BLASTX nr result
ID: Aconitum23_contig00036310
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Aconitum23_contig00036310
(950 letters)
Database: ./nr
77,306,371 sequences; 28,104,191,420 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. diss... 452 e-124
gb|AJD20229.1| corytuberine synthase [Sinopodophyllum hexandrum] 394 e-107
sp|P47195.1|C80A1_BERST RecName: Full=Berbamunine synthase; AltN... 296 2e-77
ref|XP_010253990.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 278 6e-72
ref|XP_010253989.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 262 3e-67
sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcocla... 252 3e-64
gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase ... 251 6e-64
sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-... 251 8e-64
gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chi... 249 3e-63
sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-... 248 4e-63
ref|XP_009609252.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 243 2e-61
ref|XP_004234364.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 239 2e-60
ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym... 239 3e-60
ref|XP_006353352.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 238 4e-60
gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [P... 238 7e-60
ref|XP_009757123.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 237 9e-60
sp|Q9SP06.1|C80B3_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-... 236 1e-59
emb|CDP15158.1| unnamed protein product [Coffea canephora] 236 2e-59
emb|CBI31830.3| unnamed protein product [Vitis vinifera] 235 3e-59
gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [... 235 3e-59
>dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. dissecta]
Length = 486
Score = 452 bits (1163), Expect = e-124
Identities = 221/315 (70%), Positives = 261/315 (82%)
Frame = +3
Query: 6 IFAILVLLTLASKTKHKNLPPGPRAWPLIGSLPMLFTNTHVPLHVTLANLAAAHGPIMTL 185
+ ++++ L K K+KNLPPGP WPLIG+LP+LFTNT VPLH+TLAN+A HGPIM L
Sbjct: 10 LIPVILVFILLLKPKYKNLPPGPHPWPLIGNLPILFTNTEVPLHITLANMARTHGPIMIL 69
Query: 186 WLGTQPTVIVSAPESAAEVLKTHDRACSGRYIPMSFRLKHHMKYSLVWADCTDYWKLLRK 365
WLGTQPTV+ S E+A E+LKTHDR S R+I MSFRLKHH+KYSLVW+DCTDYWKLLRK
Sbjct: 70 WLGTQPTVMASTAEAAMEILKTHDRIFSARHIRMSFRLKHHIKYSLVWSDCTDYWKLLRK 129
Query: 366 IARTEIFSPRMLQTQSRVREEKVGELMDYLRSRQGQAVKLSTFVFGTLLNILGNAVCSKD 545
I RTEIFSP+MLQ QS VRE+KV EL+D+LRS++GQ VK+S FVFGTLLNILGN V SKD
Sbjct: 130 IVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVKISQFVFGTLLNILGNVVFSKD 189
Query: 546 VFVYSDDDHEGGKQGLQSMLQEMLMIGAELNIAEFYPILEGLDLQGLKRKSDDRFGRVMK 725
VFVY D+ +G G+Q++++EMLMIGAE N+AEFYP LE LDLQGLK+K D+RF RVMK
Sbjct: 190 VFVYGDETDKG---GIQNLIREMLMIGAEPNVAEFYPSLEELDLQGLKKKCDERFIRVMK 246
Query: 726 LWEGTVXXXXXXXXXXSKDMLDVLLANNFNDAQISALFWETFGPGSETSSTTIEWVMAEL 905
+WEGTV SKDMLDVLLAN+FNDAQI+ALF ETFGPGSETSS TIEWV+AEL
Sbjct: 247 MWEGTVKERKANRNEESKDMLDVLLANDFNDAQINALFLETFGPGSETSSATIEWVIAEL 306
Query: 906 IKNPDKTAKVRKELD 950
IK+P + AKVRKEL+
Sbjct: 307 IKSPKEMAKVRKELN 321
>gb|AJD20229.1| corytuberine synthase [Sinopodophyllum hexandrum]
Length = 483
Score = 394 bits (1013), Expect = e-107
Identities = 193/310 (62%), Positives = 240/310 (77%)
Frame = +3
Query: 21 VLLTLASKTKHKNLPPGPRAWPLIGSLPMLFTNTHVPLHVTLANLAAAHGPIMTLWLGTQ 200
+LL + K + KNLPPGP+ WP+IG++P LF++ + P+HV L N+A HGPIM LWLGTQ
Sbjct: 14 ILLYILFKPRSKNLPPGPKPWPIIGNIPALFSSAY-PMHVALTNMARTHGPIMILWLGTQ 72
Query: 201 PTVIVSAPESAAEVLKTHDRACSGRYIPMSFRLKHHMKYSLVWADCTDYWKLLRKIARTE 380
PT + S E+A E+LKTHDR+ SGR + MSFRLKHH+KYSLVWADC++YWKLLRKI RTE
Sbjct: 73 PTAVASTTEAAMEILKTHDRSFSGRAVRMSFRLKHHIKYSLVWADCSEYWKLLRKIVRTE 132
Query: 381 IFSPRMLQTQSRVREEKVGELMDYLRSRQGQAVKLSTFVFGTLLNILGNAVCSKDVFVYS 560
IFSP+MLQ Q VRE+KV EL+++L+ ++GQ VK+S VFGTLLNILGN + SKDVF Y
Sbjct: 133 IFSPKMLQVQEHVREQKVAELVEFLKGKEGQVVKISQLVFGTLLNILGNVIFSKDVFRYV 192
Query: 561 DDDHEGGKQGLQSMLQEMLMIGAELNIAEFYPILEGLDLQGLKRKSDDRFGRVMKLWEGT 740
