BLASTX nr result
ID: Aconitum23_contig00019136
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Aconitum23_contig00019136
(1612 letters)
Database: ./nr
77,306,371 sequences; 28,104,191,420 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. diss... 445 0.0
gb|AJD20229.1| corytuberine synthase [Sinopodophyllum hexandrum] 420 0.0
sp|P47195.1|C80A1_BERST RecName: Full=Berbamunine synthase; AltN... 334 e-133
gb|AGC29949.1| CYP80G3 [Sinopodophyllum hexandrum] 420 e-132
ref|XP_010253990.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 310 e-128
ref|XP_010253989.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 308 e-125
sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcocla... 288 e-125
gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chi... 286 e-124
sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-... 287 e-124
gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase ... 286 e-124
sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-... 286 e-123
sp|Q9SP06.1|C80B3_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-... 282 e-120
gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [P... 282 e-120
gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [... 281 e-120
emb|CBI31830.3| unnamed protein product [Vitis vinifera] 300 e-118
ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozym... 296 e-118
ref|XP_010662515.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 300 e-118
ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 293 e-116
emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera] 293 e-116
gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum... 273 e-116
>dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. dissecta]
Length = 486
Score = 445 bits (1144), Expect(2) = 0.0
Identities = 212/266 (79%), Positives = 241/266 (90%)
Frame = -2
Query: 834 FFPILEGLDLQGLKKKCHDRFVRVMNLWKGTVEERKQKRNEESKDMLDVLLANDFNDAQI 655
F+P LE LDLQGLKKKC +RF+RVM +W+GTV+ERK RNEESKDMLDVLLANDFNDAQI
Sbjct: 221 FYPSLEELDLQGLKKKCDERFIRVMKMWEGTVKERKANRNEESKDMLDVLLANDFNDAQI 280
Query: 654 NALFLETFGPGSETSSATIEWVMAELIKNPDKMAKVREELDTVVGNSTVKESHLPQLPYL 475
NALFLETFGPGSETSSATIEWV+AELIK+P +MAKVR+EL+ VVG ST+KES LPQLPYL
Sbjct: 281 NALFLETFGPGSETSSATIEWVIAELIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYL 340
Query: 474 HACIKEAMRLHPAAPFLLPRRAIESCEIMGYTIPKDCQLLVNAYAIGRDPKAWKDPTTFR 295
ACIKEAMRLHPAAPFLLPRRA E+CE+MGYTIPK+ Q+LVNAYAIGRDPK+WKDP+TF
Sbjct: 341 QACIKEAMRLHPAAPFLLPRRAAETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFW 400
Query: 294 PERFLESDVDYHGSHYQFIPFGAGRRICIGMPLATRTIPLIVGSLVHTYDWSLPNGRGAD 115
PERFLESDVD+HG+HYQFIPFG+GRR C+GMPLATRTIPLIVGSLVH YD+ LP G +
Sbjct: 401 PERFLESDVDFHGAHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPE 460
Query: 114 ALEMKENLTLSLAIDPSLCIVPNSRA 37
L+M E L+L+LAIDPSLC+VP +RA
Sbjct: 461 DLKMNEMLSLTLAIDPSLCVVPKARA 486
Score = 307 bits (786), Expect(2) = 0.0
Identities = 153/201 (76%), Positives = 168/201 (83%), Gaps = 2/201 (0%)
Frame = -3
Query: 1442 MDLQVALFCTIPXXXXXXLASKLKRPNLPPGPHAWPLIGSLPILFSNPDVPLHITLTNMA 1263
MDLQ+ALF IP L K K NLPPGPH WPLIG+LPILF+N +VPLHITL NMA
Sbjct: 1 MDLQIALFSLIPVILVFILLLKPKYKNLPPGPHPWPLIGNLPILFTNTEVPLHITLANMA 60
Query: 1262 RTHGPLMTLWLGQQSTVVASSPEAATEILKIHDRACSGRYIRMSFRLKHHIKYSLVWSDC 1083
RTHGP+M LWLG Q TV+AS+ EAA EILK HDR S R+IRMSFRLKHHIKYSLVWSDC
Sbjct: 61 RTHGPIMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHIRMSFRLKHHIKYSLVWSDC 120
Query: 1082 TDYWKLLRKIVRTEIFSPKMLQAQSHVREERVGELMDYLRSKQGQTVKIGKFVFGTLLNI 903
TDYWKLLRKIVRTEIFSPKMLQAQSHVRE++V EL+D+LRSK+GQ VKI +FVFGTLLNI
Sbjct: 121 TDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVKISQFVFGTLLNI 180
Query: 902 LGNAVFSKNVFVYSD--DDGG 846
LGN VFSK+VFVY D D GG
Sbjct: 181 LGNVVFSKDVFVYGDETDKGG 201
>gb|AJD20229.1| corytuberine synthase [Sinopodophyllum hexandrum]
Length = 483
Score = 420 bits (1080), Expect(2) = 0.0
Identities = 200/265 (75%), Positives = 227/265 (85%)
Frame = -2
Query: 834 FFPILEGLDLQGLKKKCHDRFVRVMNLWKGTVEERKQKRNEESKDMLDVLLANDFNDAQI 655
F+PI E DLQGLKK+C+ RF VM +WKGTVEERK KRNEE KDMLDVLL NDFNDAQI
Sbjct: 218 FWPIFEEFDLQGLKKRCNQRFSEVMKMWKGTVEERKVKRNEEPKDMLDVLLLNDFNDAQI 277
Query: 654 NALFLETFGPGSETSSATIEWVMAELIKNPDKMAKVREELDTVVGNSTVKESHLPQLPYL 475
NA+FLETFGPGSETSSAT EW +AELIKNP +AKVR+ELD VVG STVKES LP LPYL
Sbjct: 278 NAVFLETFGPGSETSSATSEWALAELIKNPHILAKVRQELDQVVGKSTVKESDLPHLPYL 337
Query: 474 HACIKEAMRLHPAAPFLLPRRAIESCEIMGYTIPKDCQLLVNAYAIGRDPKAWKDPTTFR 295
ACIKE MRLHPAAPFLLP RA+E+C +MGYTIPKD Q+LVN YAIGRDPK WK+PTTF+
Sbjct: 338 QACIKETMRLHPAAPFLLPHRAVETCPVMGYTIPKDTQVLVNIYAIGRDPKTWKEPTTFK 397
Query: 294 PERFLESDVDYHGSHYQFIPFGAGRRICIGMPLATRTIPLIVGSLVHTYDWSLPNGRGAD 115
PERFLESDVDY+G H++FIPFGAGRR C+GMPLATRTIPLIV +LV T DWSLP+G+
Sbjct: 398 PERFLESDVDYYGKHFEFIPFGAGRRQCVGMPLATRTIPLIVSNLVQTLDWSLPDGKLPQ 457
Query: 114 ALEMKENLTLSLAIDPSLCIVPNSR 40
L M E+L+LSLA+DP+L ++P SR
Sbjct: 458 DLVMNESLSLSLALDPTLTVIPKSR 482
Score = 262 bits (670), Expect(2) = 0.0
Identities = 130/200 (65%), Positives = 155/200 (77%)
Frame = -3
Query: 1442 MDLQVALFCTIPXXXXXXLASKLKRPNLPPGPHAWPLIGSLPILFSNPDVPLHITLTNMA 1263
M+L AL IP L K + NLPPGP WP+IG++P LFS+ P+H+ LTNMA
Sbjct: 1 MELTTALLYLIPTILLYILF-KPRSKNLPPGPKPWPIIGNIPALFSSA-YPMHVALTNMA 58
Query: 1262 RTHGPLMTLWLGQQSTVVASSPEAATEILKIHDRACSGRYIRMSFRLKHHIKYSLVWSDC 1083
RTHGP+M LWLG Q T VAS+ EAA EILK HDR+ SGR +RMSFRLKHHIKYSLVW+DC
Sbjct: 59 RTHGPIMILWLGTQPTAVASTTEAAMEILKTHDRSFSGRAVRMSFRLKHHIKYSLVWADC 118
Query: 1082 TDYWKLLRKIVRTEIFSPKMLQAQSHVREERVGELMDYLRSKQGQTVKIGKFVFGTLLNI 903
++YWKLLRKIVRTEIFSPKMLQ Q HVRE++V EL+++L+ K+GQ VKI + VFGTLLNI
