BLASTX nr result
ID: Aconitum21_contig00003036
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Aconitum21_contig00003036
(2023 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002275565.1| PREDICTED: probable serine/threonine-protein... 469 e-175
ref|XP_002533407.1| kinase, putative [Ricinus communis] gi|22352... 452 e-152
ref|XP_002328479.1| predicted protein [Populus trichocarpa] gi|2... 446 e-150
ref|XP_003602560.1| Protein kinase [Medicago truncatula] gi|3554... 436 e-147
ref|NP_188505.1| putative serine/threonine-protein kinase WNK6 [... 456 e-146
>ref|XP_002275565.1| PREDICTED: probable serine/threonine-protein kinase WNK6-like [Vitis
vinifera]
Length = 677
Score = 469 bits (1207), Expect(2) = e-175
Identities = 232/341 (68%), Positives = 270/341 (79%), Gaps = 4/341 (1%)
Frame = +1
Query: 184 TGRYVRFEDVLGKGACKTVYKAFDEFDGIEVAWSQVPIDEFLRSPQDLERLHSEVHLLKV 363
T RY+RF+D+LGKGA KTVYKAFD+ DGIEVAW+QV IDE L+SP +LERL+SEVHLLK
Sbjct: 29 TSRYIRFKDILGKGAFKTVYKAFDQVDGIEVAWNQVRIDEVLQSPDELERLYSEVHLLKS 88
Query: 364 LKHKNIIKFYNFWVDDKNKTINIITELFTSGSLRQYRXXXXXXXXXXXXGWARQILTGLA 543
LKHKNIIKFYN W+DD NKT+NIITELFTSGSLRQYR GWARQIL GL
Sbjct: 89 LKHKNIIKFYNSWIDDGNKTVNIITELFTSGSLRQYRKKHKKVDMKAVKGWARQILMGLN 148
Query: 544 YLHSHNPPIIHRDLKCDNVFINGNHGEVKIGDLGFAAIMQQANAHSVIGTPQFMAPELYD 723
YLH+HNPPIIHRDLKCDN+FINGN GEVKIGDLG A +MQQANA +VIGTP+FMAPELYD
Sbjct: 149 YLHNHNPPIIHRDLKCDNIFINGNQGEVKIGDLGLATVMQQANARTVIGTPEFMAPELYD 208
Query: 724 EDYNELVDIYSFGMCMLEMVTFEYPYCECKNSAQIYKKVSSGIKPASLSKVNDPDVKLFI 903
E+YNEL DIYSFGMCMLEMVT EYPY EC+NSAQIYKKVS+GIKPA+LSK+ D +VK+FI
Sbjct: 209 ENYNELADIYSFGMCMLEMVTLEYPYSECRNSAQIYKKVSNGIKPAALSKIKDLEVKMFI 268
Query: 904 EKCLLPASQRASASELLKDPFLQVDVFSRNSSFNLPSIVSPKVGGSGNCSVLSEEPNNTR 1083
EKCL+PASQR SA +LL DPF QVD ++N LP IV PK G G+ +LSE P + +
Sbjct: 269 EKCLVPASQRLSAKKLLNDPFFQVDGLTKNHPLQLPDIVIPKTGAFGDRCLLSEGPTSLQ 328
Query: 1084 -RVSSMDVDTNGSDETPVITYVGETVIHRP---CLVIQVSK 1194
R +MD+D DE P+IT + +V P C+ +Q +K
Sbjct: 329 NRPLAMDLDAVDDDELPIITSMDNSVDGGPYSLCMEVQRAK 369
Score = 174 bits (440), Expect(2) = e-175
Identities = 117/276 (42%), Positives = 157/276 (56%), Gaps = 3/276 (1%)
Frame = +3
