BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Aconitum23_contig00008054
         (2293 letters)

Database: ./nr 
           77,306,371 sequences; 28,104,191,420 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_010263294.1| PREDICTED: isoamylase 1, chloroplastic [Nelu...   920   0.0  
ref|XP_007014466.1| Isoamylase 1 isoform 2 [Theobroma cacao] gi|...   908   0.0  
ref|XP_002265964.3| PREDICTED: isoamylase 1, chloroplastic [Viti...   907   0.0  
emb|CBI40669.3| unnamed protein product [Vitis vinifera]              900   0.0  
ref|XP_012459147.1| PREDICTED: isoamylase 1, chloroplastic isofo...   895   0.0  
ref|XP_006474309.1| PREDICTED: isoamylase 1, chloroplastic-like ...   895   0.0  
gb|KDO61988.1| hypothetical protein CISIN_1g003653mg [Citrus sin...   894   0.0  
ref|XP_011020102.1| PREDICTED: isoamylase 1, chloroplastic-like ...   892   0.0  
ref|XP_011020101.1| PREDICTED: isoamylase 1, chloroplastic-like ...   892   0.0  
ref|XP_002308090.2| isoamylase family protein [Populus trichocar...   891   0.0  
ref|XP_006381137.1| hypothetical protein POPTR_0006s07010g [Popu...   891   0.0  
ref|XP_008223817.1| PREDICTED: isoamylase 1, chloroplastic [Prun...   886   0.0  
ref|XP_002324659.2| isoamylase family protein [Populus trichocar...   882   0.0  
ref|XP_011017747.1| PREDICTED: isoamylase 1, chloroplastic-like ...   881   0.0  
ref|XP_011017745.1| PREDICTED: isoamylase 1, chloroplastic-like ...   879   0.0  
ref|NP_001274937.1| isoamylase isoform 1 [Solanum tuberosum] gi|...   877   0.0  
ref|XP_009588914.1| PREDICTED: isoamylase 1, chloroplastic [Nico...   875   0.0  
ref|XP_009771255.1| PREDICTED: isoamylase 1, chloroplastic [Nico...   875   0.0  
ref|XP_010688426.1| PREDICTED: isoamylase 1, chloroplastic [Beta...   874   0.0  
ref|XP_004242868.1| PREDICTED: isoamylase 1, chloroplastic isofo...   869   0.0  

>ref|XP_010263294.1| PREDICTED: isoamylase 1, chloroplastic [Nelumbo nucifera]
          Length = 806

 Score =  920 bits (2379), Expect(2) = 0.0
 Identities = 428/521 (82%), Positives = 470/521 (90%), Gaps = 1/521 (0%)
 Frame = -2

Query: 2265 ETVVIEKPESRRFQVFNGRPLPFGATVRDGGVNFAIHSSGAVSATLCLISLSTLQENRVT 2086
            ET V+EKP  RRF+VF G P+PFGAT RDGGVNFAI+S  A SATLCLISLS LQEN+VT
Sbjct: 77   ETAVVEKPRLRRFEVFAGSPMPFGATARDGGVNFAIYSGHATSATLCLISLSDLQENKVT 136

Query: 2085 KKINLDPLINKTGDVWHVFLKGDFENMLYGYTFDGEFSPEEGYCYDSSKILLDPYAKAVI 1906
            ++I LDPLINKTGDVWHV+LKGDFE+MLYGY FDG+FSPEEG+ YDSS+ILLDPYAKAVI
Sbjct: 137  EQICLDPLINKTGDVWHVYLKGDFEDMLYGYKFDGKFSPEEGHYYDSSRILLDPYAKAVI 196

Query: 1905 SREEFGALGSEGNCWSQMAGMVPYNDNEFNWEGDLPLRHPQRDLIVYEMHVRGFTRHDSS 1726
            SR E+GALG EG+CW QMAG++P +DNEF+WEGDLPL+HPQRDLI+YEMHVRGFT HDSS
Sbjct: 197  SRGEYGALGPEGSCWPQMAGIIPSSDNEFDWEGDLPLKHPQRDLIIYEMHVRGFTMHDSS 256

Query: 1725 ETDFPGTYLGVVEKLDHLKELGVNCIELMPFHEYNELEYFSYNSILGNYKLNFWGYSTVN 1546
            ++ FPGTY+GVVEKLDHLKELG+NCIELMP  EYNELEYFSYNS+LG+YKLNFWGYSTVN
Sbjct: 257  KSRFPGTYIGVVEKLDHLKELGINCIELMPCQEYNELEYFSYNSVLGDYKLNFWGYSTVN 316

Query: 1545 FFSPMLRYSSAGIRNCGQDAINEVKILVREAHKRGIEVLMDVVFNHTAEGNEKGPMLSFR 1366
            +FSPM+RYSS GI NCG+DAINEVK L+REAHKRGIEVL+DVVFNHTAEGNE GP+LSFR
Sbjct: 317  YFSPMIRYSSGGILNCGRDAINEVKTLIREAHKRGIEVLLDVVFNHTAEGNENGPVLSFR 376

Query: 1365 GVDNSVYYMLAPKGEYYNYSGCGNTFNCNHPVVRQFIVDCLRYWVEEMHVDGFRFDLASI 1186
            GVDNS YYMLAPKGE+YNYSGCGNTFNCNHPVVRQFI+DCLRYWV EMHVDGFRFDLASI
Sbjct: 377  GVDNSAYYMLAPKGEFYNYSGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLASI 436

Query: 1185 LTRSSSLWDAVNVYGNSTEGDMLTTGTPLGSPPLVEMISNDPILRHVKLIAEAWDTGGLY 1006
            LTR  SLWDAVNVYGN  EGD+LTTGTPLG+PPL++MISNDPIL  VKLIAEAWDTGGLY
Sbjct: 437  LTRGCSLWDAVNVYGNPIEGDLLTTGTPLGNPPLIDMISNDPILCGVKLIAEAWDTGGLY 496

Query: 1005 QVGVFPHWGIWSEWNGKYRDIVRQFIKGTGGLSGAFAECLCGSPNLYQ-GARKPWNSINF 829
            QVG FPHWGIWSEWNGKYRDIVRQFIKGT G SG+FAEC+CGSPNLYQ   RKPWNSINF
Sbjct: 497  QVGTFPHWGIWSEWNGKYRDIVRQFIKGTDGFSGSFAECICGSPNLYQERGRKPWNSINF 556

Query: 828  VCAHDGFTLADLVTYNDKHNLAXXXXXXXXXXXXNSWNCGQ 706
            +CAHDGFTLADLVTYN+KHNLA            NSWNCGQ
Sbjct: 557  ICAHDGFTLADLVTYNNKHNLANGEDNNDGENHNNSWNCGQ 597



 Score =  334 bits (857), Expect(2) = 0.0
 Identities = 148/182 (81%), Positives = 173/182 (95%)
 Frame = -3

Query: 716  IVVRGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKQESSSDFFRFCSLLTKFRRE 537
            +V +GVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKK+ESSSDFFRFC L+T FRRE
Sbjct: 622  MVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKEESSSDFFRFCRLMTTFRRE 681

Query: 536  CESLGLDNFPTADRLQWHGHFPGVPDWSETSRFVAFSLIDSVKRELYIAFNTSHLPVIIT 357
            CESLGL++FPTA+RLQWHGH P +PDWS+TSRFVAFSL+D+VK E+Y+AFNTSHLPV++T
Sbjct: 682  CESLGLNDFPTAERLQWHGHAPRMPDWSDTSRFVAFSLVDAVKGEIYVAFNTSHLPVMVT 741

Query: 356  LPKRPGYRWEPLVDTSKTSPFDFLSDDLPEKAIALEQYSHFLNADVYPMLSYSSIILLLT 177
            LP+RPGYRWEPLVDTSK SPFDFLS+DLPE+ +A++QYSHFL+A++YPMLSYSSIIL L+
Sbjct: 742  LPERPGYRWEPLVDTSKPSPFDFLSEDLPERDVAIKQYSHFLDANLYPMLSYSSIILTLS 801

Query: 176  PD 171
            PD
Sbjct: 802  PD 803


>ref|XP_007014466.1| Isoamylase 1 isoform 2 [Theobroma cacao] gi|508784829|gb|EOY32085.1|
            Isoamylase 1 isoform 2 [Theobroma cacao]
          Length = 795

 Score =  908 bits (2347), Expect(2) = 0.0
 Identities = 423/522 (81%), Positives = 471/522 (90%), Gaps = 1/522 (0%)
 Frame = -2

Query: 2268 AETVVIEKPESRRFQVFNGRPLPFGATVRDGGVNFAIHSSGAVSATLCLISLSTLQENRV 2089
            A+T V+EKP+ +RFQ+  G P PFGAT+ DGGVNFAI+S+ AVSATLCLI+ S LQ+NRV
Sbjct: 65   ADTAVVEKPKIKRFQLSEGHPGPFGATLLDGGVNFAIYSANAVSATLCLITFSDLQQNRV 124

Query: 2088 TKKINLDPLINKTGDVWHVFLKGDFENMLYGYTFDGEFSPEEGYCYDSSKILLDPYAKAV 1909
            T++I+LDPL NKTGDVWHVFL+GDF++MLYGY FDG+FSPE+G+ YDSSKILLDPYAKAV
Sbjct: 125  TEQISLDPLTNKTGDVWHVFLRGDFKDMLYGYIFDGKFSPEKGHYYDSSKILLDPYAKAV 184

Query: 1908 ISREEFGALGSEGNCWSQMAGMVPYNDNEFNWEGDLPLRHPQRDLIVYEMHVRGFTRHDS 1729
            ISR EFGALG E NCW QMA MVP +++EF+WEGDLPLR+ QRDLI+YEMHVRGFTRH+S
Sbjct: 185  ISRGEFGALGPEDNCWPQMACMVPTSEDEFDWEGDLPLRYSQRDLIIYEMHVRGFTRHES 244

Query: 1728 SETDFPGTYLGVVEKLDHLKELGVNCIELMPFHEYNELEYFSYNSILGNYKLNFWGYSTV 1549
            S T+FPGTY GVVEKLDHLKELGVNCIELMP HE+NELEY+SYNSILG+YKLNFWGYSTV
Sbjct: 245  SRTEFPGTYRGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSILGDYKLNFWGYSTV 304

Query: 1548 NFFSPMLRYSSAGIRNCGQDAINEVKILVREAHKRGIEVLMDVVFNHTAEGNEKGPMLSF 1369
            NFFSPM+RYSS+G+ NCG+DA+NE K LV+EAHKRGIEV MDVVFNHTAEGNE GP+LSF
Sbjct: 305  NFFSPMIRYSSSGMHNCGRDAVNEFKYLVKEAHKRGIEVFMDVVFNHTAEGNENGPILSF 364

Query: 1368 RGVDNSVYYMLAPKGEYYNYSGCGNTFNCNHPVVRQFIVDCLRYWVEEMHVDGFRFDLAS 1189
            RGVDNSVYYMLAPKGEYYNYSGCGNTFNCNHPVVRQFI+DCLRYWV EMHVDGFRFDLAS
Sbjct: 365  RGVDNSVYYMLAPKGEYYNYSGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDLAS 424

Query: 1188 ILTRSSSLWDAVNVYGNSTEGDMLTTGTPLGSPPLVEMISNDPILRHVKLIAEAWDTGGL 1009
            I+TRSSSLWD VNV+GN  +GD++TTGTPL SPPL++M+SNDP+LR VKLIAEAWDTGGL
Sbjct: 425  IMTRSSSLWDLVNVFGNPLKGDLITTGTPLSSPPLIDMMSNDPVLRGVKLIAEAWDTGGL 484

Query: 1008 YQVGVFPHWGIWSEWNGKYRDIVRQFIKGTGGLSGAFAECLCGSPNLYQ-GARKPWNSIN 832
            YQVG FPHWGIWSEWNGKYRDIVRQFIKGT G SGAFAECLCGSPNLYQ G RKPWNSIN
Sbjct: 485  YQVGSFPHWGIWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQEGGRKPWNSIN 544

Query: 831  FVCAHDGFTLADLVTYNDKHNLAXXXXXXXXXXXXNSWNCGQ 706
            FVCAHDGFTLADLVTYN+KHNLA            NSWNCGQ
Sbjct: 545  FVCAHDGFTLADLVTYNNKHNLANGEDNNDGESHNNSWNCGQ 586



 Score =  320 bits (819), Expect(2) = 0.0
 Identities = 143/184 (77%), Positives = 170/184 (92%)
 Frame = -3

Query: 716  IVVRGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKQESSSDFFRFCSLLTKFRRE 537
            +V +G+PMI MGDEYGHTKGGNNNTYCHDNYINYF+WDKK+E SSDFFRFC L+TKFRRE
Sbjct: 611  MVSQGIPMICMGDEYGHTKGGNNNTYCHDNYINYFQWDKKEECSSDFFRFCCLMTKFRRE 670

Query: 536  CESLGLDNFPTADRLQWHGHFPGVPDWSETSRFVAFSLIDSVKRELYIAFNTSHLPVIIT 357
            CESLGL++FPTA+RLQWHGH PG+PDWS+ SRFVAF+LIDSVK E+Y+AFNTS+LPV IT
Sbjct: 671  CESLGLNDFPTAERLQWHGHTPGMPDWSDRSRFVAFTLIDSVKGEIYVAFNTSYLPVTIT 730

Query: 356  LPKRPGYRWEPLVDTSKTSPFDFLSDDLPEKAIALEQYSHFLNADVYPMLSYSSIILLLT 177
            LP+ PGYRWEPLVDTSK +PFDFLS++L E+ IA++QY  FL+A++YPMLSYSSIILLL+
Sbjct: 731  LPEHPGYRWEPLVDTSKPAPFDFLSEELKERDIAIKQYVQFLDANLYPMLSYSSIILLLS 790

Query: 176  PDGD 165
            PDG+
Sbjct: 791  PDGN 794


>ref|XP_002265964.3| PREDICTED: isoamylase 1, chloroplastic [Vitis vinifera]
          Length = 793

 Score =  907 bits (2344), Expect(2) = 0.0
 Identities = 422/526 (80%), Positives = 468/526 (88%), Gaps = 1/526 (0%)
 Frame = -2

Query: 2280 TGEVAETVVIEKPESRRFQVFNGRPLPFGATVRDGGVNFAIHSSGAVSATLCLISLSTLQ 2101
            +G  AETVV+EKP+ + F VF G P P GAT RDGGVNFA++S  AVSATLCLIS S L+
Sbjct: 58   SGAEAETVVVEKPKLQPFLVFEGCPAPLGATARDGGVNFAVYSGNAVSATLCLISASDLE 117

Query: 2100 ENRVTKKINLDPLINKTGDVWHVFLKGDFENMLYGYTFDGEFSPEEGYCYDSSKILLDPY 1921
            E+RVT++I+LDPL NKTGDVWHVFLKG+FEN++YGY FDG+FSPEEG+ YDSS++LLDPY
Sbjct: 118  EDRVTEQISLDPLTNKTGDVWHVFLKGNFENIVYGYKFDGKFSPEEGHYYDSSRLLLDPY 177

