BLASTX nr result
ID: Aconitum23_contig00022061
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Aconitum23_contig00022061
(661 letters)
Database: ./nr
77,306,371 sequences; 28,104,191,420 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-meth... 298 1e-78
gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictr... 293 5e-77
gb|ACO90250.1| norcoclaurine 6-O-methyltransferase-like protein ... 277 3e-72
gb|AKO60153.1| S-adenosyl-L-methionine:O-methyltransferase [Papa... 261 3e-67
dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica] 260 4e-67
ref|XP_010244054.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltran... 259 1e-66
gb|AJD20222.1| norcoclaurine 6-O-methyltransferase [Sinopodophyl... 253 5e-65
ref|XP_010247901.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltran... 251 2e-64
gb|AKH61490.1| 6-O-methyltransferase [Expression vector pWCD2353] 236 7e-60
sp|Q6WUC1.1|6OMT_PAPSO RecName: Full=(RS)-norcoclaurine 6-O-meth... 236 7e-60
gb|AKN62837.1| norcoclaurine 6-O-methyltransferase, partial [Pap... 234 4e-59
gb|AKN62836.1| norcoclaurine 6-O-methyltransferase, partial [Pap... 234 4e-59
gb|ACO90232.1| putative norcoclaurine 6-O-methyltransferase [Pap... 234 4e-59
gb|AAP45315.1| S-adenosyl-L-methionine:norcoclaurine 6-O-methylt... 233 8e-59
ref|XP_010271691.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltran... 231 3e-58
gb|AKO60152.1| S-adenosyl-L-methionine:O-methyltransferase [Papa... 226 1e-56
ref|XP_010247902.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltran... 224 5e-56
gb|AKH61488.1| 4' O-methyltransferase [Expression vector pWCD2353] 218 3e-54
gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltra... 218 3e-54
sp|Q7XB10.1|4OMT2_PAPSO RecName: Full=3'-hydroxy-N-methyl-(S)-co... 218 3e-54
>sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-methyltransferase; AltName:
Full=S-adenosyl-L-methionine:norcoclaurine
6-O-methyltransferase; Short=6-OMT
gi|9711914|dbj|BAB08004.1|
S-adenosyl-L-methionine:norcoclaurine
6-O-methyltransferase [Coptis japonica]
Length = 347
Score = 298 bits (764), Expect = 1e-78
Identities = 150/220 (68%), Positives = 170/220 (77%), Gaps = 1/220 (0%)
Frame = +3
Query: 3 ITDKDYMAPWHYLKDELSTENDVTAFEKALGMSPWGYLAANPEKSQFFNEAMANDCSLIM 182
ITDKD+MAPWHYLKD LS E+ TAFEKALG + WGY+A +PEK+Q FNEAMAND LIM
Sbjct: 113 ITDKDFMAPWHYLKDGLSGESG-TAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLIM 171
Query: 183 SALVRECSGSFXXXXXXXXXXXXXXXXXXSIAKAFPRIKCTVYDLPHVIADAPRTSFVDF 362
SALV+EC F +IA AFP IKCTVYDLPHVIAD+P S V
Sbjct: 172 SALVKECGNIFNGITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHC 231
Query: 363 VEGDMFKWVPKADAIMLKSILHDWGDTECIEILKKCREAVPREGGKVIILDIVLNAESEH 542
V GDMFK++PKADAIM+K ILHDW D ECIEILK+C+EAVP +GGKVII+DIVLN +SEH
Sbjct: 232 VAGDMFKFIPKADAIMMKCILHDWDDKECIEILKRCKEAVPVKGGKVIIVDIVLNVQSEH 291
Query: 543 PYAKTRLTLDLHMML-ISGKERTEEDWKKLIHDAGYRGYK 659
PY K RLTLDL MML GKERTEE+WKKLIHDAGY+G+K
Sbjct: 292 PYTKMRLTLDLDMMLNTGGKERTEEEWKKLIHDAGYKGHK 331
>gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictrum flavum subsp.
