BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Aconitum21_contig00005425
         (1625 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. diss...   741   0.0  
sp|P47195.1|C80A1_BERST RecName: Full=Berbamunine synthase; AltN...   500   e-139
gb|AGC29949.1| CYP80G3 [Sinopodophyllum hexandrum]                    486   e-135
gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chi...   462   e-127
sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcocla...   461   e-127

>dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. dissecta]
          Length = 486

 Score =  741 bits (1912), Expect = 0.0
 Identities = 365/488 (74%), Positives = 417/488 (85%)
 Frame = -1

Query: 1607 MDIQIATYCIIFAILVMLALASKTKHKNLPPGPSAWPLIGSLPMLFTNTQVPLHVTLTNL 1428
            MD+QIA + +I  ILV + L  K K+KNLPPGP  WPLIG+LP+LFTNT+VPLH+TL N+
Sbjct: 1    MDLQIALFSLIPVILVFILLL-KPKYKNLPPGPHPWPLIGNLPILFTNTEVPLHITLANM 59

Query: 1427 AAAHGPIMTLWLGTQPTVIASTPESAAEVLKTNDRVCSGRYIPMSFRLKHHIKHSLVWAD 1248
            A  HGPIM LWLGTQPTV+AST E+A E+LKT+DR+ S R+I MSFRLKHHIK+SLVW+D
Sbjct: 60   ARTHGPIMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHIRMSFRLKHHIKYSLVWSD 119

Query: 1247 CTDNWKLLRKIARTEIFSPKMLQTQSRVREEKAGELVDFLRSKQGQAVKLSTFVFGTLLN 1068
            CTD WKLLRKI RTEIFSPKMLQ QS VRE+K  EL+DFLRSK+GQ VK+S FVFGTLLN
Sbjct: 120  CTDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVKISQFVFGTLLN 179

Query: 1067 ILGNAVYSEDVFVYSDDEGGKQGIQSMIKEMLMIGAEPNLAEFYPILEGLDLQGLKRKCD 888
            ILGN V+S+DVFVY D E  K GIQ++I+EMLMIGAEPN+AEFYP LE LDLQGLK+KCD
Sbjct: 180  ILGNVVFSKDVFVYGD-ETDKGGIQNLIREMLMIGAEPNVAEFYPSLEELDLQGLKKKCD 238

Query: 887  DRFVRVMKLWEGTVXXXXXXXXXXXXKDMLDVLLANDFNDAQINALFLETFGPGSETSSA 708
            +RF+RVMK+WEGTV             DMLDVLLANDFNDAQINALFLETFGPGSETSSA
Sbjct: 239  ERFIRVMKMWEGTVKERKANRNEESK-DMLDVLLANDFNDAQINALFLETFGPGSETSSA 297

Query: 707  TIEWVMAELIKNPDKMAKVREELVQVVGTSTVKESHLPQLPYLHACIKEAMRLHPAAPFL 528
            TIEWV+AELIK+P +MAKVR+EL +VVGTST+KES LPQLPYL ACIKEAMRLHPAAPFL
Sbjct: 298  TIEWVIAELIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACIKEAMRLHPAAPFL 357

Query: 527  LSHRAVESCKIMGYTIPKDCKILVNAYAIGRDPKSWNDPTTFRPERFLESDVDYHGGHYK 348
            L  RA E+C++MGYTIPK+ ++LVNAYAIGRDPKSW DP+TF PERFLESDVD+HG HY+
Sbjct: 358  LPRRAAETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERFLESDVDFHGAHYQ 417

Query: 347  FIPFGSGRRICIGMPLATRTIPLIVASLVHAYDWRLPNRKHPQDLEMKEMLSLSLAIDPS 168
            FIPFGSGRR C+GMPLATRTIPLIV SLVH YD+ LP    P+DL+M EMLSL+LAIDPS
Sbjct: 418  FIPFGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPEDLKMNEMLSLTLAIDPS 477

Query: 167  LCVVPNLR 144
            LCVVP  R
Sbjct: 478  LCVVPKAR 485


>sp|P47195.1|C80A1_BERST RecName: Full=Berbamunine synthase; AltName:
            Full=(S)-N-methylcoclaurine oxidase [C-O
            phenol-coupling]; AltName: Full=CYPLXXX; AltName:
            Full=Cytochrome P450 80 gi|642386|gb|AAC48987.1|
            cytochrome P-450 CYP80 [Berberis stolonifera]
          Length = 487

 Score =  500 bits (1288), Expect = e-139
 Identities = 247/485 (50%), Positives = 339/485 (69%), Gaps = 1/485 (0%)
 Frame = -1