Query: 1920 AKAVISREEFGALGSEGNCWSQMAGMVPYNDNEFNWEGDLPLRHPQRDLIVYEMHVRGFT 1741
            AKAVISR EFG LG EGNCW  MAGM+P +D EF+WEGDLPL++PQ+DLI+YEMHVRGFT
Sbjct: 178  AKAVISRGEFGILGPEGNCWPLMAGMIPSSDAEFDWEGDLPLKYPQKDLIIYEMHVRGFT 237

Query: 1740 RHDSSETDFPGTYLGVVEKLDHLKELGVNCIELMPFHEYNELEYFSYNSILGNYKLNFWG 1561
            RH+SS T FPGTY GVVEKLDHLKELGVNCIELMP HE+NELEYFSYNS+L +Y++NFWG
Sbjct: 238  RHESSRTKFPGTYHGVVEKLDHLKELGVNCIELMPCHEFNELEYFSYNSVLDDYRVNFWG 297

Query: 1560 YSTVNFFSPMLRYSSAGIRNCGQDAINEVKILVREAHKRGIEVLMDVVFNHTAEGNEKGP 1381
            YSTVN+FSPM+RYSSAGI NCG DAINEVK+L+REAHKRGIEVLMDVVFNHTAEGNE GP
Sbjct: 298  YSTVNYFSPMIRYSSAGIHNCGHDAINEVKLLIREAHKRGIEVLMDVVFNHTAEGNENGP 357

Query: 1380 MLSFRGVDNSVYYMLAPKGEYYNYSGCGNTFNCNHPVVRQFIVDCLRYWVEEMHVDGFRF 1201
            +LSFRGVDNSVYYMLAPKGE+YNYSGCGNTFNCNHP+VRQFI+DCLRYWV EMHVDGFRF
Sbjct: 358  ILSFRGVDNSVYYMLAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWVTEMHVDGFRF 417

Query: 1200 DLASILTRSSSLWDAVNVYGNSTEGDMLTTGTPLGSPPLVEMISNDPILRHVKLIAEAWD 1021
            DLASI+TR SSLWDA+NVYGN  EGD LTTGTPL SPPL++MISNDPILR VKLIAEAWD
Sbjct: 418  DLASIMTRGSSLWDALNVYGNPAEGDSLTTGTPLSSPPLIDMISNDPILRGVKLIAEAWD 477

Query: 1020 TGGLYQVGVFPHWGIWSEWNGKYRDIVRQFIKGTGGLSGAFAECLCGSPNLYQ-GARKPW 844
             GGLYQVG+FPHWG+WSEWNGKYRDIVRQFIKG+ G SGAFAECLCGSPNLYQ G RKPW
Sbjct: 478  AGGLYQVGMFPHWGLWSEWNGKYRDIVRQFIKGSDGFSGAFAECLCGSPNLYQEGGRKPW 537

Query: 843  NSINFVCAHDGFTLADLVTYNDKHNLAXXXXXXXXXXXXNSWNCGQ 706
            NSINFVCAHDGFTLADLVTYN KHN A            NSWNCGQ
Sbjct: 538  NSINFVCAHDGFTLADLVTYNKKHNNANGEDNNDGENHNNSWNCGQ 583



 Score =  315 bits (806), Expect(2) = 0.0
 Identities = 139/181 (76%), Positives = 167/181 (92%)
 Frame = -3

Query: 716  IVVRGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKQESSSDFFRFCSLLTKFRRE 537
            +V +GVPMIYMGDEYGHTKGGNNNTYC DNY+NYFRWDKK+ES SDFFRFC L++KFR+E
Sbjct: 608  MVSQGVPMIYMGDEYGHTKGGNNNTYCQDNYMNYFRWDKKEESLSDFFRFCCLMSKFRQE 667

Query: 536  CESLGLDNFPTADRLQWHGHFPGVPDWSETSRFVAFSLIDSVKRELYIAFNTSHLPVIIT 357
            CESLGL++FPTA+RLQWHG  PG+PDWS+TSRFVAF+++DSVK E+Y+AFNTSHLP+IIT
Sbjct: 668  CESLGLNDFPTAERLQWHGRTPGMPDWSKTSRFVAFTMVDSVKGEIYVAFNTSHLPIIIT 727

Query: 356  LPKRPGYRWEPLVDTSKTSPFDFLSDDLPEKAIALEQYSHFLNADVYPMLSYSSIILLLT 177
            LP+RPGYRW+PLVDTSK +PFDFLS+D+PE+  A++QYS F  A++YPMLSYSSIILLL 
Sbjct: 728  LPERPGYRWQPLVDTSKPAPFDFLSNDVPERDTAVKQYSQFTEANLYPMLSYSSIILLLC 787

Query: 176  P 174
            P
Sbjct: 788  P 788


>emb|CBI40669.3| unnamed protein product [Vitis vinifera]
          Length = 809

 Score =  900 bits (2327), Expect(2) = 0.0
 Identities = 422/532 (79%), Positives = 468/532 (87%), Gaps = 7/532 (1%)
 Frame = -2

Query: 2280 TGEVAETVVIEKPESRRFQVFNGRPLPFGATVRDGGVNFAIHSSGAVSATLCLISLSTLQ 2101
            +G  AETVV+EKP+ + F VF G P P GAT RDGGVNFA++S  AVSATLCLIS S L+
Sbjct: 68   SGAEAETVVVEKPKLQPFLVFEGCPAPLGATARDGGVNFAVYSGNAVSATLCLISASDLE 127

Query: 2100 ENRVTKKINLDPLINKTGDVWHVFLKGDFENMLYGYTFDGEFSPEEGYCYDSSKILLDPY 1921
            E+RVT++I+LDPL NKTGDVWHVFLKG+FEN++YGY FDG+FSPEEG+ YDSS++LLDPY
Sbjct: 128  EDRVTEQISLDPLTNKTGDVWHVFLKGNFENIVYGYKFDGKFSPEEGHYYDSSRLLLDPY 187

Query: 1920 AKAVISREEFGALGSEGNCWSQMAGMVPYNDNEFNWEGDLPLRHPQRDLIVYEMHVRGFT 1741
            AKAVISR EFG LG EGNCW  MAGM+P +D EF+WEGDLPL++PQ+DLI+YEMHVRGFT
Sbjct: 188  AKAVISRGEFGILGPEGNCWPLMAGMIPSSDAEFDWEGDLPLKYPQKDLIIYEMHVRGFT 247

Query: 1740 RHDSSETDFPGTYLGVVEKLDHLKELGVNCIELMPFHEYNELEYFSYNSILGNYK----- 1576
            RH+SS T FPGTY GVVEKLDHLKELGVNCIELMP HE+NELEYFSYNS+L +Y+     
Sbjct: 248  RHESSRTKFPGTYHGVVEKLDHLKELGVNCIELMPCHEFNELEYFSYNSVLDDYRYTCIS 307

Query: 1575 -LNFWGYSTVNFFSPMLRYSSAGIRNCGQDAINEVKILVREAHKRGIEVLMDVVFNHTAE 1399
             +NFWGYSTVN+FSPM+RYSSAGI NCG DAINEVK+L+REAHKRGIEVLMDVVFNHTAE
Sbjct: 308  MVNFWGYSTVNYFSPMIRYSSAGIHNCGHDAINEVKLLIREAHKRGIEVLMDVVFNHTAE 367

Query: 1398 GNEKGPMLSFRGVDNSVYYMLAPKGEYYNYSGCGNTFNCNHPVVRQFIVDCLRYWVEEMH 1219
            GNE GP+LSFRGVDNSVYYMLAPKGE+YNYSGCGNTFNCNHP+VRQFI+DCLRYWV EMH
Sbjct: 368  GNENGPILSFRGVDNSVYYMLAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWVTEMH 427

Query: 1218 VDGFRFDLASILTRSSSLWDAVNVYGNSTEGDMLTTGTPLGSPPLVEMISNDPILRHVKL 1039
            VDGFRFDLASI+TR SSLWDA+NVYGN  EGD LTTGTPL SPPL++MISNDPILR VKL
Sbjct: 428  VDGFRFDLASIMTRGSSLWDALNVYGNPAEGDSLTTGTPLSSPPLIDMISNDPILRGVKL 487

Query: 1038 IAEAWDTGGLYQVGVFPHWGIWSEWNGKYRDIVRQFIKGTGGLSGAFAECLCGSPNLYQ- 862
            IAEAWD GGLYQVG+FPHWG+WSEWNGKYRDIVRQFIKG+ G SGAFAECLCGSPNLYQ 
Sbjct: 488  IAEAWDAGGLYQVGMFPHWGLWSEWNGKYRDIVRQFIKGSDGFSGAFAECLCGSPNLYQE 547

Query: 861  GARKPWNSINFVCAHDGFTLADLVTYNDKHNLAXXXXXXXXXXXXNSWNCGQ 706
            G RKPWNSINFVCAHDGFTLADLVTYN KHN A            NSWNCGQ
Sbjct: 548  GGRKPWNSINFVCAHDGFTLADLVTYNKKHNNANGEDNNDGENHNNSWNCGQ 599



 Score =  315 bits (806), Expect(2) = 0.0
 Identities = 139/181 (76%), Positives = 167/181 (92%)
 Frame = -3

Query: 716  IVVRGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKQESSSDFFRFCSLLTKFRRE 537
            +V +GVPMIYMGDEYGHTKGGNNNTYC DNY+NYFRWDKK+ES SDFFRFC L++KFR+E
Sbjct: 624  MVSQGVPMIYMGDEYGHTKGGNNNTYCQDNYMNYFRWDKKEESLSDFFRFCCLMSKFRQE 683

Query: 536  CESLGLDNFPTADRLQWHGHFPGVPDWSETSRFVAFSLIDSVKRELYIAFNTSHLPVIIT 357
            CESLGL++FPTA+RLQWHG  PG+PDWS+TSRFVAF+++DSVK E+Y+AFNTSHLP+IIT
Sbjct: 684  CESLGLNDFPTAERLQWHGRTPGMPDWSKTSRFVAFTMVDSVKGEIYVAFNTSHLPIIIT 743

Query: 356  LPKRPGYRWEPLVDTSKTSPFDFLSDDLPEKAIALEQYSHFLNADVYPMLSYSSIILLLT 177
            LP+RPGYRW+PLVDTSK +PFDFLS+D+PE+  A++QYS F  A++YPMLSYSSIILLL 
Sbjct: 744  LPERPGYRWQPLVDTSKPAPFDFLSNDVPERDTAVKQYSQFTEANLYPMLSYSSIILLLC 803

Query: 176  P 174
            P
Sbjct: 804  P 804


>ref|XP_012459147.1| PREDICTED: isoamylase 1, chloroplastic isoform X1 [Gossypium
            raimondii] gi|763807903|gb|KJB74805.1| hypothetical
            protein B456_012G008800 [Gossypium raimondii]
          Length = 789

 Score =  895 bits (2313), Expect(2) = 0.0
 Identities = 414/523 (79%), Positives = 469/523 (89%), Gaps = 2/523 (0%)
 Frame = -2

Query: 2268 AETVVIEKPES-RRFQVFNGRPLPFGATVRDGGVNFAIHSSGAVSATLCLISLSTLQENR 2092
            AET +++KP+  +RFQV +G P PFGAT++DGGVNFAI+S+ A SATLCLI+LS LQ+NR
Sbjct: 58   AETALVDKPKVLKRFQVSDGHPAPFGATIQDGGVNFAIYSANATSATLCLIALSDLQQNR 117

Query: 2091 VTKKINLDPLINKTGDVWHVFLKGDFENMLYGYTFDGEFSPEEGYCYDSSKILLDPYAKA 1912
            VT++I LDPL NKTGDVWHVFLKG+F++MLYGY FDGEFSPE G+ YDSS+ILLDPYAKA
Sbjct: 118  VTEQIPLDPLANKTGDVWHVFLKGNFKDMLYGYIFDGEFSPEIGHYYDSSRILLDPYAKA 177

Query: 1911 VISREEFGALGSEGNCWSQMAGMVPYNDNEFNWEGDLPLRHPQRDLIVYEMHVRGFTRHD 1732
            VISR EFGALG E NCW QMAGMVP ++++F+WEGDLPLRHP RDLI+YEMHVRG+TRH+
Sbjct: 178  VISRGEFGALGPEDNCWPQMAGMVPTSEDQFDWEGDLPLRHPGRDLIIYEMHVRGYTRHE 237

Query: 1731 SSETDFPGTYLGVVEKLDHLKELGVNCIELMPFHEYNELEYFSYNSILGNYKLNFWGYST 1552
            SS T FPGTY GVVEKLDHLKELGVNCIELMP HE+NELEY+SYNS+LG+YKLNFWGYST
Sbjct: 238  SSGTKFPGTYCGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYKLNFWGYST 297

Query: 1551 VNFFSPMLRYSSAGIRNCGQDAINEVKILVREAHKRGIEVLMDVVFNHTAEGNEKGPMLS 1372
            +N+FSPM+RYSS+GIR+CG+DAINE K LV+EAHKRGIEV+MDVVFNHTAEGNEKG  +S
Sbjct: 298  INYFSPMIRYSSSGIRSCGRDAINEFKYLVKEAHKRGIEVIMDVVFNHTAEGNEKGLSVS 357

Query: 1371 FRGVDNSVYYMLAPKGEYYNYSGCGNTFNCNHPVVRQFIVDCLRYWVEEMHVDGFRFDLA 1192
            FRGVDN VYYMLAPKGEYYNYSGCGNTFNCNHPVVRQFI+DCLRYWV EMHVDGFRFDLA
Sbjct: 358  FRGVDNCVYYMLAPKGEYYNYSGCGNTFNCNHPVVRQFILDCLRYWVAEMHVDGFRFDLA 417

Query: 1191 SILTRSSSLWDAVNVYGNSTEGDMLTTGTPLGSPPLVEMISNDPILRHVKLIAEAWDTGG 1012
            SI+TRSSSLWD+VNVYG+  EGD++TTGTPL +PPL++M+SNDP+L  VKLIAEAWD GG
Sbjct: 418  SIMTRSSSLWDSVNVYGDPLEGDLITTGTPLSNPPLIDMMSNDPVLHGVKLIAEAWDAGG 477

Query: 1011 LYQVGVFPHWGIWSEWNGKYRDIVRQFIKGTGGLSGAFAECLCGSPNLYQ-GARKPWNSI 835
            LYQVG FPHWG+WSEWNGKYRDIVRQFIKGT GLSGAFAECLCGSPNLYQ G  KPWNS+
Sbjct: 478  LYQVGRFPHWGVWSEWNGKYRDIVRQFIKGTDGLSGAFAECLCGSPNLYQKGGGKPWNSV 537