glaucum]
Length = 350
Score = 293 bits (751), Expect = 5e-77
Identities = 141/221 (63%), Positives = 170/221 (76%), Gaps = 2/221 (0%)
Frame = +3
Query: 3 ITDKDYMAPWHYLKDELSTENDV-TAFEKALGMSPWGYLAANPEKSQFFNEAMANDCSLI 179
ITDKD+MAPWHYLK+ + + TAFEKALG + W Y+A +PEK+Q FNE MAND LI
Sbjct: 114 ITDKDFMAPWHYLKEGILNDGSTSTAFEKALGTNIWDYMAEHPEKNQLFNEGMANDTRLI 173
Query: 180 MSALVRECSGSFXXXXXXXXXXXXXXXXXXSIAKAFPRIKCTVYDLPHVIADAPRTSFVD 359
MSALV+ECS F +IAKAFP IKCTVYDLPHVIAD+P + ++
Sbjct: 174 MSALVKECSSMFDGITTIVDVGGGTGTAVRNIAKAFPHIKCTVYDLPHVIADSPGYTEIN 233
Query: 360 FVEGDMFKWVPKADAIMLKSILHDWGDTECIEILKKCREAVPREGGKVIILDIVLNAESE 539
++GDMFK++P ADAIM+K ILHDW D ECIEILK+C++AVPR+GGKVII+DI+L+ +SE
Sbjct: 234 SIQGDMFKYIPNADAIMMKCILHDWDDKECIEILKRCKDAVPRDGGKVIIIDIILDVKSE 293
Query: 540 HPYAKTRLTLDLHMML-ISGKERTEEDWKKLIHDAGYRGYK 659
HPY K RLTLDL MML GKERTEE+WKKLIHDAGY+GYK
Sbjct: 294 HPYTKMRLTLDLDMMLNTGGKERTEEEWKKLIHDAGYKGYK 334
>gb|ACO90250.1| norcoclaurine 6-O-methyltransferase-like protein [Thalictrum
flavum]
Length = 346
Score = 277 bits (709), Expect = 3e-72
Identities = 135/222 (60%), Positives = 169/222 (76%), Gaps = 3/222 (1%)
Frame = +3
Query: 3 ITDKDYMAPWHYLKDELSTE-NDVTAFEKALGMSPWGYLAANPEKSQFFNEAMANDCSLI 179
+ D D+MAP+HY+KDE+ + + +TAFEKA G + W YLA +PEK+Q FNE MAN+ L
Sbjct: 109 MVDTDFMAPYHYIKDEILNDGSSITAFEKASGTNIWEYLAEHPEKNQLFNEGMANESRLT 168
Query: 180 MSALVRECSGSFXXXXXXXXXXXXXXXXXXSIAKAFPRIKCTVYDLPHVIADAPRTSFVD 359
MS L+++CS +F IAKAFP IK TVYDLPHVIADAP + ++
Sbjct: 169 MSGLMKDCSSTFEGLTTLVDVGGGTGTTVKYIAKAFPNIKYTVYDLPHVIADAPGDTEIN 228
Query: 360 FVEGDMFKWVPKADAIMLKSILHDWGDTECIEILKKCREAVPREGGKVIILDIVLNAESE 539
FVEGDMFK++P ADAI++K +LHDW D ECIEILK+C++AVPR+GGKVII+D+VLN +SE
Sbjct: 229 FVEGDMFKYIPNADAILMKGVLHDWDDNECIEILKRCKDAVPRDGGKVIIIDVVLNVKSE 288
Query: 540 HPYAKTRLTLDLHMMLIS-GKERTEEDWKKLIH-DAGYRGYK 659
HPY K RLTLDL MML + GKERTEEDWKKLI+ DAGY+GYK
Sbjct: 289 HPYTKMRLTLDLDMMLNNKGKERTEEDWKKLIYDDAGYKGYK 330
>gb|AKO60153.1| S-adenosyl-L-methionine:O-methyltransferase [Papaver somniferum]
Length = 348
Score = 261 bits (666), Expect = 3e-67
Identities = 130/220 (59%), Positives = 157/220 (71%), Gaps = 1/220 (0%)
Frame = +3
Query: 3 ITDKDYMAPWHYLKDELSTENDVTAFEKALGMSPWGYLAANPEKSQFFNEAMANDCSLIM 182
I DKD++APWH+LKD LS D AFEKALG S W Y++ NPEK+Q FNEAMA D L+
Sbjct: 115 INDKDFLAPWHHLKDGLS--GDCDAFEKALGKSIWVYMSENPEKNQLFNEAMACDTRLVT 172
Query: 183 SALVRECSGSFXXXXXXXXXXXXXXXXXXSIAKAFPRIKCTVYDLPHVIADAPRTSFVDF 362
SALV +C F +I+KAFP IKC++YDLPHVIAD+P V
Sbjct: 173 SALVNDCQSVFKGINTLVDVGGGTGTAVKAISKAFPHIKCSIYDLPHVIADSPEIPNVVK 232
Query: 363 VEGDMFKWVPKADAIMLKSILHDWGDTECIEILKKCREAVPREGGKVIILDIVLNAESEH 542
+EGDMFK +P ADAI++K ILHDW D ECI+ILKKC+EAVP+EGGKVII+D+VLN + H
Sbjct: 233 IEGDMFKAIPSADAILMKCILHDWNDDECIQILKKCKEAVPQEGGKVIIVDVVLNMDLTH 292