Query: 1595 IATYCIIFAILVMLALASKTKHKNLPPGPSAWPLIGSLPMLFTNTQVPLHVTLTNLAAAH 1416
            I  +  I  + ++  L  K KH NLPP P AWP++G LP L +    P    ++N+A  +
Sbjct: 4    IVGFVSISLVALLYFLLFKPKHTNLPPSPPAWPIVGHLPDLISKNSPPFLDYMSNIAQKY 63

Query: 1415 GPIMTLWLGTQPTVIASTPESAAEVLKTNDRVCSGRYIPMSFRLKHHIKHSLVWADCTDN 1236
            GP++ L  G   ++ AST E+A EVL+TND+V SGR     FR+K HI +S++W+D    
Sbjct: 64   GPLIHLKFGLHSSIFASTKEAAMEVLQTNDKVLSGRQPLPCFRIKPHIDYSILWSDSNSY 123

Query: 1235 WKLLRKIARTEIFSPKMLQTQSRVREEKAGELVDFLRSKQGQAVKLSTFVFGTLLNILGN 1056
            WK  RKI  TEIFS KMLQ Q + RE  AG LV+F+ +K G  V+L +++FG  LN+LG+
Sbjct: 124  WKKGRKILHTEIFSQKMLQAQEKNRERVAGNLVNFIMTKVGDVVELRSWLFGCALNVLGH 183

Query: 1055 AVYSEDVFVYSDDEGGKQGIQSMIKEMLMIGAEPNLAEFYPILEGLDLQGLKRKCDDRFV 876
             V+S+DVF YSD +  + G+  +I  MLM G + ++A ++P+L   DL GLKRK D++F 
Sbjct: 184  VVFSKDVFEYSD-QSDEVGMDKLIHGMLMTGGDFDVASYFPVLARFDLHGLKRKMDEQFK 242

Query: 875  RVMKLWEGTVXXXXXXXXXXXXKDMLDVLLANDFNDAQINALFLETFGPGSETSSATIEW 696
             ++K+WEG V             DMLDVL+ANDFN+ QINA+F+ETFGPGS+T+S  IEW
Sbjct: 243  LLIKIWEGEVLARRANRNPEPK-DMLDVLIANDFNEHQINAMFMETFGPGSDTNSNIIEW 301

Query: 695  VMAELIKNPDKMAKVREELVQVVG-TSTVKESHLPQLPYLHACIKEAMRLHPAAPFLLSH 519
             +A+LIKNPDK+AK+REEL +VVG +STVKESH  +LPYL AC+KE MRL+P    ++ H
Sbjct: 302  ALAQLIKNPDKLAKLREELDRVVGRSSTVKESHFSELPYLQACVKETMRLYPPISIMIPH 361

Query: 518  RAVESCKIMGYTIPKDCKILVNAYAIGRDPKSWNDPTTFRPERFLESDVDYHGGHYKFIP 339
            R +E+C++MGYTIPK   + VNA+AIGRDPK W DP  F+PERFL+SD++Y+G  ++FIP
Sbjct: 362  RCMETCQVMGYTIPKGMDVHVNAHAIGRDPKDWKDPLKFQPERFLDSDIEYNGKQFQFIP 421

Query: 338  FGSGRRICIGMPLATRTIPLIVASLVHAYDWRLPNRKHPQDLEMKEMLSLSLAIDPSLCV 159
            FGSGRRIC G PLA R IPL++ASLVHA+ W LP+    + L+M+E+ +LSL +   L V
Sbjct: 422  FGSGRRICPGRPLAVRIIPLVLASLVHAFGWELPDGVPNEKLDMEELFTLSLCMAKPLRV 481

Query: 158  VPNLR 144
            +P +R
Sbjct: 482  IPKVR 486


>gb|AGC29949.1| CYP80G3 [Sinopodophyllum hexandrum]
          Length = 346

 Score =  486 bits (1251), Expect = e-135
 Identities = 241/348 (69%), Positives = 281/348 (80%)
 Frame = -1

Query: 1187 MLQTQSRVREEKAGELVDFLRSKQGQAVKLSTFVFGTLLNILGNAVYSEDVFVYSDDEGG 1008
            MLQ Q  VRE+K  ELV+FL+ K+GQ VK+S  VFGTLLNILGN ++S+DVF Y   +G 
Sbjct: 1    MLQVQEHVREQKVAELVEFLKGKEGQVVKISQLVFGTLLNILGNVIFSKDVFRYVGADGD 60

