BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Aconitum23_contig00036049
         (397 letters)

Database: ./nr 
           77,306,371 sequences; 28,104,191,420 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004147743.1| PREDICTED: DNA topoisomerase 3-alpha-like [C...    68   2e-09
ref|XP_006292441.1| hypothetical protein CARUB_v10018659mg [Caps...    68   3e-09
ref|XP_011623466.1| PREDICTED: uncharacterized protein LOC184345...    67   4e-09
ref|XP_012067571.1| PREDICTED: uncharacterized protein LOC105630...    67   5e-09
gb|ABA18102.1| zinc knuckle family protein [Olimarabidopsis pumila]    67   7e-09
ref|XP_011004266.1| PREDICTED: uncharacterized protein LOC105110...    66   9e-09
ref|XP_011004265.1| PREDICTED: uncharacterized protein LOC105110...    66   9e-09
ref|XP_002875595.1| zinc knuckle family protein [Arabidopsis lyr...    66   9e-09
ref|XP_012067382.1| PREDICTED: uncharacterized protein LOC105630...    65   3e-08
ref|XP_012067381.1| PREDICTED: uncharacterized protein LOC105630...    65   3e-08
ref|XP_011048911.1| PREDICTED: uncharacterized protein LOC105142...    65   3e-08
gb|KFK40133.1| hypothetical protein AALP_AA3G335000 [Arabis alpina]    65   3e-08
gb|KDP41875.1| hypothetical protein JCGZ_26893 [Jatropha curcas]       65   3e-08
ref|XP_006401381.1| hypothetical protein EUTSA_v10013799mg [Eutr...    65   3e-08
ref|NP_189872.1| CCHC-type zinc knuckle protein [Arabidopsis tha...    65   3e-08
ref|XP_010535814.1| PREDICTED: DNA topoisomerase 3-alpha [Tarena...    64   6e-08
ref|XP_013666882.1| PREDICTED: cold shock domain-containing prot...    63   8e-08
ref|XP_013666881.1| PREDICTED: DNA-binding protein HEXBP-like is...    63   8e-08
ref|XP_009120119.1| PREDICTED: DNA-binding protein HEXBP [Brassi...    63   8e-08
ref|XP_013675650.1| PREDICTED: cold shock protein 1-like [Brassi...    63   8e-08

>ref|XP_004147743.1| PREDICTED: DNA topoisomerase 3-alpha-like [Cucumis sativus]
           gi|700198347|gb|KGN53505.1| hypothetical protein
           Csa_4G062410 [Cucumis sativus]
          Length = 327

 Score = 68.2 bits (165), Expect = 2e-09
 Identities = 37/122 (30%), Positives = 49/122 (40%), Gaps = 20/122 (16%)
 Frame = -3

Query: 338 CVCGAGYCRVLVSSTSRTSDRRYITCPNMRGNIKAYGLWQWLDEVRM------------- 198
           C CG+G C VL ++T R   R++  CP  + N    G ++W D   +             
Sbjct: 129 CPCGSGICSVLTANTERNRGRKFYKCPVRQEN-GGCGFFEWCDSASVANVVSYGSQNPLS 187

Query: 197 -------CPCNAGICKYFLSHLKANPDRWFYCCPKSSVGVRECGYFAWKTSKSKEAKKDN 39
                  CPC AG CK   +    N  + FYCCP S      C +F W    S E K   
Sbjct: 188 SSFSDLQCPCGAGSCKILTAKTGNNVGKQFYCCPSSQA---SCRFFRWCEEPSMETKNQE 244

Query: 38  VT 33
            T
Sbjct: 245 ST 246


>ref|XP_006292441.1| hypothetical protein CARUB_v10018659mg [Capsella rubella]
           gi|482561148|gb|EOA25339.1| hypothetical protein
           CARUB_v10018659mg [Capsella rubella]
          Length = 433

 Score = 67.8 bits (164), Expect = 3e-09
 Identities = 38/115 (33%), Positives = 48/115 (41%), Gaps = 19/115 (16%)
 Frame = -3

