BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Aconitum23_contig00011667
         (1327 letters)

Database: ./nr 
           77,306,371 sequences; 28,104,191,420 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002282827.2| PREDICTED: mitochondrial tRNA-specific 2-thi...   604   e-170
ref|XP_008449720.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   601   e-169
ref|XP_004142116.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   600   e-169
ref|XP_010257320.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   597   e-167
ref|XP_010102337.1| tRNA-specific 2-thiouridylase mnmA [Morus no...   595   e-167
gb|KDO61266.1| hypothetical protein CISIN_1g042717mg, partial [C...   593   e-166
ref|XP_006470603.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   593   e-166
ref|XP_008238179.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   591   e-166
ref|XP_012446852.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   583   e-163
ref|XP_007209892.1| hypothetical protein PRUPE_ppa004048mg [Prun...   583   e-163
gb|KHG00493.1| tRNA-specific 2-thiouridylase mnmA [Gossypium arb...   582   e-163
ref|XP_002513510.1| tRNA (5-methylaminomethyl-2-thiouridylate)-m...   582   e-163
ref|XP_002298404.2| hypothetical protein POPTR_0001s26620g [Popu...   574   e-161
ref|XP_011006202.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   573   e-160
ref|XP_004507093.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   572   e-160
ref|XP_010045014.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   571   e-160
gb|KCW87153.1| hypothetical protein EUGRSUZ_B03681 [Eucalyptus g...   571   e-160
ref|XP_011045440.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   570   e-159
ref|XP_010326622.1| PREDICTED: mitochondrial tRNA-specific 2-thi...   569   e-159
gb|KNA16767.1| hypothetical protein SOVF_086320 [Spinacia oleracea]   569   e-159

>ref|XP_002282827.2| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 [Vitis
            vinifera] gi|297746327|emb|CBI16383.3| unnamed protein
            product [Vitis vinifera]
          Length = 544

 Score =  604 bits (1557), Expect = e-170
 Identities = 291/399 (72%), Positives = 331/399 (82%)
 Frame = -3

Query: 1325 QIGVPLEVVHLTDEYWSNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYV 1146
            Q+ VPLEVVHLTDEYW NVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAIS+ EFDYV
Sbjct: 149  QVDVPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISSKEFDYV 208

Query: 1145 ASGHYARLVHSSSDEDNERGVSMLELSNDMVKDQTYFLSHLSQNQIKRLLFPLGCISKDE 966
            ASGHYA +VHS +D+ ++   S+LELS DMVKDQTYFLSHLSQ Q+KRL+FPLGCI KDE
Sbjct: 209  ASGHYANVVHSFTDQMDKP--SVLELSKDMVKDQTYFLSHLSQIQLKRLIFPLGCIPKDE 266

Query: 965  VRRLATKFDLPNKERKDSQGICFLGKIKFSEFVARHIGELEGVLLEAETGDFLGKHRGFW 786
            VR LATK DLPN++RKDSQGICFLGKIKFSEFVARHIGE EG++LEAETGD LGKHRGFW
Sbjct: 267  VRSLATKLDLPNRDRKDSQGICFLGKIKFSEFVARHIGEKEGIILEAETGDLLGKHRGFW 326

Query: 785  FYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYFSLDKKRRRFRVGSLKWLSGSPPDN 606
            FYTIGQRQGLRLPGGPWYVVEKD KNNVVFVSRNYFSLDK+RR FRVGSLKW+S  PP  
Sbjct: 327  FYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSLDKRRRLFRVGSLKWISREPPGQ 386

Query: 605  FDEMQCKVRHGPNLYRCTLTMEESVEGKGDAGLVQLFEDDQGLASGQFAAFYKGKTCLGS 426
             +++QCKVRHGP  Y C+ TME   +G  D  +VQL EDDQGLA+GQF AFY+G++C+GS
Sbjct: 387  INQLQCKVRHGPGFYNCSFTMEPGEDGHEDVAVVQLSEDDQGLAAGQFTAFYQGRSCIGS 446

Query: 425  GIILESWEDQGFPVCARALEIARMEDKSKLGKPVKIMFKPEASITNSDHKDNVELPREHV 246
            G+ILESW+DQGFPVCA+ALEIARMEDKSKLGKPVKI  +PE     SD  D ++L RE  
Sbjct: 447  GVILESWDDQGFPVCAKALEIARMEDKSKLGKPVKIKVRPETPPKGSDQIDGIKLDRELA 506

Query: 245  TKQVVASGTTDTQRLASRGVLSSVPLIWLQTLRSKWLRL 129
              Q  A+ T +  R +     S  P+ WLQ +R KWL++
Sbjct: 507  NSQ--AAVTDEISRSSEGEARSRFPMNWLQKIREKWLQM 543


>ref|XP_008449720.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 [Cucumis
            melo]
          Length = 539

 Score =  601 bits (1549), Expect = e-169
 Identities = 287/400 (71%), Positives = 338/400 (84%)
 Frame = -3

Query: 1325 QIGVPLEVVHLTDEYWSNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYV 1146
            QI VPLEVVHLT+EYW NVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYV
Sbjct: 144  QIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYV 203

Query: 1145 ASGHYARLVHSSSDEDNERGVSMLELSNDMVKDQTYFLSHLSQNQIKRLLFPLGCISKDE 966
            ASGHYA ++H S+D+ ++   S+LELS DMVKDQTYFLSHLSQNQ+KRLLFPLGCI KDE
Sbjct: 204  ASGHYANVIHPSADQMDKP--SVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 261

Query: 965  VRRLATKFDLPNKERKDSQGICFLGKIKFSEFVARHIGELEGVLLEAETGDFLGKHRGFW 786
            VR+LA KF+LPNK+RKDSQGICFLGKIKFSEFVARHIGE EGV+LEAE+GDFLGKHRGFW
Sbjct: 262  VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 321

Query: 785  FYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYFSLDKKRRRFRVGSLKWLSGSPPDN 606
            FYT+GQRQGLRLPGGPWYVVEKD KNNVVFVSRNYFS DK+RR FRVGSLKWLSG PPD 
Sbjct: 322  FYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGMPPDR 381

Query: 605  FDEMQCKVRHGPNLYRCTLTMEESVEGKGDAGLVQLFEDDQGLASGQFAAFYKGKTCLGS 426
            F  +QCKVRHGP  Y C LTME + +G  D  +V L EDDQGLA+GQFAAFYKG++C+GS
Sbjct: 382  FTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGS 441

Query: 425  GIILESWEDQGFPVCARALEIARMEDKSKLGKPVKIMFKPEASITNSDHKDNVELPREHV 246
            G+ILESW+DQGFPVC +A+E A+MEDKSKLGKP+KI  KP++ +  S  KD+ ++  +  
Sbjct: 442  GVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKPDSGMNESKAKDDTKI--QDK 499

Query: 245  TKQVVASGTTDTQRLASRGVLSSVPLIWLQTLRSKWLRLF 126
            ++   A+     ++++    +++ P+ WL +LR K L++F
Sbjct: 500  SRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539


>ref|XP_004142116.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 [Cucumis
            sativus] gi|700199017|gb|KGN54175.1| hypothetical protein
            Csa_4G291390 [Cucumis sativus]
          Length = 538

 Score =  600 bits (1548), Expect = e-169
 Identities = 289/400 (72%), Positives = 336/400 (84%)
 Frame = -3

