BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Aconitum21_contig00015661
         (1002 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|NP_001242478.1| uncharacterized protein LOC100811411 [Glycin...   414   e-113
sp|Q3L181.1|PERR_RAUSE RecName: Full=Perakine reductase gi|59896...   412   e-113
pdb|3UYI|A Chain A, Crystal Structure Of Perakine Reductase, Fou...   412   e-113
pdb|3V0U|A Chain A, Crystal Structure Of Perakine Reductase, Fou...   411   e-112
pdb|3V0T|A Chain A, Crystal Structure Of Perakine Reductase, Fou...   411   e-112

>ref|NP_001242478.1| uncharacterized protein LOC100811411 [Glycine max]
           gi|255637199|gb|ACU18930.1| unknown [Glycine max]
          Length = 348

 Score =  414 bits (1065), Expect = e-113
 Identities = 192/297 (64%), Positives = 242/297 (81%), Gaps = 3/297 (1%)
 Frame = +3

Query: 3   IEDAFNKGITFFDTSDFYGSNHDNEFLIGKVLKRLPREKIQLATKFGVS---PVGTTIKV 173
           I++ FNKG+TFFDTSD YG NHDNE ++GK LK+LPREK+QLATKFGV+   P G    V
Sbjct: 47  IKEVFNKGVTFFDTSDLYGQNHDNEIMVGKALKQLPREKVQLATKFGVTVSGPDGLDFGV 106

Query: 174 CGKPEYVRKCCEESLKRLGIDYIDLYYVHRVDKTVPIEATMGXXXXXXXXXXXXYIGLSE 353
            G PEYVR+CCE SLKRL +D+IDLYY HRVD +VPIE TMG            YIGLSE
Sbjct: 107 KGTPEYVRQCCEASLKRLDVDHIDLYYQHRVDTSVPIEDTMGELKQFVNEGKIKYIGLSE 166

Query: 354 ASSDTIRRAHAVHPITAVQLEWSLWSRDIEEQIVPLCRELGIGIVAYSPIGAGFFGGRAV 533
           A++D IRRAHAVHPITA+Q+E+SLW+RDIEE+I+PLCR+LGIGIVAYSP+G GFF G+AV
Sbjct: 167 ANADAIRRAHAVHPITALQMEYSLWTRDIEEEIIPLCRQLGIGIVAYSPLGRGFFAGKAV 226

Query: 534 TESVPANSILAIHPRFTGENLEKNKVLYERVANLAAKHGCTPSQLALSWVLHQGEDVVPI 713
            E++P+ S+L++HPRFTGENLEKNK+ Y+R+ +LA+KH CTPSQLAL+W+LHQG D++PI
Sbjct: 227 VETLPSQSLLSMHPRFTGENLEKNKLFYKRLDDLASKHACTPSQLALAWLLHQGNDIIPI 286

Query: 714 PGTTKIKNLDNNIGAMELKLTENDLKEITAAVPLDEVAGVGHYEHIYPFSWKFTNTP 884
           PGTTK+KN +NNIG++ +KLTE DL+E++ AVP+ EVAG   Y  +  ++WKF  TP
Sbjct: 287 PGTTKLKNFENNIGSLTVKLTEEDLRELSEAVPVYEVAGTREYGMLSNYTWKFATTP 343


>sp|Q3L181.1|PERR_RAUSE RecName: Full=Perakine reductase gi|59896631|gb|AAX11684.1|
           perakine reductase [Rauvolfia serpentina]
          Length = 337

 Score =  412 bits (1059), Expect = e-113
 Identities = 197/295 (66%), Positives = 236/295 (80%), Gaps = 1/295 (0%)
 Frame = +3

Query: 3   IEDAFNKGITFFDTSDFYGSNHDNEFLIGKVLKRLPREKIQLATKFGVSPVGTT-IKVCG 179
           I++AFN GITFFDTSD YG N  NE L+GK LK+LPREKIQ+ TKFG+  +G + +K  G
Sbjct: 40  IKEAFNCGITFFDTSDIYGENGSNEELLGKALKQLPREKIQVGTKFGIHEIGFSGVKAKG 99

Query: 180 KPEYVRKCCEESLKRLGIDYIDLYYVHRVDKTVPIEATMGXXXXXXXXXXXXYIGLSEAS 359
            P+YVR CCE SLKRL +DYIDL+Y+HR+D TVPIE TMG            Y+GLSEAS
Sbjct: 100 TPDYVRSCCEASLKRLDVDYIDLFYIHRIDTTVPIEITMGELKKLVEEGKIKYVGLSEAS 159