D G K +Q++++EMLMIGA N+AEF+PI E DLQGLK++ + RF VMK+W+GT
Sbjct: 193 GAD--GDK--MQTLIKEMLMIGAAPNLAEFWPIFEEFDLQGLKKRCNQRFSEVMKMWKGT 248
Query: 741 VXXXXXXXXXXSKDMLDVLLANNFNDAQISALFWETFGPGSETSSTTIEWVMAELIKNPD 920
V KDMLDVLL N+FNDAQI+A+F ETFGPGSETSS T EW +AELIKNP
Sbjct: 249 VEERKVKRNEEPKDMLDVLLLNDFNDAQINAVFLETFGPGSETSSATSEWALAELIKNPH 308
Query: 921 KTAKVRKELD 950
AKVR+ELD
Sbjct: 309 ILAKVRQELD 318
>sp|P47195.1|C80A1_BERST RecName: Full=Berbamunine synthase; AltName:
Full=(S)-N-methylcoclaurine oxidase [C-O
phenol-coupling]; AltName: Full=CYPLXXX; AltName:
Full=Cytochrome P450 80 gi|642386|gb|AAC48987.1|
cytochrome P-450 CYP80 [Berberis stolonifera]
Length = 487
Score = 296 bits (757), Expect = 2e-77
Identities = 145/315 (46%), Positives = 206/315 (65%)
Frame = +3
Query: 6 IFAILVLLTLASKTKHKNLPPGPRAWPLIGSLPMLFTNTHVPLHVTLANLAAAHGPIMTL 185
I + +L L K KH NLPP P AWP++G LP L + P ++N+A +GP++ L
Sbjct: 10 ISLVALLYFLLFKPKHTNLPPSPPAWPIVGHLPDLISKNSPPFLDYMSNIAQKYGPLIHL 69
Query: 186 WLGTQPTVIVSAPESAAEVLKTHDRACSGRYIPMSFRLKHHMKYSLVWADCTDYWKLLRK 365
G ++ S E+A EVL+T+D+ SGR FR+K H+ YS++W+D YWK RK
Sbjct: 70 KFGLHSSIFASTKEAAMEVLQTNDKVLSGRQPLPCFRIKPHIDYSILWSDSNSYWKKGRK 129
Query: 366 IARTEIFSPRMLQTQSRVREEKVGELMDYLRSRQGQAVKLSTFVFGTLLNILGNAVCSKD 545
I TEIFS +MLQ Q + RE G L++++ ++ G V+L +++FG LN+LG+ V SKD
Sbjct: 130 ILHTEIFSQKMLQAQEKNRERVAGNLVNFIMTKVGDVVELRSWLFGCALNVLGHVVFSKD 189
Query: 546 VFVYSDDDHEGGKQGLQSMLQEMLMIGAELNIAEFYPILEGLDLQGLKRKSDDRFGRVMK 725
VF YSD E G+ ++ MLM G + ++A ++P+L DL GLKRK D++F ++K
Sbjct: 190 VFEYSDQSDE---VGMDKLIHGMLMTGGDFDVASYFPVLARFDLHGLKRKMDEQFKLLIK 246
Query: 726 LWEGTVXXXXXXXXXXSKDMLDVLLANNFNDAQISALFWETFGPGSETSSTTIEWVMAEL 905
+WEG V KDMLDVL+AN+FN+ QI+A+F ETFGPGS+T+S IEW +A+L
Sbjct: 247 IWEGEVLARRANRNPEPKDMLDVLIANDFNEHQINAMFMETFGPGSDTNSNIIEWALAQL 306
Query: 906 IKNPDKTAKVRKELD 950
IKNPDK AK+R+ELD
Sbjct: 307 IKNPDKLAKLREELD 321
>ref|XP_010253990.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nelumbo nucifera]
Length = 485
Score = 278 bits (710), Expect = 6e-72
Identities = 145/318 (45%), Positives = 205/318 (64%), Gaps = 3/318 (0%)
Frame = +3
Query: 6 IFAILVLLT--LASKTKHKNLPPGPRAWPLIGSL-PMLFTNTHVPLHVTLANLAAAHGPI 176
IF +L +L+ L K NLPPGP +WP+IG+L P L HV L + LA GP+
Sbjct: 8 IFFLLSILSVVLFLKPSPNNLPPGPFSWPIIGTLLPKLKKQPHVEL----SKLAQRFGPL 63
Query: 177 MTLWLGTQPTVIVSAPESAAEVLKTHDRACSGRYIPMSFRLKHHMKYSLVWADCTDYWKL 356
M L G +P V+ S+ +A EVLK DR SGR+ P S R+K ++++S+VWADCTDYWK+
Sbjct: 64 MLLKFGVEPVVVASSHVAAVEVLKNQDRLLSGRFAPHSVRIKGYIEHSMVWADCTDYWKM 123
Query: 357 LRKIARTEIFSPRMLQTQSRVREEKVGELMDYLRSRQGQAVKLSTFVFGTLLNILGNAVC 536
+RK+ RTE+FS +ML Q+ REEKV ELM +L ++G+ V + +FG++LNILG +
Sbjct: 124 VRKVWRTELFSTKMLDIQAHAREEKVSELMKFLIRKEGEKVNFADVIFGSILNILGALIF 183
Query: 537 SKDVFVYSDDDHEGGKQGLQSMLQEMLMIGAELNIAEFYPILEGLDLQGLKRKSDDRFGR 716
SKD VY +D G++ M+++++++ A N+A+ YPIL G D QGL++ S R
Sbjct: 184 SKD--VYDFEDRTDNNLGMKGMIRQLMILAAIPNLADLYPILGGSDFQGLRKASAACVKR 241
Query: 717 VMKLWEGTVXXXXXXXXXXSKDMLDVLLANNFNDAQISALFWETFGPGSETSSTTIEWVM 896
+ + W V D L VLL + F+D QI A+ ETFGPGS+TS++TIEW M
Sbjct: 242 MNESWAAIVKQRRKNDDHSKNDFLQVLLDSGFSDPQIDAMLLETFGPGSDTSTSTIEWAM 301
Query: 897 AELIKNPDKTAKVRKELD 950
AEL++NP+K KVR+ELD
Sbjct: 302 AELLRNPEKLVKVREELD 319
>ref|XP_010253989.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nelumbo nucifera]
Length = 486
Score = 262 bits (669), Expect = 3e-67
Identities = 139/318 (43%), Positives = 203/318 (63%), Gaps = 2/318 (0%)
Frame = +3
Query: 3 IIFAILVL-LTLASKTKHKNLPPGPRAWPLIGSLPMLFTNTHVPLHVTLANLAAAHGPIM 179
I+F + +L L L K K LPPGP +WP+IG+ L + T P ++ L LA +GP+M
Sbjct: 8 ILFLLSILSLVLFLKPSSKKLPPGPFSWPIIGT--QLPSPTMKP-NLELFKLAQRYGPLM 64
Query: 180 TLWLGTQPTVIVSAPESAAEVLKTHDRACSGRYIPMSFRLKHHMKYSLVWADCTDYWKLL 359
G + V+ S +A EVLK DR SGR+ S R+K +++YS+VWADCTDYWK++
Sbjct: 65 LFRFGFENVVVASNHVAAMEVLKNQDRVLSGRFKANSVRVKGYIEYSMVWADCTDYWKMV 124
Query: 360 RKIARTEIFSPRMLQTQSRVREEKVGELMDYLRSRQGQAVKLSTFVFGTLLNILGNAVCS 539