Query: 543 PYAKTRLTLDLHMML-ISGKERTEEDWKKLIHDAGYRGYK 659
PY+K RLTLDL MML GKERT E+WKKLI AG+ +K
Sbjct: 293 PYSKIRLTLDLDMMLNTGGKERTVEEWKKLIDAAGFASFK 332
>dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica]
Length = 351
Score = 260 bits (665), Expect = 4e-67
Identities = 127/220 (57%), Positives = 160/220 (72%), Gaps = 1/220 (0%)
Frame = +3
Query: 3 ITDKDYMAPWHYLKDELSTENDVTAFEKALGMSPWGYLAANPEKSQFFNEAMANDCSLIM 182
I DKD+M+PWH+LK+ L N+ AF+KALG S W Y++ANP+K++ FNE MA D L+
Sbjct: 118 INDKDFMSPWHHLKEGLV--NNSNAFDKALGKSIWDYMSANPDKNKVFNEGMACDSRLVS 175
Query: 183 SALVRECSGSFXXXXXXXXXXXXXXXXXXSIAKAFPRIKCTVYDLPHVIADAPRTSFVDF 362
SALV EC F +++KAFP IKC++YDLPHVI+D+P +
Sbjct: 176 SALVNECGNIFQGIQTLVDVGGGTGTAVKAVSKAFPHIKCSIYDLPHVISDSPDIPNIVK 235
Query: 363 VEGDMFKWVPKADAIMLKSILHDWGDTECIEILKKCREAVPREGGKVIILDIVLNAESEH 542
+EG+MFK +P ADAI++K ILHDW D ECIEILKKC+EAVP+EGGKVII+DIVL+ ES+H
Sbjct: 236 IEGEMFKSIPSADAILMKCILHDWNDDECIEILKKCKEAVPKEGGKVIIVDIVLDMESKH 295
Query: 543 PYAKTRLTLDLHMML-ISGKERTEEDWKKLIHDAGYRGYK 659
PY+K RLTLDL MML GKERTEE+WKKLI AG+ YK
Sbjct: 296 PYSKVRLTLDLDMMLNTEGKERTEEEWKKLIDGAGFASYK 335
>ref|XP_010244054.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Nelumbo
nucifera]
Length = 346
Score = 259 bits (661), Expect = 1e-66
Identities = 129/220 (58%), Positives = 161/220 (73%), Gaps = 1/220 (0%)
Frame = +3
Query: 3 ITDKDYMAPWHYLKDELSTENDVTAFEKALGMSPWGYLAANPEKSQFFNEAMANDCSLIM 182
+TDKD+MAPWH LKD L E TAFEKALG S W Y+A +PEK++ FNE MA D L++
Sbjct: 113 VTDKDFMAPWHCLKDSLCGEG--TAFEKALGRSIWTYMADHPEKNKLFNEGMACDTKLLI 170
Query: 183 SALVRECSGSFXXXXXXXXXXXXXXXXXXSIAKAFPRIKCTVYDLPHVIADAPRTSFVDF 362
SALV++C F +IAKAFP +KCT+YDLPHVIAD+P VD
Sbjct: 171 SALVQDCKDLFQGIMSLVDVGGGTGTAMRAIAKAFPHLKCTIYDLPHVIADSPDYPEVDR 230
Query: 363 VEGDMFKWVPKADAIMLKSILHDWGDTECIEILKKCREAVPREGGKVIILDIVLNAESEH 542
+ GDMFK +P ADAI+LK ILHDW D ECIEILK+C+E+VPREGGKVII+DIV++ ES+H
Sbjct: 231 IAGDMFKHIPSADAILLKCILHDWDDGECIEILKRCKESVPREGGKVIIVDIVVDLESKH 290
Query: 543 PYAKTRLTLDLHMMLIS-GKERTEEDWKKLIHDAGYRGYK 659
P KTRL+LDL MM+ + GKERTE +WKKL++ AG+ +K
Sbjct: 291 PLTKTRLSLDLDMMVTTGGKERTEAEWKKLLNAAGFPVFK 330
>gb|AJD20222.1| norcoclaurine 6-O-methyltransferase [Sinopodophyllum hexandrum]
Length = 350
Score = 253 bits (647), Expect = 5e-65
Identities = 128/219 (58%), Positives = 152/219 (69%), Gaps = 1/219 (0%)
Frame = +3
Query: 3 ITDKDYMAPWHYLKDELSTENDVTAFEKALGMSPWGYLAANPEKSQFFNEAMANDCSLIM 182
ITDK +M PW+Y+KDEL+ TAFE ALG W Y+ NPEK++ FN AMA D S+IM
Sbjct: 116 ITDKVFMEPWYYIKDELAPGTG-TAFELALGKDIWEYMGENPEKNKLFNAAMACDTSMIM 174
Query: 183 SALVRECSGSFXXXXXXXXXXXXXXXXXXSIAKAFPRIKCTVYDLPHVIADAPRTSFVDF 362
SAL+ EC F +IA+AFP IKCTVYDLPHVIAD+P ++
Sbjct: 175 SALISECKDKFNGIRTLVDVGGGTGTAARNIARAFPNIKCTVYDLPHVIADSPVYPEINR 234
Query: 363 VEGDMFKWVPKADAIMLKSILHDWGDTECIEILKKCREAVPREGGKVIILDIVLNAESEH 542
