BLASTX nr result
ID: Aconitum21_contig00004429
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Aconitum21_contig00004429
(1326 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002268942.2| PREDICTED: 2-isopropylmalate synthase 2, chl... 578 e-162
sp|O04973.1|LEU1A_SOLPN RecName: Full=2-isopropylmalate synthase... 565 e-158
gb|AAW81741.1| Putative 2-isopropylmalate synthase [Brassica ole... 564 e-158
gb|ABA26446.1| 2-isopropylmalate synthase 1 [Brassica insularis] 564 e-158
ref|NP_177544.1| 2-isopropylmalate synthase 2 [Arabidopsis thali... 562 e-158
>ref|XP_002268942.2| PREDICTED: 2-isopropylmalate synthase 2, chloroplastic-like [Vitis
vinifera]
Length = 628
Score = 578 bits (1489), Expect = e-162
Identities = 283/335 (84%), Positives = 313/335 (93%)
Frame = -1
Query: 1326 EAGFPASSKDDLQAVKLIAQEVGNAVDGDGYVPVICGLARCNRSDIDAAWEAVKHAKRPR 1147
EAGFPA+SKDDL AVK+IA+EVGNAVD +G+VPVICGLARCNR+DI AWEAVK+AKRPR
Sbjct: 125 EAGFPAASKDDLDAVKIIAKEVGNAVDENGHVPVICGLARCNRNDISTAWEAVKYAKRPR 184
Query: 1146 IHTFIATSEIHLKYKLKKSRDEVVAIAREMVSYARSLGCNDIEFSPEDAGRSDREYLYEV 967
IHTFIATSEIH+KYKLKKS EV+ IAREMV YARSLGC+D+EFSPEDAGRS+RE+LY++
Sbjct: 185 IHTFIATSEIHMKYKLKKSEKEVIEIAREMVKYARSLGCDDVEFSPEDAGRSEREFLYKI 244
Query: 966 LGEVIKAGATTLNIPDTVGYNLPIEFGKLIADIKSNTPGIENVIISTHCQNDLGLSTANT 787
LGEVIKAGATTLNIPDTVGY +P EFG+LIADIKSNTPGIENVIISTHCQNDLGLSTANT
Sbjct: 245 LGEVIKAGATTLNIPDTVGYTVPNEFGQLIADIKSNTPGIENVIISTHCQNDLGLSTANT 304
Query: 786 IAGAHAGARQLEVTVNGIGERAGNASLEEVVMAIKCRGNELLSGLYTGVNTKHIVMASRM 607
IAGA AGARQ+EVT+NGIGERAGNASLEEVVMA+KCRG + L GLYTG+N +HIV S+M
Sbjct: 305 IAGACAGARQVEVTINGIGERAGNASLEEVVMALKCRGEKALGGLYTGINIRHIVKTSKM 364
Query: 606 VAEYTGLQVQPHKAIVGANAFAHESGIHQDGMLKFKGTYEIMSPEDIGLSRSNDSGIVLG 427
VAEY+GLQVQPHKAIVGANAFAHESGIHQDGMLK K TYEI+SPEDIGL RSN+SGIVLG
Sbjct: 365 VAEYSGLQVQPHKAIVGANAFAHESGIHQDGMLKHKETYEIISPEDIGLFRSNESGIVLG 424
Query: 426 KLSGRHALKSRLLELGYELDGKELDDVFWRFKAVA 322
KLSGRHALKSRLLELGYE++G ELDD+FWRFK VA
Sbjct: 425 KLSGRHALKSRLLELGYEIEGMELDDIFWRFKEVA 459
Score = 92.4 bits (228), Expect = 2e-16
Identities = 46/57 (80%), Positives = 50/57 (87%)
Frame = -2
Query: 173 STATVKLIGADGVEHVCCSVGTGPVDAAYKAIDLIVKVPVRLLEYSMNAVTEGIDAI 3
STATVKLI ADG +HV CSVGTGPVD+AYKAIDL+VK V LLEY +NAVTEGIDAI
Sbjct: 500 STATVKLIDADGQKHVACSVGTGPVDSAYKAIDLLVKEQVTLLEYGLNAVTEGIDAI 556
>sp|O04973.1|LEU1A_SOLPN RecName: Full=2-isopropylmalate synthase A; AltName: Full=Alpha-IPM
synthase A; AltName: Full=Alpha-isopropylmalate synthase
A gi|2213882|gb|AAB61598.1| 2-isopropylmalate synthase
