BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Aconitum21_contig00010136
         (1012 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-meth...   414   e-113
gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictr...   411   e-112
gb|ACO90250.1| norcoclaurine 6-O-methyltransferase-like protein ...   370   e-100
dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica]        335   1e-89
gb|AAQ01669.1| (R,S)-norcoclaurine 6-O-methyltransferase [Papave...   317   2e-84

>sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-methyltransferase; AltName:
           Full=S-adenosyl-L-methionine:norcoclaurine
           6-O-methyltransferase; Short=6-OMT
           gi|9711914|dbj|BAB08004.1|
           S-adenosyl-L-methionine:norcoclaurine
           6-O-methyltransferase [Coptis japonica]
          Length = 347

 Score =  414 bits (1065), Expect = e-113
 Identities = 199/293 (67%), Positives = 233/293 (79%), Gaps = 1/293 (0%)
 Frame = +3

Query: 6   LPNQPVNENALFRIMRYLVHMKILTQSLVAGETRYGLAPPAKYLVKGWDKCMVTEILFYS 185
           LP+QPVNE+AL+R+MRYLVHMK+ T++ + GE RYGLAPPAKYLVKGWDKCMV  IL  +
Sbjct: 55  LPSQPVNEDALYRVMRYLVHMKLFTKASIDGELRYGLAPPAKYLVKGWDKCMVGSILAIT 114

Query: 186 DKDLMASWEFLNENVSNENGKAYEKALGMDSWGYLEANPEKAQLFNEAMANDTRLTMSAL 365
           DKD MA W +L + +S E+G A+EKALG + WGY+  +PEK QLFNEAMAND+RL MSAL
Sbjct: 115 DKDFMAPWHYLKDGLSGESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLIMSAL 174

Query: 366 VRECSGSFEXXXXXXXXXXXXXXAAMNIAKAFPHIKCTVYDLPRVIAASPSNSEVNFVGG 545
           V+EC   F               A  NIA AFPHIKCTVYDLP VIA SP  SEV+ V G
Sbjct: 175 VKECGNIFNGITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAG 234

Query: 546 DMFQSVPKADAIMMKCVLHDWDDMQCVKILKKCKEAVPREGGKVIIVDVVLNTESEHPYT 725
           DMF+ +PKADAIMMKC+LHDWDD +C++ILK+CKEAVP +GGKVIIVD+VLN +SEHPYT
Sbjct: 235 DMFKFIPKADAIMMKCILHDWDDKECIEILKRCKEAVPVKGGKVIIVDIVLNVQSEHPYT 294

Query: 726 KTRLTFDLHMM-PFSGKERTEDEWKKLIHDAGYRGYKITHISAVQSVIEAYPY 881
           K RLT DL MM    GKERTE+EWKKLIHDAGY+G+KIT I+AVQSVIEAYPY
Sbjct: 295 KMRLTLDLDMMLNTGGKERTEEEWKKLIHDAGYKGHKITQITAVQSVIEAYPY 347


>gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictrum flavum subsp.
           glaucum]
          Length = 350

 Score =  411 bits (1056), Expect = e-112
 Identities = 194/296 (65%), Positives = 234/296 (79%), Gaps = 3/296 (1%)
 Frame = +3

Query: 3   HLPNQPVNENALFRIMRYLVHMKILTQSLVAGETRYGLAPPAKYLVKGWDKCMVTEILFY 182
           HLP+QPVN++AL+R++RYLVHMK+ T+S + GE RYGLAPPAK+LVKGWDKCM+  IL  
Sbjct: 55  HLPSQPVNQDALYRVLRYLVHMKLFTKSSIDGELRYGLAPPAKFLVKGWDKCMLGAILTI 114

Query: 183 SDKDLMASWEFLNENVSNENGK--AYEKALGMDSWGYLEANPEKAQLFNEAMANDTRLTM 356
           +DKD MA W +L E + N+     A+EKALG + W Y+  +PEK QLFNE MANDTRL M
Sbjct: 115 TDKDFMAPWHYLKEGILNDGSTSTAFEKALGTNIWDYMAEHPEKNQLFNEGMANDTRLIM 174