Query: 834  NFVCAHDGFTLADLVTYNDKHNLAXXXXXXXXXXXXNSWNCGQ 706
            NF+CAHDGFTLADLVTYN+KHNLA             SWNCGQ
Sbjct: 538  NFICAHDGFTLADLVTYNNKHNLANGEDNNDGESHNYSWNCGQ 580



 Score =  322 bits (824), Expect(2) = 0.0
 Identities = 143/182 (78%), Positives = 170/182 (93%)
 Frame = -3

Query: 716  IVVRGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKQESSSDFFRFCSLLTKFRRE 537
            +V +GVPMI+MGDEYGHTKGGNNNTYCHDNYINYFRWDKK+ES+SD FRF  L+T FRRE
Sbjct: 605  MVSQGVPMIHMGDEYGHTKGGNNNTYCHDNYINYFRWDKKEESASDLFRFFCLMTMFRRE 664

Query: 536  CESLGLDNFPTADRLQWHGHFPGVPDWSETSRFVAFSLIDSVKRELYIAFNTSHLPVIIT 357
            CESLGL++FPTA+RLQWHGH PG PDWS+TSRFVAF+LIDSVK E+Y+AFNTSHLPV IT
Sbjct: 665  CESLGLNDFPTAERLQWHGHTPGKPDWSDTSRFVAFTLIDSVKGEIYVAFNTSHLPVTIT 724

Query: 356  LPKRPGYRWEPLVDTSKTSPFDFLSDDLPEKAIALEQYSHFLNADVYPMLSYSSIILLLT 177
            LP RPGYRWEPLVDTSK +PFDFLS+++PE+ +A++QY+HFL+A++YPMLSYSSI+LLL+
Sbjct: 725  LPDRPGYRWEPLVDTSKPAPFDFLSNEVPERDLAIKQYAHFLDANLYPMLSYSSIVLLLS 784

Query: 176  PD 171
            PD
Sbjct: 785  PD 786


>ref|XP_006474309.1| PREDICTED: isoamylase 1, chloroplastic-like [Citrus sinensis]
          Length = 805

 Score =  895 bits (2312), Expect(2) = 0.0
 Identities = 414/521 (79%), Positives = 468/521 (89%), Gaps = 1/521 (0%)
 Frame = -2

Query: 2265 ETVVIEKPESRRFQVFNGRPLPFGATVRDGGVNFAIHSSGAVSATLCLISLSTLQENRVT 2086
            ET VIEKP S+RFQV  G P PFGAT+RDGGVNF+I SS AVSA+LCLI+LS LQEN+VT
Sbjct: 76   ETAVIEKPPSQRFQVSKGYPTPFGATLRDGGVNFSIFSSNAVSASLCLITLSDLQENKVT 135

Query: 2085 KKINLDPLINKTGDVWHVFLKGDFENMLYGYTFDGEFSPEEGYCYDSSKILLDPYAKAVI 1906
            ++I LD   NKTGDVWHVFLKGDF++MLYGY FDG+FSP+EG+ +D +KI+LDPYAKAVI
Sbjct: 136  EEIALDSFANKTGDVWHVFLKGDFKDMLYGYKFDGKFSPQEGHYFDPTKIVLDPYAKAVI 195

Query: 1905 SREEFGALGSEGNCWSQMAGMVPYNDNEFNWEGDLPLRHPQRDLIVYEMHVRGFTRHDSS 1726
            SR +FG LG + NCW QMA +VP  ++EF+WEGDLPL++PQRDLI+YE+HVRGFTRH+SS
Sbjct: 196  SRAQFGVLGPDENCWPQMACLVPTPEDEFDWEGDLPLKYPQRDLIIYEVHVRGFTRHESS 255

Query: 1725 ETDFPGTYLGVVEKLDHLKELGVNCIELMPFHEYNELEYFSYNSILGNYKLNFWGYSTVN 1546
            +T+ PGTYLGVVEKLDHLK+LG+NC+ELMP HE+NELEYFSYNS+LG+YK+NFWGYST+N
Sbjct: 256  KTEHPGTYLGVVEKLDHLKDLGINCLELMPCHEFNELEYFSYNSVLGDYKVNFWGYSTIN 315

Query: 1545 FFSPMLRYSSAGIRNCGQDAINEVKILVREAHKRGIEVLMDVVFNHTAEGNEKGPMLSFR 1366
            +FSPM+ YSSAGIRNCG DAINE K+LVREAHKRGIEV+MDVVFNHTAEGN+KGP+LSFR
Sbjct: 316  YFSPMISYSSAGIRNCGHDAINEFKLLVREAHKRGIEVVMDVVFNHTAEGNDKGPILSFR 375

Query: 1365 GVDNSVYYMLAPKGEYYNYSGCGNTFNCNHPVVRQFIVDCLRYWVEEMHVDGFRFDLASI 1186
            GVDNSVYYMLAPKGE+YNYSGCGNTFNCNHPVVRQFIVDCLRYWV EMHVDGFRFDLASI
Sbjct: 376  GVDNSVYYMLAPKGEFYNYSGCGNTFNCNHPVVRQFIVDCLRYWVTEMHVDGFRFDLASI 435

Query: 1185 LTRSSSLWDAVNVYGNSTEGDMLTTGTPLGSPPLVEMISNDPILRHVKLIAEAWDTGGLY 1006
            +TR SSLWD+VNVYG   EGD+LTTGTPL SPPL+++ISNDPILR VKLIAEAWDTGGLY
Sbjct: 436  MTRGSSLWDSVNVYGIPIEGDLLTTGTPLSSPPLIDLISNDPILRGVKLIAEAWDTGGLY 495

Query: 1005 QVGVFPHWGIWSEWNGKYRDIVRQFIKGTGGLSGAFAECLCGSPNLYQ-GARKPWNSINF 829
            QVG+FPHWGIWSEWNGKYRDIVRQFIKGT G +GAFAECLCGSPNLYQ G RKPWNSINF
Sbjct: 496  QVGIFPHWGIWSEWNGKYRDIVRQFIKGTDGFAGAFAECLCGSPNLYQGGGRKPWNSINF 555

Query: 828  VCAHDGFTLADLVTYNDKHNLAXXXXXXXXXXXXNSWNCGQ 706
            VCAHDGF+LADLV+YN KHNLA            NSWNCGQ
Sbjct: 556  VCAHDGFSLADLVSYNQKHNLANGEDNNDGETHNNSWNCGQ 596



 Score =  316 bits (809), Expect(2) = 0.0
 Identities = 145/182 (79%), Positives = 163/182 (89%)
 Frame = -3

Query: 716  IVVRGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKQESSSDFFRFCSLLTKFRRE 537
            +V +GVPMI MGDEYGHTKGGNNNTYCHDN INYFRWDKK+ES SDFFRFC LLTKFR E
Sbjct: 621  MVSQGVPMISMGDEYGHTKGGNNNTYCHDNDINYFRWDKKEESKSDFFRFCCLLTKFRHE 680

Query: 536  CESLGLDNFPTADRLQWHGHFPGVPDWSETSRFVAFSLIDSVKRELYIAFNTSHLPVIIT 357
            CESLGL +FPTADRLQWHGH PG+PDWS+ SRFVAF+LIDSVK ++Y+AFN SHLPVII+
Sbjct: 681  CESLGLSDFPTADRLQWHGHAPGLPDWSDKSRFVAFTLIDSVKGKIYVAFNASHLPVIIS 740

Query: 356  LPKRPGYRWEPLVDTSKTSPFDFLSDDLPEKAIALEQYSHFLNADVYPMLSYSSIILLLT 177
            LP+RPGYRWEPLVDTSK  PFDFLS DLP K IA++QY+ FL+A++YPMLSYSSIILL  
Sbjct: 741  LPERPGYRWEPLVDTSKPVPFDFLSSDLPAKEIAIKQYAPFLDANLYPMLSYSSIILLQL 800

Query: 176  PD 171
            PD
Sbjct: 801  PD 802


>gb|KDO61988.1| hypothetical protein CISIN_1g003653mg [Citrus sinensis]
          Length = 805

 Score =  894 bits (2309), Expect(2) = 0.0
 Identities = 413/521 (79%), Positives = 468/521 (89%), Gaps = 1/521 (0%)
 Frame = -2

Query: 2265 ETVVIEKPESRRFQVFNGRPLPFGATVRDGGVNFAIHSSGAVSATLCLISLSTLQENRVT 2086
            ET VI+KP+S+RFQV  G P PFGAT+RDGGVNF+I SS AVSATLCLI+LS LQEN+VT
Sbjct: 76   ETAVIKKPQSQRFQVSKGYPTPFGATLRDGGVNFSIFSSNAVSATLCLITLSDLQENKVT 135

Query: 2085 KKINLDPLINKTGDVWHVFLKGDFENMLYGYTFDGEFSPEEGYCYDSSKILLDPYAKAVI 1906
            ++I LD   NKTGDVWHVFLKGDF++MLYGY FDG+FSP+EG+ +D +KI+LDPYAKAVI
Sbjct: 136  EEIALDSFANKTGDVWHVFLKGDFKDMLYGYKFDGKFSPQEGHYFDPTKIVLDPYAKAVI 195

Query: 1905 SREEFGALGSEGNCWSQMAGMVPYNDNEFNWEGDLPLRHPQRDLIVYEMHVRGFTRHDSS 1726
            SR +FG LG + NCW QMA +VP  ++EF+WEGDLPL++PQRDLI+YE+HVRGFTRH+SS
Sbjct: 196  SRAQFGVLGPDENCWPQMACLVPTPEDEFDWEGDLPLKYPQRDLIIYEVHVRGFTRHESS 255

Query: 1725 ETDFPGTYLGVVEKLDHLKELGVNCIELMPFHEYNELEYFSYNSILGNYKLNFWGYSTVN 1546
            +T+ PGTYLGVVEKLDHLK+LG+NC+ELMP HE+NELEYFSYNS+LG+YK+NFWGYST+N
Sbjct: 256  KTEHPGTYLGVVEKLDHLKDLGINCLELMPCHEFNELEYFSYNSVLGDYKVNFWGYSTIN 315

Query: 1545 FFSPMLRYSSAGIRNCGQDAINEVKILVREAHKRGIEVLMDVVFNHTAEGNEKGPMLSFR 1366
            +FSPM+ YSSAGIRNCG DAINE K+LVREAHKRGIEV+MDVVFNHT EGN+KGP+LSFR
Sbjct: 316  YFSPMISYSSAGIRNCGHDAINEFKLLVREAHKRGIEVVMDVVFNHTVEGNDKGPILSFR 375

Query: 1365 GVDNSVYYMLAPKGEYYNYSGCGNTFNCNHPVVRQFIVDCLRYWVEEMHVDGFRFDLASI 1186
            GVDNSVYYMLAPKGE+YNYSGCGNTFNCNHPVVRQFIVDCLRYWV EMHVDGFRFDLASI
Sbjct: 376  GVDNSVYYMLAPKGEFYNYSGCGNTFNCNHPVVRQFIVDCLRYWVTEMHVDGFRFDLASI 435

Query: 1185 LTRSSSLWDAVNVYGNSTEGDMLTTGTPLGSPPLVEMISNDPILRHVKLIAEAWDTGGLY 1006
            +TR SSLWD+VNVYG   EGD+LTTGTPL SPPL+++ISNDPILR VKLIAEAWDTGGLY
Sbjct: 436  MTRGSSLWDSVNVYGIPIEGDLLTTGTPLRSPPLIDLISNDPILRGVKLIAEAWDTGGLY 495

Query: 1005 QVGVFPHWGIWSEWNGKYRDIVRQFIKGTGGLSGAFAECLCGSPNLYQ-GARKPWNSINF 829
            QVG+FPHWGIWSEWNGKYRDIVRQFIKGT G +GAFAECLCGSPNLYQ G RKPWNSINF
Sbjct: 496  QVGIFPHWGIWSEWNGKYRDIVRQFIKGTDGFAGAFAECLCGSPNLYQGGGRKPWNSINF 555

Query: 828  VCAHDGFTLADLVTYNDKHNLAXXXXXXXXXXXXNSWNCGQ 706
            VCAHDGF+LADLV+YN KHNLA            NSWNCGQ
Sbjct: 556  VCAHDGFSLADLVSYNQKHNLANGEDNNDGETHNNSWNCGQ 596



 Score =  323 bits (827), Expect(2) = 0.0
 Identities = 148/182 (81%), Positives = 165/182 (90%)
 Frame = -3

Query: 716  IVVRGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKQESSSDFFRFCSLLTKFRRE 537
            +V +GVPMI MGDEYGHTKGGNNNTYCHDN INYFRWDKK+ES SDFFRFC LLTKFR E
Sbjct: 621  MVSQGVPMISMGDEYGHTKGGNNNTYCHDNDINYFRWDKKEESKSDFFRFCCLLTKFRHE 680

Query: 536  CESLGLDNFPTADRLQWHGHFPGVPDWSETSRFVAFSLIDSVKRELYIAFNTSHLPVIIT 357
            CESLGL +FPTADRLQWHGH PG+PDWS+ SRFVAF+LIDSVK E+Y+AFN SHLPVII+
Sbjct: 681  CESLGLSDFPTADRLQWHGHAPGLPDWSDKSRFVAFTLIDSVKGEIYVAFNASHLPVIIS 740

Query: 356  LPKRPGYRWEPLVDTSKTSPFDFLSDDLPEKAIALEQYSHFLNADVYPMLSYSSIILLLT 177
            LPKRPGYRWEPLVDTSK  PFDFLS DLP K IA++QY+ FL+A++YPMLSYSSIILLL+
Sbjct: 741  LPKRPGYRWEPLVDTSKPEPFDFLSSDLPAKEIAIKQYAPFLDANLYPMLSYSSIILLLS 800

Query: 176  PD 171
            PD
Sbjct: 801  PD 802


>ref|XP_011020102.1| PREDICTED: isoamylase 1, chloroplastic-like isoform X2 [Populus
            euphratica]
          Length = 839

 Score =  892 bits (2304), Expect(2) = 0.0
 Identities = 418/533 (78%), Positives = 466/533 (87%), Gaps = 5/533 (0%)
 Frame = -2

Query: 2289 VNSTGEVAETVVI----EKPESRRFQVFNGRPLPFGATVRDGGVNFAIHSSGAVSATLCL 2122
            + +  E  +T V+    E+P+ ++ QVF G P PFGATVRDGGVNFAI S+ AVSATLCL
Sbjct: 98   IKAASEGVDTAVVVVEEEEPQLKKIQVFEGHPAPFGATVRDGGVNFAIFSANAVSATLCL 157

Query: 2121 ISLSTLQENRVTKKINLDPLINKTGDVWHVFLKGDFENMLYGYTFDGEFSPEEGYCYDSS 1942
            ISLS L ENRVT++I LDPL NKTGDVWHV LKGDF++MLYGY FDG FSPE G  YD S
Sbjct: 158  ISLSDLPENRVTEQIFLDPLTNKTGDVWHVLLKGDFKDMLYGYKFDGNFSPEVGLYYDPS 217

