BLASTX nr result
ID: Aconitum23_contig00033832
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Aconitum23_contig00033832
(1654 letters)
Database: ./nr
77,306,371 sequences; 28,104,191,420 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. diss... 469 0.0
gb|AJD20229.1| corytuberine synthase [Sinopodophyllum hexandrum] 402 e-176
sp|P47195.1|C80A1_BERST RecName: Full=Berbamunine synthase; AltN... 296 e-128
ref|XP_010253990.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 280 e-126
gb|AGC29949.1| CYP80G3 [Sinopodophyllum hexandrum] 245 e-123
ref|XP_010253989.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 264 e-120
gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase ... 260 e-114
sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-... 258 e-114
sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-... 258 e-113
gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum... 246 e-112
gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [P... 248 e-112
gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [... 244 e-111
emb|CBI34714.3| unnamed protein product [Vitis vinifera] 238 e-111
ref|XP_002283461.2| PREDICTED: probable (S)-N-methylcoclaurine 3... 238 e-111
sp|Q9SP06.1|C80B3_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-... 245 e-111
ref|XP_009609252.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 246 e-111
ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym... 239 e-111
emb|CBI31830.3| unnamed protein product [Vitis vinifera] 244 e-110
ref|XP_002283415.2| PREDICTED: probable (S)-N-methylcoclaurine 3... 234 e-110
ref|XP_010662515.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 241 e-110
>dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. dissecta]
Length = 486
Score = 469 bits (1206), Expect(2) = 0.0
Identities = 235/320 (73%), Positives = 270/320 (84%)
Frame = +2
Query: 86 MDIQIATYCIIFAILVMLALASKTKHKNLPPGPSAWPLIGSLPMLFTNTQVPLHVTLTNL 265
MD+QIA + +I ILV + L K K+KNLPPGP WPLIG+LP+LFTNT+VPLH+TL N+
Sbjct: 1 MDLQIALFSLIPVILVFILLL-KPKYKNLPPGPHPWPLIGNLPILFTNTEVPLHITLANM 59
Query: 266 AAAHGPIMTLWLGTQPTVIASTPESAAEVLKTNDRVCSGRYIPMSFRLKHHIKHSLVWAD 445
A HGPIM LWLGTQPTV+AST E+A E+LKT+DR+ S R+I MSFRLKHHIK+SLVW+D
Sbjct: 60 ARTHGPIMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHIRMSFRLKHHIKYSLVWSD 119
Query: 446 CTDNWKLLRKIARTEIFSPKMLQTQSRVREEKAGELVDFLRSKQGQAVKLSTFVFGTLLN 625
CTD WKLLRKI RTEIFSPKMLQ QS VRE+K EL+DFLRSK+GQ VK+S FVFGTLLN
Sbjct: 120 CTDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVKISQFVFGTLLN 179
Query: 626 ILGNAVYSEDVFVYSDDEGGKQGIQSMIKEMLMIGAEPNLAEFYPILEGLDLQGLKRKCD 805
ILGN V+S+DVFVY DE K GIQ++I+EMLMIGAEPN+AEFYP LE LDLQGLK+KCD
Sbjct: 180 ILGNVVFSKDVFVYG-DETDKGGIQNLIREMLMIGAEPNVAEFYPSLEELDLQGLKKKCD 238
Query: 806 DRFVRVMKLWEGTVXXXXXXXXXXXXXDMLDVLLANDFNDAQINALFLETFGPGSETSSA 985
+RF+RVMK+WEGTV DMLDVLLANDFNDAQINALFLETFGPGSETSSA
Sbjct: 239 ERFIRVMKMWEGTV-KERKANRNEESKDMLDVLLANDFNDAQINALFLETFGPGSETSSA 297
Query: 986 TIEWVMAELIKNPDKMAKVR 1045
TIEWV+AELIK+P +MAKVR
Sbjct: 298 TIEWVIAELIKSPKEMAKVR 317
Score = 257 bits (657), Expect(2) = 0.0
Identities = 119/151 (78%), Positives = 133/151 (88%)
Frame = +1
Query: 1054 PQLPYLHACIKEAMRLHPAAPFLLSHRAVESCKIMGYTIPKDCKILVNAYAIGRDPKSWN 1233
PQLPYL ACIKEAMRLHPAAPFLL RA E+C++MGYTIPK+ ++LVNAYAIGRDPKSW
Sbjct: 335 PQLPYLQACIKEAMRLHPAAPFLLPRRAAETCEVMGYTIPKNSQVLVNAYAIGRDPKSWK 394
Query: 1234 DPTTFRPERFLESDVDYHGGHYKFIPFGSGRRICIGMPLATRTIPLIVASLVHAYDWRLP 1413
DP+TF PERFLESDVD+HG HY+FIPFGSGRR C+GMPLATRTIPLIV SLVH YD+ LP
Sbjct: 395 DPSTFWPERFLESDVDFHGAHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLP 454
Query: 1414 NGKHPQDLEMKEMLSLSLAIDPSLCVVPNLR 1506
G P+DL+M EMLSL+LAIDPSLCVVP R
Sbjct: 455 GGNRPEDLKMNEMLSLTLAIDPSLCVVPKAR 485
>gb|AJD20229.1| corytuberine synthase [Sinopodophyllum hexandrum]
Length = 483
Score = 402 bits (1034), Expect(2) = e-176
Identities = 200/318 (62%), Positives = 244/318 (76%)
Frame = +2
Query: 92 IQIATYCIIFAILVMLALASKTKHKNLPPGPSAWPLIGSLPMLFTNTQVPLHVTLTNLAA 271
+++ T + ++L + K + KNLPPGP WP+IG++P LF++ P+HV LTN+A
Sbjct: 1 MELTTALLYLIPTILLYILFKPRSKNLPPGPKPWPIIGNIPALFSSAY-PMHVALTNMAR 59
Query: 272 AHGPIMTLWLGTQPTVIASTPESAAEVLKTNDRVCSGRYIPMSFRLKHHIKHSLVWADCT 451
HGPIM LWLGTQPT +AST E+A E+LKT+DR SGR + MSFRLKHHIK+SLVWADC+
Sbjct: 60 THGPIMILWLGTQPTAVASTTEAAMEILKTHDRSFSGRAVRMSFRLKHHIKYSLVWADCS 119
Query: 452 DNWKLLRKIARTEIFSPKMLQTQSRVREEKAGELVDFLRSKQGQAVKLSTFVFGTLLNIL 631
+ WKLLRKI RTEIFSPKMLQ Q VRE+K ELV+FL+ K+GQ VK+S VFGTLLNIL
Sbjct: 120 EYWKLLRKIVRTEIFSPKMLQVQEHVREQKVAELVEFLKGKEGQVVKISQLVFGTLLNIL 179
Query: 632 GNAVYSEDVFVYSDDEGGKQGIQSMIKEMLMIGAEPNLAEFYPILEGLDLQGLKRKCDDR 811
GN ++S+DVF Y +G K +Q++IKEMLMIGA PNLAEF+PI E DLQGLK++C+ R
Sbjct: 180 GNVIFSKDVFRYVGADGDK--MQTLIKEMLMIGAAPNLAEFWPIFEEFDLQGLKKRCNQR 237
Query: 812 FVRVMKLWEGTVXXXXXXXXXXXXXDMLDVLLANDFNDAQINALFLETFGPGSETSSATI 991
F VMK+W+GTV DMLDVLL NDFNDAQINA+FLETFGPGSETSSAT
Sbjct: 238 FSEVMKMWKGTV-EERKVKRNEEPKDMLDVLLLNDFNDAQINAVFLETFGPGSETSSATS 296
Query: 992 EWVMAELIKNPDKMAKVR 1045
EW +AELIKNP +AKVR
Sbjct: 297 EWALAELIKNPHILAKVR 314
Score = 245 bits (625), Expect(2) = e-176
Identities = 112/151 (74%), Positives = 130/151 (86%)
Frame = +1
Query: 1054 PQLPYLHACIKEAMRLHPAAPFLLSHRAVESCKIMGYTIPKDCKILVNAYAIGRDPKSWN 1233
P LPYL ACIKE MRLHPAAPFLL HRAVE+C +MGYTIPKD ++LVN YAIGRDPK+W
Sbjct: 332 PHLPYLQACIKETMRLHPAAPFLLPHRAVETCPVMGYTIPKDTQVLVNIYAIGRDPKTWK 391
Query: 1234 DPTTFRPERFLESDVDYHGGHYKFIPFGSGRRICIGMPLATRTIPLIVASLVHAYDWRLP 1413
+PTTF+PERFLESDVDY+G H++FIPFG+GRR C+GMPLATRTIPLIV++LV DW LP
Sbjct: 392 EPTTFKPERFLESDVDYYGKHFEFIPFGAGRRQCVGMPLATRTIPLIVSNLVQTLDWSLP 451
Query: 1414 NGKHPQDLEMKEMLSLSLAIDPSLCVVPNLR 1506
+GK PQDL M E LSLSLA+DP+L V+P R
Sbjct: 452 DGKLPQDLVMNESLSLSLALDPTLTVIPKSR 482