RKI RTE+FS +ML + VREEKV ELM +LR ++G+ V +FG +LN+LG + S
Sbjct: 125 RKILRTELFSTKMLDVHAHVREEKVSELMKFLRRKEGEEVNFVDVIFGCILNMLGALIYS 184
Query: 540 KDVFVYSDDDHEGGKQGLQSMLQEMLMIGAELNIAEFYPI-LEGLDLQGLKRKSDDRFGR 716
KD VY +D G++ M+++++++ A NIA+ YPI +G D QGL+++S R
Sbjct: 185 KD--VYDFEDKTDINLGMKGMIRQLMILAATPNIADLYPIFFDGSDFQGLRKESAACVKR 242
Query: 717 VMKLWEGTVXXXXXXXXXXSKDMLDVLLANNFNDAQISALFWETFGPGSETSSTTIEWVM 896
+ + W + D+L VLL + F+D QI A+F ETFGPGS+TS++TIEW +
Sbjct: 243 MSESWAAIINERRKNNDHTKNDLLQVLLDSGFSDPQIDAIFLETFGPGSDTSASTIEWAL 302
Query: 897 AELIKNPDKTAKVRKELD 950
AEL++NP+K K+ +ELD
Sbjct: 303 AELLRNPEKLVKLHEELD 320
>sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase
isozyme 2; AltName: Full=Cytochrome P450 80B2 [Coptis
japonica] gi|9971208|dbj|BAB12433.1|
(S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
Length = 488
Score = 252 bits (644), Expect = 3e-64
Identities = 132/312 (42%), Positives = 197/312 (63%), Gaps = 1/312 (0%)
Frame = +3
Query: 15 ILVLLTLASKTKHKNLPPGPRAWPLIGSLPMLFTNTHVPLHVTLANLAAAHGPIMTLWLG 194
+L L + ++ KNLPPGPR P++G+L L H A LA +G + +L LG
Sbjct: 14 LLFLFFILRDSRPKNLPPGPRPSPIVGNLLQLGDKPHAEF----AKLAQKYGELFSLKLG 69
Query: 195 TQPTVIVSAPESAAEVLKTHDRACSGRYIPMSFRLKHHMKYSLVWADCTDYWKLLRKIAR 374
+Q V+ S+P +AAE+LKTHD+ SGRY+ SFR+K H++ S+VW++C D WKLLRK+ R
Sbjct: 70 SQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDNWKLLRKVCR 129
Query: 375 TEIFSPRMLQTQSRVREEKVGELMDYLRSRQGQAVKLSTFVFGTLLNILGNAVCSKDVFV 554
TE+F+P+M+++QS +RE K E++ +LR ++G+ VK+ VFGTL+NI GN + SKDVF
Sbjct: 130 TELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVVFGTLVNIFGNLIFSKDVF- 188
Query: 555 YSDDDHEGGKQGLQSMLQEMLMIGAELNIAEFYPILEGLDLQGLKRKSDDRFGRVMKLWE 734
+D GG L+ L ++L +G N A+++PI+ LDL G +R + ++ +W
Sbjct: 189 -DLEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVLQQIYDVWG 247
Query: 735 GTVXXXXXXXXXXSK-DMLDVLLANNFNDAQISALFWETFGPGSETSSTTIEWVMAELIK 911
+ SK D +DVLL +D +I+AL E FG G+ETS++TIEW + EL K
Sbjct: 248 VMLKERRGTKGSESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSASTIEWAITELTK 307
Query: 912 NPDKTAKVRKEL 947
P +K+R EL
Sbjct: 308 KPLVVSKIRLEL 319
>gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase from Eschscholzia
californica [Expression vector pWCD2353]
Length = 490
Score = 251 bits (641), Expect = 6e-64
Identities = 128/313 (40%), Positives = 190/313 (60%), Gaps = 4/313 (1%)
Frame = +3
Query: 21 VLLTLASKTKHKNLPPGPRAWPLIGSLPMLFTNTHVPLHVTLANLAAAHGPIMTLWLGTQ 200
+L L + HKNLPPGP+ WP++G+L L H A LA +G I TL +GT+
Sbjct: 16 ILYLLFGSSGHKNLPPGPKPWPIVGNLLQLGEKPHAQF----AELAQTYGDIFTLKMGTE 71
Query: 201 PTVIVSAPESAAEVLKTHDRACSGRYIPMSFRLKHHMKYSLVWADCTDYWKLLRKIARTE 380
V+ S +A+E+LKTHDR S RY+ SFR+K H++ S+VW+DCT+ WK LRK+ RTE
Sbjct: 72 TVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWKNLRKVCRTE 131
Query: 381 IFSPRMLQTQSRVREEKVGELMDYLRSRQGQAVKLSTFVFGTLLNILGNAVCSKDVFVYS 560
+F+ +M+++Q+ VRE+K E+++YL +QG+ VK+ +FGTL+NI GN + S+++F
Sbjct: 132 LFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQNIFELG 191
Query: 561 DDDHEGGKQGLQSMLQEMLMIGAELNIAEFYPILEGLDLQGLKRKSDDRFGRVMKLWEGT 740
D + G + L ML +G N A+++P+L DL G +++ + + +W
Sbjct: 192 DPN--SGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAIWGAM 249
Query: 741 V----XXXXXXXXXXSKDMLDVLLANNFNDAQISALFWETFGPGSETSSTTIEWVMAELI 908
+ D +DV L + ND QI+AL E FG G+ETS++TIEW M EL
Sbjct: 250 LQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAMTELT 309
Query: 909 KNPDKTAKVRKEL 947
KNP TAK+R EL
Sbjct: 310 KNPKITAKLRSEL 322
>sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
AltName: Full=Cytochrome P450 80B1
gi|3127027|gb|AAC39452.1| (S)-N-methylcoclaurine
3'-hydroxylase [Eschscholzia californica]
Length = 487
Score = 251 bits (640), Expect = 8e-64
Identities = 132/319 (41%), Positives = 194/319 (60%), Gaps = 4/319 (1%)
Frame = +3
Query: 3 IIFAILVLLTLASKTKHKNLPPGPRAWPLIGSLPMLFTNTHVPLHVTLANLAAAHGPIMT 182
II +IL LL S HKNLPPGP+ WP++G+L L H A LA +G I T