Query: 1325 QIGVPLEVVHLTDEYWSNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYV 1146
            Q+ VPLEVVHLT+EYW NVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYV
Sbjct: 143  QVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYV 202

Query: 1145 ASGHYARLVHSSSDEDNERGVSMLELSNDMVKDQTYFLSHLSQNQIKRLLFPLGCISKDE 966
            ASGHYA ++H S+D+ ++   S+LELS DMVKDQTYFLSHLSQNQ+KRLLFPLGCI KDE
Sbjct: 203  ASGHYANVIHPSADQMDKP--SVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 260

Query: 965  VRRLATKFDLPNKERKDSQGICFLGKIKFSEFVARHIGELEGVLLEAETGDFLGKHRGFW 786
            VR+LA KF+LPNK+RKDSQGICFLGKIKFSEFVARHIGE EGV+LEAE+GDFLGKHRGFW
Sbjct: 261  VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 320

Query: 785  FYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYFSLDKKRRRFRVGSLKWLSGSPPDN 606
            FYTIGQRQGLRLPGGPWYVVEKD KNNVVFVSRNYFS DK+RR FRVGSLKWLSG PPD 
Sbjct: 321  FYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLSGFPPDQ 380

Query: 605  FDEMQCKVRHGPNLYRCTLTMEESVEGKGDAGLVQLFEDDQGLASGQFAAFYKGKTCLGS 426
            F E+QCKVRHGP+ Y C LTME S +G  D  +V L EDDQGLA+GQFAAFYKG++C+GS
Sbjct: 381  FTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSEDDQGLAAGQFAAFYKGRSCIGS 440

Query: 425  GIILESWEDQGFPVCARALEIARMEDKSKLGKPVKIMFKPEASITNSDHKDNVELPREHV 246
            G+ILESW+DQGFPVC +A+E A+MEDKSKLGKP+KI  KPE+ +  S  KD+ E+     
Sbjct: 441  GVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKPESGMKESKAKDDTEI--HDK 498

Query: 245  TKQVVASGTTDTQRLASRGVLSSVPLIWLQTLRSKWLRLF 126
             +   A+     + ++    +++  + WL +L+ K L++F
Sbjct: 499  LRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKKLLQIF 538


>ref|XP_010257320.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like
            [Nelumbo nucifera]
          Length = 531

 Score =  597 bits (1538), Expect = e-167
 Identities = 285/400 (71%), Positives = 336/400 (84%)
 Frame = -3

Query: 1325 QIGVPLEVVHLTDEYWSNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYV 1146
            Q+ VPLEVVHLTDEYW NVVSYII+EYR GRTPNPDVLCNTRIKFGAF+DAISNMEFDYV
Sbjct: 140  QVEVPLEVVHLTDEYWDNVVSYIIQEYRYGRTPNPDVLCNTRIKFGAFLDAISNMEFDYV 199

Query: 1145 ASGHYARLVHSSSDEDNERGVSMLELSNDMVKDQTYFLSHLSQNQIKRLLFPLGCISKDE 966
            ASGHYA ++H  +D+ +  G S+LELS D VKDQTYFLSHLSQ Q+KRLLFPLGCISK+E
Sbjct: 200  ASGHYANVIHPCTDQKD--GPSILELSEDKVKDQTYFLSHLSQAQLKRLLFPLGCISKNE 257

Query: 965  VRRLATKFDLPNKERKDSQGICFLGKIKFSEFVARHIGELEGVLLEAETGDFLGKHRGFW 786
            VR+LA K +LPN++RKDSQGICFLGKIKFSEF+A+HIGE+EG+LLEAETGDFLGKH GFW
Sbjct: 258  VRKLAAKLELPNRDRKDSQGICFLGKIKFSEFIAKHIGEMEGILLEAETGDFLGKHHGFW 317

Query: 785  FYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYFSLDKKRRRFRVGSLKWLSGSPPDN 606
            FYTIGQRQGLRLPGGPWYVVEKD KNNVVFVSRNY+S DK+RRRFRVGSLKWL+G PPD 
Sbjct: 318  FYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYYSFDKRRRRFRVGSLKWLAGLPPDQ 377

Query: 605  FDEMQCKVRHGPNLYRCTLTMEESVEGKGDAGLVQLFEDDQGLASGQFAAFYKGKTCLGS 426
            F+++QCKVRHGP  Y C+L ME   +G   + +V L EDDQGLA+GQFAAFY+GKTC+GS
Sbjct: 378  FNKLQCKVRHGPGFYDCSLIMETGEDGDRTSAVVHLSEDDQGLAAGQFAAFYQGKTCIGS 437

Query: 425  GIILESWEDQGFPVCARALEIARMEDKSKLGKPVKIMFKPEASITNSDHKDNVELPREHV 246
            G+ILE W+ QGFPVCA+AL+IARMEDKSKLGKPVKI   PE S+ +S+  D VEL  + +
Sbjct: 438  GVILECWDSQGFPVCAKALQIARMEDKSKLGKPVKIKVNPENSLKSSEITDKVELNGKSL 497

Query: 245  TKQVVASGTTDTQRLASRGVLSSVPLIWLQTLRSKWLRLF 126
              + +   T D  + +S+G+ +   + W Q LR KWL++F
Sbjct: 498  NSEDI---TLDKSKQSSQGIFN---VNWFQKLREKWLQVF 531


>ref|XP_010102337.1| tRNA-specific 2-thiouridylase mnmA [Morus notabilis]
            gi|587905107|gb|EXB93298.1| tRNA-specific 2-thiouridylase
            mnmA [Morus notabilis]
          Length = 580

 Score =  595 bits (1535), Expect = e-167
 Identities = 295/401 (73%), Positives = 326/401 (81%), Gaps = 1/401 (0%)
 Frame = -3

Query: 1325 QIGVPLEVVHLTDEYWSNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYV 1146
            QI VPLEVVHLTDEYW NVVSYII+EYRCGRTPNPDVLCNTRIKFG+FMDAIS+MEFDYV
Sbjct: 191  QIEVPLEVVHLTDEYWKNVVSYIIDEYRCGRTPNPDVLCNTRIKFGSFMDAISSMEFDYV 250

Query: 1145 ASGHYARLVHSSSDEDNERG-VSMLELSNDMVKDQTYFLSHLSQNQIKRLLFPLGCISKD 969
            ASGHYA +VHS +   NERG  S+LELS DMVKDQTYFLSHLSQ Q+KRL+FPLGCISK+
Sbjct: 251  ASGHYANVVHSFT---NERGDASVLELSQDMVKDQTYFLSHLSQAQLKRLVFPLGCISKE 307

Query: 968  EVRRLATKFDLPNKERKDSQGICFLGKIKFSEFVARHIGELEGVLLEAETGDFLGKHRGF 789
            EVR+LA KFDLPNK+RKDSQGICFLGKIKFSEFVARHIGE EG++LEAETGDFLG HRGF
Sbjct: 308  EVRKLAKKFDLPNKDRKDSQGICFLGKIKFSEFVARHIGEKEGIILEAETGDFLGMHRGF 367