Query: 1203 FKLKGKMKGDGTISLKLRIAGLVDPAKVLHFRFYLCSDTSLSVAGEMVEQMEISDENVMC 1382
F LKG+ + +ISL LRIA + +HF FYL SDT+LSV+ EMVEQ+E++D+NV
Sbjct: 374 FLLKGEGNDENSISLILRIADQNGRLRNIHFMFYLDSDTALSVSSEMVEQLELADQNVTF 433
Query: 1383 IAELIDLSLINFIPTWKPSVPIHHLAAPNRNRISESHISDSQKPGERDFSSIVSFYNLFE 1562
IAELIDL LI IPTWKP VPI HL A NR + S H D Q P E + SF + E
Sbjct: 434 IAELIDLLLIMLIPTWKPCVPIDHLVALNRMQTSNGHHEDLQCP-EHGECLVGSFEGVCE 492
Query: 1563 AFNHSDVPPSTDSSIVEGSFQPTHEAPDYAKVDEIMCHVDFGSPNPVT-EDQGSNMSLVS 1739
N +S+ EG + E P + +DEI H D G P+ T ED GS+MS VS
Sbjct: 493 TDNLLSPHVYPNSTSFEGYIETMQENPKHLSLDEIKTHADLGLPSSATVEDHGSDMSYVS 552
Query: 1740 AHSAEGNHKMFSISTCMS-ELGYED-NGCEMKTKVGEVLSGSEIGACFTDKDMAINMGSS 1913
A S EG+ K +S + +S E G D N K V + LS + +C DK A ++GS+
Sbjct: 553 ATSNEGSDKKYSHNAYLSAESGCMDYNEYGSKRGVRQSLSAVQTSSCNLDKGKATDIGSN 612
Query: 1914 DMPIHSNPPCNTSSIFVIDNDDEELRMELKQIELQY 2021
S+ P ++S +++ E + +EL+ IELQY
Sbjct: 613 GAVTSSDYPIDSSLSDQVES--ENMILELEMIELQY 646
>ref|XP_002533407.1| kinase, putative [Ricinus communis] gi|223526752|gb|EEF28980.1|
kinase, putative [Ricinus communis]
Length = 662
Score = 452 bits (1162), Expect(2) = e-152
Identities = 219/319 (68%), Positives = 261/319 (81%), Gaps = 1/319 (0%)
Frame = +1
Query: 184 TGRYVRFEDVLGKGACKTVYKAFDEFDGIEVAWSQVPIDEFLRSPQDLERLHSEVHLLKV 363
TGRY+R++++LGKGA K VYKAFDE +G+EVAW+QV IDE L+SP+DLERL+SEVHLLK
Sbjct: 24 TGRYIRYKEMLGKGAFKRVYKAFDEVNGLEVAWNQVRIDEVLQSPEDLERLYSEVHLLKS 83
Query: 364 LKHKNIIKFYNFWVDDKNKTINIITELFTSGSLRQYRXXXXXXXXXXXXGWARQILTGLA 543
LKH NI++FYN W+DDKNKT+NIITELFTSG+LR+Y GWARQIL GL+
Sbjct: 84 LKHGNIVRFYNSWIDDKNKTVNIITELFTSGNLREYCKKYRNVEMKAVKGWARQILMGLS 143
Query: 544 YLHSHNPPIIHRDLKCDNVFINGNHGEVKIGDLGFAAIMQQANAHSVIGTPQFMAPELYD 723
YLH H PP+IHRDLKCDN+FINGN GEVKIGDLG A IM+Q+NA SVIGTP+FMAPELYD
Sbjct: 144 YLHGHKPPVIHRDLKCDNIFINGNQGEVKIGDLGLATIMEQSNAKSVIGTPEFMAPELYD 203
Query: 724 EDYNELVDIYSFGMCMLEMVTFEYPYCECKNSAQIYKKVSSGIKPASLSKVNDPDVKLFI 903
EDYNEL DIYSFGMCMLEMVTFEYPY EC+NSAQIYKKVSSGIKPA+LSKV DP+VKLFI
Sbjct: 204 EDYNELADIYSFGMCMLEMVTFEYPYSECRNSAQIYKKVSSGIKPAALSKVKDPEVKLFI 263
Query: 904 EKCLLPASQRASASELLKDPFLQVDVFSRNSSFNLPSIVSPKVGGSGNCSVLSEEPNNTR 1083
+KC++ ASQR SA ELL DPFL+V+ ++N +LP IV PK+ G+ ++SE P N R