Query: 1007 KQGIQSMIKEMLMIGAEPNLAEFYPILEGLDLQGLKRKCDDRFVRVMKLWEGTVXXXXXX 828
            K  +Q++IKEMLMIGA PNLAEF+PI E  DLQGLK++C+ RF  VMK+W+GTV      
Sbjct: 61   K--MQTLIKEMLMIGAAPNLAEFWPIFEEFDLQGLKKRCNQRFSEVMKMWKGTVEERKVK 118

Query: 827  XXXXXXKDMLDVLLANDFNDAQINALFLETFGPGSETSSATIEWVMAELIKNPDKMAKVR 648
                   DMLDVLL NDFNDAQINA+FLETFGPGSETSSAT EW +AELIKNP  +AKVR
Sbjct: 119  RNEEPK-DMLDVLLLNDFNDAQINAVFLETFGPGSETSSATSEWALAELIKNPHILAKVR 177

Query: 647  EELVQVVGTSTVKESHLPQLPYLHACIKEAMRLHPAAPFLLSHRAVESCKIMGYTIPKDC 468
            +EL QVVG STVKES LP LPYL ACIKE MRLHPAAPFLL HRAVE+C +MGYTIPKD 
Sbjct: 178  QELDQVVGKSTVKESDLPHLPYLQACIKETMRLHPAAPFLLPHRAVETCPVMGYTIPKDT 237

Query: 467  KILVNAYAIGRDPKSWNDPTTFRPERFLESDVDYHGGHYKFIPFGSGRRICIGMPLATRT 288
            ++LVN YAIGRDPK+W +PTTF+PERFLESDVDY+G H++FIPFG+GRR C+GMPLATRT
Sbjct: 238  QVLVNIYAIGRDPKTWKEPTTFKPERFLESDVDYYGKHFEFIPFGAGRRQCVGMPLATRT 297

Query: 287  IPLIVASLVHAYDWRLPNRKHPQDLEMKEMLSLSLAIDPSLCVVPNLR 144
            IPLIV++LV   DW LP+ K PQDL M E LSLSLA+DP+L V+P  R
Sbjct: 298  IPLIVSNLVQTLDWSLPDGKLPQDLVMNESLSLSLALDPTLTVIPKSR 345


>gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
          Length = 488

 Score =  462 bits (1189), Expect = e-127
 Identities = 227/475 (47%), Positives = 317/475 (66%)
 Frame = -1

Query: 1568 ILVMLALASKTKHKNLPPGPSAWPLIGSLPMLFTNTQVPLHVTLTNLAAAHGPIMTLWLG 1389
            +L +  +    + KNLPPGP   P++G+L  L    +         LA  +G + +L LG
Sbjct: 14   LLFLFFILRDPRQKNLPPGPRPLPIVGNLLQLGDKPRAEF----AKLAQKYGQLFSLKLG 69

Query: 1388 TQPTVIASTPESAAEVLKTNDRVCSGRYIPMSFRLKHHIKHSLVWADCTDNWKLLRKIAR 1209
            +Q  V+AS+P +AAEVLKT+D++ SGRY+  SFR+K H+++S+VW++C DNWKLLRK+ R
Sbjct: 70   SQTVVVASSPAAAAEVLKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDNWKLLRKVCR 129

Query: 1208 TEIFSPKMLQTQSRVREEKAGELVDFLRSKQGQAVKLSTFVFGTLLNILGNAVYSEDVFV 1029
            TE+F+PKM+++QS +RE KA E+V FLRSK+G+ VK+   VFGTL+NI GN ++S+DVF 
Sbjct: 130  TELFTPKMIESQSEIREGKAREMVKFLRSKEGEVVKIVEVVFGTLVNIFGNLIFSKDVFD 189

Query: 1028 YSDDEGGKQGIQSMIKEMLMIGAEPNLAEFYPILEGLDLQGLKRKCDDRFVRVMKLWEGT 849
              D  GG   ++  + ++L +G   N A+++PI+   DL G +R   D   ++  +W   
Sbjct: 190  LEDPTGGSVELKEHLWKLLEMGNSTNPADYFPIMGKYDLFGQRRAVADVLQQIYDVWGIN 249

Query: 848  VXXXXXXXXXXXXKDMLDVLLANDFNDAQINALFLETFGPGSETSSATIEWVMAELIKNP 669
            +             D +DVLL     D +INAL +E FG G+ETS++TIEW + EL K P
Sbjct: 250  LKERRGMKGSESNNDFVDVLLNAGLEDQKINALLMELFGTGTETSASTIEWAITELTKKP 309

