BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Aconitum23_contig00014237
         (611 letters)

Database: ./nr 
           77,306,371 sequences; 28,104,191,420 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_012078344.1| PREDICTED: probable methyltransferase PMT24 ...    90   8e-16
ref|XP_011006303.1| PREDICTED: probable methyltransferase PMT24 ...    88   4e-15
ref|XP_011006302.1| PREDICTED: probable methyltransferase PMT24 ...    88   4e-15
ref|XP_002307464.2| hypothetical protein POPTR_0005s20670g [Popu...    87   5e-15
ref|XP_002300957.2| hypothetical protein POPTR_0002s07640g [Popu...    84   8e-14
ref|XP_004289881.1| PREDICTED: probable methyltransferase PMT24 ...    83   1e-13
ref|XP_003526869.1| PREDICTED: probable methyltransferase PMT26-...    83   1e-13
gb|KHN46967.1| Putative methyltransferase PMT25 [Glycine soja]         83   1e-13
gb|KHN36073.1| Putative methyltransferase PMT27 [Glycine soja]         83   1e-13
ref|XP_003516560.1| PREDICTED: probable methyltransferase PMT27-...    83   1e-13
gb|KHN41938.1| Putative methyltransferase PMT27 [Glycine soja]         82   2e-13
gb|KHN16949.1| Putative methyltransferase PMT26 [Glycine soja]         82   2e-13
ref|XP_006584650.1| PREDICTED: probable methyltransferase PMT26-...    82   2e-13
ref|XP_003538865.1| PREDICTED: probable methyltransferase PMT27-...    82   2e-13
ref|XP_003527960.1| PREDICTED: probable methyltransferase PMT26-...    82   2e-13
gb|KHN34092.1| Putative methyltransferase PMT26 [Glycine soja]         82   2e-13
ref|XP_007137790.1| hypothetical protein PHAVU_009G155600g [Phas...    82   2e-13
ref|XP_004503920.1| PREDICTED: probable methyltransferase PMT26 ...    82   2e-13
ref|XP_006580338.1| PREDICTED: probable methyltransferase PMT26-...    82   2e-13
ref|XP_009115237.1| PREDICTED: probable methyltransferase PMT24 ...    82   3e-13

>ref|XP_012078344.1| PREDICTED: probable methyltransferase PMT24 [Jatropha curcas]
           gi|643723291|gb|KDP32896.1| hypothetical protein
           JCGZ_12188 [Jatropha curcas]
          Length = 813

 Score = 90.1 bits (222), Expect = 8e-16
 Identities = 48/101 (47%), Positives = 61/101 (60%), Gaps = 20/101 (19%)
 Frame = -3

Query: 243 GGKLLLELN*VLRPGGYFF-------------VVCNSSIPEPRRRCCYMERESSR----- 118
           GGKLLLELN VLRPGGYF              V   +++ E  +  C+  +   +     
Sbjct: 488 GGKLLLELNRVLRPGGYFVWSATPVYQKLPEDVGIWNAMTELTKSMCWDLKVIKKDKLNG 547

Query: 117 --CSVFRKPTSNECYGQREENEPSLCKDSDDPNTAWNVPLE 1
              ++FRKPTSNECY +R +NEP LCK+SDDPN AWNVPL+
Sbjct: 548 VGAAIFRKPTSNECYNERSQNEPPLCKESDDPNAAWNVPLD 588


>ref|XP_011006303.1| PREDICTED: probable methyltransferase PMT24 isoform X2 [Populus
           euphratica]
          Length = 812

 Score = 87.8 bits (216), Expect = 4e-15
 Identities = 50/101 (49%), Positives = 58/101 (57%), Gaps = 20/101 (19%)
 Frame = -3

Query: 243 GGKLLLELN*VLRPGGYFFVVCN---SSIPEPRRRCCYMER-ESSRC------------- 115
           GGKLLLELN VLRPGGYF          +PE       M +   S C             
Sbjct: 487 GGKLLLELNRVLRPGGYFVWSATPVYQKLPEDVGIWKAMSKLTKSMCWDLVVIKKDKLNG 546