Sbjct: 264 QKCIVSASQRLSAKELLMDPFLEVNGLAKNRPLSLPDIVMPKLSAFGDRCLMSEGPANAR 323
Query: 1084 -RVSSMDVDTNGSDETPVI 1137
+ SMD+D + E P+I
Sbjct: 324 NKPPSMDLDND--PEMPII 340
Score = 114 bits (284), Expect(2) = e-152
Identities = 103/287 (35%), Positives = 139/287 (48%), Gaps = 14/287 (4%)
Frame = +3
Query: 1203 FKLKGKMKGDGTISLKLRIAGLVDP-AKVLHFRFYLCSDTSLSVAGEMVEQMEISDENVM 1379
F LKG+ + ++SL LRIA + +HF FYL DT+L V+ EMVEQ+E+ D+N+
Sbjct: 367 FFLKGEKHDENSVSLILRIADQNGSRVRNIHFLFYLDGDTALLVSSEMVEQLELEDQNIT 426
Query: 1380 CIAELIDLSLINFIPTWKPSVPIHHLAAPNRNRISESHISDSQKPGERDFSSIV---SFY 1550
IAELIDL L+ IP WKP V I HL N S+ ++ S K E IV S
Sbjct: 427 FIAELIDLLLLKLIPNWKPCVRIEHLVPRNATNSSKGNLK-SHKSSENYKDQIVVAGSAQ 485
Query: 1551 NLFEAFNHSDVPPSTDSSIVEGSFQPTHEAPDYAKVDEIMCHVDFGSPN-------PVTE 1709
++ + +S DS GS P EA I N + E
Sbjct: 486 DIRQIATYSRTSSCLDSCSQGGSI-PRSEADQDGSAGIIKLSDMPSQTNESGNYRMAMVE 544
Query: 1710 DQGSNMSLVSAHSAEGNHKMFSISTCM-SELGYED-NGCEMKTKVGEVLSGSEIGACFTD 1883
D S S SA S+E N K S ++C+ +E G + +G K G++L+ E G D
Sbjct: 545 DLVSEKSYTSAASSEWNDKRLSFNSCVTAESGLVNFDGHGFKGSQGDLLASMEAGQP-PD 603
Query: 1884 KDMAINMGSSDMPIHSNPPCNTSSIFVID-NDDEELRMELKQIELQY 2021
M I SSD P TS F+ + N+ ELR EL++IELQY
Sbjct: 604 SHMEI---SSDYP--------TSISFLSNKNESNELREELEKIELQY 639
>ref|XP_002328479.1| predicted protein [Populus trichocarpa] gi|222838194|gb|EEE76559.1|
predicted protein [Populus trichocarpa]
Length = 583
Score = 446 bits (1146), Expect(2) = e-150
Identities = 225/340 (66%), Positives = 262/340 (77%), Gaps = 3/340 (0%)
Frame = +1
Query: 184 TGRYVRFEDVLGKGACKTVYKAFDEFDGIEVAWSQVPIDEFLRSPQDLERLHSEVHLLKV 363
T RY+R+++VLGKGA KTVYKAFDE +GIEVAW+QV IDE L+SP DLERL+SE+HLLK
Sbjct: 24 TCRYIRYKEVLGKGAFKTVYKAFDEVNGIEVAWNQVRIDEVLQSPDDLERLYSEMHLLKT 83
Query: 364 LKHKNIIKFYNFWVDDKNKTINIITELFTSGSLRQYRXXXXXXXXXXXXGWARQILTGLA 543
LK+ NI++FYN W+DDK KT+NIITELFTSGSLRQY GWARQIL GL
Sbjct: 84 LKNSNIVRFYNSWIDDKKKTVNIITELFTSGSLRQYCKKHRKIEMKAVKGWARQILNGLN 143
Query: 544 YLHSHNPPIIHRDLKCDNVFINGNHGEVKIGDLGFAAIMQQANAHSVIGTPQFMAPELYD 723
YLH+H+PPIIHRDLKCDN+FINGN GEVKIGDLG A +M+QANA SVIGTP+FMAPELYD
Sbjct: 144 YLHNHDPPIIHRDLKCDNIFINGNQGEVKIGDLGLATVMEQANAKSVIGTPEFMAPELYD 203