[Solanum pennellii]
Length = 589
Score = 565 bits (1455), Expect = e-158
Identities = 269/335 (80%), Positives = 314/335 (93%)
Frame = -1
Query: 1326 EAGFPASSKDDLQAVKLIAQEVGNAVDGDGYVPVICGLARCNRSDIDAAWEAVKHAKRPR 1147
EAGFPASS+ D ++VKLIA+E+GN D +G+VPVICGL+RCN+SDID AWEAVK+AK+PR
Sbjct: 87 EAGFPASSEADFESVKLIAEEIGNNTDENGFVPVICGLSRCNKSDIDKAWEAVKYAKKPR 146
Query: 1146 IHTFIATSEIHLKYKLKKSRDEVVAIAREMVSYARSLGCNDIEFSPEDAGRSDREYLYEV 967
+HTFIATSEIH+KYKLK SR++VV AR MV+YARSLGC D+EFSPEDAGRSDRE+LY++
Sbjct: 147 VHTFIATSEIHMKYKLKMSREQVVEKARSMVAYARSLGCEDVEFSPEDAGRSDREFLYDI 206
Query: 966 LGEVIKAGATTLNIPDTVGYNLPIEFGKLIADIKSNTPGIENVIISTHCQNDLGLSTANT 787
LGEVIKAGATTLNIPDTVGY +P EFG+LI DIK+NTPGIENVIISTHCQNDLGLSTANT
Sbjct: 207 LGEVIKAGATTLNIPDTVGYTVPSEFGQLITDIKANTPGIENVIISTHCQNDLGLSTANT 266
Query: 786 IAGAHAGARQLEVTVNGIGERAGNASLEEVVMAIKCRGNELLSGLYTGVNTKHIVMASRM 607
+AGA AGARQLEVT+NGIGERAGNASLEEVVMA+KCRG ++L GLYTG+NT+HIV +S+M
Sbjct: 267 LAGACAGARQLEVTINGIGERAGNASLEEVVMALKCRGEQVLGGLYTGINTQHIVPSSKM 326
Query: 606 VAEYTGLQVQPHKAIVGANAFAHESGIHQDGMLKFKGTYEIMSPEDIGLSRSNDSGIVLG 427
V EY+GLQVQPHKAIVGANAFAHESGIHQDGMLK K TYEI+SP+D+GLSRSN++GIVLG
Sbjct: 327 VEEYSGLQVQPHKAIVGANAFAHESGIHQDGMLKHKDTYEIISPDDVGLSRSNEAGIVLG 386
Query: 426 KLSGRHALKSRLLELGYELDGKELDDVFWRFKAVA 322
KLSGRHALKS++LELGY++DGKEL+D+FWRFK+VA
Sbjct: 387 KLSGRHALKSKMLELGYDIDGKELEDLFWRFKSVA 421
Score = 101 bits (252), Expect = 4e-19
Identities = 49/57 (85%), Positives = 52/57 (91%)
Frame = -2
Query: 173 STATVKLIGADGVEHVCCSVGTGPVDAAYKAIDLIVKVPVRLLEYSMNAVTEGIDAI 3
STATVKLI DG EH+ CSVGTGPVDAAYKA+DLIVKVP+ LLEYSMNAVTEGIDAI
Sbjct: 462 STATVKLINTDGQEHIACSVGTGPVDAAYKAVDLIVKVPITLLEYSMNAVTEGIDAI 518
>gb|AAW81741.1| Putative 2-isopropylmalate synthase [Brassica oleracea]
Length = 623
Score = 564 bits (1453), Expect = e-158
Identities = 269/335 (80%), Positives = 308/335 (91%)
Frame = -1
Query: 1326 EAGFPASSKDDLQAVKLIAQEVGNAVDGDGYVPVICGLARCNRSDIDAAWEAVKHAKRPR 1147
EAGFPA+SKDD +AVK IA+ VGNAVDGDGYVPVICGL+RCN+ DI+ AWEAVK+AKRPR
Sbjct: 120 EAGFPAASKDDFEAVKTIAETVGNAVDGDGYVPVICGLSRCNKRDIETAWEAVKYAKRPR 179
Query: 1146 IHTFIATSEIHLKYKLKKSRDEVVAIAREMVSYARSLGCNDIEFSPEDAGRSDREYLYEV 967
IHTFIATS+IHL+YKLKKS+DEV+ AR MV +ARSLGC D+EFSPEDAGRS+RE+LY++
Sbjct: 180 IHTFIATSDIHLEYKLKKSKDEVIEFARNMVKFARSLGCEDVEFSPEDAGRSEREFLYQI 239
Query: 966 LGEVIKAGATTLNIPDTVGYNLPIEFGKLIADIKSNTPGIENVIISTHCQNDLGLSTANT 787
LGEVIKAGATTLNIPDTVG LP EFG+LIADIK+NTPGIENVIISTHCQNDLGLSTANT
Sbjct: 240 LGEVIKAGATTLNIPDTVGITLPSEFGQLIADIKANTPGIENVIISTHCQNDLGLSTANT 299