Query: 360 SDTIRRAHAVHPITAVQLEWSLWSRDIEEQIVPLCRELGIGIVAYSPIGAGFFGGRAVTE 539
            DTIRRAHAVHP+TA+Q+E+SLW+RDIE++IVPLCR+LGIGIV YSPIG G F G+A+ E
Sbjct: 160 PDTIRRAHAVHPVTALQIEYSLWTRDIEDEIVPLCRQLGIGIVPYSPIGRGLFAGKAIKE 219

Query: 540 SVPANSILAIHPRFTGENLEKNKVLYERVANLAAKHGCTPSQLALSWVLHQGEDVVPIPG 719
           S+P NS+L  HPRF GENLEKNK +Y R+  L+ KHGCTP QLAL+WVLHQGEDVVPIPG
Sbjct: 220 SLPENSVLTSHPRFVGENLEKNKQIYYRIEALSQKHGCTPVQLALAWVLHQGEDVVPIPG 279

Query: 720 TTKIKNLDNNIGAMELKLTENDLKEITAAVPLDEVAGVGHYEHIYPFSWKFTNTP 884
           TTKIKNL NN+GA+++KLT+ DLKEI+ AVPLDEVAG   +E I   +WKF NTP
Sbjct: 280 TTKIKNLHNNVGALKVKLTKEDLKEISDAVPLDEVAGESIHEVIAVTNWKFANTP 334


>pdb|3UYI|A Chain A, Crystal Structure Of Perakine Reductase, Founder Member Of
           A Novel Akr Subfamily With Unique Conformational Changes
           During Nadph Binding
          Length = 337

 Score =  412 bits (1059), Expect = e-113
 Identities = 198/295 (67%), Positives = 237/295 (80%), Gaps = 1/295 (0%)
 Frame = +3

Query: 3   IEDAFNKGITFFDTSDFYGSNHDNEFLIGKVLKRLPREKIQLATKFGVSPVGTT-IKVCG 179
           I++AFN GITFFDTSD YG N  NE L+GK LK+LPREKIQ+ TKFG+  +G + +K  G
Sbjct: 41  IKEAFNCGITFFDTSDIYGENGSNEELLGKALKQLPREKIQVGTKFGIHEIGFSGVKAKG 100

Query: 180 KPEYVRKCCEESLKRLGIDYIDLYYVHRVDKTVPIEATMGXXXXXXXXXXXXYIGLSEAS 359
            P+YVR CCE SLKRL +DYIDL+Y+HR+D TVPIE TMG            Y+GLSEAS
Sbjct: 101 TPDYVRSCCEASLKRLDVDYIDLFYIHRIDTTVPIEITMGELXKLVEEGKIKYVGLSEAS 160

Query: 360 SDTIRRAHAVHPITAVQLEWSLWSRDIEEQIVPLCRELGIGIVAYSPIGAGFFGGRAVTE 539
            DTIRRAHAVHP+TA+Q+E+SLW+RDIE++IVPLCR+LGIGIV YSPIG G F G+A+ E
Sbjct: 161 PDTIRRAHAVHPVTALQIEYSLWTRDIEDEIVPLCRQLGIGIVPYSPIGRGLFAGKAIKE 220

Query: 540 SVPANSILAIHPRFTGENLEKNKVLYERVANLAAKHGCTPSQLALSWVLHQGEDVVPIPG 719
           S+P NS+L  HPRF GENLEKNK +Y R+  L+ KHGCTP QLAL+WVLHQGEDVVPIPG
Sbjct: 221 SLPENSVLTSHPRFVGENLEKNKQIYYRIEALSQKHGCTPVQLALAWVLHQGEDVVPIPG 280

Query: 720 TTKIKNLDNNIGAMELKLTENDLKEITAAVPLDEVAGVGHYEHIYPFSWKFTNTP 884
           TTKIKNL NN+GA+++KLT+ DLKEI+ AVPLDEVAG   +E I   +WKF NTP
Sbjct: 281 TTKIKNLHNNVGALKVKLTKEDLKEISDAVPLDEVAGESIHEVIAVTNWKFANTP 335


>pdb|3V0U|A Chain A, Crystal Structure Of Perakine Reductase, Founder Member Of
           A Novel Akr Subfamily With Unique Conformational Changes
           During Nadph Binding
          Length = 338

 Score =  411 bits (1057), Expect = e-112
 Identities = 197/295 (66%), Positives = 236/295 (80%), Gaps = 1/295 (0%)
 Frame = +3

Query: 3   IEDAFNKGITFFDTSDFYGSNHDNEFLIGKVLKRLPREKIQLATKFGVSPVGTT-IKVCG 179
           I++AFN GITFFDTSD YG N  NE L+GK LK+LPREKIQ+ TKFG+  +G + +K  G
Sbjct: 41  IKEAFNCGITFFDTSDIYGENGSNEELLGKALKQLPREKIQVGTKFGIHEIGFSGVKAKG 100