Query: 724 EDYNELVDIYSFGMCMLEMVTFEYPYCECKNSAQIYKKVSSGIKPASLSKVNDPDVKLFI 903
E+YNEL DIYSFGMCMLEMVTFEYPY EC+NSAQIYKKVSSGIKPASLSKV DPD+K FI
Sbjct: 204 ENYNELADIYSFGMCMLEMVTFEYPYSECRNSAQIYKKVSSGIKPASLSKVKDPDLKQFI 263
Query: 904 EKCLLPASQRASASELLKDPFLQVDVFSRNSSFNLPSIVSPKVGGSGNCSVLSEEPNNTR 1083
EKCLLPAS+R A ELL DPFL V+ F+RNSS I+ K+ G+ ++SE P T
Sbjct: 264 EKCLLPASERLPAKELLMDPFLDVNGFARNSSIPRSDILMSKMTAIGDRCLMSEGP-ATA 322
Query: 1084 RVSSMDVDTNGSDETPVITYVGETV---IHRPCLVIQVSK 1194
R +D + E P IT +V H CL ++ +K
Sbjct: 323 RNKPPSMDLSHDPEMPTITSFNNSVDEDTHSLCLEVRRAK 362
Score = 113 bits (283), Expect(2) = e-150
Identities = 74/180 (41%), Positives = 103/180 (57%), Gaps = 4/180 (2%)
Frame = +3
Query: 1203 FKLKGKMKGDGTISLKLRIAGLVDPAKV--LHFRFYLCSDTSLSVAGEMVEQMEISDENV 1376
F L+G+ + ++SL LR+A + A+V +HF FYL DT+LSV+ EMVEQ+E+ ENV
Sbjct: 367 FLLRGEGNDENSVSLILRMADQ-NGARVRNIHFLFYLDGDTALSVSSEMVEQLELEVENV 425
Query: 1377 MCIAELIDLSLINFIPTWKPSVPIHHLAAPNRNRISESHISDSQKPGERDFSSIVSFYNL 1556
IAELIDL L+ IP WKP V I HL P+R + D G ++ F N
Sbjct: 426 KFIAELIDLLLLKLIPNWKPGVHIEHLVPPSREQTPRVQSKDFHSLGNGKM-AVAPFQNA 484
Query: 1557 FEAFNHSDVPPSTDSSIVEGSFQPTHEAPDYAKVDEIMCHV-DFGSPN-PVTEDQGSNMS 1730
+A NHS S+ + + GS E+P K+D++M ++ DF S N P ED+ S MS
Sbjct: 485 HDAANHSRC--SSRPNSLGGSIPTVGESPSTVKLDDLMSNLDDFDSQNPPAAEDRHSEMS 542
>ref|XP_003602560.1| Protein kinase [Medicago truncatula] gi|355491608|gb|AES72811.1|
Protein kinase [Medicago truncatula]
Length = 675
Score = 436 bits (1120), Expect(2) = e-147
Identities = 214/329 (65%), Positives = 255/329 (77%)
Frame = +1
Query: 184 TGRYVRFEDVLGKGACKTVYKAFDEFDGIEVAWSQVPIDEFLRSPQDLERLHSEVHLLKV 363
T RY+R+++V+GKGA KTVYKAFDE G+EVAWSQV IDE L+SP DLERL+SEVHLL+
Sbjct: 47 TCRYLRYKEVIGKGAFKTVYKAFDEVTGLEVAWSQVRIDEVLQSPGDLERLYSEVHLLRS 106
Query: 364 LKHKNIIKFYNFWVDDKNKTINIITELFTSGSLRQYRXXXXXXXXXXXXGWARQILTGLA 543
LKH NII+FYN W+DDK+ +N+ITELFTSGSLRQYR GWARQIL GL
Sbjct: 107 LKHSNIIRFYNSWIDDKHGNVNMITELFTSGSLRQYRKKHKKVDVKAVKGWARQILMGLV 166
Query: 544 YLHSHNPPIIHRDLKCDNVFINGNHGEVKIGDLGFAAIMQQANAHSVIGTPQFMAPELYD 723
YLH+HNPPIIHRD+KCDN+FING+ GEVKIGDLG A +++Q NA SVIGTP+FMAPELYD
Sbjct: 167 YLHNHNPPIIHRDVKCDNIFINGHQGEVKIGDLGLATLLKQNNAKSVIGTPEFMAPELYD 226