Sbjct: 11 IISSILYLLFGGSG--HKNLPPGPKPWPIVGNLLQLGEKPHAQF----AELAQTYGDIFT 64
Query: 183 LWLGTQPTVIVSAPESAAEVLKTHDRACSGRYIPMSFRLKHHMKYSLVWADCTDYWKLLR 362
L +GT+ V+ S +A+E+LKTHDR S RY+ SFR+K H++ S+VW+DCT+ WK LR
Sbjct: 65 LKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWKNLR 124
Query: 363 KIARTEIFSPRMLQTQSRVREEKVGELMDYLRSRQGQAVKLSTFVFGTLLNILGNAVCSK 542
K+ RTE+F+ +M+++Q+ VRE+K E+++YL +QG+ VK+ +FGTL+NI GN + S+
Sbjct: 125 KVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQ 184
Query: 543 DVFVYSDDDHEGGKQGLQSMLQEMLMIGAELNIAEFYPILEGLDLQGLKRKSDDRFGRVM 722
++F D + G + L ML +G N A+++P+L DL G +++ + +
Sbjct: 185 NIFELGDPN--SGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIY 242
Query: 723 KLWEGTV----XXXXXXXXXXSKDMLDVLLANNFNDAQISALFWETFGPGSETSSTTIEW 890
+W + D +DV L + ND QI+AL E FG G+ETS++TIEW
Sbjct: 243 AIWGAMLQERKLAKKVDGYKSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEW 302
Query: 891 VMAELIKNPDKTAKVRKEL 947
M EL KNP TAK+R E+
Sbjct: 303 AMTELTKNPKITAKIRSEI 321
>gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
Length = 488
Score = 249 bits (635), Expect = 3e-63
Identities = 132/312 (42%), Positives = 193/312 (61%), Gaps = 1/312 (0%)
Frame = +3
Query: 15 ILVLLTLASKTKHKNLPPGPRAWPLIGSLPMLFTNTHVPLHVTLANLAAAHGPIMTLWLG 194
+L L + + KNLPPGPR P++G+L L A LA +G + +L LG
Sbjct: 14 LLFLFFILRDPRQKNLPPGPRPLPIVGNLLQLGDKPRAEF----AKLAQKYGQLFSLKLG 69
Query: 195 TQPTVIVSAPESAAEVLKTHDRACSGRYIPMSFRLKHHMKYSLVWADCTDYWKLLRKIAR 374
+Q V+ S+P +AAEVLKTHD+ SGRY+ SFR+K H++ S+VW++C D WKLLRK+ R
Sbjct: 70 SQTVVVASSPAAAAEVLKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDNWKLLRKVCR 129
Query: 375 TEIFSPRMLQTQSRVREEKVGELMDYLRSRQGQAVKLSTFVFGTLLNILGNAVCSKDVFV 554
TE+F+P+M+++QS +RE K E++ +LRS++G+ VK+ VFGTL+NI GN + SKDVF
Sbjct: 130 TELFTPKMIESQSEIREGKAREMVKFLRSKEGEVVKIVEVVFGTLVNIFGNLIFSKDVF- 188
Query: 555 YSDDDHEGGKQGLQSMLQEMLMIGAELNIAEFYPILEGLDLQGLKRKSDDRFGRVMKLWE 734
+D GG L+ L ++L +G N A+++PI+ DL G +R D ++ +W
Sbjct: 189 -DLEDPTGGSVELKEHLWKLLEMGNSTNPADYFPIMGKYDLFGQRRAVADVLQQIYDVWG 247
Query: 735 GTVXXXXXXXXXXS-KDMLDVLLANNFNDAQISALFWETFGPGSETSSTTIEWVMAELIK 911
+ S D +DVLL D +I+AL E FG G+ETS++TIEW + EL K
Sbjct: 248 INLKERRGMKGSESNNDFVDVLLNAGLEDQKINALLMELFGTGTETSASTIEWAITELTK 307
Query: 912 NPDKTAKVRKEL 947
P +K+R EL
Sbjct: 308 KPLVVSKIRLEL 319
>sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2;
AltName: Full=Cytochrome P450 80B2
gi|3127029|gb|AAC39453.1| (S)-N-methylcoclaurine
3'-hydroxylase [Eschscholzia californica]
Length = 488
Score = 248 bits (634), Expect = 4e-63
Identities = 127/313 (40%), Positives = 189/313 (60%), Gaps = 4/313 (1%)
Frame = +3
Query: 21 VLLTLASKTKHKNLPPGPRAWPLIGSLPMLFTNTHVPLHVTLANLAAAHGPIMTLWLGTQ 200
+L L + HKNLPPGP+ WP++G+L L H A LA +G I TL +GT+
Sbjct: 16 ILYLLFGSSGHKNLPPGPKPWPIVGNLLQLGEKPHAQF----AELAQTYGDIFTLKMGTE 71
Query: 201 PTVIVSAPESAAEVLKTHDRACSGRYIPMSFRLKHHMKYSLVWADCTDYWKLLRKIARTE 380
V+ S +A+E+LKTHDR S RY+ SFR+K H++ S+VW+DCT+ WK LRK+ RTE
Sbjct: 72 TVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWKNLRKVCRTE 131
Query: 381 IFSPRMLQTQSRVREEKVGELMDYLRSRQGQAVKLSTFVFGTLLNILGNAVCSKDVFVYS 560
+F+ +M+++Q+ VRE+K E+++YL +QG+ VK+ +FGTL+NI GN + S+++F
Sbjct: 132 LFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQNIFELG 191
Query: 561 DDDHEGGKQGLQSMLQEMLMIGAELNIAEFYPILEGLDLQGLKRKSDDRFGRVMKLWEGT 740
+ G + L ML +G N A+++P+L DL G +++ + + +W
Sbjct: 192 XPN--SGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECLKGIYAIWGAM 249
Query: 741 V----XXXXXXXXXXSKDMLDVLLANNFNDAQISALFWETFGPGSETSSTTIEWVMAELI 908
+ D +DV L + ND QI+AL E FG G+ETS++TIEW M EL
Sbjct: 250 LQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSASTIEWAMTELT 309
Query: 909 KNPDKTAKVRKEL 947
KNP TAK+R EL
Sbjct: 310 KNPKITAKLRSEL 322
>ref|XP_009609252.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nicotiana tomentosiformis]
Length = 495
Score = 243 bits (620), Expect = 2e-61
Identities = 134/319 (42%), Positives = 201/319 (63%), Gaps = 7/319 (2%)
