BLASTX nr result
ID: Aconitum23_contig00016910
seq
BLASTX 2.2.25 [Feb-01-2011] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Aconitum23_contig00016910 (695 letters) Database: ./nr 77,306,371 sequences; 28,104,191,420 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|XP_010278923.1| PREDICTED: protein POLLEN DEFECTIVE IN GUIDA... 101 5e-19 ref|XP_010278853.1| PREDICTED: protein POLLEN DEFECTIVE IN GUIDA... 101 5e-19 ref|XP_010271212.1| PREDICTED: protein POLLEN DEFECTIVE IN GUIDA... 96 2e-17 ref|XP_010271211.1| PREDICTED: protein POLLEN DEFECTIVE IN GUIDA... 96 2e-17 ref|XP_002281514.1| PREDICTED: protein POLLEN DEFECTIVE IN GUIDA... 87 1e-14 emb|CBI26801.3| unnamed protein product [Vitis vinifera] 87 1e-14 emb|CAN62475.1| hypothetical protein VITISV_005323 [Vitis vinifera] 87 1e-14 ref|XP_011658997.1| PREDICTED: protein POLLEN DEFECTIVE IN GUIDA... 62 3e-07 gb|KGN44190.1| hypothetical protein Csa_7G219260 [Cucumis sativus] 62 3e-07 ref|XP_004139799.1| PREDICTED: protein POLLEN DEFECTIVE IN GUIDA... 62 3e-07 ref|XP_008447822.1| PREDICTED: protein POLLEN DEFECTIVE IN GUIDA... 62 3e-07 ref|XP_008447821.1| PREDICTED: protein POLLEN DEFECTIVE IN GUIDA... 62 3e-07 ref|XP_008447820.1| PREDICTED: protein POLLEN DEFECTIVE IN GUIDA... 62 3e-07 >ref|XP_010278923.1| PREDICTED: protein POLLEN DEFECTIVE IN GUIDANCE 1-like isoform X2 [Nelumbo nucifera] Length = 620 Score = 101 bits (251), Expect = 5e-19 Identities = 75/214 (35%), Positives = 105/214 (49%), Gaps = 16/214 (7%) Frame = -2 Query: 601 MALRYGGRKLSFDVLSGDSASADDESTLLLRSI---SDSFPTGT-----ETKPSXXXXXX 446 MALR GGRKLSFD+L D + D+ S +DS G +T Sbjct: 1 MALRSGGRKLSFDILRSDGSINDEFPFYRSNSAPGRADSGDVGRVDPAGKTNRRKRKNKG 60 Query: 445 XXXXXKSVIEESLLEEPITEKRVDAVFDDR-----SNTSNNGACELDFQSYSIQTVICEE 281 K I+ + E+ I +K VD+VFDDR N S E D +SY+IQTV+CEE Sbjct: 61 SKKKKKVDIDSPIDEDQIIDKSVDSVFDDRKPSFYENASFENGMESDHRSYTIQTVVCEE 120 Query: 280 VRVPEKENGSVRTVHRVSEKDFEILNGXXXXXXXXXXXXXXXXSGTIDESIVMS---VAK 110 + VPE+ S+RT+H+V E DF+ L+G D+S+ + Sbjct: 121 ITVPEENATSIRTLHQVDEHDFQNLHGESHQPGEFRQRSVNGCC-LCDDSLSPADGPAKV 179 Query: 109 EASIAELSSGGQLKSEVNGNFANNKLEKTESLDW 8 E+++ SS GQ ++ NG+ KLE +ESLDW Sbjct: 180 ESNVGLNSSLGQWSTQPNGSVVTRKLETSESLDW 213 >ref|XP_010278853.1| PREDICTED: protein