Query: 724 EDYNELVDIYSFGMCMLEMVTFEYPYCECKNSAQIYKKVSSGIKPASLSKVNDPDVKLFI 903
EDYNEL DIYSFGMCMLE+ T +YPY EC+NSAQIYKKVSSGIKP SLS + DP++K FI
Sbjct: 227 EDYNELADIYSFGMCMLELATSQYPYRECRNSAQIYKKVSSGIKPVSLSTIKDPEIKSFI 286
Query: 904 EKCLLPASQRASASELLKDPFLQVDVFSRNSSFNLPSIVSPKVGGSGNCSVLSEEPNNTR 1083
EKCL+PASQR SA ELL DPFL+V+ +N LP IV PK GG N ++SE P +
Sbjct: 287 EKCLVPASQRLSAKELLMDPFLEVNFSVKNRPLPLPDIVLPKFGGFENRCLMSEGPASA- 345
Query: 1084 RVSSMDVDTNGSDETPVITYVGETVIHRP 1170
R+ S ++ ++E PVIT +V P
Sbjct: 346 RIGSSSMELGDTNEQPVITVSYNSVDDAP 374
Score = 115 bits (288), Expect(2) = e-147
Identities = 97/283 (34%), Positives = 135/283 (47%), Gaps = 10/283 (3%)
Frame = +3
Query: 1203 FKLKGKMKGDGTISLKLRIAGLVDPAKVLHFRFYLCSDTSLSVAGEMVEQMEISDENVMC 1382
F LKG+ +ISL LRI A+ +HF FYL SDT++SV+ EMVEQ+E+++ NV
Sbjct: 390 FFLKGEANDTNSISLVLRIIDQGGRARNIHFIFYLDSDTAMSVSSEMVEQLELAEHNVKF 449
Query: 1383 IAELIDLSLINFIPTWKPSVPIHHLAAPNRNRISESHISDSQ--------KPGERDFSSI 1538
IAELIDL L IP WKP V I HL +PN R +H+S + K D S I
Sbjct: 450 IAELIDLLLTTLIPDWKPCVAIDHLISPNGRR---THMSQQKQLSQLAKYKQNSIDSSQI 506
Query: 1539 VSFYNLFEAFNHSDVPPSTDSSIVEGSFQPTHEAPDYAKVDEIMCHVDFGSPNPVTEDQG 1718
+ Y V PST + E D +++ H G D
Sbjct: 507 MREY----------VGPSTSHERL-----AEKENIDNMNFEDVFSHASIGLQRTTKTDDL 551
Query: 1719 SNMSLVSAHSAEGNHKMFSISTCMS-ELGYEDNGCEMKTKVGEVLS-GSEIGACFTDKDM 1892
+M+ ++ +++ N K FS + MS G+ D G S SEIGA + K
Sbjct: 552 YSMTSYTSATSDYNDKNFSTVSFMSARSGFTDFNFPTVNGWGSQSSLASEIGASYDKKSK 611
Query: 1893 AINMGSSDMPIHSNPPCNTSSIFVIDNDDEELRMELKQIELQY 2021
M ++N P +TSS ++ELR+EL++IE QY
Sbjct: 612 FPCME------NNNYPLSTSS---FHEAEDELRIELEKIERQY 645
>ref|NP_188505.1| putative serine/threonine-protein kinase WNK6 [Arabidopsis thaliana]
gi|334185446|ref|NP_001189928.1| putative
serine/threonine-protein kinase WNK6 [Arabidopsis
thaliana] gi|75248034|sp|Q8S8Y8.1|WNK6_ARATH RecName:
Full=Probable serine/threonine-protein kinase WNK6;
Short=AtWNK6; AltName: Full=Protein kinase with no lysine
6 gi|20302604|dbj|BAB91129.1| Ser/Thr kinase [Arabidopsis
thaliana] gi|51970136|dbj|BAD43760.1| putative mitogen
activated protein kinase kinase [Arabidopsis thaliana]
gi|133778828|gb|ABO38754.1| At3g18750 [Arabidopsis