Query: 788  WFYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYFSLDKKRRRFRVGSLKWLSGSPPD 609
            WFYTIGQRQGLRLPGGPWYVVEKD KNNVVF SRNYFS DK+RR FRVGSLKWL+G PPD
Sbjct: 368  WFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFASRNYFSFDKRRRIFRVGSLKWLNGLPPD 427

Query: 608  NFDEMQCKVRHGPNLYRCTLTMEESVEGKGDAGLVQLFEDDQGLASGQFAAFYKGKTCLG 429
               ++QCKVRHGP  Y C+ TME       D  +VQL EDDQGLA GQFAAFY+GK+CLG
Sbjct: 428  QTCQLQCKVRHGPGFYNCSFTMESGENSGEDVAVVQLSEDDQGLAVGQFAAFYQGKSCLG 487

Query: 428  SGIILESWEDQGFPVCARALEIARMEDKSKLGKPVKIMFKPEASITNSDHKDNVELPREH 249
            SGIILESW+DQGFPVC +ALEIA+MEDKS LGKPVKI  KPE+       K + EL R  
Sbjct: 488  SGIILESWDDQGFPVCEKALEIAKMEDKSLLGKPVKIKVKPESPTIEPGQKSSTELDRGQ 547

Query: 248  VTKQVVASGTTDTQRLASRGVLSSVPLIWLQTLRSKWLRLF 126
             +   +A+     Q L       S P+ WLQ LR KW R+F
Sbjct: 548  RSSH-IAAAKQGRQAL-------SFPMKWLQRLREKWFRIF 580


>gb|KDO61266.1| hypothetical protein CISIN_1g042717mg, partial [Citrus sinensis]
          Length = 474

 Score =  593 bits (1528), Expect = e-166
 Identities = 291/401 (72%), Positives = 330/401 (82%), Gaps = 2/401 (0%)
 Frame = -3

Query: 1325 QIGVPLEVVHLTDEYWSNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYV 1146
            Q+ VPLEVVHLTDEYW NVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAIS+MEFDYV
Sbjct: 80   QVDVPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISSMEFDYV 139

Query: 1145 ASGHYARLVHSSSDEDNERGVSMLELSNDMVKDQTYFLSHLSQNQIKRLLFPLGCISKDE 966
            ASGHYA++VHSS+D++ +   S+LELS D VKDQTYFLSHLSQ Q+KRL+FPLGCISK+E
Sbjct: 140  ASGHYAKVVHSSADQEYKP--SVLELSKDKVKDQTYFLSHLSQAQLKRLIFPLGCISKEE 197

Query: 965  VRRLATKFDLPNKERKDSQGICFLGKIKFSEFVARHIGELEGVLLEAETGDFLGKHRGFW 786
            VR+ AT+FDLPNK+RKDSQGICFLGKIKFSEFVARHIGE+EGV+LEAETGDFLGKH GFW
Sbjct: 198  VRKFATQFDLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGVILEAETGDFLGKHWGFW 257

Query: 785  FYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYFSLDKKRRRFRVGSLKWLSGSPPDN 606
            FYTIGQRQGLRLPGGPWYVVEKD KNNVVFVSRNY+S DKKRR FRVGSLKWLSG PP+ 
Sbjct: 258  FYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYYSFDKKRRLFRVGSLKWLSGLPPEE 317

Query: 605  FDEMQCKVRHGPNLYRCTLTMEESVEGKGDAGLVQLFEDDQGLASGQFAAFYKGKTCLGS 426
               +QCKVRHGP  Y C L +E   +G  +  +VQL EDDQGLA+GQFAAFY+G  C+GS
Sbjct: 318  ISRLQCKVRHGPGFYNCNLMIEHVEDGGENIAVVQLSEDDQGLAAGQFAAFYQGTRCIGS 377

Query: 425  GIILESWEDQGFPVCARALEIARMEDKSKLGKPVKIMFKPEASITNSDHKDNVELPREHV 246
            G+ILESW+DQGFPVC +ALEIARMEDKSKLGKP+KI  KPE     + +K  V+  R  V
Sbjct: 378  GVILESWDDQGFPVCEKALEIARMEDKSKLGKPIKIKVKPETPEEFNSNK-GVQSSRNLV 436

Query: 245  TKQ--VVASGTTDTQRLASRGVLSSVPLIWLQTLRSKWLRL 129
              Q  +     T +Q  A    +S  P+ WLQ LR KW R+
Sbjct: 437  NAQNSIAEKSNTTSQEEA----ISRFPMNWLQKLREKWPRI 473


>ref|XP_006470603.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like [Citrus
            sinensis]
          Length = 525

 Score =  593 bits (1528), Expect = e-166
 Identities = 291/401 (72%), Positives = 330/401 (82%), Gaps = 2/401 (0%)
 Frame = -3

Query: 1325 QIGVPLEVVHLTDEYWSNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYV 1146
            Q+ VPLEVVHLTDEYW NVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAIS+MEFDYV
Sbjct: 131  QVDVPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISSMEFDYV 190

Query: 1145 ASGHYARLVHSSSDEDNERGVSMLELSNDMVKDQTYFLSHLSQNQIKRLLFPLGCISKDE 966
            ASGHYA++VHSS+D++ +   S+LELS D VKDQTYFLSHLSQ Q+KRL+FPLGCISK+E
Sbjct: 191  ASGHYAKVVHSSADQEYKP--SVLELSKDKVKDQTYFLSHLSQAQLKRLIFPLGCISKEE 248

Query: 965  VRRLATKFDLPNKERKDSQGICFLGKIKFSEFVARHIGELEGVLLEAETGDFLGKHRGFW 786
            VR+ AT+FDLPNK+RKDSQGICFLGKIKFSEFVARHIGE+EGV+LEAETGDFLGKH GFW
Sbjct: 249  VRKFATQFDLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGVILEAETGDFLGKHWGFW 308

Query: 785  FYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYFSLDKKRRRFRVGSLKWLSGSPPDN 606
            FYTIGQRQGLRLPGGPWYVVEKD KNNVVFVSRNY+S DKKRR FRVGSLKWLSG PP+ 
Sbjct: 309  FYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYYSFDKKRRLFRVGSLKWLSGLPPEE 368

Query: 605  FDEMQCKVRHGPNLYRCTLTMEESVEGKGDAGLVQLFEDDQGLASGQFAAFYKGKTCLGS 426
               +QCKVRHGP  Y C L +E   +G  +  +VQL EDDQGLA+GQFAAFY+G  C+GS
Sbjct: 369  ISRLQCKVRHGPGFYNCNLMIEHVEDGGENIAVVQLSEDDQGLAAGQFAAFYQGTRCIGS 428

Query: 425  GIILESWEDQGFPVCARALEIARMEDKSKLGKPVKIMFKPEASITNSDHKDNVELPREHV 246
            G+ILESW+DQGFPVC +ALEIARMEDKSKLGKP+KI  KPE     + +K  V+  R  V
Sbjct: 429  GVILESWDDQGFPVCEKALEIARMEDKSKLGKPIKIKVKPETPEEFNSNK-GVQSSRNLV 487

Query: 245  TKQ--VVASGTTDTQRLASRGVLSSVPLIWLQTLRSKWLRL 129
              Q  +     T +Q  A    +S  P+ WLQ LR KW R+
Sbjct: 488  NAQNSIAEKSNTTSQEEA----ISRFPMNWLQKLREKWPRI 524


>ref|XP_008238179.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 isoform X1
            [Prunus mume]
          Length = 537