V GDMFK +P ADAI++K ILHDW D ECIEILK+C+EAVP +GGKVII+D+VL+ ESEH
Sbjct: 235 VSGDMFKCIPNADAILMKCILHDWEDKECIEILKRCKEAVPVDGGKVIIIDVVLDGESEH 294
Query: 543 PYAKTRLTLDLHMML-ISGKERTEEDWKKLIHDAGYRGY 656
PY K RL DL MML GKERT+E WKKL AGYR Y
Sbjct: 295 PYTKVRLNSDLDMMLNTEGKERTKEGWKKLFKAAGYRDY 333
>ref|XP_010247901.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Nelumbo
nucifera]
Length = 346
Score = 251 bits (642), Expect = 2e-64
Identities = 127/220 (57%), Positives = 156/220 (70%), Gaps = 1/220 (0%)
Frame = +3
Query: 3 ITDKDYMAPWHYLKDELSTENDVTAFEKALGMSPWGYLAANPEKSQFFNEAMANDCSLIM 182
ITD+D+ APWH LKD L+ E TAFEKALG S W Y+A +PEK++ FNE MA D S I
Sbjct: 113 ITDEDFFAPWHCLKDVLAGEG--TAFEKALGKSIWAYVADHPEKNKLFNEVMACDTSFIT 170
Query: 183 SALVRECSGSFXXXXXXXXXXXXXXXXXXSIAKAFPRIKCTVYDLPHVIADAPRTSFVDF 362
S L+++C F IAKAFP +KCT+YDLPHVIAD+P VD
Sbjct: 171 SVLIQDCKDVFQGIKSVVDVGGGTGTAMRDIAKAFPHLKCTIYDLPHVIADSPDYPEVDR 230
Query: 363 VEGDMFKWVPKADAIMLKSILHDWGDTECIEILKKCREAVPREGGKVIILDIVLNAESEH 542
+ GDMFK +P ADAI+LK ILHDW D ECIEILK+C+E+VPREGGKVII+DIVL+ ES+
Sbjct: 231 IAGDMFKHIPSADAILLKWILHDWDDGECIEILKRCKESVPREGGKVIIVDIVLDPESKD 290
Query: 543 PYAKTRLTLDLHMMLIS-GKERTEEDWKKLIHDAGYRGYK 659
P K RL LDL MM+ + GKER+E +WKKL++ AG+ GYK
Sbjct: 291 PLTKARLRLDLDMMVYTGGKERSEAEWKKLLNAAGFPGYK 330
>gb|AKH61490.1| 6-O-methyltransferase [Expression vector pWCD2353]
Length = 348
Score = 236 bits (603), Expect = 7e-60
Identities = 121/221 (54%), Positives = 151/221 (68%), Gaps = 2/221 (0%)
Frame = +3
Query: 3 ITDKDYMAPWHYLKDELSTENDVTAFEKALGMSPWGYLAANPEKSQFFNEAMANDCSLIM 182
I DKD++APWH+L D L+ D AFEKALG S W Y++ NPEK+Q FN AMA D L+
Sbjct: 113 INDKDFLAPWHHLGDGLTGNCD--AFEKALGKSIWVYMSVNPEKNQLFNAAMACDTRLVT 170
Query: 183 SALVRECSGSFXXXXXXXXXXXXXXXXXX-SIAKAFPRIKCTVYDLPHVIADAPRTSFVD 359
SAL EC F +I+KAFP IKCT+YDLPHVIAD+P +
Sbjct: 171 SALANECKSIFSDGISTLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNIT 230
Query: 360 FVEGDMFKWVPKADAIMLKSILHDWGDTECIEILKKCREAVPREGGKVIILDIVLNAESE 539
+ GDMFK +P ADAI +K ILHDW D ECI+ILK+C+EA+P+ GGKVII+D+V++ +S
Sbjct: 231 KISGDMFKSIPSADAIFMKCILHDWNDDECIQILKRCKEALPK-GGKVIIVDVVIDMDST 289
Query: 540 HPYAKTRLTLDLHMML-ISGKERTEEDWKKLIHDAGYRGYK 659
HPYAK RLTLDL MML GKERT+E+WK L AG+ +K
Sbjct: 290 HPYAKIRLTLDLDMMLNTGGKERTKEEWKTLFDAAGFASHK 330
>sp|Q6WUC1.1|6OMT_PAPSO RecName: Full=(RS)-norcoclaurine 6-O-methyltransferase;
Short=Ps6OMT gi|33286374|gb|AAQ01669.1|
(R,S)-norcoclaurine 6-O-methyltransferase [Papaver
somniferum] gi|571330878|gb|AHF27395.1|
(R,S)-norcoclaurine 6-O-methyltransferase [synthetic
construct]
Length = 346
Score = 236 bits (603), Expect = 7e-60
Identities = 121/221 (54%), Positives = 151/221 (68%), Gaps = 2/221 (0%)
Frame = +3
Query: 3 ITDKDYMAPWHYLKDELSTENDVTAFEKALGMSPWGYLAANPEKSQFFNEAMANDCSLIM 182
