IESQA +RE K  +MV+++             V   + N   NL+FS D+ 
Sbjct: 136  RNELFSAKAIESQAVLREKKMGEMVEFIGRRKGEVVGIGEVVLAIVFNTIANLLFSVDLI 195

Query: 1012 ELGEGSRDGGSAEMKVLLWKLLEMGNSTNLADYFPVLGSLDLFGQRRAVAEVLKQIYDVW 833
             L +   DG +  +K L+W+++++G + N++D++P+LG +D  G +R +A  + Q++ +W
Sbjct: 196  GLED---DGAATGLKSLMWRMMKLGATPNISDFYPILGGIDPQGLKRKMAVCVNQMFGIW 252

Query: 832  GAMLKERRG--AKGDCRNDFVNVLIDSGLDDQKINALLMELFGAGTETSASTIEWAIAEL 659
            G  +KERR        R+DF++V + +G +D +IN L +EL  AGT+T+A+T+EWAIAEL
Sbjct: 253  GKYIKERREKHVHDGPRSDFLDVFLANGFEDLQINWLALELLSAGTDTTATTVEWAIAEL 312

Query: 658  TKNPRITSKLQSELQNIVGENTVKESDIPHLPYLQAFVKEXXXXXXXXXXXXXXXXLETC 479
             KN  +  K+  E++     N++KES +  LPYL A V E                LETC
Sbjct: 313  LKNKEVLKKVSEEIKRETDTNSLKESHVSQLPYLNACVNETLRLHPPVPFLIPRRALETC 372

Query: 478  TVMNYTIPKDCQIMVNAWAIGRDPKTWDEPLRFKPERFLSCGVDFKGNDFELIPFGGGRR 299
             VM+YTIP+D +++VN WA+GRDP  W++PL FKPERFL   +DFKG+DFE +PFG GRR
Sbjct: 373  KVMDYTIPRDSEVIVNVWAVGRDPSLWEDPLSFKPERFLGSDLDFKGHDFEFLPFGAGRR 432

Query: 298  ICPGLPLASQFISLIVATFVQNFEWRLPDGVSSDELSMEEKFGLTLQKDPPLLIVTRAR 122
            ICPGLP+A++ + LI+AT +  F+W LP+G     L M EKFG+TLQK+ PLL+V R R
Sbjct: 433  ICPGLPMAAKQVHLIIATLLYYFDWSLPNGEDPATLDMSEKFGITLQKEQPLLLVPRRR 491


>gb|AJD20229.1| corytuberine synthase [Sinopodophyllum hexandrum]
          Length = 483

 Score =  322 bits (824), Expect = 5e-85
 Identities = 159/357 (44%), Positives = 230/357 (64%)
 Frame = -1

Query: 1192 RTELFTPKMIESQAYIREDKALDMVKYLRXXXXXXXXXXXXVFGTLVNIFGNLIFSKDVF 1013
            RTE+F+PKM++ Q ++RE K  ++V++L+            VFGTL+NI GN+IFSKDVF
Sbjct: 130  RTEIFSPKMLQVQEHVREQKVAELVEFLKGKEGQVVKISQLVFGTLLNILGNVIFSKDVF 189

Query: 1012 ELGEGSRDGGSAEMKVLLWKLLEMGNSTNLADYFPVLGSLDLFGQRRAVAEVLKQIYDVW 833
                   D    +M+ L+ ++L +G + NLA+++P+    DL G ++   +   ++  +W
Sbjct: 190  RYVGADGD----KMQTLIKEMLMIGAAPNLAEFWPIFEEFDLQGLKKRCNQRFSEVMKMW 245

Query: 832  GAMLKERRGAKGDCRNDFVNVLIDSGLDDQKINALLMELFGAGTETSASTIEWAIAELTK 653
               ++ER+  + +   D ++VL+ +  +D +INA+ +E FG G+ETS++T EWA+AEL K
Sbjct: 246  KGTVEERKVKRNEEPKDMLDVLLLNDFNDAQINAVFLETFGPGSETSSATSEWALAELIK 305

Query: 652  NPRITSKLQSELQNIVGENTVKESDIPHLPYLQAFVKEXXXXXXXXXXXXXXXXLETCTV 473
            NP I +K++ EL  +VG++TVKESD+PHLPYLQA +KE                +ETC V
Sbjct: 306  NPHILAKVRQELDQVVGKSTVKESDLPHLPYLQACIKETMRLHPAAPFLLPHRAVETCPV 365

Query: 472  MNYTIPKDCQIMVNAWAIGRDPKTWDEPLRFKPERFLSCGVDFKGNDFELIPFGGGRRIC 293
            M YTIPKD Q++VN +AIGRDPKTW EP  FKPERFL   VD+ G  FE IPFG GRR C
Sbjct: 366  MGYTIPKDTQVLVNIYAIGRDPKTWKEPTTFKPERFLESDVDYYGKHFEFIPFGAGRRQC 425

Query: 292  PGLPLASQFISLIVATFVQNFEWRLPDGVSSDELSMEEKFGLTLQKDPPLLIVTRAR 122
             G+PLA++ I LIV+  VQ  +W LPDG    +L M E   L+L  DP L ++ ++R
Sbjct: 426  VGMPLATRTIPLIVSNLVQTLDWSLPDGKLPQDLVMNESLSLSLALDPTLTVIPKSR 482