Query: 357 SALVRECSGSFEXXXXXXXXXXXXXXAAMNIAKAFPHIKCTVYDLPRVIAASPSNSEVNF 536
           SALV+ECS  F+              A  NIAKAFPHIKCTVYDLP VIA SP  +E+N 
Sbjct: 175 SALVKECSSMFDGITTIVDVGGGTGTAVRNIAKAFPHIKCTVYDLPHVIADSPGYTEINS 234

Query: 537 VGGDMFQSVPKADAIMMKCVLHDWDDMQCVKILKKCKEAVPREGGKVIIVDVVLNTESEH 716
           + GDMF+ +P ADAIMMKC+LHDWDD +C++ILK+CK+AVPR+GGKVII+D++L+ +SEH
Sbjct: 235 IQGDMFKYIPNADAIMMKCILHDWDDKECIEILKRCKDAVPRDGGKVIIIDIILDVKSEH 294

Query: 717 PYTKTRLTFDLHMM-PFSGKERTEDEWKKLIHDAGYRGYKITHISAVQSVIEAYPY 881
           PYTK RLT DL MM    GKERTE+EWKKLIHDAGY+GYKITHISAVQSVIEAYPY
Sbjct: 295 PYTKMRLTLDLDMMLNTGGKERTEEEWKKLIHDAGYKGYKITHISAVQSVIEAYPY 350


>gb|ACO90250.1| norcoclaurine 6-O-methyltransferase-like protein [Thalictrum
           flavum]
          Length = 346

 Score =  370 bits (951), Expect = e-100
 Identities = 180/296 (60%), Positives = 227/296 (76%), Gaps = 4/296 (1%)
 Frame = +3

Query: 6   LPNQPVNENALFRIMRYLVHMKILTQSLVAGETRYGLAPPAKYLVKGWDKCMVTEILFYS 185
           LP+QPVN++AL+R+MRYLVH+K+ T+S + GE RYGLAPPAK+LVKGWD CM+  IL   
Sbjct: 51  LPSQPVNQDALYRVMRYLVHIKLFTKSSIDGELRYGLAPPAKFLVKGWDNCMIGSILLMV 110

Query: 186 DKDLMASWEFLNENVSNENGK--AYEKALGMDSWGYLEANPEKAQLFNEAMANDTRLTMS 359
           D D MA + ++ + + N+     A+EKA G + W YL  +PEK QLFNE MAN++RLTMS
Sbjct: 111 DTDFMAPYHYIKDEILNDGSSITAFEKASGTNIWEYLAEHPEKNQLFNEGMANESRLTMS 170

Query: 360 ALVRECSGSFEXXXXXXXXXXXXXXAAMNIAKAFPHIKCTVYDLPRVIAASPSNSEVNFV 539
            L+++CS +FE                  IAKAFP+IK TVYDLP VIA +P ++E+NFV
Sbjct: 171 GLMKDCSSTFEGLTTLVDVGGGTGTTVKYIAKAFPNIKYTVYDLPHVIADAPGDTEINFV 230

Query: 540 GGDMFQSVPKADAIMMKCVLHDWDDMQCVKILKKCKEAVPREGGKVIIVDVVLNTESEHP 719
            GDMF+ +P ADAI+MK VLHDWDD +C++ILK+CK+AVPR+GGKVII+DVVLN +SEHP
Sbjct: 231 EGDMFKYIPNADAILMKGVLHDWDDNECIEILKRCKDAVPRDGGKVIIIDVVLNVKSEHP 290

Query: 720 YTKTRLTFDLHMM-PFSGKERTEDEWKKLIH-DAGYRGYKITHISAVQSVIEAYPY 881
           YTK RLT DL MM    GKERTE++WKKLI+ DAGY+GYKIT ISA+QSVIEAYPY
Sbjct: 291 YTKMRLTLDLDMMLNNKGKERTEEDWKKLIYDDAGYKGYKITQISALQSVIEAYPY 346


>dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica]
          Length = 351

 Score =  335 bits (858), Expect = 1e-89
 Identities = 164/295 (55%), Positives = 211/295 (71%), Gaps = 3/295 (1%)
 Frame = +3

Query: 6   LPNQPVNENALFRIMRYLVHMKILTQSLVAGET--RYGLAPPAKYLVKGWDKCMVTEILF 179
           LP QPV+ + L+R+MRYLVHMK+  +     E   +Y L PPAK+L+KGW+K MV+ IL 
Sbjct: 58  LPVQPVDLDRLYRVMRYLVHMKLFKKQEDEDEESIKYSLLPPAKFLIKGWEKSMVSAILL 117