 Score =  591 bits (1523), Expect = e-166
 Identities = 288/400 (72%), Positives = 327/400 (81%)
 Frame = -3

Query: 1325 QIGVPLEVVHLTDEYWSNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYV 1146
            Q+ VPLEVVHLT+EYW NVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAIS M+FDYV
Sbjct: 142  QVDVPLEVVHLTNEYWKNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISCMKFDYV 201

Query: 1145 ASGHYARLVHSSSDEDNERGVSMLELSNDMVKDQTYFLSHLSQNQIKRLLFPLGCISKDE 966
            ASGHYA +VH S+ E ++   S+LELS DMVKDQTYFLSHLSQ Q+K+L+FPLGC+SKDE
Sbjct: 202  ASGHYANVVHPSAGEMDKD--SVLELSQDMVKDQTYFLSHLSQAQLKQLIFPLGCLSKDE 259

Query: 965  VRRLATKFDLPNKERKDSQGICFLGKIKFSEFVARHIGELEGVLLEAETGDFLGKHRGFW 786
            VR+LATKFDL NK+RKDSQGICFLGKIKFSEF+ARHIGE EG++LEAETGD LG HRGFW
Sbjct: 260  VRKLATKFDLLNKQRKDSQGICFLGKIKFSEFIARHIGEKEGIILEAETGDLLGTHRGFW 319

Query: 785  FYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYFSLDKKRRRFRVGSLKWLSGSPPDN 606
            FYTIGQRQGLRLPGGPWYVVEKD KNNVVFVSRNYFS+DK+RR FRVGSLKWL+G PP+ 
Sbjct: 320  FYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSVDKRRRLFRVGSLKWLNGFPPNQ 379

Query: 605  FDEMQCKVRHGPNLYRCTLTMEESVEGKGDAGLVQLFEDDQGLASGQFAAFYKGKTCLGS 426
              ++QCKVRHGP    C+L ME    G  D  +V L EDDQGLA+GQFAAFY+G+TCLGS
Sbjct: 380  ISQLQCKVRHGPGFRNCSLIMEPGAYGHEDVAVVHLSEDDQGLAAGQFAAFYQGRTCLGS 439

Query: 425  GIILESWEDQGFPVCARALEIARMEDKSKLGKPVKIMFKPEASITNSDHKDNVELPREHV 246
            G+ILESW+DQGFPVCARALEIARMEDKS LG+PVKI  KPE  IT SD  D +EL     
Sbjct: 440  GVILESWDDQGFPVCARALEIARMEDKSLLGRPVKIKVKPENPITESDQVDGMELCGG-- 497

Query: 245  TKQVVASGTTDTQRLASRGVLSSVPLIWLQTLRSKWLRLF 126
             K    S      +++    ++  P+ WLQ  R KWL +F
Sbjct: 498  LKHSGISAAKQRMQVSQGEAITRFPVNWLQKFREKWLHIF 537


>ref|XP_012446852.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 [Gossypium
            raimondii] gi|763788378|gb|KJB55374.1| hypothetical
            protein B456_009G073000 [Gossypium raimondii]
          Length = 490

 Score =  583 bits (1503), Expect = e-163
 Identities = 271/340 (79%), Positives = 308/340 (90%)
 Frame = -3

Query: 1325 QIGVPLEVVHLTDEYWSNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYV 1146
            Q+ VPL+VVHLTDEYW NVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAI NM++DY+
Sbjct: 149  QVDVPLQVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAIYNMDYDYI 208

Query: 1145 ASGHYARLVHSSSDEDNERGVSMLELSNDMVKDQTYFLSHLSQNQIKRLLFPLGCISKDE 966
            ASGHYA +VH S+D+ N+   S+LELS DMVKDQTYFLSHLSQ Q+K+L+FPLGC+SKDE
Sbjct: 209  ASGHYANVVHPSADQSNK--ASILELSQDMVKDQTYFLSHLSQFQLKQLIFPLGCLSKDE 266

Query: 965  VRRLATKFDLPNKERKDSQGICFLGKIKFSEFVARHIGELEGVLLEAETGDFLGKHRGFW 786
            VR+LA KFDLPNK RKDSQGICFLGKI+FS+FVARHIGE+EG++LEAETGDFLGKHRGFW
Sbjct: 267  VRQLAAKFDLPNKGRKDSQGICFLGKIRFSDFVARHIGEMEGIILEAETGDFLGKHRGFW 326

Query: 785  FYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYFSLDKKRRRFRVGSLKWLSGSPPDN 606
            FYTIGQRQGLRLPGGPWYVV+KD KNNVVFVSRNYFSLDK+RR FRVGSLKW SGSPP++
Sbjct: 327  FYTIGQRQGLRLPGGPWYVVQKDVKNNVVFVSRNYFSLDKRRRVFRVGSLKWFSGSPPNH 386

Query: 605  FDEMQCKVRHGPNLYRCTLTMEESVEGKGDAGLVQLFEDDQGLASGQFAAFYKGKTCLGS 426
             D++QCKVRHGP  Y C   ME   +G  D  +VQL EDDQGLA+GQFAAFY+G+TC+GS
Sbjct: 387  IDQLQCKVRHGPGFYNCNFKMEHGEDGNDDIAVVQLSEDDQGLAAGQFAAFYEGQTCIGS 446

Query: 425  GIILESWEDQGFPVCARALEIARMEDKSKLGKPVKIMFKP 306
            G+ILESW+D+GFPVCA+ALEIARMEDKS+LGKPVKIM KP
Sbjct: 447  GVILESWDDRGFPVCAKALEIARMEDKSELGKPVKIMTKP 486


>ref|XP_007209892.1| hypothetical protein PRUPE_ppa004048mg [Prunus persica]
            gi|462405627|gb|EMJ11091.1| hypothetical protein
            PRUPE_ppa004048mg [Prunus persica]
          Length = 533

 Score =  583 bits (1503), Expect = e-163
 Identities = 287/400 (71%), Positives = 325/400 (81%)
 Frame = -3

Query: 1325 QIGVPLEVVHLTDEYWSNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYV 1146
            Q+ VPLEVVHLT+EYW NVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAIS M+FDYV
Sbjct: 142  QVDVPLEVVHLTNEYWKNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISCMKFDYV 201

Query: 1145 ASGHYARLVHSSSDEDNERGVSMLELSNDMVKDQTYFLSHLSQNQIKRLLFPLGCISKDE 966
            ASGHYA +VH S+ E +E   S+LELS DM    TYFLSHLSQ Q+K+L+FPLGC+SKDE
Sbjct: 202  ASGHYANVVHPSAGEMDED--SVLELSQDM----TYFLSHLSQAQLKQLIFPLGCLSKDE 255

Query: 965  VRRLATKFDLPNKERKDSQGICFLGKIKFSEFVARHIGELEGVLLEAETGDFLGKHRGFW 786
            VR+LATKFDL NK+RKDSQGICFLGK+KFSEF+ARHIGE EG++LEAETGDFLG HRGFW
Sbjct: 256  VRKLATKFDLLNKQRKDSQGICFLGKLKFSEFIARHIGEKEGIILEAETGDFLGTHRGFW 315