Query: 1941 KILLDPYAKAVISREEFGALGSEGNCWSQMAGMVPYNDNEFNWEGDLPLRHPQRDLIVYE 1762
            KI+LDPYAK+VISR EFG LG + N W QMA M+P  +N+F+WEGD PL++PQRDLI+YE
Sbjct: 218  KIVLDPYAKSVISRGEFGVLGHDDNRWPQMACMIPTAENKFDWEGDSPLKYPQRDLIIYE 277

Query: 1761 MHVRGFTRHDSSETDFPGTYLGVVEKLDHLKELGVNCIELMPFHEYNELEYFSYNSILGN 1582
            MHVRGFT+H+SS T+FPGTYLGVVEKLDHLKELGVNCIELMP HE+NELEY+SYNS+LG+
Sbjct: 278  MHVRGFTQHESSRTEFPGTYLGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGD 337

Query: 1581 YKLNFWGYSTVNFFSPMLRYSSAGIRNCGQDAINEVKILVREAHKRGIEVLMDVVFNHTA 1402
            YK+NFWGYST+N+FSPM RYSSAG RNCGQDAINE K+LVREAHKRGIEV MDVVFNHTA
Sbjct: 338  YKVNFWGYSTINYFSPMTRYSSAGTRNCGQDAINEFKLLVREAHKRGIEVFMDVVFNHTA 397

Query: 1401 EGNEKGPMLSFRGVDNSVYYMLAPKGEYYNYSGCGNTFNCNHPVVRQFIVDCLRYWVEEM 1222
            EGNEKGP+LSFRGVDNS+YYMLAPKGE+YNYSGCGNTFNCNHP+VRQFI+DCLRYWV EM
Sbjct: 398  EGNEKGPILSFRGVDNSIYYMLAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWVTEM 457

Query: 1221 HVDGFRFDLASILTRSSSLWDAVNVYGNSTEGDMLTTGTPLGSPPLVEMISNDPILRHVK 1042
            HVDGFRFDLASI+TRSSSLWDAVNV+G+  EGD+LTTGTPL SPPL++M+SNDPILR VK
Sbjct: 458  HVDGFRFDLASIMTRSSSLWDAVNVFGSPIEGDLLTTGTPLSSPPLIDMMSNDPILRDVK 517

Query: 1041 LIAEAWDTGGLYQVGVFPHWGIWSEWNGKYRDIVRQFIKGTGGLSGAFAECLCGSPNLYQ 862
            LIAEAWD GGLYQVG+FPHW IWSEWNGKYRDIVRQFIKGT G SGAFAECLCGSPNLYQ
Sbjct: 518  LIAEAWDAGGLYQVGMFPHWCIWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQ 577

Query: 861  -GARKPWNSINFVCAHDGFTLADLVTYNDKHNLAXXXXXXXXXXXXNSWNCGQ 706
             G RKPWNSINFVCAHDGFTLADLVTYN+KHNLA            NSWNCGQ
Sbjct: 578  EGGRKPWNSINFVCAHDGFTLADLVTYNEKHNLANGEDNNDGENHNNSWNCGQ 630



 Score =  327 bits (837), Expect(2) = 0.0
 Identities = 146/184 (79%), Positives = 170/184 (92%)
 Frame = -3

Query: 716  IVVRGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKQESSSDFFRFCSLLTKFRRE 537
            +V +GVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKK+ESSSDFFRFC L+TKFR E
Sbjct: 655  MVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKEESSSDFFRFCRLVTKFRHE 714

Query: 536  CESLGLDNFPTADRLQWHGHFPGVPDWSETSRFVAFSLIDSVKRELYIAFNTSHLPVIIT 357
            CESLGL++FPTA+RLQWHGH PG PDWSETSRFVAF+LIDSVK E+YIAFN SHL V IT
Sbjct: 715  CESLGLNDFPTAERLQWHGHAPGTPDWSETSRFVAFTLIDSVKGEIYIAFNASHLAVTIT 774

Query: 356  LPKRPGYRWEPLVDTSKTSPFDFLSDDLPEKAIALEQYSHFLNADVYPMLSYSSIILLLT 177
            LP+RPGYRWEPLVD+ K +PFDFLS D+PE+ +A++QYSHFL A++YPMLSY+SIIL+L+
Sbjct: 775  LPERPGYRWEPLVDSGKPAPFDFLSSDVPERDLAVKQYSHFLEANLYPMLSYTSIILVLS 834

Query: 176  PDGD 165
            P+G+
Sbjct: 835  PNGN 838


>ref|XP_011020101.1| PREDICTED: isoamylase 1, chloroplastic-like isoform X1 [Populus
            euphratica]
          Length = 870

 Score =  892 bits (2304), Expect(2) = 0.0
 Identities = 418/533 (78%), Positives = 466/533 (87%), Gaps = 5/533 (0%)
 Frame = -2

Query: 2289 VNSTGEVAETVVI----EKPESRRFQVFNGRPLPFGATVRDGGVNFAIHSSGAVSATLCL 2122
            + +  E  +T V+    E+P+ ++ QVF G P PFGATVRDGGVNFAI S+ AVSATLCL
Sbjct: 98   IKAASEGVDTAVVVVEEEEPQLKKIQVFEGHPAPFGATVRDGGVNFAIFSANAVSATLCL 157

Query: 2121 ISLSTLQENRVTKKINLDPLINKTGDVWHVFLKGDFENMLYGYTFDGEFSPEEGYCYDSS 1942
            ISLS L ENRVT++I LDPL NKTGDVWHV LKGDF++MLYGY FDG FSPE G  YD S
Sbjct: 158  ISLSDLPENRVTEQIFLDPLTNKTGDVWHVLLKGDFKDMLYGYKFDGNFSPEVGLYYDPS 217

Query: 1941 KILLDPYAKAVISREEFGALGSEGNCWSQMAGMVPYNDNEFNWEGDLPLRHPQRDLIVYE 1762
            KI+LDPYAK+VISR EFG LG + N W QMA M+P  +N+F+WEGD PL++PQRDLI+YE
Sbjct: 218  KIVLDPYAKSVISRGEFGVLGHDDNRWPQMACMIPTAENKFDWEGDSPLKYPQRDLIIYE 277

Query: 1761 MHVRGFTRHDSSETDFPGTYLGVVEKLDHLKELGVNCIELMPFHEYNELEYFSYNSILGN 1582
            MHVRGFT+H+SS T+FPGTYLGVVEKLDHLKELGVNCIELMP HE+NELEY+SYNS+LG+
Sbjct: 278  MHVRGFTQHESSRTEFPGTYLGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGD 337

Query: 1581 YKLNFWGYSTVNFFSPMLRYSSAGIRNCGQDAINEVKILVREAHKRGIEVLMDVVFNHTA 1402
            YK+NFWGYST+N+FSPM RYSSAG RNCGQDAINE K+LVREAHKRGIEV MDVVFNHTA
Sbjct: 338  YKVNFWGYSTINYFSPMTRYSSAGTRNCGQDAINEFKLLVREAHKRGIEVFMDVVFNHTA 397

Query: 1401 EGNEKGPMLSFRGVDNSVYYMLAPKGEYYNYSGCGNTFNCNHPVVRQFIVDCLRYWVEEM 1222
            EGNEKGP+LSFRGVDNS+YYMLAPKGE+YNYSGCGNTFNCNHP+VRQFI+DCLRYWV EM
Sbjct: 398  EGNEKGPILSFRGVDNSIYYMLAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWVTEM 457

Query: 1221 HVDGFRFDLASILTRSSSLWDAVNVYGNSTEGDMLTTGTPLGSPPLVEMISNDPILRHVK 1042
            HVDGFRFDLASI+TRSSSLWDAVNV+G+  EGD+LTTGTPL SPPL++M+SNDPILR VK
Sbjct: 458  HVDGFRFDLASIMTRSSSLWDAVNVFGSPIEGDLLTTGTPLSSPPLIDMMSNDPILRDVK 517

Query: 1041 LIAEAWDTGGLYQVGVFPHWGIWSEWNGKYRDIVRQFIKGTGGLSGAFAECLCGSPNLYQ 862
            LIAEAWD GGLYQVG+FPHW IWSEWNGKYRDIVRQFIKGT G SGAFAECLCGSPNLYQ
Sbjct: 518  LIAEAWDAGGLYQVGMFPHWCIWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQ 577

Query: 861  -GARKPWNSINFVCAHDGFTLADLVTYNDKHNLAXXXXXXXXXXXXNSWNCGQ 706
             G RKPWNSINFVCAHDGFTLADLVTYN+KHNLA            NSWNCGQ
Sbjct: 578  EGGRKPWNSINFVCAHDGFTLADLVTYNEKHNLANGEDNNDGENHNNSWNCGQ 630



 Score =  310 bits (795), Expect(2) = 0.0
 Identities = 146/215 (67%), Positives = 170/215 (79%), Gaps = 31/215 (14%)
 Frame = -3

Query: 716  IVVRGVPMIYMGDEYGHTKGGNNNTYCHDNY----------------------------- 624
            +V +GVPMIYMGDEYGHTKGGNNNTYCHDNY                             
Sbjct: 655  MVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYVTFYTQPLLRCSYPLSKLTVDYVHYLSTT 714

Query: 623  --INYFRWDKKQESSSDFFRFCSLLTKFRRECESLGLDNFPTADRLQWHGHFPGVPDWSE 450
              INYFRWDKK+ESSSDFFRFC L+TKFR ECESLGL++FPTA+RLQWHGH PG PDWSE
Sbjct: 715  MQINYFRWDKKEESSSDFFRFCRLVTKFRHECESLGLNDFPTAERLQWHGHAPGTPDWSE 774

Query: 449  TSRFVAFSLIDSVKRELYIAFNTSHLPVIITLPKRPGYRWEPLVDTSKTSPFDFLSDDLP 270
            TSRFVAF+LIDSVK E+YIAFN SHL V ITLP+RPGYRWEPLVD+ K +PFDFLS D+P
Sbjct: 775  TSRFVAFTLIDSVKGEIYIAFNASHLAVTITLPERPGYRWEPLVDSGKPAPFDFLSSDVP 834

Query: 269  EKAIALEQYSHFLNADVYPMLSYSSIILLLTPDGD 165
            E+ +A++QYSHFL A++YPMLSY+SIIL+L+P+G+
Sbjct: 835  ERDLAVKQYSHFLEANLYPMLSYTSIILVLSPNGN 869


>ref|XP_002308090.2| isoamylase family protein [Populus trichocarpa]
            gi|550335674|gb|EEE91613.2| isoamylase family protein
            [Populus trichocarpa]
          Length = 876

 Score =  891 bits (2303), Expect(2) = 0.0
 Identities = 419/533 (78%), Positives = 465/533 (87%), Gaps = 5/533 (0%)
 Frame = -2

Query: 2289 VNSTGEVAETVVI----EKPESRRFQVFNGRPLPFGATVRDGGVNFAIHSSGAVSATLCL 2122
            + +  E  +T V+    E+P+ ++ QVF G P PFGATVRDGGVNFAI S+ AVSATLCL
Sbjct: 104  IKAASEGVDTAVVVVEEEEPKLKKIQVFEGHPAPFGATVRDGGVNFAIFSADAVSATLCL 163

Query: 2121 ISLSTLQENRVTKKINLDPLINKTGDVWHVFLKGDFENMLYGYTFDGEFSPEEGYCYDSS 1942
            ISLS L ENRVT++I LDPL NKTGDVWHV LKGDF++MLYGY FDG FSPE G  YD S
Sbjct: 164  ISLSDLPENRVTEQIFLDPLTNKTGDVWHVLLKGDFKDMLYGYKFDGNFSPEVGLYYDPS 223

Query: 1941 KILLDPYAKAVISREEFGALGSEGNCWSQMAGMVPYNDNEFNWEGDLPLRHPQRDLIVYE 1762
            KI+LDPYAK+VISR EFG LG + N W QMA M+P  +N+F+WEGD PL+HPQRDLI+YE
Sbjct: 224  KIVLDPYAKSVISRGEFGVLGHDDNRWPQMACMIPTAENKFDWEGDSPLKHPQRDLIIYE 283

Query: 1761 MHVRGFTRHDSSETDFPGTYLGVVEKLDHLKELGVNCIELMPFHEYNELEYFSYNSILGN 1582
            MHVRGFT+H+SS T+FPGTYLGVVEKLDHLKELGVNCIELMP HE+NELEY+SYNS+LG+
Sbjct: 284  MHVRGFTQHESSRTEFPGTYLGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGD 343

Query: 1581 YKLNFWGYSTVNFFSPMLRYSSAGIRNCGQDAINEVKILVREAHKRGIEVLMDVVFNHTA 1402
            YK+NFWGYSTVN+FSPM RYSSAG RNCG+DAINE K+LVREAHKRGIEV MDVVFNHTA
Sbjct: 344  YKVNFWGYSTVNYFSPMTRYSSAGTRNCGRDAINEFKLLVREAHKRGIEVFMDVVFNHTA 403

Query: 1401 EGNEKGPMLSFRGVDNSVYYMLAPKGEYYNYSGCGNTFNCNHPVVRQFIVDCLRYWVEEM 1222
            EGNEKGP+LSFRGVDNS+YYMLAPKGE+YNYSGCGNTFNCNHP+VRQFI+DCLRYWV EM
Sbjct: 404  EGNEKGPILSFRGVDNSIYYMLAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWVTEM 463

Query: 1221 HVDGFRFDLASILTRSSSLWDAVNVYGNSTEGDMLTTGTPLGSPPLVEMISNDPILRHVK 1042
            HVDGFRFDLASI+TRSSSLWDAVNV+G+  EGD+LTTGTPL SPPL++M+SNDPILR VK
Sbjct: 464  HVDGFRFDLASIMTRSSSLWDAVNVFGSPIEGDLLTTGTPLSSPPLIDMMSNDPILRDVK 523

Query: 1041 LIAEAWDTGGLYQVGVFPHWGIWSEWNGKYRDIVRQFIKGTGGLSGAFAECLCGSPNLYQ 862
            LIAEAWD GGLYQVG+FPHW IWSEWNGKYRDIVRQFIKGT G SGAFAECLCGSPNLYQ
Sbjct: 524  LIAEAWDAGGLYQVGMFPHWRIWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQ 583

Query: 861  -GARKPWNSINFVCAHDGFTLADLVTYNDKHNLAXXXXXXXXXXXXNSWNCGQ 706
             G RKPWNSINFVCAHDGFTLADLVTYN KHNLA            NSWNCGQ
Sbjct: 584  EGGRKPWNSINFVCAHDGFTLADLVTYNKKHNLANGEDNNDGENHNNSWNCGQ 636



 Score =  306 bits (783), Expect(2) = 0.0
 Identities = 144/213 (67%), Positives = 167/213 (78%), Gaps = 31/213 (14%)
 Frame = -3

Query: 716  IVVRGVPMIYMGDEYGHTKGGNNNTYCHDNY----------------------------- 624
            +V +GVPMIYMGDEYGHTKGGNNNTYCHDNY                             
Sbjct: 661  MVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYVTFYTQPLLRCSYPQSKLTVDYVHYLSTT 720