>sp|P47195.1|C80A1_BERST RecName: Full=Berbamunine synthase; AltName:
Full=(S)-N-methylcoclaurine oxidase [C-O
phenol-coupling]; AltName: Full=CYPLXXX; AltName:
Full=Cytochrome P450 80 gi|642386|gb|AAC48987.1|
cytochrome P-450 CYP80 [Berberis stolonifera]
Length = 487
Score = 296 bits (757), Expect(2) = e-128
Identities = 150/316 (47%), Positives = 210/316 (66%)
Frame = +2
Query: 98 IATYCIIFAILVMLALASKTKHKNLPPGPSAWPLIGSLPMLFTNTQVPLHVTLTNLAAAH 277
I + I + ++ L K KH NLPP P AWP++G LP L + P ++N+A +
Sbjct: 4 IVGFVSISLVALLYFLLFKPKHTNLPPSPPAWPIVGHLPDLISKNSPPFLDYMSNIAQKY 63
Query: 278 GPIMTLWLGTQPTVIASTPESAAEVLKTNDRVCSGRYIPMSFRLKHHIKHSLVWADCTDN 457
GP++ L G ++ AST E+A EVL+TND+V SGR FR+K HI +S++W+D
Sbjct: 64 GPLIHLKFGLHSSIFASTKEAAMEVLQTNDKVLSGRQPLPCFRIKPHIDYSILWSDSNSY 123
Query: 458 WKLLRKIARTEIFSPKMLQTQSRVREEKAGELVDFLRSKQGQAVKLSTFVFGTLLNILGN 637
WK RKI TEIFS KMLQ Q + RE AG LV+F+ +K G V+L +++FG LN+LG+
Sbjct: 124 WKKGRKILHTEIFSQKMLQAQEKNRERVAGNLVNFIMTKVGDVVELRSWLFGCALNVLGH 183
Query: 638 AVYSEDVFVYSDDEGGKQGIQSMIKEMLMIGAEPNLAEFYPILEGLDLQGLKRKCDDRFV 817
V+S+DVF YS D+ + G+ +I MLM G + ++A ++P+L DL GLKRK D++F
Sbjct: 184 VVFSKDVFEYS-DQSDEVGMDKLIHGMLMTGGDFDVASYFPVLARFDLHGLKRKMDEQFK 242
Query: 818 RVMKLWEGTVXXXXXXXXXXXXXDMLDVLLANDFNDAQINALFLETFGPGSETSSATIEW 997
++K+WEG V DMLDVL+ANDFN+ QINA+F+ETFGPGS+T+S IEW
Sbjct: 243 LLIKIWEGEV-LARRANRNPEPKDMLDVLIANDFNEHQINAMFMETFGPGSDTNSNIIEW 301
Query: 998 VMAELIKNPDKMAKVR 1045
+A+LIKNPDK+AK+R
Sbjct: 302 ALAQLIKNPDKLAKLR 317
Score = 193 bits (490), Expect(2) = e-128
Identities = 85/150 (56%), Positives = 115/150 (76%)
Frame = +1
Query: 1057 QLPYLHACIKEAMRLHPAAPFLLSHRAVESCKIMGYTIPKDCKILVNAYAIGRDPKSWND 1236
+LPYL AC+KE MRL+P ++ HR +E+C++MGYTIPK + VNA+AIGRDPK W D
Sbjct: 337 ELPYLQACVKETMRLYPPISIMIPHRCMETCQVMGYTIPKGMDVHVNAHAIGRDPKDWKD 396
Query: 1237 PTTFRPERFLESDVDYHGGHYKFIPFGSGRRICIGMPLATRTIPLIVASLVHAYDWRLPN 1416
P F+PERFL+SD++Y+G ++FIPFGSGRRIC G PLA R IPL++ASLVHA+ W LP+
Sbjct: 397 PLKFQPERFLDSDIEYNGKQFQFIPFGSGRRICPGRPLAVRIIPLVLASLVHAFGWELPD 456
Query: 1417 GKHPQDLEMKEMLSLSLAIDPSLCVVPNLR 1506
G + L+M+E+ +LSL + L V+P +R
Sbjct: 457 GVPNEKLDMEELFTLSLCMAKPLRVIPKVR 486
>ref|XP_010253990.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nelumbo nucifera]
Length = 485
Score = 280 bits (715), Expect(2) = e-126
Identities = 143/312 (45%), Positives = 204/312 (65%), Gaps = 2/312 (0%)
Frame = +2
Query: 116 IFAILVMLA--LASKTKHKNLPPGPSAWPLIGSLPMLFTNTQVPLHVTLTNLAAAHGPIM 289
IF +L +L+ L K NLPPGP +WP+IG+L + HV L+ LA GP+M
Sbjct: 8 IFFLLSILSVVLFLKPSPNNLPPGPFSWPIIGTL---LPKLKKQPHVELSKLAQRFGPLM 64
Query: 290 TLWLGTQPTVIASTPESAAEVLKTNDRVCSGRYIPMSFRLKHHIKHSLVWADCTDNWKLL 469
L G +P V+AS+ +A EVLK DR+ SGR+ P S R+K +I+HS+VWADCTD WK++
Sbjct: 65 LLKFGVEPVVVASSHVAAVEVLKNQDRLLSGRFAPHSVRIKGYIEHSMVWADCTDYWKMV 124
Query: 470 RKIARTEIFSPKMLQTQSRVREEKAGELVDFLRSKQGQAVKLSTFVFGTLLNILGNAVYS 649
RK+ RTE+FS KML Q+ REEK EL+ FL K+G+ V + +FG++LNILG ++S
Sbjct: 125 RKVWRTELFSTKMLDIQAHAREEKVSELMKFLIRKEGEKVNFADVIFGSILNILGALIFS 184
Query: 650 EDVFVYSDDEGGKQGIQSMIKEMLMIGAEPNLAEFYPILEGLDLQGLKRKCDDRFVRVMK 829
+DV+ + D G++ MI++++++ A PNLA+ YPIL G D QGL++ R+ +
Sbjct: 185 KDVYDFEDRTDNNLGMKGMIRQLMILAAIPNLADLYPILGGSDFQGLRKASAACVKRMNE 244
Query: 830 LWEGTVXXXXXXXXXXXXXDMLDVLLANDFNDAQINALFLETFGPGSETSSATIEWVMAE 1009
W + D L VLL + F+D QI+A+ LETFGPGS+TS++TIEW MAE
Sbjct: 245 SW-AAIVKQRRKNDDHSKNDFLQVLLDSGFSDPQIDAMLLETFGPGSDTSTSTIEWAMAE 303
Query: 1010 LIKNPDKMAKVR 1045
L++NP+K+ KVR
Sbjct: 304 LLRNPEKLVKVR 315
Score = 201 bits (512), Expect(2) = e-126
Identities = 87/151 (57%), Positives = 115/151 (76%)
Frame = +1
Query: 1054 PQLPYLHACIKEAMRLHPAAPFLLSHRAVESCKIMGYTIPKDCKILVNAYAIGRDPKSWN 1233
P LPYLHAC+KE +RLHP FLL HRA+E+C++M YTIPK C+++VN YAIGRD K+W
Sbjct: 334 PNLPYLHACVKETLRLHPPVTFLLPHRAMETCQMMNYTIPKGCQLMVNTYAIGRDSKTWE 393
Query: 1234 DPTTFRPERFLESDVDYHGGHYKFIPFGSGRRICIGMPLATRTIPLIVASLVHAYDWRLP 1413
P +F PERFL S++DY G +++IPFG+GRRIC G+ LATR + LI+ASL+H +DW LP
Sbjct: 394 KPLSFLPERFLNSELDYQGNDFQYIPFGAGRRICPGLSLATRVVRLILASLLHTFDWSLP 453
Query: 1414 NGKHPQDLEMKEMLSLSLAIDPSLCVVPNLR 1506
+G HP +L+M + L+L D L V+P LR
Sbjct: 454 DGMHPDELDMNDKFGLALQKDIPLVVIPKLR 484
>gb|AGC29949.1| CYP80G3 [Sinopodophyllum hexandrum]
Length = 346
Score = 245 bits (625), Expect(2) = e-123
Identities = 112/151 (74%), Positives = 130/151 (86%)
Frame = +1
Query: 1054 PQLPYLHACIKEAMRLHPAAPFLLSHRAVESCKIMGYTIPKDCKILVNAYAIGRDPKSWN 1233
P LPYL ACIKE MRLHPAAPFLL HRAVE+C +MGYTIPKD ++LVN YAIGRDPK+W
Sbjct: 195 PHLPYLQACIKETMRLHPAAPFLLPHRAVETCPVMGYTIPKDTQVLVNIYAIGRDPKTWK 254
Query: 1234 DPTTFRPERFLESDVDYHGGHYKFIPFGSGRRICIGMPLATRTIPLIVASLVHAYDWRLP 1413
+PTTF+PERFLESDVDY+G H++FIPFG+GRR C+GMPLATRTIPLIV++LV DW LP
Sbjct: 255 EPTTFKPERFLESDVDYYGKHFEFIPFGAGRRQCVGMPLATRTIPLIVSNLVQTLDWSLP 314
Query: 1414 NGKHPQDLEMKEMLSLSLAIDPSLCVVPNLR 1506
+GK PQDL M E LSLSLA+DP+L V+P R
Sbjct: 315 DGKLPQDLVMNESLSLSLALDPTLTVIPKSR 345
Score = 227 bits (578), Expect(2) = e-123
Identities = 117/180 (65%), Positives = 138/180 (76%)
Frame = +2
Query: 506 MLQTQSRVREEKAGELVDFLRSKQGQAVKLSTFVFGTLLNILGNAVYSEDVFVYSDDEGG 685
MLQ Q VRE+K ELV+FL+ K+GQ VK+S VFGTLLNILGN ++S+DVF Y +G
Sbjct: 1 MLQVQEHVREQKVAELVEFLKGKEGQVVKISQLVFGTLLNILGNVIFSKDVFRYVGADGD 60
Query: 686 KQGIQSMIKEMLMIGAEPNLAEFYPILEGLDLQGLKRKCDDRFVRVMKLWEGTVXXXXXX 865
K +Q++IKEMLMIGA PNLAEF+PI E DLQGLK++C+ RF VMK+W+GTV
Sbjct: 61 K--MQTLIKEMLMIGAAPNLAEFWPIFEEFDLQGLKKRCNQRFSEVMKMWKGTVEERKVK 118
Query: 866 XXXXXXXDMLDVLLANDFNDAQINALFLETFGPGSETSSATIEWVMAELIKNPDKMAKVR 1045
DMLDVLL NDFNDAQINA+FLETFGPGSETSSAT EW +AELIKNP +AKVR
Sbjct: 119 RNEEPK-DMLDVLLLNDFNDAQINAVFLETFGPGSETSSATSEWALAELIKNPHILAKVR 177
>ref|XP_010253989.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nelumbo nucifera]