Query: 785  FYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYFSLDKKRRRFRVGSLKWLSGSPPDN 606
            FYTIGQRQGLRLPGGPWYVVEKD KNNVVFVSRNYFS+DK+RR FRVGSLKWL+G P + 
Sbjct: 316  FYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSVDKRRRLFRVGSLKWLNGFPQNQ 375

Query: 605  FDEMQCKVRHGPNLYRCTLTMEESVEGKGDAGLVQLFEDDQGLASGQFAAFYKGKTCLGS 426
              ++QCKVRHGP    C+L ME    G  D  +V L EDDQGLA+GQFAAFY+G+TCLGS
Sbjct: 376  ISQLQCKVRHGPGFRNCSLIMEPGAYGHEDVAVVHLSEDDQGLAAGQFAAFYQGRTCLGS 435

Query: 425  GIILESWEDQGFPVCARALEIARMEDKSKLGKPVKIMFKPEASITNSDHKDNVELPREHV 246
            G+ILESW+DQGFPVCARALEIARMEDKS LGKPVKI  KPE  IT SD  D +EL R   
Sbjct: 436  GVILESWDDQGFPVCARALEIARMEDKSLLGKPVKIKVKPENPITESDQVDGMELCRG-- 493

Query: 245  TKQVVASGTTDTQRLASRGVLSSVPLIWLQTLRSKWLRLF 126
             K    S      +++    ++  P+ WLQ  R KWLR+F
Sbjct: 494  LKHSGISAAKQRMQVSQGEAITRFPVNWLQKFREKWLRIF 533


>gb|KHG00493.1| tRNA-specific 2-thiouridylase mnmA [Gossypium arboreum]
          Length = 490

 Score =  582 bits (1501), Expect = e-163
 Identities = 270/340 (79%), Positives = 309/340 (90%)
 Frame = -3

Query: 1325 QIGVPLEVVHLTDEYWSNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYV 1146
            Q+ VPL+VV LTDEYW+NVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAI NM++DY+
Sbjct: 149  QVDVPLQVVQLTDEYWNNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAIYNMDYDYI 208

Query: 1145 ASGHYARLVHSSSDEDNERGVSMLELSNDMVKDQTYFLSHLSQNQIKRLLFPLGCISKDE 966
            ASGHYA +VH S+D+ N+   S+LELS DMVKDQTYFLSHLSQ Q+K+L+FPLGC+SKDE
Sbjct: 209  ASGHYANVVHPSADQSNK--ASILELSQDMVKDQTYFLSHLSQFQLKQLIFPLGCLSKDE 266

Query: 965  VRRLATKFDLPNKERKDSQGICFLGKIKFSEFVARHIGELEGVLLEAETGDFLGKHRGFW 786
            VR+LA KFDLPNK RKDSQGICFLGKI+FS+FVARHIGE+EG++LEAETGDFLGKHRGFW
Sbjct: 267  VRQLAAKFDLPNKGRKDSQGICFLGKIRFSDFVARHIGEMEGIILEAETGDFLGKHRGFW 326

Query: 785  FYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYFSLDKKRRRFRVGSLKWLSGSPPDN 606
            FYTIGQRQGLRLPGGPWYVV+KD KNNVVFVSRNYFSLDK+RR FRVGSLKW SGSPP++
Sbjct: 327  FYTIGQRQGLRLPGGPWYVVQKDVKNNVVFVSRNYFSLDKRRRVFRVGSLKWFSGSPPNH 386

Query: 605  FDEMQCKVRHGPNLYRCTLTMEESVEGKGDAGLVQLFEDDQGLASGQFAAFYKGKTCLGS 426
             D++QCKVRHGP  Y C   ME   +G  D  +VQL+EDDQGLA+GQFAAFY+G+TC+GS
Sbjct: 387  IDQLQCKVRHGPGFYNCNFKMEHGEDGNDDIAVVQLYEDDQGLAAGQFAAFYEGQTCIGS 446

Query: 425  GIILESWEDQGFPVCARALEIARMEDKSKLGKPVKIMFKP 306
            G+ILESW+D+GFPVCA+ALEIARMEDKS+LGKPVKIM KP
Sbjct: 447  GVILESWDDRGFPVCAKALEIARMEDKSELGKPVKIMTKP 486


>ref|XP_002513510.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase,
            putative [Ricinus communis] gi|223547418|gb|EEF48913.1|
            tRNA
            (5-methylaminomethyl-2-thiouridylate)-methyltransferase,
            putative [Ricinus communis]
          Length = 497

 Score =  582 bits (1500), Expect = e-163
 Identities = 276/367 (75%), Positives = 313/367 (85%)
 Frame = -3

Query: 1325 QIGVPLEVVHLTDEYWSNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYV 1146
            Q+ +PLEVVHLTDEYW NVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAI+NMEFDYV
Sbjct: 132  QVDIPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAINNMEFDYV 191

Query: 1145 ASGHYARLVHSSSDEDNERGVSMLELSNDMVKDQTYFLSHLSQNQIKRLLFPLGCISKDE 966
            ASGHYA+++H S+DE N+   S+LELS DMVKDQTYFLSHLSQ Q++RL+FPLG ISKDE
Sbjct: 192  ASGHYAKVIHPSADEINQP--SVLELSKDMVKDQTYFLSHLSQAQLRRLIFPLGGISKDE 249

Query: 965  VRRLATKFDLPNKERKDSQGICFLGKIKFSEFVARHIGELEGVLLEAETGDFLGKHRGFW 786
            VR+LAT F+LPNK+RKDSQGICFLGKIKFSEFVARHIGE+EG++LEAETGDFLG HRGFW
Sbjct: 250  VRKLATLFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGIMLEAETGDFLGNHRGFW 309

Query: 785  FYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYFSLDKKRRRFRVGSLKWLSGSPPDN 606
            FYTIGQRQGLRLPGGPWYVVEKD KNNVVFVSRNYFS DK+RR  RVGSL+W +GSPP  
Sbjct: 310  FYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKRRRLLRVGSLRWFNGSPPKE 369

Query: 605  FDEMQCKVRHGPNLYRCTLTMEESVEGKGDAGLVQLFEDDQGLASGQFAAFYKGKTCLGS 426
              E+QCKVRHGP  Y C++ +E       D  +VQL EDDQGLA+GQFAAFYKG+TC+GS
Sbjct: 370  TSELQCKVRHGPGFYNCSVVIEPGEVDDQDVAIVQLCEDDQGLAAGQFAAFYKGRTCIGS 429

Query: 425  GIILESWEDQGFPVCARALEIARMEDKSKLGKPVKIMFKPEASITNSDHKDNVELPREHV 246
            G+ILESW DQGFPVC RALE+AR+EDKS+LGKPVKI  KPE  +   D KD +E+ R   
Sbjct: 430  GVILESWNDQGFPVCERALELARIEDKSELGKPVKIKVKPEIPVREFDGKDGIEVERGLN 489

Query: 245  TKQVVAS 225
              QV  +
Sbjct: 490  NSQVAVT 496


>ref|XP_002298404.2| hypothetical protein POPTR_0001s26620g [Populus trichocarpa]
            gi|550348245|gb|EEE83209.2| hypothetical protein
            POPTR_0001s26620g [Populus trichocarpa]
          Length = 512

 Score =  574 bits (1479), Expect = e-161
 Identities = 275/368 (74%), Positives = 312/368 (84%)
 Frame = -3