Query: 623  --INYFRWDKKQESSSDFFRFCSLLTKFRRECESLGLDNFPTADRLQWHGHFPGVPDWSE 450
              INYFRWDKK+ESSSDFFRFC L+TKFR ECESLGL++FP A+RLQWHGH PG PDWSE
Sbjct: 721  MQINYFRWDKKEESSSDFFRFCRLVTKFRHECESLGLNDFPKAERLQWHGHDPGTPDWSE 780

Query: 449  TSRFVAFSLIDSVKRELYIAFNTSHLPVIITLPKRPGYRWEPLVDTSKTSPFDFLSDDLP 270
            TSRFVAF+LIDSVK E+YIAFN SHL V ITLP+RPGYRWEPLVD+ K +PFDFLS D+P
Sbjct: 781  TSRFVAFTLIDSVKGEIYIAFNASHLAVTITLPERPGYRWEPLVDSGKPAPFDFLSSDIP 840

Query: 269  EKAIALEQYSHFLNADVYPMLSYSSIILLLTPD 171
            E+ +A++QYSHFL A++YPMLSY+SIIL+L+P+
Sbjct: 841  ERDLAIKQYSHFLEANLYPMLSYTSIILVLSPN 873


>ref|XP_006381137.1| hypothetical protein POPTR_0006s07010g [Populus trichocarpa]
            gi|550335673|gb|ERP58934.1| hypothetical protein
            POPTR_0006s07010g [Populus trichocarpa]
          Length = 845

 Score =  891 bits (2303), Expect(2) = 0.0
 Identities = 419/533 (78%), Positives = 465/533 (87%), Gaps = 5/533 (0%)
 Frame = -2

Query: 2289 VNSTGEVAETVVI----EKPESRRFQVFNGRPLPFGATVRDGGVNFAIHSSGAVSATLCL 2122
            + +  E  +T V+    E+P+ ++ QVF G P PFGATVRDGGVNFAI S+ AVSATLCL
Sbjct: 104  IKAASEGVDTAVVVVEEEEPKLKKIQVFEGHPAPFGATVRDGGVNFAIFSADAVSATLCL 163

Query: 2121 ISLSTLQENRVTKKINLDPLINKTGDVWHVFLKGDFENMLYGYTFDGEFSPEEGYCYDSS 1942
            ISLS L ENRVT++I LDPL NKTGDVWHV LKGDF++MLYGY FDG FSPE G  YD S
Sbjct: 164  ISLSDLPENRVTEQIFLDPLTNKTGDVWHVLLKGDFKDMLYGYKFDGNFSPEVGLYYDPS 223

Query: 1941 KILLDPYAKAVISREEFGALGSEGNCWSQMAGMVPYNDNEFNWEGDLPLRHPQRDLIVYE 1762
            KI+LDPYAK+VISR EFG LG + N W QMA M+P  +N+F+WEGD PL+HPQRDLI+YE
Sbjct: 224  KIVLDPYAKSVISRGEFGVLGHDDNRWPQMACMIPTAENKFDWEGDSPLKHPQRDLIIYE 283

Query: 1761 MHVRGFTRHDSSETDFPGTYLGVVEKLDHLKELGVNCIELMPFHEYNELEYFSYNSILGN 1582
            MHVRGFT+H+SS T+FPGTYLGVVEKLDHLKELGVNCIELMP HE+NELEY+SYNS+LG+
Sbjct: 284  MHVRGFTQHESSRTEFPGTYLGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGD 343

Query: 1581 YKLNFWGYSTVNFFSPMLRYSSAGIRNCGQDAINEVKILVREAHKRGIEVLMDVVFNHTA 1402
            YK+NFWGYSTVN+FSPM RYSSAG RNCG+DAINE K+LVREAHKRGIEV MDVVFNHTA
Sbjct: 344  YKVNFWGYSTVNYFSPMTRYSSAGTRNCGRDAINEFKLLVREAHKRGIEVFMDVVFNHTA 403

Query: 1401 EGNEKGPMLSFRGVDNSVYYMLAPKGEYYNYSGCGNTFNCNHPVVRQFIVDCLRYWVEEM 1222
            EGNEKGP+LSFRGVDNS+YYMLAPKGE+YNYSGCGNTFNCNHP+VRQFI+DCLRYWV EM
Sbjct: 404  EGNEKGPILSFRGVDNSIYYMLAPKGEFYNYSGCGNTFNCNHPIVRQFILDCLRYWVTEM 463

Query: 1221 HVDGFRFDLASILTRSSSLWDAVNVYGNSTEGDMLTTGTPLGSPPLVEMISNDPILRHVK 1042
            HVDGFRFDLASI+TRSSSLWDAVNV+G+  EGD+LTTGTPL SPPL++M+SNDPILR VK
Sbjct: 464  HVDGFRFDLASIMTRSSSLWDAVNVFGSPIEGDLLTTGTPLSSPPLIDMMSNDPILRDVK 523

Query: 1041 LIAEAWDTGGLYQVGVFPHWGIWSEWNGKYRDIVRQFIKGTGGLSGAFAECLCGSPNLYQ 862
            LIAEAWD GGLYQVG+FPHW IWSEWNGKYRDIVRQFIKGT G SGAFAECLCGSPNLYQ
Sbjct: 524  LIAEAWDAGGLYQVGMFPHWRIWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQ 583

Query: 861  -GARKPWNSINFVCAHDGFTLADLVTYNDKHNLAXXXXXXXXXXXXNSWNCGQ 706
             G RKPWNSINFVCAHDGFTLADLVTYN KHNLA            NSWNCGQ
Sbjct: 584  EGGRKPWNSINFVCAHDGFTLADLVTYNKKHNLANGEDNNDGENHNNSWNCGQ 636



 Score =  322 bits (825), Expect(2) = 0.0
 Identities = 144/182 (79%), Positives = 167/182 (91%)
 Frame = -3

Query: 716  IVVRGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKQESSSDFFRFCSLLTKFRRE 537
            +V +GVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKK+ESSSDFFRFC L+TKFR E
Sbjct: 661  MVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKEESSSDFFRFCRLVTKFRHE 720

Query: 536  CESLGLDNFPTADRLQWHGHFPGVPDWSETSRFVAFSLIDSVKRELYIAFNTSHLPVIIT 357
            CESLGL++FP A+RLQWHGH PG PDWSETSRFVAF+LIDSVK E+YIAFN SHL V IT
Sbjct: 721  CESLGLNDFPKAERLQWHGHDPGTPDWSETSRFVAFTLIDSVKGEIYIAFNASHLAVTIT 780

Query: 356  LPKRPGYRWEPLVDTSKTSPFDFLSDDLPEKAIALEQYSHFLNADVYPMLSYSSIILLLT 177
            LP+RPGYRWEPLVD+ K +PFDFLS D+PE+ +A++QYSHFL A++YPMLSY+SIIL+L+
Sbjct: 781  LPERPGYRWEPLVDSGKPAPFDFLSSDIPERDLAIKQYSHFLEANLYPMLSYTSIILVLS 840

Query: 176  PD 171
            P+
Sbjct: 841  PN 842


>ref|XP_008223817.1| PREDICTED: isoamylase 1, chloroplastic [Prunus mume]
          Length = 805

 Score =  886 bits (2290), Expect(2) = 0.0
 Identities = 407/524 (77%), Positives = 463/524 (88%), Gaps = 1/524 (0%)
 Frame = -2

Query: 2274 EVAETVVIEKPESRRFQVFNGRPLPFGATVRDGGVNFAIHSSGAVSATLCLISLSTLQEN 2095
            +V ET V+++P+ RRFQV  G P PFGAT  DGGVNFA++S+ AVS TLCLI+LS L+E+
Sbjct: 74   DVVETAVVDRPKLRRFQVSQGHPSPFGATTCDGGVNFAVYSANAVSVTLCLITLSDLEED 133

Query: 2094 RVTKKINLDPLINKTGDVWHVFLKGDFENMLYGYTFDGEFSPEEGYCYDSSKILLDPYAK 1915
            +VT++++LDPL NKTG+VWHVFLKGDF++ LYGY F+G+FSPEEG  YDSSKI+LDPYAK
Sbjct: 134  KVTEQLSLDPLTNKTGNVWHVFLKGDFKDTLYGYKFEGKFSPEEGLYYDSSKIVLDPYAK 193

Query: 1914 AVISREEFGALGSEGNCWSQMAGMVPYNDNEFNWEGDLPLRHPQRDLIVYEMHVRGFTRH 1735
            AVI R E+G L  +GNCW QMAG VP  D+EF+WEGDLPL++PQ+DLI+YE+HVRGFTRH
Sbjct: 194  AVIRRGEYGKLSPDGNCWPQMAGTVPSFDDEFDWEGDLPLKYPQKDLIIYEIHVRGFTRH 253

Query: 1734 DSSETDFPGTYLGVVEKLDHLKELGVNCIELMPFHEYNELEYFSYNSILGNYKLNFWGYS 1555
            +SS  + PGTYLG+VEKLDHLKELG+NCIELMP HE+NELEYF YNS+LG+YK+NFWGYS
Sbjct: 254  ESSMAELPGTYLGLVEKLDHLKELGINCIELMPCHEFNELEYFGYNSVLGDYKVNFWGYS 313

Query: 1554 TVNFFSPMLRYSSAGIRNCGQDAINEVKILVREAHKRGIEVLMDVVFNHTAEGNEKGPML 1375
            TVN+FSPM+RYSSAGIRNCG DAINEVK L++EAHKRGIEV+MDVVFNHTAEGNE GP+L
Sbjct: 314  TVNYFSPMIRYSSAGIRNCGHDAINEVKFLIKEAHKRGIEVIMDVVFNHTAEGNENGPIL 373

Query: 1374 SFRGVDNSVYYMLAPKGEYYNYSGCGNTFNCNHPVVRQFIVDCLRYWVEEMHVDGFRFDL 1195
            SFRG DNS+YYMLAPKGE+YNYSGCGNTFNCNHPVVRQFI+DCLRYWV EMHVDGFRFDL
Sbjct: 374  SFRGADNSIYYMLAPKGEFYNYSGCGNTFNCNHPVVRQFIIDCLRYWVTEMHVDGFRFDL 433

Query: 1194 ASILTRSSSLWDAVNVYGNSTEGDMLTTGTPLGSPPLVEMISNDPILRHVKLIAEAWDTG 1015
            ASI+TR SSLWDA NVYG   +GD+LTTGTPL +PPLV+MISNDPILR VKLIAEAWDTG
Sbjct: 434  ASIMTRGSSLWDATNVYGIPIKGDLLTTGTPLANPPLVDMISNDPILRGVKLIAEAWDTG 493

Query: 1014 GLYQVGVFPHWGIWSEWNGKYRDIVRQFIKGTGGLSGAFAECLCGSPNLYQ-GARKPWNS 838
            GLYQVG+FPHWGIWSEWNGKYRD VRQFIKGT G SGA AECLCGSPNLYQ G R+PWNS
Sbjct: 494  GLYQVGMFPHWGIWSEWNGKYRDTVRQFIKGTDGFSGALAECLCGSPNLYQEGGRRPWNS 553

Query: 837  INFVCAHDGFTLADLVTYNDKHNLAXXXXXXXXXXXXNSWNCGQ 706
            INFVCAHDGFTLADLVTYN+KHNLA            NSWNCGQ
Sbjct: 554  INFVCAHDGFTLADLVTYNNKHNLANGEDNNDGESHNNSWNCGQ 597



 Score =  325 bits (832), Expect(2) = 0.0
 Identities = 147/181 (81%), Positives = 168/181 (92%)
 Frame = -3

Query: 716  IVVRGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKQESSSDFFRFCSLLTKFRRE 537
            +V +GVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKK+ESS DFFRFC L+TKFR+E
Sbjct: 622  MVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKEESSLDFFRFCCLMTKFRQE 681

Query: 536  CESLGLDNFPTADRLQWHGHFPGVPDWSETSRFVAFSLIDSVKRELYIAFNTSHLPVIIT 357
             ESLGL++FPTA+RLQWHGH PGVPDWSETSRFVAF+L+DSVKRELYIAFN SHLP  IT
Sbjct: 682  SESLGLNDFPTAERLQWHGHAPGVPDWSETSRFVAFTLMDSVKRELYIAFNASHLPATIT 741

Query: 356  LPKRPGYRWEPLVDTSKTSPFDFLSDDLPEKAIALEQYSHFLNADVYPMLSYSSIILLLT 177
            LP+RPGYRW+PLVDTSK +PFDFLS DLP + IA++QY+HFL+A++YPMLSYSSIIL L+
Sbjct: 742  LPERPGYRWDPLVDTSKPAPFDFLSSDLPGRDIAVKQYAHFLDANLYPMLSYSSIILFLS 801

Query: 176  P 174
            P
Sbjct: 802  P 802


>ref|XP_002324659.2| isoamylase family protein [Populus trichocarpa]
            gi|550318648|gb|EEF03224.2| isoamylase family protein
            [Populus trichocarpa]
          Length = 794

 Score =  882 bits (2280), Expect(2) = 0.0
 Identities = 415/524 (79%), Positives = 459/524 (87%), Gaps = 1/524 (0%)
 Frame = -2

Query: 2274 EVAETVVIEKPESRRFQVFNGRPLPFGATVRDGGVNFAIHSSGAVSATLCLISLSTLQEN 2095
            + A  V  E P+ R+FQVF G P PFGATVRDGGVNFAI S+ AVSATLCLISLS L EN
Sbjct: 62   DTAVVVEEEGPKLRKFQVFEGHPSPFGATVRDGGVNFAIFSANAVSATLCLISLSDLPEN 121

Query: 2094 RVTKKINLDPLINKTGDVWHVFLKGDFENMLYGYTFDGEFSPEEGYCYDSSKILLDPYAK 1915
            RVT++  LDPL NKTGDVWHV LKGDF++MLYGY FDG FSPEEG+ YD S+I+LDPYAK
Sbjct: 122  RVTEQFFLDPLTNKTGDVWHVHLKGDFKDMLYGYKFDGSFSPEEGHYYDPSQIVLDPYAK 181

Query: 1914 AVISREEFGALGSEGNCWSQMAGMVPYNDNEFNWEGDLPLRHPQRDLIVYEMHVRGFTRH 1735
            AVISR +FG LGS+ NCW QMA M+P  D++F+WEGD  L++PQRDLI+YEMHVRGFT+H
Sbjct: 182  AVISRGDFGVLGSDDNCWPQMACMIPTADDKFDWEGDSLLKYPQRDLILYEMHVRGFTQH 241

Query: 1734 DSSETDFPGTYLGVVEKLDHLKELGVNCIELMPFHEYNELEYFSYNSILGNYKLNFWGYS 1555
            +SS T+FPGTYLGVVEKL HLKELGVNCIELMP HE+NELEY+ YNS+ G+YK+NFWGYS
Sbjct: 242  ESSRTEFPGTYLGVVEKLGHLKELGVNCIELMPCHEFNELEYYDYNSVFGDYKVNFWGYS 301