Query: 338 CVCGAGYCRVLVSSTSRTSDRRYITCPNMRGNIKAYGLWQWLDEVR-------------- 201
           C CG G C VL S+T +   R++  CPN   N    G +QW D V+              
Sbjct: 95  CPCGVGLCLVLTSNTQKNPGRKFYKCPNREEN-GGCGFFQWCDAVQSSGTSHSYGNNNDS 153

Query: 200 -----MCPCNAGICKYFLSHLKANPDRWFYCCPKSSVGVRECGYFAWKTSKSKEA 51
                 CPC AG+C+   +    N  R FY CP   V    CG+F W    S  A
Sbjct: 154 KFPDHQCPCGAGLCRVLTAKTGENVGRQFYRCP---VFEGSCGFFKWCNDDSVSA 205


>ref|XP_011623466.1| PREDICTED: uncharacterized protein LOC18434520 [Amborella
           trichopoda]
          Length = 424

 Score = 67.4 bits (163), Expect = 4e-09
 Identities = 37/118 (31%), Positives = 52/118 (44%), Gaps = 11/118 (9%)
 Frame = -3

Query: 353 TVRLQCVCGAGYCRVLVSSTSRTSDRRYITCPNMRGNIKAYGLWQWLD-----------E 207
           ++ +QC CG G C V +S + +   R+Y TCP      +  G +QW D           +
Sbjct: 88  SLEVQCPCGGGPCPVSISKSRQNPGRKYYTCP----MYQRCGFFQWCDVPSLPQSGSMLK 143

Query: 206 VRMCPCNAGICKYFLSHLKANPDRWFYCCPKSSVGVRECGYFAWKTSKSKEAKKDNVT 33
             +C C AG CK F S    NP R F+ CP    G   C +F W     K     N++
Sbjct: 144 FPICGCRAGECKLFTSKTDKNPGRQFFVCPIKK-GQGACNFFLWLEDYEKGLPGSNMS 200


>ref|XP_012067571.1| PREDICTED: uncharacterized protein LOC105630379 [Jatropha curcas]
          Length = 287

 Score = 67.0 bits (162), Expect = 5e-09
 Identities = 37/101 (36%), Positives = 54/101 (53%), Gaps = 7/101 (6%)
 Frame = -3

Query: 344 LQCVCGAGYCRVLVSSTSRTSDRRYITCPNMRGNIKAYGLWQWLDEVR----MCPCNAGI 177
           ++C CG G CR+L S T +   RR+ TCP  + +    G ++W DE+     +CPC AG 
Sbjct: 41  VRCPCGRGKCRILTSYTPKNPKRRFYTCPVDKSS-GPCGFFKWCDELSNKAPLCPCGAGT 99

Query: 176 CKYFLSHLKANPD---RWFYCCPKSSVGVRECGYFAWKTSK 63
           C+  L+   + PD   R+F C  K S G   C +F W  S+
Sbjct: 100 CR--LTRCFSGPDKGRRYFACRVKKSQGA--CSFFQWADSE 136


>gb|ABA18102.1| zinc knuckle family protein [Olimarabidopsis pumila]
          Length = 369

 Score = 66.6 bits (161), Expect = 7e-09
 Identities = 35/107 (32%), Positives = 46/107 (42%), Gaps = 19/107 (17%)
 Frame = -3

Query: 338 CVCGAGYCRVLVSSTSRTSDRRYITCPNMRGNIKAYGLWQWLDEVR-------------- 201
           C CG G C +L S+T +   R++  CPN   N    G +QW D V+              
Sbjct: 92  CPCGVGLCLILTSNTQKNPGRKFYKCPNREEN-GGCGFFQWCDAVQSSGTTHSYGNTNDS 150

Query: 200 -----MCPCNAGICKYFLSHLKANPDRWFYCCPKSSVGVRECGYFAW 75
                 CPC AG+C+   +    N  R FY CP   V    CG+F W
Sbjct: 151 KFPDHQCPCGAGLCRVLTAKTGENVGRQFYRCP---VFEGSCGFFKW 194


>ref|XP_011004266.1| PREDICTED: uncharacterized protein LOC105110803 isoform X2 [Populus
           euphratica]
          Length = 607

 Score = 66.2 bits (160), Expect = 9e-09
 Identities = 33/101 (32%), Positives = 50/101 (49%), Gaps = 7/101 (6%)
 Frame = -3