Query: 114 ---SVFRKPTSNECYGQREENEPSLCKDSDDPNTAWNVPLE 1
              ++FRKPTSN+CY +R +NEP LCK+SDDPN AWNVPLE
Sbjct: 547 VGAAIFRKPTSNDCYNKRPQNEPPLCKESDDPNAAWNVPLE 587


>ref|XP_011006302.1| PREDICTED: probable methyltransferase PMT24 isoform X1 [Populus
           euphratica]
          Length = 826

 Score = 87.8 bits (216), Expect = 4e-15
 Identities = 50/101 (49%), Positives = 58/101 (57%), Gaps = 20/101 (19%)
 Frame = -3

Query: 243 GGKLLLELN*VLRPGGYFFVVCN---SSIPEPRRRCCYMER-ESSRC------------- 115
           GGKLLLELN VLRPGGYF          +PE       M +   S C             
Sbjct: 501 GGKLLLELNRVLRPGGYFVWSATPVYQKLPEDVGIWKAMSKLTKSMCWDLVVIKKDKLNG 560

Query: 114 ---SVFRKPTSNECYGQREENEPSLCKDSDDPNTAWNVPLE 1
              ++FRKPTSN+CY +R +NEP LCK+SDDPN AWNVPLE
Sbjct: 561 VGAAIFRKPTSNDCYNKRPQNEPPLCKESDDPNAAWNVPLE 601


>ref|XP_002307464.2| hypothetical protein POPTR_0005s20670g [Populus trichocarpa]
           gi|550339404|gb|EEE94460.2| hypothetical protein
           POPTR_0005s20670g [Populus trichocarpa]
          Length = 826

 Score = 87.4 bits (215), Expect = 5e-15
 Identities = 50/101 (49%), Positives = 57/101 (56%), Gaps = 20/101 (19%)
 Frame = -3

Query: 243 GGKLLLELN*VLRPGGYFFVVCN---SSIPEPRRRCCYMER-ESSRC------------- 115
           GGKLLLELN VLRPGGYF          +PE       M +   S C             
Sbjct: 501 GGKLLLELNRVLRPGGYFVWSATPVYQKLPEDVGIWKAMSKLTKSMCWDLVVIKKDKLNG 560

Query: 114 ---SVFRKPTSNECYGQREENEPSLCKDSDDPNTAWNVPLE 1
              ++FRKPTSN+CY  R +NEP LCK+SDDPN AWNVPLE
Sbjct: 561 VGAAIFRKPTSNDCYNNRPQNEPPLCKESDDPNAAWNVPLE 601


>ref|XP_002300957.2| hypothetical protein POPTR_0002s07640g [Populus trichocarpa]
           gi|118485999|gb|ABK94843.1| unknown [Populus
           trichocarpa] gi|550344490|gb|EEE80230.2| hypothetical
           protein POPTR_0002s07640g [Populus trichocarpa]
          Length = 817

 Score = 83.6 bits (205), Expect = 8e-14
 Identities = 49/104 (47%), Positives = 58/104 (55%), Gaps = 23/104 (22%)
 Frame = -3

Query: 243 GGKLLLELN*VLRPGGYFFVVCNSSIPEPRRRC-------CYMERESSRC---------- 115
           GGKLLLELN VLRPGGYF     S+ P  R+R           +   S C          
Sbjct: 490 GGKLLLELNRVLRPGGYFVW---SATPVYRKRPEDVGIWKAMSKLTKSMCWDLVVIKTDT 546

Query: 114 ------SVFRKPTSNECYGQREENEPSLCKDSDDPNTAWNVPLE 1
                 +++RKPTSN+CY  R +NEP LCK+SDDPN AWNV LE
Sbjct: 547 LNGVGAAIYRKPTSNDCYNNRPQNEPPLCKESDDPNAAWNVLLE 590


>ref|XP_004289881.1| PREDICTED: probable methyltransferase PMT24 [Fragaria vesca subsp.
           vesca]
          Length = 797

 Score = 83.2 bits (204), Expect = 1e-13
 Identities = 47/101 (46%), Positives = 57/101 (56%), Gaps = 20/101 (19%)
 Frame = -3

Query: 243 GGKLLLELN*VLRPGGYFFVVCN---SSIPEPRRRCCYMER-ESSRC------------- 115
           GGKLLLELN VLRPGGYF          +PE       M +   S C             
Sbjct: 471 GGKLLLELNRVLRPGGYFVWSATPVYQKLPEDVGIWKAMSKLTKSMCWDLVLVRNDKLNQ 530