Sbjct: 119 SEYWKLLRKIVRTEIFSPKMLQVQEHVREQKVAELVEFLKGKEGQVVKISQLVFGTLLNI 178
Query: 902 LGNAVFSKNVFVYSDDDGGK 843
LGN +FSK+VF Y DG K
Sbjct: 179 LGNVIFSKDVFRYVGADGDK 198
>sp|P47195.1|C80A1_BERST RecName: Full=Berbamunine synthase; AltName:
Full=(S)-N-methylcoclaurine oxidase [C-O
phenol-coupling]; AltName: Full=CYPLXXX; AltName:
Full=Cytochrome P450 80 gi|642386|gb|AAC48987.1|
cytochrome P-450 CYP80 [Berberis stolonifera]
Length = 487
Score = 334 bits (857), Expect(2) = e-133
Identities = 155/266 (58%), Positives = 204/266 (76%), Gaps = 1/266 (0%)
Frame = -2
Query: 834 FFPILEGLDLQGLKKKCHDRFVRVMNLWKGTVEERKQKRNEESKDMLDVLLANDFNDAQI 655
+FP+L DL GLK+K ++F ++ +W+G V R+ RN E KDMLDVL+ANDFN+ QI
Sbjct: 221 YFPVLARFDLHGLKRKMDEQFKLLIKIWEGEVLARRANRNPEPKDMLDVLIANDFNEHQI 280
Query: 654 NALFLETFGPGSETSSATIEWVMAELIKNPDKMAKVREELDTVVG-NSTVKESHLPQLPY 478
NA+F+ETFGPGS+T+S IEW +A+LIKNPDK+AK+REELD VVG +STVKESH +LPY
Sbjct: 281 NAMFMETFGPGSDTNSNIIEWALAQLIKNPDKLAKLREELDRVVGRSSTVKESHFSELPY 340
Query: 477 LHACIKEAMRLHPAAPFLLPRRAIESCEIMGYTIPKDCQLLVNAYAIGRDPKAWKDPTTF 298
L AC+KE MRL+P ++P R +E+C++MGYTIPK + VNA+AIGRDPK WKDP F
Sbjct: 341 LQACVKETMRLYPPISIMIPHRCMETCQVMGYTIPKGMDVHVNAHAIGRDPKDWKDPLKF 400
Query: 297 RPERFLESDVDYHGSHYQFIPFGAGRRICIGMPLATRTIPLIVGSLVHTYDWSLPNGRGA 118
+PERFL+SD++Y+G +QFIPFG+GRRIC G PLA R IPL++ SLVH + W LP+G
Sbjct: 401 QPERFLDSDIEYNGKQFQFIPFGSGRRICPGRPLAVRIIPLVLASLVHAFGWELPDGVPN 460
Query: 117 DALEMKENLTLSLAIDPSLCIVPNSR 40
+ L+M+E TLSL + L ++P R
Sbjct: 461 EKLDMEELFTLSLCMAKPLRVIPKVR 486
Score = 170 bits (430), Expect(2) = e-133
Identities = 87/182 (47%), Positives = 119/182 (65%), Gaps = 2/182 (1%)
Frame = -3
Query: 1379 KLKRPNLPPGPHAWPLIGSLPILFSNPDVPLHITLTNMARTHGPLMTLWLGQQSTVVASS 1200
K K NLPP P AWP++G LP L S P ++N+A+ +GPL+ L G S++ AS+
Sbjct: 22 KPKHTNLPPSPPAWPIVGHLPDLISKNSPPFLDYMSNIAQKYGPLIHLKFGLHSSIFAST 81
Query: 1199 PEAATEILKIHDRACSGRYIRMSFRLKHHIKYSLVWSDCTDYWKLLRKIVRTEIFSPKML 1020
EAA E+L+ +D+ SGR FR+K HI YS++WSD YWK RKI+ TEIFS KML
Sbjct: 82 KEAAMEVLQTNDKVLSGRQPLPCFRIKPHIDYSILWSDSNSYWKKGRKILHTEIFSQKML 141
Query: 1019 QAQSHVREERVGELMDYLRSKQGQTVKIGKFVFGTLLNILGNAVFSKNVFVYSD--DDGG 846
QAQ RE G L++++ +K G V++ ++FG LN+LG+ VFSK+VF YSD D+ G
Sbjct: 142 QAQEKNRERVAGNLVNFIMTKVGDVVELRSWLFGCALNVLGHVVFSKDVFEYSDQSDEVG 201
Query: 845 KD 840
D
Sbjct: 202 MD 203
>gb|AGC29949.1| CYP80G3 [Sinopodophyllum hexandrum]
Length = 346
Score = 420 bits (1080), Expect(2) = e-132
Identities = 200/265 (75%), Positives = 227/265 (85%)
Frame = -2
Query: 834 FFPILEGLDLQGLKKKCHDRFVRVMNLWKGTVEERKQKRNEESKDMLDVLLANDFNDAQI 655
F+PI E DLQGLKK+C+ RF VM +WKGTVEERK KRNEE KDMLDVLL NDFNDAQI
Sbjct: 81 FWPIFEEFDLQGLKKRCNQRFSEVMKMWKGTVEERKVKRNEEPKDMLDVLLLNDFNDAQI 140
Query: 654 NALFLETFGPGSETSSATIEWVMAELIKNPDKMAKVREELDTVVGNSTVKESHLPQLPYL 475
NA+FLETFGPGSETSSAT EW +AELIKNP +AKVR+ELD VVG STVKES LP LPYL
Sbjct: 141 NAVFLETFGPGSETSSATSEWALAELIKNPHILAKVRQELDQVVGKSTVKESDLPHLPYL 200
Query: 474 HACIKEAMRLHPAAPFLLPRRAIESCEIMGYTIPKDCQLLVNAYAIGRDPKAWKDPTTFR 295
ACIKE MRLHPAAPFLLP RA+E+C +MGYTIPKD Q+LVN YAIGRDPK WK+PTTF+
Sbjct: 201 QACIKETMRLHPAAPFLLPHRAVETCPVMGYTIPKDTQVLVNIYAIGRDPKTWKEPTTFK 260
Query: 294 PERFLESDVDYHGSHYQFIPFGAGRRICIGMPLATRTIPLIVGSLVHTYDWSLPNGRGAD 115
PERFLESDVDY+G H++FIPFGAGRR C+GMPLATRTIPLIV +LV T DWSLP+G+
Sbjct: 261 PERFLESDVDYYGKHFEFIPFGAGRRQCVGMPLATRTIPLIVSNLVQTLDWSLPDGKLPQ 320
Query: 114 ALEMKENLTLSLAIDPSLCIVPNSR 40
L M E+L+LSLA+DP+L ++P SR
Sbjct: 321 DLVMNESLSLSLALDPTLTVIPKSR 345
Score = 82.0 bits (201), Expect(2) = e-132
Identities = 38/61 (62%), Positives = 48/61 (78%)
Frame = -3
Query: 1025 MLQAQSHVREERVGELMDYLRSKQGQTVKIGKFVFGTLLNILGNAVFSKNVFVYSDDDGG 846
MLQ Q HVRE++V EL+++L+ K+GQ VKI + VFGTLLNILGN +FSK+VF Y DG
Sbjct: 1 MLQVQEHVREQKVAELVEFLKGKEGQVVKISQLVFGTLLNILGNVIFSKDVFRYVGADGD 60
Query: 845 K 843
K
Sbjct: 61 K 61
>ref|XP_010253990.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nelumbo nucifera]
Length = 485
Score = 310 bits (795), Expect(2) = e-128
Identities = 147/265 (55%), Positives = 192/265 (72%), Gaps = 1/265 (0%)
Frame = -2
Query: 831 FPILEGLDLQGLKKKCHDRFVRVMNLWKGTVEERKQKRNEESKDMLDVLLANDFNDAQIN 652
+PIL G D QGL+K R+ W V++R++ + D L VLL + F+D QI+
Sbjct: 220 YPILGGSDFQGLRKASAACVKRMNESWAAIVKQRRKNDDHSKNDFLQVLLDSGFSDPQID 279
Query: 651 ALFLETFGPGSETSSATIEWVMAELIKNPDKMAKVREELDTVVGNST-VKESHLPQLPYL 475
A+ LETFGPGS+TS++TIEW MAEL++NP+K+ KVREELD V+ S VKES LP LPYL
Sbjct: 280 AMLLETFGPGSDTSTSTIEWAMAELLRNPEKLVKVREELDRVIRRSNNVKESDLPNLPYL 339
Query: 474 HACIKEAMRLHPAAPFLLPRRAIESCEIMGYTIPKDCQLLVNAYAIGRDPKAWKDPTTFR 295
HAC+KE +RLHP FLLP RA+E+C++M YTIPK CQL+VN YAIGRD K W+ P +F
Sbjct: 340 HACVKETLRLHPPVTFLLPHRAMETCQMMNYTIPKGCQLMVNTYAIGRDSKTWEKPLSFL 399
Query: 294 PERFLESDVDYHGSHYQFIPFGAGRRICIGMPLATRTIPLIVGSLVHTYDWSLPNGRGAD 115
PERFL S++DY G+ +Q+IPFGAGRRIC G+ LATR + LI+ SL+HT+DWSLP+G D
Sbjct: 400 PERFLNSELDYQGNDFQYIPFGAGRRICPGLSLATRVVRLILASLLHTFDWSLPDGMHPD 459
Query: 114 ALEMKENLTLSLAIDPSLCIVPNSR 40
L+M + L+L D L ++P R
Sbjct: 460 ELDMNDKFGLALQKDIPLVVIPKLR 484
Score = 176 bits (447), Expect(2) = e-128
Identities = 87/170 (51%), Positives = 119/170 (70%), Gaps = 1/170 (0%)
Frame = -3
Query: 1364 NLPPGPHAWPLIGSL-PILFSNPDVPLHITLTNMARTHGPLMTLWLGQQSTVVASSPEAA 1188
NLPPGP +WP+IG+L P L P H+ L+ +A+ GPLM L G + VVASS AA
Sbjct: 27 NLPPGPFSWPIIGTLLPKLKKQP----HVELSKLAQRFGPLMLLKFGVEPVVVASSHVAA 82
Query: 1187 TEILKIHDRACSGRYIRMSFRLKHHIKYSLVWSDCTDYWKLLRKIVRTEIFSPKMLQAQS 1008
E+LK DR SGR+ S R+K +I++S+VW+DCTDYWK++RK+ RTE+FS KML Q+
Sbjct: 83 VEVLKNQDRLLSGRFAPHSVRIKGYIEHSMVWADCTDYWKMVRKVWRTELFSTKMLDIQA 142
Query: 1007 HVREERVGELMDYLRSKQGQTVKIGKFVFGTLLNILGNAVFSKNVFVYSD 858
H REE+V ELM +L K+G+ V +FG++LNILG +FSK+V+ + D