Frame = +3
Query: 15 ILVLLTLASKTKHKNLPPGPRAWPLIGSLPMLFTNTHVPLHVTLANLAAAHGPIMTLWLG 194
I LL L +K LPPGP+ WP++G++ M +P H+TL N A +GP+M+L LG
Sbjct: 19 IFFLLKLCKSSKIPQLPPGPKPWPILGNIHM----GKMP-HITLTNFAKTYGPLMSLKLG 73
Query: 195 TQPTVIVSAPESAAEVLKTHDRACSGRYIPMSFRLKHHM--KYSLVW-ADCTDYWKLLRK 365
TQ V+ S+P A E+LKTHDR SGR++P +F K K S+ W ++C + W+ LR
Sbjct: 74 TQCLVVGSSPSVAIEILKTHDRILSGRHVPNAFPFKKSELEKSSIGWTSECNNGWRYLRT 133
Query: 366 IARTEIFSPRMLQTQSRVREEKVGELMDYLRSRQGQAVKLSTFVFGTLLNILGNAVCSKD 545
+ RTE+FS ++L++Q+ +RE+KV EL+++LRS++GQ V L +F T+LN+L N + S+D
Sbjct: 134 LCRTELFSGKVLESQACLREKKVKELIEFLRSKEGQVVNLGELIFATVLNMLSNVMISQD 193
Query: 546 VFVYSDDDHEGGKQGLQSMLQEMLMIGAELNIAEFYPILEGLDLQGLKRKSDDRFGRV-M 722
+ + +G G++S+++E + +G+ NI++FYPIL LDLQGL+RKS ++
Sbjct: 194 IVKLEKETEDG---GMKSLIREAMEVGSTPNISDFYPILCKLDLQGLRRKSMVLMSKIRS 250
Query: 723 KLWEGTVXXXXXXXXXXSK---DMLDVLLANNFNDAQISALFWETFGPGSETSSTTIEWV 893
K WE V S D L+ LL N F + I LF E F G++T+++TIEW
Sbjct: 251 KTWERIVEERRKSKESGSSSQHDFLETLLNNGFTNDAIHQLFMELFTAGTDTTTSTIEWA 310
Query: 894 MAELIKNPDKTAKVRKELD 950
+AELIKN + KVR EL+
Sbjct: 311 IAELIKNEESMEKVRAELE 329
>ref|XP_004234364.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Solanum lycopersicum]
Length = 486
Score = 239 bits (611), Expect = 2e-60
Identities = 127/318 (39%), Positives = 201/318 (63%), Gaps = 3/318 (0%)
Frame = +3
Query: 3 IIFAILVLLTLASKTKHKNLPPGPRAWPLIGSLPMLFTNTHVPLHVTLANLAAAHGPIMT 182
I F +L+ L K+ NLPPGPR W L+G+L + HV +LA A HGP+++
Sbjct: 14 IPFFLLIFKQLVPKST--NLPPGPRPWHLLGNLLQIGKTPHV----SLAKFAQIHGPLIS 67
Query: 183 LWLGTQPTVIVSAPESAAEVLKTHDRACSGRYIPM--SFRLKHHMKYSLVWA-DCTDYWK 353
+ LGTQ V+ S+P SAAE+LK+ DR S R +P ++ L ++S+V++ D ++ WK
Sbjct: 68 VKLGTQLVVVASSPASAAEILKSQDRLLSVRSVPKVATYELSVIDQHSIVFSSDLSNQWK 127
Query: 354 LLRKIARTEIFSPRMLQTQSRVREEKVGELMDYLRSRQGQAVKLSTFVFGTLLNILGNAV 533
LR RT +FSP+ +++Q+ +RE+KV E+M++LRSR+G+ VK+S +FGT+LN LGN
Sbjct: 128 FLRAFCRTHLFSPKAVESQAALREKKVSEMMNFLRSRKGETVKISEILFGTILNTLGNLF 187
Query: 534 CSKDVFVYSDDDHEGGKQGLQSMLQEMLMIGAELNIAEFYPILEGLDLQGLKRKSDDRFG 713
SKD+ D D+E G++ ++++ + +GA NI+EFYP+ + LDLQGL+++++
Sbjct: 188 FSKDL---CDLDYETNTSGIKHVIRKFVELGAMPNISEFYPLFDALDLQGLRKQTEIYQN 244
Query: 714 RVMKLWEGTVXXXXXXXXXXSKDMLDVLLANNFNDAQISALFWETFGPGSETSSTTIEWV 893
R++ +W V S D LD ++ N F+D Q+ L E GS+T+++TIEW
Sbjct: 245 RLVNIWSEIVKEKRQAISRSSSDFLDAMIDNGFSDLQVDILLMELISAGSDTTTSTIEWA 304
Query: 894 MAELIKNPDKTAKVRKEL 947
MAEL++N K++ EL
Sbjct: 305 MAELLRNKGAMRKLKAEL 322
>ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
communis] gi|223547255|gb|EEF48750.1|
(S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
[Ricinus communis]
Length = 496
Score = 239 bits (609), Expect = 3e-60
Identities = 128/321 (39%), Positives = 201/321 (62%), Gaps = 6/321 (1%)
Frame = +3
Query: 6 IFAILVLLTLAS-KTKHKNLPPGPRAWPLIGSLPMLFTNTHVPLHVTLANLAAAHGPIMT 182
+ I+V+ L+S +K + LPPGPR WP++G++ + N H+ ++AN A HGP+++
Sbjct: 18 LILIIVMQILSSISSKRRPLPPGPRQWPILGNILQVGKNPHI----SMANYAKVHGPLIS 73
Query: 183 LWLGTQPTVIVSAPESAAEVLKTHDRACSGRYIPMSFRLKHHM--KYSLVW-ADCTDYWK 353
L LGT+ V+ S+P +AAE+LKTHDR SGRYIP + + ++ + +LVW C+D WK
Sbjct: 74 LRLGTRVVVVASSPTAAAEILKTHDRLLSGRYIPATTPYEDNVLDRIALVWNPSCSDQWK 133
Query: 354 LLRKIARTEIFSPRMLQTQSRVREEKVGELMDYLRSRQGQAVKLSTFVFGTLLNILGNAV 533
LR + R+E+FS + +++Q+ +RE+K+ E++D+L S+QGQ V + VF T N + N +
Sbjct: 134 FLRAMCRSELFSAKAIESQATLREKKLTEMLDFLTSKQGQIVNIGEVVFTTAFNTISNLL 193
Query: 534 CSKDVFVYSDDDHEGGKQGLQSMLQEMLMIGAELNIAEFYPILEGLDLQGLKRKSDDRFG 713
SKD+ + D + G + L S L E+ NIA+FYP+L LD QG+KRK +
Sbjct: 194 FSKDLLSFEDQGNAGELKTLISTLMELATCP---NIADFYPVLTKLDPQGIKRKMKNCLE 250
Query: 714 RVMKLWEGTVXXXXXXXXXXSK--DMLDVLLANNFNDAQISALFWETFGPGSETSSTTIE 887
R+ +W+ + ++ D LDV L+N F+D QI+ L E F G++T++TT+E
Sbjct: 251 RMFGVWDIYIKERRERHVKDARKTDFLDVFLSNGFDDHQINWLLLELFSAGADTTTTTVE 310
Query: 888 WVMAELIKNPDKTAKVRKELD 950
W MAEL+K KVR+EL+
Sbjct: 311 WAMAELLKEITVLEKVREELE 331