Query: 114 ---SVFRKPTSNECYGQREENEPSLCKDSDDPNTAWNVPLE 1
              +++RKP++NECY +R ENEP+LC +SDDPN AW VPLE
Sbjct: 531 VAAAIYRKPSTNECYNERAENEPALCSESDDPNAAWKVPLE 571


>ref|XP_003526869.1| PREDICTED: probable methyltransferase PMT26-like isoform X1
           [Glycine max] gi|571460696|ref|XP_006581774.1|
           PREDICTED: probable methyltransferase PMT26-like isoform
           X2 [Glycine max] gi|947105532|gb|KRH53915.1|
           hypothetical protein GLYMA_06G154800 [Glycine max]
           gi|947105533|gb|KRH53916.1| hypothetical protein
           GLYMA_06G154800 [Glycine max]
          Length = 806

 Score = 83.2 bits (204), Expect = 1e-13
 Identities = 47/101 (46%), Positives = 57/101 (56%), Gaps = 20/101 (19%)
 Frame = -3

Query: 243 GGKLLLELN*VLRPGGYFFVVCN---SSIPEP----------RRRCCYMERESSR----- 118
           GGKLLLELN VLRPGG+F          +PE            +  C+     S+     
Sbjct: 480 GGKLLLELNRVLRPGGFFVWSATPIYQKLPEDVEIWKAMKALTKAMCWEVVSISKDPVNG 539

Query: 117 --CSVFRKPTSNECYGQREENEPSLCKDSDDPNTAWNVPLE 1
              +V+RKPTSNECY QR +NEP LC DSDDPN AWN+ L+
Sbjct: 540 VGVAVYRKPTSNECYEQRSKNEPPLCPDSDDPNAAWNIQLQ 580


>gb|KHN46967.1| Putative methyltransferase PMT25 [Glycine soja]
          Length = 689

 Score = 82.8 bits (203), Expect = 1e-13
 Identities = 45/101 (44%), Positives = 57/101 (56%), Gaps = 20/101 (19%)
 Frame = -3

Query: 243 GGKLLLELN*VLRPGGYFF-------------VVCNSSIPEPRRRCCYM-------ERES 124
           GGKLLLELN VLRPGGYF              V    ++ E  +  C+        +   
Sbjct: 362 GGKLLLELNRVLRPGGYFVWSATPVYQKDPEDVEIWKAMDEITKSMCWDLVVIAKDKLNG 421

Query: 123 SRCSVFRKPTSNECYGQREENEPSLCKDSDDPNTAWNVPLE 1
              +++RKPT NECY  R +NEPS+C +SDDPNTAWNV L+
Sbjct: 422 VAAAIYRKPTDNECYNNRIKNEPSMCSESDDPNTAWNVSLQ 462


>gb|KHN36073.1| Putative methyltransferase PMT27 [Glycine soja]
          Length = 796

 Score = 82.8 bits (203), Expect = 1e-13
 Identities = 47/101 (46%), Positives = 56/101 (55%), Gaps = 20/101 (19%)
 Frame = -3

Query: 243 GGKLLLELN*VLRPGGYFF-------------VVCNSSIPEPRRRCCYMERESSR----- 118
           GG LLLELN VLRPGGYF              V     +    +  C+     ++     
Sbjct: 480 GGMLLLELNRVLRPGGYFVWSATPVYQKLEEDVEIWKEMTSLTKSICWELVTINKDGLNK 539

Query: 117 --CSVFRKPTSNECYGQREENEPSLCKDSDDPNTAWNVPLE 1
              +V+RKPTSNECY QRE+NEP LCKD DDPN AW VPL+
Sbjct: 540 VGAAVYRKPTSNECYEQREKNEPPLCKDDDDPNAAWYVPLQ 580


>ref|XP_003516560.1| PREDICTED: probable methyltransferase PMT27-like [Glycine max]
           gi|947128876|gb|KRH76730.1| hypothetical protein
           GLYMA_01G170800 [Glycine max]
          Length = 796