Query: 344 LQCVCGAGYCRVLVSSTSRTSDRRYITCPNMRGNIKAYGLWQWLDEVR------MCPCNA 183
           ++C CG G C+V  S+T +   R++  CP  R    + G ++W D++       MCPC A
Sbjct: 123 VRCPCGRGTCKVSTSNTVKNPGRKFYACPVDRRTSGSCGFFKWSDDIAARFKPPMCPCGA 182

Query: 182 GICKYFLSHLKANPDRWFYCCP-KSSVGVRECGYFAWKTSK 63
           G C   L     + DRW++ C  K   G   C +F W  S+
Sbjct: 183 GSCSLNLVSSGPDRDRWYFACRIKKKHGA--CKFFQWADSE 221


>ref|XP_011004265.1| PREDICTED: uncharacterized protein LOC105110803 isoform X1 [Populus
           euphratica]
          Length = 602

 Score = 66.2 bits (160), Expect = 9e-09
 Identities = 33/101 (32%), Positives = 50/101 (49%), Gaps = 7/101 (6%)
 Frame = -3

Query: 344 LQCVCGAGYCRVLVSSTSRTSDRRYITCPNMRGNIKAYGLWQWLDEVR------MCPCNA 183
           ++C CG G C+V  S+T +   R++  CP  R    + G ++W D++       MCPC A
Sbjct: 123 VRCPCGRGTCKVSTSNTVKNPGRKFYACPVDRRTSGSCGFFKWSDDIAARFKPPMCPCGA 182

Query: 182 GICKYFLSHLKANPDRWFYCCP-KSSVGVRECGYFAWKTSK 63
           G C   L     + DRW++ C  K   G   C +F W  S+
Sbjct: 183 GSCSLNLVSSGPDRDRWYFACRIKKKHGA--CKFFQWADSE 221


>ref|XP_002875595.1| zinc knuckle family protein [Arabidopsis lyrata subsp. lyrata]
           gi|297321433|gb|EFH51854.1| zinc knuckle family protein
           [Arabidopsis lyrata subsp. lyrata]
          Length = 337

 Score = 66.2 bits (160), Expect = 9e-09
 Identities = 36/108 (33%), Positives = 46/108 (42%), Gaps = 20/108 (18%)
 Frame = -3

Query: 338 CVCGAGYCRVLVSSTSRTSDRRYITCPNMRGNIKAYGLWQWLDEVR-------------- 201
           C CG G C VL S+T +   R++  CPN   N    G +QW D V+              
Sbjct: 95  CPCGVGLCLVLTSTTPKNPGRKFYKCPNREEN-GGCGFFQWCDAVQSSGTTTHSYGNTND 153

Query: 200 ------MCPCNAGICKYFLSHLKANPDRWFYCCPKSSVGVRECGYFAW 75
                  CPC AG+C+   +    N  R FY CP   V    CG+F W
Sbjct: 154 SKFPDHQCPCGAGLCRVLTAKTGENVGRQFYRCP---VFEGSCGFFKW 198


>ref|XP_012067382.1| PREDICTED: uncharacterized protein LOC105630230 isoform X2
           [Jatropha curcas]
          Length = 603

 Score = 64.7 bits (156), Expect = 3e-08
 Identities = 27/94 (28%), Positives = 48/94 (51%), Gaps = 4/94 (4%)
 Frame = -3

Query: 389 LRESRKKMSTSETVRLQCVCGAGYCRVLVSSTSRTSDRRYITCPNMRGNIKAYGLWQWLD 210
           L  S   ++T + + ++C CG G CR+  S T    +R++ TCP  + +  + G ++W D
Sbjct: 69  LTSSSSSLNTCDVLLIRCPCGRGKCRIFTSHTQANPERKFYTCPVDKSS-GSCGFFKWCD 127

Query: 209 EVR----MCPCNAGICKYFLSHLKANPDRWFYCC 120
           E+     +CPC AG C           ++W++ C
Sbjct: 128 ELSITPPICPCGAGFCSLNRDSTSPYEEKWYFAC 161


>ref|XP_012067381.1| PREDICTED: uncharacterized protein LOC105630230 isoform X1
           [Jatropha curcas]
          Length = 610