Query: 668  DKMAKVREELVQVVGTSTVKESHLPQLPYLHACIKEAMRLHPAAPFLLSHRAVESCKIMG 489
              ++K+R ELV VVG +TVKES LP LPYL A +KE +RLHP  P LL  RA+E+C +M 
Sbjct: 310  LVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETLRLHPPTPLLLPRRALETCTVMN 369

Query: 488  YTIPKDCKILVNAYAIGRDPKSWNDPTTFRPERFLESDVDYHGGHYKFIPFGSGRRICIG 309
            YTIPK+C+I+VNA+AIGRDPK+W+DP  F+PERFL SDVDY G  ++ IPFG GRRIC G
Sbjct: 370  YTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPFGGGRRICPG 429

Query: 308  MPLATRTIPLIVASLVHAYDWRLPNRKHPQDLEMKEMLSLSLAIDPSLCVVPNLR 144
            +PLA++ I LIVA+LV  ++W LP      +L M E   L+L  DP L +V   R
Sbjct: 430  LPLASQFISLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIVLKAR 484


>sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
            2; AltName: Full=Cytochrome P450 80B2
            gi|9971208|dbj|BAB12433.1|
            (S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
          Length = 488

 Score =  461 bits (1185), Expect = e-127
 Identities = 225/475 (47%), Positives = 318/475 (66%)
 Frame = -1

Query: 1568 ILVMLALASKTKHKNLPPGPSAWPLIGSLPMLFTNTQVPLHVTLTNLAAAHGPIMTLWLG 1389
            +L +  +   ++ KNLPPGP   P++G+L  L        H     LA  +G + +L LG
Sbjct: 14   LLFLFFILRDSRPKNLPPGPRPSPIVGNLLQLGDKP----HAEFAKLAQKYGELFSLKLG 69

Query: 1388 TQPTVIASTPESAAEVLKTNDRVCSGRYIPMSFRLKHHIKHSLVWADCTDNWKLLRKIAR 1209
            +Q  V+AS+P +AAE+LKT+D++ SGRY+  SFR+K H+++S+VW++C DNWKLLRK+ R
Sbjct: 70   SQTVVVASSPAAAAEILKTHDKILSGRYVFQSFRVKEHVENSIVWSECNDNWKLLRKVCR 129

Query: 1208 TEIFSPKMLQTQSRVREEKAGELVDFLRSKQGQAVKLSTFVFGTLLNILGNAVYSEDVFV 1029
            TE+F+PKM+++QS +RE KA E+V FLR K+G+ VK+   VFGTL+NI GN ++S+DVF 
Sbjct: 130  TELFTPKMIESQSEIREAKAREMVKFLRGKEGEVVKIVEVVFGTLVNIFGNLIFSKDVFD 189

Query: 1028 YSDDEGGKQGIQSMIKEMLMIGAEPNLAEFYPILEGLDLQGLKRKCDDRFVRVMKLWEGT 849
              D  GG   ++  + ++L +G   N A+++PI+  LDL G +R   +   ++  +W   
Sbjct: 190  LEDPTGGSVELKEHLWKLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVLQQIYDVWGVM 249

Query: 848  VXXXXXXXXXXXXKDMLDVLLANDFNDAQINALFLETFGPGSETSSATIEWVMAELIKNP 669
            +             D +DVLL    +D +INAL +E FG G+ETS++TIEW + EL K P
Sbjct: 250  LKERRGTKGSESKNDFVDVLLNAGLDDQKINALLMELFGAGTETSASTIEWAITELTKKP 309

Query: 668  DKMAKVREELVQVVGTSTVKESHLPQLPYLHACIKEAMRLHPAAPFLLSHRAVESCKIMG 489
              ++K+R ELV VVG +TVKES LP LPYL A +KE +RLHP  P LL  RA+E+C +M 
Sbjct: 310  LVVSKIRLELVNVVGDNTVKESDLPHLPYLQAFVKETLRLHPPTPLLLPRRALETCTVMN 369

Query: 488  YTIPKDCKILVNAYAIGRDPKSWNDPTTFRPERFLESDVDYHGGHYKFIPFGSGRRICIG 309
            YTIPK+C+I+VNA+AIGRDPK+W+DP  F+PERFL SDVDY G  ++ IPFG GRRIC G
Sbjct: 370  YTIPKECQIMVNAWAIGRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPFGGGRRICPG 429

Query: 308  MPLATRTIPLIVASLVHAYDWRLPNRKHPQDLEMKEMLSLSLAIDPSLCVVPNLR 144
            +PLA++   LIVA+LV  ++W LP      +L M E   L+L  DP L +V   R
Sbjct: 430  LPLASQFSNLIVATLVQNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIVLKAR 484