Query: 1554 TVNFFSPMLRYSSAGIRNCGQDAINEVKILVREAHKRGIEVLMDVVFNHTAEGNEKGPML 1375
            TVN+FSPM RYSSAG  NCG DAINE K+LVREAHKRGIEV MDVVFNHTAEGNE+GP+L
Sbjct: 302  TVNYFSPMTRYSSAGTLNCGHDAINEFKLLVREAHKRGIEVFMDVVFNHTAEGNERGPIL 361

Query: 1374 SFRGVDNSVYYMLAPKGEYYNYSGCGNTFNCNHPVVRQFIVDCLRYWVEEMHVDGFRFDL 1195
            SF+GVDNSVYYMLAPKGE+YNYSGCGNTFNCNHPVVRQFI+DCLRYWV EMHVDGFRFDL
Sbjct: 362  SFKGVDNSVYYMLAPKGEFYNYSGCGNTFNCNHPVVRQFILDCLRYWVIEMHVDGFRFDL 421

Query: 1194 ASILTRSSSLWDAVNVYGNSTEGDMLTTGTPLGSPPLVEMISNDPILRHVKLIAEAWDTG 1015
            ASI+TRSSSLWDAVNV+G+  EGD++TTGTPLGSPPL+EM+SNDPILR VKLIAEAWD G
Sbjct: 422  ASIMTRSSSLWDAVNVFGSPIEGDLVTTGTPLGSPPLIEMMSNDPILRDVKLIAEAWDAG 481

Query: 1014 GLYQVGVFPHWGIWSEWNGKYRDIVRQFIKGTGGLSGAFAECLCGSPNLYQ-GARKPWNS 838
            GLYQVG+FPHW IWSEWNGKYRDIVRQFIKGT G SGAFAECLCGSP LYQ G RKPWNS
Sbjct: 482  GLYQVGMFPHWHIWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPGLYQEGGRKPWNS 541

Query: 837  INFVCAHDGFTLADLVTYNDKHNLAXXXXXXXXXXXXNSWNCGQ 706
            INFVCAHDGFTLADLVTYN+KHNLA            NSWNCGQ
Sbjct: 542  INFVCAHDGFTLADLVTYNEKHNLANGEDNNDGENHNNSWNCGQ 585



 Score =  321 bits (823), Expect(2) = 0.0
 Identities = 146/181 (80%), Positives = 164/181 (90%)
 Frame = -3

Query: 716  IVVRGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKQESSSDFFRFCSLLTKFRRE 537
            +V +GVPMI+MGDEYGHTKGGNNNTYCHDNYINYF WDKK+ESSSDFFRFC L+TKFR E
Sbjct: 610  MVSQGVPMIHMGDEYGHTKGGNNNTYCHDNYINYFWWDKKEESSSDFFRFCRLMTKFRHE 669

Query: 536  CESLGLDNFPTADRLQWHGHFPGVPDWSETSRFVAFSLIDSVKRELYIAFNTSHLPVIIT 357
            CESLGL++FPTA+RLQWHGH PG PDWSETSRFVAF+LIDSVKRE+YIAFN SHL V IT
Sbjct: 670  CESLGLNDFPTAERLQWHGHAPGTPDWSETSRFVAFTLIDSVKREIYIAFNASHLSVTIT 729

Query: 356  LPKRPGYRWEPLVDTSKTSPFDFLSDDLPEKAIALEQYSHFLNADVYPMLSYSSIILLLT 177
            LP+RP YRWEPLVDT K +PFDFLS DLPE+  A++QYSHFL A++YPMLSYSSIIL+L 
Sbjct: 730  LPERPAYRWEPLVDTGKPAPFDFLSGDLPERDTAMKQYSHFLEANLYPMLSYSSIILVLL 789

Query: 176  P 174
            P
Sbjct: 790  P 790


>ref|XP_011017747.1| PREDICTED: isoamylase 1, chloroplastic-like isoform X2 [Populus
            euphratica]
          Length = 766

 Score =  881 bits (2276), Expect(2) = 0.0
 Identities = 414/525 (78%), Positives = 460/525 (87%), Gaps = 1/525 (0%)
 Frame = -2

Query: 2274 EVAETVVIEKPESRRFQVFNGRPLPFGATVRDGGVNFAIHSSGAVSATLCLISLSTLQEN 2095
            + A  V  E P+ R+FQVF G P+PFGATVRDGGVNFAI S+ AVSATLCLISLS L EN
Sbjct: 62   DTAVVVEEEGPKLRKFQVFEGHPVPFGATVRDGGVNFAIFSANAVSATLCLISLSDLPEN 121

Query: 2094 RVTKKINLDPLINKTGDVWHVFLKGDFENMLYGYTFDGEFSPEEGYCYDSSKILLDPYAK 1915
             VT++  LDPL NKTGDVWHV LKGDF++MLYGY F G FSPEEG+ YD S+I+LDPYAK
Sbjct: 122  TVTEQFFLDPLTNKTGDVWHVHLKGDFKDMLYGYKFGGSFSPEEGHYYDPSQIVLDPYAK 181

Query: 1914 AVISREEFGALGSEGNCWSQMAGMVPYNDNEFNWEGDLPLRHPQRDLIVYEMHVRGFTRH 1735
            AVISR +FG  GS+ NCW QMA M+P  D++F+WEGD  L++PQRDLI+YEMHVRGFT+H
Sbjct: 182  AVISRGDFGVFGSDDNCWPQMACMIPTADDKFDWEGDSLLKYPQRDLIIYEMHVRGFTQH 241

Query: 1734 DSSETDFPGTYLGVVEKLDHLKELGVNCIELMPFHEYNELEYFSYNSILGNYKLNFWGYS 1555
            +SS T+FPGTYLGVVEKLDHLKELGVNCIELMP HE+NELEY+SYNS+LG+YK+NFWGYS
Sbjct: 242  ESSRTEFPGTYLGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYKVNFWGYS 301

Query: 1554 TVNFFSPMLRYSSAGIRNCGQDAINEVKILVREAHKRGIEVLMDVVFNHTAEGNEKGPML 1375
             VN+FSPM RYSSAG RNCG DAINE K+LVREAHKRGIEV MDVVFNHTAEGNE+GP+L
Sbjct: 302  PVNYFSPMTRYSSAGTRNCGHDAINEFKLLVREAHKRGIEVFMDVVFNHTAEGNERGPIL 361

Query: 1374 SFRGVDNSVYYMLAPKGEYYNYSGCGNTFNCNHPVVRQFIVDCLRYWVEEMHVDGFRFDL 1195
            SF+GVDNSVYYMLAPKGE+YNYSGCGNTFNCNHPVVRQFI+DCLRYWV EMHVDGFRFDL
Sbjct: 362  SFKGVDNSVYYMLAPKGEFYNYSGCGNTFNCNHPVVRQFILDCLRYWVIEMHVDGFRFDL 421

Query: 1194 ASILTRSSSLWDAVNVYGNSTEGDMLTTGTPLGSPPLVEMISNDPILRHVKLIAEAWDTG 1015
            ASI+TRSSSLWDAVNV+G+  EGD++TTGTPLGSPPL+EM+SNDPILR VKLIAEAWD G
Sbjct: 422  ASIMTRSSSLWDAVNVFGSPIEGDLVTTGTPLGSPPLIEMMSNDPILRDVKLIAEAWDAG 481

Query: 1014 GLYQVGVFPHWGIWSEWNGKYRDIVRQFIKGTGGLSGAFAECLCGSPNLYQ-GARKPWNS 838
            GLYQVG+FPHW IWSEWNGKYRDIVRQ IKGT G SGAFAECLCGSP+LYQ   RKPWNS
Sbjct: 482  GLYQVGMFPHWHIWSEWNGKYRDIVRQVIKGTDGFSGAFAECLCGSPDLYQERGRKPWNS 541

Query: 837  INFVCAHDGFTLADLVTYNDKHNLAXXXXXXXXXXXXNSWNCGQG 703
            INFVCAHDGFTLADLVTYN+KHNLA            NSWNCGQG
Sbjct: 542  INFVCAHDGFTLADLVTYNEKHNLANGEDNNDGENHNNSWNCGQG 586



 Score =  318 bits (816), Expect(2) = 0.0
 Identities = 144/178 (80%), Positives = 164/178 (92%)
 Frame = -3

Query: 707  RGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKQESSSDFFRFCSLLTKFRRECES 528
            +GVPMI+MGDEYGHTKGGNNNTYCHDNYINYF WDKK+ESSSDFFRFC L+TKFR ECES
Sbjct: 585  QGVPMIHMGDEYGHTKGGNNNTYCHDNYINYFWWDKKEESSSDFFRFCRLMTKFRHECES 644

Query: 527  LGLDNFPTADRLQWHGHFPGVPDWSETSRFVAFSLIDSVKRELYIAFNTSHLPVIITLPK 348
            LG ++FPTA+RLQWHGH PG PDWSETSRFVAF+LIDSVKRE+YIAFN S+LPVIITLP+
Sbjct: 645  LGSNDFPTAERLQWHGHAPGTPDWSETSRFVAFTLIDSVKREIYIAFNASYLPVIITLPE 704

Query: 347  RPGYRWEPLVDTSKTSPFDFLSDDLPEKAIALEQYSHFLNADVYPMLSYSSIILLLTP 174
            RP YRWEPLVDT+K +PF+FLS DLPE+  A++QYSHFL A++YPMLSYSSIIL+L P
Sbjct: 705  RPAYRWEPLVDTAKPAPFEFLSCDLPERDTAMKQYSHFLEANLYPMLSYSSIILVLLP 762


>ref|XP_011017745.1| PREDICTED: isoamylase 1, chloroplastic-like isoform X1 [Populus
            euphratica]
          Length = 794

 Score =  879 bits (2270), Expect(2) = 0.0
 Identities = 413/524 (78%), Positives = 459/524 (87%), Gaps = 1/524 (0%)
 Frame = -2

Query: 2274 EVAETVVIEKPESRRFQVFNGRPLPFGATVRDGGVNFAIHSSGAVSATLCLISLSTLQEN 2095
            + A  V  E P+ R+FQVF G P+PFGATVRDGGVNFAI S+ AVSATLCLISLS L EN
Sbjct: 62   DTAVVVEEEGPKLRKFQVFEGHPVPFGATVRDGGVNFAIFSANAVSATLCLISLSDLPEN 121

Query: 2094 RVTKKINLDPLINKTGDVWHVFLKGDFENMLYGYTFDGEFSPEEGYCYDSSKILLDPYAK 1915
             VT++  LDPL NKTGDVWHV LKGDF++MLYGY F G FSPEEG+ YD S+I+LDPYAK
Sbjct: 122  TVTEQFFLDPLTNKTGDVWHVHLKGDFKDMLYGYKFGGSFSPEEGHYYDPSQIVLDPYAK 181

Query: 1914 AVISREEFGALGSEGNCWSQMAGMVPYNDNEFNWEGDLPLRHPQRDLIVYEMHVRGFTRH 1735
            AVISR +FG  GS+ NCW QMA M+P  D++F+WEGD  L++PQRDLI+YEMHVRGFT+H
Sbjct: 182  AVISRGDFGVFGSDDNCWPQMACMIPTADDKFDWEGDSLLKYPQRDLIIYEMHVRGFTQH 241

Query: 1734 DSSETDFPGTYLGVVEKLDHLKELGVNCIELMPFHEYNELEYFSYNSILGNYKLNFWGYS 1555
            +SS T+FPGTYLGVVEKLDHLKELGVNCIELMP HE+NELEY+SYNS+LG+YK+NFWGYS
Sbjct: 242  ESSRTEFPGTYLGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYKVNFWGYS 301

Query: 1554 TVNFFSPMLRYSSAGIRNCGQDAINEVKILVREAHKRGIEVLMDVVFNHTAEGNEKGPML 1375
             VN+FSPM RYSSAG RNCG DAINE K+LVREAHKRGIEV MDVVFNHTAEGNE+GP+L
Sbjct: 302  PVNYFSPMTRYSSAGTRNCGHDAINEFKLLVREAHKRGIEVFMDVVFNHTAEGNERGPIL 361

Query: 1374 SFRGVDNSVYYMLAPKGEYYNYSGCGNTFNCNHPVVRQFIVDCLRYWVEEMHVDGFRFDL 1195
            SF+GVDNSVYYMLAPKGE+YNYSGCGNTFNCNHPVVRQFI+DCLRYWV EMHVDGFRFDL
Sbjct: 362  SFKGVDNSVYYMLAPKGEFYNYSGCGNTFNCNHPVVRQFILDCLRYWVIEMHVDGFRFDL 421

Query: 1194 ASILTRSSSLWDAVNVYGNSTEGDMLTTGTPLGSPPLVEMISNDPILRHVKLIAEAWDTG 1015
            ASI+TRSSSLWDAVNV+G+  EGD++TTGTPLGSPPL+EM+SNDPILR VKLIAEAWD G
Sbjct: 422  ASIMTRSSSLWDAVNVFGSPIEGDLVTTGTPLGSPPLIEMMSNDPILRDVKLIAEAWDAG 481

Query: 1014 GLYQVGVFPHWGIWSEWNGKYRDIVRQFIKGTGGLSGAFAECLCGSPNLYQ-GARKPWNS 838
            GLYQVG+FPHW IWSEWNGKYRDIVRQ IKGT G SGAFAECLCGSP+LYQ   RKPWNS
Sbjct: 482  GLYQVGMFPHWHIWSEWNGKYRDIVRQVIKGTDGFSGAFAECLCGSPDLYQERGRKPWNS 541

Query: 837  INFVCAHDGFTLADLVTYNDKHNLAXXXXXXXXXXXXNSWNCGQ 706
            INFVCAHDGFTLADLVTYN+KHNLA            NSWNCGQ
Sbjct: 542  INFVCAHDGFTLADLVTYNEKHNLANGEDNNDGENHNNSWNCGQ 585



 Score =  320 bits (819), Expect(2) = 0.0
 Identities = 145/181 (80%), Positives = 166/181 (91%)
 Frame = -3

Query: 716  IVVRGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKQESSSDFFRFCSLLTKFRRE 537
            +V +GVPMI+MGDEYGHTKGGNNNTYCHDNYINYF WDKK+ESSSDFFRFC L+TKFR E
Sbjct: 610  MVSQGVPMIHMGDEYGHTKGGNNNTYCHDNYINYFWWDKKEESSSDFFRFCRLMTKFRHE 669

Query: 536  CESLGLDNFPTADRLQWHGHFPGVPDWSETSRFVAFSLIDSVKRELYIAFNTSHLPVIIT 357
            CESLG ++FPTA+RLQWHGH PG PDWSETSRFVAF+LIDSVKRE+YIAFN S+LPVIIT
Sbjct: 670  CESLGSNDFPTAERLQWHGHAPGTPDWSETSRFVAFTLIDSVKREIYIAFNASYLPVIIT 729