Query: 180 KPEYVRKCCEESLKRLGIDYIDLYYVHRVDKTVPIEATMGXXXXXXXXXXXXYIGLSEAS 359
            P+YVR CCE SLKRL +DYIDL+Y+HR+D TVPIE TMG            Y+GLSEAS
Sbjct: 101 TPDYVRSCCEASLKRLDVDYIDLFYIHRIDTTVPIEITMGELKKLVEEGKIKYVGLSEAS 160

Query: 360 SDTIRRAHAVHPITAVQLEWSLWSRDIEEQIVPLCRELGIGIVAYSPIGAGFFGGRAVTE 539
            DTIRRAHAVHP+TA+Q+E+SLW+RDIE++IVPLCR+LGIGIV YSPIG G F G+A+ E
Sbjct: 161 PDTIRRAHAVHPVTALQIEYSLWTRDIEDEIVPLCRQLGIGIVPYSPIGRGLFWGKAIKE 220

Query: 540 SVPANSILAIHPRFTGENLEKNKVLYERVANLAAKHGCTPSQLALSWVLHQGEDVVPIPG 719
           S+P NS+L  HPRF GENLEKNK +Y R+  L+ KHGCTP QLAL+WVLHQGEDVVPIPG
Sbjct: 221 SLPENSVLTSHPRFVGENLEKNKQIYYRIEALSQKHGCTPVQLALAWVLHQGEDVVPIPG 280

Query: 720 TTKIKNLDNNIGAMELKLTENDLKEITAAVPLDEVAGVGHYEHIYPFSWKFTNTP 884
           TTKIKNL NN+GA+++KLT+ DLKEI+ AVPLDEVAG   +E I   +WKF NTP
Sbjct: 281 TTKIKNLHNNVGALKVKLTKEDLKEISDAVPLDEVAGESIHEVIAVTNWKFANTP 335


>pdb|3V0T|A Chain A, Crystal Structure Of Perakine Reductase, Founder Member Of
           A Novel Akr Subfamily With Unique Conformational Changes
           During Nadph Binding
          Length = 337

 Score =  411 bits (1057), Expect = e-112
 Identities = 197/295 (66%), Positives = 236/295 (80%), Gaps = 1/295 (0%)
 Frame = +3

Query: 3   IEDAFNKGITFFDTSDFYGSNHDNEFLIGKVLKRLPREKIQLATKFGVSPVGTT-IKVCG 179
           I++AFN GITFFDTSD YG N  NE L+GK LK+LPREKIQ+ TKFG+  +G + +K  G
Sbjct: 40  IKEAFNCGITFFDTSDIYGENGSNEELLGKALKQLPREKIQVGTKFGIHEIGFSGVKAKG 99

Query: 180 KPEYVRKCCEESLKRLGIDYIDLYYVHRVDKTVPIEATMGXXXXXXXXXXXXYIGLSEAS 359
            P+YVR CCE SLKRL +DYIDL+Y+HR+D TVPIE TMG            Y+GLSEAS
Sbjct: 100 TPDYVRSCCEASLKRLDVDYIDLFYIHRIDTTVPIEITMGELKKLVEEGKIKYVGLSEAS 159

Query: 360 SDTIRRAHAVHPITAVQLEWSLWSRDIEEQIVPLCRELGIGIVAYSPIGAGFFGGRAVTE 539
            DTIRRAHAVHP+TA+Q+E+SLW+RDIE++IVPLCR+LGIGIV YSPIG G F G+A+ E
Sbjct: 160 PDTIRRAHAVHPVTALQIEYSLWTRDIEDEIVPLCRQLGIGIVPYSPIGRGLFWGKAIKE 219

Query: 540 SVPANSILAIHPRFTGENLEKNKVLYERVANLAAKHGCTPSQLALSWVLHQGEDVVPIPG 719
           S+P NS+L  HPRF GENLEKNK +Y R+  L+ KHGCTP QLAL+WVLHQGEDVVPIPG
Sbjct: 220 SLPENSVLTSHPRFVGENLEKNKQIYYRIEALSQKHGCTPVQLALAWVLHQGEDVVPIPG 279

Query: 720 TTKIKNLDNNIGAMELKLTENDLKEITAAVPLDEVAGVGHYEHIYPFSWKFTNTP 884
           TTKIKNL NN+GA+++KLT+ DLKEI+ AVPLDEVAG   +E I   +WKF NTP
Sbjct: 280 TTKIKNLHNNVGALKVKLTKEDLKEISDAVPLDEVAGESIHEVIAVTNWKFANTP 334