Length = 486
Score = 264 bits (674), Expect(2) = e-120
Identities = 136/316 (43%), Positives = 203/316 (64%), Gaps = 1/316 (0%)
Frame = +2
Query: 98 IATYCIIFAILVMLALASKTKHKNLPPGPSAWPLIGSLPMLFTNTQVPLHVTLTNLAAAH 277
+A + + ++ L L K K LPPGP +WP+IG+ L + T P ++ L LA +
Sbjct: 4 LALFILFLLSILSLVLFLKPSSKKLPPGPFSWPIIGT--QLPSPTMKP-NLELFKLAQRY 60
Query: 278 GPIMTLWLGTQPTVIASTPESAAEVLKTNDRVCSGRYIPMSFRLKHHIKHSLVWADCTDN 457
GP+M G + V+AS +A EVLK DRV SGR+ S R+K +I++S+VWADCTD
Sbjct: 61 GPLMLFRFGFENVVVASNHVAAMEVLKNQDRVLSGRFKANSVRVKGYIEYSMVWADCTDY 120
Query: 458 WKLLRKIARTEIFSPKMLQTQSRVREEKAGELVDFLRSKQGQAVKLSTFVFGTLLNILGN 637
WK++RKI RTE+FS KML + VREEK EL+ FLR K+G+ V +FG +LN+LG
Sbjct: 121 WKMVRKILRTELFSTKMLDVHAHVREEKVSELMKFLRRKEGEEVNFVDVIFGCILNMLGA 180
Query: 638 AVYSEDVFVYSDDEGGKQGIQSMIKEMLMIGAEPNLAEFYPI-LEGLDLQGLKRKCDDRF 814
+YS+DV+ + D G++ MI++++++ A PN+A+ YPI +G D QGL+++
Sbjct: 181 LIYSKDVYDFEDKTDINLGMKGMIRQLMILAATPNIADLYPIFFDGSDFQGLRKESAACV 240
Query: 815 VRVMKLWEGTVXXXXXXXXXXXXXDMLDVLLANDFNDAQINALFLETFGPGSETSSATIE 994
R+ + W + D+L VLL + F+D QI+A+FLETFGPGS+TS++TIE
Sbjct: 241 KRMSESW-AAIINERRKNNDHTKNDLLQVLLDSGFSDPQIDAIFLETFGPGSDTSASTIE 299
Query: 995 WVMAELIKNPDKMAKV 1042
W +AEL++NP+K+ K+
Sbjct: 300 WALAELLRNPEKLVKL 315
Score = 197 bits (501), Expect(2) = e-120
Identities = 84/151 (55%), Positives = 112/151 (74%)
Frame = +1
Query: 1054 PQLPYLHACIKEAMRLHPAAPFLLSHRAVESCKIMGYTIPKDCKILVNAYAIGRDPKSWN 1233
P LPYLHAC+KE +RLHP PFL+ H A+ESC++M YTIPK ++LVN YAIGRDP +W+
Sbjct: 335 PNLPYLHACVKETLRLHPPVPFLIPHIALESCEVMNYTIPKGSEVLVNLYAIGRDPTTWD 394
Query: 1234 DPTTFRPERFLESDVDYHGGHYKFIPFGSGRRICIGMPLATRTIPLIVASLVHAYDWRLP 1413
+P +F PERFL S+VDY G H+++IPFG+GRR+C GM L TR + LI+A+LVH +DW LP
Sbjct: 395 NPNSFLPERFLNSEVDYQGNHFQYIPFGAGRRMCPGMSLGTRVVRLILAALVHTFDWSLP 454
Query: 1414 NGKHPQDLEMKEMLSLSLAIDPSLCVVPNLR 1506
G H +L+M + + + L V+P LR
Sbjct: 455 GGMHQDELDMADRFGVGFQKETPLVVIPTLR 485
>gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase from Eschscholzia
californica [Expression vector pWCD2353]
Length = 490
Score = 260 bits (665), Expect(2) = e-114
Identities = 129/323 (39%), Positives = 198/323 (61%), Gaps = 5/323 (1%)
Frame = +2
Query: 92 IQIATYCIIFAIL--VMLALASKTKHKNLPPGPSAWPLIGSLPMLFTNTQVPLHVTLTNL 265
+++ T +I I+ ++ L + HKNLPPGP WP++G+L L H L
Sbjct: 1 MEVVTVALIAVIISSILYLLFGSSGHKNLPPGPKPWPIVGNLLQLGEKP----HAQFAEL 56
Query: 266 AAAHGPIMTLWLGTQPTVIASTPESAAEVLKTNDRVCSGRYIPMSFRLKHHIKHSLVWAD 445
A +G I TL +GT+ V+AST +A+E+LKT+DR+ S RY+ SFR+K H+++S+VW+D
Sbjct: 57 AQTYGDIFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSD 116
Query: 446 CTDNWKLLRKIARTEIFSPKMLQTQSRVREEKAGELVDFLRSKQGQAVKLSTFVFGTLLN 625
CT+ WK LRK+ RTE+F+ KM+++Q+ VRE+K E+V++L KQG+ VK+ +FGTL+N
Sbjct: 117 CTETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVN 176
Query: 626 ILGNAVYSEDVFVYSDDEGGKQGIQSMIKEMLMIGAEPNLAEFYPILEGLDLQGLKRKCD 805
I GN ++S+++F D G + + ML +G N A+++P+L DL G +++
Sbjct: 177 IFGNLIFSQNIFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVA 236
Query: 806 DRFVRVMKLWEGTV---XXXXXXXXXXXXXDMLDVLLANDFNDAQINALFLETFGPGSET 976
+ + +W + D +DV L + ND QINAL +E FG G+ET
Sbjct: 237 ECLKGIYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTET 296
Query: 977 SSATIEWVMAELIKNPDKMAKVR 1045
S++TIEW M EL KNP AK+R
Sbjct: 297 SASTIEWAMTELTKNPKITAKLR 319
Score = 181 bits (458), Expect(2) = e-114
Identities = 84/161 (52%), Positives = 107/161 (66%)
Frame = +1
Query: 1024 GQNGESTXXXPQLPYLHACIKEAMRLHPAAPFLLSHRAVESCKIMGYTIPKDCKILVNAY 1203
G+ P LPYL A +KE +RLHP P LL RA+E+C I+ YTIPKDC+I+VNA+
Sbjct: 327 GERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVNAW 386
Query: 1204 AIGRDPKSWNDPTTFRPERFLESDVDYHGGHYKFIPFGSGRRICIGMPLATRTIPLIVAS 1383
IGRDPK+W DP TF PERFL S VD+ G + IPFG+GRRIC G+P+A + I L+VA+
Sbjct: 387 GIGRDPKTWIDPLTFSPERFLNSSVDFRGNDFSLIPFGAGRRICPGLPIANQFIALLVAT 446
Query: 1384 LVHAYDWRLPNGKHPQDLEMKEMLSLSLAIDPSLCVVPNLR 1506
V DW LPNG L ++E L+L +P L +VP R
Sbjct: 447 FVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFIVPKSR 487
>sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
AltName: Full=Cytochrome P450 80B1
gi|3127027|gb|AAC39452.1| (S)-N-methylcoclaurine
3'-hydroxylase [Eschscholzia californica]
Length = 487
Score = 258 bits (660), Expect(2) = e-114
Identities = 129/320 (40%), Positives = 196/320 (61%), Gaps = 5/320 (1%)
Frame = +2
Query: 101 ATYCIIFAIL--VMLALASKTKHKNLPPGPSAWPLIGSLPMLFTNTQVPLHVTLTNLAAA 274
+T +I I+ ++ L + HKNLPPGP WP++G+L L H LA
Sbjct: 3 STVALIAVIISSILYLLFGGSGHKNLPPGPKPWPIVGNLLQLGEKP----HAQFAELAQT 58
Query: 275 HGPIMTLWLGTQPTVIASTPESAAEVLKTNDRVCSGRYIPMSFRLKHHIKHSLVWADCTD 454
+G I TL +GT+ V+AST +A+E+LKT+DR+ S RY+ SFR+K H+++S+VW+DCT+
Sbjct: 59 YGDIFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTE 118
Query: 455 NWKLLRKIARTEIFSPKMLQTQSRVREEKAGELVDFLRSKQGQAVKLSTFVFGTLLNILG 634
WK LRK+ RTE+F+ KM+++Q+ VRE+K E+V++L KQG+ VK+ +FGTL+NI G
Sbjct: 119 TWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFG 178
Query: 635 NAVYSEDVFVYSDDEGGKQGIQSMIKEMLMIGAEPNLAEFYPILEGLDLQGLKRKCDDRF 814
N ++S+++F D G + + ML +G N A+++P+L DL G +++ +
Sbjct: 179 NLIFSQNIFELGDPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVAECL 238
Query: 815 VRVMKLWEGTV---XXXXXXXXXXXXXDMLDVLLANDFNDAQINALFLETFGPGSETSSA 985
+ +W + D +DV L + ND QINAL +E FG G+ETS++
Sbjct: 239 KGIYAIWGAMLQERKLAKKVDGYKSKNDFVDVCLDSGLNDYQINALLMELFGAGTETSAS 298
Query: 986 TIEWVMAELIKNPDKMAKVR 1045
TIEW M EL KNP AK+R
Sbjct: 299 TIEWAMTELTKNPKITAKIR 318
Score = 182 bits (461), Expect(2) = e-114
Identities = 84/161 (52%), Positives = 107/161 (66%)
Frame = +1
Query: 1024 GQNGESTXXXPQLPYLHACIKEAMRLHPAAPFLLSHRAVESCKIMGYTIPKDCKILVNAY 1203
G+ P LPYL A +KE +RLHP P LL RA+E+C I+ YTIPKDC+I+VNA+
Sbjct: 326 GERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVNAW 385