I DKD++APWH+L D L+ D AFEKALG S W Y++ NPEK+Q FN AMA D L+
Sbjct: 113 INDKDFLAPWHHLGDGLTGNCD--AFEKALGKSIWVYMSVNPEKNQLFNAAMACDTRLVT 170
Query: 183 SALVRECSGSFXXXXXXXXXXXXXXXXXX-SIAKAFPRIKCTVYDLPHVIADAPRTSFVD 359
SAL EC F +I+KAFP IKCT+YDLPHVIAD+P +
Sbjct: 171 SALANECKSIFSDGISTLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNIT 230
Query: 360 FVEGDMFKWVPKADAIMLKSILHDWGDTECIEILKKCREAVPREGGKVIILDIVLNAESE 539
+ GDMFK +P ADAI +K ILHDW D ECI+ILK+C+EA+P+ GGKVII+D+V++ +S
Sbjct: 231 KISGDMFKSIPSADAIFMKCILHDWNDDECIQILKRCKEALPK-GGKVIIVDVVIDMDST 289
Query: 540 HPYAKTRLTLDLHMML-ISGKERTEEDWKKLIHDAGYRGYK 659
HPYAK RLTLDL MML GKERT+E+WK L AG+ +K
Sbjct: 290 HPYAKIRLTLDLDMMLNTGGKERTKEEWKTLFDAAGFASHK 330
>gb|AKN62837.1| norcoclaurine 6-O-methyltransferase, partial [Papaver orientale]
Length = 249
Score = 234 bits (596), Expect = 4e-59
Identities = 120/221 (54%), Positives = 151/221 (68%), Gaps = 2/221 (0%)
Frame = +3
Query: 3 ITDKDYMAPWHYLKDELSTENDVTAFEKALGMSPWGYLAANPEKSQFFNEAMANDCSLIM 182
I DKD++APWH+L D L+ D AFEKALG S W Y++ NPEK+Q FN AMA D L+
Sbjct: 26 INDKDFLAPWHHLGDGLTGNCD--AFEKALGKSIWVYMSENPEKNQLFNAAMACDTRLVT 83
Query: 183 SALVRECSGSFXXXXXXXXXXXXXXXXXX-SIAKAFPRIKCTVYDLPHVIADAPRTSFVD 359
SAL EC F +I+KAFP IKCT+YDLPHVIAD+P +
Sbjct: 84 SALANECKSIFSDGIRTLVDVGGGTGTAVKTISKAFPDIKCTIYDLPHVIADSPEIPNIT 143
Query: 360 FVEGDMFKWVPKADAIMLKSILHDWGDTECIEILKKCREAVPREGGKVIILDIVLNAESE 539
+ GDMFK +P ADAI +K ILHDW D ECI+ILK+C+EA+P++ GKVII+D+V++ +S
Sbjct: 144 KISGDMFKSIPSADAIFMKCILHDWNDEECIQILKRCKEALPKD-GKVIIVDVVIDMDST 202
Query: 540 HPYAKTRLTLDLHMML-ISGKERTEEDWKKLIHDAGYRGYK 659
HPYAK RLTLDL MML GKERT+E+WK L AG+ +K
Sbjct: 203 HPYAKIRLTLDLDMMLNTGGKERTKEEWKILFDAAGFASHK 243
>gb|AKN62836.1| norcoclaurine 6-O-methyltransferase, partial [Papaver
pseudo-orientale]
Length = 343
Score = 234 bits (596), Expect = 4e-59
Identities = 120/221 (54%), Positives = 151/221 (68%), Gaps = 2/221 (0%)
Frame = +3
Query: 3 ITDKDYMAPWHYLKDELSTENDVTAFEKALGMSPWGYLAANPEKSQFFNEAMANDCSLIM 182
I DKD++APWH+L D L+ D AFEKALG S W Y++ NPEK+Q FN AMA D L+
Sbjct: 111 INDKDFLAPWHHLGDGLTGNCD--AFEKALGKSIWVYMSENPEKNQLFNAAMACDTRLVT 168
Query: 183 SALVRECSGSFXXXXXXXXXXXXXXXXXX-SIAKAFPRIKCTVYDLPHVIADAPRTSFVD 359
SAL EC F +I+KAFP IKCT+YDLPHVIAD+P +
Sbjct: 169 SALANECKSIFSDGIRTLVDVGGGTGTAVKTISKAFPDIKCTIYDLPHVIADSPEIPNIT 228
Query: 360 FVEGDMFKWVPKADAIMLKSILHDWGDTECIEILKKCREAVPREGGKVIILDIVLNAESE 539
+ GDMFK +P ADAI +K ILHDW D ECI+ILK+C+EA+P++ GKVII+D+V++ +S
Sbjct: 229 KISGDMFKSIPSADAIFMKCILHDWSDEECIQILKRCKEALPKD-GKVIIVDVVIDMDST 287
Query: 540 HPYAKTRLTLDLHMML-ISGKERTEEDWKKLIHDAGYRGYK 659
HPYAK RLTLDL MML GKERT+E+WK L AG+ +K
Sbjct: 288 HPYAKIRLTLDLDMMLNTGGKERTKEEWKILFDAAGFASHK 328
>gb|ACO90232.1| putative norcoclaurine 6-O-methyltransferase [Papaver bracteatum]