Query: 786 IAGAHAGARQLEVTVNGIGERAGNASLEEVVMAIKCRGNELLSGLYTGVNTKHIVMASRM 607
++GAH+GARQ+EVT+NGIGERAGNASLEEVVMAIKCRG+ +L GLYTG++T+HIVM S+M
Sbjct: 300 LSGAHSGARQVEVTINGIGERAGNASLEEVVMAIKCRGDHVLGGLYTGIDTRHIVMTSKM 359
Query: 606 VAEYTGLQVQPHKAIVGANAFAHESGIHQDGMLKFKGTYEIMSPEDIGLSRSNDSGIVLG 427
V +YTG+Q QPHKAIVGANAFAHESGIHQDGMLK KGTYEI+ PE+IGL RSND+GIVLG
Sbjct: 360 VEDYTGMQTQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIICPEEIGLERSNDAGIVLG 419
Query: 426 KLSGRHALKSRLLELGYELDGKELDDVFWRFKAVA 322
KLSGRHALK RL ELGY LD ++L +FWRFK+VA
Sbjct: 420 KLSGRHALKDRLTELGYVLDDEQLSSIFWRFKSVA 454
Score = 95.5 bits (236), Expect = 3e-17
Identities = 47/57 (82%), Positives = 50/57 (87%)
Frame = -2
Query: 173 STATVKLIGADGVEHVCCSVGTGPVDAAYKAIDLIVKVPVRLLEYSMNAVTEGIDAI 3
STATVKL ADG EHV CS+GTGPVD+AYKA+DLIVK P LLEYSMNAVTEGIDAI
Sbjct: 495 STATVKLADADGKEHVACSMGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAI 551
>gb|ABA26446.1| 2-isopropylmalate synthase 1 [Brassica insularis]
Length = 623
Score = 564 bits (1453), Expect = e-158
Identities = 270/335 (80%), Positives = 308/335 (91%)
Frame = -1
Query: 1326 EAGFPASSKDDLQAVKLIAQEVGNAVDGDGYVPVICGLARCNRSDIDAAWEAVKHAKRPR 1147
EAGFPA+SKDD +AVK IA VGNAVDGDGYVPVICGL+RCN+ DI+ AWEAVK+AKRPR
Sbjct: 120 EAGFPAASKDDFEAVKTIAGTVGNAVDGDGYVPVICGLSRCNKRDIETAWEAVKYAKRPR 179
Query: 1146 IHTFIATSEIHLKYKLKKSRDEVVAIAREMVSYARSLGCNDIEFSPEDAGRSDREYLYEV 967
IHTFIATS+IHL+YKLKKS+DEV+ IAR MV +ARSLGC D+EFSPEDAGRS+RE+LY++
Sbjct: 180 IHTFIATSDIHLEYKLKKSKDEVIEIARNMVKFARSLGCEDVEFSPEDAGRSEREFLYQI 239
Query: 966 LGEVIKAGATTLNIPDTVGYNLPIEFGKLIADIKSNTPGIENVIISTHCQNDLGLSTANT 787
LGEVIKAGATTLNIPDTVG LP EFG+LIADIK+NTPGIENVIISTHCQNDLGLSTANT
Sbjct: 240 LGEVIKAGATTLNIPDTVGITLPSEFGQLIADIKANTPGIENVIISTHCQNDLGLSTANT 299
Query: 786 IAGAHAGARQLEVTVNGIGERAGNASLEEVVMAIKCRGNELLSGLYTGVNTKHIVMASRM 607
++GAH+GARQ+EVT+NGIGERAGNASLEEVVMAIKCRG+ +L GLYTG++T+HIVM S+M
Sbjct: 300 LSGAHSGARQVEVTINGIGERAGNASLEEVVMAIKCRGDHVLGGLYTGIDTRHIVMTSKM 359
Query: 606 VAEYTGLQVQPHKAIVGANAFAHESGIHQDGMLKFKGTYEIMSPEDIGLSRSNDSGIVLG 427
V +YTG+Q QPHKAIVGANAFAHESGIHQDGMLK KGTYEI+ PE+IGL RSND+GIVLG
Sbjct: 360 VEDYTGMQTQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIICPEEIGLERSNDAGIVLG 419
Query: 426 KLSGRHALKSRLLELGYELDGKELDDVFWRFKAVA 322
KLSGRHALK RL ELGY LD ++L +FWRFK+VA
Sbjct: 420 KLSGRHALKDRLTELGYVLDDEQLSSIFWRFKSVA 454
Score = 95.5 bits (236), Expect = 3e-17
Identities = 47/57 (82%), Positives = 50/57 (87%)
Frame = -2
Query: 173 STATVKLIGADGVEHVCCSVGTGPVDAAYKAIDLIVKVPVRLLEYSMNAVTEGIDAI 3