Sbjct: 143 HAREEKVSELMKFLIRKEGEKVNFADVIFGSILNILGALIFSKDVYDFED 192
>ref|XP_010253989.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Nelumbo nucifera]
Length = 486
Score = 308 bits (789), Expect(2) = e-125
Identities = 142/266 (53%), Positives = 192/266 (72%), Gaps = 2/266 (0%)
Frame = -2
Query: 831 FPIL-EGLDLQGLKKKCHDRFVRVMNLWKGTVEERKQKRNEESKDMLDVLLANDFNDAQI 655
+PI +G D QGL+K+ R+ W + ER++ + D+L VLL + F+D QI
Sbjct: 220 YPIFFDGSDFQGLRKESAACVKRMSESWAAIINERRKNNDHTKNDLLQVLLDSGFSDPQI 279
Query: 654 NALFLETFGPGSETSSATIEWVMAELIKNPDKMAKVREELDTVVG-NSTVKESHLPQLPY 478
+A+FLETFGPGS+TS++TIEW +AEL++NP+K+ K+ EELD V+G N+TVK+S LP LPY
Sbjct: 280 DAIFLETFGPGSDTSASTIEWALAELLRNPEKLVKLHEELDRVIGRNNTVKDSDLPNLPY 339
Query: 477 LHACIKEAMRLHPAAPFLLPRRAIESCEIMGYTIPKDCQLLVNAYAIGRDPKAWKDPTTF 298
LHAC+KE +RLHP PFL+P A+ESCE+M YTIPK ++LVN YAIGRDP W +P +F
Sbjct: 340 LHACVKETLRLHPPVPFLIPHIALESCEVMNYTIPKGSEVLVNLYAIGRDPTTWDNPNSF 399
Query: 297 RPERFLESDVDYHGSHYQFIPFGAGRRICIGMPLATRTIPLIVGSLVHTYDWSLPNGRGA 118
PERFL S+VDY G+H+Q+IPFGAGRR+C GM L TR + LI+ +LVHT+DWSLP G
Sbjct: 400 LPERFLNSEVDYQGNHFQYIPFGAGRRMCPGMSLGTRVVRLILAALVHTFDWSLPGGMHQ 459
Query: 117 DALEMKENLTLSLAIDPSLCIVPNSR 40
D L+M + + + L ++P R
Sbjct: 460 DELDMADRFGVGFQKETPLVVIPTLR 485
Score = 170 bits (431), Expect(2) = e-125
Identities = 83/169 (49%), Positives = 120/169 (71%), Gaps = 1/169 (0%)
Frame = -3
Query: 1361 LPPGPHAWPLIGS-LPILFSNPDVPLHITLTNMARTHGPLMTLWLGQQSTVVASSPEAAT 1185
LPPGP +WP+IG+ LP +P + ++ L +A+ +GPLM G ++ VVAS+ AA
Sbjct: 28 LPPGPFSWPIIGTQLP----SPTMKPNLELFKLAQRYGPLMLFRFGFENVVVASNHVAAM 83
Query: 1184 EILKIHDRACSGRYIRMSFRLKHHIKYSLVWSDCTDYWKLLRKIVRTEIFSPKMLQAQSH 1005
E+LK DR SGR+ S R+K +I+YS+VW+DCTDYWK++RKI+RTE+FS KML +H
Sbjct: 84 EVLKNQDRVLSGRFKANSVRVKGYIEYSMVWADCTDYWKMVRKILRTELFSTKMLDVHAH 143
Query: 1004 VREERVGELMDYLRSKQGQTVKIGKFVFGTLLNILGNAVFSKNVFVYSD 858
VREE+V ELM +LR K+G+ V +FG +LN+LG ++SK+V+ + D
Sbjct: 144 VREEKVSELMKFLRRKEGEEVNFVDVIFGCILNMLGALIYSKDVYDFED 192
>sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2; AltName: Full=Cytochrome P450 80B2 [Coptis japonica]
gi|9971208|dbj|BAB12433.1|
(S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
Length = 488
Score = 288 bits (736), Expect(2) = e-125
Identities = 141/269 (52%), Positives = 188/269 (69%), Gaps = 1/269 (0%)
Frame = -2
Query: 834 FFPILEGLDLQGLKKKCHDRFVRVMNLWKGTVEERKQKRNEESK-DMLDVLLANDFNDAQ 658
+FPI+ LDL G ++ + ++ ++W ++ER+ + ESK D +DVLL +D +
Sbjct: 219 YFPIMGKLDLFGQRRAVAEVLQQIYDVWGVMLKERRGTKGSESKNDFVDVLLNAGLDDQK 278
Query: 657 INALFLETFGPGSETSSATIEWVMAELIKNPDKMAKVREELDTVVGNSTVKESHLPQLPY 478
INAL +E FG G+ETS++TIEW + EL K P ++K+R EL VVG++TVKES LP LPY
Sbjct: 279 INALLMELFGAGTETSASTIEWAITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPY 338
Query: 477 LHACIKEAMRLHPAAPFLLPRRAIESCEIMGYTIPKDCQLLVNAYAIGRDPKAWKDPTTF 298
L A +KE +RLHP P LLPRRA+E+C +M YTIPK+CQ++VNA+AIGRDPK W DP F
Sbjct: 339 LQAFVKETLRLHPPTPLLLPRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNF 398
Query: 297 RPERFLESDVDYHGSHYQFIPFGAGRRICIGMPLATRTIPLIVGSLVHTYDWSLPNGRGA 118
+PERFL SDVDY G+ ++ IPFG GRRIC G+PLA++ LIV +LV ++WSLP G
Sbjct: 399 KPERFLSSDVDYKGNDFELIPFGGGRRICPGLPLASQFSNLIVATLVQNFEWSLPQGMST 458
Query: 117 DALEMKENLTLSLAIDPSLCIVPNSRA*N 31
L M E L+L DP L IV +RA N
Sbjct: 459 SELSMDEKFGLTLQKDPPLLIVLKARASN 487
Score = 189 bits (480), Expect(2) = e-125
Identities = 92/183 (50%), Positives = 124/183 (67%)
Frame = -3
Query: 1364 NLPPGPHAWPLIGSLPILFSNPDVPLHITLTNMARTHGPLMTLWLGQQSTVVASSPEAAT 1185
NLPPGP P++G+L L P H +A+ +G L +L LG Q+ VVASSP AA
Sbjct: 28 NLPPGPRPSPIVGNLLQLGDKP----HAEFAKLAQKYGELFSLKLGSQTVVVASSPAAAA 83
Query: 1184 EILKIHDRACSGRYIRMSFRLKHHIKYSLVWSDCTDYWKLLRKIVRTEIFSPKMLQAQSH 1005
EILK HD+ SGRY+ SFR+K H++ S+VWS+C D WKLLRK+ RTE+F+PKM+++QS
Sbjct: 84 EILKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDNWKLLRKVCRTELFTPKMIESQSE 143
Query: 1004 VREERVGELMDYLRSKQGQTVKIGKFVFGTLLNILGNAVFSKNVFVYSDDDGGKDVXFFR 825
+RE + E++ +LR K+G+ VKI + VFGTL+NI GN +FSK+VF D GG
Sbjct: 144 IREAKAREMVKFLRGKEGEVVKIVEVVFGTLVNIFGNLIFSKDVFDLEDPTGGSVELKEH 203
Query: 824 YWK 816
WK
Sbjct: 204 LWK 206
>gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
Length = 488
Score = 286 bits (732), Expect(2) = e-124
Identities = 139/266 (52%), Positives = 185/266 (69%), Gaps = 1/266 (0%)
Frame = -2
Query: 834 FFPILEGLDLQGLKKKCHDRFVRVMNLWKGTVEERK-QKRNEESKDMLDVLLANDFNDAQ 658
+FPI+ DL G ++ D ++ ++W ++ER+ K +E + D +DVLL D +
Sbjct: 219 YFPIMGKYDLFGQRRAVADVLQQIYDVWGINLKERRGMKGSESNNDFVDVLLNAGLEDQK 278
Query: 657 INALFLETFGPGSETSSATIEWVMAELIKNPDKMAKVREELDTVVGNSTVKESHLPQLPY 478
INAL +E FG G+ETS++TIEW + EL K P ++K+R EL VVG++TVKES LP LPY
Sbjct: 279 INALLMELFGTGTETSASTIEWAITELTKKPLVVSKIRLELVNVVGDNTVKESDLPHLPY 338
Query: 477 LHACIKEAMRLHPAAPFLLPRRAIESCEIMGYTIPKDCQLLVNAYAIGRDPKAWKDPTTF 298
L A +KE +RLHP P LLPRRA+E+C +M YTIPK+CQ++VNA+AIGRDPK W DP F
Sbjct: 339 LQAFVKETLRLHPPTPLLLPRRALETCTVMNYTIPKECQIMVNAWAIGRDPKTWDDPLNF 398
Query: 297 RPERFLESDVDYHGSHYQFIPFGAGRRICIGMPLATRTIPLIVGSLVHTYDWSLPNGRGA 118
+PERFL SDVDY G+ ++ IPFG GRRIC G+PLA++ I LIV +LV ++WSLP G
Sbjct: 399 KPERFLSSDVDYKGNDFELIPFGGGRRICPGLPLASQFISLIVATLVQNFEWSLPQGMST 458
Query: 117 DALEMKENLTLSLAIDPSLCIVPNSR 40
L M E L+L DP L IV +R
Sbjct: 459 SELSMDEKFGLTLQKDPPLLIVLKAR 484
Score = 188 bits (477), Expect(2) = e-124
Identities = 91/186 (48%), Positives = 126/186 (67%)
Frame = -3
Query: 1373 KRPNLPPGPHAWPLIGSLPILFSNPDVPLHITLTNMARTHGPLMTLWLGQQSTVVASSPE 1194
++ NLPPGP P++G+L L P +A+ +G L +L LG Q+ VVASSP
Sbjct: 25 RQKNLPPGPRPLPIVGNLLQLGDKP----RAEFAKLAQKYGQLFSLKLGSQTVVVASSPA 80
Query: 1193 AATEILKIHDRACSGRYIRMSFRLKHHIKYSLVWSDCTDYWKLLRKIVRTEIFSPKMLQA 1014