Query: 1325 QIGVPLEVVHLTDEYWSNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYV 1146
            Q+GVPLEVVHLTDEYWSNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAI+NMEFDYV
Sbjct: 147  QVGVPLEVVHLTDEYWSNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAINNMEFDYV 206

Query: 1145 ASGHYARLVHSSSDEDNERGVSMLELSNDMVKDQTYFLSHLSQNQIKRLLFPLGCISKDE 966
            ASGHYA ++H  SD+ N+   S+LELS D +KDQTYFLSHL+Q Q+KRL+FPLGCI K+E
Sbjct: 207  ASGHYAHVIHPVSDQINKP--SVLELSKDKLKDQTYFLSHLTQAQLKRLIFPLGCIPKEE 264

Query: 965  VRRLATKFDLPNKERKDSQGICFLGKIKFSEFVARHIGELEGVLLEAETGDFLGKHRGFW 786
            VR LA+KFDLPNK+RKDSQGICFLGKIKFSEFV RHIGE EGV+LEAETGDFLG HRGFW
Sbjct: 265  VRNLASKFDLPNKDRKDSQGICFLGKIKFSEFVGRHIGESEGVILEAETGDFLGIHRGFW 324

Query: 785  FYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYFSLDKKRRRFRVGSLKWLSGSPPDN 606
            FYTIGQRQGLRLPGGPWYVVEKD KNNVVFVSRNYFSLDK+RR FRVGSLKWLSGSP D 
Sbjct: 325  FYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSLDKRRRSFRVGSLKWLSGSPTDM 384

Query: 605  FDEMQCKVRHGPNLYRCTLTMEESVEGKGDAGLVQLFEDDQGLASGQFAAFYKGKTCLGS 426
              ++QCKVRHGP  Y C++ +E   +   +   V L EDDQGLA+GQF AFY+G+TC+GS
Sbjct: 385  TTQLQCKVRHGPTFYDCSVKIELGEDSPEEVVAVHLCEDDQGLAAGQFTAFYQGRTCIGS 444

Query: 425  GIILESWEDQGFPVCARALEIARMEDKSKLGKPVKIMFKPEASITNSDHKDNVELPREHV 246
            G+ILESW+D+GFPVC +ALE+AR+EDKSKLGKPVKI  KPE S   S  KD +E  +   
Sbjct: 445  GVILESWDDKGFPVCQKALELARLEDKSKLGKPVKIKVKPETSDQESVQKDGIEPDKGLA 504

Query: 245  TKQVVASG 222
              +V  +G
Sbjct: 505  EPRVAVTG 512


>ref|XP_011006202.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 [Populus
            euphratica]
          Length = 512

 Score =  573 bits (1476), Expect = e-160
 Identities = 272/353 (77%), Positives = 308/353 (87%)
 Frame = -3

Query: 1325 QIGVPLEVVHLTDEYWSNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYV 1146
            Q+GVPLEVVHLTDEYWSNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAF+DAI+NMEFDYV
Sbjct: 147  QVGVPLEVVHLTDEYWSNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFIDAINNMEFDYV 206

Query: 1145 ASGHYARLVHSSSDEDNERGVSMLELSNDMVKDQTYFLSHLSQNQIKRLLFPLGCISKDE 966
            ASGHYAR++H  S++ N+   S+LELS D +KDQTYFLSHL+Q Q+KRL+FPLGCI K+E
Sbjct: 207  ASGHYARVIHPVSNQINKP--SVLELSKDKLKDQTYFLSHLAQAQLKRLIFPLGCIPKEE 264

Query: 965  VRRLATKFDLPNKERKDSQGICFLGKIKFSEFVARHIGELEGVLLEAETGDFLGKHRGFW 786
            VR LA+KFDLPNK+RKDSQGICFLGKIKFSEFV RHIGE EGV+LEAETGDFLG HRGFW
Sbjct: 265  VRNLASKFDLPNKDRKDSQGICFLGKIKFSEFVVRHIGESEGVILEAETGDFLGIHRGFW 324

Query: 785  FYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYFSLDKKRRRFRVGSLKWLSGSPPDN 606
            FYTIGQRQGLRLPGGPWYVVEKD KNNVVFVSRNYFSLDK+RR FRVGSLKWLSGSPPD 
Sbjct: 325  FYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSLDKRRRSFRVGSLKWLSGSPPDM 384

Query: 605  FDEMQCKVRHGPNLYRCTLTMEESVEGKGDAGLVQLFEDDQGLASGQFAAFYKGKTCLGS 426
              + QCKVRHGP  Y C++ +E   +   +  +V L EDDQGLA+GQF AFY+G+TC+GS
Sbjct: 385  TSQFQCKVRHGPTFYDCSVKIELGEDSPEEVVVVHLCEDDQGLAAGQFTAFYQGRTCIGS 444

Query: 425  GIILESWEDQGFPVCARALEIARMEDKSKLGKPVKIMFKPEASITNSDHKDNV 267
            G+ILESW+D+GFPVC +ALE+AR+EDKSKLGKPVKI  KPE S   S  KD +
Sbjct: 445  GVILESWDDKGFPVCQKALELARLEDKSKLGKPVKIKVKPETSDQESVQKDGI 497


>ref|XP_004507093.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 [Cicer
            arietinum]
          Length = 484

 Score =  572 bits (1473), Expect = e-160
 Identities = 270/341 (79%), Positives = 304/341 (89%)
 Frame = -3

Query: 1325 QIGVPLEVVHLTDEYWSNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYV 1146
            Q+ VPLEVVHLTDEYW NVVSYIIEEY+CGRTPNPDVLCNTRIKFGAF+DAIS+M FDYV
Sbjct: 130  QVEVPLEVVHLTDEYWKNVVSYIIEEYQCGRTPNPDVLCNTRIKFGAFLDAISDMGFDYV 189

Query: 1145 ASGHYARLVHSSSDEDNERGVSMLELSNDMVKDQTYFLSHLSQNQIKRLLFPLGCISKDE 966
            ASGHYA ++H S+D+ +  G S LELS DMVKDQTYFLSHLSQ+Q+K+LLFPLGCI KDE
Sbjct: 190  ASGHYANVIHPSADQMD--GPSTLELSQDMVKDQTYFLSHLSQSQLKQLLFPLGCIPKDE 247

Query: 965  VRRLATKFDLPNKERKDSQGICFLGKIKFSEFVARHIGELEGVLLEAETGDFLGKHRGFW 786
            VRRLATKFDLPNK+RKDSQGICFLGKIKFSEFVARHIGE EG++LEAETGDFLGKHRGFW
Sbjct: 248  VRRLATKFDLPNKDRKDSQGICFLGKIKFSEFVARHIGEKEGIILEAETGDFLGKHRGFW 307

Query: 785  FYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYFSLDKKRRRFRVGSLKWLSGSPPDN 606
            FYTIGQRQGLRLPGGPWYVVEKD KNNVVFVSRNYFS+DK+RR FRVGSLKWLSG PP  
Sbjct: 308  FYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSIDKRRRVFRVGSLKWLSGLPPSQ 367