 Score = 64.7 bits (156), Expect = 3e-08
 Identities = 27/94 (28%), Positives = 48/94 (51%), Gaps = 4/94 (4%)
 Frame = -3

Query: 389 LRESRKKMSTSETVRLQCVCGAGYCRVLVSSTSRTSDRRYITCPNMRGNIKAYGLWQWLD 210
           L  S   ++T + + ++C CG G CR+  S T    +R++ TCP  + +  + G ++W D
Sbjct: 69  LTSSSSSLNTCDVLLIRCPCGRGKCRIFTSHTQANPERKFYTCPVDKSS-GSCGFFKWCD 127

Query: 209 EVR----MCPCNAGICKYFLSHLKANPDRWFYCC 120
           E+     +CPC AG C           ++W++ C
Sbjct: 128 ELSITPPICPCGAGFCSLNRDSTSPYEEKWYFAC 161


>ref|XP_011048911.1| PREDICTED: uncharacterized protein LOC105142804 [Populus
           euphratica]
          Length = 316

 Score = 64.7 bits (156), Expect = 3e-08
 Identities = 32/101 (31%), Positives = 51/101 (50%), Gaps = 7/101 (6%)
 Frame = -3

Query: 344 LQCVCGAGYCRVLVSSTSRTSDRRYITCPNMRGNIKAYGLWQWLDEV------RMCPCNA 183
           ++C CG G C+V  S+T +   R++  CP  R    + G ++W D++       MCPC A
Sbjct: 123 VRCPCGRGTCKVSTSNTVKNPGRKFYACPVDRRTSGSCGFFRWSDDIAARFKPAMCPCGA 182

Query: 182 GICKYFLSHLKANPDRWFYCCP-KSSVGVRECGYFAWKTSK 63
           G C   +     + DRW++ C  K + G   C +F W  S+
Sbjct: 183 GSCSLNIVSSCPDRDRWYFTCRIKKNHGA--CKFFQWAGSE 221


>gb|KFK40133.1| hypothetical protein AALP_AA3G335000 [Arabis alpina]
          Length = 414

 Score = 64.7 bits (156), Expect = 3e-08
 Identities = 36/115 (31%), Positives = 48/115 (41%), Gaps = 18/115 (15%)
 Frame = -3

Query: 365 STSETVRLQCVCGAGYCRVLVSSTSRTSDRRYITCPNMRGNIKAYGLWQWLDEVR----- 201
           S  E     C CG+G C +L S+T +   R++  CPN   N    G +QW D V+     
Sbjct: 90  SDQEFPEKSCSCGSGVCLILTSNTQKNPGRKFYKCPNKEDN-GGCGFFQWCDAVQSSGTQ 148

Query: 200 -------------MCPCNAGICKYFLSHLKANPDRWFYCCPKSSVGVRECGYFAW 75
                         CPC AG+ +   +    N  R FY CP   V    CG+F W
Sbjct: 149 SYGNTNDSKFPELQCPCGAGLSRILTAKTGENVGRQFYRCP---VFEGSCGFFKW 200


>gb|KDP41875.1| hypothetical protein JCGZ_26893 [Jatropha curcas]
          Length = 609

 Score = 64.7 bits (156), Expect = 3e-08
 Identities = 27/94 (28%), Positives = 48/94 (51%), Gaps = 4/94 (4%)
 Frame = -3

Query: 389 LRESRKKMSTSETVRLQCVCGAGYCRVLVSSTSRTSDRRYITCPNMRGNIKAYGLWQWLD 210
           L  S   ++T + + ++C CG G CR+  S T    +R++ TCP  + +  + G ++W D
Sbjct: 68  LTSSSSSLNTCDVLLIRCPCGRGKCRIFTSHTQANPERKFYTCPVDKSS-GSCGFFKWCD 126

Query: 209 EVR----MCPCNAGICKYFLSHLKANPDRWFYCC 120
           E+     +CPC AG C           ++W++ C
Sbjct: 127 ELSITPPICPCGAGFCSLNRDSTSPYEEKWYFAC 160


>ref|XP_006401381.1| hypothetical protein EUTSA_v10013799mg [Eutrema salsugineum]
           gi|557102471|gb|ESQ42834.1| hypothetical protein
           EUTSA_v10013799mg [Eutrema salsugineum]
          Length = 384