AA E+LK HD+ SGRY+ SFR+K H++ S+VWS+C D WKLLRK+ RTE+F+PKM+++
Sbjct: 81 AAAEVLKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDNWKLLRKVCRTELFTPKMIES 140
Query: 1013 QSHVREERVGELMDYLRSKQGQTVKIGKFVFGTLLNILGNAVFSKNVFVYSDDDGGKDVX 834
QS +RE + E++ +LRSK+G+ VKI + VFGTL+NI GN +FSK+VF D GG
Sbjct: 141 QSEIREGKAREMVKFLRSKEGEVVKIVEVVFGTLVNIFGNLIFSKDVFDLEDPTGGSVEL 200
Query: 833 FFRYWK 816
WK
Sbjct: 201 KEHLWK 206
>sp|O64899.1|C80B1_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
AltName: Full=Cytochrome P450 80B1
gi|3127027|gb|AAC39452.1| (S)-N-methylcoclaurine
3'-hydroxylase [Eschscholzia californica]
Length = 487
Score = 287 bits (734), Expect(2) = e-124
Identities = 139/269 (51%), Positives = 179/269 (66%), Gaps = 4/269 (1%)
Frame = -2
Query: 834 FFPILEGLDLQGLKKKCHDRFVRVMNLWKGTVEERKQKRN----EESKDMLDVLLANDFN 667
+FP+L DL G +K+ + + +W ++ERK + + D +DV L + N
Sbjct: 218 YFPMLGKFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYKSKNDFVDVCLDSGLN 277
Query: 666 DAQINALFLETFGPGSETSSATIEWVMAELIKNPDKMAKVREELDTVVGNSTVKESHLPQ 487
D QINAL +E FG G+ETS++TIEW M EL KNP AK+R E+ TVVG +VKES P
Sbjct: 278 DYQINALLMELFGAGTETSASTIEWAMTELTKNPKITAKIRSEIQTVVGERSVKESDFPN 337
Query: 486 LPYLHACIKEAMRLHPAAPFLLPRRAIESCEIMGYTIPKDCQLLVNAYAIGRDPKAWKDP 307
LPYL A +KE +RLHP P LLPRRA+E+C I+ YTIPKDCQ++VNA+ IGRDPK W DP
Sbjct: 338 LPYLEATVKETLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWTDP 397
Query: 306 TTFRPERFLESDVDYHGSHYQFIPFGAGRRICIGMPLATRTIPLIVGSLVHTYDWSLPNG 127
TF PERFL S VD+ G+ + IPFGAGRRIC G+P+A + I L+V + V DW LPNG
Sbjct: 398 LTFSPERFLNSSVDFRGNDFSLIPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNG 457
Query: 126 RGADALEMKENLTLSLAIDPSLCIVPNSR 40
D L ++E L+L +P L IVP SR
Sbjct: 458 MSVDHLIVEEKFGLTLQKEPPLFIVPKSR 486
Score = 187 bits (475), Expect(2) = e-124
Identities = 87/183 (47%), Positives = 126/183 (68%)
Frame = -3
Query: 1364 NLPPGPHAWPLIGSLPILFSNPDVPLHITLTNMARTHGPLMTLWLGQQSTVVASSPEAAT 1185
NLPPGP WP++G+L L P H +A+T+G + TL +G ++ VVAS+ AA+
Sbjct: 27 NLPPGPKPWPIVGNLLQLGEKP----HAQFAELAQTYGDIFTLKMGTETVVVASTSSAAS 82
Query: 1184 EILKIHDRACSGRYIRMSFRLKHHIKYSLVWSDCTDYWKLLRKIVRTEIFSPKMLQAQSH 1005
EILK HDR S RY+ SFR+K H++ S+VWSDCT+ WK LRK+ RTE+F+ KM+++Q+H
Sbjct: 83 EILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWKNLRKVCRTELFTQKMIESQAH 142
Query: 1004 VREERVGELMDYLRSKQGQTVKIGKFVFGTLLNILGNAVFSKNVFVYSDDDGGKDVXFFR 825
VRE++ E+++YL KQG+ VKI + +FGTL+NI GN +FS+N+F D + G
Sbjct: 143 VREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQNIFELGDPNSGSSEFKEY 202
Query: 824 YWK 816
W+
Sbjct: 203 LWR 205
>gb|AKH61487.1| NMCH CYP80B1 P450 N-methylcoclaurine hydroxylase from Eschscholzia
californica [Expression vector pWCD2353]
Length = 490
Score = 286 bits (733), Expect(2) = e-124
Identities = 140/269 (52%), Positives = 179/269 (66%), Gaps = 4/269 (1%)
Frame = -2
Query: 834 FFPILEGLDLQGLKKKCHDRFVRVMNLWKGTVEERKQKRN----EESKDMLDVLLANDFN 667
+FP+L DL G +K+ + + +W ++ERK + + D +DV L + N
Sbjct: 219 YFPMLGKFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLN 278
Query: 666 DAQINALFLETFGPGSETSSATIEWVMAELIKNPDKMAKVREELDTVVGNSTVKESHLPQ 487
D QINAL +E FG G+ETS++TIEW M EL KNP AK+R EL TVVG +VKES P
Sbjct: 279 DYQINALLMELFGAGTETSASTIEWAMTELTKNPKITAKLRSELQTVVGERSVKESDFPN 338
Query: 486 LPYLHACIKEAMRLHPAAPFLLPRRAIESCEIMGYTIPKDCQLLVNAYAIGRDPKAWKDP 307
LPYL A +KE +RLHP P LLPRRA+E+C I+ YTIPKDCQ++VNA+ IGRDPK W DP
Sbjct: 339 LPYLEATVKETLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDP 398
Query: 306 TTFRPERFLESDVDYHGSHYQFIPFGAGRRICIGMPLATRTIPLIVGSLVHTYDWSLPNG 127
TF PERFL S VD+ G+ + IPFGAGRRIC G+P+A + I L+V + V DW LPNG
Sbjct: 399 LTFSPERFLNSSVDFRGNDFSLIPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNG 458
Query: 126 RGADALEMKENLTLSLAIDPSLCIVPNSR 40
D L ++E L+L +P L IVP SR
Sbjct: 459 MSVDHLIVEEKFGLTLQKEPPLFIVPKSR 487
Score = 187 bits (475), Expect(2) = e-124
Identities = 87/183 (47%), Positives = 126/183 (68%)
Frame = -3
Query: 1364 NLPPGPHAWPLIGSLPILFSNPDVPLHITLTNMARTHGPLMTLWLGQQSTVVASSPEAAT 1185
NLPPGP WP++G+L L P H +A+T+G + TL +G ++ VVAS+ AA+
Sbjct: 28 NLPPGPKPWPIVGNLLQLGEKP----HAQFAELAQTYGDIFTLKMGTETVVVASTSSAAS 83
Query: 1184 EILKIHDRACSGRYIRMSFRLKHHIKYSLVWSDCTDYWKLLRKIVRTEIFSPKMLQAQSH 1005
EILK HDR S RY+ SFR+K H++ S+VWSDCT+ WK LRK+ RTE+F+ KM+++Q+H
Sbjct: 84 EILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWKNLRKVCRTELFTQKMIESQAH 143
Query: 1004 VREERVGELMDYLRSKQGQTVKIGKFVFGTLLNILGNAVFSKNVFVYSDDDGGKDVXFFR 825
VRE++ E+++YL KQG+ VKI + +FGTL+NI GN +FS+N+F D + G
Sbjct: 144 VREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQNIFELGDPNSGSSEFKEY 203
Query: 824 YWK 816
W+
Sbjct: 204 LWR 206
>sp|O64900.1|C80B2_ESCCA RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 2;
AltName: Full=Cytochrome P450 80B2
gi|3127029|gb|AAC39453.1| (S)-N-methylcoclaurine
3'-hydroxylase [Eschscholzia californica]
Length = 488
Score = 286 bits (733), Expect(2) = e-123
Identities = 140/269 (52%), Positives = 179/269 (66%), Gaps = 4/269 (1%)
Frame = -2
Query: 834 FFPILEGLDLQGLKKKCHDRFVRVMNLWKGTVEERKQKRN----EESKDMLDVLLANDFN 667
+FP+L DL G +K+ + + +W ++ERK + + D +DV L + N
Sbjct: 219 YFPMLGKFDLFGQRKEVAECLKGIYAIWGAMLQERKLAKKVDGYQSKNDFVDVCLDSGLN 278
Query: 666 DAQINALFLETFGPGSETSSATIEWVMAELIKNPDKMAKVREELDTVVGNSTVKESHLPQ 487
D QINAL +E FG G+ETS++TIEW M EL KNP AK+R EL TVVG +VKES P
Sbjct: 279 DYQINALLMELFGAGTETSASTIEWAMTELTKNPKITAKLRSELQTVVGERSVKESDFPN 338
Query: 486 LPYLHACIKEAMRLHPAAPFLLPRRAIESCEIMGYTIPKDCQLLVNAYAIGRDPKAWKDP 307
LPYL A +KE +RLHP P LLPRRA+E+C I+ YTIPKDCQ++VNA+ IGRDPK W DP
Sbjct: 339 LPYLEATVKETLRLHPPTPLLLPRRALETCTILNYTIPKDCQIMVNAWGIGRDPKTWIDP 398