thaliana] gi|332642618|gb|AEE76139.1| putative
serine/threonine-protein kinase WNK6 [Arabidopsis
thaliana] gi|332642620|gb|AEE76141.1| putative
serine/threonine-protein kinase WNK6 [Arabidopsis
thaliana]
Length = 567
Score = 456 bits (1173), Expect(2) = e-146
Identities = 220/320 (68%), Positives = 255/320 (79%)
Frame = +1
Query: 184 TGRYVRFEDVLGKGACKTVYKAFDEFDGIEVAWSQVPIDEFLRSPQDLERLHSEVHLLKV 363
T RY+R+++V+GKGA KTVYKAFDE DGIEVAW+QV ID+ L+SP LERL+SEV LLK
Sbjct: 24 TFRYIRYKEVIGKGAFKTVYKAFDEVDGIEVAWNQVRIDDVLQSPNCLERLYSEVRLLKS 83
Query: 364 LKHKNIIKFYNFWVDDKNKTINIITELFTSGSLRQYRXXXXXXXXXXXXGWARQILTGLA 543
LKH NII+FYN W+DDKNKT+NIITELFTSGSLR YR WARQIL GL
Sbjct: 84 LKHNNIIRFYNSWIDDKNKTVNIITELFTSGSLRHYRKKHRKVNMKAVKNWARQILMGLR 143
Query: 544 YLHSHNPPIIHRDLKCDNVFINGNHGEVKIGDLGFAAIMQQANAHSVIGTPQFMAPELYD 723
YLH PPIIHRDLKCDN+FINGNHGEVKIGDLG A +M+QANA SVIGTP+FMAPELYD
Sbjct: 144 YLHGQEPPIIHRDLKCDNIFINGNHGEVKIGDLGLATVMEQANAKSVIGTPEFMAPELYD 203
Query: 724 EDYNELVDIYSFGMCMLEMVTFEYPYCECKNSAQIYKKVSSGIKPASLSKVNDPDVKLFI 903
E+YNEL DIYSFGMCMLEMVTF+YPYCECKNSAQIYKKVSSGIKPASLS+V DP+VK FI
Sbjct: 204 ENYNELADIYSFGMCMLEMVTFDYPYCECKNSAQIYKKVSSGIKPASLSRVKDPEVKQFI 263
Query: 904 EKCLLPASQRASASELLKDPFLQVDVFSRNSSFNLPSIVSPKVGGSGNCSVLSEEPNNTR 1083
EKCLLPAS+R SA ELL DPFLQ++ + N+ LP IV PK G G+ ++SE P TR
Sbjct: 264 EKCLLPASERLSAKELLLDPFLQLNGLTMNNPLPLPDIVMPKEGAFGDRCLMSEGPPTTR 323
Query: 1084 RVSSMDVDTNGSDETPVITY 1143
++ +D + P++T+
Sbjct: 324 PSKTLSIDLDEDSNLPIVTF 343
Score = 90.9 bits (224), Expect(2) = e-146
Identities = 59/163 (36%), Positives = 87/163 (53%), Gaps = 9/163 (5%)
Frame = +3
Query: 1203 FKLKGKMKGDGTISLKLRIAGLVDPAKVLHFRFYLCSDTSLSVAGEMVEQMEISDENVMC 1382
F LKG+ + ++SL LRI + +HF FY DT+ V+ EMVEQ+E++D+NV
Sbjct: 363 FVLKGEENDEQSVSLILRIVDENGRVRNIHFLFYQEGDTASKVSSEMVEQLELTDQNVTF 422
Query: 1383 IAELIDLSLINFIPTWKPSVPIHHLAAPNRNRISESHISDSQKPGERDFSSIVSFYNLFE 1562
IAELID+ L+N IPTWK V + HL N+ S SH +++ KP +++ + L
Sbjct: 423 IAELIDILLVNMIPTWKTDVTVDHLIHSQLNQNSRSHHNEA-KPQKQEETVFHDTCELVS 481
Query: 1563 AFNHSDVPPS--TDSSIVE-------GSFQPTHEAPDYAKVDE 1664
+SD P S D V+ S Q EA + ++E
Sbjct: 482 HSCNSDCPRSDEEDKQCVDATKGEDKSSIQEVEEATEPVSLEE 524