 Score = 82.8 bits (203), Expect = 1e-13
 Identities = 47/101 (46%), Positives = 56/101 (55%), Gaps = 20/101 (19%)
 Frame = -3

Query: 243 GGKLLLELN*VLRPGGYFF-------------VVCNSSIPEPRRRCCYMERESSR----- 118
           GG LLLELN VLRPGGYF              V     +    +  C+     ++     
Sbjct: 480 GGMLLLELNRVLRPGGYFVWSATPVYQKLEEDVEIWKEMTSLTKSICWELVTINKDGLNK 539

Query: 117 --CSVFRKPTSNECYGQREENEPSLCKDSDDPNTAWNVPLE 1
              +V+RKPTSNECY QRE+NEP LCKD DDPN AW VPL+
Sbjct: 540 VGAAVYRKPTSNECYEQREKNEPPLCKDDDDPNAAWYVPLQ 580


>gb|KHN41938.1| Putative methyltransferase PMT27 [Glycine soja]
          Length = 768

 Score = 82.4 bits (202), Expect = 2e-13
 Identities = 47/100 (47%), Positives = 57/100 (57%), Gaps = 20/100 (20%)
 Frame = -3

Query: 243 GGKLLLELN*VLRPGGYFF-------------VVCNSSIPEPRRRCCY----MERESSR- 118
           GG LLLELN VLRPGGYF              V     +    +  C+    ++++    
Sbjct: 451 GGMLLLELNRVLRPGGYFVWSATPVYQKLEEDVEIWKEMTSLTKSICWELVTIKKDGLNK 510

Query: 117 --CSVFRKPTSNECYGQREENEPSLCKDSDDPNTAWNVPL 4
              +V+RKPTSNECY QRE+NEP LCKD DDPN AW VPL
Sbjct: 511 VGAAVYRKPTSNECYEQREKNEPPLCKDEDDPNAAWYVPL 550


>gb|KHN16949.1| Putative methyltransferase PMT26 [Glycine soja]
          Length = 832

 Score = 82.4 bits (202), Expect = 2e-13
 Identities = 45/101 (44%), Positives = 58/101 (57%), Gaps = 20/101 (19%)
 Frame = -3

Query: 243 GGKLLLELN*VLRPGGYFFVVCN---SSIPEP----------RRRCCYMERESSR----- 118
           GGKLLLELN VLRPGG+F          +PE            +  C+     S+     
Sbjct: 506 GGKLLLELNRVLRPGGFFVWSATPIYQKLPEDVEIWNEMKALTKAMCWEVVSISKDKLNG 565

Query: 117 --CSVFRKPTSNECYGQREENEPSLCKDSDDPNTAWNVPLE 1
              +V++KPTSNECY +R +N+P +C DSDDPN AWNVPL+
Sbjct: 566 VGIAVYKKPTSNECYEKRSQNQPPICPDSDDPNAAWNVPLQ 606


>ref|XP_006584650.1| PREDICTED: probable methyltransferase PMT26-like isoform X1
           [Glycine max] gi|571469223|ref|XP_006584651.1|
           PREDICTED: probable methyltransferase PMT26-like isoform
           X2 [Glycine max] gi|571469225|ref|XP_006584652.1|
           PREDICTED: probable methyltransferase PMT26-like isoform
           X3 [Glycine max] gi|947092358|gb|KRH40943.1|
           hypothetical protein GLYMA_08G001200 [Glycine max]
           gi|947092359|gb|KRH40944.1| hypothetical protein
           GLYMA_08G001200 [Glycine max]
          Length = 842

 Score = 82.4 bits (202), Expect = 2e-13
 Identities = 45/101 (44%), Positives = 58/101 (57%), Gaps = 20/101 (19%)
 Frame = -3

Query: 243 GGKLLLELN*VLRPGGYFFVVCN---SSIPEP----------RRRCCYMERESSR----- 118
           GGKLLLELN VLRPGG+F          +PE            +  C+     S+     
Sbjct: 516 GGKLLLELNRVLRPGGFFVWSATPIYQKLPEDVEIWNEMKALTKAMCWEVVSISKDKLNG 575