Query: 356  LPKRPGYRWEPLVDTSKTSPFDFLSDDLPEKAIALEQYSHFLNADVYPMLSYSSIILLLT 177
            LP+RP YRWEPLVDT+K +PF+FLS DLPE+  A++QYSHFL A++YPMLSYSSIIL+L 
Sbjct: 730  LPERPAYRWEPLVDTAKPAPFEFLSCDLPERDTAMKQYSHFLEANLYPMLSYSSIILVLL 789

Query: 176  P 174
            P
Sbjct: 790  P 790


>ref|NP_001274937.1| isoamylase isoform 1 [Solanum tuberosum] gi|27728145|gb|AAN15317.1|
            isoamylase isoform 1 [Solanum tuberosum]
          Length = 793

 Score =  877 bits (2266), Expect(2) = 0.0
 Identities = 410/524 (78%), Positives = 459/524 (87%), Gaps = 3/524 (0%)
 Frame = -2

Query: 2268 AETVVIEKP--ESRRFQVFNGRPLPFGATVRDGGVNFAIHSSGAVSATLCLISLSTLQEN 2095
            A  VV+EKP  E  RF+V +G+PLPFGAT  DGGVNFA+ S  A +ATLCLI+LS L E 
Sbjct: 61   ATAVVVEKPTTERCRFEVLSGKPLPFGATATDGGVNFAVFSRNATAATLCLITLSDLPEK 120

Query: 2094 RVTKKINLDPLINKTGDVWHVFLKGDFENMLYGYTFDGEFSPEEGYCYDSSKILLDPYAK 1915
            RVT++I LDPL NKTGDVWHVFLKGDFENMLYGY FDG+F PEEG+ +DSS+I+LDPYAK
Sbjct: 121  RVTEQIFLDPLANKTGDVWHVFLKGDFENMLYGYKFDGKFCPEEGHYFDSSQIVLDPYAK 180

Query: 1914 AVISREEFGALGSEGNCWSQMAGMVPYNDNEFNWEGDLPLRHPQRDLIVYEMHVRGFTRH 1735
            A++SR E+G LG E +CW  MAGMVP   ++F+WEGDLPL+ PQRDL++YEMHVRGFT H
Sbjct: 181  AIVSRGEYGVLGPEDDCWPPMAGMVPSASDQFDWEGDLPLKFPQRDLVIYEMHVRGFTNH 240

Query: 1734 DSSETDFPGTYLGVVEKLDHLKELGVNCIELMPFHEYNELEYFSYNSILGNYKLNFWGYS 1555
            +SSET +PGTYLGVVEKLDHLKELGVNCIELMP HE+NELEY+SYNS+LG+YK NFWGYS
Sbjct: 241  ESSETKYPGTYLGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYKFNFWGYS 300

Query: 1554 TVNFFSPMLRYSSAGIRNCGQDAINEVKILVREAHKRGIEVLMDVVFNHTAEGNEKGPML 1375
            TVNFFSPM RYSSAG+ NCG  AINE K LV+EAHKRGIEV+MDVVFNHTAEGNE GP+L
Sbjct: 301  TVNFFSPMGRYSSAGLSNCGLGAINEFKYLVKEAHKRGIEVIMDVVFNHTAEGNENGPIL 360

Query: 1374 SFRGVDNSVYYMLAPKGEYYNYSGCGNTFNCNHPVVRQFIVDCLRYWVEEMHVDGFRFDL 1195
            SFRG+DNSV+Y LAPKGE+YNYSGCGNTFNCN+P+VRQFIVDCLRYWV EMHVDGFRFDL
Sbjct: 361  SFRGIDNSVFYTLAPKGEFYNYSGCGNTFNCNNPIVRQFIVDCLRYWVTEMHVDGFRFDL 420

Query: 1194 ASILTRSSSLWDAVNVYGNSTEGDMLTTGTPLGSPPLVEMISNDPILRHVKLIAEAWDTG 1015
            ASILTRSSS W+AVNVYGNS +GD++TTGTPL SPPL++MISNDPILR VKLIAEAWD G
Sbjct: 421  ASILTRSSSSWNAVNVYGNSIDGDVITTGTPLTSPPLIDMISNDPILRGVKLIAEAWDCG 480

Query: 1014 GLYQVGVFPHWGIWSEWNGKYRDIVRQFIKGTGGLSGAFAECLCGSPNLYQ-GARKPWNS 838
            GLYQVG+FPHWGIWSEWNGKYRD+VRQFIKGT G SGAFAECLCGSPNLYQ G RKPWNS
Sbjct: 481  GLYQVGMFPHWGIWSEWNGKYRDMVRQFIKGTDGFSGAFAECLCGSPNLYQKGGRKPWNS 540

Query: 837  INFVCAHDGFTLADLVTYNDKHNLAXXXXXXXXXXXXNSWNCGQ 706
            INFVCAHDGFTLADLVTYN+KHNLA            NSWNCG+
Sbjct: 541  INFVCAHDGFTLADLVTYNNKHNLANGEDNKDGENHNNSWNCGE 584



 Score =  319 bits (817), Expect(2) = 0.0
 Identities = 145/184 (78%), Positives = 160/184 (86%)
 Frame = -3

Query: 716  IVVRGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKQESSSDFFRFCSLLTKFRRE 537
            +V +GVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKK ESSSDF RFC L+TKFR E
Sbjct: 609  MVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKDESSSDFLRFCGLMTKFRHE 668

Query: 536  CESLGLDNFPTADRLQWHGHFPGVPDWSETSRFVAFSLIDSVKRELYIAFNTSHLPVIIT 357
            CESLGLD FPTA+RLQWHGH P  PDWSETSRFVAF+L+D VK ELYIAFN SHLPV IT
Sbjct: 669  CESLGLDGFPTAERLQWHGHTPRTPDWSETSRFVAFTLVDKVKGELYIAFNASHLPVTIT 728

Query: 356  LPKRPGYRWEPLVDTSKTSPFDFLSDDLPEKAIALEQYSHFLNADVYPMLSYSSIILLLT 177
            LP RPGYRW+P VDT K +PFDFL+DD+PE+  A +QYSHFL+A+ YPMLSYSSIILLL+
Sbjct: 729  LPDRPGYRWQPFVDTGKPAPFDFLTDDVPERETAAKQYSHFLDANQYPMLSYSSIILLLS 788

Query: 176  PDGD 165
               D
Sbjct: 789  SADD 792


>ref|XP_009588914.1| PREDICTED: isoamylase 1, chloroplastic [Nicotiana tomentosiformis]
          Length = 793

 Score =  875 bits (2262), Expect(2) = 0.0
 Identities = 412/524 (78%), Positives = 455/524 (86%), Gaps = 3/524 (0%)
 Frame = -2

Query: 2268 AETVVIEKP--ESRRFQVFNGRPLPFGATVRDGGVNFAIHSSGAVSATLCLISLSTLQEN 2095
            A  VV+EKP  E  RF+V  G+PLPFGAT  DGGVNFA+ SS A SATLCLI+LS L + 
Sbjct: 61   ATAVVVEKPMIERHRFEVLPGKPLPFGATATDGGVNFAVFSSHATSATLCLITLSDLPQK 120

Query: 2094 RVTKKINLDPLINKTGDVWHVFLKGDFENMLYGYTFDGEFSPEEGYCYDSSKILLDPYAK 1915
            RVT +I LDPL NKTGDVWHVFLKGDFENMLYGY FDG+FSPEEG+ +DSS+I+LDPYAK
Sbjct: 121  RVTVQIFLDPLANKTGDVWHVFLKGDFENMLYGYKFDGKFSPEEGHFFDSSQIVLDPYAK 180

Query: 1914 AVISREEFGALGSEGNCWSQMAGMVPYNDNEFNWEGDLPLRHPQRDLIVYEMHVRGFTRH 1735
            A +SR E+G LG E +CW  MAGMVP   ++F+WEGDLPL+ PQRD+++YEMHVRG+T H
Sbjct: 181  ATVSRGEYGVLGPEDDCWPPMAGMVPSAADQFDWEGDLPLKFPQRDVVIYEMHVRGYTNH 240

Query: 1734 DSSETDFPGTYLGVVEKLDHLKELGVNCIELMPFHEYNELEYFSYNSILGNYKLNFWGYS 1555
            +SS T FPGTY GVVEKLDHLKELGVNCIELMP HE+NELEY+SYNS+LG+YK NFWGYS
Sbjct: 241  ESSGTKFPGTYRGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYKFNFWGYS 300

Query: 1554 TVNFFSPMLRYSSAGIRNCGQDAINEVKILVREAHKRGIEVLMDVVFNHTAEGNEKGPML 1375
            TVNFFSPM RYSSAG+ NCG  AINE K LV+EAHKRGIEV+MDVVFNHTAEGNE GP++
Sbjct: 301  TVNFFSPMGRYSSAGLSNCGLGAINEFKYLVKEAHKRGIEVIMDVVFNHTAEGNENGPIV 360

Query: 1374 SFRGVDNSVYYMLAPKGEYYNYSGCGNTFNCNHPVVRQFIVDCLRYWVEEMHVDGFRFDL 1195
            SFRGVDNSV+YMLAPKGE+YNYSGCGNTFNCNHP+VRQFIVDCLRYWV EMHVDGFRFDL
Sbjct: 361  SFRGVDNSVFYMLAPKGEFYNYSGCGNTFNCNHPIVRQFIVDCLRYWVTEMHVDGFRFDL 420

Query: 1194 ASILTRSSSLWDAVNVYGNSTEGDMLTTGTPLGSPPLVEMISNDPILRHVKLIAEAWDTG 1015
            ASILTRSSSLWDAVNVYGNST+ D++TTGTPL +PPL++MISNDPILR VKLIAEAWD G
Sbjct: 421  ASILTRSSSLWDAVNVYGNSTDVDVITTGTPLTNPPLIDMISNDPILRGVKLIAEAWDCG 480

Query: 1014 GLYQVGVFPHWGIWSEWNGKYRDIVRQFIKGTGGLSGAFAECLCGSPNLYQ-GARKPWNS 838
            GLYQVG FPHWGIWSEWNGKYRDIVRQFIKGT G SGA AECLCGSPNLYQ G R PWNS
Sbjct: 481  GLYQVGTFPHWGIWSEWNGKYRDIVRQFIKGTDGFSGALAECLCGSPNLYQEGGRNPWNS 540

Query: 837  INFVCAHDGFTLADLVTYNDKHNLAXXXXXXXXXXXXNSWNCGQ 706
            INFVCAHDGFTLADLVTYNDKHNLA            NSWNCG+
Sbjct: 541  INFVCAHDGFTLADLVTYNDKHNLANGEDNKDGENHNNSWNCGE 584



 Score =  318 bits (815), Expect(2) = 0.0
 Identities = 145/184 (78%), Positives = 161/184 (87%)
 Frame = -3

Query: 716  IVVRGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKQESSSDFFRFCSLLTKFRRE 537
            +V +GVPMI MGDEYGHTKGGNNNTYCHDNYINYFRWDKK ESSSDFFRFC L+TKFR E
Sbjct: 609  MVSQGVPMICMGDEYGHTKGGNNNTYCHDNYINYFRWDKKDESSSDFFRFCCLVTKFRHE 668

Query: 536  CESLGLDNFPTADRLQWHGHFPGVPDWSETSRFVAFSLIDSVKRELYIAFNTSHLPVIIT 357
            CESLGL  FPTA+RLQWHGH PG PDWSETSRFVAF+L+D VK ELY+AFN SHLPV IT
Sbjct: 669  CESLGLGGFPTAERLQWHGHAPGTPDWSETSRFVAFTLVDKVKGELYVAFNASHLPVTIT 728

Query: 356  LPKRPGYRWEPLVDTSKTSPFDFLSDDLPEKAIALEQYSHFLNADVYPMLSYSSIILLLT 177
            LP RPGYRW+PLVDT K +P+DFL+DDLP +  A +QYSHFL+A+ YPMLSYSSIILLL+
Sbjct: 729  LPDRPGYRWQPLVDTGKPAPYDFLTDDLPGRETAAKQYSHFLDANQYPMLSYSSIILLLS 788

Query: 176  PDGD 165
             D D
Sbjct: 789  IDDD 792


>ref|XP_009771255.1| PREDICTED: isoamylase 1, chloroplastic [Nicotiana sylvestris]
          Length = 792

 Score =  875 bits (2260), Expect(2) = 0.0
 Identities = 416/533 (78%), Positives = 461/533 (86%), Gaps = 4/533 (0%)
 Frame = -2

Query: 2292 GVNSTGEVAET-VVIEKP--ESRRFQVFNGRPLPFGATVRDGGVNFAIHSSGAVSATLCL 2122
            G  +  E A T VV+EKP  E RRF+V  G+PLPFGAT  DGGVNFA+ SS A SATLCL
Sbjct: 52   GRGAKAETAATAVVVEKPTMERRRFEVLPGKPLPFGATATDGGVNFAVFSSNATSATLCL 111

Query: 2121 ISLSTLQENRVTKKINLDPLINKTGDVWHVFLKGDFENMLYGYTFDGEFSPEEGYCYDSS 1942
            I+LS L + RVT +I LDPL NKTGDVWHVFLKGDFENMLYGY FDG+FSPE G+ +DSS
Sbjct: 112  ITLSDLPQKRVTVQIFLDPLANKTGDVWHVFLKGDFENMLYGYKFDGKFSPE-GHFFDSS 170

Query: 1941 KILLDPYAKAVISREEFGALGSEGNCWSQMAGMVPYNDNEFNWEGDLPLRHPQRDLIVYE 1762
            +I+LDPYAKA +SR E+G LG E +CW  MAGMVP   ++F+WEGDLPL+ PQRDL++YE
Sbjct: 171  QIVLDPYAKATVSRGEYGVLGPEDDCWPLMAGMVPSAADQFDWEGDLPLKFPQRDLVIYE 230

Query: 1761 MHVRGFTRHDSSETDFPGTYLGVVEKLDHLKELGVNCIELMPFHEYNELEYFSYNSILGN 1582
            MHVRG+T H+SS T FPGTY GVVEKLDHLKELGVNCIELMP HE+NELEY+SYNS+LG+
Sbjct: 231  MHVRGYTNHESSGTKFPGTYRGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGD 290

Query: 1581 YKLNFWGYSTVNFFSPMLRYSSAGIRNCGQDAINEVKILVREAHKRGIEVLMDVVFNHTA 1402
            YK NFWGYSTVNFFSPM RYSSAG+ NCG  AINE K LV+EAHKRGIEV+MDVVFNHTA
Sbjct: 291  YKFNFWGYSTVNFFSPMGRYSSAGLSNCGLVAINEFKYLVKEAHKRGIEVIMDVVFNHTA 350