Query: 1204 AIGRDPKSWNDPTTFRPERFLESDVDYHGGHYKFIPFGSGRRICIGMPLATRTIPLIVAS 1383
IGRDPK+W DP TF PERFL S VD+ G + IPFG+GRRIC G+P+A + I L+VA+
Sbjct: 386 GIGRDPKTWTDPLTFSPERFLNSSVDFRGNDFSLIPFGAGRRICPGLPIANQFIALLVAT 445
Query: 1384 LVHAYDWRLPNGKHPQDLEMKEMLSLSLAIDPSLCVVPNLR 1506
V DW LPNG L ++E L+L +P L +VP R
Sbjct: 446 FVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFIVPKSR 486
>sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2;
AltName: Full=Cytochrome P450 80B2
gi|3127029|gb|AAC39453.1| (S)-N-methylcoclaurine
3'-hydroxylase [Eschscholzia californica]
Length = 488
Score = 258 bits (658), Expect(2) = e-113
Identities = 128/323 (39%), Positives = 197/323 (60%), Gaps = 5/323 (1%)
Frame = +2
Query: 92 IQIATYCIIFAIL--VMLALASKTKHKNLPPGPSAWPLIGSLPMLFTNTQVPLHVTLTNL 265
+++ T +I I+ ++ L + HKNLPPGP WP++G+L L H L
Sbjct: 1 MEVVTVALIAVIISSILYLLFGSSGHKNLPPGPKPWPIVGNLLQLGEKP----HAQFAEL 56
Query: 266 AAAHGPIMTLWLGTQPTVIASTPESAAEVLKTNDRVCSGRYIPMSFRLKHHIKHSLVWAD 445
A +G I TL +GT+ V+AST +A+E+LKT+DR+ S RY+ SFR+K H+++S+VW+D
Sbjct: 57 AQTYGDIFTLKMGTETVVVASTSSAASEILKTHDRILSARYVFQSFRVKGHVENSIVWSD 116
Query: 446 CTDNWKLLRKIARTEIFSPKMLQTQSRVREEKAGELVDFLRSKQGQAVKLSTFVFGTLLN 625
CT+ WK LRK+ RTE+F+ KM+++Q+ VRE+K E+V++L KQG+ VK+ +FGTL+N
Sbjct: 117 CTETWKNLRKVCRTELFTQKMIESQAHVREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVN 176
Query: 626 ILGNAVYSEDVFVYSDDEGGKQGIQSMIKEMLMIGAEPNLAEFYPILEGLDLQGLKRKCD 805
I GN ++S+++F G + + ML +G N A+++P+L DL G +++
Sbjct: 177 IFGNLIFSQNIFELGXPNSGSSEFKEYLWRMLELGNSTNPADYFPMLGKFDLFGQRKEVA 236
Query: 806 DRFVRVMKLWEGTV---XXXXXXXXXXXXXDMLDVLLANDFNDAQINALFLETFGPGSET 976
+ + +W + D +DV L + ND QINAL +E FG G+ET
Sbjct: 237 ECLKGIYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLNDYQINALLMELFGAGTET 296
Query: 977 SSATIEWVMAELIKNPDKMAKVR 1045
S++TIEW M EL KNP AK+R
Sbjct: 297 SASTIEWAMTELTKNPKITAKLR 319
Score = 181 bits (458), Expect(2) = e-113
Identities = 84/161 (52%), Positives = 107/161 (66%)
Frame = +1
Query: 1024 GQNGESTXXXPQLPYLHACIKEAMRLHPAAPFLLSHRAVESCKIMGYTIPKDCKILVNAY 1203
G+ P LPYL A +KE +RLHP P LL RA+E+C I+ YTIPKDC+I+VNA+
Sbjct: 327 GERSVKESDFPNLPYLEATVKETLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVNAW 386
Query: 1204 AIGRDPKSWNDPTTFRPERFLESDVDYHGGHYKFIPFGSGRRICIGMPLATRTIPLIVAS 1383
IGRDPK+W DP TF PERFL S VD+ G + IPFG+GRRIC G+P+A + I L+VA+
Sbjct: 387 GIGRDPKTWIDPLTFSPERFLNSSVDFRGNDFSLIPFGAGRRICPGLPIANQFIALLVAT 446
Query: 1384 LVHAYDWRLPNGKHPQDLEMKEMLSLSLAIDPSLCVVPNLR 1506
V DW LPNG L ++E L+L +P L +VP R
Sbjct: 447 FVQNLDWCLPNGMSVDHLIVEEKFGLTLQKEPPLFIVPKSR 487
>gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum flavum subsp.
glaucum]
Length = 491
Score = 246 bits (629), Expect(2) = e-112
Identities = 125/323 (38%), Positives = 197/323 (60%)
Frame = +2
Query: 92 IQIATYCIIFAILVMLALASKTKHKNLPPGPSAWPLIGSLPMLFTNTQVPLHVTLTNLAA 271
+ A + F L + + +++K+LPPGP P++G+L L H LA
Sbjct: 4 LSAAMVSLFFIFLFIFFILCDSRNKDLPPGPRPSPIVGNLLQLGEKP----HAEFAKLAE 59
Query: 272 AHGPIMTLWLGTQPTVIASTPESAAEVLKTNDRVCSGRYIPMSFRLKHHIKHSLVWADCT 451
+G + TL LG+Q V+AS+P +AAE+LKT D++ SGRY+ SFR+ H+ +S+VW++C
Sbjct: 60 KYGELFTLKLGSQTVVVASSPAAAAEILKTRDKILSGRYVFQSFRVYEHVLNSIVWSECN 119
Query: 452 DNWKLLRKIARTEIFSPKMLQTQSRVREEKAGELVDFLRSKQGQAVKLSTFVFGTLLNIL 631
+NWKLLRK+ RTE+FSPKM+++Q+ +RE KA ++V FLR K+ Q VK+ VF TL+NI
Sbjct: 120 ENWKLLRKVCRTELFSPKMIESQAYIREAKALDMVRFLRKKENQEVKIVEVVFNTLVNIF 179
Query: 632 GNAVYSEDVFVYSDDEGGKQGIQSMIKEMLMIGAEPNLAEFYPILEGLDLQGLKRKCDDR 811
GN ++S+DVF D GG ++ + ++L +G N A+++PI+ DL G +R+
Sbjct: 180 GNLIFSKDVFDLEDPTGGSAELKEHLWKLLDMGNSTNPADYFPIMGKFDLFGQRREVAKV 239
Query: 812 FVRVMKLWEGTVXXXXXXXXXXXXXDMLDVLLANDFNDAQINALFLETFGPGSETSSATI 991
++ +W G + D +VLL +D +INAL +E FG G+ET+++TI
Sbjct: 240 LKQIYDVW-GVMLKERRSITGHRENDFANVLLNAGLDDQKINALLMELFGAGTETTASTI 298
Query: 992 EWVMAELIKNPDKMAKVRXXXLN 1060
EW + EL N ++K+R +N
Sbjct: 299 EWAITELTNNLRVISKLRAELIN 321
Score = 189 bits (480), Expect(2) = e-112
Identities = 85/151 (56%), Positives = 109/151 (72%)
Frame = +1
Query: 1054 PQLPYLHACIKEAMRLHPAAPFLLSHRAVESCKIMGYTIPKDCKILVNAYAIGRDPKSWN 1233
P LPYL A +KE +RLHP P LL RA+E+CK+M YTIPK+C+I+VNA+AIGRDPK+W+
Sbjct: 334 PHLPYLQAIVKETLRLHPPTPLLLPRRALETCKVMNYTIPKECQIMVNAWAIGRDPKTWD 393
Query: 1234 DPTTFRPERFLESDVDYHGGHYKFIPFGSGRRICIGMPLATRTIPLIVASLVHAYDWRLP 1413
DP TF+PERF+ S VDY G ++ IPFG GRRIC G+PLA++ + LIVA+LV +DW P
Sbjct: 394 DPLTFKPERFMNSTVDYKGNDFELIPFGGGRRICPGLPLASQFLSLIVATLVQNFDWSFP 453
Query: 1414 NGKHPQDLEMKEMLSLSLAIDPSLCVVPNLR 1506
G P ++ M E L L DP L +V R
Sbjct: 454 QGMTPNEVPMDEKFGLPLQKDPPLLIVLKAR 484
>gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [Papaver bracteatum]
Length = 486
Score = 248 bits (633), Expect(2) = e-112
Identities = 121/301 (40%), Positives = 187/301 (62%), Gaps = 2/301 (0%)
Frame = +2
Query: 164 KNLPPGPSAWPLIGSLPMLFTNTQVPLHVTLTNLAAAHGPIMTLWLGTQPTVIASTPESA 343
K LPPGP WP++G+L L H LA +G + TL LG++ V+ASTP +A
Sbjct: 26 KGLPPGPKPWPIVGNLLQLGEKP----HSQFAELAKTYGDLFTLKLGSETVVVASTPLAA 81
Query: 344 AEVLKTNDRVCSGRYIPMSFRLKHHIKHSLVWADCTDNWKLLRKIARTEIFSPKMLQTQS 523
+E+LKT+DRV SGRY+ SFR+K H+++S+VW++C + WK LRK+ R E+F+ KM+++Q+
Sbjct: 82 SEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRAELFTQKMIESQA 141
Query: 524 RVREEKAGELVDFLRSKQGQAVKLSTFVFGTLLNILGNAVYSEDVFVYSDDEGGKQGIQS 703
+RE KA E+V+FL+ QG VK+ VFGTL+NI GN ++S+++F D+ G ++
Sbjct: 142 EIRESKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKE 201
Query: 704 MIKEMLMIGAEPNLAEFYPILEGLDLQGLKRKCDDRFVRVMKLWEGTV--XXXXXXXXXX 877
+ ML +G N A+++P L DL G ++ D + +W +
Sbjct: 202 HLWRMLELGNSTNPADYFPFLGRFDLFGQRKDVADCLQGIYSVWGAMLKERKIAKLHNNS 261
Query: 878 XXXDMLDVLLANDFNDAQINALFLETFGPGSETSSATIEWVMAELIKNPDKMAKVRXXXL 1057