Query: 117 --CSVFRKPTSNECYGQREENEPSLCKDSDDPNTAWNVPLE 1
              +V++KPTSNECY +R +N+P +C DSDDPN AWNVPL+
Sbjct: 576 VGIAVYKKPTSNECYEKRSQNQPPICPDSDDPNAAWNVPLQ 616


>ref|XP_003538865.1| PREDICTED: probable methyltransferase PMT27-like [Glycine max]
           gi|947079943|gb|KRH28732.1| hypothetical protein
           GLYMA_11G072300 [Glycine max]
          Length = 768

 Score = 82.4 bits (202), Expect = 2e-13
 Identities = 47/100 (47%), Positives = 57/100 (57%), Gaps = 20/100 (20%)
 Frame = -3

Query: 243 GGKLLLELN*VLRPGGYFF-------------VVCNSSIPEPRRRCCY----MERESSR- 118
           GG LLLELN VLRPGGYF              V     +    +  C+    ++++    
Sbjct: 451 GGMLLLELNRVLRPGGYFVWSATPVYQKLEEDVEIWKEMTSLTKSICWELVTIKKDGLNK 510

Query: 117 --CSVFRKPTSNECYGQREENEPSLCKDSDDPNTAWNVPL 4
              +V+RKPTSNECY QRE+NEP LCKD DDPN AW VPL
Sbjct: 511 VGAAVYRKPTSNECYEQREKNEPPLCKDEDDPNAAWYVPL 550


>ref|XP_003527960.1| PREDICTED: probable methyltransferase PMT26-like [Glycine max]
           gi|947104947|gb|KRH53330.1| hypothetical protein
           GLYMA_06G119400 [Glycine max]
           gi|947104948|gb|KRH53331.1| hypothetical protein
           GLYMA_06G119400 [Glycine max]
          Length = 835

 Score = 82.4 bits (202), Expect = 2e-13
 Identities = 45/101 (44%), Positives = 57/101 (56%), Gaps = 20/101 (19%)
 Frame = -3

Query: 243 GGKLLLELN*VLRPGGYFF-------------VVCNSSIPEPRRRCCYM-------ERES 124
           GGKLLLELN VLRPGGYF              V    ++ E  +  C+        +   
Sbjct: 508 GGKLLLELNRVLRPGGYFVWSATPVYQKDPEDVEIWKAMGEITKSMCWDLVVIAKDKLNG 567

Query: 123 SRCSVFRKPTSNECYGQREENEPSLCKDSDDPNTAWNVPLE 1
              +++RKPT NECY  R +NEPS+C +SDDPNTAWNV L+
Sbjct: 568 VAAAIYRKPTDNECYNNRIKNEPSMCSESDDPNTAWNVSLQ 608


>gb|KHN34092.1| Putative methyltransferase PMT26 [Glycine soja]
          Length = 849

 Score = 82.0 bits (201), Expect = 2e-13
 Identities = 44/101 (43%), Positives = 58/101 (57%), Gaps = 20/101 (19%)
 Frame = -3

Query: 243 GGKLLLELN*VLRPGGYFFVVCN---SSIPEP----------RRRCCYMERESSR----- 118
           GGKLLLELN VLRPGG+F          +PE            +  C+     S+     
Sbjct: 523 GGKLLLELNRVLRPGGFFVWSATPIYQKLPEDVEIWNEMKALTKAMCWEVVSISKDKLNG 582

Query: 117 --CSVFRKPTSNECYGQREENEPSLCKDSDDPNTAWNVPLE 1
              +V++KPTSNECY +R +N+P +C DSDDPN AWN+PL+
Sbjct: 583 VGIAVYKKPTSNECYEKRSQNQPPICPDSDDPNAAWNIPLQ 623


>ref|XP_007137790.1| hypothetical protein PHAVU_009G155600g [Phaseolus vulgaris]
           gi|561010877|gb|ESW09784.1| hypothetical protein
           PHAVU_009G155600g [Phaseolus vulgaris]
          Length = 818

 Score = 82.0 bits (201), Expect = 2e-13
 Identities = 47/101 (46%), Positives = 58/101 (57%), Gaps = 20/101 (19%)
 Frame = -3

Query: 243 GGKLLLELN*VLRPGGYFFVVCN---SSIPEP----------RRRCCYMERESSR----- 118
           GGKLLLELN VLRPGG+F          +PE            +  C+     S+     
Sbjct: 492 GGKLLLELNRVLRPGGFFVWSATPIYQKLPEDVEIWNAMKSLTKAICWELVSISKDQVNG 551

