BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Aconitum21_contig00024632
         (445 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcocla...   221   4e-56
gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chi...   211   5e-53
gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum...   209   1e-52
gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [P...   196   2e-48
gb|AAF05621.1|AF191772_1 (S)-N-methylcoclaurine 3'-hydroxylase [...   192   2e-47

>sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase
           isozyme 2; AltName: Full=Cytochrome P450 80B2
           gi|9971208|dbj|BAB12433.1|
           (S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
          Length = 488

 Score =  221 bits (564), Expect = 4e-56
 Identities = 108/134 (80%), Positives = 123/134 (91%), Gaps = 1/134 (0%)
 Frame = +2

Query: 47  GNSTNLADYFPVLGSLDLFGQRRAVAEVLKQIYDVWGAMLKERRGAKG-DCRNDFVNVLI 223
           GNSTN ADYFP++G LDLFGQRRAVAEVL+QIYDVWG MLKERRG KG + +NDFV+VL+
Sbjct: 211 GNSTNPADYFPIMGKLDLFGQRRAVAEVLQQIYDVWGVMLKERRGTKGSESKNDFVDVLL 270

Query: 224 DSGLDDQKINALLMELFGAGTETSASTIEWAIAELTKNPRITSKLQSELLNVVGENTVKE 403
           ++GLDDQKINALLMELFGAGTETSASTIEWAI ELTK P + SK++ EL+NVVG+NTVKE
Sbjct: 271 NAGLDDQKINALLMELFGAGTETSASTIEWAITELTKKPLVVSKIRLELVNVVGDNTVKE 330

Query: 404 SDIPHLPYLQAFVK 445
           SD+PHLPYLQAFVK
Sbjct: 331 SDLPHLPYLQAFVK 344


>gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
          Length = 488

 Score =  211 bits (537), Expect = 5e-53
 Identities = 103/134 (76%), Positives = 119/134 (88%), Gaps = 1/134 (0%)
 Frame = +2

Query: 47  GNSTNLADYFPVLGSLDLFGQRRAVAEVLKQIYDVWGAMLKERRGAKG-DCRNDFVNVLI 223
           GNSTN ADYFP++G  DLFGQRRAVA+VL+QIYDVWG  LKERRG KG +  NDFV+VL+
Sbjct: 211 GNSTNPADYFPIMGKYDLFGQRRAVADVLQQIYDVWGINLKERRGMKGSESNNDFVDVLL 270

Query: 224 DSGLDDQKINALLMELFGAGTETSASTIEWAIAELTKNPRITSKLQSELLNVVGENTVKE 403
           ++GL+DQKINALLMELFG GTETSASTIEWAI ELTK P + SK++ EL+NVVG+NTVKE
Sbjct: 271 NAGLEDQKINALLMELFGTGTETSASTIEWAITELTKKPLVVSKIRLELVNVVGDNTVKE 330

Query: 404 SDIPHLPYLQAFVK 445
           SD+PHLPYLQAFVK
Sbjct: 331 SDLPHLPYLQAFVK 344


>gb|AAU20767.1| (S)-N-methylcoclaurine 3'-hydroxylase [Thalictrum flavum subsp.
           glaucum]
          Length = 491

 Score =  209 bits (533), Expect = 1e-52
 Identities = 105/148 (70%), Positives = 117/148 (79%)
 Frame = +2

Query: 2   GSAXXXXXXXXXXXXGNSTNLADYFPVLGSLDLFGQRRAVAEVLKQIYDVWGAMLKERRG 181
           GSA            GNSTN ADYFP++G  DLFGQRR VA+VLKQIYDVWG MLKERR 
Sbjct: 197 GSAELKEHLWKLLDMGNSTNPADYFPIMGKFDLFGQRREVAKVLKQIYDVWGVMLKERRS 256

Query: 182 AKGDCRNDFVNVLIDSGLDDQKINALLMELFGAGTETSASTIEWAIAELTKNPRITSKLQ 361
             G   NDF NVL+++GLDDQKINALLMELFGAGTET+ASTIEWAI ELT N R+ SKL+
Sbjct: 257 ITGHRENDFANVLLNAGLDDQKINALLMELFGAGTETTASTIEWAITELTNNLRVISKLR 316

Query: 362 SELLNVVGENTVKESDIPHLPYLQAFVK 445
           +EL+NVVG  TVKES+IPHLPYLQA VK
Sbjct: 317 AELINVVGHKTVKESNIPHLPYLQAIVK 344


>gb|ACO90224.1| putative (S)-N-methylcoclaurine 3'-hydroxylase [Papaver bracteatum]
          Length = 486

 Score =  196 bits (498), Expect = 2e-48
 Identities = 97/136 (71%), Positives = 116/136 (85%), Gaps = 3/136 (2%)
 Frame = +2

Query: 47  GNSTNLADYFPVLGSLDLFGQRRAVAEVLKQIYDVWGAMLKERRGAK---GDCRNDFVNV 217
           GNSTN ADYFP LG  DLFGQR+ VA+ L+ IY VWGAMLKER+ AK      +NDFV +
Sbjct: 210 GNSTNPADYFPFLGRFDLFGQRKDVADCLQGIYSVWGAMLKERKIAKLHNNSKKNDFVEI 269

Query: 218 LIDSGLDDQKINALLMELFGAGTETSASTIEWAIAELTKNPRITSKLQSELLNVVGENTV 397
           L+DSGLDDQ+INALLME+FGAGTETSASTIEWA++ELTKNP +T+ ++SELL+VVG+  V
Sbjct: 270 LLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPEVTANMRSELLSVVGKRPV 329

Query: 398 KESDIPHLPYLQAFVK 445
           KESDIP++PYLQAFVK
Sbjct: 330 KESDIPNMPYLQAFVK 345


>gb|AAF05621.1|AF191772_1 (S)-N-methylcoclaurine 3'-hydroxylase [Papaver somniferum]
          Length = 481

 Score =  192 bits (488), Expect = 2e-47
 Identities = 95/136 (69%), Positives = 115/136 (84%), Gaps = 3/136 (2%)
 Frame = +2

Query: 47  GNSTNLADYFPVLGSLDLFGQRRAVAEVLKQIYDVWGAMLKERRGAK---GDCRNDFVNV 217
           GNSTN ADYFP LG  DLFGQ + VA+ L+ IY VWGAMLKER+ AK      +NDFV +
Sbjct: 205 GNSTNPADYFPFLGKFDLFGQSKDVADCLQGIYSVWGAMLKERKIAKQHNNSKKNDFVEI 264

Query: 218 LIDSGLDDQKINALLMELFGAGTETSASTIEWAIAELTKNPRITSKLQSELLNVVGENTV 397
           L+DSGLDDQ+INALLME+FGAGTETSASTIEWA++ELTKNP++T+ ++ ELL+VVG+  V
Sbjct: 265 LLDSGLDDQQINALLMEIFGAGTETSASTIEWALSELTKNPQVTANMRLELLSVVGKRPV 324

Query: 398 KESDIPHLPYLQAFVK 445
           KESDIP++PYLQAFVK
Sbjct: 325 KESDIPNMPYLQAFVK 340