D +++LL + +D QINAL +E FG G+ETS++TIEW ++EL KNP+ A +R L
Sbjct: 262 KKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPEVTANMRSELL 321
Query: 1058 N 1060
+
Sbjct: 322 S 322
Score = 186 bits (472), Expect(2) = e-112
Identities = 84/151 (55%), Positives = 108/151 (71%)
Frame = +1
Query: 1054 PQLPYLHACIKEAMRLHPAAPFLLSHRAVESCKIMGYTIPKDCKILVNAYAIGRDPKSWN 1233
P +PYL A +KE +RLHPA P LL RA+E+CK++ YTIPK+C+I+VNA+ IGRDPK W
Sbjct: 335 PNMPYLQAFVKETLRLHPATPLLLPRRALETCKVLNYTIPKECQIMVNAWGIGRDPKRWT 394
Query: 1234 DPTTFRPERFLESDVDYHGGHYKFIPFGSGRRICIGMPLATRTIPLIVASLVHAYDWRLP 1413
DP F PERFL S +D+ G ++ IPFG+GRRIC G+PLAT+ I LIV +LV +DW LP
Sbjct: 395 DPLKFAPERFLNSSIDFKGNDFELIPFGAGRRICPGVPLATQFISLIVPTLVQNFDWGLP 454
Query: 1414 NGKHPQDLEMKEMLSLSLAIDPSLCVVPNLR 1506
G P L M+E L+L +P L +VP R
Sbjct: 455 KGMDPSQLIMEEKFGLTLQKEPPLYIVPKNR 485
>gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
Length = 481
Score = 244 bits (622), Expect(2) = e-111
Identities = 119/301 (39%), Positives = 186/301 (61%), Gaps = 2/301 (0%)
Frame = +2
Query: 164 KNLPPGPSAWPLIGSLPMLFTNTQVPLHVTLTNLAAAHGPIMTLWLGTQPTVIASTPESA 343
K LPPGP WP++G+L L H LA +G + +L LG++ V+ASTP +A
Sbjct: 21 KGLPPGPKPWPIVGNLLQLGEKP----HSQFAQLAETYGDLFSLKLGSETVVVASTPLAA 76
Query: 344 AEVLKTNDRVCSGRYIPMSFRLKHHIKHSLVWADCTDNWKLLRKIARTEIFSPKMLQTQS 523
+E+LKT+DRV SGRY+ SFR+K H+++S+VW++C + WK LRK+ RT++F+ KM+++Q+
Sbjct: 77 SEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTDLFTQKMIESQA 136
Query: 524 RVREEKAGELVDFLRSKQGQAVKLSTFVFGTLLNILGNAVYSEDVFVYSDDEGGKQGIQS 703
VRE KA E+V++L+ G VK++ VFGTL+NI GN ++S+++F D+ G ++
Sbjct: 137 EVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKE 196
Query: 704 MIKEMLMIGAEPNLAEFYPILEGLDLQGLKRKCDDRFVRVMKLWEGTV--XXXXXXXXXX 877
+ ML +G N A+++P L DL G + D + +W +
Sbjct: 197 HLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLKESKIAKQHNNS 256
Query: 878 XXXDMLDVLLANDFNDAQINALFLETFGPGSETSSATIEWVMAELIKNPDKMAKVRXXXL 1057
D +++LL + +D QINAL +E FG G+ETS++TIEW ++EL KNP A +R L
Sbjct: 257 KKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPQVTANMRLELL 316
Query: 1058 N 1060
+
Sbjct: 317 S 317
Score = 189 bits (479), Expect(2) = e-111
Identities = 85/151 (56%), Positives = 109/151 (72%)
Frame = +1
Query: 1054 PQLPYLHACIKEAMRLHPAAPFLLSHRAVESCKIMGYTIPKDCKILVNAYAIGRDPKSWN 1233
P +PYL A +KE +RLHPA P LL RA+E+CK++ YTIPK+C+I+VNA+ IGRDPK W
Sbjct: 330 PNMPYLQAFVKETLRLHPATPLLLPRRALETCKVLNYTIPKECQIMVNAWGIGRDPKRWT 389
Query: 1234 DPTTFRPERFLESDVDYHGGHYKFIPFGSGRRICIGMPLATRTIPLIVASLVHAYDWRLP 1413
DP F PERFL S +D+ G ++ IPFG+GRRIC G+PLAT+ I LIV+SLV +DW LP
Sbjct: 390 DPLKFSPERFLNSSIDFKGNDFELIPFGAGRRICPGVPLATQFISLIVSSLVQNFDWGLP 449
Query: 1414 NGKHPQDLEMKEMLSLSLAIDPSLCVVPNLR 1506
G P L M+E L+L +P L +VP R
Sbjct: 450 KGMDPSQLIMEEKFGLTLQKEPPLYIVPKTR 480
>emb|CBI34714.3| unnamed protein product [Vitis vinifera]
Length = 936
Score = 238 bits (606), Expect(2) = e-111
Identities = 129/316 (40%), Positives = 199/316 (62%), Gaps = 6/316 (1%)
Frame = +2
Query: 113 IIFAILVMLALASKTKHKNLPPGPSAWPLIGSLPMLFTNTQVPLHVTLTNLAAAHGPIMT 292
++F IL +L +S K LPPGPS WP++G+L L +P H++L + ++GP+++
Sbjct: 458 LVFLILKLLKDSSSLKSPPLPPGPSPWPILGNLLHL---GNMP-HISLARFSQSYGPLIS 513
Query: 293 LWLGTQPTVIASTPESAAEVLKTNDRVCSGRYIPMSFRLKHHI--KHSLVWA-DCTDNWK 463
L LG+Q V+AST +A E+LKT+DRV SGRY+P + K+ + SL WA +C D WK
Sbjct: 514 LRLGSQILVVASTSSAAMEILKTHDRVLSGRYVPHAVPAKNSEINRMSLGWAVECNDAWK 573
Query: 464 LLRKIARTEIFSPKMLQTQSRVREEKAGELVDFLRSKQGQAVKLSTFVFGTLLNILGNAV 643
LR + R E+FS K++++Q+ V E+K E+V F+ +K+G+ +K+ VF T+LN L +
Sbjct: 574 NLRTVCRAELFSTKVMESQAWVGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVL 633
Query: 644 YSEDVFVYSDD--EGGKQGIQSMIKEMLMIGAEPNLAEFYPILEGLDLQGLKRKCDDRFV 817
S D + DD +GG +G ++++M+M A PNL +FY I GLDLQGL +K +
Sbjct: 634 MSRDFISFEDDYKDGGMKG---LVRKMMMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIA 690
Query: 818 RVMKLWEGTV-XXXXXXXXXXXXXDMLDVLLANDFNDAQINALFLETFGPGSETSSATIE 994
R+ +WE + D L++L+ + ++D QIN LF+E G++TSS+T+E
Sbjct: 691 RICSMWESVIRERREGASDDPSKQDFLNILIRSGYSDDQINQLFMELLTAGADTSSSTLE 750
Query: 995 WVMAELIKNPDKMAKV 1042
W MAELIK+P+ M KV
Sbjct: 751 WAMAELIKSPESMKKV 766
Score = 194 bits (494), Expect(2) = e-111
Identities = 87/164 (53%), Positives = 115/164 (70%)
Frame = +1
Query: 1015 KEPGQNGESTXXXPQLPYLHACIKEAMRLHPAAPFLLSHRAVESCKIMGYTIPKDCKILV 1194
+E N P LPYL AC+KE +RLHP+AP LL RA SC++M YTIPKD +I V
Sbjct: 772 REISDNLPKASDLPHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQIWV 831
Query: 1195 NAYAIGRDPKSWNDPTTFRPERFLESDVDYHGGHYKFIPFGSGRRICIGMPLATRTIPLI 1374
NA+AIGRDP +W DP F+PERFL S VD+ G + +FIPFG+GRRIC G+P+A R +PLI
Sbjct: 832 NAWAIGRDPMNWEDPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLPLI 891
Query: 1375 VASLVHAYDWRLPNGKHPQDLEMKEMLSLSLAIDPSLCVVPNLR 1506
+ASL H +DW LPNG P +L+M + ++L + L ++P +R
Sbjct: 892 LASLTHFFDWSLPNGTTPDELDMNDKFGIALQKEQPLLIIPKVR 935
>ref|XP_002283461.2| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 540
Score = 238 bits (606), Expect(2) = e-111
Identities = 129/316 (40%), Positives = 199/316 (62%), Gaps = 6/316 (1%)
Frame = +2
Query: 113 IIFAILVMLALASKTKHKNLPPGPSAWPLIGSLPMLFTNTQVPLHVTLTNLAAAHGPIMT 292
++F IL +L +S K LPPGPS WP++G+L L +P H++L + ++GP+++
Sbjct: 62 LVFLILKLLKDSSSLKSPPLPPGPSPWPILGNLLHL---GNMP-HISLARFSQSYGPLIS 117
Query: 293 LWLGTQPTVIASTPESAAEVLKTNDRVCSGRYIPMSFRLKHHI--KHSLVWA-DCTDNWK 463
L LG+Q V+AST +A E+LKT+DRV SGRY+P + K+ + SL WA +C D WK
Sbjct: 118 LRLGSQILVVASTSSAAMEILKTHDRVLSGRYVPHAVPAKNSEINRMSLGWAVECNDAWK 177
Query: 464 LLRKIARTEIFSPKMLQTQSRVREEKAGELVDFLRSKQGQAVKLSTFVFGTLLNILGNAV 643
LR + R E+FS K++++Q+ V E+K E+V F+ +K+G+ +K+ VF T+LN L +
Sbjct: 178 NLRTVCRAELFSTKVMESQAWVGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVL 237
Query: 644 YSEDVFVYSDD--EGGKQGIQSMIKEMLMIGAEPNLAEFYPILEGLDLQGLKRKCDDRFV 817