Query: 605  FDEMQCKVRHGPNLYRCTLTMEESVEGKGDAGLVQLFEDDQGLASGQFAAFYKGKTCLGS 426
              +++CKVRHGP  Y C+L ME   E   D+ +V++ EDDQGLA+GQF AFY+G+TC+GS
Sbjct: 368  TSQLRCKVRHGPGFYDCSLQMELEGENPIDSAVVRISEDDQGLAAGQFTAFYEGRTCIGS 427

Query: 425  GIILESWEDQGFPVCARALEIARMEDKSKLGKPVKIMFKPE 303
            G+ILESW+DQGFPVC +ALEIARMEDKSK+G PVKI  KP+
Sbjct: 428  GVILESWDDQGFPVCTKALEIARMEDKSKIGNPVKIKVKPD 468


>ref|XP_010045014.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 isoform X1
            [Eucalyptus grandis]
          Length = 495

 Score =  571 bits (1471), Expect = e-160
 Identities = 268/344 (77%), Positives = 306/344 (88%)
 Frame = -3

Query: 1325 QIGVPLEVVHLTDEYWSNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYV 1146
            Q+ VPLEVVHLTDEYW NVVSYIIEEYR GRTPNPDVLCNTRIKFGAFMDAI++ME+D++
Sbjct: 154  QVDVPLEVVHLTDEYWKNVVSYIIEEYRSGRTPNPDVLCNTRIKFGAFMDAINSMEYDFI 213

Query: 1145 ASGHYARLVHSSSDEDNERGVSMLELSNDMVKDQTYFLSHLSQNQIKRLLFPLGCISKDE 966
            ASGHYA++VHSS+  DN    S L+LS DMVKDQTYFLSHLSQ Q+KRL+FPLGCISK+E
Sbjct: 214  ASGHYAKVVHSSNHIDN---ASELKLSEDMVKDQTYFLSHLSQAQLKRLIFPLGCISKNE 270

Query: 965  VRRLATKFDLPNKERKDSQGICFLGKIKFSEFVARHIGELEGVLLEAETGDFLGKHRGFW 786
            VR+LA +FDLPN++RKDSQGICFLGKIKFSEFVARHIGE+EG++LEAETGDFLG HRGFW
Sbjct: 271  VRKLAARFDLPNQDRKDSQGICFLGKIKFSEFVARHIGEMEGIILEAETGDFLGNHRGFW 330

Query: 785  FYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYFSLDKKRRRFRVGSLKWLSGSPPDN 606
            FYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYFS+DK+RR FRVGSLKWL G+PP  
Sbjct: 331  FYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYFSVDKRRRLFRVGSLKWLIGAPPTE 390

Query: 605  FDEMQCKVRHGPNLYRCTLTMEESVEGKGDAGLVQLFEDDQGLASGQFAAFYKGKTCLGS 426
              ++QCKVRHGP  Y C+L++E    G+ D  +VQL EDDQGLA+GQF AFY+ +TC+GS
Sbjct: 391  ISQLQCKVRHGPGFYNCSLSLELGENGQEDVAVVQLPEDDQGLAAGQFTAFYQDRTCIGS 450

Query: 425  GIILESWEDQGFPVCARALEIARMEDKSKLGKPVKIMFKPEASI 294
            G+ILESW DQGFPVC +ALEIARMEDKSKLGKPVKI  KPE ++
Sbjct: 451  GVILESWNDQGFPVCQKALEIARMEDKSKLGKPVKIKVKPEGTV 494


>gb|KCW87153.1| hypothetical protein EUGRSUZ_B03681 [Eucalyptus grandis]
          Length = 408

 Score =  571 bits (1471), Expect = e-160
 Identities = 268/344 (77%), Positives = 306/344 (88%)
 Frame = -3

Query: 1325 QIGVPLEVVHLTDEYWSNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYV 1146
            Q+ VPLEVVHLTDEYW NVVSYIIEEYR GRTPNPDVLCNTRIKFGAFMDAI++ME+D++
Sbjct: 67   QVDVPLEVVHLTDEYWKNVVSYIIEEYRSGRTPNPDVLCNTRIKFGAFMDAINSMEYDFI 126

Query: 1145 ASGHYARLVHSSSDEDNERGVSMLELSNDMVKDQTYFLSHLSQNQIKRLLFPLGCISKDE 966
            ASGHYA++VHSS+  DN    S L+LS DMVKDQTYFLSHLSQ Q+KRL+FPLGCISK+E
Sbjct: 127  ASGHYAKVVHSSNHIDN---ASELKLSEDMVKDQTYFLSHLSQAQLKRLIFPLGCISKNE 183

Query: 965  VRRLATKFDLPNKERKDSQGICFLGKIKFSEFVARHIGELEGVLLEAETGDFLGKHRGFW 786
            VR+LA +FDLPN++RKDSQGICFLGKIKFSEFVARHIGE+EG++LEAETGDFLG HRGFW
Sbjct: 184  VRKLAARFDLPNQDRKDSQGICFLGKIKFSEFVARHIGEMEGIILEAETGDFLGNHRGFW 243

Query: 785  FYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYFSLDKKRRRFRVGSLKWLSGSPPDN 606
            FYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYFS+DK+RR FRVGSLKWL G+PP  
Sbjct: 244  FYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYFSVDKRRRLFRVGSLKWLIGAPPTE 303

Query: 605  FDEMQCKVRHGPNLYRCTLTMEESVEGKGDAGLVQLFEDDQGLASGQFAAFYKGKTCLGS 426
              ++QCKVRHGP  Y C+L++E    G+ D  +VQL EDDQGLA+GQF AFY+ +TC+GS
Sbjct: 304  ISQLQCKVRHGPGFYNCSLSLELGENGQEDVAVVQLPEDDQGLAAGQFTAFYQDRTCIGS 363

Query: 425  GIILESWEDQGFPVCARALEIARMEDKSKLGKPVKIMFKPEASI 294
            G+ILESW DQGFPVC +ALEIARMEDKSKLGKPVKI  KPE ++
Sbjct: 364  GVILESWNDQGFPVCQKALEIARMEDKSKLGKPVKIKVKPEGTV 407


>ref|XP_011045440.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1-like
            [Populus euphratica]
          Length = 512

 Score =  570 bits (1469), Expect = e-159
 Identities = 271/353 (76%), Positives = 307/353 (86%)
 Frame = -3

Query: 1325 QIGVPLEVVHLTDEYWSNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYV 1146
            Q+GVPLEVVHLTDEYWSNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAF+DAI+NMEFDYV
Sbjct: 147  QVGVPLEVVHLTDEYWSNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFIDAINNMEFDYV 206

Query: 1145 ASGHYARLVHSSSDEDNERGVSMLELSNDMVKDQTYFLSHLSQNQIKRLLFPLGCISKDE 966
            ASGHYAR++H  S++ N+   S+LELS D +KDQTYFLSHL+Q Q+KRL+FPLGCI K+E
Sbjct: 207  ASGHYARVIHPVSNQINKP--SVLELSKDKLKDQTYFLSHLAQAQLKRLIFPLGCIPKEE 264

Query: 965  VRRLATKFDLPNKERKDSQGICFLGKIKFSEFVARHIGELEGVLLEAETGDFLGKHRGFW 786
            VR LA+KFDLPNK+RKDSQGICFLGKIKFSEFV RHIGE EGV+LEAETGDFLG HRGFW
Sbjct: 265  VRNLASKFDLPNKDRKDSQGICFLGKIKFSEFVVRHIGESEGVILEAETGDFLGIHRGFW 324