Query: 1401 EGNEKGPMLSFRGVDNSVYYMLAPKGEYYNYSGCGNTFNCNHPVVRQFIVDCLRYWVEEM 1222
            EGNE GP++SFRG+DNSV+YMLAPKGE+YNYSGCGNTFNCNHP+VRQFIVDCLRYWV EM
Sbjct: 351  EGNENGPIVSFRGIDNSVFYMLAPKGEFYNYSGCGNTFNCNHPIVRQFIVDCLRYWVTEM 410

Query: 1221 HVDGFRFDLASILTRSSSLWDAVNVYGNSTEGDMLTTGTPLGSPPLVEMISNDPILRHVK 1042
            H+DGFRFDLASILTRSSSLWDAVNVYGNST+ D++TTGTPL SPPL++MISNDPILR VK
Sbjct: 411  HIDGFRFDLASILTRSSSLWDAVNVYGNSTDVDVITTGTPLTSPPLIDMISNDPILRGVK 470

Query: 1041 LIAEAWDTGGLYQVGVFPHWGIWSEWNGKYRDIVRQFIKGTGGLSGAFAECLCGSPNLYQ 862
            LIAEAWD GGLYQVG+FPHWGIWSEWNGKYRDIVRQFIKGT G SGA AECLCGSPNLYQ
Sbjct: 471  LIAEAWDCGGLYQVGMFPHWGIWSEWNGKYRDIVRQFIKGTDGFSGALAECLCGSPNLYQ 530

Query: 861  -GARKPWNSINFVCAHDGFTLADLVTYNDKHNLAXXXXXXXXXXXXNSWNCGQ 706
             G RKPWNSINFVCAHDGFTLADLVTYNDKHNLA            NSWNCG+
Sbjct: 531  EGGRKPWNSINFVCAHDGFTLADLVTYNDKHNLANGEDNKDGENHNNSWNCGE 583



 Score =  327 bits (837), Expect(2) = 0.0
 Identities = 147/184 (79%), Positives = 164/184 (89%)
 Frame = -3

Query: 716  IVVRGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKQESSSDFFRFCSLLTKFRRE 537
            +V +GVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKK ESSSDFFRFC L+TKFR +
Sbjct: 608  MVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKDESSSDFFRFCCLVTKFRHK 667

Query: 536  CESLGLDNFPTADRLQWHGHFPGVPDWSETSRFVAFSLIDSVKRELYIAFNTSHLPVIIT 357
            CESLGLD FPTA+RLQWHGH PG PDWSETSRFVAF+L+D VK ELY+AFN SHLPV IT
Sbjct: 668  CESLGLDGFPTAERLQWHGHAPGTPDWSETSRFVAFTLVDKVKGELYVAFNASHLPVTIT 727

Query: 356  LPKRPGYRWEPLVDTSKTSPFDFLSDDLPEKAIALEQYSHFLNADVYPMLSYSSIILLLT 177
            LP RPGYRW+PLVDT K +P+DFL+DDLP +  A +QYSHFL+A+ YPMLSYSSIILLL+
Sbjct: 728  LPDRPGYRWQPLVDTGKPAPYDFLTDDLPGRETAAKQYSHFLDANQYPMLSYSSIILLLS 787

Query: 176  PDGD 165
            PD D
Sbjct: 788  PDDD 791


>ref|XP_010688426.1| PREDICTED: isoamylase 1, chloroplastic [Beta vulgaris subsp.
            vulgaris] gi|870851207|gb|KMT03273.1| hypothetical
            protein BVRB_8g198150 [Beta vulgaris subsp. vulgaris]
          Length = 795

 Score =  874 bits (2259), Expect(2) = 0.0
 Identities = 404/527 (76%), Positives = 463/527 (87%), Gaps = 2/527 (0%)
 Frame = -2

Query: 2280 TGEVAETVVIEK-PESRRFQVFNGRPLPFGATVRDGGVNFAIHSSGAVSATLCLISLSTL 2104
            T    +TVVI K PE+   ++  G P PFGA VRDGGVNFAI S  +VSATLCLI+LS L
Sbjct: 58   TSTEIDTVVISKSPETSLLEISEGYPAPFGAIVRDGGVNFAIFSGHSVSATLCLITLSDL 117

Query: 2103 QENRVTKKINLDPLINKTGDVWHVFLKGDFENMLYGYTFDGEFSPEEGYCYDSSKILLDP 1924
            Q+N +T++I+LDPLINKTGDVWHVFLKG+F NMLYGY FDG F PEEG+ YDSS+I+LDP
Sbjct: 118  QKNIITEQISLDPLINKTGDVWHVFLKGNFNNMLYGYKFDGPFLPEEGHYYDSSRIILDP 177

Query: 1923 YAKAVISREEFGALGSEGNCWSQMAGMVPYNDNEFNWEGDLPLRHPQRDLIVYEMHVRGF 1744
            YAKAVISR ++G LG E NCW  MAGM+P  ++EF+W+GDLPL+HPQR+LI+YEMHVRGF
Sbjct: 178  YAKAVISRGDYGELGPEDNCWPLMAGMIPSANDEFDWQGDLPLKHPQRELIIYEMHVRGF 237

Query: 1743 TRHDSSETDFPGTYLGVVEKLDHLKELGVNCIELMPFHEYNELEYFSYNSILGNYKLNFW 1564
            T+H+SS T+ PGTY G++EKLDHLKELG+NCIELMP HE+NELEY+SYNS +G+YK+NFW
Sbjct: 238  TKHESSRTNCPGTYAGMIEKLDHLKELGINCIELMPCHEFNELEYYSYNSAMGDYKMNFW 297

Query: 1563 GYSTVNFFSPMLRYSSAGIRNCGQDAINEVKILVREAHKRGIEVLMDVVFNHTAEGNEKG 1384
            GYST+N+FSPM RYSS+GIRNCG++AINE K+LVREAHKRGIEV+MDVVFNHTAEGN+KG
Sbjct: 298  GYSTINYFSPMTRYSSSGIRNCGREAINEFKLLVREAHKRGIEVIMDVVFNHTAEGNQKG 357

Query: 1383 PMLSFRGVDNSVYYMLAPKGEYYNYSGCGNTFNCNHPVVRQFIVDCLRYWVEEMHVDGFR 1204
            P LSFRG+DNSV+YMLAPKGE+YNYSGCGNTFNCNHPVVRQFIVDCLRYWV EMHVDGFR
Sbjct: 358  PTLSFRGIDNSVFYMLAPKGEFYNYSGCGNTFNCNHPVVRQFIVDCLRYWVTEMHVDGFR 417

Query: 1203 FDLASILTRSSSLWDAVNVYGNSTEGDMLTTGTPLGSPPLVEMISNDPILRHVKLIAEAW 1024
            FDLASI+TR SSLWDAVNVYGN+TEGD+LTTGTPL SPPL++MISNDP+L  VKLIAEAW
Sbjct: 418  FDLASIMTRGSSLWDAVNVYGNATEGDLLTTGTPLSSPPLLDMISNDPVLCRVKLIAEAW 477

Query: 1023 DTGGLYQVGVFPHWGIWSEWNGKYRDIVRQFIKGTGGLSGAFAECLCGSPNLY-QGARKP 847
            D GGLYQVG FPHWGIWSEWNGKYRD+VR FIKGT G +GAFAECLCGSPNLY +G R+P
Sbjct: 478  DCGGLYQVGGFPHWGIWSEWNGKYRDMVRLFIKGTDGFAGAFAECLCGSPNLYKEGGRRP 537

Query: 846  WNSINFVCAHDGFTLADLVTYNDKHNLAXXXXXXXXXXXXNSWNCGQ 706
            W+SINFVCAHDGFTLADLVTYN+KHNLA            NSWNCGQ
Sbjct: 538  WHSINFVCAHDGFTLADLVTYNEKHNLANGENNMDGENHNNSWNCGQ 584



 Score =  318 bits (814), Expect(2) = 0.0
 Identities = 141/184 (76%), Positives = 165/184 (89%)
 Frame = -3

Query: 716  IVVRGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKQESSSDFFRFCSLLTKFRRE 537
            +V +GVPMIYMGDEYGHTKGGNNN+YCHDNYINYFRWDKK+E++SDFFRFC L+TKFR E
Sbjct: 609  MVSQGVPMIYMGDEYGHTKGGNNNSYCHDNYINYFRWDKKEEATSDFFRFCRLMTKFRSE 668

Query: 536  CESLGLDNFPTADRLQWHGHFPGVPDWSETSRFVAFSLIDSVKRELYIAFNTSHLPVIIT 357
            CESLGL+ FPT++RLQWHGH PG+PDWSETSRFVAF+L D V  E+Y+AFNTSHLPV +T
Sbjct: 669  CESLGLNEFPTSERLQWHGHIPGLPDWSETSRFVAFTLRDLVMGEIYVAFNTSHLPVTVT 728

Query: 356  LPKRPGYRWEPLVDTSKTSPFDFLSDDLPEKAIALEQYSHFLNADVYPMLSYSSIILLLT 177
            LP RPGYRWEPLVDTSK +PFDFLSDD+ E+  A+ Q+ HFL+A++YPMLSYSS+ILLL 
Sbjct: 729  LPDRPGYRWEPLVDTSKPTPFDFLSDDVTERETAIVQFQHFLDANMYPMLSYSSVILLLK 788

Query: 176  PDGD 165
            PD D
Sbjct: 789  PDID 792


>ref|XP_004242868.1| PREDICTED: isoamylase 1, chloroplastic isoform X2 [Solanum
            lycopersicum]
          Length = 787

 Score =  869 bits (2246), Expect(2) = 0.0
 Identities = 407/524 (77%), Positives = 457/524 (87%), Gaps = 3/524 (0%)
 Frame = -2

Query: 2268 AETVVIEKP--ESRRFQVFNGRPLPFGATVRDGGVNFAIHSSGAVSATLCLISLSTLQEN 2095
            A  VV+EK   E  RF+V +G+PLPFGAT  DGGVNFA+ S  A +ATLCLI+LS L E 
Sbjct: 55   ASAVVVEKSTTERCRFEVLSGKPLPFGATATDGGVNFAVFSRNATAATLCLITLSDLPEK 114

Query: 2094 RVTKKINLDPLINKTGDVWHVFLKGDFENMLYGYTFDGEFSPEEGYCYDSSKILLDPYAK 1915
            RVT++I LDPL NKTGDVWHVFLKGDFENMLYGY FDG+F PEEG+ +DSS+I+LDPYAK
Sbjct: 115  RVTEQIFLDPLANKTGDVWHVFLKGDFENMLYGYKFDGKFCPEEGHYFDSSQIVLDPYAK 174

Query: 1914 AVISREEFGALGSEGNCWSQMAGMVPYNDNEFNWEGDLPLRHPQRDLIVYEMHVRGFTRH 1735
            A++SR E+G LG E +CW  MAGMVP   ++F+WEGDLPL+  QR+L++YEMHVRGFT H
Sbjct: 175  AIVSRGEYGVLGPEDDCWPPMAGMVPSASDQFDWEGDLPLKFSQRNLVIYEMHVRGFTNH 234

Query: 1734 DSSETDFPGTYLGVVEKLDHLKELGVNCIELMPFHEYNELEYFSYNSILGNYKLNFWGYS 1555
            +SSET +PGTYLGVVEKLDHLKELGVNCIELMP HE+NELEY+SYNS+LG+YK NFWGYS
Sbjct: 235  ESSETKYPGTYLGVVEKLDHLKELGVNCIELMPCHEFNELEYYSYNSVLGDYKFNFWGYS 294

Query: 1554 TVNFFSPMLRYSSAGIRNCGQDAINEVKILVREAHKRGIEVLMDVVFNHTAEGNEKGPML 1375
            TVNFFSPM RYSSAG+ NCG  AINE K LV+EAHKRGIEV+MDVVFNHTAEGNE GP+L
Sbjct: 295  TVNFFSPMGRYSSAGLSNCGLGAINEFKYLVKEAHKRGIEVIMDVVFNHTAEGNENGPIL 354

Query: 1374 SFRGVDNSVYYMLAPKGEYYNYSGCGNTFNCNHPVVRQFIVDCLRYWVEEMHVDGFRFDL 1195
            SFRG+DNSV+Y LAPKGE+YNYSGCGNTFNCN+P+VRQFIVDCLRYWV EMHVDGFRFDL
Sbjct: 355  SFRGIDNSVFYTLAPKGEFYNYSGCGNTFNCNNPIVRQFIVDCLRYWVTEMHVDGFRFDL 414

Query: 1194 ASILTRSSSLWDAVNVYGNSTEGDMLTTGTPLGSPPLVEMISNDPILRHVKLIAEAWDTG 1015
            ASILTRSSS W+AVNVYGNS +GD++TTGTPL SPPL++MISNDPILR VKLIAEAWD G
Sbjct: 415  ASILTRSSSSWNAVNVYGNSIDGDVITTGTPLTSPPLIDMISNDPILRGVKLIAEAWDCG 474

Query: 1014 GLYQVGVFPHWGIWSEWNGKYRDIVRQFIKGTGGLSGAFAECLCGSPNLYQ-GARKPWNS 838
            GLYQVG+FPHWGIWSEWNGKYRD+VRQFIKGT G SGAFAECLCGSPNLYQ G RKPWNS
Sbjct: 475  GLYQVGMFPHWGIWSEWNGKYRDMVRQFIKGTDGFSGAFAECLCGSPNLYQKGGRKPWNS 534

Query: 837  INFVCAHDGFTLADLVTYNDKHNLAXXXXXXXXXXXXNSWNCGQ 706
            INFVCAHDGFTLADLVTYN+KHNLA            NSWNCG+
Sbjct: 535  INFVCAHDGFTLADLVTYNNKHNLANGEDNKDGENHNNSWNCGE 578



 Score =  318 bits (814), Expect(2) = 0.0
 Identities = 145/184 (78%), Positives = 161/184 (87%)
 Frame = -3

Query: 716  IVVRGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKQESSSDFFRFCSLLTKFRRE 537
            +V +GVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKK ESSSDF RFCSL+T FR E
Sbjct: 603  MVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKKDESSSDFLRFCSLMTNFRHE 662

Query: 536  CESLGLDNFPTADRLQWHGHFPGVPDWSETSRFVAFSLIDSVKRELYIAFNTSHLPVIIT 357
            CESLGLD FPTA+RLQWHGH P  PDWSETSRFVAF+L+D VK ELYIAFN SHLPV IT
Sbjct: 663  CESLGLDGFPTAERLQWHGHTPRTPDWSETSRFVAFTLVDKVKGELYIAFNASHLPVTIT 722

Query: 356  LPKRPGYRWEPLVDTSKTSPFDFLSDDLPEKAIALEQYSHFLNADVYPMLSYSSIILLLT 177
            LP+RPGY W+PLVDT K +PFDFL+DD+PE+  A +QYSHFL+A+ YPMLSYSSIILLL+
Sbjct: 723  LPERPGYLWQPLVDTGKPAPFDFLTDDVPERDTAAKQYSHFLDANQYPMLSYSSIILLLS 782

Query: 176  PDGD 165
               D
Sbjct: 783  SADD 786