S D + DD +GG +G ++++M+M A PNL +FY I GLDLQGL +K +
Sbjct: 238 MSRDFISFEDDYKDGGMKG---LVRKMMMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIA 294
Query: 818 RVMKLWEGTV-XXXXXXXXXXXXXDMLDVLLANDFNDAQINALFLETFGPGSETSSATIE 994
R+ +WE + D L++L+ + ++D QIN LF+E G++TSS+T+E
Sbjct: 295 RICSMWESVIRERREGASDDPSKQDFLNILIRSGYSDDQINQLFMELLTAGADTSSSTLE 354
Query: 995 WVMAELIKNPDKMAKV 1042
W MAELIK+P+ M KV
Sbjct: 355 WAMAELIKSPESMKKV 370
Score = 194 bits (494), Expect(2) = e-111
Identities = 87/164 (53%), Positives = 115/164 (70%)
Frame = +1
Query: 1015 KEPGQNGESTXXXPQLPYLHACIKEAMRLHPAAPFLLSHRAVESCKIMGYTIPKDCKILV 1194
+E N P LPYL AC+KE +RLHP+AP LL RA SC++M YTIPKD +I V
Sbjct: 376 REISDNLPKASDLPHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQIWV 435
Query: 1195 NAYAIGRDPKSWNDPTTFRPERFLESDVDYHGGHYKFIPFGSGRRICIGMPLATRTIPLI 1374
NA+AIGRDP +W DP F+PERFL S VD+ G + +FIPFG+GRRIC G+P+A R +PLI
Sbjct: 436 NAWAIGRDPMNWEDPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLPLI 495
Query: 1375 VASLVHAYDWRLPNGKHPQDLEMKEMLSLSLAIDPSLCVVPNLR 1506
+ASL H +DW LPNG P +L+M + ++L + L ++P +R
Sbjct: 496 LASLTHFFDWSLPNGTTPDELDMNDKFGIALQKEQPLLIIPKVR 539
>sp|Q9SP06.1|C80B3_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
AltName: Full=Cytochrome P450 80B1; AltName:
Full=Cytochrome P450 80B3
gi|6176562|gb|AAF05621.1|AF191772_1
(S)-N-methylcoclaurine 3'-hydroxylase [Papaver
somniferum]
Length = 481
Score = 245 bits (625), Expect(2) = e-111
Identities = 120/301 (39%), Positives = 186/301 (61%), Gaps = 2/301 (0%)
Frame = +2
Query: 164 KNLPPGPSAWPLIGSLPMLFTNTQVPLHVTLTNLAAAHGPIMTLWLGTQPTVIASTPESA 343
K LPPGP WP++G+L L H LA +G + +L LG++ V+ASTP +A
Sbjct: 21 KGLPPGPKPWPIVGNLLQLGEKP----HSQFAQLAETYGDLFSLKLGSETVVVASTPLAA 76
Query: 344 AEVLKTNDRVCSGRYIPMSFRLKHHIKHSLVWADCTDNWKLLRKIARTEIFSPKMLQTQS 523
+E+LKT+DRV SGRY+ SFR+K H+++S+VW++C + WK LRK+ RTE+F+ KM+++Q+
Sbjct: 77 SEILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTELFTQKMIESQA 136
Query: 524 RVREEKAGELVDFLRSKQGQAVKLSTFVFGTLLNILGNAVYSEDVFVYSDDEGGKQGIQS 703
VRE KA E+V++L+ G VK++ VFGTL+NI GN ++S+++F D+ G ++
Sbjct: 137 EVRESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSGSVEMKE 196
Query: 704 MIKEMLMIGAEPNLAEFYPILEGLDLQGLKRKCDDRFVRVMKLWEGTV--XXXXXXXXXX 877
+ ML +G N A+++P L DL G + D + +W +
Sbjct: 197 HLWRMLELGNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLKERKIAKQHNNS 256
Query: 878 XXXDMLDVLLANDFNDAQINALFLETFGPGSETSSATIEWVMAELIKNPDKMAKVRXXXL 1057
D +++LL + +D QINAL +E FG G+ETS++TIEW ++EL KNP A +R L
Sbjct: 257 KKNDFVEILLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPQVTANMRLELL 316
Query: 1058 N 1060
+
Sbjct: 317 S 317
Score = 187 bits (475), Expect(2) = e-111
Identities = 84/151 (55%), Positives = 108/151 (71%)
Frame = +1
Query: 1054 PQLPYLHACIKEAMRLHPAAPFLLSHRAVESCKIMGYTIPKDCKILVNAYAIGRDPKSWN 1233
P +PYL A +KE +RLHPA P LL RA+E+CK++ YTIPK+C+I+VNA+ IGRDPK W
Sbjct: 330 PNMPYLQAFVKETLRLHPATPLLLPRRALETCKVLNYTIPKECQIMVNAWGIGRDPKRWT 389
Query: 1234 DPTTFRPERFLESDVDYHGGHYKFIPFGSGRRICIGMPLATRTIPLIVASLVHAYDWRLP 1413
DP F PERFL S +D+ G ++ IPFG+GRRIC G+PLAT+ I LIV+SLV +DW P
Sbjct: 390 DPLKFSPERFLNSSIDFKGNDFELIPFGAGRRICPGVPLATQFISLIVSSLVQNFDWGFP 449
Query: 1414 NGKHPQDLEMKEMLSLSLAIDPSLCVVPNLR 1506
G P L M+E L+L +P L +VP R
Sbjct: 450 KGMDPSQLIMEEKFGLTLQKEPPLYIVPKTR 480
>ref|XP_009609252.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nicotiana tomentosiformis]
Length = 495
Score = 246 bits (628), Expect(2) = e-111
Identities = 134/313 (42%), Positives = 201/313 (64%), Gaps = 6/313 (1%)
Frame = +2
Query: 125 ILVMLALASKTKHKNLPPGPSAWPLIGSLPMLFTNTQVPLHVTLTNLAAAHGPIMTLWLG 304
I +L L +K LPPGP WP++G++ M ++P H+TLTN A +GP+M+L LG
Sbjct: 19 IFFLLKLCKSSKIPQLPPGPKPWPILGNIHM----GKMP-HITLTNFAKTYGPLMSLKLG 73
Query: 305 TQPTVIASTPESAAEVLKTNDRVCSGRYIPMSFRLKHHI--KHSLVW-ADCTDNWKLLRK 475
TQ V+ S+P A E+LKT+DR+ SGR++P +F K K S+ W ++C + W+ LR
Sbjct: 74 TQCLVVGSSPSVAIEILKTHDRILSGRHVPNAFPFKKSELEKSSIGWTSECNNGWRYLRT 133
Query: 476 IARTEIFSPKMLQTQSRVREEKAGELVDFLRSKQGQAVKLSTFVFGTLLNILGNAVYSED 655
+ RTE+FS K+L++Q+ +RE+K EL++FLRSK+GQ V L +F T+LN+L N + S+D
Sbjct: 134 LCRTELFSGKVLESQACLREKKVKELIEFLRSKEGQVVNLGELIFATVLNMLSNVMISQD 193
Query: 656 VFVYSDDEGGKQGIQSMIKEMLMIGAEPNLAEFYPILEGLDLQGLKRKCDDRFVRV-MKL 832
+ V + E G++S+I+E + +G+ PN+++FYPIL LDLQGL+RK ++ K
Sbjct: 194 I-VKLEKETEDGGMKSLIREAMEVGSTPNISDFYPILCKLDLQGLRRKSMVLMSKIRSKT 252
Query: 833 WEGTV--XXXXXXXXXXXXXDMLDVLLANDFNDAQINALFLETFGPGSETSSATIEWVMA 1006
WE V D L+ LL N F + I+ LF+E F G++T+++TIEW +A
Sbjct: 253 WERIVEERRKSKESGSSSQHDFLETLLNNGFTNDAIHQLFMELFTAGTDTTTSTIEWAIA 312
Query: 1007 ELIKNPDKMAKVR 1045
ELIKN + M KVR
Sbjct: 313 ELIKNEESMEKVR 325
Score = 186 bits (471), Expect(2) = e-111
Identities = 78/147 (53%), Positives = 111/147 (75%)
Frame = +1
Query: 1057 QLPYLHACIKEAMRLHPAAPFLLSHRAVESCKIMGYTIPKDCKILVNAYAIGRDPKSWND 1236
Q+ Y+ AC+KE +RLHP PFLL HRA+E+C++M YTIPKD ++LVN +AIGRD W +
Sbjct: 345 QMSYIQACVKETLRLHPPGPFLLPHRAIETCQVMSYTIPKDAQVLVNVWAIGRDSLIWKE 404
Query: 1237 PTTFRPERFLESDVDYHGGHYKFIPFGSGRRICIGMPLATRTIPLIVASLVHAYDWRLPN 1416
P FRP+RFL S++D+ G ++F+PFGSGRRIC G+P+A +++PL++ASLVH +DW LPN
Sbjct: 405 PEMFRPQRFLSSNMDFKGNDFEFLPFGSGRRICPGLPMAAKSVPLVLASLVHFFDWELPN 464
Query: 1417 GKHPQDLEMKEMLSLSLAIDPSLCVVP 1497
GK +L+M E ++L L ++P
Sbjct: 465 GKCSIELDMNEKFGVTLQKREPLLLIP 491
>ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
communis] gi|223547255|gb|EEF48750.1|
(S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
[Ricinus communis]
Length = 496
Score = 239 bits (611), Expect(2) = e-111
Identities = 125/315 (39%), Positives = 200/315 (63%), Gaps = 5/315 (1%)
Frame = +2
Query: 116 IFAILVMLALAS-KTKHKNLPPGPSAWPLIGSLPMLFTNTQVPLHVTLTNLAAAHGPIMT 292