Query: 785  FYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYFSLDKKRRRFRVGSLKWLSGSPPDN 606
            FYTIGQRQGLRLPGGPWYVVEKD KNNVVFVSRNYFSLDK+R  FRVGSLKWLSGSPPD 
Sbjct: 325  FYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSLDKRRLSFRVGSLKWLSGSPPDM 384

Query: 605  FDEMQCKVRHGPNLYRCTLTMEESVEGKGDAGLVQLFEDDQGLASGQFAAFYKGKTCLGS 426
              + QCKVRHGP  Y C++ +E   +   +  +V L EDDQGLA+GQF AFY+G+TC+GS
Sbjct: 385  TSQFQCKVRHGPTFYDCSVKIELGEDSPEEVVVVHLCEDDQGLAAGQFTAFYQGRTCIGS 444

Query: 425  GIILESWEDQGFPVCARALEIARMEDKSKLGKPVKIMFKPEASITNSDHKDNV 267
            G+ILESW+D+GFPVC +ALE+AR+EDKSKLGKPVKI  KPE S   S  KD +
Sbjct: 445  GVILESWDDKGFPVCQKALELARLEDKSKLGKPVKIKVKPETSDQESVQKDGI 497


>ref|XP_010326622.1| PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 [Solanum
            lycopersicum]
          Length = 484

 Score =  569 bits (1467), Expect = e-159
 Identities = 272/345 (78%), Positives = 306/345 (88%)
 Frame = -3

Query: 1325 QIGVPLEVVHLTDEYWSNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYV 1146
            Q+ VPLEVVHLTDEYW+NVVSYIIEEY+CGRTPNPDVLCNTRIKFGAFMDAIS MEFD+V
Sbjct: 141  QVDVPLEVVHLTDEYWNNVVSYIIEEYKCGRTPNPDVLCNTRIKFGAFMDAISGMEFDFV 200

Query: 1145 ASGHYARLVHSSSDEDNERGVSMLELSNDMVKDQTYFLSHLSQNQIKRLLFPLGCISKDE 966
            ASGHYA++VH+S+++ +E   S+LELS DMVKDQTYFLSHLSQ Q+KRL+FPLGCI KDE
Sbjct: 201  ASGHYAKIVHASTEQLDEP--SVLELSKDMVKDQTYFLSHLSQAQLKRLIFPLGCIQKDE 258

Query: 965  VRRLATKFDLPNKERKDSQGICFLGKIKFSEFVARHIGELEGVLLEAETGDFLGKHRGFW 786
            VR LA  F+LPN+ERKDSQGICFLGKIKFSEFVARHIGE EG++LEAE GD+LG HRGFW
Sbjct: 259  VRMLAKSFNLPNQERKDSQGICFLGKIKFSEFVARHIGESEGIILEAENGDYLGNHRGFW 318

Query: 785  FYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYFSLDKKRRRFRVGSLKWLSGSPPDN 606
            FYTIGQRQGLRLPGGPWYVVEKD KNNVV+VSRNYFS+DKKRR FRVGSLKWLSG  P  
Sbjct: 319  FYTIGQRQGLRLPGGPWYVVEKDIKNNVVYVSRNYFSVDKKRRLFRVGSLKWLSGLFPKQ 378

Query: 605  FDEMQCKVRHGPNLYRCTLTMEESVEGKGDAGLVQLFEDDQGLASGQFAAFYKGKTCLGS 426
             DE+QCKVRHGP  Y C+L ME    G+ +  +V+L  DDQGLA+GQFAAFY G+TC+GS
Sbjct: 379  IDELQCKVRHGPGFYNCSLVMEVDQHGQ-EVAVVRLSGDDQGLAAGQFAAFYDGRTCIGS 437

Query: 425  GIILESWEDQGFPVCARALEIARMEDKSKLGKPVKIMFKPEASIT 291
            GIILESW+DQG+P+C RALEIARMEDKSKLGKPVKIM KPE S+T
Sbjct: 438  GIILESWDDQGYPICERALEIARMEDKSKLGKPVKIMVKPELSVT 482


>gb|KNA16767.1| hypothetical protein SOVF_086320 [Spinacia oleracea]
          Length = 526

 Score =  569 bits (1466), Expect = e-159
 Identities = 285/408 (69%), Positives = 329/408 (80%), Gaps = 8/408 (1%)
 Frame = -3

Query: 1325 QIGVPLEVVHLTDEYWSNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYV 1146
            Q+ +PLEVVHLTDEYW  VVSYIIEEYR GRTPNPDVLCNTRIKFGAFMDAIS+M FDYV
Sbjct: 139  QVDIPLEVVHLTDEYWEKVVSYIIEEYRNGRTPNPDVLCNTRIKFGAFMDAISSMNFDYV 198

Query: 1145 ASGHYARLVHSSSDE-DNERGVSMLELSNDMVKDQTYFLSHLSQNQIKRLLFPLGCISKD 969
            ASGHYA ++H S++  DN    S+L+LS DMVKDQTYFLSHL Q+Q+KRL+FPLGCISKD
Sbjct: 199  ASGHYANVIHPSTEALDNP---SVLQLSRDMVKDQTYFLSHLDQSQLKRLIFPLGCISKD 255

Query: 968  EVRRLATKFDLPNKERKDSQGICFLGKIKFSEFVARHIGELEGVLLEAETGDFLGKHRGF 789
            EVR+LA+KF LPNK+RKDSQGICFLGKIKFS+FV RHIGE+EGV+LEAETGDFLG+HRGF
Sbjct: 256  EVRKLASKFALPNKDRKDSQGICFLGKIKFSDFVTRHIGEMEGVMLEAETGDFLGQHRGF 315

Query: 788  WFYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYFSLDKKRRRFRVGSLKWLSGSPPD 609
            WFYTIGQRQGLRLPGGPWYVV+KD KNNVVFVSRNY+S+DK+RR FRVGSLKWL G PPD
Sbjct: 316  WFYTIGQRQGLRLPGGPWYVVQKDVKNNVVFVSRNYYSVDKRRRVFRVGSLKWL-GLPPD 374

Query: 608  NFDEMQCKVRHGPNLYRCTLTMEESVEGKGDAGLVQLFEDDQGLASGQFAAFYKGKTCLG 429
            N  E+QCKVRHGPN Y C+LTM       GD  +V L EDDQGLA+GQFAAFY+G+TC+G
Sbjct: 375  NMSELQCKVRHGPNFYCCSLTMVSGENCNGDVAVVHLSEDDQGLAAGQFAAFYQGRTCVG 434

Query: 428  SGIILESW-EDQGFPVCARALEIARMEDKSKLGKPVKIMFKPEASITNSDHKDNVELPRE 252
            SGIILESW E++GFPVC +ALEIARMEDKSKLGKPVKI  KPE      + K        
Sbjct: 435  SGIILESWDEEEGFPVCEKALEIARMEDKSKLGKPVKIKVKPEDPQVGLNEK-------- 486

Query: 251  HVTKQVVASGTTDTQRLASRGVLS------SVPLIWLQTLRSKWLRLF 126
                    +G+T++ ++ +RGV S       V   W+Q L+ K L+LF
Sbjct: 487  --------AGSTNSSKVTTRGVESEEKVTDGVSPNWMQQLKQKILQLF 526