Query: 306 TTFRPERFLESDVDYHGSHYQFIPFGAGRRICIGMPLATRTIPLIVGSLVHTYDWSLPNG 127
TF PERFL S VD+ G+ + IPFGAGRRIC G+P+A + I L+V + V DW LPNG
Sbjct: 399 LTFSPERFLNSSVDFRGNDFSLIPFGAGRRICPGLPIANQFIALLVATFVQNLDWCLPNG 458
Query: 126 RGADALEMKENLTLSLAIDPSLCIVPNSR 40
D L ++E L+L +P L IVP SR
Sbjct: 459 MSVDHLIVEEKFGLTLQKEPPLFIVPKSR 487
Score = 184 bits (468), Expect(2) = e-123
Identities = 86/183 (46%), Positives = 125/183 (68%)
Frame = -3
Query: 1364 NLPPGPHAWPLIGSLPILFSNPDVPLHITLTNMARTHGPLMTLWLGQQSTVVASSPEAAT 1185
NLPPGP WP++G+L L P H +A+T+G + TL +G ++ VVAS+ AA+
Sbjct: 28 NLPPGPKPWPIVGNLLQLGEKP----HAQFAELAQTYGDIFTLKMGTETVVVASTSSAAS 83
Query: 1184 EILKIHDRACSGRYIRMSFRLKHHIKYSLVWSDCTDYWKLLRKIVRTEIFSPKMLQAQSH 1005
EILK HDR S RY+ SFR+K H++ S+VWSDCT+ WK LRK+ RTE+F+ KM+++Q+H
Sbjct: 84 EILKTHDRILSARYVFQSFRVKGHVENSIVWSDCTETWKNLRKVCRTELFTQKMIESQAH 143
Query: 1004 VREERVGELMDYLRSKQGQTVKIGKFVFGTLLNILGNAVFSKNVFVYSDDDGGKDVXFFR 825
VRE++ E+++YL KQG+ VKI + +FGTL+NI GN +FS+N+F + G
Sbjct: 144 VREKKCEEMVEYLMKKQGEEVKIVEVIFGTLVNIFGNLIFSQNIFELGXPNSGSSEFKEY 203
Query: 824 YWK 816
W+
Sbjct: 204 LWR 206
>sp|Q9SP06.1|C80B3_PAPSO RecName: Full=(S)-N-methylcoclaurine 3'-hydroxylase isozyme 1;
AltName: Full=Cytochrome P450 80B1; AltName:
Full=Cytochrome P450 80B3
gi|6176562|gb|AAF05621.1|AF191772_1
(S)-N-methylcoclaurine 3'-hydroxylase [Papaver
somniferum]
Length = 481
Score = 282 bits (722), Expect(2) = e-120
Identities = 136/268 (50%), Positives = 181/268 (67%), Gaps = 3/268 (1%)
Frame = -2
Query: 834 FFPILEGLDLQGLKKKCHDRFVRVMNLWKGTVEERK---QKRNEESKDMLDVLLANDFND 664
+FP L DL G K D + ++W ++ERK Q N + D +++LL + +D
Sbjct: 213 YFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLKERKIAKQHNNSKKNDFVEILLDSGLDD 272
Query: 663 AQINALFLETFGPGSETSSATIEWVMAELIKNPDKMAKVREELDTVVGNSTVKESHLPQL 484
QINAL +E FG G+ETS++TIEW ++EL KNP A +R EL +VVG VKES +P +
Sbjct: 273 QQINALLMEIFGAGTETSASTIEWALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNM 332
Query: 483 PYLHACIKEAMRLHPAAPFLLPRRAIESCEIMGYTIPKDCQLLVNAYAIGRDPKAWKDPT 304
PYL A +KE +RLHPA P LLPRRA+E+C+++ YTIPK+CQ++VNA+ IGRDPK W DP
Sbjct: 333 PYLQAFVKETLRLHPATPLLLPRRALETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPL 392
Query: 303 TFRPERFLESDVDYHGSHYQFIPFGAGRRICIGMPLATRTIPLIVGSLVHTYDWSLPNGR 124
F PERFL S +D+ G+ ++ IPFGAGRRIC G+PLAT+ I LIV SLV +DW P G
Sbjct: 393 KFSPERFLNSSIDFKGNDFELIPFGAGRRICPGVPLATQFISLIVSSLVQNFDWGFPKGM 452
Query: 123 GADALEMKENLTLSLAIDPSLCIVPNSR 40
L M+E L+L +P L IVP +R
Sbjct: 453 DPSQLIMEEKFGLTLQKEPPLYIVPKTR 480
Score = 181 bits (459), Expect(2) = e-120
Identities = 84/172 (48%), Positives = 119/172 (69%)
Frame = -3
Query: 1361 LPPGPHAWPLIGSLPILFSNPDVPLHITLTNMARTHGPLMTLWLGQQSTVVASSPEAATE 1182
LPPGP WP++G+L L P H +A T+G L +L LG ++ VVAS+P AA+E
Sbjct: 23 LPPGPKPWPIVGNLLQLGEKP----HSQFAQLAETYGDLFSLKLGSETVVVASTPLAASE 78
Query: 1181 ILKIHDRACSGRYIRMSFRLKHHIKYSLVWSDCTDYWKLLRKIVRTEIFSPKMLQAQSHV 1002
ILK HDR SGRY+ SFR+K H++ S+VWS+C + WK LRK+ RTE+F+ KM+++Q+ V
Sbjct: 79 ILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTELFTQKMIESQAEV 138
Query: 1001 REERVGELMDYLRSKQGQTVKIGKFVFGTLLNILGNAVFSKNVFVYSDDDGG 846
RE + E+++YL+ G VKI + VFGTL+NI GN +FS+N+F D+ G
Sbjct: 139 RESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSG 190
>gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [Papaver bracteatum]
Length = 486
Score = 282 bits (722), Expect(2) = e-120
Identities = 135/268 (50%), Positives = 184/268 (68%), Gaps = 3/268 (1%)
Frame = -2
Query: 834 FFPILEGLDLQGLKKKCHDRFVRVMNLWKGTVEERKQKR---NEESKDMLDVLLANDFND 664
+FP L DL G +K D + ++W ++ERK + N + D +++LL + +D
Sbjct: 218 YFPFLGRFDLFGQRKDVADCLQGIYSVWGAMLKERKIAKLHNNSKKNDFVEILLDSGLDD 277
Query: 663 AQINALFLETFGPGSETSSATIEWVMAELIKNPDKMAKVREELDTVVGNSTVKESHLPQL 484
QINAL +E FG G+ETS++TIEW ++EL KNP+ A +R EL +VVG VKES +P +
Sbjct: 278 QQINALLMEIFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVGKRPVKESDIPNM 337
Query: 483 PYLHACIKEAMRLHPAAPFLLPRRAIESCEIMGYTIPKDCQLLVNAYAIGRDPKAWKDPT 304
PYL A +KE +RLHPA P LLPRRA+E+C+++ YTIPK+CQ++VNA+ IGRDPK W DP
Sbjct: 338 PYLQAFVKETLRLHPATPLLLPRRALETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPL 397
Query: 303 TFRPERFLESDVDYHGSHYQFIPFGAGRRICIGMPLATRTIPLIVGSLVHTYDWSLPNGR 124
F PERFL S +D+ G+ ++ IPFGAGRRIC G+PLAT+ I LIV +LV +DW LP G
Sbjct: 398 KFAPERFLNSSIDFKGNDFELIPFGAGRRICPGVPLATQFISLIVPTLVQNFDWGLPKGM 457
Query: 123 GADALEMKENLTLSLAIDPSLCIVPNSR 40
L M+E L+L +P L IVP +R
Sbjct: 458 DPSQLIMEEKFGLTLQKEPPLYIVPKNR 485
Score = 181 bits (458), Expect(2) = e-120
Identities = 83/172 (48%), Positives = 120/172 (69%)
Frame = -3
Query: 1361 LPPGPHAWPLIGSLPILFSNPDVPLHITLTNMARTHGPLMTLWLGQQSTVVASSPEAATE 1182
LPPGP WP++G+L L P H +A+T+G L TL LG ++ VVAS+P AA+E
Sbjct: 28 LPPGPKPWPIVGNLLQLGEKP----HSQFAELAKTYGDLFTLKLGSETVVVASTPLAASE 83
Query: 1181 ILKIHDRACSGRYIRMSFRLKHHIKYSLVWSDCTDYWKLLRKIVRTEIFSPKMLQAQSHV 1002
ILK HDR SGRY+ SFR+K H++ S+VWS+C + WK LRK+ R E+F+ KM+++Q+ +
Sbjct: 84 ILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRAELFTQKMIESQAEI 143
Query: 1001 REERVGELMDYLRSKQGQTVKIGKFVFGTLLNILGNAVFSKNVFVYSDDDGG 846
RE + E++++L+ QG VKI + VFGTL+NI GN +FS+N+F D+ G
Sbjct: 144 RESKAMEMVEFLKRNQGSEVKIVEVVFGTLVNIFGNLIFSQNIFKLGDESSG 195
>gb|AAF61400.1|AF134590_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
Length = 481
Score = 281 bits (720), Expect(2) = e-120
Identities = 136/268 (50%), Positives = 181/268 (67%), Gaps = 3/268 (1%)
Frame = -2
Query: 834 FFPILEGLDLQGLKKKCHDRFVRVMNLWKGTVEERK---QKRNEESKDMLDVLLANDFND 664
+FP L DL G K D + ++W ++E K Q N + D +++LL + +D
Sbjct: 213 YFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLKESKIAKQHNNSKKNDFVEILLDSGLDD 272