>ref|XP_006353352.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Solanum tuberosum]
Length = 486
Score = 238 bits (608), Expect = 4e-60
Identities = 124/318 (38%), Positives = 204/318 (64%), Gaps = 3/318 (0%)
Frame = +3
Query: 3 IIFAILVLLTLASKTKHKNLPPGPRAWPLIGSLPMLFTNTHVPLHVTLANLAAAHGPIMT 182
I F +L+L L K+ NLPPGPR WPL+G++ + + H+ +LA A +GP+++
Sbjct: 14 IAFFLLILKQLIPKSP--NLPPGPRPWPLLGNILQIGKSPHI----SLAQFAKIYGPLIS 67
Query: 183 LWLGTQPTVIVSAPESAAEVLKTHDRACSGRYIPM--SFRLKHHMKYSLVWA-DCTDYWK 353
+ LGTQ V+ S+P+SAAE+LKT DR S R +P ++ L ++S+V++ D +++WK
Sbjct: 68 VKLGTQLVVVASSPDSAAEILKTQDRLLSVRSVPKVATYELSVIDQHSIVFSSDLSNHWK 127
Query: 354 LLRKIARTEIFSPRMLQTQSRVREEKVGELMDYLRSRQGQAVKLSTFVFGTLLNILGNAV 533
LR RT +FSP+ +++Q+ +RE+KV E++ +LRSR+G+ VK+S +F T+LN LGN
Sbjct: 128 FLRAFCRTHLFSPKAVESQAALREKKVSEMIHFLRSRKGETVKISEIMFATILNTLGNLF 187
Query: 534 CSKDVFVYSDDDHEGGKQGLQSMLQEMLMIGAELNIAEFYPILEGLDLQGLKRKSDDRFG 713
SKD+ D D+E G++ ++++ + +GA NI+EFYP+ + LDLQGL+++++
Sbjct: 188 FSKDL---CDLDYETNTSGIKHVIRKFVELGAMPNISEFYPLFDALDLQGLRKQTEIYQN 244
Query: 714 RVMKLWEGTVXXXXXXXXXXSKDMLDVLLANNFNDAQISALFWETFGPGSETSSTTIEWV 893
++ +W V S D LDV++ + F+D Q+ L E GS+T+++TIEW
Sbjct: 245 QLFNIWSDIVKEKRQAISRSSSDFLDVMIDSGFSDLQVDILLMELISAGSDTTTSTIEWA 304
Query: 894 MAELIKNPDKTAKVRKEL 947
MAEL++N K++ EL
Sbjct: 305 MAELLRNKGAMHKLQAEL 322
>gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [Papaver bracteatum]
Length = 486
Score = 238 bits (606), Expect = 7e-60
Identities = 118/301 (39%), Positives = 184/301 (61%), Gaps = 3/301 (0%)
Frame = +3
Query: 54 KNLPPGPRAWPLIGSLPMLFTNTHVPLHVTLANLAAAHGPIMTLWLGTQPTVIVSAPESA 233
K LPPGP+ WP++G+L L H A LA +G + TL LG++ V+ S P +A
Sbjct: 26 KGLPPGPKPWPIVGNLLQLGEKPHSQF----AELAKTYGDLFTLKLGSETVVVASTPLAA 81
Query: 234 AEVLKTHDRACSGRYIPMSFRLKHHMKYSLVWADCTDYWKLLRKIARTEIFSPRMLQTQS 413
+E+LKTHDR SGRY+ SFR+K H++ S+VW++C + WK LRK+ R E+F+ +M+++Q+
Sbjct: 82 SEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRAELFTQKMIESQA 141
Query: 414 RVREEKVGELMDYLRSRQGQAVKLSTFVFGTLLNILGNAVCSKDVFVYSDDDHEGGKQGL 593
+RE K E++++L+ QG VK+ VFGTL+NI GN + S+++F D+ G +
Sbjct: 142 EIRESKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFSQNIFKLGDE--SSGSVEM 199
Query: 594 QSMLQEMLMIGAELNIAEFYPILEGLDLQGLKRKSDDRFGRVMKLWEGTVXXXXXXXXXX 773
+ L ML +G N A+++P L DL G ++ D + +W +
Sbjct: 200 KEHLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGIYSVWGAMLKERKIAKLHN 259
Query: 774 S---KDMLDVLLANNFNDAQISALFWETFGPGSETSSTTIEWVMAELIKNPDKTAKVRKE 944
+ D +++LL + +D QI+AL E FG G+ETS++TIEW ++EL KNP+ TA +R E
Sbjct: 260 NSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPEVTANMRSE 319
Query: 945 L 947
L
Sbjct: 320 L 320
>ref|XP_009757123.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
isoform X1 [Nicotiana sylvestris]
gi|698520618|ref|XP_009757124.1| PREDICTED: probable
(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2 isoform
X2 [Nicotiana sylvestris]
Length = 489
Score = 237 bits (605), Expect = 9e-60
Identities = 125/322 (38%), Positives = 206/322 (63%), Gaps = 7/322 (2%)
Frame = +3
Query: 3 IIFAILVLLTLASKTKHKNLPPGPRAWPLIGSLPMLFTNTHVPLHVTLANLAAAHGPIMT 182
I F +L+L + K+ NLPPGPR WP++G++ L N H+ ++ A HGP+++
Sbjct: 13 IPFFVLILKQIIPKSG--NLPPGPRPWPILGNILQLGKNPHI----SMTQFAKIHGPLIS 66
Query: 183 LWLGTQPTVIVSAPESAAEVLKTHDRACSGRYIPM--SFRLKHHMKYSLVWA-DCTDYWK 353
L LGTQ VI S+P+SAAE+LKT DR S R +P ++L ++SLVW+ D +++WK
Sbjct: 67 LRLGTQLVVIASSPDSAAEILKTQDRLLSARSVPRVAPYKLSVVDQHSLVWSSDLSNHWK 126
Query: 354 LLRKIARTEIFSPRMLQTQSRVREEKVGELMDYLRSRQGQAVKLSTFVFGTLLNILGNAV 533
LR RT +FS + +++Q+ +RE+KV E++++LRS++G+ VK+S +F T+LN LGN
Sbjct: 127 SLRAFCRTHLFSAKAVESQAALREKKVFEMIEFLRSKKGKTVKISEIMFATILNTLGNLF 186
Query: 534 CSKDVFVYSDDDHEGGKQGLQSMLQEMLMIGAELNIAEFYPILEGLDLQGLKRKSDDRFG 713
+KD+ D D+EG G++ ++++ + +GA NI+EFYP L+ LDLQGL++++ G
Sbjct: 187 FTKDL---CDLDYEGNTSGIKHVIRKFVELGAMPNISEFYPFLDALDLQGLRKQTKIYQG 243
Query: 714 RVMKLWEGTVXXXXXXXXXXS----KDMLDVLLANNFNDAQISALFWETFGPGSETSSTT 881