Query: 117 --CSVFRKPTSNECYGQREENEPSLCKDSDDPNTAWNVPLE 1
              +V+RKP+SNECY QR +NEP LC+DSDDPN AWNV L+
Sbjct: 552 VGVAVYRKPSSNECYEQRSKNEPPLCQDSDDPNAAWNVKLK 592


>ref|XP_004503920.1| PREDICTED: probable methyltransferase PMT26 [Cicer arietinum]
           gi|502139821|ref|XP_004503921.1| PREDICTED: probable
           methyltransferase PMT26 [Cicer arietinum]
          Length = 819

 Score = 82.0 bits (201), Expect = 2e-13
 Identities = 45/101 (44%), Positives = 57/101 (56%), Gaps = 20/101 (19%)
 Frame = -3

Query: 243 GGKLLLELN*VLRPGGYFFVVCN---SSIPEP----------RRRCCYMERESSR----- 118
           GGKLLLELN VLRPGG F          +PE            +  C+     ++     
Sbjct: 493 GGKLLLELNRVLRPGGLFVWSATPIYQKLPEDVEIWNAMKALTKALCWEVVSITKDKLNG 552

Query: 117 --CSVFRKPTSNECYGQREENEPSLCKDSDDPNTAWNVPLE 1
              +V++KP SNECY QR EN+PS+C DSDDPN AWN+PL+
Sbjct: 553 VGIAVYKKPASNECYEQRSENQPSICPDSDDPNAAWNIPLQ 593


>ref|XP_006580338.1| PREDICTED: probable methyltransferase PMT26-like isoform X1
           [Glycine max] gi|571456274|ref|XP_006580339.1|
           PREDICTED: probable methyltransferase PMT26-like isoform
           X2 [Glycine max] gi|947111265|gb|KRH59591.1|
           hypothetical protein GLYMA_05G193200 [Glycine max]
           gi|947111266|gb|KRH59592.1| hypothetical protein
           GLYMA_05G193200 [Glycine max]
           gi|947111267|gb|KRH59593.1| hypothetical protein
           GLYMA_05G193200 [Glycine max]
          Length = 831

 Score = 82.0 bits (201), Expect = 2e-13
 Identities = 44/101 (43%), Positives = 58/101 (57%), Gaps = 20/101 (19%)
 Frame = -3

Query: 243 GGKLLLELN*VLRPGGYFFVVCN---SSIPEP----------RRRCCYMERESSR----- 118
           GGKLLLELN VLRPGG+F          +PE            +  C+     S+     
Sbjct: 505 GGKLLLELNRVLRPGGFFVWSATPIYQKLPEDVEIWNEMKALTKAMCWEVVSISKDKLNG 564

Query: 117 --CSVFRKPTSNECYGQREENEPSLCKDSDDPNTAWNVPLE 1
              +V++KPTSNECY +R +N+P +C DSDDPN AWN+PL+
Sbjct: 565 VGIAVYKKPTSNECYEKRSQNQPPICPDSDDPNAAWNIPLQ 605


>ref|XP_009115237.1| PREDICTED: probable methyltransferase PMT24 isoform X2 [Brassica
           rapa]
          Length = 725

 Score = 81.6 bits (200), Expect = 3e-13
 Identities = 45/101 (44%), Positives = 56/101 (55%), Gaps = 20/101 (19%)
 Frame = -3

Query: 243 GGKLLLELN*VLRPGGYFF-------------VVCNSSIPEPRRRCCYM-------ERES 124
           GGKLLLELN  LRPGG+F              V    ++ +  +  C+        E   
Sbjct: 398 GGKLLLELNRALRPGGFFVWSATPVYRKTEEDVGIWKAMSKLTKAMCWELKTIKKDELNE 457

Query: 123 SRCSVFRKPTSNECYGQREENEPSLCKDSDDPNTAWNVPLE 1
              +V++KP +NECY QR +NEP LCKDSDD N AWNVPLE
Sbjct: 458 VSAAVYQKPMTNECYNQRSQNEPPLCKDSDDQNAAWNVPLE 498