Length = 346
Score = 234 bits (596), Expect = 4e-59
Identities = 120/221 (54%), Positives = 151/221 (68%), Gaps = 2/221 (0%)
Frame = +3
Query: 3 ITDKDYMAPWHYLKDELSTENDVTAFEKALGMSPWGYLAANPEKSQFFNEAMANDCSLIM 182
I DKD++APWH+L D L+ D AFEKALG S W Y++ NPEK+Q FN AMA D L+
Sbjct: 113 INDKDFLAPWHHLGDGLTGNCD--AFEKALGKSIWVYMSENPEKNQLFNAAMACDTRLVT 170
Query: 183 SALVRECSGSFXXXXXXXXXXXXXXXXXX-SIAKAFPRIKCTVYDLPHVIADAPRTSFVD 359
SAL EC F +I+KAFP IKCT+YDLPHVIAD+P +
Sbjct: 171 SALANECKSIFSDGIKTLVDVGGGTGTAVKTISKAFPDIKCTIYDLPHVIADSPEIPNIT 230
Query: 360 FVEGDMFKWVPKADAIMLKSILHDWGDTECIEILKKCREAVPREGGKVIILDIVLNAESE 539
+ GDMFK +P ADAI +K ILHDW D ECI+ILK+C+EA+P++ GKVII+D+V++ +S
Sbjct: 231 KISGDMFKSIPSADAIFMKCILHDWNDEECIQILKRCKEALPKD-GKVIIVDVVIDMDST 289
Query: 540 HPYAKTRLTLDLHMML-ISGKERTEEDWKKLIHDAGYRGYK 659
HPYAK RLTLDL MML GKERT+E+WK L AG+ +K
Sbjct: 290 HPYAKIRLTLDLDMMLNTGGKERTKEEWKILFDAAGFASHK 330
>gb|AAP45315.1| S-adenosyl-L-methionine:norcoclaurine 6-O-methyltransferase
[Papaver somniferum]
Length = 346
Score = 233 bits (594), Expect = 8e-59
Identities = 120/221 (54%), Positives = 150/221 (67%), Gaps = 2/221 (0%)
Frame = +3
Query: 3 ITDKDYMAPWHYLKDELSTENDVTAFEKALGMSPWGYLAANPEKSQFFNEAMANDCSLIM 182
I DKD++APWH+L D L+ D AFEKALG S W Y++ NPEK+Q FN AMA D L+
Sbjct: 113 INDKDFLAPWHHLGDGLTGNCD--AFEKALGKSIWVYMSENPEKNQLFNAAMACDTRLVT 170
Query: 183 SALVRECSGSFXXXXXXXXXXXXXXXXXX-SIAKAFPRIKCTVYDLPHVIADAPRTSFVD 359
SAL EC F +I+KAFP IKCT+YDLPHVIAD+P +
Sbjct: 171 SALANECKSIFSDGISTLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNIT 230
Query: 360 FVEGDMFKWVPKADAIMLKSILHDWGDTECIEILKKCREAVPREGGKVIILDIVLNAESE 539
+ GDMFK +P ADAI +K ILHDW D ECI+ILK+C+EA+P+ GKVII+D+V++ +S
Sbjct: 231 KISGDMFKSIPSADAIFMKCILHDWNDDECIQILKRCKEALPKV-GKVIIVDVVIDMDST 289
Query: 540 HPYAKTRLTLDLHMML-ISGKERTEEDWKKLIHDAGYRGYK 659
HPYAK RLTLDL MML GKERT+E+WK L AG+ +K
Sbjct: 290 HPYAKIRLTLDLDMMLNTGGKERTKEEWKTLFDAAGFASHK 330
>ref|XP_010271691.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Nelumbo
nucifera]
Length = 351
Score = 231 bits (589), Expect = 3e-58
Identities = 118/221 (53%), Positives = 149/221 (67%), Gaps = 2/221 (0%)
Frame = +3
Query: 3 ITDKDYMAPWHYLKDELSTENDVTAFEKALGMSPWGYLAANPEKSQFFNEAMANDCSLIM 182
ITDKD++APWH+LKD L+ + + T FEK LG S Y+A + EKS FNE+M +D L
Sbjct: 114 ITDKDFIAPWHHLKDSLAGDGEETTFEKVLGKSISTYMADHLEKSMLFNESMVHDTRLFT 173
Query: 183 SALVRECSGSFXXXXXXXXXXXXXXXXXXSIAKAFPRIKCTVYDLPHVIADAPRTSFVDF 362
S L+++ F +IAKAFP +KCT+Y LPHVIAD+P VD
Sbjct: 174 SVLIQDFKDVFQGIKSLVDVGGGSGTDMGAIAKAFPHLKCTIYGLPHVIADSPDYPEVDR 233
Query: 363 VEGDMFKWVPKADAIMLKSILHDWGDTECIEILKKCREAVPREGGKVIILDIVLNAESEH 542
+ GDMFK +P ADAI+LK ILH WGD +CIEILK+C+E+VPREGG VII D V++ ES+H
Sbjct: 234 ISGDMFKHIPSADAILLKCILHYWGDGQCIEILKRCKESVPREGGIVIIADAVVDLESKH 293