POLLEN DEFECTIVE IN GUIDANCE 1-like isoform X1 [Nelumbo nucifera] Length = 633 Score = 101 bits (251), Expect = 5e-19 Identities = 75/214 (35%), Positives = 105/214 (49%), Gaps = 16/214 (7%) Frame = -2 Query: 601 MALRYGGRKLSFDVLSGDSASADDESTLLLRSI---SDSFPTGT-----ETKPSXXXXXX 446 MALR GGRKLSFD+L D + D+ S +DS G +T Sbjct: 1 MALRSGGRKLSFDILRSDGSINDEFPFYRSNSAPGRADSGDVGRVDPAGKTNRRKRKNKG 60 Query: 445 XXXXXKSVIEESLLEEPITEKRVDAVFDDR-----SNTSNNGACELDFQSYSIQTVICEE 281 K I+ + E+ I +K VD+VFDDR N S E D +SY+IQTV+CEE Sbjct: 61 SKKKKKVDIDSPIDEDQIIDKSVDSVFDDRKPSFYENASFENGMESDHRSYTIQTVVCEE 120 Query: 280 VRVPEKENGSVRTVHRVSEKDFEILNGXXXXXXXXXXXXXXXXSGTIDESIVMS---VAK 110 + VPE+ S+RT+H+V E DF+ L+G D+S+ + Sbjct: 121 ITVPEENATSIRTLHQVDEHDFQNLHGESHQPGEFRQRSVNGCC-LCDDSLSPADGPAKV 179 Query: 109 EASIAELSSGGQLKSEVNGNFANNKLEKTESLDW 8 E+++ SS GQ ++ NG+ KLE +ESLDW Sbjct: 180 ESNVGLNSSLGQWSTQPNGSVVTRKLETSESLDW 213 >ref|XP_010271212.1| PREDICTED: protein POLLEN DEFECTIVE IN GUIDANCE 1-like isoform X2 [Nelumbo nucifera] Length = 512 Score = 96.3 bits (238), Expect = 2e-17 Identities = 87/218 (39%), Positives = 112/218 (51%), Gaps = 20/218 (9%) Frame = -2 Query: 601 MALRYGGRKLSFDVLSGDSASADDESTLLLRSISDSFPT--GTE-------TKPSXXXXX 449 M LR G RKLSFD+LSG+ S DDE +L RS SD G E KP+ Sbjct: 1 MVLRSGRRKLSFDILSGNG-SMDDEF-VLFRSNSDQVRQIDGDEGRVDPGGDKPNRRKRK 58 Query: 448 XXXXXXKSV-IEESLLEEPITEKR-VDAVF-DDRSNTSNNGACE----LDFQSYSIQTVI 290 K I+ S+ E+P+T VD+V D RS NG+C+ D+QSY QTVI Sbjct: 59 NKSSKKKKYYIDCSIEEDPVTGNTGVDSVLVDQRSCLYENGSCDNGVKSDYQSYRSQTVI 118 Query: 289 CEEVRVPEKENGSVRTVHRVSEKDFEILNGXXXXXXXXXXXXXXXXSGTIDESIVMS--V 116 CEEV V E+ + +VRTVH V+E DF+ L+G SG +ES+ + Sbjct: 119 CEEVTVSEENDANVRTVHHVAEHDFQNLHG-DRHQSGELRQRSVGGSGLCNESVSRTGEP 177 Query: 115 AKEASIAEL--SSGGQLKSEVNGNFANNKLEKTESLDW 8 AKE + L SS GQ + + NG+ KLE SLDW Sbjct: 178 AKEENSVGLKSSSSGQWRPDPNGSAVTTKLETELSLDW 215 >ref|XP_010271211.1| PREDICTED: protein POLLEN DEFECTIVE IN GUIDANCE 1-like isoform X1 [Nelumbo nucifera] Length = 635 Score = 96.3 bits (238), Expect = 2e-17 Identities = 87/218 (39%), Positives = 112/218 (51%), Gaps = 20/218 (9%) Frame = -2 Query: 601 MALRYGGRKLSFDVLSGDSASADDESTLLLRSISDSFPT--GTE-------TKPSXXXXX 449 M LR G RKLSFD+LSG+ S DDE +L RS SD G E KP+ Sbjct: 1 