++ +W V S +D LD+++ + F D QI+ + E G++T+++T
Sbjct: 244 QLFNIWAEIVKEKRQAISRGSSNKIQDFLDIMIYSGFTDLQINIILMELIAAGTDTTTST 303
Query: 882 IEWVMAELIKNPDKTAKVRKEL 947
IEW MAEL++N + K++ EL
Sbjct: 304 IEWAMAELLRNKEVMHKLQAEL 325
>sp|Q9SP06.1|C80B3_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
AltName: Full=Cytochrome P450 80B1; AltName:
Full=Cytochrome P450 80B3
gi|6176562|gb|AAF05621.1|AF191772_1
(S)-N-methylcoclaurine 3'-hydroxylase [Papaver
somniferum]
Length = 481
Score = 236 bits (603), Expect = 1e-59
Identities = 119/301 (39%), Positives = 183/301 (60%), Gaps = 3/301 (0%)
Frame = +3
Query: 54 KNLPPGPRAWPLIGSLPMLFTNTHVPLHVTLANLAAAHGPIMTLWLGTQPTVIVSAPESA 233
K LPPGP+ WP++G+L L H A LA +G + +L LG++ V+ S P +A
Sbjct: 21 KGLPPGPKPWPIVGNLLQLGEKPHSQF----AQLAETYGDLFSLKLGSETVVVASTPLAA 76
Query: 234 AEVLKTHDRACSGRYIPMSFRLKHHMKYSLVWADCTDYWKLLRKIARTEIFSPRMLQTQS 413
+E+LKTHDR SGRY+ SFR+K H++ S+VW++C + WK LRK+ RTE+F+ +M+++Q+
Sbjct: 77 SEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTELFTQKMIESQA 136
Query: 414 RVREEKVGELMDYLRSRQGQAVKLSTFVFGTLLNILGNAVCSKDVFVYSDDDHEGGKQGL 593
VRE K E+++YL+ G VK++ VFGTL+NI GN + S+++F D+ G +
Sbjct: 137 EVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDE--SSGSVEM 194
Query: 594 QSMLQEMLMIGAELNIAEFYPILEGLDLQGLKRKSDDRFGRVMKLWEGTVXXXXXXXXXX 773
+ L ML +G N A+++P L DL G + D + +W +
Sbjct: 195 KEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLKERKIAKQHN 254
Query: 774 S---KDMLDVLLANNFNDAQISALFWETFGPGSETSSTTIEWVMAELIKNPDKTAKVRKE 944
+ D +++LL + +D QI+AL E FG G+ETS++TIEW ++EL KNP TA +R E
Sbjct: 255 NSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPQVTANMRLE 314
Query: 945 L 947
L
Sbjct: 315 L 315
>emb|CDP15158.1| unnamed protein product [Coffea canephora]
Length = 496
Score = 236 bits (602), Expect = 2e-59
Identities = 122/309 (39%), Positives = 195/309 (63%), Gaps = 6/309 (1%)
Frame = +3
Query: 42 KTKHKNLPPGPRAWPLIGSLPMLFTNTHVPLHVTLANLAAAHGPIMTLWLGTQPTVIVSA 221
K+++++LPPGP+ WP++G+LP + + HV LA LA +GP+++L LG+Q V+ S
Sbjct: 28 KSRNQSLPPGPKPWPIVGNLPQIGSKPHV----ALAQLAQDYGPLISLRLGSQLVVVGST 83
Query: 222 PESAAEVLKTHDRACSGRYIP-MSFRLKHHMKY-SLVWA-DCTDYWKLLRKIARTEIFSP 392
P +A E+LKTHDR SGR++P +S+ M Y S+ W +CTD WK LR + ++EI
Sbjct: 84 PAAATEILKTHDRILSGRHVPHVSYAKSPLMNYVSVGWTYECTDQWKFLRTLCKSEILGA 143
Query: 393 RMLQTQSRVREEKVGELMDYLRSRQGQAVKLSTFVFGTLLNILGNAVCSKDVFVYSDDDH 572
++++ QS +RE+K EL+ +L S++GQ +K++ VF ++ N LG SKD Y + ++
Sbjct: 144 KVIENQSHLREQKANELVQFLVSKEGQRIKIAEVVFVSVFNFLGQIFFSKDFLSYDEVEN 203
Query: 573 EGGKQGLQSMLQEMLMIGAELNIAEFYPILEGLDLQGLKRKSDDRFGRVMKLWEGTVXXX 752
G G+ +++E++ + NI++ YP+L GLDLQ L +K+ ++ W+ +
Sbjct: 204 GG---GMSELIREVMELWTAPNISDLYPVLGGLDLQRLSKKASVCHNKICSAWQEIIRER 260
Query: 753 XXXXXXXS---KDMLDVLLANNFNDAQISALFWETFGPGSETSSTTIEWVMAELIKNPDK 923
S KD LDVLL N+F+D QI+ L E F GS+TS++T+EW MAEL++NP
Sbjct: 261 RGKKYQDSTRQKDFLDVLLQNDFSDDQINYLHLELFAAGSDTSTSTVEWAMAELLRNPKF 320
Query: 924 TAKVRKELD 950
+R+ELD
Sbjct: 321 LDMIRQELD 329
>emb|CBI31830.3| unnamed protein product [Vitis vinifera]
Length = 1538
Score = 235 bits (600), Expect = 3e-59
Identities = 131/301 (43%), Positives = 184/301 (61%), Gaps = 5/301 (1%)
Frame = +3
Query: 60 LPPGPRAWPLIGSLPMLFTNTHVPLHVTLANLAAAHGPIMTLWLGTQPTVIVSAPESAAE 239
LPPGP WP+IG+L + N H L ANLA HGP+M+L LGTQ V+ S+P +A E
Sbjct: 578 LPPGPYPWPIIGNLLQMGKNPHAKL----ANLAKLHGPLMSLRLGTQLMVVASSPAAAME 633
Query: 240 VLKTHDRACSGRYIPMSFRLKHHM--KYSLVWA-DCTDYWKLLRKIARTEIFSPRMLQTQ 410
VLKTHDRA SGRY+ S +K+ S+V+A DC WK LR I R E+FS + +++Q
Sbjct: 634 VLKTHDRALSGRYLSSSVPVKNPKLNHLSIVFAKDCNTNWKNLRAICRMELFSGKAMESQ 693
Query: 411 SRVREEKVGELMDYLRSRQGQAVKLSTFVFGTLLNILGNAVCSKDVFVYSDDDHEGGKQG 590
+RE KV EL+++L +++G+ VK+ VF T+ NIL N S D+ + D+ GG
Sbjct: 694 VELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDEGRVGG--A 751
Query: 591 LQSMLQEMLMIGAELNIAEFYPILEGLDLQGLKRKSDDRFGRVMKLWEGTVXXXXXXXXX 770
L+ ++ + GA N++++YPIL GLD+QG+ RK+ + F R+ WE +
Sbjct: 752 LKDLIHKNAEFGATPNLSDYYPILGGLDIQGINRKAKEMFERIPTTWEDILKERRTQRSN 811