Query: 543 PY-AKTRLTLDLHMML-ISGKERTEEDWKKLIHDAGYRGYK 659
PY KT L+ DL MML GKERTE +WKKL + AG+ YK
Sbjct: 294 PYLTKTLLSTDLDMMLNTGGKERTEAEWKKLFNAAGFPAYK 334
>gb|AKO60152.1| S-adenosyl-L-methionine:O-methyltransferase [Papaver somniferum]
Length = 353
Score = 226 bits (575), Expect = 1e-56
Identities = 109/220 (49%), Positives = 146/220 (66%), Gaps = 1/220 (0%)
Frame = +3
Query: 3 ITDKDYMAPWHYLKDELSTENDVTAFEKALGMSPWGYLAANPEKSQFFNEAMANDCSLIM 182
+T KD+M PWH++K+ L ND TAFEK +GM+ W YL +PE+S FNE MA + L+
Sbjct: 121 MTQKDFMIPWHFMKEGLG--NDTTAFEKGMGMTIWQYLEGHPEQSNLFNEGMAGETRLLT 178
Query: 183 SALVRECSGSFXXXXXXXXXXXXXXXXXXSIAKAFPRIKCTVYDLPHVIADAPRTSFVDF 362
+L+ C +F I AFP+IKC+VYDLPHVIA +P ++
Sbjct: 179 KSLIDGCRDTFEGLTSLCDVGGGNGTTIKGIYDAFPQIKCSVYDLPHVIASSPEHPNIER 238
Query: 363 VEGDMFKWVPKADAIMLKSILHDWGDTECIEILKKCREAVPREGGKVIILDIVLNAESEH 542
+ GDMFK VP A AI+LK ILHDW D EC+ IL KCREAVP++ GKVII+D+ L ES+H
Sbjct: 239 IPGDMFKSVPSAQAILLKLILHDWTDEECVNILIKCREAVPKDTGKVIIVDVALEEESQH 298
Query: 543 PYAKTRLTLDLHMML-ISGKERTEEDWKKLIHDAGYRGYK 659
KTRL LD+ M++ G+ER+E+DW+KL+ AG+RG+K
Sbjct: 299 ELTKTRLILDIDMLVNTGGRERSEDDWEKLLKRAGFRGHK 338
>ref|XP_010247902.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Nelumbo
nucifera]
Length = 347
Score = 224 bits (570), Expect = 5e-56
Identities = 118/221 (53%), Positives = 151/221 (68%), Gaps = 2/221 (0%)
Frame = +3
Query: 3 ITDKDYMAPWHYLKDELSTENDVTAFEKALGMSPWGYLAANPEKSQFFNEAMANDCS-LI 179
+TDKD+M PW+ LKD L E TAFEKALG + +A +PEK + FNEAMA D + L+
Sbjct: 113 VTDKDFMVPWYRLKDSLVGEG--TAFEKALGKTICECMADHPEKKKPFNEAMACDTTRLL 170
Query: 180 MSALVRECSGSFXXXXXXXXXXXXXXXXXXSIAKAFPRIKCTVYDLPHVIADAPRTSFVD 359
SAL+++C F IAK FP +KCT+YDLPHVIAD+P VD
Sbjct: 171 TSALIQDCKDLFQGIMSLVDVGGGTGTAMRDIAKTFPHLKCTIYDLPHVIADSPDYPEVD 230
Query: 360 FVEGDMFKWVPKADAIMLKSILHDWGDTECIEILKKCREAVPREGGKVIILDIVLNAESE 539
+ G+MFK +P AD I+LK ILHD GD +CIEIL++C+E+VPREGGKVII+DIVL+ ES
Sbjct: 231 RIAGNMFKHIPSADGILLKCILHDLGDRQCIEILQRCKESVPREGGKVIIVDIVLDPEST 290
Query: 540 HPYAKTRLTLDLHMMLIS-GKERTEEDWKKLIHDAGYRGYK 659
P K RL LDL MM+ + GKER+E +WKKL++ AG+ YK
Sbjct: 291 DPLTKARLRLDLDMMVYTGGKERSEAEWKKLLNAAGFPRYK 331
>gb|AKH61488.1| 4' O-methyltransferase [Expression vector pWCD2353]
Length = 359
Score = 218 bits (554), Expect = 3e-54
Identities = 104/220 (47%), Positives = 141/220 (64%), Gaps = 1/220 (0%)
Frame = +3
Query: 3 ITDKDYMAPWHYLKDELSTENDVTAFEKALGMSPWGYLAANPEKSQFFNEAMANDCSLIM 182
+T KD+M WH++K+ L TAFEK +GM W YL NP++SQ FNE MA + L+
Sbjct: 124 MTQKDFMVSWHFMKEGLGN-GSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLT 182
Query: 183 SALVRECSGSFXXXXXXXXXXXXXXXXXXSIAKAFPRIKCTVYDLPHVIADAPRTSFVDF 362
L+ +C +F +I +AFP IKCT+YDLPHV+A++ ++
Sbjct: 183 KTLIEDCRDTFQGLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPNIEK 242