MVLRSGRRKLSFDILSGNG-SMDDEF-VLFRSNSDQVRQIDGDEGRVDPGGDKPNRRKRK 58 Query: 448 XXXXXXKSV-IEESLLEEPITEKR-VDAVF-DDRSNTSNNGACE----LDFQSYSIQTVI 290 K I+ S+ E+P+T VD+V D RS NG+C+ D+QSY QTVI Sbjct: 59 NKSSKKKKYYIDCSIEEDPVTGNTGVDSVLVDQRSCLYENGSCDNGVKSDYQSYRSQTVI 118 Query: 289 CEEVRVPEKENGSVRTVHRVSEKDFEILNGXXXXXXXXXXXXXXXXSGTIDESIVMS--V 116 CEEV V E+ + +VRTVH V+E DF+ L+G SG +ES+ + Sbjct: 119 CEEVTVSEENDANVRTVHHVAEHDFQNLHG-DRHQSGELRQRSVGGSGLCNESVSRTGEP 177 Query: 115 AKEASIAEL--SSGGQLKSEVNGNFANNKLEKTESLDW 8 AKE + L SS GQ + + NG+ KLE SLDW Sbjct: 178 AKEENSVGLKSSSSGQWRPDPNGSAVTTKLETELSLDW 215 >ref|XP_002281514.1| PREDICTED: protein POLLEN DEFECTIVE IN GUIDANCE 1 [Vitis vinifera] Length = 622 Score = 86.7 bits (213), Expect = 1e-14 Identities = 74/212 (34%), Positives = 106/212 (50%), Gaps = 14/212 (6%) Frame = -2 Query: 601 MALRYGGRKLSFDVLSGDSASADDESTLLLRSISDSFPTGTETKPS------XXXXXXXX 440 M LR GGRKLSF++LS S S +DE TL RS SD PS Sbjct: 1 MDLRRGGRKLSFEILSA-SNSIEDEETLSYRSNSDPIHEDAGVSPSESRTNRRKRKNKGS 59 Query: 439 XXXKSVIEESLLEEPITEKRVDAVFDDRSN-TSNNGAC----ELDFQSYSIQTVICEEVR 275 K I + E+P+T+K +D+VFDD + NG+C ++++Q+YS+Q+V V Sbjct: 60 KKKKKTITCPIDEDPVTDKGIDSVFDDPARVVFENGSCPNGFDVNYQNYSMQSV----VT 115 Query: 274 VPEKENGSVRTVHRVSEKDFEILNGXXXXXXXXXXXXXXXXSG---TIDESIVMSVAKEA 104 V E+ SVRTV +V E +F+ L G G + ++VA+E+ Sbjct: 116 VLEE---SVRTVLQVPESEFQNLRGDGHLLAELRQRSVNGSGGGEEVAGSQVDVNVAEES 172 Query: 103 SIAELSSGGQLKSEVNGNFANNKLEKTESLDW 8 I E+SS G+ + E NG +L+ ESLDW Sbjct: 173 GI-EVSSSGKQRGEPNGGIV-KQLDSAESLDW 202 >emb|CBI26801.3| unnamed protein product [Vitis vinifera] Length = 626 Score = 86.7 bits (213), Expect = 1e-14 Identities = 74/212 (34%), Positives = 106/212 (50%), Gaps = 14/212 (6%) Frame = -2 Query: 601 MALRYGGRKLSFDVLSGDSASADDESTLLLRSISDSFPTGTETKPS------XXXXXXXX 440 M LR GGRKLSF++LS S S +DE TL RS SD PS Sbjct: 1 MDLRRGGRKLSFEILSA-SNSIEDEETLSYRSNSDPIHEDAGVSPSESRTNRRKRKNKGS 59 Query: 439 XXXKSVIEESLLEEPITEKRVDAVFDDRSN-TSNNGAC----ELDFQSYSIQTVICEEVR 275 K I + E+P+T+K +D+VFDD + NG+C ++++Q+YS+Q+V V Sbjct: 60 KKKKKTITCPIDEDPVTDKGIDSVFDDPARVVFENGSCPNGFDVNYQNYSMQSV----VT 115 Query: 274 VPEKENGSVRTVHRVSEKDFEILNGXXXXXXXXXXXXXXXXSG---TIDESIVMSVAKEA 104 V E+ SVRTV +V E +F+ L G G + ++VA+E+ Sbjct: 116 VLEE---SVRTVLQVPESEFQNLRGDGHLLAELRQRSVNGSGGGEEVAGSQVDVNVAEES 