Query: 663 AQINALFLETFGPGSETSSATIEWVMAELIKNPDKMAKVREELDTVVGNSTVKESHLPQL 484
QINAL +E FG G+ETS++TIEW ++EL KNP A +R EL +VVG VKES +P +
Sbjct: 273 QQINALLMEIFGAGTETSASTIEWALSELTKNPQVTANMRLELLSVVGKRPVKESDIPNM 332
Query: 483 PYLHACIKEAMRLHPAAPFLLPRRAIESCEIMGYTIPKDCQLLVNAYAIGRDPKAWKDPT 304
PYL A +KE +RLHPA P LLPRRA+E+C+++ YTIPK+CQ++VNA+ IGRDPK W DP
Sbjct: 333 PYLQAFVKETLRLHPATPLLLPRRALETCKVLNYTIPKECQIMVNAWGIGRDPKRWTDPL 392
Query: 303 TFRPERFLESDVDYHGSHYQFIPFGAGRRICIGMPLATRTIPLIVGSLVHTYDWSLPNGR 124
F PERFL S +D+ G+ ++ IPFGAGRRIC G+PLAT+ I LIV SLV +DW LP G
Sbjct: 393 KFSPERFLNSSIDFKGNDFELIPFGAGRRICPGVPLATQFISLIVSSLVQNFDWGLPKGM 452
Query: 123 GADALEMKENLTLSLAIDPSLCIVPNSR 40
L M+E L+L +P L IVP +R
Sbjct: 453 DPSQLIMEEKFGLTLQKEPPLYIVPKTR 480
Score = 180 bits (456), Expect(2) = e-120
Identities = 83/172 (48%), Positives = 119/172 (69%)
Frame = -3
Query: 1361 LPPGPHAWPLIGSLPILFSNPDVPLHITLTNMARTHGPLMTLWLGQQSTVVASSPEAATE 1182
LPPGP WP++G+L L P H +A T+G L +L LG ++ VVAS+P AA+E
Sbjct: 23 LPPGPKPWPIVGNLLQLGEKP----HSQFAQLAETYGDLFSLKLGSETVVVASTPLAASE 78
Query: 1181 ILKIHDRACSGRYIRMSFRLKHHIKYSLVWSDCTDYWKLLRKIVRTEIFSPKMLQAQSHV 1002
ILK HDR SGRY+ SFR+K H++ S+VWS+C + WK LRK+ RT++F+ KM+++Q+ V
Sbjct: 79 ILKTHDRVLSGRYVFQSFRVKEHVENSIVWSECNETWKKLRKVCRTDLFTQKMIESQAEV 138
Query: 1001 REERVGELMDYLRSKQGQTVKIGKFVFGTLLNILGNAVFSKNVFVYSDDDGG 846
RE + E+++YL+ G VKI + VFGTL+NI GN +FS+N+F D+ G
Sbjct: 139 RESKAMEMVEYLKKNVGNEVKIAEVVFGTLVNIFGNLIFSQNIFKLGDESSG 190
>emb|CBI31830.3| unnamed protein product [Vitis vinifera]
Length = 1538
Score = 300 bits (769), Expect(2) = e-118
Identities = 136/267 (50%), Positives = 196/267 (73%), Gaps = 2/267 (0%)
Frame = -2
Query: 834 FFPILEGLDLQGLKKKCHDRFVRVMNLWKGTVEERKQKRNEES--KDMLDVLLANDFNDA 661
F+PIL+GL+L G KKK + R++ W+GT++ER++++N S +D+L+ L F D
Sbjct: 1271 FYPILDGLNLHGSKKKSKEALGRILATWEGTLKERRKQKNPGSSHRDLLEAFLEIRFEDD 1330
Query: 660 QINALFLETFGPGSETSSATIEWVMAELIKNPDKMAKVREELDTVVGNSTVKESHLPQLP 481
QIN + LE F G++TS+ TIEW + +LI+NPD M K+R+EL ++G S V+ESHLP LP
Sbjct: 1331 QINQVILELFSAGADTSTLTIEWAITQLIRNPDVMYKLRDELTKIIGESPVRESHLPHLP 1390
Query: 480 YLHACIKEAMRLHPAAPFLLPRRAIESCEIMGYTIPKDCQLLVNAYAIGRDPKAWKDPTT 301
YL AC+KE +RLHP AP LLP RA+E+C++MGYTIPKD Q+ VN +A+GRDPK W DP +
Sbjct: 1391 YLQACVKETLRLHPPAPLLLPHRAMETCQVMGYTIPKDSQVFVNIWAMGRDPKVWDDPLS 1450
Query: 300 FRPERFLESDVDYHGSHYQFIPFGAGRRICIGMPLATRTIPLIVGSLVHTYDWSLPNGRG 121
F PERFL+S +++ G+ +++IPFGAGRRIC GM L R +PL++ +LVH +DWSLP+
Sbjct: 1451 FTPERFLDSKLEFKGNDFEYIPFGAGRRICPGMALGARQVPLVLATLVHLFDWSLPDNMD 1510
Query: 120 ADALEMKENLTLSLAIDPSLCIVPNSR 40
+ ++M+E L ++L + L +VP R
Sbjct: 1511 SAQIDMEEWLVITLRKENPLRLVPKVR 1537
Score = 155 bits (392), Expect(2) = e-118
Identities = 84/186 (45%), Positives = 120/186 (64%), Gaps = 4/186 (2%)
Frame = -3
Query: 1376 LKRPNLPPGPHAWPLIGSLPILFSNPDVPLHITLTNMARTHGPLMTLWLGQQSTVVASSP 1197
LK P LPPGP+ WP+IG+L L NP H+ L ++A+ HGPLM+L LG Q VVASSP
Sbjct: 1078 LKPPPLPPGPYPWPIIGNLLQLGKNP----HVKLASLAKLHGPLMSLRLGTQLMVVASSP 1133
Query: 1196 EAATEILKIHDRACSGRYIRMSFRLK----HHIKYSLVWSDCTDYWKLLRKIVRTEIFSP 1029
AA E+LK HDR SGRY+ S +K +H+ + +CT+ WK LR I RTE+FS
Sbjct: 1134 AAALEVLKTHDRTLSGRYVSSSLSVKDPKLNHLSLAFA-KECTNNWKNLRTICRTEMFSG 1192
Query: 1028 KMLQAQSHVREERVGELMDYLRSKQGQTVKIGKFVFGTLLNILGNAVFSKNVFVYSDDDG 849
K +++ +RE +V EL+++L +K+G+ VK+ VF T+ NIL N FS ++ + + +G
Sbjct: 1193 KAMESHVELRERKVMELVEFLATKEGEVVKVMDLVFTTICNILSNTFFSMDLCDF-EREG 1251
Query: 848 GKDVXF 831
KD +
Sbjct: 1252 LKDFIY 1257
Score = 293 bits (750), Expect(2) = e-116
Identities = 137/267 (51%), Positives = 189/267 (70%), Gaps = 2/267 (0%)
Frame = -2
Query: 834 FFPILEGLDLQGLKKKCHDRFVRVMNLWKGTVEERKQKRNEES--KDMLDVLLANDFNDA 661
++PIL GLD+QG+ +K + F R+ W+ ++ER+ +R+ S +D L+ LL F D
Sbjct: 771 YYPILGGLDIQGINRKAKEMFERIPTTWEDILKERRTQRSNRSSHRDFLEALLEIGFEDD 830
Query: 660 QINALFLETFGPGSETSSATIEWVMAELIKNPDKMAKVREELDTVVGNSTVKESHLPQLP 481
QIN + LE F G+ETSS T+EW MAELI+N D M K+R EL +VG S V+ESHLP+LP
Sbjct: 831 QINQVILELFSAGAETSSLTVEWAMAELIRNQDAMDKLRGELRQIVGESPVRESHLPRLP 890
Query: 480 YLHACIKEAMRLHPAAPFLLPRRAIESCEIMGYTIPKDCQLLVNAYAIGRDPKAWKDPTT 301
YL AC+KEA+RLHP AP LLP A E+C++MGYTIPKD Q+ VN +A+ RDPK W DP +
Sbjct: 891 YLQACVKEALRLHPPAPLLLPHLAAETCQVMGYTIPKDSQIFVNIWAMARDPKIWDDPLS 950
Query: 300 FRPERFLESDVDYHGSHYQFIPFGAGRRICIGMPLATRTIPLIVGSLVHTYDWSLPNGRG 121
F+PERFL+S +D+ G+ +++IPFGAGRRIC G+ L R +PLI+ + VH + WSLP
Sbjct: 951 FKPERFLDSKLDFKGNDFEYIPFGAGRRICPGLALGGRQVPLILATFVHLFGWSLPGNMD 1010
Query: 120 ADALEMKENLTLSLAIDPSLCIVPNSR 40
+ L+M+E L ++L + L +VP R
Sbjct: 1011 SAQLDMEEWLVITLRKEQPLRLVPRVR 1037
Score = 155 bits (392), Expect(2) = e-116
Identities = 85/179 (47%), Positives = 118/179 (65%), Gaps = 5/179 (2%)
Frame = -3
Query: 1376 LKRPNLPPGPHAWPLIGSLPILFSNPDVPLHITLTNMARTHGPLMTLWLGQQSTVVASSP 1197
LK P LPPGP+ WP+IG+L + NP H L N+A+ HGPLM+L LG Q VVASSP
Sbjct: 573 LKPPPLPPGPYPWPIIGNLLQMGKNP----HAKLANLAKLHGPLMSLRLGTQLMVVASSP 628
Query: 1196 EAATEILKIHDRACSGRYIRMSFRLKH----HIKYSLVWS-DCTDYWKLLRKIVRTEIFS 1032
AA E+LK HDRA SGRY+ S +K+ H+ S+V++ DC WK LR I R E+FS
Sbjct: 629 AAAMEVLKTHDRALSGRYLSSSVPVKNPKLNHL--SIVFAKDCNTNWKNLRAICRMELFS 686
Query: 1031 PKMLQAQSHVREERVGELMDYLRSKQGQTVKIGKFVFGTLLNILGNAVFSKNVFVYSDD 855
K +++Q +RE +V EL+++L +K+G+ VK+ VF T+ NIL N FS ++ + D+
Sbjct: 687 GKAMESQVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDE 745
Score = 282 bits (721), Expect(2) = e-107
Identities = 130/254 (51%), Positives = 178/254 (70%), Gaps = 2/254 (0%)
Frame = -2
Query: 834 FFPILEGLDLQGLKKKCHDRFVRVMNLWKGTVEERKQKRNEESK--DMLDVLLANDFNDA 661
F+PIL GLD+QG++KK + F R+ W+ ++ER+++R S D LD LL F D
Sbjct: 230 FYPILGGLDIQGIRKKLKEIFRRIPTTWEDILKERRKQRIHGSSHGDFLDALLETGFEDD 289