+ I+VM L+S +K + LPPGP WP++G++ + N H+++ N A HGP+++
Sbjct: 18 LILIIVMQILSSISSKRRPLPPGPRQWPILGNILQVGKNP----HISMANYAKVHGPLIS 73
Query: 293 LWLGTQPTVIASTPESAAEVLKTNDRVCSGRYIPMSFRLKHHI--KHSLVW-ADCTDNWK 463
L LGT+ V+AS+P +AAE+LKT+DR+ SGRYIP + + ++ + +LVW C+D WK
Sbjct: 74 LRLGTRVVVVASSPTAAAEILKTHDRLLSGRYIPATTPYEDNVLDRIALVWNPSCSDQWK 133
Query: 464 LLRKIARTEIFSPKMLQTQSRVREEKAGELVDFLRSKQGQAVKLSTFVFGTLLNILGNAV 643
LR + R+E+FS K +++Q+ +RE+K E++DFL SKQGQ V + VF T N + N +
Sbjct: 134 FLRAMCRSELFSAKAIESQATLREKKLTEMLDFLTSKQGQIVNIGEVVFTTAFNTISNLL 193
Query: 644 YSEDVFVYSDDEGGKQGIQSMIKEMLMIGAEPNLAEFYPILEGLDLQGLKRKCDDRFVRV 823
+S+D+ + +D+G ++++I ++ + PN+A+FYP+L LD QG+KRK + R+
Sbjct: 194 FSKDLLSF-EDQGNAGELKTLISTLMELATCPNIADFYPVLTKLDPQGIKRKMKNCLERM 252
Query: 824 MKLWEGTV-XXXXXXXXXXXXXDMLDVLLANDFNDAQINALFLETFGPGSETSSATIEWV 1000
+W+ + D LDV L+N F+D QIN L LE F G++T++ T+EW
Sbjct: 253 FGVWDIYIKERRERHVKDARKTDFLDVFLSNGFDDHQINWLLLELFSAGADTTTTTVEWA 312
Query: 1001 MAELIKNPDKMAKVR 1045
MAEL+K + KVR
Sbjct: 313 MAELLKEITVLEKVR 327
Score = 191 bits (486), Expect(2) = e-111
Identities = 83/148 (56%), Positives = 114/148 (77%)
Frame = +1
Query: 1054 PQLPYLHACIKEAMRLHPAAPFLLSHRAVESCKIMGYTIPKDCKILVNAYAIGRDPKSWN 1233
PQL YL+AC+KE +RLHP PFL+ RA E+C++M YTIPK +I+VN +AIGRDP +W
Sbjct: 345 PQLKYLNACVKETLRLHPPVPFLIPRRAPEACEVMNYTIPKHSQIIVNVWAIGRDPSAWE 404
Query: 1234 DPTTFRPERFLESDVDYHGGHYKFIPFGSGRRICIGMPLATRTIPLIVASLVHAYDWRLP 1413
DP ++RPERFL+S++D+ G +++F+PFGSGRRIC G+P+ TR +PLI+ASLVH +DW L
Sbjct: 405 DPLSYRPERFLDSNLDFKGHNFEFLPFGSGRRICPGLPMGTRQLPLILASLVHCFDWSLQ 464
Query: 1414 NGKHPQDLEMKEMLSLSLAIDPSLCVVP 1497
NG P L+M + S++L + L VVP
Sbjct: 465 NGDDPAMLDMNDKFSITLEKEQHLLVVP 492
>emb|CBI31830.3| unnamed protein product [Vitis vinifera]
Length = 1538
Score = 241 bits (615), Expect(2) = e-110
Identities = 137/325 (42%), Positives = 196/325 (60%), Gaps = 6/325 (1%)
Frame = +2
Query: 89 DIQIATYCIIFAILVMLALASKT--KHKNLPPGPSAWPLIGSLPMLFTNTQVPLHVTLTN 262
DI ++++ +L L + K LPPGP WP+IG+L L N HV L +
Sbjct: 1054 DISLSSFVYALLLLPFLLILKHIFLKPPPLPPGPYPWPIIGNLLQLGKNP----HVKLAS 1109
Query: 263 LAAAHGPIMTLWLGTQPTVIASTPESAAEVLKTNDRVCSGRYIPMSFRLKH-HIKH-SLV 436
LA HGP+M+L LGTQ V+AS+P +A EVLKT+DR SGRY+ S +K + H SL
Sbjct: 1110 LAKLHGPLMSLRLGTQLMVVASSPAAALEVLKTHDRTLSGRYVSSSLSVKDPKLNHLSLA 1169
Query: 437 WA-DCTDNWKLLRKIARTEIFSPKMLQTQSRVREEKAGELVDFLRSKQGQAVKLSTFVFG 613
+A +CT+NWK LR I RTE+FS K +++ +RE K ELV+FL +K+G+ VK+ VF
Sbjct: 1170 FAKECTNNWKNLRTICRTEMFSGKAMESHVELRERKVMELVEFLATKEGEVVKVMDLVFT 1229
Query: 614 TLLNILGNAVYSEDVFVYSDDEGGKQGIQSMIKEMLMIGAEPNLAEFYPILEGLDLQGLK 793
T+ NIL N +S D+ + ++G++ I +GA PNL++FYPIL+GL+L G K
Sbjct: 1230 TICNILSNTFFSMDLCDFE-----REGLKDFIYRAAELGATPNLSDFYPILDGLNLHGSK 1284
Query: 794 RKCDDRFVRVMKLWEGTV-XXXXXXXXXXXXXDMLDVLLANDFNDAQINALFLETFGPGS 970
+K + R++ WEGT+ D+L+ L F D QIN + LE F G+
Sbjct: 1285 KKSKEALGRILATWEGTLKERRKQKNPGSSHRDLLEAFLEIRFEDDQINQVILELFSAGA 1344
Query: 971 ETSSATIEWVMAELIKNPDKMAKVR 1045
+TS+ TIEW + +LI+NPD M K+R
Sbjct: 1345 DTSTLTIEWAITQLIRNPDVMYKLR 1369
Score = 187 bits (475), Expect(2) = e-110
Identities = 80/151 (52%), Positives = 114/151 (75%)
Frame = +1
Query: 1054 PQLPYLHACIKEAMRLHPAAPFLLSHRAVESCKIMGYTIPKDCKILVNAYAIGRDPKSWN 1233
P LPYL AC+KE +RLHP AP LL HRA+E+C++MGYTIPKD ++ VN +A+GRDPK W+
Sbjct: 1387 PHLPYLQACVKETLRLHPPAPLLLPHRAMETCQVMGYTIPKDSQVFVNIWAMGRDPKVWD 1446
Query: 1234 DPTTFRPERFLESDVDYHGGHYKFIPFGSGRRICIGMPLATRTIPLIVASLVHAYDWRLP 1413
DP +F PERFL+S +++ G +++IPFG+GRRIC GM L R +PL++A+LVH +DW LP
Sbjct: 1447 DPLSFTPERFLDSKLEFKGNDFEYIPFGAGRRICPGMALGARQVPLVLATLVHLFDWSLP 1506
Query: 1414 NGKHPQDLEMKEMLSLSLAIDPSLCVVPNLR 1506
+ ++M+E L ++L + L +VP +R
Sbjct: 1507 DNMDSAQIDMEEWLVITLRKENPLRLVPKVR 1537
Score = 244 bits (622), Expect(2) = e-109
Identities = 140/325 (43%), Positives = 192/325 (59%), Gaps = 6/325 (1%)
Frame = +2
Query: 89 DIQIATYCIIFAILVMLALASKT--KHKNLPPGPSAWPLIGSLPMLFTNTQVPLHVTLTN 262
DI I ++ +L L + K LPPGP WP+IG+L + N H L N
Sbjct: 549 DISIFSFLYALLLLPFLVILKHIFLKPPPLPPGPYPWPIIGNLLQMGKNP----HAKLAN 604
Query: 263 LAAAHGPIMTLWLGTQPTVIASTPESAAEVLKTNDRVCSGRYIPMSFRLKH-HIKH-SLV 436
LA HGP+M+L LGTQ V+AS+P +A EVLKT+DR SGRY+ S +K+ + H S+V
Sbjct: 605 LAKLHGPLMSLRLGTQLMVVASSPAAAMEVLKTHDRALSGRYLSSSVPVKNPKLNHLSIV 664
Query: 437 WA-DCTDNWKLLRKIARTEIFSPKMLQTQSRVREEKAGELVDFLRSKQGQAVKLSTFVFG 613
+A DC NWK LR I R E+FS K +++Q +RE K ELV+FL +K+G+ VK+ VF
Sbjct: 665 FAKDCNTNWKNLRAICRMELFSGKAMESQVELRERKVTELVEFLATKEGEVVKVMDLVFT 724
Query: 614 TLLNILGNAVYSEDVFVYSDDEGGKQGIQSMIKEMLMIGAEPNLAEFYPILEGLDLQGLK 793
T+ NIL N +S D+ + D+ ++ +I + GA PNL+++YPIL GLD+QG+
Sbjct: 725 TICNILSNKFFSMDLCDFEDEGRVGGALKDLIHKNAEFGATPNLSDYYPILGGLDIQGIN 784
Query: 794 RKCDDRFVRVMKLWEGTV-XXXXXXXXXXXXXDMLDVLLANDFNDAQINALFLETFGPGS 970
RK + F R+ WE + D L+ LL F D QIN + LE F G+
Sbjct: 785 RKAKEMFERIPTTWEDILKERRTQRSNRSSHRDFLEALLEIGFEDDQINQVILELFSAGA 844
Query: 971 ETSSATIEWVMAELIKNPDKMAKVR 1045
ETSS T+EW MAELI+N D M K+R
Sbjct: 845 ETSSLTVEWAMAELIRNQDAMDKLR 869
Score = 182 bits (462), Expect(2) = e-109
Identities = 81/156 (51%), Positives = 113/156 (72%)
Frame = +1
Query: 1054 PQLPYLHACIKEAMRLHPAAPFLLSHRAVESCKIMGYTIPKDCKILVNAYAIGRDPKSWN 1233
P+LPYL AC+KEA+RLHP AP LL H A E+C++MGYTIPKD +I VN +A+ RDPK W+
Sbjct: 887 PRLPYLQACVKEALRLHPPAPLLLPHLAAETCQVMGYTIPKDSQIFVNIWAMARDPKIWD 946
Query: 1234 DPTTFRPERFLESDVDYHGGHYKFIPFGSGRRICIGMPLATRTIPLIVASLVHAYDWRLP 1413
DP +F+PERFL+S +D+ G +++IPFG+GRRIC G+ L R +PLI+A+ VH + W LP
Sbjct: 947 DPLSFKPERFLDSKLDFKGNDFEYIPFGAGRRICPGLALGGRQVPLILATFVHLFGWSLP 1006
Query: 1414 NGKHPQDLEMKEMLSLSLAIDPSLCVVPNLRG*IIY 1521
L+M+E L ++L + L +VP +R I+
Sbjct: 1007 GNMDSAQLDMEEWLVITLRKEQPLRLVPRVRKYFIF 1042
Score = 216 bits (549), Expect(2) = 3e-98
Identities = 130/304 (42%), Positives = 180/304 (59%), Gaps = 9/304 (2%)