172 Query: 103 SIAELSSGGQLKSEVNGNFANNKLEKTESLDW 8 I E+SS G+ + E NG +L+ ESLDW Sbjct: 173 GI-EVSSSGKQRGEPNGGIV-KQLDSAESLDW 202 >emb|CAN62475.1| hypothetical protein VITISV_005323 [Vitis vinifera] Length = 712 Score = 86.7 bits (213), Expect = 1e-14 Identities = 75/212 (35%), Positives = 107/212 (50%), Gaps = 14/212 (6%) Frame = -2 Query: 601 MALRYGGRKLSFDVLSGDSASADDESTLLLRSISDSFPTGTETKPS------XXXXXXXX 440 M LR GGRKLSF++LS S S +DE TL RS SD PS Sbjct: 1 MDLRRGGRKLSFEILSA-SNSIEDEETLSYRSNSDPIHEDAGVSPSESXTNRRKRKNKGS 59 Query: 439 XXXKSVIEESLLEEPITEKRVDAVFDDRSN-TSNNGAC----ELDFQSYSIQTVICEEVR 275 K I + E+P+T+K +D+VFDD + NG+C ++++Q+YS+Q+V V Sbjct: 60 KKKKKTITCPIDEDPVTDKGIDSVFDDPARVVFENGSCPNGFDVNYQNYSMQSV----VT 115 Query: 274 VPEKENGSVRTVHRVSEKDFEILNGXXXXXXXXXXXXXXXXSG---TIDESIVMSVAKEA 104 V E+ SVRTV +V E +F+ L G G + ++VA+E+ Sbjct: 116 VLEE---SVRTVLQVPESEFQNLRGDGXLVAELRQRSVNGSGGGEEVAGSQVDVNVAEES 172 Query: 103 SIAELSSGGQLKSEVNGNFANNKLEKTESLDW 8 I E+SS G+ + E NG +L+ ESLDW Sbjct: 173 GI-EVSSSGKQRGEPNGGIV-KQLDSAESLDW 202 >ref|XP_011658997.1| PREDICTED: protein POLLEN DEFECTIVE IN GUIDANCE 1 isoform X2 [Cucumis sativus] Length = 636 Score = 62.4 bits (150), Expect = 3e-07 Identities = 68/226 (30%), Positives = 96/226 (42%), Gaps = 28/226 (12%) Frame = -2 Query: 601 MALRYGGRKLSFDVLSGDSASADDESTLLLRSISDSFPTGTE--------TKPSXXXXXX 446 M LR GGRKLSFDVL G +S +D S L+L S SD G E KP+ Sbjct: 1 MELRSGGRKLSFDVLRGSGSSEEDRS-LILGSNSDPVSNGIEDSGAQHSIEKPNRRKRRH 59 Query: 445 XXXXXKSVIEE-------SLLEEPITEKRV--DAVFD-----DRSNTSNNGAC------E 326 S+ E+PI EK + ++V D R + + +G C E Sbjct: 60 RGSKKNKAAATTTAPSNCSIPEDPIAEKCMISNSVVDKPEDLGRHSVNRDGTCTNRLEFE 119 Query: 325 LDFQSYSIQTVICEEVRVPEKENGSVRTVHRVSEKDFEILNGXXXXXXXXXXXXXXXXSG 146 L+++S S TV +E+ VP++ GS+ + + SE D + L +G Sbjct: 120 LNYRSCSTGTVFYQELTVPDESRGSISILTQGSEVDCQNLRN---DRFSFGELRQRTVNG 176 Query: 145 TIDESIVMSVAKEASIAELSSGGQLKSEVNGNFANNKLEKTESLDW 8 S + E +S + KSE NGN +LE SLDW Sbjct: 177 DDASSRFGDDKNVETCVEANSVVKQKSEPNGNVV-PRLETAGSLDW 221 >gb|KGN44190.1| hypothetical protein Csa_7G219260 [Cucumis sativus] Length = 627 Score = 62.4 bits (150), Expect = 3e-07 Identities = 68/226 (30%), Positives = 96/226 (42%), Gaps = 28/226 (12%) Frame = -2 Query: 601 MALRYGGRKLSFDVLSGDSASADDESTLLLRSISDSFPTGTE--------TKPSXXXXXX 