Query: 771 XS--KDMLDVLLANNFNDAQISALFWETFGPGSETSSTTIEWVMAELIKNPDKTAKVRKE 944
S +D L+ LL F D QI+ + E F G+ETSS T+EW MAELI+N D K+R E
Sbjct: 812 RSSHRDFLEALLEIGFEDDQINQVILELFSAGAETSSLTVEWAMAELIRNQDAMDKLRGE 871
Query: 945 L 947
L
Sbjct: 872 L 872
Score = 234 bits (597), Expect = 7e-59
Identities = 129/303 (42%), Positives = 188/303 (62%), Gaps = 7/303 (2%)
Frame = +3
Query: 60 LPPGPRAWPLIGSLPMLFTNTHVPLHVTLANLAAAHGPIMTLWLGTQPTVIVSAPESAAE 239
LPPGP WP+IG+L L N HV L A+LA HGP+M+L LGTQ V+ S+P +A E
Sbjct: 1083 LPPGPYPWPIIGNLLQLGKNPHVKL----ASLAKLHGPLMSLRLGTQLMVVASSPAAALE 1138
Query: 240 VLKTHDRACSGRYIPMSFRLK----HHMKYSLVWA-DCTDYWKLLRKIARTEIFSPRMLQ 404
VLKTHDR SGRY+ S +K +H+ SL +A +CT+ WK LR I RTE+FS + ++
Sbjct: 1139 VLKTHDRTLSGRYVSSSLSVKDPKLNHL--SLAFAKECTNNWKNLRTICRTEMFSGKAME 1196
Query: 405 TQSRVREEKVGELMDYLRSRQGQAVKLSTFVFGTLLNILGNAVCSKDVFVYSDDDHEGGK 584
+ +RE KV EL+++L +++G+ VK+ VF T+ NIL N S D+ + +
Sbjct: 1197 SHVELRERKVMELVEFLATKEGEVVKVMDLVFTTICNILSNTFFSMDLCDFE-------R 1249
Query: 585 QGLQSMLQEMLMIGAELNIAEFYPILEGLDLQGLKRKSDDRFGRVMKLWEGTVXXXXXXX 764
+GL+ + +GA N+++FYPIL+GL+L G K+KS + GR++ WEGT+
Sbjct: 1250 EGLKDFIYRAAELGATPNLSDFYPILDGLNLHGSKKKSKEALGRILATWEGTLKERRKQK 1309
Query: 765 XXXS--KDMLDVLLANNFNDAQISALFWETFGPGSETSSTTIEWVMAELIKNPDKTAKVR 938
S +D+L+ L F D QI+ + E F G++TS+ TIEW + +LI+NPD K+R
Sbjct: 1310 NPGSSHRDLLEAFLEIRFEDDQINQVILELFSAGADTSTLTIEWAITQLIRNPDVMYKLR 1369
Query: 939 KEL 947
EL
Sbjct: 1370 DEL 1372
Score = 213 bits (541), Expect = 2e-52
Identities = 128/306 (41%), Positives = 175/306 (57%), Gaps = 6/306 (1%)
Frame = +3
Query: 48 KHKNLPPGPRAWPLIGSLPMLFTNTHVPLHVTLANLAAAHGPIMTLWLGTQPTVIVSAPE 227
K LPPGP WP+IG+L + N LHV LANLA HGP+M+L LGTQ V+ S+
Sbjct: 34 KSPPLPPGPYPWPIIGNLLQMGGN----LHVKLANLAKRHGPLMSLRLGTQIMVVASSSA 89
Query: 228 SAAEVLKTHDRACSGRY----IPMSFRLKHHMKYSLVWADCTDYWKLLRKIARTEIFSPR 395
+A EVLKTHDR SGRY IP++ +H+ + C W+ L+ I R E+FS +
Sbjct: 90 AAMEVLKTHDRTLSGRYVSTTIPVNSPKLNHLAMAFAKV-CNSDWRNLKAICRMELFSGK 148
Query: 396 MLQTQSRVREEKVGELMDYLRSRQGQAVKLSTFVFGTLLNILGNAVCSKDVFVYSDDDHE 575
++++ +RE KV EL+++L ++G+ VK+ V+ T+ NIL N S D + D
Sbjct: 149 AMESRVELRERKVMELVEFLEKKEGEVVKVMDLVYTTVCNILSNKFFSIDFSDFEGRDVR 208
Query: 576 GGKQGLQSMLQEMLMIGAELNIAEFYPILEGLDLQGLKRKSDDRFGRVMKLWEGTVXXXX 755
G L+ +L E +GA NI +FYPIL GLD+QG+++K + F R+ WE +
Sbjct: 209 GVL--LKDLLNENAELGA-TNILDFYPILGGLDIQGIRKKLKEIFRRIPTTWEDILKERR 265
Query: 756 XXXXXXSK--DMLDVLLANNFNDAQISALFWETFGPGSETSSTTIEWVMAELIKNPDKTA 929
S D LD LL F D QI+ + E F G ETSS T+EW MAELIKN D
Sbjct: 266 KQRIHGSSHGDFLDALLETGFEDDQINHVIMELFFAGPETSSLTVEWAMAELIKNQDAMH 325
Query: 930 KVRKEL 947
K+ EL
Sbjct: 326 KLCNEL 331
>gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
Length = 481
Score = 235 bits (600), Expect = 3e-59
Identities = 118/301 (39%), Positives = 183/301 (60%), Gaps = 3/301 (0%)
Frame = +3
Query: 54 KNLPPGPRAWPLIGSLPMLFTNTHVPLHVTLANLAAAHGPIMTLWLGTQPTVIVSAPESA 233
K LPPGP+ WP++G+L L H A LA +G + +L LG++ V+ S P +A
Sbjct: 21 KGLPPGPKPWPIVGNLLQLGEKPHSQF----AQLAETYGDLFSLKLGSETVVVASTPLAA 76
Query: 234 AEVLKTHDRACSGRYIPMSFRLKHHMKYSLVWADCTDYWKLLRKIARTEIFSPRMLQTQS 413
+E+LKTHDR SGRY+ SFR+K H++ S+VW++C + WK LRK+ RT++F+ +M+++Q+
Sbjct: 77 SEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTDLFTQKMIESQA 136
Query: 414 RVREEKVGELMDYLRSRQGQAVKLSTFVFGTLLNILGNAVCSKDVFVYSDDDHEGGKQGL 593
VRE K E+++YL+ G VK++ VFGTL+NI GN + S+++F D+ G +
Sbjct: 137 EVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDE--SSGSVEM 194
Query: 594 QSMLQEMLMIGAELNIAEFYPILEGLDLQGLKRKSDDRFGRVMKLWEGTVXXXXXXXXXX 773
+ L ML +G N A+++P L DL G + D + +W +
Sbjct: 195 KEHLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLKESKIAKQHN 254
Query: 774 S---KDMLDVLLANNFNDAQISALFWETFGPGSETSSTTIEWVMAELIKNPDKTAKVRKE 944
+ D +++LL + +D QI+AL E FG G+ETS++TIEW ++EL KNP TA +R E
Sbjct: 255 NSKKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPQVTANMRLE 314
Query: 945 L 947
L
Sbjct: 315 L 315