 Score = 64.7 bits (156), Expect = 3e-08
 Identities = 35/111 (31%), Positives = 46/111 (41%), Gaps = 23/111 (20%)
 Frame = -3

Query: 338 CVCGAGYCRVLVSSTSRTSDRRYITCPNMRGNIKAYGLWQWLDEVR-------------- 201
           C CG G C +L S+T +   R++  CPN   N    G +QW D V+              
Sbjct: 103 CPCGVGVCLILTSNTQKNPGRKFYKCPNREEN-GGCGFFQWCDAVQSSGTQPGTHHSYGN 161

Query: 200 ---------MCPCNAGICKYFLSHLKANPDRWFYCCPKSSVGVRECGYFAW 75
                     CPC AG+C+   +    N  R FY CP   V    CG+F W
Sbjct: 162 TNESKFPDHQCPCGAGLCRVLTAKTGENIGRQFYRCP---VFEGSCGFFKW 209


>ref|NP_189872.1| CCHC-type zinc knuckle protein [Arabidopsis thaliana]
           gi|7529257|emb|CAB86673.1| putative protein [Arabidopsis
           thaliana] gi|17529084|gb|AAL38752.1| unknown protein
           [Arabidopsis thaliana] gi|22136980|gb|AAM91719.1|
           unknown protein [Arabidopsis thaliana]
           gi|332644235|gb|AEE77756.1| CCHC-type zinc knuckle
           protein [Arabidopsis thaliana]
          Length = 372

 Score = 64.7 bits (156), Expect = 3e-08
 Identities = 35/111 (31%), Positives = 46/111 (41%), Gaps = 23/111 (20%)
 Frame = -3

Query: 338 CVCGAGYCRVLVSSTSRTSDRRYITCPNMRGNIKAYGLWQWLDEVR-------------- 201
           C CG G C +L S+T +   R++  CPN   N    G +QW D V+              
Sbjct: 96  CPCGVGICLILTSNTPKNPGRKFYKCPNREEN-GGCGFFQWCDAVQSSGTSTTTSNSYGN 154

Query: 200 ---------MCPCNAGICKYFLSHLKANPDRWFYCCPKSSVGVRECGYFAW 75
                     CPC AG+C+   +    N  R FY CP   V    CG+F W
Sbjct: 155 GNDTKFPDHQCPCGAGLCRVLTAKTGENVGRQFYRCP---VFEGSCGFFKW 202


>ref|XP_010535814.1| PREDICTED: DNA topoisomerase 3-alpha [Tarenaya hassleriana]
           gi|729328398|ref|XP_010535815.1| PREDICTED: DNA
           topoisomerase 3-alpha [Tarenaya hassleriana]
          Length = 329

 Score = 63.5 bits (153), Expect = 6e-08
 Identities = 36/132 (27%), Positives = 51/132 (38%), Gaps = 22/132 (16%)
 Frame = -3

Query: 338 CVCGAGYCRVLVSSTSRTSDRRYITCPNMRGNIKAYGLWQWLDEVR-------------- 201
           C CG G C +L S+T +   R++  CP +R      G +QW DE R              
Sbjct: 120 CTCGVGICLILTSNTQKNPGRKFYKCP-VREENGGCGFFQWCDEDRSSGTHAGTHSYANS 178

Query: 200 --------MCPCNAGICKYFLSHLKANPDRWFYCCPKSSVGVRECGYFAWKTSKSKEAKK 45
                    CPC +G C+   +    N  R FY CP        CG+F W    S  +  
Sbjct: 179 NDSKFPDHQCPCGSGSCRILTAKTGQNIGRQFYRCPAFEA---SCGFFKWCDDTSVASSM 235

Query: 44  DNVTKEDKEFEE 9
           +N   + +   E
Sbjct: 236 ENSFAKTRNLSE 247


>ref|XP_013666882.1| PREDICTED: cold shock domain-containing protein 3-like isoform X2
           [Brassica napus]
          Length = 379

 Score = 63.2 bits (152), Expect = 8e-08
 Identities = 35/108 (32%), Positives = 45/108 (41%), Gaps = 20/108 (18%)
 Frame = -3