446 M LR GGRKLSFDVL G +S +D S L+L S SD G E KP+ Sbjct: 1 MELRSGGRKLSFDVLRGSGSSEEDRS-LILGSNSDPVSNGIEDSGAQHSIEKPNRRKRRH 59 Query: 445 XXXXXKSVIEE-------SLLEEPITEKRV--DAVFD-----DRSNTSNNGAC------E 326 S+ E+PI EK + ++V D R + + +G C E Sbjct: 60 RGSKKNKAAATTTAPSNCSIPEDPIAEKCMISNSVVDKPEDLGRHSVNRDGTCTNRLEFE 119 Query: 325 LDFQSYSIQTVICEEVRVPEKENGSVRTVHRVSEKDFEILNGXXXXXXXXXXXXXXXXSG 146 L+++S S TV +E+ VP++ GS+ + + SE D + L +G Sbjct: 120 LNYRSCSTGTVFYQELTVPDESRGSISILTQGSEVDCQNLRN---DRFSFGELRQRTVNG 176 Query: 145 TIDESIVMSVAKEASIAELSSGGQLKSEVNGNFANNKLEKTESLDW 8 S + E +S + KSE NGN +LE SLDW Sbjct: 177 DDASSRFGDDKNVETCVEANSVVKQKSEPNGNVV-PRLETAGSLDW 221 >ref|XP_004139799.1| PREDICTED: protein POLLEN DEFECTIVE IN GUIDANCE 1 isoform X1 [Cucumis sativus] Length = 641 Score = 62.4 bits (150), Expect = 3e-07 Identities = 68/226 (30%), Positives = 96/226 (42%), Gaps = 28/226 (12%) Frame = -2 Query: 601 MALRYGGRKLSFDVLSGDSASADDESTLLLRSISDSFPTGTE--------TKPSXXXXXX 446 M LR GGRKLSFDVL G +S +D S L+L S SD G E KP+ Sbjct: 1 MELRSGGRKLSFDVLRGSGSSEEDRS-LILGSNSDPVSNGIEDSGAQHSIEKPNRRKRRH 59 Query: 445 XXXXXKSVIEE-------SLLEEPITEKRV--DAVFD-----DRSNTSNNGAC------E 326 S+ E+PI EK + ++V D R + + +G C E Sbjct: 60 RGSKKNKAAATTTAPSNCSIPEDPIAEKCMISNSVVDKPEDLGRHSVNRDGTCTNRLEFE 119 Query: 325 LDFQSYSIQTVICEEVRVPEKENGSVRTVHRVSEKDFEILNGXXXXXXXXXXXXXXXXSG 146 L+++S S TV +E+ VP++ GS+ + + SE D + L +G Sbjct: 120 LNYRSCSTGTVFYQELTVPDESRGSISILTQGSEVDCQNLRN---DRFSFGELRQRTVNG 176 Query: 145 TIDESIVMSVAKEASIAELSSGGQLKSEVNGNFANNKLEKTESLDW 8 S + E +S + KSE NGN +LE SLDW Sbjct: 177 DDASSRFGDDKNVETCVEANSVVKQKSEPNGNVV-PRLETAGSLDW 221 >ref|XP_008447822.1| PREDICTED: protein POLLEN DEFECTIVE IN GUIDANCE 1 isoform X3 [Cucumis melo] Length = 618 Score = 62.0 bits (149), Expect = 3e-07 Identities = 68/226 (30%), Positives = 96/226 (42%), Gaps = 28/226 (12%) Frame = -2 Query: 601 MALRYGGRKLSFDVLSGDSASADDESTLLLRSISDSFPTGTE--------TKPSXXXXXX 446 M LR GGRKLSFDVL G +S +D S L+L S SD G E KP+ Sbjct: 1 MELRSGGRKLSFDVLRGSGSSEEDRS-LILGSNSDPVLNGVEESGAQHSIEKPNRRKRRH 59 Query: 445 XXXXXKSVIEE-------SLLEEPITEKRV--DAVFD-----DRSNTSNNGACE------ 326 S+ E+PI EK + ++V D R + + +G C Sbjct: 60 RGSKKNKAAATTTAPSNCSIPEDPIAEKCMISNSVVDKPEDLGRLSVNRDGTCTNRLEFG 119 Query: 