Query: 363 VEGDMFKWVPKADAIMLKSILHDWGDTECIEILKKCREAVPREGGKVIILDIVLNAESEH 542
V GDMFK VP A AI+LK ILHDW D EC+ ILKKC+EA+P+E GKVII+D+ L ES H
Sbjct: 243 VPGDMFKSVPSAQAILLKLILHDWTDEECVNILKKCKEAIPKETGKVIIVDVALEEESNH 302
Query: 543 PYAKTRLTLDLHMML-ISGKERTEEDWKKLIHDAGYRGYK 659
KTRL LD+ M++ G+ERT +DW+ L+ AG+R +K
Sbjct: 303 ELTKTRLILDIDMLVNTGGRERTADDWENLLKRAGFRSHK 342
>gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase
[Thalictrum flavum subsp. glaucum]
Length = 348
Score = 218 bits (554), Expect = 3e-54
Identities = 107/220 (48%), Positives = 150/220 (68%), Gaps = 1/220 (0%)
Frame = +3
Query: 3 ITDKDYMAPWHYLKDELSTENDVTAFEKALGMSPWGYLAANPEKSQFFNEAMANDCSLIM 182
+T KD+M PWH +KD L T+N VTAFEKA+GM+ W YL +P++SQ FNE MA + L+
Sbjct: 115 MTQKDFMTPWHSMKDGL-TDNGVTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLT 173
Query: 183 SALVRECSGSFXXXXXXXXXXXXXXXXXXSIAKAFPRIKCTVYDLPHVIADAPRTSFVDF 362
S+L+ F +I+ AFP IKCT++DLPHVIA++ ++
Sbjct: 174 SSLISGSKDMFQSIDSLVDVGGGNGTTIKAISVAFPHIKCTLFDLPHVIANSYDHPNIER 233
Query: 363 VEGDMFKWVPKADAIMLKSILHDWGDTECIEILKKCREAVPREGGKVIILDIVLNAESEH 542
+ GDMFK +P A AI+LK ILHDW D + I+ILK+CR+AVP++GGKVII+D+ L+ +S+H
Sbjct: 234 IGGDMFKGMPSAQAILLKLILHDWNDEDSIKILKQCRKAVPKDGGKVIIVDVALDEDSDH 293
Query: 543 PYAKTRLTLDLHMML-ISGKERTEEDWKKLIHDAGYRGYK 659
+ TRL LD+ M++ GKERT+EDW+KL+ AG+ G K
Sbjct: 294 ELSSTRLILDIDMLVNTGGKERTKEDWEKLVKCAGFSGCK 333
>sp|Q7XB10.1|4OMT2_PAPSO RecName: Full=3'-hydroxy-N-methyl-(S)-coclaurine
4'-O-methyltransferase 2; Short=4'-OMT2; Short=Ps4'OMT2;
AltName:
Full=S-adenosyl-L-methionine:3'-hydroxy-N-
methylcoclaurine 4'-O-methyltransferase
gi|33413894|gb|AAP45314.1|
S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
4'-O-methyltransferase 2 [Papaver somniferum]
gi|571330882|gb|AHF27397.1|
S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
4'- O-methyltransferase 2 [synthetic construct]
Length = 357
Score = 218 bits (554), Expect = 3e-54
Identities = 104/220 (47%), Positives = 141/220 (64%), Gaps = 1/220 (0%)
Frame = +3
Query: 3 ITDKDYMAPWHYLKDELSTENDVTAFEKALGMSPWGYLAANPEKSQFFNEAMANDCSLIM 182
+T KD+M WH++K+ L TAFEK +GM W YL NP++SQ FNE MA + L+
Sbjct: 124 MTQKDFMVSWHFMKEGLGN-GSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLT 182
Query: 183 SALVRECSGSFXXXXXXXXXXXXXXXXXXSIAKAFPRIKCTVYDLPHVIADAPRTSFVDF 362
L+ +C +F +I +AFP IKCT+YDLPHV+A++ ++
Sbjct: 183 KTLIEDCRDTFQGLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPNIEK 242
Query: 363 VEGDMFKWVPKADAIMLKSILHDWGDTECIEILKKCREAVPREGGKVIILDIVLNAESEH 542
V GDMFK VP A AI+LK ILHDW D EC+ ILKKC+EA+P+E GKVII+D+ L ES H
Sbjct: 243 VPGDMFKSVPSAQAILLKLILHDWTDEECVNILKKCKEAIPKETGKVIIVDVALEEESNH 302
Query: 543 PYAKTRLTLDLHMML-ISGKERTEEDWKKLIHDAGYRGYK 659
KTRL LD+ M++ G+ERT +DW+ L+ AG+R +K
Sbjct: 303 ELTKTRLILDIDMLVNTGGRERTADDWENLLKRAGFRSHK 342