Query: 660 QINALFLETFGPGSETSSATIEWVMAELIKNPDKMAKVREELDTVVGNSTVKESHLPQLP 481
QIN + +E F G ETSS T+EW MAELIKN D M K+ EL ++G S V+ESHLP LP
Sbjct: 290 QINHVIMELFFAGPETSSLTVEWAMAELIKNQDAMHKLCNELTQIIGESPVRESHLPHLP 349
Query: 480 YLHACIKEAMRLHPAAPFLLPRRAIESCEIMGYTIPKDCQLLVNAYAIGRDPKAWKDPTT 301
YL AC+KE +RLHP P LLP RA E+C+IMGYTIPKD + VN +A+GRDP W+DP +
Sbjct: 350 YLQACVKETLRLHPTGPLLLPHRATETCQIMGYTIPKDSIIFVNMWAMGRDPGTWEDPLS 409
Query: 300 FRPERFLESDVDYHGSHYQFIPFGAGRRICIGMPLATRTIPLIVGSLVHTYDWSLPNGRG 121
F+PERFL+S +++ G+ +++IPFGAGRR+C GMPLA R +P+I+ + V +DWS P
Sbjct: 410 FKPERFLDSKLEFKGNDFEYIPFGAGRRMCPGMPLAARLVPMILATFVRLFDWSTPGDMD 469
Query: 120 ADALEMKENLTLSL 79
++M+E ++L
Sbjct: 470 FAEIDMEERFVITL 483
Score = 138 bits (347), Expect(2) = e-107
Identities = 76/180 (42%), Positives = 111/180 (61%), Gaps = 4/180 (2%)
Frame = -3
Query: 1376 LKRPNLPPGPHAWPLIGSLPILFSNPDVPLHITLTNMARTHGPLMTLWLGQQSTVVASSP 1197
LK P LPPGP+ WP+IG+L + N LH+ L N+A+ HGPLM+L LG Q VVASS
Sbjct: 33 LKSPPLPPGPYPWPIIGNLLQMGGN----LHVKLANLAKRHGPLMSLRLGTQIMVVASSS 88
Query: 1196 EAATEILKIHDRACSGRYIRMSFRLK----HHIKYSLVWSDCTDYWKLLRKIVRTEIFSP 1029
AA E+LK HDR SGRY+ + + +H+ + C W+ L+ I R E+FS
Sbjct: 89 AAAMEVLKTHDRTLSGRYVSTTIPVNSPKLNHLAMAFA-KVCNSDWRNLKAICRMELFSG 147
Query: 1028 KMLQAQSHVREERVGELMDYLRSKQGQTVKIGKFVFGTLLNILGNAVFSKNVFVYSDDDG 849
K ++++ +RE +V EL+++L K+G+ VK+ V+ T+ NIL N FS + +SD +G
Sbjct: 148 KAMESRVELRERKVMELVEFLEKKEGEVVKVMDLVYTTVCNILSNKFFSID---FSDFEG 204
>ref|XP_002513347.1| (S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative [Ricinus
communis] gi|223547255|gb|EEF48750.1|
(S)-N-methylcoclaurine 3'-hydroxylase isozyme, putative
[Ricinus communis]
Length = 496
Score = 296 bits (759), Expect(2) = e-118
Identities = 132/267 (49%), Positives = 196/267 (73%), Gaps = 2/267 (0%)
Frame = -2
Query: 834 FFPILEGLDLQGLKKKCHDRFVRVMNLWKGTVEERKQKRNEESK--DMLDVLLANDFNDA 661
F+P+L LD QG+K+K + R+ +W ++ER+++ ++++ D LDV L+N F+D
Sbjct: 229 FYPVLTKLDPQGIKRKMKNCLERMFGVWDIYIKERRERHVKDARKTDFLDVFLSNGFDDH 288
Query: 660 QINALFLETFGPGSETSSATIEWVMAELIKNPDKMAKVREELDTVVGNSTVKESHLPQLP 481
QIN L LE F G++T++ T+EW MAEL+K + KVREEL+T +G ++ESH+PQL
Sbjct: 289 QINWLLLELFSAGADTTTTTVEWAMAELLKEITVLEKVREELETEIGKDMIRESHIPQLK 348
Query: 480 YLHACIKEAMRLHPAAPFLLPRRAIESCEIMGYTIPKDCQLLVNAYAIGRDPKAWKDPTT 301
YL+AC+KE +RLHP PFL+PRRA E+CE+M YTIPK Q++VN +AIGRDP AW+DP +
Sbjct: 349 YLNACVKETLRLHPPVPFLIPRRAPEACEVMNYTIPKHSQIIVNVWAIGRDPSAWEDPLS 408
Query: 300 FRPERFLESDVDYHGSHYQFIPFGAGRRICIGMPLATRTIPLIVGSLVHTYDWSLPNGRG 121
+RPERFL+S++D+ G +++F+PFG+GRRIC G+P+ TR +PLI+ SLVH +DWSL NG
Sbjct: 409 YRPERFLDSNLDFKGHNFEFLPFGSGRRICPGLPMGTRQLPLILASLVHCFDWSLQNGDD 468
Query: 120 ADALEMKENLTLSLAIDPSLCIVPNSR 40
L+M + +++L + L +VP +
Sbjct: 469 PAMLDMNDKFSITLEKEQHLLVVPKRK 495
Score = 159 bits (402), Expect(2) = e-118
Identities = 80/175 (45%), Positives = 119/175 (68%), Gaps = 3/175 (1%)
Frame = -3
Query: 1373 KRPNLPPGPHAWPLIGSLPILFSNPDVPLHITLTNMARTHGPLMTLWLGQQSTVVASSPE 1194
KR LPPGP WP++G++ + NP HI++ N A+ HGPL++L LG + VVASSP
Sbjct: 33 KRRPLPPGPRQWPILGNILQVGKNP----HISMANYAKVHGPLISLRLGTRVVVVASSPT 88
Query: 1193 AATEILKIHDRACSGRYIRMSFRLKHHI--KYSLVWS-DCTDYWKLLRKIVRTEIFSPKM 1023
AA EILK HDR SGRYI + + ++ + +LVW+ C+D WK LR + R+E+FS K
Sbjct: 89 AAAEILKTHDRLLSGRYIPATTPYEDNVLDRIALVWNPSCSDQWKFLRAMCRSELFSAKA 148
Query: 1022 LQAQSHVREERVGELMDYLRSKQGQTVKIGKFVFGTLLNILGNAVFSKNVFVYSD 858
+++Q+ +RE+++ E++D+L SKQGQ V IG+ VF T N + N +FSK++ + D
Sbjct: 149 IESQATLREKKLTEMLDFLTSKQGQIVNIGEVVFTTAFNTISNLLFSKDLLSFED 203
>ref|XP_010662515.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 493
Score = 300 bits (769), Expect(2) = e-118
Identities = 136/267 (50%), Positives = 196/267 (73%), Gaps = 2/267 (0%)
Frame = -2
Query: 834 FFPILEGLDLQGLKKKCHDRFVRVMNLWKGTVEERKQKRNEES--KDMLDVLLANDFNDA 661
F+PIL+GL+L G KKK + R++ W+GT++ER++++N S +D+L+ L F D
Sbjct: 226 FYPILDGLNLHGSKKKSKEALGRILATWEGTLKERRKQKNPGSSHRDLLEAFLEIRFEDD 285
Query: 660 QINALFLETFGPGSETSSATIEWVMAELIKNPDKMAKVREELDTVVGNSTVKESHLPQLP 481
QIN + LE F G++TS+ TIEW + +LI+NPD M K+R+EL ++G S V+ESHLP LP
Sbjct: 286 QINQVILELFSAGADTSTLTIEWAITQLIRNPDVMYKLRDELTKIIGESPVRESHLPHLP 345
Query: 480 YLHACIKEAMRLHPAAPFLLPRRAIESCEIMGYTIPKDCQLLVNAYAIGRDPKAWKDPTT 301
YL AC+KE +RLHP AP LLP RA+E+C++MGYTIPKD Q+ VN +A+GRDPK W DP +
Sbjct: 346 YLQACVKETLRLHPPAPLLLPHRAMETCQVMGYTIPKDSQVFVNIWAMGRDPKVWDDPLS 405
Query: 300 FRPERFLESDVDYHGSHYQFIPFGAGRRICIGMPLATRTIPLIVGSLVHTYDWSLPNGRG 121
F PERFL+S +++ G+ +++IPFGAGRRIC GM L R +PL++ +LVH +DWSLP+
Sbjct: 406 FTPERFLDSKLEFKGNDFEYIPFGAGRRICPGMALGARQVPLVLATLVHLFDWSLPDNMD 465
Query: 120 ADALEMKENLTLSLAIDPSLCIVPNSR 40
+ ++M+E L ++L + L +VP R
Sbjct: 466 SAQIDMEEWLVITLRKENPLRLVPKVR 492
Score = 155 bits (392), Expect(2) = e-118
Identities = 84/186 (45%), Positives = 120/186 (64%), Gaps = 4/186 (2%)
Frame = -3
Query: 1376 LKRPNLPPGPHAWPLIGSLPILFSNPDVPLHITLTNMARTHGPLMTLWLGQQSTVVASSP 1197
LK P LPPGP+ WP+IG+L L NP H+ L ++A+ HGPLM+L LG Q VVASSP
Sbjct: 33 LKPPPLPPGPYPWPIIGNLLQLGKNP----HVKLASLAKLHGPLMSLRLGTQLMVVASSP 88
Query: 1196 EAATEILKIHDRACSGRYIRMSFRLK----HHIKYSLVWSDCTDYWKLLRKIVRTEIFSP 1029
AA E+LK HDR SGRY+ S +K +H+ + +CT+ WK LR I RTE+FS
Sbjct: 89 AAALEVLKTHDRTLSGRYVSSSLSVKDPKLNHLSLAFA-KECTNNWKNLRTICRTEMFSG 147
Query: 1028 KMLQAQSHVREERVGELMDYLRSKQGQTVKIGKFVFGTLLNILGNAVFSKNVFVYSDDDG 849
K +++ +RE +V EL+++L +K+G+ VK+ VF T+ NIL N FS ++ + + +G
Sbjct: 148 KAMESHVELRERKVMELVEFLATKEGEVVKVMDLVFTTICNILSNTFFSMDLCDF-EREG 206
Query: 848 GKDVXF 831
KD +
Sbjct: 207 LKDFIY 212
>ref|XP_002274562.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme 2
[Vitis vinifera]
Length = 498
Score = 293 bits (750), Expect(2) = e-116