Frame = +2
Query: 158 KHKNLPPGPSAWPLIGSLPMLFTNTQVPLHVTLTNLAAAHGPIMTLWLGTQPTVIASTPE 337
K LPPGP WP+IG+L + N LHV L NLA HGP+M+L LGTQ V+AS+
Sbjct: 34 KSPPLPPGPYPWPIIGNLLQMGGN----LHVKLANLAKRHGPLMSLRLGTQIMVVASSSA 89
Query: 338 SAAEVLKTNDRVCSGRY----IPMSFRLKHHIKHSLVWADCTDNWKLLRKIARTEIFSPK 505
+A EVLKT+DR SGRY IP++ +H+ + C +W+ L+ I R E+FS K
Sbjct: 90 AAMEVLKTHDRTLSGRYVSTTIPVNSPKLNHLAMAFAKV-CNSDWRNLKAICRMELFSGK 148
Query: 506 MLQTQSRVREEKAGELVDFLRSKQGQAVKLSTFVFGTLLNILGNAVYSEDVFVYSDDEG- 682
++++ +RE K ELV+FL K+G+ VK+ V+ T+ NIL N +S D +SD EG
Sbjct: 149 AMESRVELRERKVMELVEFLEKKEGEVVKVMDLVYTTVCNILSNKFFSID---FSDFEGR 205
Query: 683 GKQGIQSMIKEMLMIGAE---PNLAEFYPILEGLDLQGLKRKCDDRFVRVMKLWEGTV-X 850
+G+ ++K++L AE N+ +FYPIL GLD+QG+++K + F R+ WE +
Sbjct: 206 DVRGV--LLKDLLNENAELGATNILDFYPILGGLDIQGIRKKLKEIFRRIPTTWEDILKE 263
Query: 851 XXXXXXXXXXXXDMLDVLLANDFNDAQINALFLETFGPGSETSSATIEWVMAELIKNPDK 1030
D LD LL F D QIN + +E F G ETSS T+EW MAELIKN D
Sbjct: 264 RRKQRIHGSSHGDFLDALLETGFEDDQINHVIMELFFAGPETSSLTVEWAMAELIKNQDA 323
Query: 1031 MAKV 1042
M K+
Sbjct: 324 MHKL 327
Score = 172 bits (437), Expect(2) = 3e-98
Identities = 73/138 (52%), Positives = 102/138 (73%)
Frame = +1
Query: 1054 PQLPYLHACIKEAMRLHPAAPFLLSHRAVESCKIMGYTIPKDCKILVNAYAIGRDPKSWN 1233
P LPYL AC+KE +RLHP P LL HRA E+C+IMGYTIPKD I VN +A+GRDP +W
Sbjct: 346 PHLPYLQACVKETLRLHPTGPLLLPHRATETCQIMGYTIPKDSIIFVNMWAMGRDPGTWE 405
Query: 1234 DPTTFRPERFLESDVDYHGGHYKFIPFGSGRRICIGMPLATRTIPLIVASLVHAYDWRLP 1413
DP +F+PERFL+S +++ G +++IPFG+GRR+C GMPLA R +P+I+A+ V +DW P
Sbjct: 406 DPLSFKPERFLDSKLEFKGNDFEYIPFGAGRRMCPGMPLAARLVPMILATFVRLFDWSTP 465
Query: 1414 NGKHPQDLEMKEMLSLSL 1467
+++M+E ++L
Sbjct: 466 GDMDFAEIDMEERFVITL 483
>ref|XP_002283415.2| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 540
Score = 234 bits (597), Expect(2) = e-110
Identities = 126/314 (40%), Positives = 198/314 (63%), Gaps = 4/314 (1%)
Frame = +2
Query: 113 IIFAILVMLALASKTKHKNLPPGPSAWPLIGSLPMLFTNTQVPLHVTLTNLAAAHGPIMT 292
++F IL +L +S K LPPGPS WP++G+L L +P H++L + ++GP+++
Sbjct: 62 LVFLILKLLKDSSSLKSPPLPPGPSPWPILGNLLHL---GNMP-HISLARFSQSYGPLIS 117
Query: 293 LWLGTQPTVIASTPESAAEVLKTNDRVCSGRYIPMSFRLKHHIKH--SLVWA-DCTDNWK 463
L LG+Q V+AST +A E+LKT+DRV SGRY+P + K+ + SL WA +C WK
Sbjct: 118 LRLGSQILVVASTSSAAMEILKTHDRVLSGRYVPHAVPAKNSEINPMSLGWAVECNGAWK 177
Query: 464 LLRKIARTEIFSPKMLQTQSRVREEKAGELVDFLRSKQGQAVKLSTFVFGTLLNILGNAV 643
LR + R E+FS K++++Q+ V E+K E+V F+ +K+G+ +K+ VF T+LN L +
Sbjct: 178 NLRTVCRAELFSTKVMESQAWVGEKKVMEMVRFVSTKEGEVMKVGEVVFATVLNTLTTVL 237
Query: 644 YSEDVFVYSDDEGGKQGIQSMIKEMLMIGAEPNLAEFYPILEGLDLQGLKRKCDDRFVRV 823
S D F+ +D+ G++ ++++M+M A PNL +FY I GLDLQGL +K + R+
Sbjct: 238 MSRD-FISFEDDNKDGGMKGLVRKMVMAMAAPNLDDFYLIFSGLDLQGLNKKTKELIARI 296
Query: 824 MKLWEGTV-XXXXXXXXXXXXXDMLDVLLANDFNDAQINALFLETFGPGSETSSATIEWV 1000
+WE + D L++L+ + ++D QIN LF+E G++TSS+T+EW
Sbjct: 297 CSMWESVIRERREGASDDPSKQDFLNILIRSGYSDDQINQLFMELLTAGADTSSSTLEWA 356
Query: 1001 MAELIKNPDKMAKV 1042
MAELIK+P+ M KV
Sbjct: 357 MAELIKSPESMKKV 370
Score = 194 bits (493), Expect(2) = e-110
Identities = 87/164 (53%), Positives = 115/164 (70%)
Frame = +1
Query: 1015 KEPGQNGESTXXXPQLPYLHACIKEAMRLHPAAPFLLSHRAVESCKIMGYTIPKDCKILV 1194
+E N P LPYL AC+KE +RLHP+AP LL RA SC++M YTIPKD +I V
Sbjct: 376 REISDNLPKASDLPHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQIWV 435
Query: 1195 NAYAIGRDPKSWNDPTTFRPERFLESDVDYHGGHYKFIPFGSGRRICIGMPLATRTIPLI 1374
NA+AIGRDP +W DP F+PERFL S VD+ G + +FIPFG+GRRIC G+P+A R +PLI
Sbjct: 436 NAWAIGRDPMNWEDPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLPLI 495
Query: 1375 VASLVHAYDWRLPNGKHPQDLEMKEMLSLSLAIDPSLCVVPNLR 1506
+ASL H +DW LPNG P +L+M + ++L + L ++P +R
Sbjct: 496 LASLTHFFDWSLPNGTTPDELDMNDKFGVTLQKEQPLLIIPKVR 539
>ref|XP_010662515.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 493
Score = 241 bits (615), Expect(2) = e-110
Identities = 137/325 (42%), Positives = 196/325 (60%), Gaps = 6/325 (1%)
Frame = +2
Query: 89 DIQIATYCIIFAILVMLALASKT--KHKNLPPGPSAWPLIGSLPMLFTNTQVPLHVTLTN 262
DI ++++ +L L + K LPPGP WP+IG+L L N HV L +
Sbjct: 9 DISLSSFVYALLLLPFLLILKHIFLKPPPLPPGPYPWPIIGNLLQLGKNP----HVKLAS 64
Query: 263 LAAAHGPIMTLWLGTQPTVIASTPESAAEVLKTNDRVCSGRYIPMSFRLKH-HIKH-SLV 436
LA HGP+M+L LGTQ V+AS+P +A EVLKT+DR SGRY+ S +K + H SL
Sbjct: 65 LAKLHGPLMSLRLGTQLMVVASSPAAALEVLKTHDRTLSGRYVSSSLSVKDPKLNHLSLA 124
Query: 437 WA-DCTDNWKLLRKIARTEIFSPKMLQTQSRVREEKAGELVDFLRSKQGQAVKLSTFVFG 613
+A +CT+NWK LR I RTE+FS K +++ +RE K ELV+FL +K+G+ VK+ VF
Sbjct: 125 FAKECTNNWKNLRTICRTEMFSGKAMESHVELRERKVMELVEFLATKEGEVVKVMDLVFT 184
Query: 614 TLLNILGNAVYSEDVFVYSDDEGGKQGIQSMIKEMLMIGAEPNLAEFYPILEGLDLQGLK 793
T+ NIL N +S D+ + ++G++ I +GA PNL++FYPIL+GL+L G K
Sbjct: 185 TICNILSNTFFSMDLCDFE-----REGLKDFIYRAAELGATPNLSDFYPILDGLNLHGSK 239
Query: 794 RKCDDRFVRVMKLWEGTV-XXXXXXXXXXXXXDMLDVLLANDFNDAQINALFLETFGPGS 970
+K + R++ WEGT+ D+L+ L F D QIN + LE F G+
Sbjct: 240 KKSKEALGRILATWEGTLKERRKQKNPGSSHRDLLEAFLEIRFEDDQINQVILELFSAGA 299
Query: 971 ETSSATIEWVMAELIKNPDKMAKVR 1045
+TS+ TIEW + +LI+NPD M K+R
Sbjct: 300 DTSTLTIEWAITQLIRNPDVMYKLR 324
Score = 187 bits (475), Expect(2) = e-110
Identities = 80/151 (52%), Positives = 114/151 (75%)
Frame = +1
Query: 1054 PQLPYLHACIKEAMRLHPAAPFLLSHRAVESCKIMGYTIPKDCKILVNAYAIGRDPKSWN 1233
P LPYL AC+KE +RLHP AP LL HRA+E+C++MGYTIPKD ++ VN +A+GRDPK W+
Sbjct: 342 PHLPYLQACVKETLRLHPPAPLLLPHRAMETCQVMGYTIPKDSQVFVNIWAMGRDPKVWD 401
Query: 1234 DPTTFRPERFLESDVDYHGGHYKFIPFGSGRRICIGMPLATRTIPLIVASLVHAYDWRLP 1413
DP +F PERFL+S +++ G +++IPFG+GRRIC GM L R +PL++A+LVH +DW LP
Sbjct: 402 DPLSFTPERFLDSKLEFKGNDFEYIPFGAGRRICPGMALGARQVPLVLATLVHLFDWSLP 461
Query: 1414 NGKHPQDLEMKEMLSLSLAIDPSLCVVPNLR 1506
+ ++M+E L ++L + L +VP +R
Sbjct: 462 DNMDSAQIDMEEWLVITLRKENPLRLVPKVR 492