STATVKL ADG EHV CS+GTGPVD+AYKA+DLIVK P LLEYSMNAVTEGIDAI
Sbjct: 495 STATVKLADADGKEHVACSMGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAI 551
>ref|NP_177544.1| 2-isopropylmalate synthase 2 [Arabidopsis thaliana]
gi|75168891|sp|Q9C550.1|LEU12_ARATH RecName:
Full=2-isopropylmalate synthase 2, chloroplastic;
AltName: Full=2-isopropylmalate synthase 1; AltName:
Full=Methylthioalkylmalate synthase-like 3; Flags:
Precursor gi|12325154|gb|AAG52530.1|AC016662_24 putative
2-isopropylmalate synthase; 30920-27612 [Arabidopsis
thaliana] gi|12330687|gb|AAG52882.1|AF327647_1
2-isopropylmalate synthase [Arabidopsis thaliana]
gi|16323111|gb|AAL15290.1| At1g74040/F2P9_9 [Arabidopsis
thaliana] gi|23463041|gb|AAN33190.1| At1g74040/F2P9_9
[Arabidopsis thaliana] gi|332197419|gb|AEE35540.1|
2-isopropylmalate synthase 2 [Arabidopsis thaliana]
Length = 631
Score = 562 bits (1449), Expect = e-158
Identities = 271/335 (80%), Positives = 308/335 (91%)
Frame = -1
Query: 1326 EAGFPASSKDDLQAVKLIAQEVGNAVDGDGYVPVICGLARCNRSDIDAAWEAVKHAKRPR 1147
EAGFPA+SKDD +AVK IA+ VGN VD +GYVPVICGL+RCN+ DI+ AWEAVK+AKRPR
Sbjct: 126 EAGFPAASKDDFEAVKTIAETVGNTVDENGYVPVICGLSRCNKKDIETAWEAVKYAKRPR 185
Query: 1146 IHTFIATSEIHLKYKLKKSRDEVVAIAREMVSYARSLGCNDIEFSPEDAGRSDREYLYEV 967
IHTFIATS+IHLKYKLKKS++EV+ IAR MV +ARSLGC D+EFSPEDAGRS+REYLYE+
Sbjct: 186 IHTFIATSDIHLKYKLKKSKEEVIEIARNMVRFARSLGCEDVEFSPEDAGRSEREYLYEI 245
Query: 966 LGEVIKAGATTLNIPDTVGYNLPIEFGKLIADIKSNTPGIENVIISTHCQNDLGLSTANT 787
LGEVIKAGATTLNIPDTVG LP EFG+LIADIK+NTPGI+NVIISTHCQNDLGLSTANT
Sbjct: 246 LGEVIKAGATTLNIPDTVGITLPSEFGQLIADIKANTPGIQNVIISTHCQNDLGLSTANT 305
Query: 786 IAGAHAGARQLEVTVNGIGERAGNASLEEVVMAIKCRGNELLSGLYTGVNTKHIVMASRM 607
++GAH+GARQ+EVT+NGIGERAGNASLEEVVMAIKCRG+ +L GL+TG++T+HIVM S+M
Sbjct: 306 LSGAHSGARQVEVTINGIGERAGNASLEEVVMAIKCRGDHVLGGLFTGIDTRHIVMTSKM 365
Query: 606 VAEYTGLQVQPHKAIVGANAFAHESGIHQDGMLKFKGTYEIMSPEDIGLSRSNDSGIVLG 427
V EYTG+Q QPHKAIVGANAFAHESGIHQDGMLK KGTYEIMSPE+IGL RSND+GIVLG
Sbjct: 366 VEEYTGMQTQPHKAIVGANAFAHESGIHQDGMLKHKGTYEIMSPEEIGLERSNDAGIVLG 425
Query: 426 KLSGRHALKSRLLELGYELDGKELDDVFWRFKAVA 322
KLSGRHALK RL ELGY LD +L ++FWRFKAVA
Sbjct: 426 KLSGRHALKDRLNELGYVLDDGQLSNLFWRFKAVA 460
Score = 95.5 bits (236), Expect = 3e-17
Identities = 47/57 (82%), Positives = 50/57 (87%)
Frame = -2
Query: 173 STATVKLIGADGVEHVCCSVGTGPVDAAYKAIDLIVKVPVRLLEYSMNAVTEGIDAI 3
ST+TVKL +DG EHV CSVGTGPVDAAYKA+DLIVK P LLEYSMNAVTEGIDAI
Sbjct: 501 STSTVKLADSDGKEHVACSVGTGPVDAAYKAVDLIVKEPATLLEYSMNAVTEGIDAI 557