Identities = 137/267 (51%), Positives = 189/267 (70%), Gaps = 2/267 (0%)
Frame = -2
Query: 834 FFPILEGLDLQGLKKKCHDRFVRVMNLWKGTVEERKQKRNEES--KDMLDVLLANDFNDA 661
++PIL GLD+QG+ +K + F R+ W+ ++ER+ +R+ S +D L+ LL F D
Sbjct: 231 YYPILGGLDIQGINRKAKEMFERIPTTWEDILKERRTQRSNRSSHRDFLEALLEIGFEDD 290
Query: 660 QINALFLETFGPGSETSSATIEWVMAELIKNPDKMAKVREELDTVVGNSTVKESHLPQLP 481
QIN + LE F G+ETSS T+EW MAELI+N D M K+R EL +VG S V+ESHLP+LP
Sbjct: 291 QINQVILELFSAGAETSSLTVEWAMAELIRNQDAMDKLRGELRQIVGESPVRESHLPRLP 350
Query: 480 YLHACIKEAMRLHPAAPFLLPRRAIESCEIMGYTIPKDCQLLVNAYAIGRDPKAWKDPTT 301
YL AC+KEA+RLHP AP LLP A E+C++MGYTIPKD Q+ VN +A+ RDPK W DP +
Sbjct: 351 YLQACVKEALRLHPPAPLLLPHLAAETCQVMGYTIPKDSQIFVNIWAMARDPKIWDDPLS 410
Query: 300 FRPERFLESDVDYHGSHYQFIPFGAGRRICIGMPLATRTIPLIVGSLVHTYDWSLPNGRG 121
F+PERFL+S +D+ G+ +++IPFGAGRRIC G+ L R +PLI+ + VH + WSLP
Sbjct: 411 FKPERFLDSKLDFKGNDFEYIPFGAGRRICPGLALGGRQVPLILATFVHLFGWSLPGNMD 470
Query: 120 ADALEMKENLTLSLAIDPSLCIVPNSR 40
+ L+M+E L ++L + L +VP R
Sbjct: 471 SAQLDMEEWLVITLRKEQPLRLVPRVR 497
Score = 155 bits (392), Expect(2) = e-116
Identities = 85/179 (47%), Positives = 118/179 (65%), Gaps = 5/179 (2%)
Frame = -3
Query: 1376 LKRPNLPPGPHAWPLIGSLPILFSNPDVPLHITLTNMARTHGPLMTLWLGQQSTVVASSP 1197
LK P LPPGP+ WP+IG+L + NP H L N+A+ HGPLM+L LG Q VVASSP
Sbjct: 33 LKPPPLPPGPYPWPIIGNLLQMGKNP----HAKLANLAKLHGPLMSLRLGTQLMVVASSP 88
Query: 1196 EAATEILKIHDRACSGRYIRMSFRLKH----HIKYSLVWS-DCTDYWKLLRKIVRTEIFS 1032
AA E+LK HDRA SGRY+ S +K+ H+ S+V++ DC WK LR I R E+FS
Sbjct: 89 AAAMEVLKTHDRALSGRYLSSSVPVKNPKLNHL--SIVFAKDCNTNWKNLRAICRMELFS 146
Query: 1031 PKMLQAQSHVREERVGELMDYLRSKQGQTVKIGKFVFGTLLNILGNAVFSKNVFVYSDD 855
K +++Q +RE +V EL+++L +K+G+ VK+ VF T+ NIL N FS ++ + D+
Sbjct: 147 GKAMESQVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDE 205
>emb|CAN62647.1| hypothetical protein VITISV_013219 [Vitis vinifera]
Length = 498
Score = 293 bits (750), Expect(2) = e-116
Identities = 137/267 (51%), Positives = 189/267 (70%), Gaps = 2/267 (0%)
Frame = -2
Query: 834 FFPILEGLDLQGLKKKCHDRFVRVMNLWKGTVEERKQKRNEES--KDMLDVLLANDFNDA 661
++PIL GLD+QG+ +K + F R+ W+ ++ER+ +R+ S +D L+ LL F D
Sbjct: 231 YYPILGGLDIQGINRKAKEMFERIPTTWEDILKERRTQRSNRSSHRDFLEALLEIGFEDD 290
Query: 660 QINALFLETFGPGSETSSATIEWVMAELIKNPDKMAKVREELDTVVGNSTVKESHLPQLP 481
QIN + LE F G+ETSS T+EW MAELI+N D M K+R EL +VG S V+ESHLP+LP
Sbjct: 291 QINQVILELFSAGAETSSLTVEWAMAELIRNQDAMDKLRGELRQIVGESPVRESHLPRLP 350
Query: 480 YLHACIKEAMRLHPAAPFLLPRRAIESCEIMGYTIPKDCQLLVNAYAIGRDPKAWKDPTT 301
YL AC+KEA+RLHP AP LLP A E+C++MGYTIPKD Q+ VN +A+ RDPK W DP +
Sbjct: 351 YLQACVKEALRLHPPAPLLLPHLAAETCQVMGYTIPKDSQIFVNIWAMARDPKIWDDPLS 410
Query: 300 FRPERFLESDVDYHGSHYQFIPFGAGRRICIGMPLATRTIPLIVGSLVHTYDWSLPNGRG 121
F+PERFL+S +D+ G+ +++IPFGAGRRIC G+ L R +PLI+ + VH + WSLP
Sbjct: 411 FKPERFLDSKLDFKGNDFEYIPFGAGRRICPGLALGGRQVPLILATFVHLFGWSLPGNMD 470
Query: 120 ADALEMKENLTLSLAIDPSLCIVPNSR 40
+ L+M+E L ++L + L +VP R
Sbjct: 471 SAQLDMEEWLVITLRKEQPLRLVPRVR 497
Score = 155 bits (392), Expect(2) = e-116
Identities = 85/179 (47%), Positives = 118/179 (65%), Gaps = 5/179 (2%)
Frame = -3
Query: 1376 LKRPNLPPGPHAWPLIGSLPILFSNPDVPLHITLTNMARTHGPLMTLWLGQQSTVVASSP 1197
LK P LPPGP+ WP+IG+L + NP H L N+A+ HGPLM+L LG Q VVASSP
Sbjct: 33 LKPPPLPPGPYPWPIIGNLLQMGKNP----HAKLANLAKLHGPLMSLRLGTQLMVVASSP 88
Query: 1196 EAATEILKIHDRACSGRYIRMSFRLKH----HIKYSLVWS-DCTDYWKLLRKIVRTEIFS 1032
AA E+LK HDRA SGRY+ S +K+ H+ S+V++ DC WK LR I R E+FS
Sbjct: 89 AAAMEVLKTHDRALSGRYLSXSVPVKNPKLNHL--SIVFAKDCNTNWKNLRAICRMELFS 146
Query: 1031 PKMLQAQSHVREERVGELMDYLRSKQGQTVKIGKFVFGTLLNILGNAVFSKNVFVYSDD 855
K +++Q +RE +V EL+++L +K+G+ VK+ VF T+ NIL N FS ++ + D+
Sbjct: 147 GKAMESQVELRERKVTELVEFLATKEGEVVKVMDLVFTTICNILSNKFFSMDLCDFEDE 205
>gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum flavum subsp.
glaucum]
Length = 491
Score = 273 bits (698), Expect(2) = e-116
Identities = 129/265 (48%), Positives = 180/265 (67%)
Frame = -2
Query: 834 FFPILEGLDLQGLKKKCHDRFVRVMNLWKGTVEERKQKRNEESKDMLDVLLANDFNDAQI 655
+FPI+ DL G +++ ++ ++W ++ER+ D +VLL +D +I
Sbjct: 220 YFPIMGKFDLFGQRREVAKVLKQIYDVWGVMLKERRSITGHRENDFANVLLNAGLDDQKI 279
Query: 654 NALFLETFGPGSETSSATIEWVMAELIKNPDKMAKVREELDTVVGNSTVKESHLPQLPYL 475
NAL +E FG G+ET+++TIEW + EL N ++K+R EL VVG+ TVKES++P LPYL
Sbjct: 280 NALLMELFGAGTETTASTIEWAITELTNNLRVISKLRAELINVVGHKTVKESNIPHLPYL 339
Query: 474 HACIKEAMRLHPAAPFLLPRRAIESCEIMGYTIPKDCQLLVNAYAIGRDPKAWKDPTTFR 295
A +KE +RLHP P LLPRRA+E+C++M YTIPK+CQ++VNA+AIGRDPK W DP TF+
Sbjct: 340 QAIVKETLRLHPPTPLLLPRRALETCKVMNYTIPKECQIMVNAWAIGRDPKTWDDPLTFK 399
Query: 294 PERFLESDVDYHGSHYQFIPFGAGRRICIGMPLATRTIPLIVGSLVHTYDWSLPNGRGAD 115
PERF+ S VDY G+ ++ IPFG GRRIC G+PLA++ + LIV +LV +DWS P G +
Sbjct: 400 PERFMNSTVDYKGNDFELIPFGGGRRICPGLPLASQFLSLIVATLVQNFDWSFPQGMTPN 459
Query: 114 ALEMKENLTLSLAIDPSLCIVPNSR 40
+ M E L L DP L IV +R
Sbjct: 460 EVPMDEKFGLPLQKDPPLLIVLKAR 484
Score = 175 bits (444), Expect(2) = e-116
Identities = 87/186 (46%), Positives = 120/186 (64%)
Frame = -3
Query: 1373 KRPNLPPGPHAWPLIGSLPILFSNPDVPLHITLTNMARTHGPLMTLWLGQQSTVVASSPE 1194
+ +LPPGP P++G+L L P H +A +G L TL LG Q+ VVASSP
Sbjct: 26 RNKDLPPGPRPSPIVGNLLQLGEKP----HAEFAKLAEKYGELFTLKLGSQTVVVASSPA 81
Query: 1193 AATEILKIHDRACSGRYIRMSFRLKHHIKYSLVWSDCTDYWKLLRKIVRTEIFSPKMLQA 1014
AA EILK D+ SGRY+ SFR+ H+ S+VWS+C + WKLLRK+ RTE+FSPKM+++
Sbjct: 82 AAAEILKTRDKILSGRYVFQSFRVYEHVLNSIVWSECNENWKLLRKVCRTELFSPKMIES 141
Query: 1013 QSHVREERVGELMDYLRSKQGQTVKIGKFVFGTLLNILGNAVFSKNVFVYSDDDGGKDVX 834
Q+++RE + +++ +LR K+ Q VKI + VF TL+NI GN +FSK+VF D GG
Sbjct: 142 QAYIREAKALDMVRFLRKKENQEVKIVEVVFNTLVNIFGNLIFSKDVFDLEDPTGGSAEL 201
Query: 833 FFRYWK 816
WK
Sbjct: 202 KEHLWK 207