Query: 338 CVCGAGYCRVLVSSTSRTSDRRYITCPNMRGNIKAYGLWQWLDEVR-------------- 201
           C CGAG C +L S+T +   R++  CPN   N    G +QW D V+              
Sbjct: 98  CPCGAGVCSILTSNTQKNPGRKFYKCPNREEN-GGCGFFQWCDAVQSSGTQPGSYGNTNE 156

Query: 200 ------MCPCNAGICKYFLSHLKANPDRWFYCCPKSSVGVRECGYFAW 75
                  CPC AG  +   +    N  R FY CP   V    CG+F W
Sbjct: 157 SKFPDLQCPCGAGFSRVLTAKTGENIGRQFYRCP---VFEGSCGFFKW 201


>ref|XP_013666881.1| PREDICTED: DNA-binding protein HEXBP-like isoform X1 [Brassica
           napus]
          Length = 415

 Score = 63.2 bits (152), Expect = 8e-08
 Identities = 35/108 (32%), Positives = 45/108 (41%), Gaps = 20/108 (18%)
 Frame = -3

Query: 338 CVCGAGYCRVLVSSTSRTSDRRYITCPNMRGNIKAYGLWQWLDEVR-------------- 201
           C CGAG C +L S+T +   R++  CPN   N    G +QW D V+              
Sbjct: 98  CPCGAGVCSILTSNTQKNPGRKFYKCPNREEN-GGCGFFQWCDAVQSSGTQPGSYGNTNE 156

Query: 200 ------MCPCNAGICKYFLSHLKANPDRWFYCCPKSSVGVRECGYFAW 75
                  CPC AG  +   +    N  R FY CP   V    CG+F W
Sbjct: 157 SKFPDLQCPCGAGFSRVLTAKTGENIGRQFYRCP---VFEGSCGFFKW 201


>ref|XP_009120119.1| PREDICTED: DNA-binding protein HEXBP [Brassica rapa]
          Length = 415

 Score = 63.2 bits (152), Expect = 8e-08
 Identities = 35/108 (32%), Positives = 45/108 (41%), Gaps = 20/108 (18%)
 Frame = -3

Query: 338 CVCGAGYCRVLVSSTSRTSDRRYITCPNMRGNIKAYGLWQWLDEVR-------------- 201
           C CGAG C +L S+T +   R++  CPN   N    G +QW D V+              
Sbjct: 98  CPCGAGVCSILTSNTQKNPGRKFYKCPNREEN-GGCGFFQWCDAVQSSGTQPGSYGNTNE 156

Query: 200 ------MCPCNAGICKYFLSHLKANPDRWFYCCPKSSVGVRECGYFAW 75
                  CPC AG  +   +    N  R FY CP   V    CG+F W
Sbjct: 157 SKFPDLQCPCGAGFSRVLTAKTGENIGRQFYRCP---VFEGSCGFFKW 201


>ref|XP_013675650.1| PREDICTED: cold shock protein 1-like [Brassica napus]
           gi|674875081|emb|CDY57965.1| BnaCnng32640D [Brassica
           napus]
          Length = 377

 Score = 63.2 bits (152), Expect = 8e-08
 Identities = 37/130 (28%), Positives = 55/130 (42%), Gaps = 24/130 (18%)
 Frame = -3

Query: 341 QCVCGAGYCRVLVSSTSRTSDRRYITCPNMRGNIKAYGLWQWLDEVR------------- 201
           +C CG G C +L S+T +   R++  CPN   N    G +QW D V+             
Sbjct: 98  KCPCGNGVCSILTSNTQKNPGRKFYKCPNREEN-GGCGFFQWCDAVQSSGTQPGSYGNTN 156

Query: 200 -------MCPCNAGICKYFLSHLKANPDRWFYCCPKSSVGVRECGYFAWKTSKSKEA--- 51
                   CPC AG+ +   +    N  R FY CP   V    CG+F W    + ++   
Sbjct: 157 ESKFPDLQCPCGAGLSRVLTAKTGENIGRQFYRCP---VFEGSCGFFKWCNDDAVQSPSF 213

Query: 50  -KKDNVTKED 24
            K  N+++ D
Sbjct: 214 TKSSNLSESD 223