Query: 180 YSDKDLMASWEFLNENVSNENGKAYEKALGMDSWGYLEANPEKAQLFNEAMANDTRLTMS 359
            +DKD M+ W  L E + N N  A++KALG   W Y+ ANP+K ++FNE MA D+RL  S
Sbjct: 118 INDKDFMSPWHHLKEGLVN-NSNAFDKALGKSIWDYMSANPDKNKVFNEGMACDSRLVSS 176

Query: 360 ALVRECSGSFEXXXXXXXXXXXXXXAAMNIAKAFPHIKCTVYDLPRVIAASPSNSEVNFV 539
           ALV EC   F+              A   ++KAFPHIKC++YDLP VI+ SP    +  +
Sbjct: 177 ALVNECGNIFQGIQTLVDVGGGTGTAVKAVSKAFPHIKCSIYDLPHVISDSPDIPNIVKI 236

Query: 540 GGDMFQSVPKADAIMMKCVLHDWDDMQCVKILKKCKEAVPREGGKVIIVDVVLNTESEHP 719
            G+MF+S+P ADAI+MKC+LHDW+D +C++ILKKCKEAVP+EGGKVIIVD+VL+ ES+HP
Sbjct: 237 EGEMFKSIPSADAILMKCILHDWNDDECIEILKKCKEAVPKEGGKVIIVDIVLDMESKHP 296

Query: 720 YTKTRLTFDLHMM-PFSGKERTEDEWKKLIHDAGYRGYKITHISAVQSVIEAYPY 881
           Y+K RLT DL MM    GKERTE+EWKKLI  AG+  YKIT ISA+QSVIEA+PY
Sbjct: 297 YSKVRLTLDLDMMLNTEGKERTEEEWKKLIDGAGFASYKITQISALQSVIEAFPY 351


>gb|AAQ01669.1| (R,S)-norcoclaurine 6-O-methyltransferase [Papaver somniferum]
          Length = 346

 Score =  317 bits (813), Expect = 2e-84
 Identities = 163/291 (56%), Positives = 201/291 (69%), Gaps = 2/291 (0%)
 Frame = +3

Query: 15  QPVNENALFRIMRYLVHMKILTQSLVAGETRYGLAPPAKYLVKGWDKCMVTEILFYSDKD 194
           QPVNE+ LFRIMRYLVHM++    + A   +Y LAPPAKYL++GW+K MV  IL  +DKD
Sbjct: 60  QPVNEDRLFRIMRYLVHMELF--KIDATTQKYSLAPPAKYLLRGWEKSMVDSILCINDKD 117

Query: 195 LMASWEFLNENVSNENGKAYEKALGMDSWGYLEANPEKAQLFNEAMANDTRLTMSALVRE 374
            +A W  L + ++  N  A+EKALG   W Y+  NPEK QLFN AMA DTRL  SAL  E
Sbjct: 118 FLAPWHHLGDGLTG-NCDAFEKALGKSIWVYMSVNPEKNQLFNAAMACDTRLVTSALANE 176

Query: 375 CSGSFEXXXXXXXXXXXXXXAAMN-IAKAFPHIKCTVYDLPRVIAASPSNSEVNFVGGDM 551
           C   F                A+  I+KAFP IKCT+YDLP VIA SP    +  + GDM
Sbjct: 177 CKSIFSDGISTLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDM 236

Query: 552 FQSVPKADAIMMKCVLHDWDDMQCVKILKKCKEAVPREGGKVIIVDVVLNTESEHPYTKT 731
           F+S+P ADAI MKC+LHDW+D +C++ILK+CKEA+P+ GGKVIIVDVV++ +S HPY K 
Sbjct: 237 FKSIPSADAIFMKCILHDWNDDECIQILKRCKEALPK-GGKVIIVDVVIDMDSTHPYAKI 295

Query: 732 RLTFDLHMM-PFSGKERTEDEWKKLIHDAGYRGYKITHISAVQSVIEAYPY 881
           RLT DL MM    GKERT++EWK L   AG+  +K+T ISAVQSVIEAYPY
Sbjct: 296 RLTLDLDMMLNTGGKERTKEEWKTLFDAAGFASHKVTQISAVQSVIEAYPY 346