325 LDFQSYSIQTVICEEVRVPEKENGSVRTVHRVSEKDFEILNGXXXXXXXXXXXXXXXXSG 146 L+++S S TV +E+ VP++ GS+ + + SE D + L +G Sbjct: 120 LNYRSCSTGTVFYQELTVPDESRGSMSILTQGSEVDCQNLRN---DRFSFGELRQRTVNG 176 Query: 145 TIDESIVMSVAKEASIAELSSGGQLKSEVNGNFANNKLEKTESLDW 8 S + E +SG + KSE NGN +LE SLDW Sbjct: 177 DDASSRFGDDRNVENCVEANSGVKQKSEPNGNVV-PRLETAGSLDW 221 >ref|XP_008447821.1| PREDICTED: protein POLLEN DEFECTIVE IN GUIDANCE 1 isoform X2 [Cucumis melo] Length = 636 Score = 62.0 bits (149), Expect = 3e-07 Identities = 68/226 (30%), Positives = 96/226 (42%), Gaps = 28/226 (12%) Frame = -2 Query: 601 MALRYGGRKLSFDVLSGDSASADDESTLLLRSISDSFPTGTE--------TKPSXXXXXX 446 M LR GGRKLSFDVL G +S +D S L+L S SD G E KP+ Sbjct: 1 MELRSGGRKLSFDVLRGSGSSEEDRS-LILGSNSDPVLNGVEESGAQHSIEKPNRRKRRH 59 Query: 445 XXXXXKSVIEE-------SLLEEPITEKRV--DAVFD-----DRSNTSNNGACE------ 326 S+ E+PI EK + ++V D R + + +G C Sbjct: 60 RGSKKNKAAATTTAPSNCSIPEDPIAEKCMISNSVVDKPEDLGRLSVNRDGTCTNRLEFG 119 Query: 325 LDFQSYSIQTVICEEVRVPEKENGSVRTVHRVSEKDFEILNGXXXXXXXXXXXXXXXXSG 146 L+++S S TV +E+ VP++ GS+ + + SE D + L +G Sbjct: 120 LNYRSCSTGTVFYQELTVPDESRGSMSILTQGSEVDCQNLRN---DRFSFGELRQRTVNG 176 Query: 145 TIDESIVMSVAKEASIAELSSGGQLKSEVNGNFANNKLEKTESLDW 8 S + E +SG + KSE NGN +LE SLDW Sbjct: 177 DDASSRFGDDRNVENCVEANSGVKQKSEPNGNVV-PRLETAGSLDW 221 >ref|XP_008447820.1| PREDICTED: protein POLLEN DEFECTIVE IN GUIDANCE 1 isoform X1 [Cucumis melo] Length = 641 Score = 62.0 bits (149), Expect = 3e-07 Identities = 68/226 (30%), Positives = 96/226 (42%), Gaps = 28/226 (12%) Frame = -2 Query: 601 MALRYGGRKLSFDVLSGDSASADDESTLLLRSISDSFPTGTE--------TKPSXXXXXX 446 M LR GGRKLSFDVL G +S +D S L+L S SD G E KP+ Sbjct: 1 MELRSGGRKLSFDVLRGSGSSEEDRS-LILGSNSDPVLNGVEESGAQHSIEKPNRRKRRH 59 Query: 445 XXXXXKSVIEE-------SLLEEPITEKRV--DAVFD-----DRSNTSNNGACE------ 326 S+ E+PI EK + ++V D R + + +G C Sbjct: 60 RGSKKNKAAATTTAPSNCSIPEDPIAEKCMISNSVVDKPEDLGRLSVNRDGTCTNRLEFG 119 Query: 325 LDFQSYSIQTVICEEVRVPEKENGSVRTVHRVSEKDFEILNGXXXXXXXXXXXXXXXXSG 146 L+++S S TV +E+ VP++ GS+ + + SE D + L +G Sbjct: 120 LNYRSCSTGTVFYQELTVPDESRGSMSILTQGSEVDCQNLRN---DRFSFGELRQRTVNG 176 Query: 145 TIDESIVMSVAKEASIAELSSGGQLKSEVNGNFANNKLEKTESLDW 8 S + E +SG + KSE NGN +LE SLDW Sbjct: 177 DDASSRFGDDRNVENCVEANSGVKQKSEPNGNVV-PRLETAGSLDW 221